2014 Abstracts

Mark Soffe, Utah State University Life Sciences SSBs are DNA binding proteins that are essential components of cells and play key roles in DNA replication, repair, and recombination. Here we utilize two biochemical properties associated with the E. coli SSB protein to develop a novel procedure to purify proteins using a resin-free strategy. 1. E. coli SSB binds to single stranded DNA (ssDNA) with extremely high affinity (K = 1013 M-1), indicating very tight binding. 2. It is also a unique protein with respect to its purification – it is possible to obtain greater than 95% pure SSB from the total cell lysate without using any sort of column or resin, utilizing polyethyleneimine (PEI) and ammonium sulfate precipitation. Our design uses SSB as an affinity/solubility tag to enhance the solubility and expression of difficult-to-purify proteins, and allows for the simple, resin-free purification using PEI and ammonium sulfate precipitation. There also may be a possibility to co-express protein dimers and possibly tetramers using this method. Constructs have been made that include the SSB gene, along with the ability to fuse any gene of interest, as well as a TEV Protease cleavage sequence allowing for proteolytic cleavage after gene expression. Two genes of interest have been cloned in thus far—TEV protease and Rad51. In this proposal I outline experiments to develop this strategy further and test our proof of principle concept and its application to a broader set of target proteins.

Marilize Van der Walt, Utah State University Life Sciences Urbanization is accompanied by a lot of changes to the landscape that have the potential to affect the native species inhabiting the area. If animals are chronically exposed to these anthropogenic disturbances and are unable to acclimatize, changes in circulating glucocorticoid hormones may cause adverse effects to the animal’s health, such as an impairment in innate immune activity. One such disturbance is human-induced noise. Using the side-blotched lizard, Uta stansburiana, because of their localized habitat and inability to escape such disturbances within the urban environment, we looked at the field and lab components of noise as a stressor. We first measured decibel levels in urban and rural field sites in their natural habitats in St. George, Utah, and conducted a lab study exposing U. stansburiana to either a synthesized urban sound recording or no sound recording (control) for nine days. We collected blood samples and measured circulating corticosterone and testosterone concentrations and bactericidal ability to determine if there are endocrine and immune alterations in response to increased noise decibels. Our results show that lizards exposed to urban noise experienced increased corticosterone levels indicative of stress. Furthermore, bactericidal ability was indirectly affected by noise through significant correlations between body condition and corticosterone and testosterone concentrations. These results indicate that an increase in ambient decibel levels acts as a stressor to animals in urban areas. By dissecting out an individual component of a complex stressor we can better understand the effects of urbanization as a whole.

Molly Van Engelenhoven, Utah State University Life Sciences For thousands of years farmers have known that soil organisms can destroy crops but only in the past ten years have researchers begun to understand that complex interactions among soil organisms and plants can determine plant diversity and productivity. Much of the research in this young field of study has come from qualitative greenhouse experiments and separately from simple mathematical models. Here, we report findings from a six-year plant-soil feedback (PSF) field experiment that assessed the effects of soil organisms on the growth of twelve plant species common to the intermountain west. Initially, twelve native and exotic species were randomly assigned to 1550 experimental plots in a field. After four years of growth, the plants were killed. Next, the same plant species were randomly assigned to the 1550 plots again to test how each plant species responded to the soil microbial communities ‘cultivated’ by each other plant species. Two years later plant growth responses to different soil types were measured. Most plants showed large growth differences on different soil types. For example, Bromus tectorum grew twice as much on Pseudoroegneria spicata-cultivated soils than on self-cultivated soils. When the plant growth responses observed in this experiment were used in a mathematical model, PSFs were found to play a critical role in determining which plants were likely to be dominant in a community and how productive these different plant communities would be. In summary, this large-scale experiment provides strong evidence for the role of PSFs in plant productivity and diversity. Results have implications for natural methods of effective native plant restoration, biofuel production and weed control.

Michael Morgan, Utah State University Life Sciences The advancement of biologically derived alternatives to petroleum diesel fuel requires a multifaceted approach. At Utah State University we use an interdisciplinary team including the Colleges of Engineering, Agriculture & Applied Sciences, and Science in conjunction with industry partners to drive innovation in improving the science behind petroleum diesel alternatives. With increasing petroleum use, depleting reserves, increasing emissions standards, and other factors, there is need for petroleum diesel alternatives that are cost effective, offer improvement, and perform similarly to petroleum diesel. Our team has focused on the use of oleaginous microbes utilizing low value effluent and waste sources including sugars and CO2 to create biofuels. We have focused on a yeast, Cryptococcus curvatus, and a microalgae, Nannochloropsis salina which have shown high yields of fuel per cell mass. Using these microbes we have utilized USU’s own direct trans-esterification reaction to create sufficient quantities of biodiesel for engine performance and emissions testing, including a subset of ASTM tests characterizing the fuels from each organism. Our initial engine testing used petroleum diesel as a baseline in conjunction with commercial soybean biodiesel to establish the quality of our microbially derived biodiesel. Testing in stationary diesel engines and on the Bonneville Salt Flats has proven our microbial fuels perform similarly to soybean biodiesel and comparably to petroleum diesel. To further improve biological diesel replacements we have begun working to create green diesel, hydrocarbons from a biological source, using a novel method of hydrothermal liquefaction. Preliminary results of those tests are presented here. Through a multifaceted and interdisciplinary approach USU is successfully improving petroleum diesel alternatives from microbial sources including characterization of the properties of these fuels and is working to create the fuels at the scale necessary for exhaustive engine performance and emissions testing including ASTM testing of all important fuel properties.

Michaelle Cadet, Utah Valley University Life Sciences Utah County, Utah has an estimated population of 540,000 residents and is considered to be a non-attainment area for criteria pollutants such as PM-10 and CO. High levels of these contaminants may increase the risk of respiratory diseases. Additional environmental issues exist including water contamination and eutrophication of Utah Lake. These environmental issues are frequently on the news and warnings are issued by the Department of Environmental Quality notifying citizens of the potential health concerns associated with environmental pollution. With these announcements, it is expected that Utahans are acutely aware of environmental issues, particularly, in the academic settings. The purpose of this study was to investigate the status of environmental awareness among students at Utah Valley University and to correlate the results to socio-demographic categories. Utah Valley University, located in Utah County, is a public institution of higher education with approximately 30,000 registered students. Data for this study was collected through a survey using a multistage sampling technique with population stratified based on colleges and schools within the university. Sample size included approximately 1,000 students. The survey contained 12 environmental questions relating to recent local, national and international media exposure. Standards and protocol of the International Research Board were employed. We hypothesize that married, educated, religious males will positively correlate with higher levels of environmental awareness, but will be less than atheists. Additionally, those identifying with the Republican Party will have lower levels of awareness. No significant difference will be found between majors. Furthermore the young, poor and ethnic populations will be less aware than their more affluent counterparts. Results of this study will be used to inform residents of environmental issues and the associated health concerns. Additionally, this study will be used to inform legislators about the importance of environmental education in the community.

