2014 Abstracts

Emma Penrod, Brigham Young University Communications A newspaper is the catalog of a community’s past. I spent about three years researching the impact of industrialization on Tooele from a variety of angles, primarily by reading archived newspapers from 1905 to 1970. During this time, I also collected and scanned nearly 200 original photographs. Industrialization came to Tooele in force in 1908, with the construction on the International Smelter and the Tooele Valley Railroad. Prior to the railroad and the smelter, Tooele was a small, predominantly Mormon community with an agrarian economy. Construction of the railroad, the smelter, and several affiliated projects could have employed 72 percent of every man, woman and child living in Tooele City in the early 1900s. An influx of immigrants changed the social landscape dramatically, reshaping Tooele as one of Utah’s most diverse communities. Through the early 1900s, conflict between the original Mormon settlers and the transplants embroiled the community in something of a perpetual identity crisis. Temporarily, a sort of physical segregation solved the problem-Mormons lived west of Main Street, and the immigrants set up shop east of Main Street. But it wouldn’t be long before the Tooele newspapers came to accept the newcomers and the advertising revenue they had to offer. The Great Depression and football, of all things, finally brought the community back together, and throughout the 1940s and 1950s, the immigrant families were invited to fly their native colors at important community events that celebrated Tooele’s heritage.

Sydney Tycksen, Utah Valley University Communications An award winning research project, this summary highlights research conducted by Communication students at Utah Valley University of a grassroots public relations campaign created to increase awareness among middle school students, parents, educators, and community leaders of the serious short and long term consequences of youth bullying. The study also demonstrates how the campaign informed audiences of the steps they should take to help prevent bullying. The goal of the research was to understand the short-and-long term effects of bullying, types of bullying, and the prominence of bullying in the surrounding community where the research was conducted. A public relations strategic communication plan was developed based on the research to change bullying behavior in the community. The campaign aimed to spread awareness of the short and long term effects of bullying and outline the steps to report and prevent bullying through the development of strategies and tactics. These steps help students, parents, educators, and community members understand bullying, recognize the signs and types, and know the appropriate authority to whom to report the behavior. This presentation will show how the research provided the students with the information needed to establish measureable objectives for the campaign, reach their key publics and provide an evaluation of the strategies and tactics used to meet campaign objectives. The document reviews the outcome and evaluation of each strategy and tactic implemented during the campaign, including media relations and social media tactics, The summary concludes with an overview of how campaign objectives of teaching key publics how to take steps to prevent bullying were achieved due to thorough research and understanding of how to effectively use messaging to generate behavior changes within the key public.

Emma Josey, Dixie State University Communications Romance novels seem to be a staple in the devoted reader’s array of books and have remained an extremely popular genre throughout the centuries. Not only are the actual stories appealing to the readers, but some readers go a step further and interact with or create a relationship with characters themselves. A phenomenon called parasocial interaction. This research study seeks to discover if the parasocial interactions female romance novel readers experience relate to a practice of a particular attachment style and jealousy in their romantic relationships. These characteristics are important to the health and sustainability of people’s romantic relationships, and this study seeks to learn whether or not the quasi relationships romance novel readers create with characters influences those relationships.

Indigo Klabanoff, Dixie State University Communications With globalization and the minority population changing, it is extremely important to learn the real statistics that are out there when it comes to single parenting. The most important thing though, is to figure out is which communication styles work in healthy communication, with single parent families.

Michelle Fordham, Utah Valley University Education A Family Write Night is an opportunity for teachers, students, and parents to come together to learn about the different stages of writing development and what writing does for the student at any level. Since writing will strengthen and enhance the student in other literacy skills and knowledge, bringing these students and families together in a friendly, casual atmosphere to provide enjoyable, non-assessed activities builds confidence in a writer’s sense of who they are, their voices as writers, as well as developing critical thinking skills. Parent and family involvement is a key component of this activity. Having families, students, and teachers working together to encourage writing at home builds strong family bonds while strengthening writing skills.

Chelsey Funk, Utah State University Education High school English as a Second Language (ESL) students often feel separated from their schools and communities. These feelings of separation can lead to low engagement and low achievement despite the students’ desire to do well in school. One method used to counter low engagement in mainstream classes is service learning, but there is little research on service learning with ESL students. In this study, an existing group of 9th grade ESL students was taught and observed to determine the feasibility and effectiveness of using service-learning methods. The project endeavored to tie academic work to community involvement and therefore increase student engagement and academic confidence. Throughout the implementation of the service-learning project, students displayed increased classroom involvement and reported improvements in attitudes and self-assurance. The methods and procedures described in this study can be adapted to larger class sizes and a variety of projects in order to increase engagement and academic confidence in ESL students.

John Lorz, Utah Valley University Education How about a 20% increase in the class average on the mid-term exam compared with the previous two academic years of the same course? In this session two students share their experiences as (newly piloted) peer mentors in a low-performing, upper division genetics class. Student attitudes towards genetics improved and both mid-term and final grades increased by double-digit percentages. Not only did these peer mentors increase student success, but also experienced a personal developmental opportunity in preparation for graduate school, including this conference presentation and an anticipated peer-reviewed publication to follow.

Stone Samantha, Utah State University Education The present study investigated language growth over the course of 3 years for 50 Spanish-English Bilingual children who were learning English as their second language. Children were asked to retell a story in English and in Spanish at 6 time points (before and after Kindergarten, First, and Second Grades). The stories were transcribed and utterances were coded as to whether they contained mazing behaviors such as revisions, false starts and corrections and also coded for the level of syntactic complexity (simple or complex) they contained. Simple sentences contained one main verb, and complex sentences contained two or more main verbs. The presence of mazing is thought to indicate hesitation or difficulty in lexical retrieval and might be expected to occur more in complex than simple sentences. Further, we hypothesized that children would evidence more mazing behaviors in their non-native language (English) than their native language (Spanish). Ultimately, we theorized that children’s use of mazes in English would decrease over time, as they became more proficient in their second language (English). Some of the children were receiving instruction in schools classified as “English Immersion” models, and others were in schools that incorporated a “Transitional” approach to instruction. Results are discussed in terms of current theoretical models of bilingual language acquisition and variations in outcomes as a function of current instructional models.

