Skip to main content
Utah's Foremost Platform for Undergraduate Research Presentation

2014 Abstracts

Differentiating Molecular Subtypes of Breast Cancer Using High-Frequency Ultrasound

January 01, 2014 12:00 AM
Caitlin Carter, Utah Valley University Life Sciences High-frequency (HF) ultrasound (20-80 MHz) has been previously used to detect differences in microstructures and cell materials of different breast tissues types. These differences were used to distinguish between benign and malignant pathologies in different breast tissues. This same technology is predicted to be able to improve methods of detecting changes in cellular activity before changes in pathology take place. The purpose of this study was to use HF ultrasound to detect changes in the actin cytoskeleton, extracellular matrix (ECM), and integrin signaling, therefore differentiating the molecular subtypes associated with these changes in cell biomechanical properties. It is predicted that these cellular changes will also be associated with changes in the ultrasonic properties of breast cancer cells. The ability to rapidly and inexpensively detect the genetic changes or molecular subtypes of breast cancer would greatly impact and personalize patient treatment as well as provide more precise surgical removal of malignant and premalignant tissue. In order to test this hypothesis, four different breast cancer molecular subtypes including luminal A, luminal B, Her2+, and basal (triple negative) were grown as monolayer cell cultures. These subtypes were chosen because of their range of aggressiveness (luminal A as least aggressive and basal as most). After growth at different seeding levels, cell cultures were tested with a HF ultrasound system using a 50 MHz, 6.35-mm diameter immersion transducer and pulse-echo transmission. The data was compared to simulations using multipole expansions which predicted ultrasonic scattering based on possible variations in the biomechanical properties of malignant cells. The analyzed data showed differences in the spectra of waveform signals between each tested breast cancer molecular subtype. It is anticipated that this technique would provide an efficient and cost-effective method for differentiating between different molecular subtypes of breast cancer.

Edge effect on vegetation type and cover along a popular recreational trail

January 01, 2014 12:00 AM
Cynthiann Heckelsmiller, Weber State University Life Sciences Heavy foot traffic compacts soil, leading to decreased gas exchange, water permeability, and reduced nutrient cycling. Plant communities are defined by the availability of nutrients, water, and other resources.

Protein phosphatase 2A activation contributes to endothelial dysfunction that occurs in mice with diet-induced obesity

January 01, 2014 12:00 AM
Xin Wan, University of Utah Life Sciences Endothelial dysfunction exists in individuals with diet-induced obesity (DIO) and type 2 diabetes (T2DM). Markers of endothelial dysfunction include reduced phosphorylation (p) of endothelial nitric oxide (NO) synthase (eNOS) to total eNOS (p-eNOS:eNOS), and attenuated endothelium-dependent vasorelaxation. Free fatty acids (FFAs) are elevated in individuals with DIO and T2DM. Our laboratory has shown that when: (i) endothelial cells are incubated with saturated FFA palmitate; (ii) mice are infused with lard-oil; and/or (iii) when mice are fed with high-fat diet, protein phosphatase 2A (PP2A) binds directly with eNOS. When this occurs, the association among Akt-Hsp90-eNOS is disrupted, p-eNOS:eNOS is impaired, and endothelium-dependent dysfunction occurs. This is prevented using pharmacological and genetic approaches that limit production of FFA metabolite ceramide. It is unknown whether PP2A inhibition per se is protective. We hypothesized that arterial dysfunction in obese vs. lean mice is prevented by PP2A inhibition. Seven-week-old, male, C57B16 mice consumed standard (CON, n=20) or high-fat (HF, n=20) chow for 12-weeks. Subgroups (n=10) of CON and HF mice received IP injections of saline (vehicle; V) or Lixte Biotechnology 100 (LB1, 1 mg/kg/day) for the last 14-days. Preliminary experiments verified that LB1-treatment for 3 and 21 days decreases (p<0.05) arterial PP2A activity. HF mice gained weight and developed peripheral glucose intolerance vs. CON mice regardless of LB1 treatment. Endothelium-dependent vasorelaxation was impaired (p<0.05) in HF-V vs. CON-V mice, but dysfunction was less severe (p<0.05) in HF-LB1 mice. p-eNOS:eNOS was reduced (p<0.05) in arteries from HF-V vs. CON-V mice, but p-eNOS:eNOS was similar in arteries from HF-LB1 and CON-LB1 mice. Akt and Hsp90 co-immunoprecipitation with eNOS was impaired (p<0.05) in HF-V vs. HF-CON mice, but this was not observed in arteries from HF-LB1 and CON-LB1 mice. These findings suggest that PP2A activity suppression in vivo is sufficient to preserve endothelial function in obese mice.

Three-Dimensional Modeling of Facial Nerve VII

January 01, 2014 12:00 AM
Dani Peterson, Brigham Young University Life Sciences Due to its long and complicated trajectory through the cranium, facial nerve VII (CN VII) can be damaged in surgeries, sometimes resulting in facial muscle paralysis. Surgical removal of acoustic neuromas and parotid tumors, in addition to surgical repair of the temporomandibular joint disorder are associated with a risk of damage to CN VII. In addition, insertion of auditory implants can damage the nerve, as can improper stimulation to the nerve after the implantation has occurred. We will create a three-dimensional (3D) model based off of data from dissection of the nerve in a human cadaver in order to give physicians a greater in vivo knowledge of the pathway of CN VII. We have dissected the lateral side of the right half of the head to the level of the parotid gland, identified the parotid plexus of CN VII, and followed its five branches. In addition, we are currently following the nerve through the internal auditory meatus on its pathway through the temporal bone. In preparation for the modeling MicroScribe technique described below, we have imaged the head using Magnetic Resonance Imaging (MRI) at BYU. These images will be used as a template for the nerve reconstruction model. After completing the dissection, we will track the nerve trajectory using a MicroScribe 3D Digitizer. The MicroScribe technique is used to create 3D computer models of any physical object. The user sets reference points and uses the stylus to trace data points of the object’s contours. Our final product will be a 3D spatial computer mapping of CNVII, as well as a mapping of the skull, parotid gland, and other landmarks to put the nerve model into context. We hypothesize that with our approach and MicroScribe technique, we will be successful in creating an accurate model of CN VII in the head.

Biological effect of of oils spill dispersant corexit

January 01, 2014 12:00 AM
April Moorer, Weber State University Life Sciences Corexit is a dispersant used in the gulf of Mexico as a reactive measure to counteract the oil spill of April of 2010. Studies reveal that toxicity is produced and has impacted marine life. Research shows that reproductivity is diminished as a consequence. Paralysis, tumor development, and fatalities are also proven to occur. The purpose of this experiment is to study effects on the physiological structure of brine shrimp at various life stages resulting from the exposure to toxicity induced by corexit.

