2014 Abstracts

Audrey Butler, Utah Valley University Life Sciences High-frequency (HF) ultrasound has been shown to be sensitive to a range of breast pathologies, and is being explored for the intra-operative assessment of lumpectomy margins. This sensitivity is believed to arise from microstructure-dependent interactions of ultrasound in the tissue. The objectives of this study were to develop breast tissue phantoms with microstructures that accurately mimic the histology of normal and malignant tissue, and to determine the effects of these microstructures on HF ultrasonic spectra (10-100 MHz). Phantoms were created from a mixture of water, gelatin, and soluble fiber. To simulate various breast tissue histologies, polyethylene beads, polyethylene fibers, and nylon fibers with a range of diameters were embedded into phantoms. Microstructures ranging from randomly dispersed beads to bead-fiber constructs resembling terminal ductal lobular units (TDLUs) were modeled and tested. Pitch-catch and pulse-echo measurements were acquired using 50-MHz transducers, a HF pulser-receiver, and a 1-GHz digital oscilloscope. Spectra were derived from the data and peak densities were determined from the spectra. Peak density, which is the number of peaks and valleys in a specified spectral range, has been shown to correlate with tissue complexity. Preliminary results from dispersed beads (58-925 µm diameter) of constant volume concentration (0.8%) indicated that the smaller beads produced higher peak densities than the larger beads with a consistent and statistically significant trend. These results substantially improve upon previous phantom studies and upon results from original breast cancer studies, demonstrating the strength of the HF ultrasound response to tissue microstructure. The higher peak densities can be attributed to either the higher number of scatterers for small beads or the size of scatterer in relation to the ultrasonic wavelength. These and other results from more advanced histologically accurate microstructures modeling TDLUs will be discussed.

Josh Harvey, Brigham Young University Life Sciences Soil CO2 flux represents an important pathway of carbon transfer from ecosystems to the atmosphere. Soil CO2 flux can be altered by global warming-driven changes in seasonal temperature and water availability. Subalpine ecosystems have high levels of carbon in their soils that are stabilized by low temperatures and low microbial activity during long and snowy winter seasons. Subalpine ecosystems can be important sinks for carbon, storing carbon that otherwise would be in the atmosphere contributing to global warming. In our study we show how changes in temperature and water availability during springtime increase the levels of subalpine carbon output. So long as the carbon outputs outweigh carbon inputs, increases in soil flux would amplify global warming. The amplification of global warming would loop back to affect soil fluxes again (by raising temperatures, melting snow earlier, and changing precipitation patterns) thus creating a positive feedback system. Understanding what feedbacks are present in a climate system and their underlying mechanisms will improve our forecasts of changes in atmosphere chemistry and temperature.

Logan Warner, Utah Valley University Life Sciences High-frequency ultrasound (20-80 MHz) has been found to be sensitive to a range of pathologies in excised breast tissue before fixation in formalin or other formaldehyde analogues. Formalin fixation, however, may alter the structure and rigidity of a sample so that data gathered using high-frequency ultrasound after fixation may no longer be viable for research purposes. This limits the amount of time researchers may conduct tests, so preservation via simple sugars is being considered. Numerous studies have been conducted using sucrose, trehalose, or glucose as cryoprotectants for cells and simple tissues. The objective of this study was to test the sensitivity of high-frequency ultrasound to changes in the microstructure, stiffness, and cellular integrity of tissue samples due to cryopreservation with these sugars. Domestic pig heart tissue was placed in aqueous solutions of sucrose, trehalose, and D-(+)-glucose. The specimens were refrigerated and observed over time using high-frequency ultrasound to detect tissue damage. The results of this study suggest that cryopreservation with sugars will not only allow more time for researchers to conduct ultrasonic tests on surgical specimens, but also that high-frequency ultrasound could potentially be used as an assay to measure tissue degradation in preserved living tissues such as transplant organs.

Hillary Hansen, University of Utah Life Sciences Type 1 diabetes is an autoimmune disease where pancreatic β-cells are destroyed, resulting in insulin deficiency. Generating new β-cells from stem cells for treating diabetes will benefit from understanding their development in vivo. Pancreatic β-cells, along with all other pancreatic lineages arise from multipotent pancreatic progenitor cells (MPCs). Previous studies demonstrate that the structural and signaling protein β-catenin is required for the development of the exocrine acinar lineage. β-cells still differentiate in the absence of β-catenin, however, β-cell mass is dependent upon β-catenin. We determined that this dependency reflects a role for β-catenin in the maintenance of MPC patterning as well as for expansion of the progenitor pool. Whether our observed effects are due to the signaling or structural function of β-catenin remains unknown, and is the focus of this research. Using mouse genetics we are able to separate the structural and signaling functions of β-catenin. Eliminating both functions in PBKO (full knockout) mice produces decreased β-cell mass and irregular patterning. Decreased β-cell mass is also observed in PBsKO (signaling deficient) mice, though patterning remains unaffected. This suggests that pancreas growth is dependent upon canonical Wnt/β-catenin signaling, and that maintaining progenitor identity requires the structural role of β-catenin. Elucidating distinct roles for β-catenin could be used to drive stem cell-derived MPCs to expand and differentiate to the desired pancreatic cell fate.

Michael Naegle, Brigham Young University Life Sciences Dermaptera is a comparatively small order of insects with approximately 1800 species placed in three suborders. While the majority of earwig species are placed within the suborder Forficulina and are free-living with forceps-like appendages, two dermapteran lineages have a very unusual morphologies and life histories. The viviparous Hemimerina live epizoically on giant rats in tropical Africa where they feed on fungi growing on the rats’ skin. Hemimerina lack eyes and wings and the cerci are filiform. The viviparous Arixenina are associated with bats in Malaysia and the Phillippines, and they feed on bat skin gland secretions. They have reduced eyes, are wingless, and possess straight cerci. The phylogenetic position of the suborders Arixenina and Hemimerina relative to Forficulina have previously been unclear; however preliminary analysis suggest the phylogenetic position of the suborders Arixenina and Hemimerina are nested within Forficulina, with ectoparasitism evolving multiple times within this order. We generated DNA sequence data from three nuclear (18S, 28S and H3) and two mitochondrial (COI and TUBA) genes for representatives of all three suborders and outgroups. A phylogeny was reconstructed to address the following questions: (1) Does Hemimerina + Arixenina form a monophyletic group and support a single origin of parasitism or are there multiple origins of parasitism? (2) Is Forficulina monophyletic with respect to these parasitic lineages? (3) Are morphological similarities shared by the ectoparasitic forms synapomorphic or homoplasious characters?