Skylar Larsen, Utah Valley University Education In science education, greater learning gains have been associated with engaged learning strategies over traditional lecture formats. This approach facilitates development of higher-order cognitive skills (HOCS; critical thinking and application of knowledge) which are not only imperative for all successful scientists but necessary for every citizen functioning in our society. Development of these HOCS, however, is not accomplished in some classrooms due to lack of alignment of learning objectives and evaluation, or lack of assessment of these skills entirely. Specifically, we asked how do learning goals and objectives align with assessments, and are learning gains on HOCS better achieved by students in more aligned classrooms tested with higher-cognitive level assessments?

Tyler Mathis, Brigham Young University Education We need to improve our educational system through giving our teachers more chances to be given constructive feedback through teacher evaluations. As teachers receive feedback and are put through effective teacher evaluations their performance will improve and the upcoming generation will be more educated. This will greatly beneficial to the future of our society as a whole. Teacher evaluations is an important topic to raise awareness of to help produce effective and beneficial teaching to the upcoming generation. The topic I will address is how we can make teacher evaluations more effective by exploring ways to quantitatively measure teachers’ effectiveness through examining student gains. I will also examine why implementing teacher observations will be an asset to teacher performance and a necessity in teacher evaluations.

Kristin Murphy, Utah State University Education Past research shows that parents interact more positively with their children and use more supportive language during play than during teaching interactions (Kwon, Bingham, Lewsader, Jeon, & Elicker, 2013). Children with normal language development tend to have parents who use more language supporting speech, (Vigil, Hodges, & Klee, 2005) than parents of children with language delays. The specific research question addressed in this study is: Do maternal (maternal depression, education levels, parenting stress) or child factors (language development, social-emotional development) influence maternal language behaviors in teaching and play contexts?

Jonathan Welling, Brigham Young University Education The demographic shift of Mexican immigrants within the public school system in the US has created a great need for teachers to better understand the expectations that immigrant families bring with them from their education system in Mexico. This research provides contextual information to educational stakeholders in the US to better accommodate to the needs of immigrant students from Mexico. This study explores the norms, roles and expectations of teachers, parent and students in the education system in Irapuato, Mexico. Data was collected from observations and interviews with teacher, parents and students from three different public high schools in Irapuato, Mexico: CBTis- a technical school, The Official Preparatory School of Irapuato- a standard public school and SABES- a small rural school. Analysis was done using a systematic approach of open coding to identify emergent themes. Preliminary results provide contextual information explaining the expectations parents hold for the teachers in Irapuato, Mexico. The data is valuable in understanding the norms, expectations and perceived roles held immigrant Mexican families. Applications of this data may be used to facilitate both collaboration between teachers and Mexican immigrant parents and the adjustment of immigrant students to a new educational system.

Shawnie Cram, Dixie State University Education English Language Learners (ELL) are confronted with many challenges such as insufficient language proficiency, lack of content knowledge, and adjusting to a new culture. Classroom teachers make efforts to effectively work with these students so they can become a vital member of the classroom community. However, if there is only one teacher in a classroom, they often do not have the time to assist the ELL at an adequate level. Using the participatory action research approach, this paper examines the ways in which a pre-service teacher can assist the classroom teacher by clearly identifying the goal for the ELL student. The goal set by the classroom teacher for this *ELL student is to reach grade level reading competency by the end of the academic school year. Through comprehensive discussions between the classroom and pre-service teacher, the methods and strategies based on Second Language Acquisition theories were decided to be implemented as tools of instruction. The pedagogy of reading across curriculum in a kindergarten classroom is utilized as the basic mode of instruction as the pre-service teacher and the classroom teacher collaboratively use different methods and strategies. The current data of the action research indicates that in three months, the ELL will reach approaching grade level proficiency with the assistance of the pre-service teacher. By the end of the academic school year, the author anticipates the ELL will demonstrate his reading competency at grade level.

Shadlan Gale, Utah Valley University Education This paper presents ongoing research comparing the learning performance of students using online and electronic textbooks/course content (e-text) for business-related classes versus students using traditional, hard-copy textbooks. In fall 2013, the Woodbury School of Business (WSB) at Utah Valley University (UVU) converted over fifty courses to e-text only. The WSB made this transition in many of its classes for various financial reasons as well as to provide students with a more interactive way of learning course material through the application of the additional learning tools that can only be found within the e-text platforms. In a previously published paper, the authors showed that no statistical difference was found in test scores for students at UVU taking a class which implemented the use of e-text among three different delivery types: live, hybrid, and online. This paper focuses more on discovering if student performance and test scores change by using e-text versus hard-copy textbooks and not in relation to the delivery type of the course.

Shadlan Gale, Utah Valley University Education This paper presents ongoing research to examine faculty experience and attitudes toward the implementation of electronic textbooks/course content (e-text) for college courses. In fall 2013, the Woodbury School of Business (WSB) at Utah Valley University (UVU) converted over fifty courses to e-text only. The WSB made this transition in many of its classes to reduce costs and provide students with more interactive course materials through additional learning tools found within the e-text platforms.

Christopher Green, Utah State University Education Statics is a gateway engineering course. Many students use their performance in a statics course to evaluate and judge their desire to continue within an engineering field. Students’ performance can be adversely impacted by misconceptions they may have regarding class content and analysis techniques. Feedback from instructors can help students navigate through their misconceptions. It is critical that this feedback be concise and timely to prevent a slip in self-efficacy, or an increase in their frustration. Both factors can negatively impact a student’s desire to persist in engineering. Implementation of online learning logs allows students a timely communication avenue that can reveal to the instructor indications of such factors. This study is designed to explore common misconceptions exhibited through learning logs in a pre-professional engineering statics class. Ninety student logs were consensually evaluated in a fall 2013 statics course delivered at Utah State University. A “discourse analysis” technique was used to review learning log data to discover students’ trouble areas within the class. Results indicate typical stumbling areas that students encounter in a statics course and allow insight into specific instruction areas that need to be refined to better deliver content to the class as well as individuals.

Jessica Hill, Utah Valley University Education The transition to junior faculty member can be difficult for graduate students (Sorcinelli, 1992). Despite significant teaching requirements for most assistant professor positions, many graduate programs minimize teaching experiences. Due to this climate, the developmental process of novice graduate instructors is poorly understood (McKeachie & Svinicki, 2010). We investigated the utility of the Teacher Behavior Checklist (TBC), a measurement tool developed by studying master teachers (Buskist et al., 2002), as a means to evaluate classroom performance of first-time and novice graduate instructors (GIs).

David Turner, Utah State University Education Problem based learning is an approach to education where students develop solutions to authentic problems (Hmelo-Silver, 2004) with support from scaffolding (Wood, Bruner, & Ross, 1976; Reiser, 2004). Computer based scaffolding helps students organize thoughts and arguments while solving problems (Belland, Glazewski & Richardson, 2008). We examined how students from one small group constructed order in their interactions and arguments as they solved an environmental issue using a stakeholder lens.