Rapid adaptation of d2 dopamine receptor responses following acute ethanol

January 01, 2014 12:00 AM
Joseph Linzey, Brigham Young University Life Sciences Dopamine (DA) D2 receptor expression parallels DA levels in the brain and these autoreceptors on DA neurons been shown to be modulated by long-term ethanol exposure. We have previously demonstrated that VTA GABA neurons also express D2 receptors, and that DA and D2 receptor agonists markedly enhance the excitability of VTA GABA neurons, opposite to their well-known inhibition of DA neurons. Most importantly, D2 receptor antagonists block ethanol inhibition of VTA GABA neurons and D2 receptor expression in VTA GABA neurons down-regulates with chronic ethanol. This study evaluates short-term D2 receptor adaptation in VTA GABA neurons and in DA release in the nucleus accumbens (NAc) by acute ethanol. In electrophysiology studies in anesthetized rats, periodic iontophoretic application of DA, or the D2 agonist quinpirole, markedly enhanced VTA GABA neuron firing rate, which was initially inhibited by ethanol, but resulted in latent and marked rebound excitation 30-60 min following injection. Using fast scan cyclic voltammetry (FSCV), we evoked DA signals in the core of the NAc by electrical stimulation of the medial forebrain bundle at the level of the lateral hypothalamus (60 Hz, 24 pulses). Intraperitoneal (IP) administration of ethanol (1.0-3.0 g/kg) dose-dependently decreased the amplitude of the MFB-evoked NAc DA signal. IP administration of the D2 antagonist eticlopride (1 mg/kg) markedly increased (250%) the amplitude of the evoked DA signal. When ethanol was administered after eticlopride it increased the amplitude of the DA signal an additional 42%. These findings suggest that ethanol induced decreases in evoked DA release may be due to autoreceptor feedback. Work is in progress to evaluate the short-term expression of D2 receptors in VTA GABA neurons following acute ethanol and to evaluate the effects of ethanol-induced short and long-term adaptations in VTA GABA neuron D2 expression in mediating ethanol effects on DA release in the NAc.

Olfactory Processing in the Brain: The Impact of Lateral Glomerular Interactions on Odor Representation

January 01, 2014 12:00 AM
Kyle Hansen, University of Utah Life Sciences Our brain processes physical stimuli from the world around us to shape how we perceive our environment. In the olfactory system, this processing begins with olfactory sensory neurons that relay information about distinct odorants to a region of the brain called the olfactory bulb. Olfactory sensory neurons express the same odorant receptors converge onto structures within the olfactory bulb called glomeruli. Here, we map lateral interactions between glomeruli to better understand how olfactory information from the environment is processed within the olfactory bulb. Using GCaMP6, a fluorescent calcium indicator, in awake mice we can observe optogenetically-evoked patterns of lateral connectivity between glomeruli in the olfactory bulb. The findings from this study will help clarify potential interglomerular connection schemes and provide understanding of how local circuitry in the olfactory bulb transforms the representations of sensory information before it is relayed to higher brain areas.

Improved Understanding of Non-Pathogenic Biophysical States in a Class of Neurodegenerative-Related Proteins in Hopes of Establishing a Baseline for Protein-Misfolding Disease Characterization

January 01, 2014 12:00 AM
Joe Passman, University of Utah Life Sciences Neurodegenerative disorders and amyloidosis are thought to be a consequence of the misfolding of intrinsically disordered proteins (IDPs). In non-pathogenic states, IDPs are thought to manipulate their configurational ensembles (CEs) – through partially folding and/or forming secondary structure – to create binding sites for the multiple interaction partners necessary for cell-signaling, recognition, and regulation. An obstacle to accurate in vivo understanding of non-pathogenic mechanisms is that little is known about the impact of the crowded cellular environment on the global (i.e. partial folding) and local structural characteristics (i.e. secondary structure) of IDPs. It is difficult to understand why an IDP may misfold if little atomistic structural understanding exists regarding IDP functional mechanisms in non-adherent physiological states.

Pas Kinase Activation by Snf1

January 01, 2014 12:00 AM
Brady Evans, Brigham Young University Life Sciences Nutrient-sensing kinases, such as AMPK and mTOR, play a key role in regulating cellular metabolism. They recognize nutrient levels within the cell and distribute nutrients accordingly. The failure to coordinate metabolic processes can lead to diseases such as diabetes, obesity and cancer. PAS kinase is another member of the nutrient-sensing kinase group that regulates glucose distribution in the cell. Despite its clear importance, little is known about the mechanisms regarding PAS kinase activation and its function. Studies have shown that mice without the PAS kinase gene are resistant to weight gain and maintain insulin sensitivity when placed on a high fat diet. Weight gain and insulin resistance are associated with increased rates of diabetes, cancer and other metabolic diseases. AMPK is currently the key target for the treatment of diabetes and has been found to be a cancer target as well. The yeast homologue, Snf1, is necessary for activation of yeast PAS kinase. Further studies have shown that PAS kinase is activated under the same respiratory conditions as Snf1. The aims of this project is to determine if Snf1 directly phosphorylates PAS kinase and to determine the effects of this phosphorylation. We have found that PAS kinase is activated quickly when cells are placed under Snf1-activating conditions. In addition, Snf1 is necessary and sufficient for this activation. Snf1 also copurifies with PAS kinase suggesting this activation is direct. PAS kinase purified from wild type verses Snf1-deficient cells shows phosphorylation on two key residues, S1020 and S1035. In addition, Snf1 directly phosphorylates PAS kinase in vitro. Together these findings suggest that Snf1 directly phosphorylates PAS kinase in order to regulate cellular glucose homeostasis

Discovering Dermapteran Relations: Phylogeny of earwigs based on molecular evidence

January 01, 2014 12:00 AM
Michael Naegle, Brigham Young University Life Sciences Dermaptera is a comparatively small order of insects with approximately 1800 species placed in three suborders. While the majority of earwig species are placed within the suborder Forficulina and are free-living with forceps-like appendages, two dermapteran lineages have a very unusual morphologies and life histories. The viviparous Hemimerina live epizoically on giant rats in tropical Africa where they feed on fungi growing on the rats’ skin. Hemimerina lack eyes and wings and the cerci are filiform. The viviparous Arixenina are associated with bats in Malaysia and the Phillippines, and they feed on bat skin gland secretions. They have reduced eyes, are wingless, and possess straight cerci. The phylogenetic position of the suborders Arixenina and Hemimerina relative to Forficulina have previously been unclear; however preliminary analysis suggest the phylogenetic position of the suborders Arixenina and Hemimerina are nested within Forficulina, with ectoparasitism evolving multiple times within this order. We generated DNA sequence data from three nuclear (18S, 28S and H3) and two mitochondrial (COI and TUBA) genes for representatives of all three suborders and outgroups. A phylogeny was reconstructed to address the following questions: (1) Does Hemimerina + Arixenina form a monophyletic group and support a single origin of parasitism or are there multiple origins of parasitism? (2) Is Forficulina monophyletic with respect to these parasitic lineages? (3) Are morphological similarities shared by the ectoparasitic forms synapomorphic or homoplasious characters?