John Hancock, Brigham Young University Life Sciences LLO56 and LLO118 are CD4+ T cells specific for the same Listeria monocytogenes epitope. Despite their TCRs differing by only 15 amino acids, LLO118 and LLO56 have dramatically different primary and secondary responses to Listeria monocytogenes infection. We reasoned that LLO56, the single chain TCR (Vβ2-linker-Vα2) could be subjected to directed evolution to generate mutants that are more stable and bind to peptide-MHC with higher affinity. Single chain LLO56 was fused to the yeast surface protein Aga-2 and error prone PCR was used to generate mutagenic libraries. A first generation stabilized single chain TCR (scTCR) was selected using biotinylated Vβ2 and Vα2 antibodies and anti-biotin beads. The first generation LLO56 mutant expressed LLO56 on the surface of yeast at higher levels than wild type by flow cytometry. To produce mutants with additional stability, a second-generation mutant was generated by combining multiple stability mutations isolated in a number of first generation clones.

Brian Ballard, Brigham Young University Life Sciences Antigen presenting cells digest and display proteins from foreign and infected cells on the major histocompatibility complex (MHC) which can then be recognized by T-cells through their T cell receptor (TCR). LLO56 and LLO118 are CD4+ T cells specific for the same Listeria monocytogenes epitope but show dramatically different primary and secondary responses to infection. Because TCRs have very low affinity for MHC we would like to create a high affinity T cell. We reasoned that the single chain LLO118 TCR (Vβ2-linker-Vα2) could be subjected to directed evolution to generate mutants that are more stable and then used as a template for engineering high affinity T cell receptors. Single chain LLO118 was fused to the yeast surface protein Aga-2 and error prone PCR was used to generate mutagenic libraries. The first generation stabilized LLO118 single chain TCR (scTCR) was selected using biotinylated Vβ2 and Vα2 antibodies and anti-biotin beads and it expressed LLO118 at higher levels than wild type by flow cytometry. To produce mutants with additional stability, a second mutagenic library using the first generation mutants as templates has been produced and the most stable clones will be selected after temperature denaturation, permitting isolation of clones with increased stability for generating high affinity pathogen specific scTCRs. After engineering a high affinity T cell our research will further understanding on TCRs and the MHC and could also serve as a resource for creating a therapeutic drug.

Lance Deeter, University of Utah Life Sciences Continuous-flow left ventricular assist devices (LVADs) are used in advanced heart failure patients either to bridge them to transplantation or as a permanent-destination therapy. We determined whether chronic exposure to non-pulsatile blood flow and acute increases in coronary perfusion pressure associated with LVAD implantation would influence arterial function. Arteries from a transmural biopsy of the left-ventricle were obtained from ten male patients (54±4 years old) at the time of LVAD implant (n=17, 184±25 µm i.d.) and 239±51 days later upon LVAD explant (n=21, 281±22 µm i.d.). Lmax tension was determined and dose-response curves to potassium chloride (KCl, 10-100 mM) were performed using isometric tension techniques. Next, bradykinin (BK, 10-6 to 10-10 M) and sodium nitroprusside (SNP, 10-4 to 10-9 M) concentration-response curves were completed on vessels precontracted to ~65% of maximal tension development. Maximal BK-induced vasorelaxation was greater (p<0.05) at explant (85±5%) vs. implant (59±9%), while SNP evoked responses (~90%) were similar between time-points. These findings suggest coronary endothelial function is improved by LVAD implantation. Heart failure was precipitated by a myocardial infarction in six of the ten patients. These are referred to as “ischemic” patients whereas the remaining four are “non-ischemic” patients. We hypothesized that coronary vascular responses would be improved by LVAD implantation to a greater extent in ischemic vs. non-ischemic patients. In ischemic patients maximal BK-induced vasorelaxation was greater (p<0.05) in coronary arteries obtained at explant (87±6%, n=14, 305±30 μm i.d.) vs. implant (53±11%, n=12, 204±33 μm i.d.). In non-ischemic patients maximal BK-induced vasorelaxation was similar in arteries obtained at explant (79±9%, n=7, 232±21 μm) and implant (72±17%, n=5, 135±13 μm). SNP responses were similar (~90%) between groups at implant and explant. Collectively, our data suggest that LVAD implantation improves endothelium-dependent vasorelaxation in ischemic but not in non-ischemic patients.

Ashtyn Smith, Westminster College Life Sciences Methylmercury (CH3Hg) is a neurotoxin that accumulates in lakes and streams due to the action of microorganisms, which can produce this biologically relevant organic form from elemental mercury (Hg). Therefore, the activities of microorganisms become key to understanding the balance of Hg and CH3Hg in the movement through the food chain in any ecosystem. Many species of microorganisms are resistant to Hg and can thrive in polluted waters. Recent studies have shown that Hg resistance in microbes can stem from one of two gene pairs, merAB or hgcAB. The merAB system allows the organism to covert CH3Hg into elemental Hg. Conversely, the hgcAB system coverts Hg into CH3Hg. Thus, it is important to determine how the microbial community of Great Salt Lake, Utah is affecting the CH3Hg concentrations in the lake. In order to determine the genotype of the lake’s halophiles, “salt-loving” organisms, microorganisms were collected from the deep brine layer in eight areas of the lake. The microorganisms were then isolated and cultivated on increasing concentrations of HgCl2. Halophiles from these samples have been isolated on 25 ppm HgCl2 at various salinities, demonstrating a robust resistance to Hg. PCR amplification and genetic sequencing will be used to determine the gene mechanism of mercury resistance (merAB or hgcAB) as well as the 16S rRNA gene, which will aid in identification of the species. Should this study identify GSL microorganisms that exhibit the merAB genotype, these organisms could potentially be utilized as bioremediators of the CH3Hg pollution in the lake.

Dusting Day, Brigham Young University Life Science Atherosclerotic vascular disease is the leading cause of morbidity and death in the United States. Approximately 1.4 million surgical procedures are required every year for treatment of vascular disease and its subsequent issues. While saphenous vein and internal mammary artery grafts are most commonly chosen by physicians, many patients who are in need of arterial grafts have vessels that are not ideal for grafting because of damage to the vessels or disease. This introduces the necessity for synthetic blood vessel grafts that function precisely as natural vessels in vivo. Our blood vessel research team has entered the tissue engineering field in its most exciting effort: the scalable rendering of cell-seeded vascular constructs with rapid prototyping machines or 3D printers. We have built and are modifying a 3D printer to deposit living endothelial and smooth muscle cells into vascular structures. Using agar, alginate, or collagen gels as placement media, cells can be arranged in shapes resembling multilayered artery tubules and proliferate to form functional arteries. The endothelial layer and smooth muscle layer of cells interact to secrete a natural extracellular matrix (ECM) between them. We have successfully cultured endothelial cells and are perfecting our technique of harvesting aortic smooth muscle cells for culture. These cells will be encapsulated in a gel we have optimized for cell adhesion and proliferation and will then be printed with our rapid prototyping machine into the shape of a blood vessel. After proper cell growth and secretion of the ECM we will subject our synthetic graft to tensile strength testing, thrombosis tests, and eventually implantation into an animal for observation of any immunogenic effects. Our project’s success would bring an array of new treatment options through biomedical engineering that would save many lives of those who suffer from cardiovascular disease.