María Velasco, Utah Valley University Agriculture Calochortus is a plant genus that includes approximately 60 species distributed in North America with great ornamental and ethnotobanical value. Sego lily, mariposa lily and other common names have been used to describe the different species of Calochortus. Native Americans and settlers have used the bulbs as food, either raw or ground into flour and cooked. C. nuttallii played an important role in the success of colonization of Utah. Between 1840 and 1851 the scarcity of food due to a plague of crickets led to the pioneers to dig for the bulbous roots of sego lily, ensuring the survival of the pioneer population. This research studies the effects of Gibberellic acid on the germination and elongation of these species in vivo and in vitro. Eight different species of Calochortus are being utilized for this experiment to evaluate the effect of this growth regulator on the germination of these species. Different types of media are being tested to evaluate the optimal in vitro conditions that these species require for germination and growth. In addition embryogenesis and organogenesis is being induced by using Benzyl Amino Purine and 2,4-Dichlorophenoxyacetic acid at different concentrations. Sego Lily is the State flower of Utah; micropropagating this plant will allow us to understand and research new methods and conditions to grow these species successfully, making preservation possible since some Calochortus species have been listed as endangered. In addition, other species such as Calohortus ambiguous (Doubting Mariposa Lily), which is distributed in Utah, Arizona and New Mexico, will also benefit from the system developed in this research because they are closely related to Sego Lily (C. nuttallii). The long term goal of this research is to establish an appropriate micropropagation system for a wide variety of Calochortus species.

Sarah Dursteler, Weber State University Communications The culture of sex outside intimate relationships including dating scripts and sexual scripts has shifted to one of more ambiguity. Multiple changes in sexual scripts have occurred in the last 50 years with the diversification of types of relationships in which sex occurs, including, a greater acceptance of sex outside of relationships (Gagnon & Simon, 1987). This study is in response to the call for more research on how social identities influence perceptions of sexual interaction (Backstrom, Armstrong, and Puentes, 2012). The purpose of this study is to examine hook-up culture outside of traditional intimate relationships. This study explores the extent of the shift from traditional dating scripts to a culture of hooking up as perceived by males and females that identify with the predominant Latter-Day Saint (LDS) religious culture. Using the tenants of social exchange and scripting theories this study examined the attitudes of males and females. Transcripts from four same gender and religion focus groups and 100 open-ended survey responses provided data for analysis. The constant comparative method (Glaser and Strauss, 1967) provided the means by which to analyze data within emergent categories. One open-ended response question was reviewed to assess content theme analysis. The constant comparative method indicated distinct contrast of perceptions of sexual intimacy between males and females. Findings suggest that there are distinct differences between male and female and LDS and non-LDS attitudes about and perceptions of hook-up culture. These results are consistent with previous research indicating that hooking-up can be a functional strategy used to shift focus from traditional intimate relationships to more academic and professional goals.

Taylor Topham, Dixie State University Communications With the new generations comes new forms of entertainment. Rarely is there a home without a television. The Cultivation Theory presented by George Gerbner and his colleagues states that television is so common in our society that it has an affect on our perceptions of the world. The Cultivation Theory specifically looks at violence on television and its effects on the viewer. Those that are heavy viewers of television often have what is known as mean-world syndrome. Because of the violence they watch on television, they are more likely to see the world as a violent place (Littlejohn & Foss, 2011). Along with a television often comes some type of video gaming system. Studies state that over 65% of North American households now have a video gaming system (Chiawen, Aiken & Huang, 2012). The purpose of this research is to determine if video gaming effects the perception of individuals similarly to that of television as described by George Gerbner’s Cultivation Theory.

Jessica Read, Brigham Young University Communications As the world of online social networking has changed to accommodate public interest, online dating sites have become increasingly popular. With this new trend in social media there has been an increased awareness as to how one might present him or herself in the most favorable way possible through computer mediated stimuli. To find out if nonverbal cues played a significant role in online impression formation, we had males and females randomly assigned to one of two variables, where they viewed stimuli of a member of the opposite gender. The stimuli that was presented was either a photograph accompanied by a written autobiography about a member of the opposite gender, or a video of the person reading their own autobiography, which allowed for the presence of nonverbal cues. Participants rated the subjects in the assigned stimuli on a number of different scales ranging from trustworthiness to sexual attractiveness based on their first impressions. Results showed no significant differences between the presence of nonverbal cues in the video stimuli or the photographic stimuli, reasoning for the indifference participants had towards the nonverbal cues is discussed. However, there were significant gender differences among the first impressions formed, other findings are presented.

Lee Leavitt, University of Utah Life Sciences The ventral respiratory column (VRC) is a region in the brainstem shown to control breathing patterns in mammals. Using activation and inhibition of neurons in this region, classes have been assigned based on response-combinations. Using a mouse model, cells from this region are dissociated, plated and incubated with a dye that indicates changes in cytoplasmic calcium levels. Hundreds of cells are measured while varieties of pharmacological agents are applied. Response-combinations provide a profile of the receptors found on these neurons. Previously, varieties of cell classes were shown to contain NMDA receptors (receptors linked to learning and memory). However, specific compositions of subunits within these receptors are not known. These receptors are ligand gated ion channels composed of four non-covalently bound proteins. Each subunit has a different activation profile determined by interactions of agonist and antagonists. Conantokins (peptides isolated from snail venom) and other compounds further afford understanding of the architecture the assigned cell-classes. This project has continued to classify the subunit compositions of NMDA receptors with the ultimate goal of understanding which NMDA receptor subunits are present in each class. This will provide valuable information on the VRC’s function, and will allow for pharmacological innervations to change behavior in this region.

Dasom Kim, Brigham Young University Life Sciences The main purpose of the project is to compare the phylogeography of a species of stonefly (Klapopteryx kuschelli) and a species of dragonfly (Rhionaeshna variegata) in Patagonia. Specifically, the project will study how geography and behavior (i.e., their dispersal abilities) have affected their evolutionary histories. Of all the varied climates and geography in South America, comparatively few phylogeographic studies have been conducted with insects, especially in Patagonia. This study will provide an important foundation for a comparative phlyogeographic study of two insect groups inhabiting the same regions of Patagonia. Also, if funded, this project will give me an exclusive opportunity to interact with international research institutions in South America as well as their scientists.

