2014 Abstracts

Whitney Badal, Brigham Young University Life Sciences Light therapy has been utilized to treat alcoholism and opiate-dependent rats as well as ameliorating symptoms of Parkinson’s disease. As both addiction and Parkinson’s Disease (PD) are often associated with decreased dopamine transmission in the striatum, it is likely that light therapy is able to increase dopamine release. A similar technique called near-infrared light treatment has also been shown to be effective in mice in restoring the function of dopamine cells in the substantia nigra pars compacta (an area associated with PD). A possible explanation for this is that light catalyzes the formation of neuromelanin. It is likely that neuromelanin is a neuroprotective cellular agent that is able to reduce damage caused by reactive oxygen species. Using UV-IR spectrophotometry, we show that in the presence of photostimulation, dopamine (0.3-30 uM) oxidizes and polymerizes into neuromelanin. Since hydrogen peroxide catalyzes this formation of neuromelanin, it is likely that this is a radical-polymerization reaction, suggesting that neuromelanin may be a radical scavenger. Additionally, the presence of the selective iron chelator desferrioxamine, the calcium chelator EGTA, or lack of calcium in the artificial cerebral spinal fluid markedly reduces the formation of neuromelanin. Using fast scan cyclic voltammetry in mouse horizontal and/or coronal brain slices, dopamine release in the nucleus accumbens core was enhanced by light exposure, in particular UV and short-wavelength visible light. These findings indicate that both iron and calcium are necessary for melanization in neural tissues and that light-induced melanization enhances dopamine release, suggesting a physiological role for melanization in synaptic transmission.

Chase Barker, Utah Valley University Life Sciences Central Research Question: Phylogenetic relationships of mayflies are still not very well known, however molecular and morphological data have begun to shed light on the relationships of these insects (Ogden et al. 2009). Our central question is to elucidate the phylogenetic relationships within the mayfly family Baetidae.

Ashley Calder, Utah Valley University Life Sciences An estimated 50-80% of dementia patients suffer from Alzheimer’s disease (AD). Currently there is no test to diagnose AD except post mortem. Recent papers indicate that AD affects the cytoskeleton and cellular structure through mutations that alter structural proteins, and that dysfunction of the cytoskeleton may play a pivotal role in AD and other neurodegenerative diseases. In particular, specific genetic components of AD affect microtubule and actin filaments that control endocytosis, exocytosis, the shape and size of the neuron, vesicular transport along neurites (dendrites and axons), and fibril formation. The goal of our research is to determine if breast cancer molecular subtypes can be used as a model for AD. Breast cancer is comprised of five molecular subtypes that contain different molecular structures depending on mutations specific to each subtype and the proteins being synthesized. These mutations and their expressed proteins change the characteristics of the cytoskeleton and resulting properties of the cell such as size, shape and stiffness. Both computer simulation and experiment have demonstrated that high-frequency ultrasound in the 10-100 MHz range is sensitive to these properties. For this study, ultrasonic tests were conducted on monolayer cell cultures of breast cancer cell lines of different subtypes. Ultrasonic waveforms were analyzed by transforming them into their corresponding spectra. The positions, widths, and shapes of the spectral peaks were compared and correlated to model results using a pattern recognition algorithm. Preliminary results indicate that cell stiffness and size can be determined from the measurements. Further analyses of these and additional data will determine if ultrasound is sufficiently sensitive to differentiate between the molecular subtypes of breast cancer. Results from these analyses, future studies with neuron cell cultures, and application of the results to the development of a minimally invasive, in vivo method for accurately diagnosing AD will be discussed.

Julia Hull, Weber State University Life Sciences Endophytes are fungi that live within aerial portions of plants for most or all of their life cycle without causing visible signs of disease. Gall forming aphids, Pemphigus betae, are highly competitive over gall site selection (Moran 1993), forming galls on the leaves of narrowleaf cottonwood and their hybrids. The favored gall location overlaps with areas of highest endophyte probability. I hypothesized that a negative correlation would exist between endophyte infection and aphid galling on leaves of backcross hybrid cottonwood trees.

Brianne Palmer, Utah State University Life Sciences Inversions have plagued the valleys in Utah resulting in built up pollution carpeting the cities and spreading into surrounding ecosystems. The ecological impact of these inversions is unknown. Inversion-based pollution events deposit nutrients and pollutants in the ecosystems. The purpose of this study is to determine if lichens, due to their unique physiology, preserve a record of inversion-based nitrogen deposition in order to assess the ecological impact of the inversions. In June and July of 2013 we collected 111 samples of lichens from sites exposed to the inversions, along Red Butte Creek and on an elevation gradient on Grandeur Peak in Salt Lake City. The collection focused on two species of lichens (Xanthomendoza montana and Xanthomendoza fallax) because they are easily identifiable and live in all the sampling sites. The samples were ground, run through a stable-isotope mass spectrometer and analyzed for the %N and δ15N. If lichens hold a record of inversion-based depositions there should be more nitrogen in lichens from the sampling sites closer to the pollution source (Salt Lake City). Once the data was complied, the resulting graphs showed no correlation between %N and distance from Salt Lake City between δ15N and the pollutant source. However, the isotopic variability within the samples was greater than expected and requires further research.

Preston Manwill, Southern Utah University Life Sciences Plants produce compounds, secondary metabolites, which can be harnessed for medicinal uses. Caryophyllene, a secondary metabolite produced by a variety of higher plants, has shown promise as an analgesic and anti-inflammatory. Caryophyllene synthase is the final enzyme in the biosynthetic pathway that produces caryophyllene. The gene and enzyme responsible for caryophyllene production have been observed in crop plants and model organisms, but few studies have investigated caryophyllene production in indigenous plants. We investigated the production of caryophyllene in Ericameria linearifolia (Narrowleaf goldenbush) because it is a shrub native to southwest North America. The first portion of this research examined sequence similarities between caryophyllene synthase gene orthologs in E. linearifolia and other plant genera. Genomic DNA was isolated from E. linearifolia leaves collected from plants growing in southern Utah. Literature reviews and database inquiries have provided DNA sequences for similar synthases, from which primers will be designed to copy and sequence the E. linearifolia caryophyllene synthase gene, confirming the orthologs presence. Following confirmation of the gene, mRNA will be extracted from leaf material and oligo(dT) primers used to synthesize cDNA. Subsequent cDNA amplification and cloning of products into a vector will allow for transformation into a protein-producing bacterium. These final steps make the downstream procedures of protein production, structure determination, and functional characterization of the protein possible. Comparison with enzymes that produce similar organic compounds may result as well, and would provide insight that could improve the biosynthesis of medicinal compounds.

Preston Manwill, Sourthern Utah University Life Sciences Plants produce myriad secondary metabolites (essential oil) that play a role in ecological interactions. Species evolve unique mixtures of organic compounds due to strong selective pressures that act on metabolic pathways. Few studies have investigated the antimicrobial properties of secondary metabolites that evolved against soil microbes associated with native plants. The essential oil of Mojave goldenbush (Ericameria linearifolia), a shrub native to the Intermountain West, was characterized and its bacteriostatic abilities against root associated and non-root associated bacteria morphs were examined in this study. We hypothesized E. linearifolia oil would act as a greater growth inhibitor against native soil bacteria with which it has evolved than against bacteria from non-associated soils. Secondary metabolites were extracted using steam distillation and analyzed with gas chromatography mass spectroscopy. A Kirby-Bauer assay tested presence, size and retention of inhibition zones (IZ) produced against soil bacteria. The essential oil of E. linearifolia contained twenty secondary metabolites, two of which, limonene and sabinene, constituted greater than fifty percent of the oil. Presence and retention of IZ produced by E. linearifolia metabolites varied significantly by community association and bacteria morph. The hypothesis was supported by IZ that were largest against bacteria cultures isolated from soil that surrounded Mojave goldenbush roots and least effective against cultures isolated from non-root associated soil. Our findings suggest that secondary metabolites of E. linearifolia have evolved to specifically prevent negative interactions with bacteria most associated with the plant.