Seasonal Water Availability and Temperature Drive Subalpine Carbon Soil Flux

January 01, 2014 12:00 AM
Josh Harvey, Brigham Young University Life Sciences Soil CO2 flux represents an important pathway of carbon transfer from ecosystems to the atmosphere. Soil CO2 flux can be altered by global warming-driven changes in seasonal temperature and water availability. Subalpine ecosystems have high levels of carbon in their soils that are stabilized by low temperatures and low microbial activity during long and snowy winter seasons. Subalpine ecosystems can be important sinks for carbon, storing carbon that otherwise would be in the atmosphere contributing to global warming. In our study we show how changes in temperature and water availability during springtime increase the levels of subalpine carbon output. So long as the carbon outputs outweigh carbon inputs, increases in soil flux would amplify global warming. The amplification of global warming would loop back to affect soil fluxes again (by raising temperatures, melting snow earlier, and changing precipitation patterns) thus creating a positive feedback system. Understanding what feedbacks are present in a climate system and their underlying mechanisms will improve our forecasts of changes in atmosphere chemistry and temperature.

Predicting species distribution of Agave utahensis through environmental niche modeling

January 01, 2014 12:00 AM
Austin Pearce, Brigham Young University Life Sciences Agave utahensis acts as a keystone species across its native range in the Mojave Desert and Colorado Plateau (Gentry, 1982). As a keystone species, Utah agave contributes to soil formation along barren mountain ridges, and has provided starch-rich sustenance to Native American tribes. Furthermore, taxonomists consider each of the two subspecies, kaibabensis and utahensis, to have considerable morphological variation (Gentry, 1982) within their own unique ecological niches. Given the importance of Utah agave, the high degree of variation, and its unique ecological niches, there is surprisingly little information published regarding its physiological ecology. In fact, no effort has been made to determine the population densities of Utah agave due to the remoteness of the region and its difficult terrain (e.g., the Grand Canyon). Therefore, geospatial analysis tools specific to environmental niche modeling provide a powerful means through which these issues and knowledge gaps can be effectively addressed. My goal is to develop a species distribution model by joining known locations of Utah agave with climatic and environmental data in MaxEnt and ArcGIS software. Such a model can be used by others for further ecological field studies of Utah agave and its subspecies. Additionally, the approach I employ can be used as a pattern for mapping distributions of other important plant species in remote and difficult-to-access regions of the world.

Exploring new means of transdermal delivery of nutrients

January 01, 2014 12:00 AM
Brittany Hammontree, Dixie State University Life Sciences Depending on metabolic conditions or dietary preferences, people can often become deficient in critical vitamins and minerals. For example, a number of people are deciding to become vegetarians, and vitamin B12 deficiencies could become a huge epidemic, as this essential vitamin is only obtained through meat products. This issue was the driving force to look deeper into new ionic liquid materials and how they could be used as a transport agent for vitamin B12, along with other vitamins. Ionic liquids are organic salts that are currently being explored in many scientific fields due to their unique properties. However, using ionic liquids as a transporter in transdermal applications has yet to be explored Developing new mechanisms of administering nutrients via transdermal processes can increase the bioavailability and effectiveness of vitamins and minerals that often cannot survive oral administering due to the acidity and molecular absorption via the stomach. This research focused on finding the right ionic liquid with high solubility of the individual vitamins. Several ionic liquids were developed, and the different vitamins were tested for solubility levels. This greater solubility allows for maximum exposure of the vitamin during transdermal delivery. In particular, two different vitamins were tested – vitamin K and Protoporphyrin, a chemical analog to vitamin B12. Additionally, the effect of these ionic liquids on the physiology of the blood and plasma as it enters the body past the skin layers is critical to understand. In addition to transdermal applications of vitamins, transport of these vitamins to other countries and remote locations could have tremendous implications. Ionic liquids tend to increase shelf life of solutes, and the availability to provide these materials during medical missions or service trips would be increased substantially, particularly in more remote settings.

Predation Shapes Personalities in Brachyraphus Fishes

January 01, 2014 12:00 AM
David Money, Brigham Young University Life Sciences Interest in animal personalities, and particularly the effect that different environments have on personality, has increased dramatically over the past decade. Understanding how individuals vary in their behavior, and if there are consistent differences among populations from divergent selective environments, lays the foundation for studies focusing on the contribution of divergent behavior in species formation. To date, studies that have focused on how personalities differ across ontogenetic stages have failed to compare populations that occur in dramatically different environments. Our study attempts to fill this void by studying the ontogeny of personality in populations that have evolved in environments with different levels of risk (i.e., predation vs. no-predation). We tested the expression of different personality traits evolution across ontogeny (i.e., from juveniles to full grown adults) in two sister species of live-bearing tropical fish, Brachyraphis roseni and B. terrabensis. These species have evolved in different selective environments, with B. roseni having evolved in an environment where predators were present, while B. terrabensis evolved in an environment lacking predators. We assessed the boldness expression of individuals from several groups in populations, namely juveniles, small adults, and large adults. To measure boldness, we used an emergence test, and also an exploration and activity test (i.e., ratio of movement to idleness during an allotted time period). Our study provides evidence for an important relationship between predation environment and the evolution of personality traits across ontogeny.