Jeremy Rehm, Brigham Young University Life Science The recent surge of interest in personality differences between individuals of a single population or members of differing populations has generated numerous new hypotheses that may aid in elucidating patterns of ecology and evolution that were previously considered improbable. Two hypotheses relevant to fish biology relate the size of an organism from a certain predation environment to the level of boldness it exhibits. The first of these (predation hypothesis) predicts small individuals living with predators should not express boldness comparable to their larger counterparts, whereas the other (metabolic hypothesis) predicts the exact opposite. Our study investigated these hypotheses using two sister-taxa fish species in Panama (Brachyrhaphis roseni and B. terrabensis) that exhibit two size classes (large and small) and live in differing predation environments. Additionally, because males are smaller than females in both species, we could look at size-boldness relations within each species. The study, as in others, defined boldness as the amount of time for an individual to emerge from a shelter and into an unfamiliar territory. When the species are analyzed collectively, our results support previous findings that fish from high-predation environments tend to be bolder than those without predators; males tend to be bolder than females; and both mass and standard length positively correlate with boldness. However, within species analyses find that mass and standard length have no significant relation to boldness, and gender was only significant in the predation-exposed B.roseni, where males were bolder. These interesting findings contrast with previous studies, and lead us to question the value of these size-related hypotheses in the process of speciation and, ultimately, evolution.

Ethel Tackie-Yarboi, Westminster College Life Sciences The brine shrimp, Artemia franciscana are an important source of nutrients for many of the over 15 million migratory birds that visit Great Salt Lake (GSL) in Utah each year. As well as being a source of food, the brine shrimp are an intermediate host to cestode parasites that infect many of the bird species. Although the effects of cestode infection in brine shrimp and the contribution of brine shrimp to cestode circulation in birds have been studied, little research has yet addressed the rate of cestode infection or molecular phylogeny of these cestodes. We collected brine shrimp from three sites in GSL and tested individuals for cestode infection using previously identified and newly designed cestode specific PCR primers that amplify the 18S ribosomal RNA gene. Our preliminary results showed that the Spiral Jetty site from the North arm of GSL had a 2% infection rate, the Antelope Island site from the South arm had a 45% infection rate, and the shrimp collected at the Black Rock site, also in the South arm, had a 32% infection rate using our cestode specific PCR assays. We also have preliminary DNA sequencing results that demonstrate that we are amplifying cestode DNA, which is most closely related to members of the genus Hymenolepis. Our data suggest that there may be a higher prevalence of cestode infection in the shrimp in the South arm than those from the North arm of the Great Salt Lake. Our goal is to extend our study in order to better identify the species of cestodes that infect brine shrimp using further DNA sequencing and to expand our infection rate samples to better estimate the percentage of the brine shrimp population that is infected by the cestode parasites, as well as test samples from several years.

J David Symons, University of Utah Life Sciences Cardiovascular complications (e.g., arterial dysfunction) are more prevalent in patients with type 2 diabetes (T2DM). Patients with T2DM have elevated levels of circulating free fatty acids (FFAs). We have shown that when bovine aortic endothelial cells (BAECs) are treated with the physiologically relevant FFA palmitate, protein phosphatase 2A (PP2A) activity increases, phosphorylated endothelial nitric oxide (NO) synthase (eNOS) to total eNOS (p-eNOS:eNOS) decreases, and metabolites of NO production decrease. NO is an important endothelial-derived relaxing factor that is vasculoprotective. As such, FFA-induced, PP2A-mediated reductions in p-eNOS:eNOS and NO production might explain why vascular complications are more common in pathologies associated with lipotoxicity e.g., T2DM and diet-induced obesity. Recently we showed in BAECs that if PP2A is inhibited using okadaic acid (OA), palmitate-induced increases in PP2A activity, and reductions in p-eNOS:eNOS and indices of NO production are negated. We sought to translate these finding from BAECs to the intact organism. However, OA cannot be used in vivo. Lixte Biotechnology 1 (LB1) is a PP2A inhibitor that has been used in vivo in the context of cancer research. The purpose of this study was to determine the efficacy of LB1 under our experimental conditions, with the long-range goal of using LB1 in mice. Further, we used this opportunity to optimize the measurement of NO directly using electron paramagnetic resonance spectroscopy (EPR). BAECs were treated for 3 h with vehicle (V), 500 µM palmitate (P), 4 µM LB1, or P + LB1 (n=10 per treatment). P increased (p<0.05) PP2A activity (50±12%), and decreased (p<0.05) p-tyr307:PP2A (29±9%; redundant indicator of increased PP2A activity), p-eNOS:eNOS (30±3%), and NO production (27±9%). All P-induced effects were prevented by concurrent treatment with LB1. Future experiments will determine whether chronic treatment of mice with LB1 is capable of suppressing PP2A activity in intact arteries.

Cynthiann Heckelsmiller, Weber State University Life Sciences Heavy foot traffic compacts soil, leading to decreased gas exchange, water permeability, and reduced nutrient cycling. Plant communities are defined by the availability of nutrients, water, and other resources.

Jordan Fenlon, University of Utah Life Sciences Tsr, the serine chemoreceptor for E. coli, is a transmembrane protein with a periplasmic sensing domain and cytoplasmic adaptation and kinase control domains. The focus of my research project is Tsr residue A413, located in the cytoplasmic tip of the receptor’s kinase control domain. The project involves characterization of mutant Tsr proteins containing amino acid replacements at residue 413. Based on previous work in the Parkinson lab, this residue is thought to play a key role in Tsr signaling by regulating the dynamic motion of the tip.

Charlee Byers, Brigham Young University Life Sciences Agave utahensis (Utah agave) plays a critical role as a keystone species in its native habitat. A rise in frequent, intense fires across the range of these habitats threatens to eliminate Utah agave populations, and consequently limit its genetic diversity. Characterizing the genetic diversity of Utah agave and its subspecies will help in restoration efforts to protect the species. We constructed primers to amplify microsatellite markers of two subspecies of Utah agave, ssp. kaibabensis and ssp. utahensis. Using these markers, we determined the level of polymorphism within four populations of each of the two subspecies.