David Money, Brigham Young University Life Sciences Interest in animal personalities, and particularly the effect that different environments have on personality, has increased dramatically over the past decade. Understanding how individuals vary in their behavior, and if there are consistent differences among populations from divergent selective environments, lays the foundation for studies focusing on the contribution of divergent behavior in species formation. To date, studies that have focused on how personalities differ across ontogenetic stages have failed to compare populations that occur in dramatically different environments. Our study attempts to fill this void by studying the ontogeny of personality in populations that have evolved in environments with different levels of risk (i.e., predation vs. no-predation). We tested the expression of different personality traits evolution across ontogeny (i.e., from juveniles to full grown adults) in two sister species of live-bearing tropical fish, Brachyraphis roseni and B. terrabensis. These species have evolved in different selective environments, with B. roseni having evolved in an environment where predators were present, while B. terrabensis evolved in an environment lacking predators. We assessed the boldness expression of individuals from several groups in populations, namely juveniles, small adults, and large adults. To measure boldness, we used an emergence test, and also an exploration and activity test (i.e., ratio of movement to idleness during an allotted time period). Our study provides evidence for an important relationship between predation environment and the evolution of personality traits across ontogeny.

Colby Pearson, Utah Valley University Life Sciences Many lichens synthesize unique secondary metabolites, such as atranorin (AT), which may serve as photo-protection against harmful UV radiation. Our study investigates changes in metabolism and internal levels of atranorin in lichens under various light conditions and atranorin supplementation. We will expose 60 Physcia adscendens (Fr.) H. Olivier lichen thalli to one of three different light levels (UV +PAR; PAR only; and neither UV nor PAR). Half of our experimental thalli will be supplemented with AT. Lichen biomass, AT concentration via HPLC, and photosynthetic and respiration rates will be measured before and after the experiment to measure metabolic responses of both supplemented and non-AT-supplemented thalli under each light level.

David Vogelsang, Brigham Young University Life Sciences More than one-third of U.S. adults (35.7%) are obese (CDC, 2013a), and since 1980, obesity among adolescents has risen from 5% to 18% (CDC, 2013b). Unless we do something to combat the growing obesity epidemic, we are consigning ourselves and future generations to a lifetime of heart disease, diabetes, cancer, and psychological distress (CDC, 2013a). Cooking Anatomy Academy (CAA) promotes healthy eating and cooking among parents and students in the Polynesian community to raise awareness about the growing obesity epidemic. Our primary focus is introducing parents and students to healthy, great tasting foods and easy to prepare meals (Brown, 2011). Our secondary focus is to teach the simple anatomy and physiology important to understanding healthy nutrition. CAA is composed of seven, one hour lessons that are being offered as an afterschool program at Mana Academy Charter School. We’ve developed the CAA curriculum to incorporate many of the nutrition guidelines on MyPlate.gov, and focus on moderation, variety and raw/unrefined foods. To study the impact of Cooking Anatomy Academy, we will take a qualitative approach and use journal entries to collect data. Participant journal entries will answer prompts like, “based on what you learned today, what will you have for a snack tomorrow,” or, “how many fruit servings should you have each day?” CAA mentors will record any positive or negative feedback they receive during each lesson. From the data we collect, we hope to see that CAA is helping participants make healthy food choices and increasing their obesity awareness.

Derek Harris, Dixie State University Life Sciences The presence of extracellular DNA (eDNA) in various environmental and biological media has become the subject of growing interest in the field of research. In media such as bacterial biofilms, it has been shown to play a vital role in their structure and antimicrobial properties. Existing methods for extraction of pure eDNA from these media are complex and problematic; particularly from biological media where cells containing genomic DNA are also present. Novel surfactants have been developed, whose miscibility and polarity are easily tuned to suit a variety of conditions necessary for eDNA extractions. They can accomplish extraction of pure eDNA through concurrent hydrophilic and hydrophobic interactions in a single step, while remaining unreactive with the surrounding media or lysing cells and exposing genomic DNA. We have shown by spectrophotometric quantification that these surfactants extract measurable amounts of DNA into a water-immiscible solvent layer, which can then be removed from the media. The DNA can then be further amplified and purified for analysis. Further refinement of extraction methods utilizing these surfactants could prove a tremendous asset to research attempting to elucidate the possible genetic content of eDNA and the mechanisms behind its often crucial role in environmental and biological media.

Austin Pearce, Brigham Young University Life Sciences Agave utahensis acts as a keystone species across its native range in the Mojave Desert and Colorado Plateau (Gentry, 1982). As a keystone species, Utah agave contributes to soil formation along barren mountain ridges, and has provided starch-rich sustenance to Native American tribes. Furthermore, taxonomists consider each of the two subspecies, kaibabensis and utahensis, to have considerable morphological variation (Gentry, 1982) within their own unique ecological niches. Given the importance of Utah agave, the high degree of variation, and its unique ecological niches, there is surprisingly little information published regarding its physiological ecology. In fact, no effort has been made to determine the population densities of Utah agave due to the remoteness of the region and its difficult terrain (e.g., the Grand Canyon). Therefore, geospatial analysis tools specific to environmental niche modeling provide a powerful means through which these issues and knowledge gaps can be effectively addressed. My goal is to develop a species distribution model by joining known locations of Utah agave with climatic and environmental data in MaxEnt and ArcGIS software. Such a model can be used by others for further ecological field studies of Utah agave and its subspecies. Additionally, the approach I employ can be used as a pattern for mapping distributions of other important plant species in remote and difficult-to-access regions of the world.