The Role of Endocannabinoid Receptor GPR55 on Learning and Memory

January 01, 2014 12:00 AM
Bradley Prince, Brigham Young University Life Sciences Learning and memory occur due to adaptive brain changes in response to our environment. These changes are mediated by synaptic plasticity, particularly within the hippocampus, where spatial and declarative memories occur. Plasticity can either strengthen or weaken synapses, known as long-term potentiation (LTP) or long-term depression respectively. While many forms of synaptic plasticity are N-methyl-D-Aspartate receptor-dependent, recently endocannabinoids were identified to mediate several new forms of hippocampal synaptic plasticity. Endocannabinoids bind to receptors such as cannabinoid receptor 1 (CB1) and transient receptor potential vanilloid 1 (TRPV1), and mediate several forms of plasticity, including in the hippocampus. However, new research has demonstrated a non-CB1/TRPV1-dependent endocannabinoid synaptic plasticity in the hippocampus. While the receptor(s) involved is currently unknown, several potential candidate receptors that bind the endocannabinoid anandamide have been identified. These are orphan G-protein coupled receptors (GPRs) whose distribution in the brain and/or function is unknown. GPR55 is of particular interest as it activates second message systems, including increasing intracellular calcium. Using quantitative RT-PCR, electrophysiological and memory behavioral tasks we examined hippocampal GPR55 expression and function. GPR55 is indeed expressed in hippocampus of both rats and mice. Cellular expression is currently being examined and appears to be rare in interneurons and more likely expressed by pyramidal cells. Interestingly, application of the GPR55 agonist LPI (2 µM) to wild-type mice demonstrates a decrease of LTD in brain slices. This LPI effect was not noted in GPR55 knock-out mice in the presence of LPI. This data suggest GPR55 is physiologically relevant in the hippocampus. This is the first direct evidence we are aware of that a novel endocannabinoid receptor directly effects hippocampal LTD. Because neurodegeneration that affects memory is typically associated with an increase in LTD, this provides a potential target to slow the advance of diseases such as Alzheimer’s.

Use of Phage and Vancomycin Treatments Against Staphylococcus aureus Biofilms

January 01, 2014 12:00 AM
Kelly Hoerger, University of Utah Physical Science Implant devices such as orthopedic, dental, and cochlear implants are commonly utilized as part of many medical treatments. However, these foreign objects are susceptible to bacterial contamination, thereby putting the host at risk of an infection that is challenging to eliminate due to biofilm formation. Biofilms are formed when a bacterial cell adheres to and colonizes such metal or plastic surfaces. The cells aggregate to form and embed themselves in a thick and protective polysaccharide matrix, making biofilms resistant to many antibiotic treatments.

Geoelectric and Magnetic Characteristics of Fracture Traces: A Tool for Groundwater Exploration in Igneous Rocks

January 01, 2014 12:00 AM
Jeremiah Rundall, Utah Valley University Physical Sciences Utah Valley University has a long-term program of groundwater development in the Trans-Mexican Volcanic Belt in the state of Guanajuato, Mexico, in cooperation with Choice Humanitarian. Thus far, we have drilled a successful 50-m well in basalt and an unsuccessful 22-m well in rhyolite. Well sites have been chosen based on spring locations and fracture traces (linear features as seen on aerial photos). The objective of this study is to measure and model the geoelectric and magnetic characteristics of fracture traces as a means of determining which fracture traces are most indicative of actual fractured rock. Thus far, studies have been carried out on 20 fracture traces in rhyolite in the Trans-Mexican Volcanic Belt, six fracture traces in rhyolite/diorite/monzonite of the La Sal Intrusives in the La Sal Mountains of Utah, and one fracture trace in rhyolite of the Soldiers Pass Formation west of Utah Lake. The total magnetic field has been measured at about 1-m spacing along profiles perpendicular to fracture traces using the Geometrics G-856 Proton Precession Magnetometer. Geoelectric soundings for resistivity, chargeability and self-potential have been carried out both on and off fracture traces using the Iris Instruments Syscal Junior Resistivity Meter with the Schlumberger array parallel to the fracture trace at spacings in the range AB/2 = 2.29-137 m. Geoelectric profiles have been carried out perpendicular to fracture traces (array parallel to the fracture trace) at spacings AB/2 = 15.2 m and 137 m. Geoelectric and magnetic modeling is being carried out using the 1X2D-GM Magnetic Interpretation Software and the 1X1D Sounding Inversion Software. Preliminary results indicate that some fracture traces are associated with both magnetic and electrical resistivity lows consistent with intermediate depth (~ 50 m) fractured rock, although not all fracture traces have these characteristics. Further results will be reported at the meeting.

Optimizing the measurement of monomethylmercury in natural waters by direct ethylation

January 01, 2014 12:00 AM
Christopher Mansfield, Westminster College Physical Science The method currently used to measure monomethylmercury (MMHg) in natural waters involves a lengthy distillation step in order to remove certain ions and dissolved organic matter that have been reported to interfere with the subsequent reaction in which MMHg is ethylated. It has recently been reported that the analysis of MMHg in seawater by direct ethylation was successfully carried out, thus removing the day-long distillation. However, the effect of many potential interfering ions and compounds on this method have not been characterized, and it has not been tested for use in natural freshwaters or the hypersaline waters of the Great Salt Lake. Thus, these became the two goals of this project. We found that optimal ethylation conditions included buffering samples to pH 4.0-4.1, reducing the amount of sodium tetraethylborate, adding EDTA to complex interfering trace metals, and adding chloride to overcome interferences by thiols and organic matter. We have since employed this improved method to analyze MMHg in natural water samples from fresh and saline lakes, achieving > 90% MMHg spike recoveries without the use of distillation.

Band gap tunability of protein-based ferrhihydrite nanoparticles

January 01, 2014 12:00 AM
Stephen Erickson, Brigham Young University Physical Sciences “The spherical protein ferritin has often been used to fabricate nanoparticles of various shapes and compositions with its walls. Ferritin occurs naturally with a ferrihydrite (FeOOH) mineral core, but it has also been used to synthesize nanoparticles of several other semiconductors. While the methods for creating these nanoparticles within ferritin are well established, the characterization of such nanoparticles is not. Previous studies on native ferrihydrite core ferritin disagree on the band gap, giving values anywhere from 1.1-3.5 eV, depending on the method. We have used absorption spectroscopy to measure these band gaps with an unprecedented accuracy of up to .01 eV. This method also allowed us to determine that ferrihydrite nanoparticles are indirect gap semiconductors. By employing this method on particles of various sizes, we have shown the effects of quantum confinement, resulting in variations in the band gap. We also provided the first ever direct evidence that ferritin works to crystalize its core with time, an effect that has long been theorized but never observed. By characterizing the effect of size and time on nanoparticle band gap, we have shown the potential for selectively tuning that gap. This opens up a world of possible applications in light harvesting and photo detectors. By controlling the band gap, we will be able to select which wavelengths of light are absorbed, allowing for full spectrum photovoltaic cells and wavelength specific optical detectors. Future studies will focus on nanoparticles of other metal hydroxides and various anion replacements to further expand our tunable range of band gaps.