Cameron Sargent, Brigham Young University Life Sciences Although viruses present a potential solution for treating and studying many harmful diseases, they have yet to be utilized successfully, largely due to an overall lack of understanding of the interactions between viruses and their hosts. This study, however, demonstrates the efficacious usage of comparative genomics in further elucidating viral reproduction mechanisms, a step in overcoming this hurdle. Following the sequencing and annotation of genomes of the B4 subcluster of mycobacteriophage, a previously unstudied selection of viruses, comparative genomics analysis identified regions of proteins from these viruses that are highly conserved, or highly similar. One of these B4 phage proteins was then compared on the basis of its predicted folded structure and its amino acid composition to a protein with the same function from another phage, whose structure and DNA binding mechanism was determined via NMR spectrometry. Gene sequence alignments identified the region of highest conservation in the B4 phage. Protein folding then revealed that this region created a structure that was analogous to the functional region of the protein studied elsewhere, indicating that it too recognizes, binds to, and modifies DNA in the same manner. Furthermore, another DNA-binding motif with high conservation among B4 phage was observed in both the folded B4 proteins and the protein analyzed via NMR spectrometry. This comparison procedure not only further elucidated the function of an important gene in B4 phage but also displayed that comparative genomics, a resource-effective and computer-based approach, can successfully identify the critical functional regions of a protein. This study therefore provides a novel procedure for further understanding the mechanisms of virus-host interactions, which in turn facilitates the successful application of viruses in treating and studying diseases.

Tamara Fox, Weber State University Life Sciences Clostridium botulinum has been implicated in cases of infant botulism across the United States. It is recommended that infants under the age of one year not be fed honey because of the presence of C.botulinum spores. The goal of this project is to determine whether honey produced in small and large apiaries in Utah contain varying amounts of toxin producing C. botulinum. Honey samples will be collected from hives maintained in Utah and tested for the presence of toxin producing strains of C. botulinum. Samples will be dissolved and centrifuged to isolate the spores and then superheated to release the DNA. Testing will then be done through a multiplex polymerase chain reactions (PCR) using primers specific for 16s rRNA, Clostridia species, and toxins A, B, E, and F. The presence and type of toxin producing Clostridia species will be compared with a Chi-Squared Test of Independence. Research will be completed by February of 2014 and we expect small apiaries will have a lower frequency of toxin producing C. botulinum strains than large apiaries and that toxin phenotype will vary between the two groups. The results will increase understanding on the variance of C. botulinum in Utah honey and will contribute to further research on this topic.

Kevin Nay, Southern Utah University Life Sciences Leech taxonomy has traditionally been based on morphological characters, but with new developments in DNA technology many taxonomists are starting to use genetic information in descriptions of new species. Leeches in southern Utah are poorly inventoried with respect to many other aquatic animals. There have been few morphological inventories of leeches and even fewer descriptions of the genetic diversity within leeches. Landscape genetics is a powerful tool used to understand geographic patterns of genetic diversity. Southern Utah has many naturally isolated bodies of water due to the climate and the dramatic changes in elevation in this part of the country. The landscape genetic study of leeches in southern Utah will provide us with a better understanding of genetic differentiation within southern Utah leeches. The mitochondrial DNA (CO I region) will be used to estimate genetic diversity and examine the relationships among individuals in two populations of leeches. I hypothesize that leeches in southern Utah will have greater genetic diversity then historically recognized from morphological studies suggesting a new species of leech. The study will lead to better understanding of the taxonomy and identification of southern Utah leeches.

Ashley Nelson, Brigham Young University Life Sciences The motivational effects of opiates and ethanol switch from a dopamine (DA)-independent to a DA-dependent pathway when the animal is in a drug-dependent state. A corresponding change occurs in ventral tegmental area (VTA) GABA(A) receptors in opiate-dependent animals, which switch from a GABA-induced hyperpolarization of VTA GABA neurons to a GABA-induced depolarization. The aim of this study was to evaluate VTA GABA neuron excitability, GABA synaptic transmission to VTA GABA neurons and GABA-mediated DA release in the nucleus accumbens (NAc) under ethanol-naïve and dependent conditions. To accomplish these studies, we used standard whole-cell and attached-cell mode electrophysiological techniques to evaluate acute and chronic ethanol effects on VTA GABA neurons in GAD GFP mice, which enabled the visual identification of GABA neurons in slice preparation. In naïve animals, superfusion of ethanol (IC50 = 30 mM) and GABA(A) receptor agonist muscimol (IC50 = 100 nM) decreased VTA GABA neuron firing rate in a dose-dependent manner. Compared to saline-injected controls, in animals made dependent on ethanol by twice daily injections of 2.0 g/kg ethanol, neither ethanol nor muscimol significantly affected VTA GABA neuron firing rate on average. We and others have found that ethanol decreases DA release at terminals, as measured by fast scan cyclic voltammetry. We have recently reported that ethanol inhibition of DA release at terminals in the NAc of ethanol-naïve animals is mediated by GABA, possibly from VTA GABA neurons that project to the NAc. We evaluated the effects of ethanol on DA release in the same ethanol-dependent animals. Compared to controls, superfusion of ethanol did not significantly affect DA release. Together, these findings suggest that VTA GABA neurons undergo a switch in GABA(A) receptor function with chronic ethanol, which results in a corresponding switch in DA release, perhaps resulting from adaptations in VTA GABA neuron input to the NAc.

Melanie Long, Westminster College Social and Behavioral Sciences While differences in hourly earnings between men and women have frequently been investigated in gender economics, the gender gap in unemployment rates has received less attention. According to much of the existing research, the reduction in the unemployment rate differential between the genders over the past 30 years indicates that gender no longer plays a significant role in employment outcomes. This regression analysis evaluated that claim using microeconomic data from the Bureau of Labor Statistic’s National Longitudinal Survey of Youth. Panel data regressions were conducted to establish whether women are subject to more weeks of unemployment in a year than men and whether this difference represents the influence of direct and indirect (i.e., societal) gender discrimination. After accounting for women’s decisions to drop out of the labor force, the regression output indicates that direct gender discrimination against women may no longer play a significant role in employment outcomes. However, there is evidence that societal pressures continue to encourage women to quit work in response to unemployment more readily than men. These findings speak to the complex gender dynamics that persist in the American labor market despite measures to ensure equal employment opportunities and suggest that unemployment rates alone are insufficient to fully understand those dynamics.

Erin Martin, Weber State University Social and Behavioral Sciences There is a rise in the use of drugs and alcohol in college students across the nation. College students are becoming dependent on drugs long after the books have closed and college students have tossed the caps, turned in their gowns, and received their diplomas. The purpose of the study is to determine at what rate is the use of drugs and alcohol being experimented within college dorm rooms. Furthermore, this study will discover whether a person’s gender plays a role a determining if college students are more likely to experiment with drugs or alcohol, because they live in campus dorms. The method of this study is a survey at Weber State University. I will be looking at two different populations, those who live on campus at Weber State University and those who live off campus. I will survey students living in University Village, those in classrooms and those around campus. When the responses come back, the responses are going to be divided by those who live in the college dorms and those who live off campus. They will be further separated by male and female, in order to determine which group is more likely to experiment with alcohol and drugs. My hypotheses is that Weber State University has a smaller housing option for students than most other universities and being a commuter school, the results will be different from those found in other studies. There might not be a large problem of illegal drug abuse at Weber State University; however, you will see abuse in alcohol and prescription drugs.