Daniel Loveland, Brigham Young University Life Sciences The monoamine oxidase A (MAOa) gene has been shown to be associated with various social behaviors and disorders such as: aggression, depression, and anxiety (Meyer et al., 2006; Kinnally et al., 2010; Newman et al., 2005); and the MAOa gene interacts with environmental influences to produce its phenotypic effects (Newman et al., 2005; Kinnally et al., 2010). The MAOa gene encodes the enzyme monoamine oxidase A, which is the main enzyme to break down the monoamines into their respective metabolites. An orthologous repeat variant of the MAOa genotype seen in humans has been found in the rhesus macaque: a 5 repeat (R), a 6R and a 7R. This study investigates the influence MAOa genotypes have on central monoamine functioning as measured by cisternal cerebrospinal fluid (CSF) monoamine metabolites associated with behavioral dysfunction (dopamine metabolite: homovanillic acid-HVA, norepinephrine metabolite: 3-methoxy-4-hydroxyphenylgycol-MHPG, and serotonin metabolite: 5-hydroxyindoleacetic acid-5-HIAA). Cisternal CSF was obtained from 136 30-day old infant male rhesus macaques with varying genotypes and rearing backgrounds. We expected to find a rearing by genotype (GxE) effect on the monoamine systems with differences between mother-reared subjects when compared to subjects reared without mothers in peer-only groups. We found significant variability between genotypes; results also showed early rearing modulated this genotypic effect on brain chemistry. This supports our hypothesis that GxE interactions influence monoamine metabolite concentrations, suggesting a possible relationship of GxE interactions on social disorders such as aggression, depression and anxiety.

Brittany Hammontree, Dixie State University Life Sciences Depending on metabolic conditions or dietary preferences, people can often become deficient in critical vitamins and minerals. For example, a number of people are deciding to become vegetarians, and vitamin B12 deficiencies could become a huge epidemic, as this essential vitamin is only obtained through meat products. This issue was the driving force to look deeper into new ionic liquid materials and how they could be used as a transport agent for vitamin B12, along with other vitamins. Ionic liquids are organic salts that are currently being explored in many scientific fields due to their unique properties. However, using ionic liquids as a transporter in transdermal applications has yet to be explored Developing new mechanisms of administering nutrients via transdermal processes can increase the bioavailability and effectiveness of vitamins and minerals that often cannot survive oral administering due to the acidity and molecular absorption via the stomach. This research focused on finding the right ionic liquid with high solubility of the individual vitamins. Several ionic liquids were developed, and the different vitamins were tested for solubility levels. This greater solubility allows for maximum exposure of the vitamin during transdermal delivery. In particular, two different vitamins were tested – vitamin K and Protoporphyrin, a chemical analog to vitamin B12. Additionally, the effect of these ionic liquids on the physiology of the blood and plasma as it enters the body past the skin layers is critical to understand. In addition to transdermal applications of vitamins, transport of these vitamins to other countries and remote locations could have tremendous implications. Ionic liquids tend to increase shelf life of solutes, and the availability to provide these materials during medical missions or service trips would be increased substantially, particularly in more remote settings.

Eric Middleton, University of Utah Life Sciences One of the most impressive examples of homing among birds is demonstrated by Columba livia, or the rock pigeon. In racing or carrier breeds, birds can routinely travel over 100 miles and reliably find their way back to their home loft (Pratt, 1954). Bred from feral rock pigeons, these racers and carriers have been selected for their increased ability to home. While much research has been conducted on the mechanisms of homing, the heritability of homing is not very well understood. Furthermore, it is unclear if homing is predominantly learned or innate (Melhorn, Haastert, Rehkamper, 2010). The existence of homing breeds demonstrates that homing is heritable, but the degree to which this is the case or what traits in particular are improved with selective breeding are more uncertain. To attempt to better understand the heritability of homing, we will compare the homing ability of pigeons who are siblings to each other, and to unrelated pigeons. We first captured 90 wild pigeons from areas around Salt Lake City and allowed them to breed freely. We tracked the ancestry of all of the pigeons born from the feral birds and banded each of the birds in order to reliably distinguish which birds were related. We are now in the process of teaching the captive bred birds to home. Once this is completed, we will release the birds at varying distances from the loft. To quantify homing ability, we will measure angle of displacement from the loft when the birds first orient themselves and being homing, the time it takes to home, and which birds successfully make it home to the loft. This data will then be used to compare the homing ability of siblings to unrelated pigeons.

Braden Hancock, Brigham Young University Engineering In Evolutionary Multi-objective Optimization (EMO), the mechanism of epsilon-dominance has received a lot of attention because of its ability to guarantee convergence near the Pareto frontier and maintain diversity among solutions at a reasonable computational cost. The main weakness of this mechanism is its inability to also identify and exploit knee regions of the Pareto frontier, which are frequently the regions of the frontier that are most interesting to the user. Many attempts have been made to resolve this issue, but each has resulted in either decreased computational efficiency or slower convergence. We therefore propose a new mechanism – Lamé-dominance – as a replacement for epsilon-dominance in EMO. The geometry of the Lamé curve naturally supports a greater concentration of solutions in directions of high tradeoff between objectives. This adaptable resolution of solutions in knee regions of the Pareto frontier will result in significant savings in time and money for complex optimization routines in large n-objective design scenarios.

Raymond Bilodeau, Brigham Young University Engineering Extreme piezoresistivity was discovered in a silicone/nickel nanostrand (silicone/NiNs) nanocomposite. A quantum mechanical tunneling percolation model has been developed which bridges the gap between quantum effects at the nanoscopic scale and bulk material response at the macroscopic scale. To further improve on this theory, a measurement technique was adapted for use in measuring the average distance (in nm) between the NiNs in the silicone matrix. The measurements produced strong correlation to newly developed theories on the nature of the nickel-silicone interaction. The predictions of the previously developed model were also compared to these new experimental measurements and the model is being adapted to more accurately represent the real data.

David Lindell, Brigham Young University Engineering Current ion detector technologies require low pressures and temperatures to achieve high sensitivity. These extra constraints result in bulky or expensive ion detection units and make a highly-portable mass spectrometer difficult or impractical to produce. A new ion detector technology that is unhampered by such constraints would allow the construction of miniaturized mass spectrometers. Such devices would have a myriad of potential applications, including use in space probes, on-site chemical weapon analyses, and in-field forensics. This research has produced solid-state ion detection devices with detection levels in the hundreds-of-ions range. The detectors are produced on a printed circuit board, are inexpensive, and are functional at room temperature and pressure. Solid-state detection capabilities were realized by adopting concepts from modern non-volatile (flash) memory and using custom-made low capacitance MOSFETs. Detection occurs as ions impact a Faraday cup and charge the gate of a MOSFET, yielding a voltage change in the circuit. In addition to refinements made by incorporating low-capacitance MOSFETs, commercial MEMS switches (which have only recently become available) are used to produce ion counts at rates up to 30 kHz. Amplification and filtering circuitry has also been added to further increase sensitivity levels. Results of this research show that ion detectors can be reduced in size and complexity, making a portable mass spectrometer more viable.