An insulating gasket for electrical measurements in a diamond anvil cell

January 01, 2014 12:00 AM
Jasmine K Bishop, University of Utah Physical Sciences In my presentation, I will discuss the method of creating insulating gaskets for high pressure experiments using diamond anvil cells. In standard diamond anvil cell pressure experiments, metal gaskets are typically employed. However, in order to study the electric and magnetic properties of a sample without worrying about interference of the metal gasket itself, insulating gaskets are used. The insulating material needs to be ductile and yet hard enough to maintain a certain level of structure. Epoxy is ideal for ductility but is not hard enough to maintain a diamond indent under pressures generated by diamond anvil cells. When mixed with a diamond powder however, the mixture is both ductile and structurally sound. In this study we have modified the previous methods of insulating gasket preparation by addition of a metal sheet to increase the strength and ductility of the gasket. The part of the gasket that is metal can be used as an electrical lead to measure sample’s conductivity. In this design the mixture of diamond and epoxy is applied to a thin 100 micrometer metal sheet and a focused beam of high power IR laser is used to drill a hole in the gasket . I will describe the design of the optical path and the details of the gasket preparation and will present some of the gaskets that I have made.

Characterization of undocumented bonneville shorelines with evidence of possible tsunamis

January 01, 2014 12:00 AM
Brittney Thaxton, University of Utah Physical Sciences There are many undocumented shorelines seen in Utah. As many as 30+ shorelines have been identified along the edges of the basin throughout the state. The purpose of this study is to characterize undocumented shorelines and identify potential evidence for tsunamis that might have occurred in Lake Bonneville thousands of years ago in areas such as Little Mountain, Stansbury Island, and Promontory Point, Utah. Lake Bonneville existed 32-10K years ago and was influenced by the Wasatch fault which was active as early as the Miocene. Scarps of this age are common and range between 15-20 feet in height (Machette, Personius, Nelson, Schwartz, Lund 1991; Dinter, Pechman, 2004a and 2004b). Several faults beneath Lake Bonneville could have produced tsunamis. The more water that is displaced the greater the tsunami will become and leave a greater impact onshore (Dutykh and Dias 2009). The East Great Salt Lake fault cuts NS across Bear River Bay east of Promontory Range. This fault line is an excellent candidate for causing a tsunami during the Lake Bonneville highstand because it is beneath the Great Salt Lake meaning it would have uplifted the entire water column of Lake Bonneville and since it is also close to the Promontory Range, it is likely it would leave tsunamite evidence along the shore. The fault rupture interval is between 3,000 and 3,500 years meaning fault ruptures could produce a tsunami during the lake’s high stand. Tsunamite is the term used for deposits related to tsunamis. The tsunamite features found along the shorelines will be similar to sedimentary features such as normally graded sand, mudstone clasts, and other gravel deposits that are out of place with the known shorelines (Shanmugam 2006). This is a unique opportunity to discover ancient tsunami evidence in Utah, a topic that has yet to be pursued.

Use of the Manning Equation to Estimate Stream Discharge through Natural Slot Canyons and Artificial Slots

January 01, 2014 12:00 AM
Jeff Selck, Utah Valley University Physical Sciences Stream discharge through narrow, deep slot canyons can be a major source of groundwater recharge in the arid Southwest. Various state and federal agencies use the empirical Manning Equation to predict the discharge through artificial slots created for diversion of rivers around coal mines. However, it is not obvious that the Manning Equation could be applied to slot canyons or artificial slots as the data base used for development of the Manning Equation did not include either natural streams or artificial structures for which most of the friction occurs along the sides of the channel. The objective of this research is to develop an empirical formula for estimating the Manning roughness coefficient for flow through narrow, deep slots. The objective is being addressed by measuring stream discharge through natural slot canyons in southern Utah that are fed by perennial streams, springs or dam outlets. Based on measurements at ten sites along eight streams, the best estimate for the Manning roughness coefficient is n = 0.873nJ exp(5.108A/w2) where A is stream cross-sectional area, w is stream width, and nJ = 0.39S0.38R-0.16 is the roughness coefficient estimated by Jarrett (1984) for high-gradient streams, in which S is slope of the stream bed and R is hydraulic radius (ft). The new formula estimates stream discharge with a mean accuracy of 44%. On the other hand, Jarrett’s (1984) formula underestimates stream discharge by 1-2 orders of magnitude for aspect ratios A/w2 in the range 0.6-0.7. The new formula will be refined by additional measurements on slot canyons, artificial diversions, and a laboratory hydraulics bench. It is hoped that the new formula will lead to a more realistic design for artificial slot diversions.

Structural health monitoring of natural arches in the Moab area

January 01, 2014 12:00 AM
Ben White, University of Utah Physical Sciences We evaluated the ambient noise wavefield of select arches in the Moab area as a means to assess changes in their structural health over time. Our measurements revealed that the fundamental frequency of vibration at Mesa Arch and Corona Arch are both in the range of 3 Hz, while other spectral peaks are likely related to higher-order vibrational modes. We use numerical modeling for modal analysis in an attempt to predict and visualize the various modes of vibration and their frequency Resonant frequencies are projected to change with snow or rain loading or after a strong earthquake. By monitoring resonant frequencies over time and under various environmental conditions, our goal is to detect reversible and/or irreversible changes that may accompany damage of these arches.

Wavelength Detection from Filtered Photodiodes

January 01, 2014 12:00 AM
Nils Otterstrom, Brigham Young University Physical Sciences Filtered photodiodes show potential as inexpensive laser wavelength meter. Photocurrents are measured digitally. The photocurrent is digitized using externally controlled integration times to achieve the highest precision possible from the digital to analog converters on the photosensor chip. Using an algorithm we’ve developed and calibrated intensity curves, we can precisely calculate wavelength from the output of the different photodiodes. Limitations due to etaloning from reflections off of the surfaces of the filters were analyzed and effectively mitigated, allowing the device to achieve high precision with a stability of 0.102 nm over several hours.

How many class ii wells present a risk for induced seismicity?