Sean Studstill, Weber State University Life Sciences Imidacloprid is a popular systemic insecticide that has been applied to our staple crops for two decades. According to the EPA, it is persistent in the environment and at risk of effecting non-targeted organisms. Imidacloprid is an insect neurotoxin; however it is also known to be toxic to various aquatic species in concentrations as low as 37 ppb. Ingestion of imidacloprid causes paralysis in organisms through the blockage of postsynaptic nicotinic cholinergic receptors. We sought to find out how toxic imidacloprid is to brine shrimp and what kinds of physiological reactions occur upon exposure.

Evan Campbell, Brigham Young University Life Sciences Asthma is a chronic allergic disorder manifest by airway restriction due to inflammation, bronchoconstriction, and increased respiratory mucous secretion. As many as 300 million people worldwide are affected by asthma and its prevalence is increasing primarily in countries experiencing urbanization and Westernization. Asthma is currently the most common chronic illness among children in the U.S., and the third leading cause of hospitalization for children aged 0 – 15 yrs. Reservoir dust collection and area air sampling are the two primary methods of measuring allergen levels in house dust. Allergen sensitization leading to asthma is thought to occur prior to age six while the immune system is still naïve. In the case of the dust mite allergen Der p1, the exposure window may be as early as age two. However, little evidence is available to establish a dose–response relationship between inhalation exposure and early immunological sensitization to allergens. Temperature and relative humidity play a major role in dust mite survival and proliferation and indoor humidity above 50-60% in arid environments has been shown to support dust mite populations. Evaporative “swamp” coolers cool air by adding humidity to it and can create favorable environments for dust mite survival. We are working to determine how much swamp coolers contribute to dust mite levels in Utah by quantitating dust mite allergen levels in homes with and without swamp coolers. In order to do this we are developing sensitive ELISA and quantitative PCR methods to allow us to determine levels of exposure even when low levels of dust are collected.

Madison Peterson, Utah Valley University Life Sciences High-frequency (HF) ultrasound in the 20-80 MHz range has recently been found to be sensitive to pathology in tissue margins from breast cancer surgery. In order to improve the resolution and sensitivity of this method, however, transducers need to be employed that have piezoelectric elements that are smaller than those currently in use. The purpose of this study was to determine if small element transducers (Blatek pachyometer, 50 MHz, element diameter < 2 mm) produce similar results as those obtained from large element immersion transducers (Olympus NDT, V358-SU, 50 MHz, 6.35-mm diameter active element). Ultrasonic tests were performed on 10 phantom breast samples made of Knox gelatin base and soluble fiber (Metamucil), five of which contained chopped nylon fibers and five which contained polyethylene microspheres. Pulse-echo and through transmission measurements using a HF square-wave pulser/receiver (UTEX, UT340) and a digital storage oscilloscope (Agilent, DSOX3104A, 1 GHz, 4 analog channels) were acquired from a total of 3 sites per phantom in triplicate, first testing all specimens with the large transducers then again with the small transducers. Specimens were marked with India ink for location and accuracy of testing. The density of peaks in the ultrasonic spectra of the small transducers paralleled those of large transducers. Results from HF ultrasonic measurements of phantom breast tissue obtained from small transducers compared to the large transducers indicate that they produce statistically comparable peak densities. In breast conservations surgery it is crucial to excise all cancerous tissue to prevent recurrence. This method could provide in vivo cancer detections in margins and allow for more precise excision of tumors and cancerous tissue preventing the need for subsequent surgeries and thus, less risk, reduced pain and suffering, lower costs and better outcomes for breast cancer patients.

Mena Davidson, Westminster College Life Sciences Urban stream syndrome is the phenomenon of stream degradation as streams run from their sources through urban areas, which is exhibited by nutrient loading, decreased dissolved oxygen, changes in channel structure, and increased turbidity and rapid flow events. This can have a direct negative effect on our recreation, drinking water, and the ecosystems surrounding the streams. To discover if urban stream syndrome occurs in the Salt Lake Valley, we monitored pH, turbidity, dissolved oxygen, dissolved nitrates, and collected macroinvertebrates in three streams monthly. We analyzed total abundance, species diversity, and percent pollution sensitive species to assess the macroinvertebrate communities, as known indicators of water quality. Preliminary data shows a significant decrease in percent sensitive species in the urban areas as compared to the nonurban areas over 10 sampling periods. We found decreased biodiversity and fewer sensitive species in urban areas, although we found no difference in total abundance. These findings indicate that urbanization in the Salt Lake Valley leads to degradation in riparian ecosystems and suggest that further investigation is needed to identify the mechanisms leading to this degradation.

Teresa St. Pierre Nufer, Brigham Young University Life Sciences Acute stress has been shown to decrease Long-Term Potentiation (LTP) in the CA1 region of the mouse hippocampus. Stressed animals also show signs of anxiety and suffer decreases in spatial memory tasks such as object recognition and maze navigation. Conversely, exercise has been shown to increase spatial memory task performance in mice, attenuate anxiety-like behaviors and enhance neurogenesis and LTP in the dentate-gyrus. While the effects of stress and exercise have been examined independently, there is currently a lack of experimental evidence that connects how stress and exercise, when experienced by the same animal, might modulate LTP in the CA1 region of the hippocampus. In our ongoing study, mice have been separated into a control group, a stress group (restraint and tail-shock), and an exercise with stress group where mice have voluntary access to a running wheel (for 30 days) before undergoing the stress protocol.

Robert Erickson, Utah Valley University Life Sciences Although the insect fauna of the Colorado Plateau region are somewhat well known, our specific understanding of the arthropod biodiversity in Capitol Reef National Park is sparse.