Anthony Bennett, Brigham Young University Engineering Foot Mouth Disease is considered to be the greatest hindrance to livestock trade in the world. The disease is extremely contagious and can transmit via aerosol, food scraps, and through blood, and tears among other transmission routes [1]. Currently, technological challenges hinder eradication efforts due to a wide variety of FMD strains, high vaccine production costs, as well as limited efficacy of vaccines across strains [2]. The countries most affected by the disease also face economic, social, and political challenges to disease eradication. Based upon historical evidence disease eradication has proven to be possible as shown in the US, the UK, and other countries [3]. In this presentation, we highlight these challenges and propose various routes to eradication in order to open up economic opportunities to developing countries as well as eliminating the threat of a disease outbreak in countries currently free of the disease. Morgan, E.R., et al., Assessing risks of disease transmission between wildlife and livestock: The Saiga antelope as a case study. Biological Conservation, 2006. 131(2): p. 244-254. Parida, S., Vaccination against foot-and-mouth disease virus: strategies and effectiveness. 2009. Perry, B. and K. Sones, Poverty reduction through animal health. Science, 2007. 315.

Benjamin Lindsay, Brigham Young University Engineering Current treatments for cancer and diseased tissue often cause severe side effects due to drug interactions with healthy cells. In order to minimize these effects, we are developing a nano-scale near-infrared (NIR) light-responsive drug delivery system based on liposome-encapsulated perfluoropentane (PFC5) emulsions with gold nanorods in the PFC5 phase. The nanorods efficiently convert NIR light to heat, vaporizing the liquid PFC5 emulsions, which have boiling points near body temperature. Emulsion vaporization increases the volume inside the liposome enough to burst the phospholipid bilayer and release encapsulated cargo. This system will allow continuous therapeutic drug release localized at the site of NIR laser irradiation with a low-power, portable NIR laser diode. To date, we have successfully loaded PFC5 emulsions with gold nanorods and have loaded liposomes with PFC5 emulsions. Previous work in our lab has shown that a release to the cytosol of cells can be induced by ultrasound using similar liposomes. Experiments designed to demonstrate NIR laser-induced cargo release are currently in progress. We will continue to improve upon this system over the coming months to increase release and decrease the required laser power.

Bradley Hackett, Utah Valley University Engineering The Arduino microcontroller is very robust and is capable of performing a wide variety of functions to fit the needs of almost countless different projects. Many robots that are being built use a microcontroller, and the Arduino microcontroller is a good fit for many robots. The microcontroller is also standardized so it can be learned easily, and maintained easily as well, especially in a team environment. The simplicity of the Arduino offers a much more accessible design and development environment. This environment allows for very complex projects such as robotics to be approached in a manageable way. The SERA Bot is a robot which is designed efficiently and yet still has complexity, using the Arduino microcontroller. The S.E.R.A Bot is an acronym which stands for Searching Exploring Roaming Autonomous robot. The first task this robot can do is to communicate over Bluetooth to an Android tablet or phone to remotely control the robot by driving the motors or sending command signals to the Arduino microcontroller. The next task it is capable of is to use Light Dependant Resistors or LDRs to follow a light source or find the most brightly illuminated area of a room. The third task is object avoidance which is implemented using a simple ultrasonic distance sensor to detect if there is an object close in front of the robot. The last function of this robot is the ability to locate a beacon which emits a loud detectable sound, and is implemented using three amplified condenser microphones. The purpose behind this project is mainly to incorporate many simple functions to one robot. Many robots do one simple task, but the focus is to create a versatile robot, with enough complexity to be useful, but simple enough to be efficient.

Zola Adjei, Brigham Young University Engineering The research project is aimed at developing a tool to monitor the progress of rehabilitation efforts in Lake Malheur in Harney count, Oregon. The application of remote sensing techniques, which will be used to detect chlorophyll-a distribution from water algae growth in the lake. Concentrations of chlorophyll-a act as an indicator for algal blooms, which compete for nutrients and oxygen and can have significant detrimental effects on a body of water. To better identify the trend in growth activities of algal colonies, remote sensing will be effective in developing a model to map the path and region of high activities of algal growth and subsequently monitoring fish habitation on the entire Malheur Lake. The method uses satellite images which measure the reflectance of pigment concentrations, which can then be quantified as concentrations of chlorophyll-a using appropriate software and algorithms. The algorithms are based on relationships between the chlorophyll-a concentration measured in-situ and the reflectance measured in the satellite images. The algorithm that would be tested relies on the ratio of suitable bands in the electromagnetic spectrum. The Oregon Fish and Wildlife services have set preliminary actions by taking chlorophyll measurements earlier this year that will be used to help draw the relationship between the measured and satellite derived chlorophyll-a concentrations. There exists a research group in the Civil and Environmental engineering department that have employed this process on the Deer Creek lake in Utah and other surrounding water bodies which has shown successful outcomes in monitoring these lake’s water quality parameters including chlorophyll-a to help support the survival of fishes, restore their habitats and preserve cultural history. There will be a comparison done to the performance of remote sensing models in a large, shallow lake in Oregon, compared to models developed in deep, narrow lakes.

Stephanie Mitts, Weber State University Engineering The recent development of rainwater harvesting (RWH) as a local government and individual property owner solution to stormwater management and water supply has led to a wide array of individual program implementations across the country. RWH involves collecting stormwater runoff, storing it and applying it for beneficial reuse or release at a controlled rate. Decreased need of freshwater withdrawals reduces hydrology based energy consumption and protects ecosystems, potentially making RWH a more sustainable and efficient practice than centralized water supply. The goal of this research project was to compile and analyze the national trends for local government urban rainwater harvesting program policy. A survey was created and administered to RWH managers across the country to collect U.S. policy information. This report contains information to be used as a guide for local governments and other institutions considering implementing a program to promote RWH.

Shana Black, University of Utah Engineering Goals: The pudendal nerve (PN) was targeted in attempt to create controlled micturition via intrafascicular electrical stimulation (IES) following the onset of surgically induced incontinence. We investigated both the effectiveness of unilateral and bilateral transection of the PN in creating a model of urinary incontinence and the ability of IES of efferent fibers to excite the external urethral sphincter (EUS) in order to restore a controlled voiding pattern. High Density Utah Electrode Arrays (HD-USEAs) were used to provide IES in these studies.

Jake Seiter, University of Utah Engineering Mining has always been among the most hazardous of occupations, and with the increasing demand for coal and minerals, safety in mines assumes even greater importance. Worldwide, underground miners are being exposed to noise and respirable dust hazards associated with roof bolt drilling. These hazards are now being understood to cause irreparable damage to the health of miners.

Charity Anne Rowley, Brigham Young University Health Background: Since 2009 groups of professors and students from the College of Nursing have been traveling to Guayaquil, Ecuador and assessing anemia rates and nutrition among the school children there. The adverse effects of anemia can include: weakness, fatigue, shortness of breath, and poor concentration. Initial anemia rates were discovered ranging 30-50%.