January 01, 2014 12:00 AM
Isaac Allred, Utah State University Physical Sciences We examine the number and location of Class II wells in the central U.S. to constrain future work on the potential for induced seismicity. The EPA, state oil & gas commissions, scientific papers, and media stories frequently state that there are ~140-160 k Class II wells. Excluding California, we expected to find approximately 120 k wells; but instead found ~ 82 k active injectors in the available databases. State datasets vary in accessibility, availability, and content of well data. Lack of digitized well data also limited our online search, and several states require FOIA requests to be filed. State databases with poor searching and sorting functions further complicated data mining, requiring a well-by-well search, and for several states, well locations and injections were difficult to determine. Common discrepancies between EPA well totals and state database totals appear to be due to counting of plugged and abandoned wells, and wells that are permitted but not in use. No data has been retrieved for about 1,600 wells on tribal lands and Indian Country, and several states would not provide “confidential” well data. Of the active injectors, at least 55 k wells inject into producing, pressure-depleting oil and gas formations and are less likely to generate damaging earthquakes. Of the ~ 16 k non-EOR wells, we found 3,400 wells that inject at depths > 1.8 km, where most M > 3.0 midcontinent earthquakes occur. We will present examples of data from several states, that show the locations and depths of injectors, earthquakes, depth to basement, and we will provide an overview of the public file sharing system of the data. We will search for correlations between the depth of injection, the number of injection wells, recent seismic activity, the nature of the subsurface geology, and regional stresses.

Pitocin Use and Incidences of Neurodevelopmental Disorders

January 01, 2014 12:00 AM
Derek Oliver, Dixie State University Social and Behavioral Sciences The use of birth interventions has been steadily increasing over the past several decades based on largely non-medical factors. It is thought that the various birth interventions (i.e. forceps, vacuum extraction, epidural, etc.), pitocin use, the mother’s attachment and psychological constructs during pregnancy are related to the incidences of neurodevelopmental disorders. Thus, the current study attempts to understand the association between birth intervention and the development of neurodevelopmental disorders. Three hundred mothers of children between one and five years of age were recruited using social media, flyers and phone calls. Mothers provided access to their medical records of the birth of their child as well as incidences of neurodevelopmental disorders in their children. Mother’s hospital records were obtained to determine length of pregnancy, duration of labor and rate and duration of pitocin use. It is expected that there will be a significant correlation between developmental disorders and interventions broadly. Further, it is expected that pitocin use will be positively correlated with incidences of autism.

Characterizing water-related land use differences across urban river reaches

January 01, 2014 12:00 AM
Dusty Pilkington, Weber State University Social and Behavioral Sciences Boundaries dividing political authority rarely match natural water flow. Multiple agencies, often with competing policies and agendas, manage water within single watersheds. This discrepancy can render management efforts ineffective. Local water agencies include soil conservation districts, local health departments, and engineering agencies. Agencies have varying funding sources, managerial structures, and jurisdictional boundaries. Fragmentation can frustrate management efforts at watershed scales. Comparisons between natural watersheds and human political geography can therefore be helpful in avoiding conflicts. This research examines discrepancies between political and physical geographies for sections of three Utah rivers : the Logan and Provo Rivers, and Red Butte Creek. These rivers are being targeted for intensive research as part of a statewide, multidisciplinary water sustainability project, titled iUTAH. Water-related land use (WRLU) data acquired from Utah’s Automated Geographic Resource Center were analyzed using ArcGIS geoproceessing tools. WRLU contributing to water quality in urban river reaches is described. WRLU was examined using three boundary sets, comparing the political geography of the river reaches, as depicted in municipal boundaries and management areas of community water providers, to their physical geography, as depicted in United States Geologic Survey Hydrologic Unit Code (HUC) watersheds. WRLU mixes using political infrastructure were contrasted with land use mixes from HUC boundaries. WRLU was classified in seven categories: residential, commercial/industrial, riparian/water features, irrigated agriculture, non-irrigated agriculture, farmsteads, and parks/open spaces. A gradient from rural Heber, to urbanizing Logan, to fully urbanized Red Butte Creek is shown. While Red Butte Creek HUC boundaries showed 53.8 % residential land use, Red Butte Creek municipal boundaries and community providers totaled 26 % and 39 % residential use, respectively. Differences emerged when irrigated agriculture was assessed using HUC boundaries. Irrigated agriculture totaled 0.2 % in Red Butte Creek, with Logan showing 29. 7% and Heber irrigated agriculture sitting at 41.1 %.

Health Literacy and Child Language Brokers: How Bilingual Children and Spanish- Speaking Parents Navigate the Medical Setting

January 01, 2014 12:00 AM
Luz Maria Carreno, Utah State University Social and Behavioral Sciences Significance: Patients’ health literacy, or ability to comprehend and understand health information, influences their health status, knowledge about medical care and conditions, and hospitalization and adherence rate (Andrus, & Roth, 2002). Low-English proficiency patients are especially at risk, given they must overcome English and health literacy barriers.

Parental Distress in Mothers of Very Low Birth Weight Infants: Examining the Influence of Medical,Family and Maternal Mental Health Factors.

January 01, 2014 12:00 AM
Laurin Wilson, Utah State University Social and Behavioral Sciences Premature birth and subsequent hospitalization of an infant in the Newborn (or Neonatal) Intensive Care Unit (NICU) can be an extremely stressful time for parents and has been associated with maternal depression, anxiety, and decreased coping ability (Hack, Taylor, Klein, & Mercuri-Minich, 2000; Hughes, McCollum, & Sheftel, 1994; Partridge et al., 2005; Shaw, Sweester, St. John, Lilo, Corcoran, Jo, & Horwitz, 2013). Interventions to reduce parents’ stress levels during the hospitalization of their very preterm infants have mixed results (Boyce, et al., 2008; Matricardi, S., Agostino, R., Fedeli, C., & Montirosso, R., 2013). This study examined the medical, family, and maternal mental health factors that influenced feelings of parental distress for mother of very premature infants.

Maternal Directives as Predictors of Defiance Aggression in 2 Year Olds.

January 01, 2014 12:00 AM
Mitchell Reid, Utah State University Social and Behavioral Sciences Chronic childhood aggression can be the beginning of a developmental trajectory that begins with minor aggression, moves on to physical fighting, and then to violence (Loeber & Farrington, 2000). Behaviors during toddlerhood may be more malleable than later in childhood (Reid, 1994). To better understand what predicts children’s early aggressive behavior we examined the role of parenting behaviors, parent mental health, and child characteristics in a sample of toddlers. Sixty-five mothers and their toddlers between the ages of 17 and 24 months (60% female) participated in the study. Mother-child dyads were videotaped during teaching and clean-up tasks. Mothers also responded to several questionnaires to assess toddlers’ social-emotional behaviors, language development, attachment security, and temperament and their own parenting stress and depression. The teaching and clean-up tasks were coded for maternal language supporting behaviors such as asking questions and providing praise, directives, expansions, and labeling. We examined the correlations between the independent maternal (depression, parenting stress, and language supporting behaviors) and child (age, gender, language development, temperament, and attachment security) variables and the dependent variable of aggression/defiance. Correlations between attachment security (r = -.28, p =.03) and maternal directives during the teaching task (r = .33, p =.01) and during the clean-up task (r = .39, p =.00) showed statistical significance. We included these variables in a regression model and found that attachment security and maternal directives during the teaching and clean-up tasks accounted for 29% of the variance in maternal reports of toddlers’ aggression and defiance. These results suggest that toddlers with greater attachment security and with mothers who use fewer directives in everyday tasks are rated as less aggressive and defiant than those with less attachment security and with mothers who use more directives. The full regression model and early intervention implications will be presented.