Benjamin Hardy, Brigham Young University Social and Behavioral Sciences Informed by I3 theory, three studies examine whether engagement in extradyadic behavior is related to aggressive behaviors. It is hypothesized that extradyadic behavior is related to heightened aggression toward a romantic partner. Study 1 (n=97) tested the relationship between extradyadic behavior and anger and found that acts of extradyadic behavior were related to increased use of anger words when describing a recent transgression of a relationship partner. Study 2 (n=72) tested the relationship between extradyadic behavior and aggressive behavior during an interaction. It was found in Study 2 that reports of extradyadic behavior were linked to hostility, criticism, and frustration expressed to a relationship partner. Study 3 (n=99) longitudinally examined the relationship between extradyadic behavior and aggression, and found that extradyadic behavior at Time 1 predicted aggression toward a relationship partner at Time 2, controlling for initial aggressive behavior. Study 4 (n=473) again longitudinally examined the relationship between extradyadic behavior and aggression using a larger sample and longer time frame of twelve weeks and found that extradyadic behavior at Time 1 predicted aggression toward a relationship partner at Time 2, controlling for initial aggressive behavior.

Garrett Nagaishi, Brigham Young University Social and Behavioral Sciences Ottobah Cugoano, one of the many black voices from the eighteenth-century Atlantic world, professed that he and millions of other slaves “look for our help and sure deliverance to come from God Most High” (Ottobah Cugoano, Thoughts and Sentiments, 144) Cugoano’s zeal was not unique – many black writers in Europe and the Americas likewise found peace in religious devotion that transcended geographical and cultural boundaries. Yet despite the promises of salvation and peace that religion offered to its followers, slaves were constantly forced to reconcile an uncomfortable reality: that the religion which they literally clung to for dear life was the same faith that their unmerciful masters professed. How were slaves able to come to terms with the blatant shortcomings of religious rhetoric? And for the slave who was not born into Christianity, what kind of connection did he form with his new religion that enabled him to see past the ironies? My paper suggests that the very nature of Christianity presupposed the fallibility of human nature and encouraged the most abject person to rise above adversity with hope in Jesus Christ. For those slaves who were not born into the religion, their close contact and interaction with Christian slaves created a support system among slaves which was based on Christian theology. Furthermore, many slaves crossed the infamous Middle Passage during their infancy and youth, an impressionable period of life, which would have enabled Christianity to more readily appeal to the slave’s psyche. Thus, for many slaves in the eighteenth century, sinful masters and hypocritical evangelists were not seen as a stumbling block to faith; rather, they confirmed the necessity of Christian faith as God’s prescribed method for overcoming an otherwise miserable existence.

Michael Gillespie, Brigham Young University Life Sciences It is vital to understand the anatomical microstructure of tendons and ligaments in order to ascertain their specific qualities and functions. Recent developments in micro-scribe 3D digitization are highly effective in revealing these intricacies. A necessary component to creating 3D fiber maps from this technology is the ability to distinguish between individual tissue fibers with the naked eye. However, this is a very difficult task with most tendons and ligaments. We developed a paste made of blue dye and powdered sugar that when applied, fits in-between these fibers and contrasts the specimen color. This exposes the fine architecture, making individual fibers much more visible and thus able to be 3D digitized. With these 3D fiber maps now available, tendon and ligament microstructure can be viewed in greater detail than previously possible. This technique was applied to human cadaveric calcaneal tendon and quadriceps tendon. It was discovered that the fibers of human calcaneal tendon have higher overlap relative to the rigidly parallel fibers of the quadriceps tendon. This further understanding carries implications regarding the advancement of biomechanical models, artificial reconstruction, and surgical repair of these tissues. It also highlights the need for further investigation into the microstructure differences among tendons and ligaments.

Michaelle Cadet, Utah Valley University Life Sciences Utah County, Utah has an estimated population of 540,000 residents and is considered to be a non-attainment area for criteria pollutants such as PM-10 and CO. High levels of these contaminants may increase the risk of respiratory diseases. Additional environmental issues exist including water contamination and eutrophication of Utah Lake. These environmental issues are frequently on the news and warnings are issued by the Department of Environmental Quality notifying citizens of the potential health concerns associated with environmental pollution. With these announcements, it is expected that Utahans are acutely aware of environmental issues, particularly, in the academic settings. The purpose of this study was to investigate the status of environmental awareness among students at Utah Valley University and to correlate the results to socio-demographic categories. Utah Valley University, located in Utah County, is a public institution of higher education with approximately 30,000 registered students. Data for this study was collected through a survey using a multistage sampling technique with population stratified based on colleges and schools within the university. Sample size included approximately 1,000 students. The survey contained 12 environmental questions relating to recent local, national and international media exposure. Standards and protocol of the International Research Board were employed. We hypothesize that married, educated, religious males will positively correlate with higher levels of environmental awareness, but will be less than atheists. Additionally, those identifying with the Republican Party will have lower levels of awareness. No significant difference will be found between majors. Furthermore the young, poor and ethnic populations will be less aware than their more affluent counterparts. Results of this study will be used to inform residents of environmental issues and the associated health concerns. Additionally, this study will be used to inform legislators about the importance of environmental education in the community.

J David Symons, University of Utah Life Sciences Cardiovascular complications (e.g., arterial dysfunction) are more prevalent in patients with type 2 diabetes (T2DM). Patients with T2DM have elevated levels of circulating free fatty acids (FFAs). We have shown that when bovine aortic endothelial cells (BAECs) are treated with the physiologically relevant FFA palmitate, protein phosphatase 2A (PP2A) activity increases, phosphorylated endothelial nitric oxide (NO) synthase (eNOS) to total eNOS (p-eNOS:eNOS) decreases, and metabolites of NO production decrease. NO is an important endothelial-derived relaxing factor that is vasculoprotective. As such, FFA-induced, PP2A-mediated reductions in p-eNOS:eNOS and NO production might explain why vascular complications are more common in pathologies associated with lipotoxicity e.g., T2DM and diet-induced obesity. Recently we showed in BAECs that if PP2A is inhibited using okadaic acid (OA), palmitate-induced increases in PP2A activity, and reductions in p-eNOS:eNOS and indices of NO production are negated. We sought to translate these finding from BAECs to the intact organism. However, OA cannot be used in vivo. Lixte Biotechnology 1 (LB1) is a PP2A inhibitor that has been used in vivo in the context of cancer research. The purpose of this study was to determine the efficacy of LB1 under our experimental conditions, with the long-range goal of using LB1 in mice. Further, we used this opportunity to optimize the measurement of NO directly using electron paramagnetic resonance spectroscopy (EPR). BAECs were treated for 3 h with vehicle (V), 500 µM palmitate (P), 4 µM LB1, or P + LB1 (n=10 per treatment). P increased (p<0.05) PP2A activity (50±12%), and decreased (p<0.05) p-tyr307:PP2A (29±9%; redundant indicator of increased PP2A activity), p-eNOS:eNOS (30±3%), and NO production (27±9%). All P-induced effects were prevented by concurrent treatment with LB1. Future experiments will determine whether chronic treatment of mice with LB1 is capable of suppressing PP2A activity in intact arteries.