Brittni Carr, Brigham Young University Health Purpose/Aims: To examine religiosity and family hardiness in parents raising children with disabilities (CWD) to determine if there are differences according to parent gender and type of disability and if there is a relationship between the variables.

Matthew Mogensen, Southern Utah University Health The anti-inflammatory effects of omega-3 fatty acids (n-3) and their possible role in the regulation of asthma are promising for those suffering from the disease. Omega-3 fatty acids have been linked to the reduction of eicosanoids, which are signaling molecules that regulate the inflammatory response. Inflammation in the respiratory system is one of the most widespread symptoms of asthma and therefore poses the most risk to asthmatic individuals. This two phase study will test the effectiveness of n-3 in individuals with varying severities of asthma. In both stages of this study, a placebo or n-3 in the form of a krill oil capsule containing the omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) will be administered, lung volumes and capacities will be measured weekly, and the frequency/severity of asthma attacks will be recorded. Phase one of the study will test whether n-3 reduces asthma symptoms with no activity modifications, while phase two will test whether n-3 is effective in reducing asthma symptoms associated with exercise. The two phases of the study will be separated in order to isolate the duration of n-3 ingestion as a variable in the experiment. As this is an ongoing experiment, results are not yet available. However, if n-3 is effective, we expect to see an increase in lung volume and a decrease in the frequency and severity of asthma attacks in individuals. These results would suggest that n-3 could be an effective and simple treatment that, when taken daily, could reduce the frequency/severity of asthma attacks and help improve quality of life for asthma sufferers.

Andrew Mackay Breivik, Utah Valley University Health The central research question of this project is to determine the reproducibility of high frequency (HF) ultrasonic signals in breast cancer detection. Previous studies on surgical specimens of breast tissue have shown that HF ultrasound (20-80 MHz) appears sensitive to a range of breast pathologies including fibroadenomas, atypical ductal hyperplasia, fibrocystic changes, and carcinomas. A measurement in the ultrasonic signal called the peak density appears most sensitive to the pathology of the breast. The reproducibility of this parameter has not been quantitatively measured in a comprehensive manner. In parallel to a clinical study being conducted at the Huntsman Cancer Institute, we are conducting a laboratory study of the reproducibility of these measurements using chicken and bovine tissue. The ability to reliably determine the pathology of breast tissue with a real-time intra-operative tool would greatly aid in the surgical removal of all malignant tissue, as well as greatly reduce the occurrences of repeat surgeries to remove margins of cancerous tissue that remained. The results of this study will reveal the degree of variability in the signals, thus supplementing previous studies as well as determining the reliability of the results from the current clinical study. The research methodology included the following. Fresh chicken breast and bovine tissue were cut into 4x3x0.5 cm and 4x3x1.5 cm cubes. The tissue was tested at room temperature (23.4oC) using HF ultrasound. Pitch-catch and pulse-echo waveforms were obtained in triplicate measurements of two types: Three measurements with the transducer not leaving the tissue, and three measurements with the transducer lifted off the tissue between measurements. A total of 640 measurements were acquired and analyzed to obtain the spectral peak densities. Preliminary results indicate that the measurements are reproducible to a statistically significant level, thus removing one possible source of uncertainty in the data.

Kristen Hamblin, Brigham Young University Health Empathy is an essential characteristic in nursing. In fact, the skill of empathy, the ability to understand the feelings or situation of another, is of paramount importance to the nurse-patient relationship. Additionally, empathetic interactions often lead to increased patient satisfaction, compliance, and overall quality of life.

Siena Davis, Brigham Young University Health In the U.S., exposure to radon gas (222Rn) is the second leading cause of lung cancer after tobacco smoke. 222Rn is a naturally occurring environmental carcinogen produced during the radioactive decay of uranium-238, which is found in many soils throughout the U.S. Because 222Rn is a gas, it has the ability to travel through the soil and into homes through cracks in foundation walls. Inhaled 222Rn has the potential to emit alpha radiation in the lungs, and long-term exposure is responsible for approximately 21,000 lung cancer deaths per year in the U.S. 222Rn is a colorless, odorless, and tasteless gas, so the only way to know it is in a home is to test for it. Studies show that even in areas with high radon levels, homeowners frequently do not test or mitigate their homes as recommended. This study aims to understand the psychosocial factors that influence whether Utah residents perform radon testing and mitigation. Radon-related lung cancer deaths would be significantly reduced if the psychosocial factors that influence radon testing and mitigation were better understood and used to design and implement effective interventions. To understand the psychosocial factors that influence radon testing, we will survey residents (n = 50) of the county who come to UCHD specifically to purchase a radon test kit. The survey will be based on social cognitive theory (SCT) constructs. We will administer the same survey to a comparison group (n = 50). The comparison group for the study will be individuals who visit UCHD’s office of vital records. Individuals from the study group with indoor radon levels above 4 pCi/L will be contacted by telephone 30 – 60 days after testing to complete a second survey. This survey will measure SCT factors that influence participants’ actions to reduce radon levels in their home.

Teresa Wilson, Utah Valley University Health A critical issue in breast conservation surgery (lumpectomy) for breast cancer treatment is ensuring the tissue surrounding the excised tumor, the margins, are cancer-free. In collaboration with the Huntsman Cancer Institute at the University of Utah, students from Utah Valley University are using high-frequency (HF) ultrasound to test the pathology of lumpectomy surgical margins. This pre-clinical study is a blind study which will involve 80 patients, approximately 320 specimens, and use traditional pathology as the “gold standard” for measuring the HF ultrasound method’s accuracy. Ultrasonic waveforms of margins were acquired at the Huntsman Cancer Hospital in pitch-catch and pulse-echo modes using 50-MHz, 6.35-mm diameter transducers. The data were analyzed to obtain ultrasonic parameters such as wavespeed, attenuation, specimen thickness and spectral peak density (the number of peaks and valleys in a HF ultrasonic spectral band). The objective of this paper is to search for trends in the data acquired to date to provide an assessment of reliability, stability, and robustness of the study.

Ashley Ostler, Southern Utah University Health Research indicates that collegiate athletes may be at risk of developing disordered eating and other distorted behavioral patterns. This may be due, in part, to the aesthetic and performance demands of their sports. The purpose of this study is to discover if female athletes acquire increased negative views towards their body image and food due to the pressures of being a collegiate athlete at Southern Utah University (SUU). An anonymous survey was given to approximately 150 female athletes participating in a variety of sports inquiring about body perception and eating behaviors relative to their sport. The survey evaluates the athlete’s attitude toward food, their relationship with food, if there are outside influences that affect how they eat, and if they are satisfied with their weight. Athletic teams were chosen based on meeting two of the three following requirements: tryouts are held, scholarships are offered, and there are regular competitions or performances. Athletic teams involved in the survey include tennis, volleyball, track & field, cross country, softball, basketball, gymnastics, soccer, drill team, ballroom dance and cheer. The data will provide information regarding Southern Utah University female athlete’s body perceptions and eating behaviors. Results will be compared to a similar study conducted five years ago at SUU.