Madison Landreth, Weber State University Life Sciences Enterococcus, a bacterial genus that normally inhabits the gastrointestinal tract of animals, can be pathogenic to humans, causing urinary tract infections, sepsis and other serious diseases. It is also one of the major causes of hospital acquired infections. One important complication of those infected with Enterococcus is the fact that these bacteria often have a high level of antibiotic resistance, making effective treatment of patients more difficult. While Enterococcus is a normal inhabitant of the gastrointestinal tract, it can survive outside its host in the environment, even in adverse conditions, such as the Great Salt Lake (GSL). In this experiment, hundreds of isolates of Enterococcus were collected from the Great Salt Lake, from various sites along the Weber River which flows into the GSL and from clinical sources. Isolates were tested for different phenotypic characteristics and for their resistant patterns against certain antibiotics. Preliminary results of the Kirby Bauer disk-diffusion assay demonstrated that 47% of enterococcal isolates from the Great Salt Lake were resistant to one or more of the five antibiotics compared to 98% of the clinical isolates. In contrast, in a previous study, as few as 15% of Enterococcus isolated from the fresh water sources were resistant to one or more of the five antibiotics. These data may have implications concerning the importance of anthropological impact on rates of antibiotic resistance in this genus.

Derrick Crawford, Brigham Young University Life Sciences Members of the FGF family of signaling factors are key components in distal outgrowth and patterning of the vertebrate limb. These factors are expressed and secreted by the apical ectodermal ridge (AER) on the distal margin of the limb. Blocking their function is known to truncate the limb skeleton. Conversely, replacing the AER with beads soaked in Fgf protein can rescue limb outgrowth and patterning. Our lab has demonstrated that one of the mechanisms whereby the Fgf/AER functions is to mediate directed outgrowth of the adjacent mesenchyme. As the AER regulates growth of mesenchyme toward itself, it would be predicted that the AER’s dimensions would be important for shaping the mesenchyme that it recruits. We have found that the shape of the AER changes over time in a manner that corresponds to the shape of limb elements as they form along the proximal distal axis. Further, mutants that exhibit defects in the dimensions of the AER show corresponding anomalies in the limb skeleton. Given these observations it would be predicted that an Fgf soaked bead being of fixed spherical dimensions would only be capable of forming a cylindrical, rod-shaped limb. A bead placed posteriorly fulfills this expectation whereas a bead placed apically does not. We provide a molecular explanation for this discrepancy. We have also manipulated the shape of the AER surgically and find that similar to beads the shape and the AP position of the AER dictates the shape of the forming limb skeleton.

Jake Gamboa, Brigham Young University Life Sciences It is estimated that approximately 350,000 people in the United Stated die annually from post-myocardial infarction arrhythmias. A majority of these people will undergo a surgery that results in partial or complete removal of the stellate ganglion and other nerve fibers of the pharyngeal space in an attempt to prevent over stimulation from the neurons to the area of dead heart tissue and, therefore, future arrhythmias. However, without a somatomototopy, it is unclear what physiological effects partial or full sympathectomies may have. We will create a three-dimensional map of the pharyngeal space nerve plexus which will, in turn, allow for a more accurate and precise surgery.

Teressa Paulsen, University of Utah Life Sciences Breast cancer is still the most common cancer among women regardless of race or ethnicity. The focus of our research is to uncover the mechanism breast cancer cells use to escape the inherent limitations of the telomere and obtain immortality. The protective end of a chromosome, the telomere, degrades with each cellular division. The cellular response to telomere dysfunction is to activate programmed cell death. Therefore, this type of damage normally limits the proliferative potential of the cell and subsequently carcinogenesis.

Josh Hall, Weber State University Life Sciences Reproduction in birds is extremely conservative with the vast majority of the birds adopting bird-egg contact incubation to maintain an appropriate microclimate for embryonic development (Deeming, 2004). The Great Salt Lake is a vital nesting site for American Avocets (Recurvirostra Americana) that shows extreme temperatures and hostile environments where nest success can be as low as 1 -14% (Cavitt, 2008). Constancy of incubation, i.e. the time that the eggs are in contact with an adult, is a major indicator of nest success and environmental conditions. Our goals were to examine some of the costs natural selection places on embryos and parents to maintain a constant embryo temperature. We hypothesized that incubation attentiveness would increase across the nesting cycle. Over 200 AMAV nests were surveyed. Thermal probes were used to record various nest microclimates at every minute. A pseudonest with painted chicken eggs was also created and a thermal probe was placed to measure the ambient temperature without any adult incubation. A motion sensitive camera was placed over nests to examine differences in parental care. Nests will be divided into three phases: early, mid, and late incubation. Thermal data will be analyzed using descriptive statistics and mean variance values to calculate how incubation constancy varied throughout these phases. We expect this data to tell us more on how natural selection is working on these populations and some possible theories of how this developed.

Goyeun Tun, University of Utah Life Sciences Chronic Fatigue Syndrome (CFS) and Fibromyalgia Syndrome (FMS) are disorders which their symptoms and treatments are not clearly known. CFS and FMS are not life threatening diseases; however, they can affect patients’ quality of life because they experience symptoms including exercise intolerance, need for bedrest, and debilitating chronic pain and fatigue with these disorders. The research from Dr. Light’s lab has shown that moderate exercise for 25 minutes causes changes in mRNA levels in CFS and FMS patients but not healthy controls. The objective of our study was to examine changes in white blood cell gene expression of CFS and FMS patients both on Lyrica and on placebo in a double-blinded, cross-over design (where each study subject was his or her own control) by using quantitative PCR gene expression analysis. The lab routinely analyzes blood samples for 48 different genes from study subjects and healthy controls collected before (baseline) and then 8, 24, 48 hours after exercise moderate exercise. My focus was on changes in expression of two ATP-responsive purinergic receptors, P2X7 and P2Y1, which have not been studied after exercise in CFS and FMS but have been associated with chronic inflammation and pain in animal models. White blood cell layers (buffy coat) were collected from samples, RNA was extracted and converted to cDNA. 384 well PCR plates were robotically loaded from 96 well source plates, then the PCR reaction was run in an ABI 7900 thermal cycler that tracks fluorescence in “real time” (real time qPCR). Analysis of results is in progress and will be reported on the poster.