Caitlin Carter, Utah Valley University Life Sciences High-frequency (HF) ultrasound (20-80 MHz) has been previously used to detect differences in microstructures and cell materials of different breast tissues types. These differences were used to distinguish between benign and malignant pathologies in different breast tissues. This same technology is predicted to be able to improve methods of detecting changes in cellular activity before changes in pathology take place. The purpose of this study was to use HF ultrasound to detect changes in the actin cytoskeleton, extracellular matrix (ECM), and integrin signaling, therefore differentiating the molecular subtypes associated with these changes in cell biomechanical properties. It is predicted that these cellular changes will also be associated with changes in the ultrasonic properties of breast cancer cells. The ability to rapidly and inexpensively detect the genetic changes or molecular subtypes of breast cancer would greatly impact and personalize patient treatment as well as provide more precise surgical removal of malignant and premalignant tissue. In order to test this hypothesis, four different breast cancer molecular subtypes including luminal A, luminal B, Her2+, and basal (triple negative) were grown as monolayer cell cultures. These subtypes were chosen because of their range of aggressiveness (luminal A as least aggressive and basal as most). After growth at different seeding levels, cell cultures were tested with a HF ultrasound system using a 50 MHz, 6.35-mm diameter immersion transducer and pulse-echo transmission. The data was compared to simulations using multipole expansions which predicted ultrasonic scattering based on possible variations in the biomechanical properties of malignant cells. The analyzed data showed differences in the spectra of waveform signals between each tested breast cancer molecular subtype. It is anticipated that this technique would provide an efficient and cost-effective method for differentiating between different molecular subtypes of breast cancer.

Kurt Williams, Brigham Young University Life Sciences Macrophages play an important role in innate and adaptive immune responses, inflammation, and tissue repair and are characterized by two distinct phenotypes: classically-activated (M1) and alternatively-activated (M2) macrophages. M1 macrophages are characterized by a pro-inflammatory phenotype and are involved in production of pro-inflammatory cytokines and aggressive engulfment, whereas M2 macrophages are characterized by an anti-inflammatory phenotype and are involved in production of anti-inflammatory cytokines (e.g. IL-10) and tissue repair. Macrophage engulfment of apoptotic cells leads to polarization toward the M2 phenotype and is thus “immunologically silent”. Additionally, there is evidence that tumor-associated macrophages (TAMs) tend toward an M2 phenotype and as a result offer protection from an immune response in the tumor microenvironment. To further investigate the role of necrotic and apoptotic cells in regulating macrophage polarization, we cultured human macrophages with necrotic, apoptotic, or standard viable Raji cells and fluorescent beads and performed an engulfment assay. In a preliminary study we found that macrophages cultured with apoptotic cells showed a decrease in engulfment levels compared to macrophages cultured with necrotic cells. Macrophages cultured with standard viable Raji cells had the lowest levels of engulfment compared to macrophages cultured with apoptotic cells or necrotic cells. Thus, in our initial experiments macrophages cultured with necrotic cells appear to have a more “M1” phenotype, whereas macrophages cultured with apoptotic cells appear to have a more “M2” phenotype. Further experiments are necessary to validate this preliminary data and further characterize the capabilities of necrotic and apoptotic cells to differentially polarize macrophages. If these observations are replicated, it has potential applications in cancer biology and therapeutics, atherosclerosis, diabetes, autoimmunity, and other diseases with an inflammatory component.

Lauren Archibald, Brigham Young University Life Sciences Increased intake of selenium (Se) and soy have both been shown to reduce risk for prostate cancer, especially if these dietary treatments are combined. The purpose of this project is to determine how the timing of Se supplementation of either a low- or high-soy diet affects prostate cancer risk. [C57BL/6 X FVB] F1 TRAMP (TRansgenic Adenocarcinoma of Mouse Prostate) male mice were fed stock diets low or high in soy. Half of the mice received Se supplementation (4.0 mg Se/kg BW as Se-methylselenocysteine) by gavage 5 d/wk in a 2 X 2 factorial design. Se supplementation began at conception, 6 weeks, 12 weeks, or 18 weeks of age. The mice were then sacrificed at different stages of maturation (4, 12, 18, and 24 weeks). Our results showed that, at 12 weeks of age, urogenital tract weights, a measure of prostate proliferation and tumor volume, were significantly reduced by Se supplementation (p<0.001) and by soy (p=0.044), independent of time of dietary intervention. Histological scores of prostate cancer progression also showed a protective effect of Se supplementation (p=0.030). At this writing, statistical analysis of data from mice sacrificed at 18 weeks is in process. Data derived from 18-week mice, combined with our previous findings from 12-week animals, will allow us to chart the progress of prostate cancer in this model. In addition, results will show how dietary Se and soy may alter disease progression and how the timing of dietary intervention may determine its effects.

Claudia Gonzalez, Brigham Young University Life Sciences Cortisol has been shown to be a potential bio-marker as it discriminates between individuals with and without depression (Rush et al., 1996 and Ising et al., 2007). However, cortisol has not been used to predict variation in temperament extremes that lead to pathological behaviors in adulthood. In order to examine the relationship between cortisol and temperament extremes, data from the bio-behavioral assessment (BBA) was used. The BBA data base includes data collected from over 2,700 infant rhesus macaques located in California National Primate Research Center (CNPRC). During the BBA four blood samples per subject are obtained and later assayed for plasma cortisol levels. In this study, the plasma cortisol response levels were looked at in a holistic form encompassing all individual cortisol samples. The four points of plasma cortisol concentrations were used to extract patterns of response per subject which provided classifications for each of the monkeys. The pathological patterns of cortisol response were characterized by abnormal plasma cortisol levels in response to Dexamethasone suppression testing and adrenocorticotropin ACTH injections. The variability in plasma cortisol patterns was then compared to BBA temperament ratings of vigilance, gentle, nervousness and confidence. These results showed that 12 of the 26 possible patterns of response were significantly (p<.05) related to each of the temperament ratings of vigilance, gentleness and confidence. Thus cortisol response patterns can be used both as biomarkers for vigilance, gentleness and confidence, and as potential predictors for pathological behaviors in adulthood.

Benjamin Gann, Utah Valley University Life Sciences (E)-2,4-bis(p-hydroxyphenyl)-2-butenal (2-Butenal) was shown to inhibit various inflammatory responses by inhibiting NF-kB pathway. A pull-down assay proved 2-butenal to bind to IKKb and was proposed as an active site kinase inhibitor through molecular docking experiment. However, 2-butanal has a highly conjugated aldehyde group that makes it very unstable. Therefore, we have designed more stable 2-butenal analogues and prepared them using Heck reaction. Molecular docking experiment shows that many of them have a greater affinity to IKKb.

Jeffrey Leavitt, Utah Valley University Life Sciences Central Research Question: Heptageniidae is a large family within the order Ephemeroptera (mayflies). This family consists of over 500 described species. Recently a study was done across 200 of the species to break them up into subfamilies, and genera. The studied concluded that there are 29 genera and three subfamilies Ecdyonurinae, Heptageniinae, and Rhithrogeninae (Wang, 2004). Furthermore, Ogden et al. (2009) proposed that the families Arthropleidae and Pseudironidae were derived heptageniid lineages. The phylogenetic relationships of Heptageniidae, Arthropleidae, Pseudironidae, to other closely related families are inconclusive. We propose to study these three families and the three subfamilies of Heptageniidae in detail via molecular systematics.