Religiosity and Attitudes Towards Cosmetic Surgery among Latter-day Saint Women

January 01, 2014 12:00 AM
Danielle Jennings, Utah Valley University Social and Behavioral Sciences The popularity of surgical cosmetic procedures continues to climb in the U.S. (ASPS, 2012) and in other industrialized economies (ISAPS, 2011). This includes women in conservative cultures like Morocco and Afghanistan (Women of Afghanistan, 2012). This research was undertaken to examine attitudes towards plastic surgery among women who are members of the LDS Church. Utah boasts one of the highest concentrations of plastic surgeons in the U.S. (Lim, 2011) and is also majority LDS (Phillips and Cragun, 2011), rendering it ideal for this study. Data regarding attitudes towards surgery and levels of religious participation were collected from LDS women [n = 100] and men [n = 25] in Utah through a pilot survey administered in spring, 2013. The sample included women 19 — 81 years of age, 64% of whom were married, and 63% had grown up in Utah. Results indicated levels of religious participation were in keeping with other research involving Mormons (Hill et al., 2008) and 67% of women knew a female member of the Church who had had cosmetic surgery. Attitudes towards surgery varied by age and marital status. A second survey was created and administered via Qualtrics to LDS women [n =473] and men [n = 116] across the U.S. Results suggest variation in attitudes by geographic location, along with variation by gender, age, marital status, and familiarity with surgery. These results suggest aspects of Mormon life in Utah contribute to the popularity of surgery among Church members. These may include the pressures of dating and earlier ages at marriage (Maffly, 2010), coupled with demographic changes (Cragun and Phillips, 2011), as well as earlier ages at first birth of children (Davidson, 2008).

A Pilot Study of Social Support for Birth-Grandparents of Adopted Children

January 01, 2014 12:00 AM
Renette Goodrich, Utah Valley University Social and Behavioral Sciences In the realm of Adoption most of the focus is given to the birth parents and the adoptive couple. However, there are many more people involved, some being the birth grandparents, who are the parents of the expecting parents. These grandparents, most of who are dealing with feelings of loss and grief, have been left to struggle through the experience by themselves. A scholar from Well’s, were the law requires social services to be offered to the extended family, conducted studies with grandparents; some of whom received social services and some who did not. His found a statistically significant difference in those who participated in the social services and those who did not. Grandparents who took advantage of social services where less stressed and more excepting of the adoption. This pilot study was conducted at one agency in Utah. Because of HIPPA laws the only access was to those grandparents who attended the support group. This study also showed social service support for these grandparents where significant; by lowering the stress level of dealing with a child’s unexpected pregnancy and helping them with direction and acceptance. This was a qualitative and quantitative survey handed directly to the participants to fill out and return. The agency where this survey was conducted and other associated agencies are interested in these results to better improve currents services and reach out to other grandparents who are not currently participating in these groups. This topic has not been studied in the United States; thus this study will add to the body of scholarly knowledge on this topic.

Becoming an Adult on an Island

January 01, 2014 12:00 AM
Peter Busche, Brigham Young University Social and Behavioral Sciences An undergraduate study on young people in the Channel Islands (near England and France. Such questions will be addressed, such as: What makes growing up in a tiny Island community unique? How does leaving the Island for the outside world affect the young peoples’ transition into adulthood? This project consists of a descriptive outline based on 2 months of ethnographic research, as part of a 10,000 word senior thesis. Current anthropological and psychological theories are part of the overall construction of the data.

The Role of Empathy in Attachment

January 01, 2014 12:00 AM
Michelle Hammon, Dixie State University Social and Behavioral Sciences A caregiver’s ability to meet their infant’s physical and emotional needs is important to development. Infants who are unable to get their needs met are at risk of developing an insecure attachment with their caregiver. The ability of the caregiver to empathize with and appropriately respond to the needs of their infant is dependent on the caregiver’s attachment representation. An online survey collected data from 112 college students. Participants completed the Experiences in Close Relationships-Revised (ECR-R) scale and the Interpersonal Reactivity Index (IRI). Insecure attachment representations were associated with the ability to empathize. Anxious-ambivalent attachment was positively associated with concern and distress and avoidant attachment were negatively associated with distress. These results suggest that attachment representations are important for the development of empathy.

Sustainability Officers: Centralized Leadership in Sustainability within Higher Education Institutions and Municipalities

January 01, 2014 12:00 AM
William Ng, Utah Valley University Social and Behavioral Sciences This paper draws on leadership and behavioral theory in a public context to ascertain the full necessity of the sustainability officer to engender, sustain, and develop sustainability movements in higher education institutions and municipalities. As sustainability rises in awareness and demand from university and municipal stakeholders — influenced by political and educational discourse, the sustainability officer plays a vital role in conceptualizing, interpreting, and manifesting the abstract principles that stems from sustainability movements. Specifically, this study looks at the emergence of the sustainability officer, who they are, where they come from, what they do, and the outlook of future trends. Moreover, this paper looks at the structural positions of where sustainability officers fit in the organizational hierarchy and question whether they fall in line with contemporary political theory of public organizations. Furthermore, through interviews with Sustainability Managers/Coordinators/Directors, an intimate look into where they have been effective, where they could progress, and what they envision for themselves in the future has been garnished. Finally, these interviews manifest the establishment of the pragmatic connection between organizational theory and practice, allowing an exposition of the evolution of centralized sustainability leadership.