Xin Wan, University of Utah Life Sciences Endothelial dysfunction exists in individuals with diet-induced obesity (DIO) and type 2 diabetes (T2DM). Markers of endothelial dysfunction include reduced phosphorylation (p) of endothelial nitric oxide (NO) synthase (eNOS) to total eNOS (p-eNOS:eNOS), and attenuated endothelium-dependent vasorelaxation. Free fatty acids (FFAs) are elevated in individuals with DIO and T2DM. Our laboratory has shown that when: (i) endothelial cells are incubated with saturated FFA palmitate; (ii) mice are infused with lard-oil; and/or (iii) when mice are fed with high-fat diet, protein phosphatase 2A (PP2A) binds directly with eNOS. When this occurs, the association among Akt-Hsp90-eNOS is disrupted, p-eNOS:eNOS is impaired, and endothelium-dependent dysfunction occurs. This is prevented using pharmacological and genetic approaches that limit production of FFA metabolite ceramide. It is unknown whether PP2A inhibition per se is protective. We hypothesized that arterial dysfunction in obese vs. lean mice is prevented by PP2A inhibition. Seven-week-old, male, C57B16 mice consumed standard (CON, n=20) or high-fat (HF, n=20) chow for 12-weeks. Subgroups (n=10) of CON and HF mice received IP injections of saline (vehicle; V) or Lixte Biotechnology 100 (LB1, 1 mg/kg/day) for the last 14-days. Preliminary experiments verified that LB1-treatment for 3 and 21 days decreases (p<0.05) arterial PP2A activity. HF mice gained weight and developed peripheral glucose intolerance vs. CON mice regardless of LB1 treatment. Endothelium-dependent vasorelaxation was impaired (p<0.05) in HF-V vs. CON-V mice, but dysfunction was less severe (p<0.05) in HF-LB1 mice. p-eNOS:eNOS was reduced (p<0.05) in arteries from HF-V vs. CON-V mice, but p-eNOS:eNOS was similar in arteries from HF-LB1 and CON-LB1 mice. Akt and Hsp90 co-immunoprecipitation with eNOS was impaired (p<0.05) in HF-V vs. HF-CON mice, but this was not observed in arteries from HF-LB1 and CON-LB1 mice. These findings suggest that PP2A activity suppression in vivo is sufficient to preserve endothelial function in obese mice.

Jacob Anderson, Utah Sate University Life Sciences Leafy spurge (LS) is an aggressive Eurasian forb that has been successfully reduced in many areas in western North America through the biological control releases of flea beetles. Long term studies of this phenomenon are sparse. Three flea beetle species were released in the mid-1990s at a site dominated by LS in Richmond, Utah. This study assessed the long term effects of LS biocontrol on an ecological community at this site by addressing five questions: (1) Is LS abundance significantly lower now than in the 1990s? (2) What plant species are replacing LS and are they native or non-native? (3) Have the flea beetle populations persisted since their initial release? (4) What part does soil type play in which flea beetle species now dominate at the site? (5) In response to their unexpected presence, what role may long-horned beetles contribute to the long-term reduction of LS? It was found that LS abundance has significantly decreased from the 1990s; the dominant plant species are those of non-native grasses; flea beetles have persisted in significantly smaller numbers, with Aphthona lacertosa being the most abundant; and long-horned beetles appear to play a significant role in the reduction of sexual success of LS. The results of this project have implications for land managers when considering the vegetative response to LS biological control and the importance of long-horned beetles for long-term in managed, LS-reduced habitats.

J David Symons, University of Utah Life Sciences Autophagy plays a central role in cellular quality control by destroying damaged or excess proteins, lipids, membranes, and organelles that accumulate in response to deviations from homeostasis. The existence and role of autophagy in endothelial cells (ECs) and blood vessels has not been established. Autophagy can be quantified by assessing the ratio of the membrane bound conjugate of microtubule-associated protein light chain 3 (LC3-II) to the cytosolic non-lipidated conjugate LC3-1 (LC3-II:LC3-I) or GAPDH (LC3-II:GAPDH) via immunoblotting. We sought to determine the extent to which a variety of cellular stressors induces autophagy in ECs and intact blood vessels. LC3-II:LC3-I or LC3-II:GAPDH was elevated (p<0.05) (i) 450±6% (n=4) in ECs incubated for 2 h in amino acid (AA)-deplete vs. AA-replete media; (ii) 47±3% (n=3) in arteries from fasted (14 h) vs. fasted / refed (1 h) mice; (iii) 40±2% (n=3) in arteries from mice that completed acute exercise vs. sedentary controls; (iv) 38±1% in arteries from exercise-trained vs. sedentary mice under basal conditions (n=2 per group); and was decreased (p<0.05) (v) 57±8% (n=4) in arteries from ~30 month-old (i.e., old) vs. ~6 month-old (i.e., young) mice. Further, indices of autophagy were elevated (p<0.05) 101±6% in ECs exposed to 3 h x 500 uM palmitate vs. vehicle (n=4), and 50±6% in arteries from obese vs. lean mice (n=4 per group). Thus, autophagy is altered in ECs and blood vessels in response to physiological (e.g., fasting, acute exercise, exercise training, aging) and pathophysiological (acute lipotoxicity, diet-induced obesity) stimuli. Ongoing research will determine the functional role of vascular autophagy in health and disease.

Janweb Lagazo, Brigham Young University Life Sciences The scientific community and the general public have long been interested in the effects of water pollution. Most studies on water pollution have focused solely on industrial pollution, but have failed to consider the potential impact of pharmaceuticals that unintentionally accumulate in aquatic ecosystems via wastewater treatment effluents. The purpose of this study is to advance our understanding on how these wastewater effluents affect aquatic ecosystems in Utah. We quantified the concentration of select pharmaceuticals in Hobble Creek using mass spectrometry. Then we sampled above the treatment plant, at the effluent outlet, and downstream of the effluent to determine pre-effluent and post-effluent drug concentrations. We are currently using this preliminary data to investigate how common endocrine disrupting, anti-inflammatory, analgesic, and anti-anxiety drugs may potentially affect the aquatic ecosystem of the endangered Chasmistes liorus, commonly known as June sucker.