Clair Bidez, Westminster College Life Sciences Changes to stream ecosystems due to urbanization are known to degrade riparian ecosystems through multiple stressors including increased erosion and sedimentation, expansion of impervious surface leading to altered flow regimes, degradation of riparian vegetation and habitat, and nutrient contamination. Ultimately, such degradation can inhibit ecosystem services such as contaminant filtration and nutrient cycling. This study examined the effects of urbanization on the function of riparian communities in three streams in the Salt Lake Valley watershed. We attempted to characterize these changes through monthly monitoring in urban and non-urban reaches of the same streams. Specifically, we measured periphyton biomass (as measured by chlorophyll-a) as a known indicator of nutrient pollution and a proxy of riparian health. In addition, we monitored water quality parameters including temperature, dissolved oxygen, and dissolved nitrate concentration. The urban reaches of the streams contained 3.6 to 9.3 times the dissolved nitrate concentration as their non-urban counterparts. Mean stream periphyton biomass was positively correlated with mean stream nitrate concentration for each reach. These findings point to urbanization as a potential source of ecosystem degradation in the Salt Lake Valley. They suggest that long-term monitoring is warranted, along with an in-depth investigation into the ultimate mechanisms responsible for the degradation.

Sara Calicchia, Utah State University Humanities Successful writing, achieved by self-regulated writers, depends not only upon focus and content, but also the writing environment, including the physical and social setting, which varies greatly among writers. Just as musical tastes differ among individuals, there are strong preferences regarding the role of music in a writing setting. To better understand the environmental self-regulation of writers, I selected a group of twelve high-achieving writers with a range of musical interests, including nine professors and three undergraduate students across three academic fields. The results suggest that musical background impacts a writer’s preferred setting, and academic writers should strongly consider this impact when establishing a successful writing environment.

Jackson Bell, Brigham Young University Humanities Since a military coup in 2009, Madagascar’s low standard of living, political instability, and weak economy have worsened. One way to improve the situation in Madagascar is to improve English skills, which are import in the tourism, exports, financial aid, and international academic collaboration sectors. For example, a knowledge of the English language will give Malagasy people access to 45 percent of the world’s scholarly research journals. I recently published a Malagasy-English bilingual dictionary which aimed to meet this need. However, the dictionary is somewhat impractical because it is limited to simple, rigid definitions. It is insufficient for a complete understanding of either Malagasy or English because real-life usage of words often deviates from basic definitions.

Tyson Amundsen, University of Utah Humanities The focus of my research was to create a database of the various people, institutions, and funding agencies that constituted the discursive community of malaria specialists in Brazil—the network responsible for producing and distributing medical knowledge there from 1850-2012. This database will be used to chart trends in Brazilian research and publishing activities, Brazilian participation in international conferences, the reception of Brazilian research abroad, and Brazilian engagement with the work of foreign researchers.

Bunny Christine Arlotti, Dixie State University Humanities The Rape and Incest National Network (RAINN) reports that over two-thirds of rapes in the United States are “completed” by someone the victim knows: “The rapist isn’t a masked stranger.” RAINN’s rape statistics contrast with Frank Norris’s depiction of rape in The Octopus: A Story of California (1901). Based on an historical event, The Mussel Slough Tragedy (ca. 1880), the novel’s main plot focuses on a group of San Joaquin Valley ranchers who band together to battle the Southern Pacific Railroad’s tyrannical land grab. But out of this epic clash between man and steel emerges a subplot centered on the rape and victimization of a young woman named Angèle Varian. This paper explores the Vanamee-Angèle subplot, examining how Norris stereotypes Angèle and blames her for her own victimization. By treating Angèle as an example of what Paula Hopkins and Kristina Brooks label the “fallen woman,” Norris attempts to arouse the reader’s sympathy, not for Angèle but for her boyfriend, Vanamee, who is also one of the rape suspects. I will support my findings by examining the following works: Stuart Burns in “The Rapists in Frank Norris’s The Octopus,” Maria Brandt in “For His Own Satisfaction: Eliminating the New Woman Figure in Mcteague,” Paula Hopkins and Kristina Brooks in “New Woman, Fallen Woman: The Crisis of Reputation in the Turn of the Century,” and Joseph McElrath in Frank Norris: A Life. Ultimately, from a feminist’s perspective, Norris’s underlying message about his rape victim says volumes about his endorsement of the Victorian perspective toward the “fallen woman”-they were not worth saving, helping, or hearing from ever again.

William Palomo, Westminster College Humanities While researchers have historically marginalized the literature of El Salvador, Alfonso Kijadurías (formerly Alfonso Quijada Urías) has earned an international reputation for his diverse and incisive poetic style. Contextualizing Kijadurías’ work within the Salvadoran literary tradition reveals the historical and political backdrop driving Kijadurías’ political stances and literary experimentation. Examination of the political, philosophical, and mystical obsessions in Kijadurías’ works demonstrates how his literary career has lived up to and outlasted La Generación Comprometida, a politically-charged literature and arts movement that revolutionized the Salvadoran arts scene during the 1950’s. His work challenges contemporary politicians and the philosophy of Deconstructionism in an attempt to guide the reader through a spiritual transformation that leads to the abandonment of the self and ultimately to freedom.

Garrett Norris, University of Utah Humanities In this abstract, I will summarize the major differences and similarities I have found between Chinese and Japanese concepts of loyalty represented in the the three classical texts I analyzed.

Jane Hise, Dixie State University Humanities Modern cultural perceptions of appropriate gendered behavior can pressure individuals into unhealthy, often self-destructive, behaviors in an attempt to attain an unachievable paradigm of gendered and sexual perfection. Social norms, however, are transitory and therefore, any attempts to achieve gendered perfection based on these norms are at best inauthentic performances, and the dangers inherent in the pursuit of such skewed perceptions of masculinity and femininity are inherently dangerous to the physical and emotional health of the individual. While we are not likely to eliminate the indoctrination of current or future generations into the culturally sanctioned roles of gender performance entirely, recognizing the artifice in these expectations and behaviors can expand the boundaries of socially accepted standards to allow for healthier expressions of identity.