Audrey Butler, Utah Valley University Life Sciences High-frequency (HF) ultrasound has been shown to be sensitive to a range of breast pathologies, and is being explored for the intra-operative assessment of lumpectomy margins. This sensitivity is believed to arise from microstructure-dependent interactions of ultrasound in the tissue. The objectives of this study were to develop breast tissue phantoms with microstructures that accurately mimic the histology of normal and malignant tissue, and to determine the effects of these microstructures on HF ultrasonic spectra (10-100 MHz). Phantoms were created from a mixture of water, gelatin, and soluble fiber. To simulate various breast tissue histologies, polyethylene beads, polyethylene fibers, and nylon fibers with a range of diameters were embedded into phantoms. Microstructures ranging from randomly dispersed beads to bead-fiber constructs resembling terminal ductal lobular units (TDLUs) were modeled and tested. Pitch-catch and pulse-echo measurements were acquired using 50-MHz transducers, a HF pulser-receiver, and a 1-GHz digital oscilloscope. Spectra were derived from the data and peak densities were determined from the spectra. Peak density, which is the number of peaks and valleys in a specified spectral range, has been shown to correlate with tissue complexity. Preliminary results from dispersed beads (58-925 µm diameter) of constant volume concentration (0.8%) indicated that the smaller beads produced higher peak densities than the larger beads with a consistent and statistically significant trend. These results substantially improve upon previous phantom studies and upon results from original breast cancer studies, demonstrating the strength of the HF ultrasound response to tissue microstructure. The higher peak densities can be attributed to either the higher number of scatterers for small beads or the size of scatterer in relation to the ultrasonic wavelength. These and other results from more advanced histologically accurate microstructures modeling TDLUs will be discussed.

Jordan Fenlon, University of Utah Life Sciences Tsr, the serine chemoreceptor for E. coli, is a transmembrane protein with a periplasmic sensing domain and cytoplasmic adaptation and kinase control domains. The focus of my research project is Tsr residue A413, located in the cytoplasmic tip of the receptor’s kinase control domain. The project involves characterization of mutant Tsr proteins containing amino acid replacements at residue 413. Based on previous work in the Parkinson lab, this residue is thought to play a key role in Tsr signaling by regulating the dynamic motion of the tip.

Josh Harvey, Brigham Young University Life Sciences Soil CO2 flux represents an important pathway of carbon transfer from ecosystems to the atmosphere. Soil CO2 flux can be altered by global warming-driven changes in seasonal temperature and water availability. Subalpine ecosystems have high levels of carbon in their soils that are stabilized by low temperatures and low microbial activity during long and snowy winter seasons. Subalpine ecosystems can be important sinks for carbon, storing carbon that otherwise would be in the atmosphere contributing to global warming. In our study we show how changes in temperature and water availability during springtime increase the levels of subalpine carbon output. So long as the carbon outputs outweigh carbon inputs, increases in soil flux would amplify global warming. The amplification of global warming would loop back to affect soil fluxes again (by raising temperatures, melting snow earlier, and changing precipitation patterns) thus creating a positive feedback system. Understanding what feedbacks are present in a climate system and their underlying mechanisms will improve our forecasts of changes in atmosphere chemistry and temperature.

Evan Campbell, Brigham Young University Life Sciences Asthma is a chronic allergic disorder manifest by airway restriction due to inflammation, bronchoconstriction, and increased respiratory mucous secretion. As many as 300 million people worldwide are affected by asthma and its prevalence is increasing primarily in countries experiencing urbanization and Westernization. Asthma is currently the most common chronic illness among children in the U.S., and the third leading cause of hospitalization for children aged 0 – 15 yrs. Reservoir dust collection and area air sampling are the two primary methods of measuring allergen levels in house dust. Allergen sensitization leading to asthma is thought to occur prior to age six while the immune system is still naïve. In the case of the dust mite allergen Der p1, the exposure window may be as early as age two. However, little evidence is available to establish a dose–response relationship between inhalation exposure and early immunological sensitization to allergens. Temperature and relative humidity play a major role in dust mite survival and proliferation and indoor humidity above 50-60% in arid environments has been shown to support dust mite populations. Evaporative “swamp” coolers cool air by adding humidity to it and can create favorable environments for dust mite survival. We are working to determine how much swamp coolers contribute to dust mite levels in Utah by quantitating dust mite allergen levels in homes with and without swamp coolers. In order to do this we are developing sensitive ELISA and quantitative PCR methods to allow us to determine levels of exposure even when low levels of dust are collected.

Logan Warner, Utah Valley University Life Sciences High-frequency ultrasound (20-80 MHz) has been found to be sensitive to a range of pathologies in excised breast tissue before fixation in formalin or other formaldehyde analogues. Formalin fixation, however, may alter the structure and rigidity of a sample so that data gathered using high-frequency ultrasound after fixation may no longer be viable for research purposes. This limits the amount of time researchers may conduct tests, so preservation via simple sugars is being considered. Numerous studies have been conducted using sucrose, trehalose, or glucose as cryoprotectants for cells and simple tissues. The objective of this study was to test the sensitivity of high-frequency ultrasound to changes in the microstructure, stiffness, and cellular integrity of tissue samples due to cryopreservation with these sugars. Domestic pig heart tissue was placed in aqueous solutions of sucrose, trehalose, and D-(+)-glucose. The specimens were refrigerated and observed over time using high-frequency ultrasound to detect tissue damage. The results of this study suggest that cryopreservation with sugars will not only allow more time for researchers to conduct ultrasonic tests on surgical specimens, but also that high-frequency ultrasound could potentially be used as an assay to measure tissue degradation in preserved living tissues such as transplant organs.

Hillary Hansen, University of Utah Life Sciences Type 1 diabetes is an autoimmune disease where pancreatic β-cells are destroyed, resulting in insulin deficiency. Generating new β-cells from stem cells for treating diabetes will benefit from understanding their development in vivo. Pancreatic β-cells, along with all other pancreatic lineages arise from multipotent pancreatic progenitor cells (MPCs). Previous studies demonstrate that the structural and signaling protein β-catenin is required for the development of the exocrine acinar lineage. β-cells still differentiate in the absence of β-catenin, however, β-cell mass is dependent upon β-catenin. We determined that this dependency reflects a role for β-catenin in the maintenance of MPC patterning as well as for expansion of the progenitor pool. Whether our observed effects are due to the signaling or structural function of β-catenin remains unknown, and is the focus of this research. Using mouse genetics we are able to separate the structural and signaling functions of β-catenin. Eliminating both functions in PBKO (full knockout) mice produces decreased β-cell mass and irregular patterning. Decreased β-cell mass is also observed in PBsKO (signaling deficient) mice, though patterning remains unaffected. This suggests that pancreas growth is dependent upon canonical Wnt/β-catenin signaling, and that maintaining progenitor identity requires the structural role of β-catenin. Elucidating distinct roles for β-catenin could be used to drive stem cell-derived MPCs to expand and differentiate to the desired pancreatic cell fate.