Laurel Thompson, Utah Valley University Life Sciences Breast cancer is divided into subtypes which are defined by their proteomics, histology, and genetic expression profile. Current methods, therefore, are aimed at testing these, and include DNA microarrays, immunohistochemical staining, and proteomic analysis. These methods are effective classifiers, but are not easily transferable to real-time clinical applications, such as the determination of cancerous status during operation or when taking a biopsy. The determination of molecular subtype by other means would be a significant advancement in cancer detection and treatment. We have made some preliminary studies that suggest high-frequency ultrasound may be sensitive to variations among the cancer subtypes as manifest in cell cultures through their cytoskeletal protein structure, which has a distinct spectral signature. The object of this study was to explore the basis for this variation through a combination of experimental and theoretical analysis. We used first-principal modeling methods and compared the model spectra generated from these to spectra obtained in the cell culture lab. Variations in bulk modulus, cell position and size were modeled and combined with experimental spectra in principal component analysis (PCA), and the Euclidean distances between each principal component of the experimental were found as they relate to the theoretical principal components. A graphical method similar to heat maps used for gene expression profiling was then developed to display the relative distances (similarities) between spectra. The program was tested by comparing experimental spectra of three breast cancer cell lines to model spectra. The results indicate the properties and thus molecular subtypes of breast cancer cells could potentially be determined by comparing their measured spectra to model spectra using a feature classification program such as PCA and that these classifying features can be displayed in a convenient graphical representation according to their spectral similarities.

Austin Spence, Utah State University Life Sciences This study investigated the physiological effects of localized habitat disturbances around two well-studied hibernacula of the wandering gartersnake Thamnophis elegans. After three years of monitoring snake morphology and physiology at several hibernacula, several disturbance events occurred, including log clearing, stream bank disturbance, and vegetation removal. Individuals from three populations, two with disturbed hibernacula and one control population with no disturbance, were collected during the spring emergence immediately following the disturbance. Blood samples were collected upon capture and following a uniform stressor to measure baseline and post-stress physiological conditions. The samples were analyzed using a radioimmunoassay to measure corticosterone levels and a bacterial killing assay to measure innate immunocompetence. Baseline and post-stress corticosterone levels were higher in both populations with disturbance events compared to the control population. The bacterial killing ability of the site with the most anthropogenic activity was lower than the control site, indicating immunocompromise. Data are currently being analyzed to assess differences within the same populations between years with and without disturbance events. Pre-disturbance data are a rare and useful commodity and allow us to facilitate a better understanding of the various effects of anthropogenic change on natural populations. This study was funded through the Undergraduate Research and Creative Opportunity Grant from Utah State University.

Dani Peterson, Brigham Young University Life Sciences Due to its long and complicated trajectory through the cranium, facial nerve VII (CN VII) can be damaged in surgeries, sometimes resulting in facial muscle paralysis. Surgical removal of acoustic neuromas and parotid tumors, in addition to surgical repair of the temporomandibular joint disorder are associated with a risk of damage to CN VII. In addition, insertion of auditory implants can damage the nerve, as can improper stimulation to the nerve after the implantation has occurred. We will create a three-dimensional (3D) model based off of data from dissection of the nerve in a human cadaver in order to give physicians a greater in vivo knowledge of the pathway of CN VII. We have dissected the lateral side of the right half of the head to the level of the parotid gland, identified the parotid plexus of CN VII, and followed its five branches. In addition, we are currently following the nerve through the internal auditory meatus on its pathway through the temporal bone. In preparation for the modeling MicroScribe technique described below, we have imaged the head using Magnetic Resonance Imaging (MRI) at BYU. These images will be used as a template for the nerve reconstruction model. After completing the dissection, we will track the nerve trajectory using a MicroScribe 3D Digitizer. The MicroScribe technique is used to create 3D computer models of any physical object. The user sets reference points and uses the stylus to trace data points of the object’s contours. Our final product will be a 3D spatial computer mapping of CNVII, as well as a mapping of the skull, parotid gland, and other landmarks to put the nerve model into context. We hypothesize that with our approach and MicroScribe technique, we will be successful in creating an accurate model of CN VII in the head.

April McMurray, Brigham Young University Life Sciences Diabetes Mellitus Type 2 (DMT2) is a lifestyle-related disease where the body does not produce enough insulin or the cells are unreceptive to it, and it is now the most common form of diabetes. Individuals who do not control the disease can suffer serious complications such as limb amputation, damage to the eyes, kidneys, nerves, heart, and it can be very costly. This problem is particularly serious in Tonga; the prevalence is almost twice as high as that in the United States. The purpose of this research project was to determine to what extent the cultural, economic, and educational factors contribute to such high prevalence. In May I traveled to Tonga with the nursing students from Brigham Young University to conduct my research. I distributed surveys to patients and medical staff in the diabetes clinic in the Vaiola hospital in Nuku’alofa, Tonga. The surveys had questions related to their socioeconomic status, understanding and attitudes of diabetes, as well as patient management practices. While I was there, I also kept extensive field notes on observations related to my research, which provided supplemental information regarding the Tongan lifestyle that was difficult to gather from the surveys. Preliminary analysis indicates that there has been a very small, positive shift in understanding and attitudes towards DMT2, but economic- and culture-based habits still impede Tongans from managing the disease effectively. There were several limitations to this study: small sample numbers, lack of resources, some resistance from Tongan medical personnel, and particularly cultural barriers made it difficult to gather enough information to come to significant conclusions. However, the research does give insight concerning potential future studies and interventions to help the people of Tonga treat this disease.

Jacob Bassett, Utah Valley University Life Sciences Many discoveries regarding the complex interplay between biological pathways within a cell begin with attempts to link new observations to scrupulously studied cellular mechanisms. Our lab is studying the soluble inter-mitochondrial space protein mdm35, which has been observed to facilitate the function of ups1 & 2 proteins, as they in turn regulate the mitochondria’s phosphatidic acid metabolism. In addition to this phenotype, our lab has observed a copper resistance at 0.17% on YPD plate and 0.14% in liquid cultures of S. cerevisiae lacking the mdm35 protein, when compared to the Wild Type strain. Our investigation measures the levels of expression in the cup1 and ctr1 promoters in an attempt to link this observation to a potential mechanism contributing to this resistance.

Sara Fauver, University of Utah Life Sciences We know that novel genetic variants have driven evolution for millions of years and that natural selection favors phenotypes most suited for survival, leading to the enormous diversity of life we see today. However, what remains unclear, are the patterns of mutations that lead to large phenotypic changes. For example, do mutations in a single gene of large effect lead to morphological changes more often than numerous mutations in genes of smaller effect? Also, do these mutations occur more often in protein coding regions or regulatory regions of DNA? Finally, are the same genes or gene pathways used repeatedly across lineages when parallel phenotypes evolve?

Daniel Hall, Brigham Young University Life Sciences OBJECTIVE(S): Predictive analytics (PA) is increasingly being used in the delivery of healthcare. Whether PA can improve patient handoffs on a busy surgical service is unknown. This study aims to determine if predictive models for acute cellular rejection (ACR) episodes and biliary complications after orthotropic liver transplantation (OLT) can be built in order to improve patient care.