Megan Armknecht, Brigham Young University Humanities Transcendentalist writers such as Margaret Fuller, Ralph Waldo Emerson, and Amos Bronson Alcott were highly influenced by the German Romantic philosopher, Johann Wolfgang von Goethe. Louisa May Alcott, one of the later transcendentalists, was also an admirer of Goethe’s work and philosophy. Although considerable research has been done on the influence of Goethe on Alcott’s later works, such as A Long and Fatal Love Chase, A Modern Mephistopheles, and “The Freak of a Genius”, there has been much less research done on the Goethean influence on Little Women, and none at all on the resonances between Jo’s love interest, the German professor Dr. Bhaer, and Goethe. This research will explore the connection between Louisa May Alcott, Dr. Bhaer, and the Goethean ideal as developed in Alcott’s writing. In my presentation, I will show the importance of this connection as it adds significant depth to Little Women.

Steven Hepworth, Weber State University Humanities Mormons and Missourians were at war with one another 1838. At the heart of the issue was the political and literal power of the Mormons in the state. The Mormon leader, Joseph Smith, claimed to have received revelations stating Missouri, and more particularly Jackson County, was to be the land of inheritance for Mormon Saints. Missourians feared a Mormon overtake of the State. During this same time the growing issue of slavery was the issue in Missouri. Mormons and Missourians found themselves on opposite ends of the slavery debate. These heated issues caused both Mormons and Missourians to persecute, harass, destroy, and fight one another. Orson Hyde changed the landscape of this conflict. On October 24, 1838 Orson Hyde signed a sworn affidavit declaring that Joseph Smith and the Mormon Church planned to overtake, possess, and control the State of Missouri. At the time, Orson was an Apostle, or leading member of the Mormon Church. He was well respected within the church and throughout the Missouri community. His testimony against the church and its leaders came as a shock to many. He showed no sign of discontentment previous to his signing a sworn affidavit against Joseph Smith and the Mormon Church. Why would a leading member of the Church of Jesus Christ of Latter day saints testify against his religion, which he still held dear, and his friends? Orson Hyde indicted Mormon leaders of treason to save the lives of his family, to placate non-Mormon neighbors, and because of a failed mind due to illness. I will explore the involvement of Hyde in the Mormon Missouri war, what led him to testify against the Mormon Church, and the consequences resulted from his testimony.

Colby Townsend, University of Utah Humanities A vast amount of literature has been produced on the Book of Mormon since its initial publication in 1830. Writers from all backgrounds have approached this text, generally in a polarized manner. Either they approach it as committed believers or non-committed skeptics. In reviewing this literature it becomes apparent that not enough work has been done on the influence of the King James Version (KJV) of the Biblical text on the actual production of the Book of Mormon. A large project is underway to locate all of the places in the text of the Book of Mormon that are literarily dependent on the KJV. This paper will be a contribution toward that end. Malachi 3-4 is quoted in numerous places throughout the Book of Mormon, not just 3 Ne. 24-25 where Jesus gives the Nephites these chapters because “they [had them] not.” The paper will locate all of those places in the text of the Book of Mormon where the language and phraseology of Malachi is used, and its use will be analyzed through literary and source criticism. At present this paper is still a work in progress, and therefore has no set conclusion, but the working hypothesis is that Joseph Smith either utilized the KJV Malachi directly in the process of dictating the Book of Mormon by taking out a KJV and having his scribe copy it down, or the language was so familiar to him it came to mind as he dictated to his scribe. This will contribute to the larger study of locating all those places in the Book of Mormon that are dependent on the Bible, which will be published as a scholarly reference for use in comparative studies between the Bible and the Book of Mormon.

Echo Smith, University of Utah Humanities Since the publication, in 1995, of Jonathan Shay’s Achilles in Vietnam, an increasingly popular view of PTSD in soldiers holds that modern day combatants experience the horrors of warfare in much the same way as did ancient Greek and Roman soldiers and that PTSD must have been just as prevalent in the classical world as it is today.

Emily Simmons, Utah Valley University Humanities In Franz Kafka’s novel The Trial, the protagonist Josef K. is accused and ultimately punished for an unspecified crime of which he proclaims himself innocent. This paper examines Kafka’s novel through a religious studies approach—specifically, offering a reading of the Judeo-Christian myth of the fall of Adam and Eve, as exemplified in The Trial. I thus posit that K. is a type of post-fall Everyman who suffers the effects of the fall, attempts to restore himself to a Edenic state, but rejects the opportunity to be the fallen world’s savior and instead resigns humankind to continued suffering. To substantiate my argument I analyze four aspects of the novel. First, I examine K.’s conversation with the priest as they discuss the nature and origin of K.’s guilt. Next, I show that K.’s compulsive sexual behavior is his attempt to attain a spiritual completeness through sexual intercourse. Then, I find that as K. is suffering the effects of Adam’s fall, so too are the women suffering from the curse of Eve. Finally, I explore instances where K. exhibits similarities to the Biblical Jesus Christ, arguing that K. has the chance to perform a “savior” role but refuses. In addition to my analysis of the primary text, this paper also draws on contemporary scholars who discuss themes both Biblical and secular with an eye toward understanding K. and his relationship with women and the world around him. As a result of this analysis, I conclude that although K. is demoralized from the corrupt judicial system, maze of pointless bureaucracy, and hollow authority of the novel’s priest, he continues to seek, until the very minute of his death, salvation from the effects of the fall.

Romy Franks, Brigham Young University Humanities Following the Second World War, the adventurous westerns of 19th-century author Karl May resurfaced in Germany as wildly popular pulp fiction and dime novels. In subsequent decades, May’s literature inspired several successful film adaptations in the West, followed by alternative “Indian films” in the East. Karl May and the contemporary phenomenon of the global Western film genre tapped into many themes central to Germany’s long, tortured quest for a national identity. Post-war Germans looked to Westerns as a means for “projecting” a new image of Germany to the world-one that could help overcome the stigma of Nazism.