Michael Naegle, Brigham Young University Life Sciences Dermaptera is a comparatively small order of insects with approximately 1800 species placed in three suborders. While the majority of earwig species are placed within the suborder Forficulina and are free-living with forceps-like appendages, two dermapteran lineages have a very unusual morphologies and life histories. The viviparous Hemimerina live epizoically on giant rats in tropical Africa where they feed on fungi growing on the rats’ skin. Hemimerina lack eyes and wings and the cerci are filiform. The viviparous Arixenina are associated with bats in Malaysia and the Phillippines, and they feed on bat skin gland secretions. They have reduced eyes, are wingless, and possess straight cerci. The phylogenetic position of the suborders Arixenina and Hemimerina relative to Forficulina have previously been unclear; however preliminary analysis suggest the phylogenetic position of the suborders Arixenina and Hemimerina are nested within Forficulina, with ectoparasitism evolving multiple times within this order. We generated DNA sequence data from three nuclear (18S, 28S and H3) and two mitochondrial (COI and TUBA) genes for representatives of all three suborders and outgroups. A phylogeny was reconstructed to address the following questions: (1) Does Hemimerina + Arixenina form a monophyletic group and support a single origin of parasitism or are there multiple origins of parasitism? (2) Is Forficulina monophyletic with respect to these parasitic lineages? (3) Are morphological similarities shared by the ectoparasitic forms synapomorphic or homoplasious characters?

John Hancock, Brigham Young University Life Sciences LLO56 and LLO118 are CD4+ T cells specific for the same Listeria monocytogenes epitope. Despite their TCRs differing by only 15 amino acids, LLO118 and LLO56 have dramatically different primary and secondary responses to Listeria monocytogenes infection. We reasoned that LLO56, the single chain TCR (Vβ2-linker-Vα2) could be subjected to directed evolution to generate mutants that are more stable and bind to peptide-MHC with higher affinity. Single chain LLO56 was fused to the yeast surface protein Aga-2 and error prone PCR was used to generate mutagenic libraries. A first generation stabilized single chain TCR (scTCR) was selected using biotinylated Vβ2 and Vα2 antibodies and anti-biotin beads. The first generation LLO56 mutant expressed LLO56 on the surface of yeast at higher levels than wild type by flow cytometry. To produce mutants with additional stability, a second-generation mutant was generated by combining multiple stability mutations isolated in a number of first generation clones.

Madison Peterson, Utah Valley University Life Sciences High-frequency (HF) ultrasound in the 20-80 MHz range has recently been found to be sensitive to pathology in tissue margins from breast cancer surgery. In order to improve the resolution and sensitivity of this method, however, transducers need to be employed that have piezoelectric elements that are smaller than those currently in use. The purpose of this study was to determine if small element transducers (Blatek pachyometer, 50 MHz, element diameter < 2 mm) produce similar results as those obtained from large element immersion transducers (Olympus NDT, V358-SU, 50 MHz, 6.35-mm diameter active element). Ultrasonic tests were performed on 10 phantom breast samples made of Knox gelatin base and soluble fiber (Metamucil), five of which contained chopped nylon fibers and five which contained polyethylene microspheres. Pulse-echo and through transmission measurements using a HF square-wave pulser/receiver (UTEX, UT340) and a digital storage oscilloscope (Agilent, DSOX3104A, 1 GHz, 4 analog channels) were acquired from a total of 3 sites per phantom in triplicate, first testing all specimens with the large transducers then again with the small transducers. Specimens were marked with India ink for location and accuracy of testing. The density of peaks in the ultrasonic spectra of the small transducers paralleled those of large transducers. Results from HF ultrasonic measurements of phantom breast tissue obtained from small transducers compared to the large transducers indicate that they produce statistically comparable peak densities. In breast conservations surgery it is crucial to excise all cancerous tissue to prevent recurrence. This method could provide in vivo cancer detections in margins and allow for more precise excision of tumors and cancerous tissue preventing the need for subsequent surgeries and thus, less risk, reduced pain and suffering, lower costs and better outcomes for breast cancer patients.

Brian Ballard, Brigham Young University Life Sciences Antigen presenting cells digest and display proteins from foreign and infected cells on the major histocompatibility complex (MHC) which can then be recognized by T-cells through their T cell receptor (TCR). LLO56 and LLO118 are CD4+ T cells specific for the same Listeria monocytogenes epitope but show dramatically different primary and secondary responses to infection. Because TCRs have very low affinity for MHC we would like to create a high affinity T cell. We reasoned that the single chain LLO118 TCR (Vβ2-linker-Vα2) could be subjected to directed evolution to generate mutants that are more stable and then used as a template for engineering high affinity T cell receptors. Single chain LLO118 was fused to the yeast surface protein Aga-2 and error prone PCR was used to generate mutagenic libraries. The first generation stabilized LLO118 single chain TCR (scTCR) was selected using biotinylated Vβ2 and Vα2 antibodies and anti-biotin beads and it expressed LLO118 at higher levels than wild type by flow cytometry. To produce mutants with additional stability, a second mutagenic library using the first generation mutants as templates has been produced and the most stable clones will be selected after temperature denaturation, permitting isolation of clones with increased stability for generating high affinity pathogen specific scTCRs. After engineering a high affinity T cell our research will further understanding on TCRs and the MHC and could also serve as a resource for creating a therapeutic drug.

Lance Deeter, University of Utah Life Sciences Continuous-flow left ventricular assist devices (LVADs) are used in advanced heart failure patients either to bridge them to transplantation or as a permanent-destination therapy. We determined whether chronic exposure to non-pulsatile blood flow and acute increases in coronary perfusion pressure associated with LVAD implantation would influence arterial function. Arteries from a transmural biopsy of the left-ventricle were obtained from ten male patients (54±4 years old) at the time of LVAD implant (n=17, 184±25 µm i.d.) and 239±51 days later upon LVAD explant (n=21, 281±22 µm i.d.). Lmax tension was determined and dose-response curves to potassium chloride (KCl, 10-100 mM) were performed using isometric tension techniques. Next, bradykinin (BK, 10-6 to 10-10 M) and sodium nitroprusside (SNP, 10-4 to 10-9 M) concentration-response curves were completed on vessels precontracted to ~65% of maximal tension development. Maximal BK-induced vasorelaxation was greater (p<0.05) at explant (85±5%) vs. implant (59±9%), while SNP evoked responses (~90%) were similar between time-points. These findings suggest coronary endothelial function is improved by LVAD implantation. Heart failure was precipitated by a myocardial infarction in six of the ten patients. These are referred to as “ischemic” patients whereas the remaining four are “non-ischemic” patients. We hypothesized that coronary vascular responses would be improved by LVAD implantation to a greater extent in ischemic vs. non-ischemic patients. In ischemic patients maximal BK-induced vasorelaxation was greater (p<0.05) in coronary arteries obtained at explant (87±6%, n=14, 305±30 μm i.d.) vs. implant (53±11%, n=12, 204±33 μm i.d.). In non-ischemic patients maximal BK-induced vasorelaxation was similar in arteries obtained at explant (79±9%, n=7, 232±21 μm) and implant (72±17%, n=5, 135±13 μm). SNP responses were similar (~90%) between groups at implant and explant. Collectively, our data suggest that LVAD implantation improves endothelium-dependent vasorelaxation in ischemic but not in non-ischemic patients.

Colby Pearson, Utah Valley University Life Sciences Many lichens synthesize unique secondary metabolites, such as atranorin (AT), which may serve as photo-protection against harmful UV radiation. Our study investigates changes in metabolism and internal levels of atranorin in lichens under various light conditions and atranorin supplementation. We will expose 60 Physcia adscendens (Fr.) H. Olivier lichen thalli to one of three different light levels (UV +PAR; PAR only; and neither UV nor PAR). Half of our experimental thalli will be supplemented with AT. Lichen biomass, AT concentration via HPLC, and photosynthetic and respiration rates will be measured before and after the experiment to measure metabolic responses of both supplemented and non-AT-supplemented thalli under each light level.