Taylor Colvin, Utah Valley University Health Introduction: There are millions of people in need of prosthetics worldwide and only a very small percentage currently have access to appropriate prosthetic care. As amputees are unable to afford a prosthetic, they are often incapable of maintaining many social interactions and provide for their basic needs. Utah Valley University’s Mobility Mission traveled to Guatemala to manufacture prosthetic limbs for those individuals in need. Our research can also encourage manufacturers to continue developing a better affordable prosthetic that can be donated to those amputees in need to restore quality of life to those individuals.

Josh Lee, Dixie State University Health According to the World Health Organization (2012), health care systems in Central America rank in the top 50% of the 190 ranked countries in the world, yet thousands of people are in need of simple medical attention and supplies every day in these areas. Countries such as Honduras lack virtually all access to health services, with 83% of the country’s population without health insurance, and 30% with no health care access at all (Pearson et al., 2012). To address this issue, a 2013 Dixie State University study conducted by the author, an Integrated Studies major with disciplinary emphases in Spanish and Biology, interviewed fifty adult Central American immigrants at a Southern Utah health care facility with the aim to providing student groups embarking on health service projects a researched list of vitally needed medical supplies. Participants in the study were asked to provide information regarding their health care experiences in their countries of origin and draw upon their comparative experiences with health care in the United States in order to determine areas of need. The forty individuals in this study represented a total of seven Central American countries and the interviews were conducted in Spanish and translated into English. The interviews were conducted over an 8-week period of time and results suggested that diabetes, cold, flu, antibiotics, and anti diarrhea medicines and supplies are a few of the needed health care items in these neighboring countries. Although statistical significance was not a feature of this study due to the number of participants, significant consistencies in responses by former residents of a variety of Central American countries suggested that some medical needs are regionally shared and that larger scale follow-up studies may reliably identify these as key resources for student medical delegations traveling to Central America.

Stephen Clarkson, Brigham Young University Health While there is an abundance of mobile health apps for weight management on the market today, almost all focus entirely on net caloric intake (exercise minus calories consumed). Recording daily caloric intake can be cumbersome, inefficient and inaccurate. One rising suggestion in the health field for reaching weight goals is objectively recording hand-to-mouth movements (HTMMs) during meals throughout the day. This method focuses entirely on portion control and if any improvements are to be made in this area, an effective method of activity recognition must be developed. In this paper, we report our efforts to classify HTMMs and non-HTMMs in an effort to automate counting the number of HTMMs during meals throughout the day. We also report on the performance of several base-level classifiers such as k-NN, Naive Bayes and Decision Trees as well as meta-level classifiers (Voting, Bagging and Boosting).

McKay Frandsen, Utah Valley University Health INTRODUCTION

Charity Anne Rowley, Brigham Young University Health In 2010, The Office of the Inspector General of the U.S. Department of Health and Human Services reported that adverse events during hospitalization contribute to the deaths of 180,000 patients each year! The purpose of this study is to improve patient safety in the hospital by understanding the role of the charge nurse(the 24/7 nursing supervisor in hospital units).

Jennifer Orton, Brigham Young University Health In contrast to the success of America’s vaccination rates among children, adult vaccination rates remain suboptimal. Some adults, such as school employees, are in a position where they can easily contract and spread vaccine-preventable communicable diseases, yet mandatory vaccinations are usually lacking in this population. Schools may easily become communicable disease outbreak centers, especially for highly contagious diseases such as measles and influenza. The purpose of this study was to evaluate why rural Utah school employees remain vaccinated for measles and influenza, as well as to discover the perceptions of these school employees regarding mandatory vaccination policies. Of the 835 respondents, 71.7% reported they received the measles vaccination as a child, although only 26.9% reported having a measles booster vaccine as an adult. When asked why they had not received the measles booster, 46% of participants stated they were unsure if they needed one. Other participants also reported lack of an adult measles booster vaccine because it was not required by the school district (5%) and that they did not perceive the need because they were healthy (5%). Even more concerning, over half of the school employees (51%) had not received their influenza vaccine for the current year. When questioned about their lack of influenza vaccination, 26.4% believed the vaccine was ineffective and 25.4% were noncompliant because they did not have the time to get the vaccination. When questioned about their perceptions regarding mandatory vaccine policies for school employees, four major themes emerged: 1) adults should retain agency; 2) vaccine effectiveness was not guaranteed; 3) adults do not need vaccinations; and 4) cost of vaccines. Further education is needed regarding the need for adult vaccinations, especially among school employees in rural Utah. To control the spread of communicable diseases, school districts may consider mandatory vaccination of school employees.

Emily Matson, Brigham Young University Health Cancer, despite its common association with old age, is a leading cause of death for children age 1-14, second only to unintentional injuries. Improvements in diagnosis and treatment are increasing childhood cancer survival, and more research is needed on the risk of subsequent cancer following an initial diagnosis, of either the same or another site. Research on associations between childhood and secondary cancers may improve patient follow-up by providing health professionals with information about which subsequent cancers are likely to occur, which screenings to conduct and what symptoms to look for.

Wesley Tietjen, Dixie State University Health High risk sexual behaviors, such as having unprotected sex, are a community health concern because of the heightened susceptibility to sexually transmitted diseases. An estimated 19.7 million people are newly infected with a sexually transmitted disease annually. Young people (ages 15-24) account for nearly half of all new incidences (CDC, 2008). Considering these statistics, the author, a Dixie State University Integrated Studies undergraduate student, conducted an interdisciplinary study to determine the correlation between the degree of self-reported religious devotion of Latter Day Saint (LDS) singles and their likelihood of purchasing or using protection during premarital intercourse. Seventy five students attending Dixie State University in Saint George, Utah participated in this study. Participants completed a 15-item survey consisting of questions inquiring how religious they consider themselves and their feelings towards premarital sex. Of the non-married participants who engage in sex, only 28% (n=4) of LDS respondents reported consistent use of birth control, compared to 84% (n=16) of non-LDS individuals. These results are suggestively significant and are consistent with findings from studies conducted by Douglas Kirby (2007) and others who have demonstrated that teaching abstinence, without providing education about birth control methods other than abstinence, leaves individuals unprepared to practice safe sex if the occasion arises. The relevance of the Dixie State University study to previous more broadly conceived research will be presented along with recommendations for future research.