Jacob Newman, Brigham Young University Humanities In ‘A New Approach to Assessing Strategic Learning: The Case of Self-Regulation in Vocabulary Acquisition’ Tseng et al. (2006) discuss their design of an instrument that measures learners’ self-regulatory capacity rather than use of specific learning strategies. Learning strategies include a variety of behaviors or activities that a learner does to help them during the learning process. This instrument (SRCvoc), designed as a questionnaire, aims to help learners discover their self-regulatory capacity and then apply personalized learning strategies that are beneficial to their own vocabulary learning. Vocabulary learning is essential in developing language skills. According to their research, SRCvoc ‘can serve as a diagnostic measure to identify and understand learners’ strengths and weaknesses in terms of the five areas of self-regulation in the area of (English vocabulary) learning’ (Tseng et al. 2006: 96). The goals of the study, per Tseng et al. (2006) were (a) to create an instrument that ‘measured learner trait of self-regulatory capacity rather than survey specific behavioral habits’ (b) to create ‘an instrument based on a theoretical construct.’ SRCvoc is based on self-regulatory strategy research from the area of educational psychology, with facets including commitment, metacognitive, satiation, emotion, and environmental control and (c) to design an instrument “in one particular learning domain only, that is vocabulary learning.” We replicated the administration of SRCvoc to verify that it is of value beyond the original context. With assistance from Dr. Neil Anderson and Dr. James Hartshorn, I administered the questionnaire through a Qualtrics survey to learners in the academic program at Brigham Young University’s English Language Center (ELC) to replicate the original administration of SRCvoc. I created and distributed self-regulatory profiles from the results of SRCvoc to the students. We examined the validity and reliability of SRCvoc through statistical analysis and learned more about training self-regulated learners.

Lauren Harding, Utah Valley University Humanities From the Classical world, the male nude has come to be one of the most recognizable symbols for Greek civilization. This nude is a protean and multifaceted entity that took on different meanings as Hellenic society progressed. Two binary forms of these nudes recur as archetypes, which in this paper are identified as (1) the Heroic/Athletic nude and (2) the Ephebic/Mythological nude. Within the new discursive framework of these two physiques, it is necessary to incorporate the recent research that has been produced after the large feminist and gender studies awakening that has swept through the Classical scholarly world. The field has been wonderfully enriched with this research, and as such a re-evaluation of the male form is in order. This investigation will reveal new and fascinating aspects of Greek sculpture that previously have not been studied, and will illustrate how the distinctions between the two nudes are more easily seen, or, in certain cases, how these distinctions are blurred. Through better understanding this complicated, omni-sexual world of Classical Greece, we will enrich the understanding of our own culture, which is more nuanced than the hetero-normative standard that many believe exists.

Quinn Mason, Brigham Young University Humanities “Law is the highest reason, implanted in nature, which commands what ought to be done and forbids the opposite,” Cicero wrote, “This reason, when firmly fixed and fully developed in the human mind, is Law… Law is intelligence, whose natural function is to command right conduct and forbid wrongdoing.” In Bronté’s well-known novel, Jane Eyre, Jane is confronted with all she desires which is the love of Mr. Rochester, who is married under peculiar circumstances to crazy Bertha Mason. She is given the option of living with Mr. Rochester outside of marriage. However, Jane states, “I adhered to principle and law … [and] scorned and crushed the insane prompting of a frenzied moment”. My research focuses the role of natural law within the complicated situation presented before Jane Eyre and how she masters her passion, rather than being a slave to her desires.

Haylee Jones, Dixie State University Humanities Cesar Chavez, who experienced poverty and exploitation by way of labor practices as an adolescent, became a man who committed himself to making a positive impact on the working conditions for immigrant farm workers in America. He left a fading footprint that others have been reluctant to follow. This paper will examine various role models like Juana Estrada Chavez, Chavez’s mother, and Dolores Huerta, Chavez’s closest associate, who were big influences in Chavez’s decision to become a leader in the civil rights movements as explained by author Kathleen Krull. Authors Susan Ferriss and Ricardo Sandoval elucidated on many of Chavez’s achievements such as the organization of the United Farm Workers (UFW), a group created to unionize immigrants, and the orchestration of nonviolent strikes and marches to expose the unfair treatment of multi-ethnic farm workers. Although Chavez persuaded California to sign contracts that discriminated against biased pay and working conditions, many immigrants still work longer, more strenuous hours than the average American to receive only $2.00 an hour and live in beat down shacks they are forced to call home. Cesar Chavez had a vision that should be remembered and re harvested among Americans everywhere in order to continue improving working conditions of all citizens, no matter where they are from.

Christopher Wiltsie, Utah Valley University Humanities Low racial diversity in Utah leads to common assumptions about the lack of racial tensions. This has resulted in a general disregard for the history of civil rights struggles, specifically in education, for people of color in Utah. In the US as a whole, the 1970s featured both legal and social reform in issues of race and its role in education, but accounts from minorities in Utah tell a different story. This project will be a comparative history, analyzing oral histories regarding educational experiences of people of color that lived in Utah immediately after 1968 until 1980, within the context of the trends at the time throughout the United States. Extensive interviews will provide documentation regarding racial tensions and their effects on educational experiences and achievement. The synthesis of these accounts will help fill in the gaps that exist within Utah’s historical record. Obstacles in education for people of color during the 1970s will be discussed and compared to successes and failures throughout the United States.

Mahreen Bashir, University of Utah Humanities A complicated social paradox humanity faces is assessing the union between the seemingly contradictory ideologies of “sustainability” and “growth” into one viable system. Adapting a merger between concepts such as: the retention of cultural values and social systems, industry, urban sprawl, modernity, sanitation, progress and the environment. This objective of this research project centers around using understandings of post-colonial theory to find new connections in South Asian identity through South Asian literature written in English, and the literature’s relationship to ecology. Specifically, it applies the nascent subaltern lens of a historiography that examines South Asia as both a post-colonial subcontinent, and an emerging “superpower,” in the rhetoric of a global economy, to understand a relationship between Indian people and India as place. Questions asked are posed in regard to the affects of orientalism and a cultural infiltration of seeing endemic knowledge to be degenerate in relation to Western academia; Indian-American identity; and the weight of the term “subaltern” through works by South Asian writers. Furthermore, the project uses an ecocritical lens on these works to extract how identity discourse and post-colonialism have created understandings of Indian identity within English literature. The anticipated outcome of this research paper is to create a greater understanding of both emergent academic lenses, and their application in understanding Indian culture and identity as portrayed by works written by Indo-American writers. This research idea was born out of my passion for both ecology and post-colonial studies that I have honed through my studies as an environmental studies and gender studies double major. The main objective is to investigate the plurality of identity, and the ramifications that identity, has on the establishment of more sustainable behavior to counter the impending global crisis the planet is facing in terms of lack of environmental stewardship, and subsequent social justice.