Robert Erickson, Utah Valley University Life Sciences Although the insect fauna of the Colorado Plateau region are somewhat well known, our specific understanding of the arthropod biodiversity in Capitol Reef National Park is sparse.

David Vogelsang, Brigham Young University Life Sciences More than one-third of U.S. adults (35.7%) are obese (CDC, 2013a), and since 1980, obesity among adolescents has risen from 5% to 18% (CDC, 2013b). Unless we do something to combat the growing obesity epidemic, we are consigning ourselves and future generations to a lifetime of heart disease, diabetes, cancer, and psychological distress (CDC, 2013a). Cooking Anatomy Academy (CAA) promotes healthy eating and cooking among parents and students in the Polynesian community to raise awareness about the growing obesity epidemic. Our primary focus is introducing parents and students to healthy, great tasting foods and easy to prepare meals (Brown, 2011). Our secondary focus is to teach the simple anatomy and physiology important to understanding healthy nutrition. CAA is composed of seven, one hour lessons that are being offered as an afterschool program at Mana Academy Charter School. We’ve developed the CAA curriculum to incorporate many of the nutrition guidelines on MyPlate.gov, and focus on moderation, variety and raw/unrefined foods. To study the impact of Cooking Anatomy Academy, we will take a qualitative approach and use journal entries to collect data. Participant journal entries will answer prompts like, “based on what you learned today, what will you have for a snack tomorrow,” or, “how many fruit servings should you have each day?” CAA mentors will record any positive or negative feedback they receive during each lesson. From the data we collect, we hope to see that CAA is helping participants make healthy food choices and increasing their obesity awareness.

Kurt Williams, Brigham Young University Life Sciences Macrophages play an important role in innate and adaptive immune responses, inflammation, and tissue repair and are characterized by two distinct phenotypes: classically-activated (M1) and alternatively-activated (M2) macrophages. M1 macrophages are characterized by a pro-inflammatory phenotype and are involved in production of pro-inflammatory cytokines and aggressive engulfment, whereas M2 macrophages are characterized by an anti-inflammatory phenotype and are involved in production of anti-inflammatory cytokines (e.g. IL-10) and tissue repair. Macrophage engulfment of apoptotic cells leads to polarization toward the M2 phenotype and is thus “immunologically silent”. Additionally, there is evidence that tumor-associated macrophages (TAMs) tend toward an M2 phenotype and as a result offer protection from an immune response in the tumor microenvironment. To further investigate the role of necrotic and apoptotic cells in regulating macrophage polarization, we cultured human macrophages with necrotic, apoptotic, or standard viable Raji cells and fluorescent beads and performed an engulfment assay. In a preliminary study we found that macrophages cultured with apoptotic cells showed a decrease in engulfment levels compared to macrophages cultured with necrotic cells. Macrophages cultured with standard viable Raji cells had the lowest levels of engulfment compared to macrophages cultured with apoptotic cells or necrotic cells. Thus, in our initial experiments macrophages cultured with necrotic cells appear to have a more “M1” phenotype, whereas macrophages cultured with apoptotic cells appear to have a more “M2” phenotype. Further experiments are necessary to validate this preliminary data and further characterize the capabilities of necrotic and apoptotic cells to differentially polarize macrophages. If these observations are replicated, it has potential applications in cancer biology and therapeutics, atherosclerosis, diabetes, autoimmunity, and other diseases with an inflammatory component.

Austin Pearce, Brigham Young University Life Sciences Agave utahensis acts as a keystone species across its native range in the Mojave Desert and Colorado Plateau (Gentry, 1982). As a keystone species, Utah agave contributes to soil formation along barren mountain ridges, and has provided starch-rich sustenance to Native American tribes. Furthermore, taxonomists consider each of the two subspecies, kaibabensis and utahensis, to have considerable morphological variation (Gentry, 1982) within their own unique ecological niches. Given the importance of Utah agave, the high degree of variation, and its unique ecological niches, there is surprisingly little information published regarding its physiological ecology. In fact, no effort has been made to determine the population densities of Utah agave due to the remoteness of the region and its difficult terrain (e.g., the Grand Canyon). Therefore, geospatial analysis tools specific to environmental niche modeling provide a powerful means through which these issues and knowledge gaps can be effectively addressed. My goal is to develop a species distribution model by joining known locations of Utah agave with climatic and environmental data in MaxEnt and ArcGIS software. Such a model can be used by others for further ecological field studies of Utah agave and its subspecies. Additionally, the approach I employ can be used as a pattern for mapping distributions of other important plant species in remote and difficult-to-access regions of the world.

Eric Middleton, University of Utah Life Sciences One of the most impressive examples of homing among birds is demonstrated by Columba livia, or the rock pigeon. In racing or carrier breeds, birds can routinely travel over 100 miles and reliably find their way back to their home loft (Pratt, 1954). Bred from feral rock pigeons, these racers and carriers have been selected for their increased ability to home. While much research has been conducted on the mechanisms of homing, the heritability of homing is not very well understood. Furthermore, it is unclear if homing is predominantly learned or innate (Melhorn, Haastert, Rehkamper, 2010). The existence of homing breeds demonstrates that homing is heritable, but the degree to which this is the case or what traits in particular are improved with selective breeding are more uncertain. To attempt to better understand the heritability of homing, we will compare the homing ability of pigeons who are siblings to each other, and to unrelated pigeons. We first captured 90 wild pigeons from areas around Salt Lake City and allowed them to breed freely. We tracked the ancestry of all of the pigeons born from the feral birds and banded each of the birds in order to reliably distinguish which birds were related. We are now in the process of teaching the captive bred birds to home. Once this is completed, we will release the birds at varying distances from the loft. To quantify homing ability, we will measure angle of displacement from the loft when the birds first orient themselves and being homing, the time it takes to home, and which birds successfully make it home to the loft. This data will then be used to compare the homing ability of siblings to unrelated pigeons.

Laurin Wilson, Utah State University Social and Behavioral Sciences Premature birth and subsequent hospitalization of an infant in the Newborn (or Neonatal) Intensive Care Unit (NICU) can be an extremely stressful time for parents and has been associated with maternal depression, anxiety, and decreased coping ability (Hack, Taylor, Klein, & Mercuri-Minich, 2000; Hughes, McCollum, & Sheftel, 1994; Partridge et al., 2005; Shaw, Sweester, St. John, Lilo, Corcoran, Jo, & Horwitz, 2013). Interventions to reduce parents’ stress levels during the hospitalization of their very preterm infants have mixed results (Boyce, et al., 2008; Matricardi, S., Agostino, R., Fedeli, C., & Montirosso, R., 2013). This study examined the medical, family, and maternal mental health factors that influenced feelings of parental distress for mother of very premature infants.

Daniel Cardoza, Brigham Young University Social and Behavioral Sciences This paper aims to offer an introductory platform to the correlation between Russian fatalistic tendencies and conversion to Protestantism. It argues that fatalism is a central aspect of the Russian collective conscious, something that is fundamentally at odds with the Protestant temperament as outlined by Max Weber in his book, The Protestant Ethic and The Spirit of Capitalism. What happens when a Russian converts to a Protestant religion? This paper specifically focuses on conversion to one religion, The Church of Jesus Christ of Latter-day Saints (LDS). Analysis of data gathered through ethnographic interviewing and participant observation shows that a correlation might exist between belief in LDS doctrines and a reduction of fatalistic tendencies. This paper serves an introductory basis for future research on this topic. As such, the paper also presents recommendations for further research.