Andrea Quiroz, Utah Valley University Health The stimulation and inhibition of tissue vascularization has important applications to tissue engineering and oncology. Approaches to quantitatively evaluate neovascularization in vivo in adult animals with differentiated tissue include both invasive methods that use an implanted or injury-induced matrix in the study organism, or noninvasive small animal imaging methods such as MRI, CT, and PET. The objective of this study was to determine if ultrasonic spectra in the 10-100 MHz range could be used as an in vivo neovascularization assay. Numerical simulations and phantoms were used as model systems to test the feasibility of the approach. The simulations modeled ultrasonic scattering from microscopic vascular networks using randomly oriented cylinders to represent blood vessels and cylindrical wave functions to represent ultrasonic waves. Phantoms were fabricated from a gelatin-soluble fiber mixture and agarose gel. The agarose gel was embedded in the gelatin-fiber in order to simulate vascular tissue. Ultrasonic tests were performed using two broadband ultrasonic transducers centered at 50 MHz. Pitch-catch data were collected and analyzed by calculating the peak density of the ultrasonic frequency spectrum, which is the number of peaks and valleys in the spectrum (a measure of the spectrum’s complexity). Control specimens were tested to determine the differences in the sound wave properties between the gelatin-fiber and the agarose gel. The controls demonstrated the instrument’s ability to differentiate between the two types of material. Measurements of agarose inclusions in the gelatin-fiber showed a significantly higher peak density than those for the control gelatin-fiber. These results indicate that the agarose gel structures are scattering sound to a greater extent than the gelatin-fiber alone. The results thus far have provided a strategy for proceeding with the project in the future. The phantoms will be refined and made more realistic to produce an adequate mimic for vascular tissue.

Elise Corbett, Brigham Young University Health Background

Tim Smith, Brigham Young University Health Previous research studies have confirmed that life-threatening illness adversely impacts psychosocial well-being, from feelings of isolation and embarrassment to the onset of psychotic episodes and suicidality. To help patients cope with chronic and/or terminal symptoms, many healthcare providers advocate the benefits of social support groups as a palliative measure, such as weekly meetings of cancer patients. The rapid proliferation of such programs, as well as associated “survivor groups,” has attracted research attention, and the number of research studies on the topic is now substantial. The field would currently benefit from a quantitative synthesis of the extant findings. To systematically quantify the effect of such intervention groups on mortality (i.e., death rates), we undertook a meta-analysis of the research literature, comparing patient survival rates as a function of social interventions. Across 27 studies examined thus far, we encountered a surprising variability of intervention success in prolonging life. Although most intervention provided demonstrated evidence of increased survival rates among participants, several studies did not. Our presentation will discuss the implications of our findings for healthcare providers and pose questions that will need to be addressed in future research.

Rhett Thomson, Utah Valley University Health Previous studies have shown high-frequency (HF) ultrasound may be sufficiently sensitive to detect and differentiate between both the histopathology and molecular subtypes of breast cancer. The goal of this study was to test the boundaries of HF ultrasonic testing methods. The parameter of interest was the effect of formalin fixation on tissue samples. Formalin has been found to stiffen tissues. Since ultrasound is sensitive to histological properties such as stiffness and adhesion factors, stiffening caused by fixation may compromise the results of the HF ultrasound analysis. This study monitored potential changes to HF measurements caused by tissue fixation. The fixation solution used was 10% neutral buffered formalin. The same formalin solution is used by hospitals after breast conserving surgery (BCS) to prepare and preserve excised tissue specimens for pathology. If no change in HF ultrasound analysis is detected in tissue samples before or after fixation, prepared tissue specimens from BCS patients may be used as viable samples for HF ultrasonic testing. Twenty tissue samples were used in this study. All samples were excised from the cortex, medulla, or ureter of a fresh cow kidney. Samples were tested with HF ultrasound. Testing conditions and procedures were identical to previous breast cancer tests. HF ultrasonic testing was completed at the zero hour mark and then repeated at five successive time intervals during fixation. Data collected from each sample was compared across all time intervals to detect any changes caused by fixation. Analysis of the data showed no significant changes in HF readings before, during, or after fixation up to 17 hours. Any variations observed were within experimental error. These findings demonstrate that formalin fixation does not affect the accuracy of HF ultrasonic testing techniques on bovine tissue samples. To reinforce the findings, this study will be repeated using different types of tissues.

Paydon Newman, Dixie State University Health Several studies (Shepley, 2012; Sherman-Bien, 2011; Walch, 2010) have found that sunlight has a stress-reducing effect on those who are exposed to it. An innovative new design of artificial lighting known as RaLight is proposed to reflect light with a color rendering index nearly identical to natural sunlight. This study will examine the relationship between exposure to RaLight (as a substitute for sunlight) and levels of stress. RaLight is predicted to decrease levels of stress in test subjects. In several hospice facilities, test subjects will consist of an estimated 100 patients both male and female with ages ranging from 65 to 85 years. This research is designed as a single subject experiment (ABA). The initial control condition will be the common indoor environment which exposes patients to standard fluorescent lighting. Replacing light fixtures with RaLight will then establish both the second phase of the experiment and the independent variable. A follow up re-installment of the initial fluorescent lighting will be conducted post-RaLight phase. Throughout each phase, questionnaires regarding the overall well-being of patients will be completed by hospice staff.

Nathan Bliss, Utah Valley University Health High-frequency (HF) ultrasound in the 20-80 MHz range has recently been found to be sensitive to pathology in tissue margins from breast cancer surgery. In order to improve the resolution and sensitivity of this method, however, transducers need to be employed that have piezoelectric elements that are smaller than those currently in use. The purpose of this study was to determine if similar results can be obtained from small element transducers (Blatek pachyometer, 50 MHz, element diameter < 2 mm) as compared to large element immersion transducers (Olympus NDT, V358-SU, 50 MHz, 6.35-mm diameter active element). Ultrasonic tests were performed on 10 bovine heart specimens of varying surface structure (myocardium, endocardium, and epicardium). Pulse-echo and through transmission measurements using a HF square-wave pulser/receiver (UTEX, UT340) and a digital storage oscilloscope (Agilent, DSOX3104A, 1 GHz, 4 analog channels) were acquired from a total of 2 sites per bovine specimen, first testing all specimens with the large transducers then again with the small transducers. Specimens were marked with India ink for location and accuracy of testing. The density of peaks in the ultrasonic spectra of the large transducers paralleled those of small transducers. Results from HF ultrasonic measurements of bovine heart tissue obtained from large transducers compared to the small transducers indicate that they produce statistically comparable peak densities.