Helen Makhdoumian, Westminster College Humanities Despite roughly a century of Armenian American literary production, the literary theory and criticism on this body of ethnic literature has only started to develop in the last few decades. David Kherdian is an Armenian American writer whose works range from poetry to prose, fiction to memoir, and translations to retellings of Armenian tales. My research focuses on David Kherdian’s poetry collection Homage to Adana, published in 1970. Previous critics have analyzed Kherdian’s poetry for themes such as childhood, familial relationships, self-discovery, and personal and collective memory. Some critics have read some of Kherdian’s poems as reflecting the loss of Armenian culture due to assimilation in the U.S. In contrast, this presentation analyzes the motif of the Armenian coffee house in select poems in Homage to Adana as a space where geographical, generational, and cultural identities are negotiated. Although on the surface these poems indicate a loss of Armenian culture, I argue that they actually indicate a continuation and adaptation of Armenian culture in the U.S. by the younger generation. To support my argument, my approach uses the theoretical lenses of New Historicism and Cultural Studies. I contextualize these texts with the history of the Armenian diaspora and immigration. Furthermore, I include the historical and cultural significance of Armenian coffee houses as well as the use of Armenian coffee in cultural knowledge sharing. In general, the themes I look for are references to the old country, representations of immigrant Armenian men and women, oral storytelling, and food traditions. Ultimately, this analysis reveals how the poems reflect the negotiation of passing on cultural knowledge. By both continuing traditions and adapting them for everyday lived experiences, Armenian culture will remain vibrant in diaspora.

Laurin Wilson, Utah State University Social and Behavioral Sciences Premature birth and subsequent hospitalization of an infant in the Newborn (or Neonatal) Intensive Care Unit (NICU) can be an extremely stressful time for parents and has been associated with maternal depression, anxiety, and decreased coping ability (Hack, Taylor, Klein, & Mercuri-Minich, 2000; Hughes, McCollum, & Sheftel, 1994; Partridge et al., 2005; Shaw, Sweester, St. John, Lilo, Corcoran, Jo, & Horwitz, 2013). Interventions to reduce parents’ stress levels during the hospitalization of their very preterm infants have mixed results (Boyce, et al., 2008; Matricardi, S., Agostino, R., Fedeli, C., & Montirosso, R., 2013). This study examined the medical, family, and maternal mental health factors that influenced feelings of parental distress for mother of very premature infants.

Dusty Pilkington, Weber State University Social and Behavioral Sciences Boundaries dividing political authority rarely match natural water flow. Multiple agencies, often with competing policies and agendas, manage water within single watersheds. This discrepancy can render management efforts ineffective. Local water agencies include soil conservation districts, local health departments, and engineering agencies. Agencies have varying funding sources, managerial structures, and jurisdictional boundaries. Fragmentation can frustrate management efforts at watershed scales. Comparisons between natural watersheds and human political geography can therefore be helpful in avoiding conflicts. This research examines discrepancies between political and physical geographies for sections of three Utah rivers : the Logan and Provo Rivers, and Red Butte Creek. These rivers are being targeted for intensive research as part of a statewide, multidisciplinary water sustainability project, titled iUTAH. Water-related land use (WRLU) data acquired from Utah’s Automated Geographic Resource Center were analyzed using ArcGIS geoproceessing tools. WRLU contributing to water quality in urban river reaches is described. WRLU was examined using three boundary sets, comparing the political geography of the river reaches, as depicted in municipal boundaries and management areas of community water providers, to their physical geography, as depicted in United States Geologic Survey Hydrologic Unit Code (HUC) watersheds. WRLU mixes using political infrastructure were contrasted with land use mixes from HUC boundaries. WRLU was classified in seven categories: residential, commercial/industrial, riparian/water features, irrigated agriculture, non-irrigated agriculture, farmsteads, and parks/open spaces. A gradient from rural Heber, to urbanizing Logan, to fully urbanized Red Butte Creek is shown. While Red Butte Creek HUC boundaries showed 53.8 % residential land use, Red Butte Creek municipal boundaries and community providers totaled 26 % and 39 % residential use, respectively. Differences emerged when irrigated agriculture was assessed using HUC boundaries. Irrigated agriculture totaled 0.2 % in Red Butte Creek, with Logan showing 29. 7% and Heber irrigated agriculture sitting at 41.1 %.

Luz Maria Carreno, Utah State University Social and Behavioral Sciences Significance: Patients’ health literacy, or ability to comprehend and understand health information, influences their health status, knowledge about medical care and conditions, and hospitalization and adherence rate (Andrus, & Roth, 2002). Low-English proficiency patients are especially at risk, given they must overcome English and health literacy barriers.

Kelsea Hill, Utah Valley University Social and Behavioral Sciences Sexuality has long been identified as foundational in the lives of married couples. An area lacking in research, however, is the transition into sexuality made by couples who practiced abstinence prior to being married. The data for this study comes from a survey of 597 respondents that had practiced abstinence before being married. This focuses on the qualitative responses to questions asked abstinent couples about what helped make the transition into sexual intimacy, what purpose(s) sex has in their relationship, what they wished they had discussed prior to having sex, and what challenges they experienced in the transition into sexual intimacy.

Jared Glenn, Utah State University Social and Behavioral Sciences Previous research shows that girls and women tend to prefer and perform better in classes taught by women. However, much less research analyzes the impact of instructor gender on learning outcomes among boys and men. This research fills that gap by analyzing the effect of professor gender on male college students’ achievement. Relying on survey and in-depth interviews among undergraduate men at a large research university, this study finds no significant empirical data that professor gender significantly impacts men’s academic achievement, in contrast to previous findings among girls and women. Interview findings, however, suggest that men prefer the tough grading, which they indicate is integral in male-taught classes. The study contributes to our understanding of gender differences in an academic setting and how that factor shapes learning outcomes and achievement.

Rachel LaForce, Brigham Young University Social and Behavioral Sciences On an island of 65,000 people, Guernsey’s Housing Control Law offers a two-tiered alternative to the free market system. In an attempt to more closely regulate the growing population of their limited area (approximately 25 square miles), the States of Guernsey implemented the Housing Control Law in the late 1940s. With a provision that allows it to be altered and updated every 10 years, there is a distinct change over time in its original purpose and its contemporary companion. This study is based on information gained by using ethnographic methods during a two month field study in Guernsey. Questions focused on how residents perceived the purpose of the law, and how they believe it achieved those purposes. Results suggest that residents accurately perceive the original purpose of the Housing Control Law as described by the States of Guernsey, hoping to preserve the majority of housing opportunities for the indigenous population. Although their view of the modern implementation is much more varied, most point to the benefits that attracting high net-worth individuals brings to the island’s economy and also justify the use of housing incentives for skilled individuals to fill a labor shortage. Resident perception aligns with the original purpose of the Housing Control Law. With change over time, Guernsey residents understand the necessity to increase economic activity and fill labor shortages which can be provided via housing incentives, especially a two-tiered housing market.