Spencer Alexander, Dixie State University Life Sciences Antibiotic resistance has increased with each new developed medication, creating new problems as bacteria become more difficult to defeat. Some of these bacteria are resistant because they can excrete an extracellular polymeric substance known as a biofilm. The polysaccharide-based biofilm matrix allows the colony to communicate, absorb nutrients, and exchange genetic material giving it an advantage in possible resistance through plasmid exchange. At present, there are no effective antimicrobial agents that can safely treat and prevent resistant bacteria like ORSA. Biofilms have a negative impact ranging from human pathogenesis down to economic expenses. In order to break down established biofilms, we utilized newly developed organic salts known as ionic liquids. These novel liquids have been observed to prevent bacterial colonies and biofilm formation, possibly by introducing intermolecular interactions that disrupt the chemical bonding in biofilms. The morphology of the microbes was characterized and observed to determine the effect of the ionic liquids on biofilms. Inhibition studies were also performed to determine antimicrobial efficiency of the ionic liquids as a function of organic structures. These novel ionic liquids provide an unprecedented, effective and efficient method to combat resistant bacteria, which could have tremendous impacts in achieving sterile environments in medical and remote settings.

Mark Soffe, Utah State University Life Sciences SSBs are DNA binding proteins that are essential components of cells and play key roles in DNA replication, repair, and recombination. Here we utilize two biochemical properties associated with the E. coli SSB protein to develop a novel procedure to purify proteins using a resin-free strategy. 1. E. coli SSB binds to single stranded DNA (ssDNA) with extremely high affinity (K = 1013 M-1), indicating very tight binding. 2. It is also a unique protein with respect to its purification – it is possible to obtain greater than 95% pure SSB from the total cell lysate without using any sort of column or resin, utilizing polyethyleneimine (PEI) and ammonium sulfate precipitation. Our design uses SSB as an affinity/solubility tag to enhance the solubility and expression of difficult-to-purify proteins, and allows for the simple, resin-free purification using PEI and ammonium sulfate precipitation. There also may be a possibility to co-express protein dimers and possibly tetramers using this method. Constructs have been made that include the SSB gene, along with the ability to fuse any gene of interest, as well as a TEV Protease cleavage sequence allowing for proteolytic cleavage after gene expression. Two genes of interest have been cloned in thus far—TEV protease and Rad51. In this proposal I outline experiments to develop this strategy further and test our proof of principle concept and its application to a broader set of target proteins.

Marilize Van der Walt, Utah State University Life Sciences Urbanization is accompanied by a lot of changes to the landscape that have the potential to affect the native species inhabiting the area. If animals are chronically exposed to these anthropogenic disturbances and are unable to acclimatize, changes in circulating glucocorticoid hormones may cause adverse effects to the animal’s health, such as an impairment in innate immune activity. One such disturbance is human-induced noise. Using the side-blotched lizard, Uta stansburiana, because of their localized habitat and inability to escape such disturbances within the urban environment, we looked at the field and lab components of noise as a stressor. We first measured decibel levels in urban and rural field sites in their natural habitats in St. George, Utah, and conducted a lab study exposing U. stansburiana to either a synthesized urban sound recording or no sound recording (control) for nine days. We collected blood samples and measured circulating corticosterone and testosterone concentrations and bactericidal ability to determine if there are endocrine and immune alterations in response to increased noise decibels. Our results show that lizards exposed to urban noise experienced increased corticosterone levels indicative of stress. Furthermore, bactericidal ability was indirectly affected by noise through significant correlations between body condition and corticosterone and testosterone concentrations. These results indicate that an increase in ambient decibel levels acts as a stressor to animals in urban areas. By dissecting out an individual component of a complex stressor we can better understand the effects of urbanization as a whole.

Molly Van Engelenhoven, Utah State University Life Sciences For thousands of years farmers have known that soil organisms can destroy crops but only in the past ten years have researchers begun to understand that complex interactions among soil organisms and plants can determine plant diversity and productivity. Much of the research in this young field of study has come from qualitative greenhouse experiments and separately from simple mathematical models. Here, we report findings from a six-year plant-soil feedback (PSF) field experiment that assessed the effects of soil organisms on the growth of twelve plant species common to the intermountain west. Initially, twelve native and exotic species were randomly assigned to 1550 experimental plots in a field. After four years of growth, the plants were killed. Next, the same plant species were randomly assigned to the 1550 plots again to test how each plant species responded to the soil microbial communities ‘cultivated’ by each other plant species. Two years later plant growth responses to different soil types were measured. Most plants showed large growth differences on different soil types. For example, Bromus tectorum grew twice as much on Pseudoroegneria spicata-cultivated soils than on self-cultivated soils. When the plant growth responses observed in this experiment were used in a mathematical model, PSFs were found to play a critical role in determining which plants were likely to be dominant in a community and how productive these different plant communities would be. In summary, this large-scale experiment provides strong evidence for the role of PSFs in plant productivity and diversity. Results have implications for natural methods of effective native plant restoration, biofuel production and weed control.

Michael Morgan, Utah State University Life Sciences The advancement of biologically derived alternatives to petroleum diesel fuel requires a multifaceted approach. At Utah State University we use an interdisciplinary team including the Colleges of Engineering, Agriculture & Applied Sciences, and Science in conjunction with industry partners to drive innovation in improving the science behind petroleum diesel alternatives. With increasing petroleum use, depleting reserves, increasing emissions standards, and other factors, there is need for petroleum diesel alternatives that are cost effective, offer improvement, and perform similarly to petroleum diesel. Our team has focused on the use of oleaginous microbes utilizing low value effluent and waste sources including sugars and CO2 to create biofuels. We have focused on a yeast, Cryptococcus curvatus, and a microalgae, Nannochloropsis salina which have shown high yields of fuel per cell mass. Using these microbes we have utilized USU’s own direct trans-esterification reaction to create sufficient quantities of biodiesel for engine performance and emissions testing, including a subset of ASTM tests characterizing the fuels from each organism. Our initial engine testing used petroleum diesel as a baseline in conjunction with commercial soybean biodiesel to establish the quality of our microbially derived biodiesel. Testing in stationary diesel engines and on the Bonneville Salt Flats has proven our microbial fuels perform similarly to soybean biodiesel and comparably to petroleum diesel. To further improve biological diesel replacements we have begun working to create green diesel, hydrocarbons from a biological source, using a novel method of hydrothermal liquefaction. Preliminary results of those tests are presented here. Through a multifaceted and interdisciplinary approach USU is successfully improving petroleum diesel alternatives from microbial sources including characterization of the properties of these fuels and is working to create the fuels at the scale necessary for exhaustive engine performance and emissions testing including ASTM testing of all important fuel properties.