Kyle Nelson, Brigham Young University Social and Behavioral Sciences Most research on eye movement while reading has focused on native speakers reading in their own language. This has led to detailed characterizations of the differences between the eye movement of English and Chinese speakers when reading. However, there is a lack of research concerning eye movement differences when reading in a second language. It is expected that English speakers, reading simple letters, would exhibit different eye movements when reading Chinese characters, where words are commonly made up of one or two intricate symbols. Similar adjustments in eye movement are expected if a Chinese speaker tried to read English. Our study seeks to first measure how second language skill influences eye movement during reading and secondly, to distinguish eye movement behaviors that are controlled by either cognitive or visual factors. We hypothesized that as individuals increase in proficiency in their second language, their eye movements while reading a second language would begin to mimic a native speaker’s reading his own native language. Participants were native Chinese or English speakers selected from Chinese language courses and ESL courses taught at Brigham Young University. Participants were matched based on language proficiency and history of exposure to their second language. Native Chinese and English speakers read paragraphs in both Chinese and English while an eye-tracking machine recorded their eye movements. We measured such variables as fixation duration, Saccade amplitude, refixation probability and other important variables. These results will be analyzed to find the differences in eye movement that occur when reading in a second language compared to the participant’s native language. The results, strengths and limitations of the study and future research will be discussed. We believe our research will be beneficial in characterizing how individuals read in a second language, assessing language proficiency, and directing the development of language instruction methods.

Dusty Pilkington, Weber State University Social and Behavioral Sciences Boundaries dividing political authority rarely match natural water flow. Multiple agencies, often with competing policies and agendas, manage water within single watersheds. This discrepancy can render management efforts ineffective. Local water agencies include soil conservation districts, local health departments, and engineering agencies. Agencies have varying funding sources, managerial structures, and jurisdictional boundaries. Fragmentation can frustrate management efforts at watershed scales. Comparisons between natural watersheds and human political geography can therefore be helpful in avoiding conflicts. This research examines discrepancies between political and physical geographies for sections of three Utah rivers : the Logan and Provo Rivers, and Red Butte Creek. These rivers are being targeted for intensive research as part of a statewide, multidisciplinary water sustainability project, titled iUTAH. Water-related land use (WRLU) data acquired from Utah’s Automated Geographic Resource Center were analyzed using ArcGIS geoproceessing tools. WRLU contributing to water quality in urban river reaches is described. WRLU was examined using three boundary sets, comparing the political geography of the river reaches, as depicted in municipal boundaries and management areas of community water providers, to their physical geography, as depicted in United States Geologic Survey Hydrologic Unit Code (HUC) watersheds. WRLU mixes using political infrastructure were contrasted with land use mixes from HUC boundaries. WRLU was classified in seven categories: residential, commercial/industrial, riparian/water features, irrigated agriculture, non-irrigated agriculture, farmsteads, and parks/open spaces. A gradient from rural Heber, to urbanizing Logan, to fully urbanized Red Butte Creek is shown. While Red Butte Creek HUC boundaries showed 53.8 % residential land use, Red Butte Creek municipal boundaries and community providers totaled 26 % and 39 % residential use, respectively. Differences emerged when irrigated agriculture was assessed using HUC boundaries. Irrigated agriculture totaled 0.2 % in Red Butte Creek, with Logan showing 29. 7% and Heber irrigated agriculture sitting at 41.1 %.

Renette Goodrich, Utah Valley University Social and Behavioral Sciences In the realm of Adoption most of the focus is given to the birth parents and the adoptive couple. However, there are many more people involved, some being the birth grandparents, who are the parents of the expecting parents. These grandparents, most of who are dealing with feelings of loss and grief, have been left to struggle through the experience by themselves. A scholar from Well’s, were the law requires social services to be offered to the extended family, conducted studies with grandparents; some of whom received social services and some who did not. His found a statistically significant difference in those who participated in the social services and those who did not. Grandparents who took advantage of social services where less stressed and more excepting of the adoption. This pilot study was conducted at one agency in Utah. Because of HIPPA laws the only access was to those grandparents who attended the support group. This study also showed social service support for these grandparents where significant; by lowering the stress level of dealing with a child’s unexpected pregnancy and helping them with direction and acceptance. This was a qualitative and quantitative survey handed directly to the participants to fill out and return. The agency where this survey was conducted and other associated agencies are interested in these results to better improve currents services and reach out to other grandparents who are not currently participating in these groups. This topic has not been studied in the United States; thus this study will add to the body of scholarly knowledge on this topic.

Ashley Jolin, University of Utah Social and Behavioral Sciences This paper analyzes the essentiality of creating a just European Union banking union between member states of the eurozone, in response to the European debt crisis. In 1992, the Maastricht Treaty established strict financial criteria for member states to follow, ensuring a strong economic environment to support the new currency union. However, numerous member states did not abide by these limits, and countries with weaker economic stability such as Greece and Ireland began to accumulate huge public deficits. As a response to this debt crisis, in June 2012 the key institutions and leaders of the EU proposed working towards a genuine Economic and Monetary Union, a banking union serving as the keystone of this project. I argue it is imperative that this banking union is designed to be successful at dissolving the current economic crisis and anticipating future problems, as the existence of the eurozone is contingent upon it.

Luz Maria Carreno, Utah State University Social and Behavioral Sciences Significance: Patients’ health literacy, or ability to comprehend and understand health information, influences their health status, knowledge about medical care and conditions, and hospitalization and adherence rate (Andrus, & Roth, 2002). Low-English proficiency patients are especially at risk, given they must overcome English and health literacy barriers.

Heather White, Brigham Young University Social and Behavioral Sciences The purpose of this poster is to illustrate the use of storyboards in visually evaluating exhibits and the visitor experience in a narrative museum. Storyboards, a key tool used to develop a visual story, can be used in museums to provide unique insights into the dynamic experience of visitors as they move through exhibits. This poster focuses on how storyboards can be used post-installation to evaluate how visual elements and space effect the visitor’s engagement and experience within exhibits. This poster will present storyboards for three exhibits and the resulting analysis. I analyzed the storyboards by examining artistic elements (line, color, shape, space, etc.) and design principles (unity, balance, scale, proportion, etc.) as they are formed and reformed throughout the storyboard. These changes are only noticeable because of the temporal aspect of sequenced storyboard images. The results show how the visual and spatial visitor experience encourages or discourages engagement with the exhibits. These results can be used, in addition to written and oral evaluation methods, to critique and improve exhibitions to be more accessible and engaging for future visitors.

Jason Lefrandt, Brigham Young University Social and Behavioral Sciences Dominant behaviors frequently occur in both human and non-human primates. In the non-human primate world, high and low dominance rankings are rated through interactions recorded within the group, specifically, aggressive and submissive behaviors. Dominance within Rhesus macaques (Macaca mulatta) also tends to be governed by a matrilineal effect: meaning that if two monkeys are in the same family, than both monkeys will show similar rankings within the group. This current study looks into how genotypic variables correlate with overall behavioral expression in dominance. Utilizing archived dominance and genetic data from the National Institute of Health including serotonin, MAO-A, and rearing, we analyzed the correlations with social dominance rankings of rhesus macaques. In order to control for matrilineal effects within established groups, all subjects were placed in smaller, newly formed social groups. This was done in order to facilitate great analyses of genetic influences on dominant behavior. Our original hypothesis was that macaques with long homozygote serotonin for the long allele; were mother-reared; and have the MAO-A gene will have high social dominant rankings. On the contrary, our preliminary findings have shown that subjects who were reared by mothers who were not genetically their own tend to show more dominant related behaviors, and are subsequently rated much higher than those subjects who were mother-reared or peer-reared.