Michaelle Cadet, Utah Valley University Life Sciences Utah County, Utah has an estimated population of 540,000 residents and is considered to be a non-attainment area for criteria pollutants such as PM-10 and CO. High levels of these contaminants may increase the risk of respiratory diseases. Additional environmental issues exist including water contamination and eutrophication of Utah Lake. These environmental issues are frequently on the news and warnings are issued by the Department of Environmental Quality notifying citizens of the potential health concerns associated with environmental pollution. With these announcements, it is expected that Utahans are acutely aware of environmental issues, particularly, in the academic settings. The purpose of this study was to investigate the status of environmental awareness among students at Utah Valley University and to correlate the results to socio-demographic categories. Utah Valley University, located in Utah County, is a public institution of higher education with approximately 30,000 registered students. Data for this study was collected through a survey using a multistage sampling technique with population stratified based on colleges and schools within the university. Sample size included approximately 1,000 students. The survey contained 12 environmental questions relating to recent local, national and international media exposure. Standards and protocol of the International Research Board were employed. We hypothesize that married, educated, religious males will positively correlate with higher levels of environmental awareness, but will be less than atheists. Additionally, those identifying with the Republican Party will have lower levels of awareness. No significant difference will be found between majors. Furthermore the young, poor and ethnic populations will be less aware than their more affluent counterparts. Results of this study will be used to inform residents of environmental issues and the associated health concerns. Additionally, this study will be used to inform legislators about the importance of environmental education in the community.

Jeremy Rehm, Brigham Young University Life Science The recent surge of interest in personality differences between individuals of a single population or members of differing populations has generated numerous new hypotheses that may aid in elucidating patterns of ecology and evolution that were previously considered improbable. Two hypotheses relevant to fish biology relate the size of an organism from a certain predation environment to the level of boldness it exhibits. The first of these (predation hypothesis) predicts small individuals living with predators should not express boldness comparable to their larger counterparts, whereas the other (metabolic hypothesis) predicts the exact opposite. Our study investigated these hypotheses using two sister-taxa fish species in Panama (Brachyrhaphis roseni and B. terrabensis) that exhibit two size classes (large and small) and live in differing predation environments. Additionally, because males are smaller than females in both species, we could look at size-boldness relations within each species. The study, as in others, defined boldness as the amount of time for an individual to emerge from a shelter and into an unfamiliar territory. When the species are analyzed collectively, our results support previous findings that fish from high-predation environments tend to be bolder than those without predators; males tend to be bolder than females; and both mass and standard length positively correlate with boldness. However, within species analyses find that mass and standard length have no significant relation to boldness, and gender was only significant in the predation-exposed B.roseni, where males were bolder. These interesting findings contrast with previous studies, and lead us to question the value of these size-related hypotheses in the process of speciation and, ultimately, evolution.

Ethel Tackie-Yarboi, Westminster College Life Sciences The brine shrimp, Artemia franciscana are an important source of nutrients for many of the over 15 million migratory birds that visit Great Salt Lake (GSL) in Utah each year. As well as being a source of food, the brine shrimp are an intermediate host to cestode parasites that infect many of the bird species. Although the effects of cestode infection in brine shrimp and the contribution of brine shrimp to cestode circulation in birds have been studied, little research has yet addressed the rate of cestode infection or molecular phylogeny of these cestodes. We collected brine shrimp from three sites in GSL and tested individuals for cestode infection using previously identified and newly designed cestode specific PCR primers that amplify the 18S ribosomal RNA gene. Our preliminary results showed that the Spiral Jetty site from the North arm of GSL had a 2% infection rate, the Antelope Island site from the South arm had a 45% infection rate, and the shrimp collected at the Black Rock site, also in the South arm, had a 32% infection rate using our cestode specific PCR assays. We also have preliminary DNA sequencing results that demonstrate that we are amplifying cestode DNA, which is most closely related to members of the genus Hymenolepis. Our data suggest that there may be a higher prevalence of cestode infection in the shrimp in the South arm than those from the North arm of the Great Salt Lake. Our goal is to extend our study in order to better identify the species of cestodes that infect brine shrimp using further DNA sequencing and to expand our infection rate samples to better estimate the percentage of the brine shrimp population that is infected by the cestode parasites, as well as test samples from several years.

J David Symons, University of Utah Life Sciences Cardiovascular complications (e.g., arterial dysfunction) are more prevalent in patients with type 2 diabetes (T2DM). Patients with T2DM have elevated levels of circulating free fatty acids (FFAs). We have shown that when bovine aortic endothelial cells (BAECs) are treated with the physiologically relevant FFA palmitate, protein phosphatase 2A (PP2A) activity increases, phosphorylated endothelial nitric oxide (NO) synthase (eNOS) to total eNOS (p-eNOS:eNOS) decreases, and metabolites of NO production decrease. NO is an important endothelial-derived relaxing factor that is vasculoprotective. As such, FFA-induced, PP2A-mediated reductions in p-eNOS:eNOS and NO production might explain why vascular complications are more common in pathologies associated with lipotoxicity e.g., T2DM and diet-induced obesity. Recently we showed in BAECs that if PP2A is inhibited using okadaic acid (OA), palmitate-induced increases in PP2A activity, and reductions in p-eNOS:eNOS and indices of NO production are negated. We sought to translate these finding from BAECs to the intact organism. However, OA cannot be used in vivo. Lixte Biotechnology 1 (LB1) is a PP2A inhibitor that has been used in vivo in the context of cancer research. The purpose of this study was to determine the efficacy of LB1 under our experimental conditions, with the long-range goal of using LB1 in mice. Further, we used this opportunity to optimize the measurement of NO directly using electron paramagnetic resonance spectroscopy (EPR). BAECs were treated for 3 h with vehicle (V), 500 µM palmitate (P), 4 µM LB1, or P + LB1 (n=10 per treatment). P increased (p<0.05) PP2A activity (50±12%), and decreased (p<0.05) p-tyr307:PP2A (29±9%; redundant indicator of increased PP2A activity), p-eNOS:eNOS (30±3%), and NO production (27±9%). All P-induced effects were prevented by concurrent treatment with LB1. Future experiments will determine whether chronic treatment of mice with LB1 is capable of suppressing PP2A activity in intact arteries.