Wendy Hendley, Utah Valley University Social and Behavioral Sciences The Personal Responsibility and Work Opportunity Reconciliation Act (PRWORA) passed in 1996 changed welfare dramatically. Meant to stop welfare fraud and dependency, it put into place numerous restrictions and promoted work, which meant that people on welfare, usually single mothers, were forced to participate in job-search activities in order to qualify for benefits. As a result, welfare recipients’ opportunities to attend college have decreased. Research has shown a negative correlation between educational attainment and poverty, the higher a person’s education the less chance they have of living in poverty. The human capital theory proposes that investing in education increases labor market returns. Unfortunately, with PRWORA’s focus on work, welfare recipients are forced to find low-wage jobs, which is not enough to lift them out of poverty. Although numerous studies have focused on welfare recipients’ reduced chances of receiving an education, few have looked at the wishes of welfare recipients themselves. This quantitative descriptive study uses secondary data analysis from a study done by the University of Utah’s Social Research Institute in 2006, and answers the following three questions: (1) what is the current level of completed education of welfare recipients; (2) do welfare recipients have a desire to further their schooling; (3) what barriers do they feel are preventing them from achieving their goals? This study found that a high percentage of welfare recipients had a desire to further their schooling, with the number one obstacle being financial. There is a significant gap between the wishes of welfare recipients concerning higher education and what is actually happening in their lives.

Jolysa Sedgwick, Brigham Young University Social and Behavioral Sciences Traditionally the Hmong in Thailand have made their living through subsistence farming and poppy cultivation. This arrangement had placed familial ties and religious influences at the center of Hmong cultural identity. Today, however, increased globalization has also meant a shift in cultural identity and priorities for the rising Hmong generation. This change is clearly seen in Nan Province where the decision to migrate or relocate to urban centers (such as Chiang Mai) for work is frequent. In the past, cultural identity and life decisions were heavily tied to kinship and religious influences. However, with the onset of globalization, the Thai State and current popular culture are becoming increasingly important to Hmong cultural identity and are becoming increasingly higher priorities in the lives of the Hmong who leave their childhood villages to pursue economic advancement and other activities in urban centers. In order to investigate this cultural shift, I conducted an ethnographic field study among Hmong living in three rural villages in Nan Province and among Hmong individuals in Chiang Mai. Using ethnographic field methods, I collected and analyzed the stories of those who decided or are in the process of deciding to relocate to Chiang Mai. In this paper, I present findings that support a shift in cultural priorities for those participating in urban migration and how that shift is influenced by increased global technology and connectivity. I explain that this shift is integral to understanding what the future will hold for the minority Hmong population in Thailand.

Danielle Jennings, Utah Valley University Social and Behavioral Sciences The popularity of surgical cosmetic procedures continues to climb in the U.S. (ASPS, 2012) and in other industrialized economies (ISAPS, 2011). This includes women in conservative cultures like Morocco and Afghanistan (Women of Afghanistan, 2012). This research was undertaken to examine attitudes towards plastic surgery among women who are members of the LDS Church. Utah boasts one of the highest concentrations of plastic surgeons in the U.S. (Lim, 2011) and is also majority LDS (Phillips and Cragun, 2011), rendering it ideal for this study. Data regarding attitudes towards surgery and levels of religious participation were collected from LDS women [n = 100] and men [n = 25] in Utah through a pilot survey administered in spring, 2013. The sample included women 19 — 81 years of age, 64% of whom were married, and 63% had grown up in Utah. Results indicated levels of religious participation were in keeping with other research involving Mormons (Hill et al., 2008) and 67% of women knew a female member of the Church who had had cosmetic surgery. Attitudes towards surgery varied by age and marital status. A second survey was created and administered via Qualtrics to LDS women [n =473] and men [n = 116] across the U.S. Results suggest variation in attitudes by geographic location, along with variation by gender, age, marital status, and familiarity with surgery. These results suggest aspects of Mormon life in Utah contribute to the popularity of surgery among Church members. These may include the pressures of dating and earlier ages at marriage (Maffly, 2010), coupled with demographic changes (Cragun and Phillips, 2011), as well as earlier ages at first birth of children (Davidson, 2008).

Kent Hinkson, Utah Valley University Social and Behavioral Sciences Post-traumatic stress disorder (PTSD) and depression both have individually strong relationships with academic performance and achievement. However, when considered together, the combination is associated with worse measures than either condition alone. Recently it has been suggested that student veterans may have elevated rates of PTSD and depression, which makes these conditions points of particular interest when looking factors that affect achievement and performance in school.

Lindsey Fields, Brigham Young University Social and Behavioral Sciences For my senior thesis in anthropology, I conducted fieldwork in a small Hmong village in northern Thailand studying the process of religion conversion. Traditionally, Hmong people practice a mixture of spirit rituals and ancestral worship known as Dab Qhuas and classified as shamanism. Within the past fifty or so years, other religious groups have emerged in the village both Christian sects and Hmong messianic groups. Though many in the discipline of anthropology argue that conversion is a break from traditions of the past, I observe that Hmong of this village in Thailand undergo a much more syncretic type of conversion. Though they identify as either Christian or of a Messianic sect, much of their practices and ideology retain elements of their shamanist past. Though they stress their differences in doctrine and practice, much of the ontology permeating shamanism and Hmong culture is still very much present. In order to better examine this phenomenon, I will focus the beliefs and practices in both Is Nbis and Christianity that take on slightly different forms in each religious practice, yet, still maintain some of the same ideologies. Rituals merely shift in order to fulfill the same metaphysical needs. By studying new practices that retain much of the feel of old culture rituals, I hope to illustrate that these Hmong are not as far removed from their traditional roots as they imagine themselves to be.

Mitchell Reid, Utah State University Social and Behavioral Sciences Chronic childhood aggression can be the beginning of a developmental trajectory that begins with minor aggression, moves on to physical fighting, and then to violence (Loeber & Farrington, 2000). Behaviors during toddlerhood may be more malleable than later in childhood (Reid, 1994). To better understand what predicts children’s early aggressive behavior we examined the role of parenting behaviors, parent mental health, and child characteristics in a sample of toddlers. Sixty-five mothers and their toddlers between the ages of 17 and 24 months (60% female) participated in the study. Mother-child dyads were videotaped during teaching and clean-up tasks. Mothers also responded to several questionnaires to assess toddlers’ social-emotional behaviors, language development, attachment security, and temperament and their own parenting stress and depression. The teaching and clean-up tasks were coded for maternal language supporting behaviors such as asking questions and providing praise, directives, expansions, and labeling. We examined the correlations between the independent maternal (depression, parenting stress, and language supporting behaviors) and child (age, gender, language development, temperament, and attachment security) variables and the dependent variable of aggression/defiance. Correlations between attachment security (r = -.28, p =.03) and maternal directives during the teaching task (r = .33, p =.01) and during the clean-up task (r = .39, p =.00) showed statistical significance. We included these variables in a regression model and found that attachment security and maternal directives during the teaching and clean-up tasks accounted for 29% of the variance in maternal reports of toddlers’ aggression and defiance. These results suggest that toddlers with greater attachment security and with mothers who use fewer directives in everyday tasks are rated as less aggressive and defiant than those with less attachment security and with mothers who use more directives. The full regression model and early intervention implications will be presented.