Measuring nonconformity to familial values

January 01, 2014 12:00 AM
Chantelle Fitting, Brigham Young University Social and Behavioral Sciences Rebelliousness is often the product of an effort to express individualism and is an outlet for uniqueness (Imhoff & Erb, 2009); however, it can have very detrimental consequences. When a person strives to not conform to his or her family’s beliefs and values, familial relationships become strained, and the potential arises for the family to be torn apart. This nonconformity manifests itself through the beliefs a person has and the actions they take that are markedly different from those chosen by family members. The Nonconformist Assessment Scale (NCAS) is a ten question survey that is used to measure an individual’s inclination to rebel against his or her family unit. With the NCAS, we seek to discover indications of early rebelliousness, which are manifested through a person’s deviation from the family in areas such as weekly activities and personal interests. By detecting the seeds of nonconformity that arise in early life within the family, family therapists can intervene before more severe rebelliousness such as illegal activity, abandonment of core family values, or other dangerous behaviors emerge. Cronbach’s Alpha of the NCAS was found to be .7195, giving the test acceptable reliability. The Pearson bivariate correlation indicated that every item except item nine correlated positively with at least seven other items. The items loaded onto a single factor, and the variance explained by this factor was .7019. Thus the NCAS is a good indicator of nonconformity to familial values. However, the domains of actions and beliefs were not discernible as the items loaded only onto a single factor, so further research could be conducted that includes items where these domains are more easily separated from each other.

Negative Pressures and Adolescent Admission Experience in Outdoor Behavioral Treatment

January 01, 2014 12:00 AM
Clinton Hardy, University of Utah Social and Behavioral Sciences Coercive treatment practices, while controversial, are commonly employed (by mental health professionals and parents) when admitting adolescents into outdoor behavioral healthcare (OBH; e.g., outdoor/wilderness therapy) and residential-based treatment settings. While coercive practices have been investigated in various adult treatment contexts, to my knowledge no study has examined coercive practices or pressures among adolescent treatments. As the first of its kind, my report investigates the relationship between adolescent-perceived coercive treatment pressures (i.e., negative pressures) and their affective reactions reported when entering OBH treatment. Negative pressures, or the perceptions of “threat” and “force” during admissions, are measured via the Negative Pressures Subscale (NPS) of the MacArthur Admission Experience Survey (AES). Affective reactions to entering treatment are measured via the Positive and Negative Affect Schedule – Expanded Form (PANAS-X). Although this study is still taking place, my interim analysis (N = 36, age range: 14-17) has so far demonstrated the following predicted relationships between negative pressures and affective reactions in OBH treatment admissions: An adolescent’s increased perception of negative pressures significantly correlates with (a) increased affective distress (r = .377, p = .037), (b) decreased affective positivity (r = -.61, p < .001), and (c) increased affective hostility (r = .464, p = .003). Therefore, significant medium to large correlational effect sizes are reasoned to be imminent. In recognizing that negative pressures might not only relate to an aversive admissions experience, but also negatively impact or delay positive treatment outcomes, future research is concluded to be essential.

Evolution of Burrowing Mayflies: Tusks be gone

January 01, 2014 12:00 AM
Stephanie Bartlett, Utah Valley University Physical Sciences Ephemeroptera, commonly referred to as mayflies, are found throughout the world. Within the order of Ephemeroptera resides a superfamily, Ephemeridea, commonly called burrowing mayflies. This common name was acquired due to certain physical and behavior characteristics present as nymphs. Ephemeridea nymphs live in the silt of aquatic environments and have adaptations for burrowing which include strong legs, as well as mandibular tusks. One exception to this is the family Behningiidae, which burrow, but lack the commonly associated mandibular tusks. Morphological data supported Behningiidae as sister to the other tusked burrowing mayflies, indicating that first burrowing behavior evolved and was later followed by the development of tusks(McCafferty; 1975 and 2004). While morphological data provides important insights into the evolution and phylogeny of mayflies, the development of molecular phylogenetics offers new contributions when determining evolutionary relationships within this superfamily. Objective: The purpose of this study is to investigate the relationships of the families of burrowing mayflies in order to test the hypothesis of tusk evolution. Methods: The specimens were acquired from collection efforts and colleagues. For each specimen the following laboratory procedures were carried out: DNA extraction, gene amplification via polymerase chain reaction, visualization via gel electrophoresis, and DNA sequencing. The genes targeted for sequencing included 12S mitochondrial rDNA, 16S mitochondrial rDNA, 18S nuclear rDNA, 28S nuclear rDNA, H3 nuclear protein coding, and CO1 mitochondrial protein coding. Data was also acquired from Genbank in order to augment missing data. Taxon sampling consisted of around 20 ingroup and 5outgroup taxa. Phylogenetic relationships were estimated using Maximum Parsimony, Maximum Likelihood, and Baysian methods. Conclusion: The families Behningiidae, Potamanthidae, Palingeniidae were supported as monophyletic. Behningiidae nested well within the other burrowing families. Hence, tusks evolved and were subsequently lost in the family Behningiidae even though it retained the burrowing lifestyle in the nymph.

Nondestructive Isolation of Forensic Analytes with Ionic Liquids

January 01, 2014 12:00 AM
Mattie Jones, Dixie State University Physical Sciences New methods aimed at forensic analysis of sensitive, minute samples are critical to the intelligence community. In particular, successful extraction of dyes from materials found at crime scenes will provide innumerable benefits for matching, identifying, and finding origins of these materials and dyes. Current methods of isolating components of samples by their unique chemical properties are lengthy and often destroy important forensic evidence. Ionic liquids possess the necessary chemical properties to ensure efficient extractions, while maintaining the forensic signatures of the original materials. They also provide a one-pot approach that avoids intermediate species and increases analyte yield while extracting and separating constituents in a more efficient manner. By combining traceable dyes with an ionic liquid, the versatility of single-component extraction-separation-identification was demonstrated. Analysis using absorption and fluorescence spectroscopy validated complete extraction and recovery of trace analytes. Following extraction and isolation, identification by infrared spectroscopy has provided evidence of preserved quality and complete separation of material and dye. This novel approach to forensic analysis is advantageous particularly when sample sizes are extremely limited, but it can be readily scaled to larger applications. Developing a simple and affordable method of achieving specific molecular interactions provides a solution for often unidentifiable evidence in crimes. Harnessing the versatility of ionic liquids in a high-yielding recovery and efficient single-pot methods will enhance forensic abilities for the intelligence community and forensic investigators.

Improving a Laser Color Sensor Based on a Photodiode Array Using Measurements Made with a 461nm Tunable Laser

January 01, 2014 12:00 AM
Tyler Jones, Brigham Young University Physical Science This project takes advantage of an inexpensive integrated circuit to create a wavelength meter. The circuit has an array of photodiodes, each of which has an optical filter of a different color. The filters cause each diode to respond differently to different wavelengths of light. The relative amplitudes of the signals are then used to compute a wavelength based on known responses for various wavelengths recorded in a calibration stage. The project focuses on improving precision of the hardware and developing an algorithm to do the computation.

Novel Bioactive Compounds Produced by Juniperus osteoperma

January 01, 2014 12:00 AM
Kip Brower, Utah Valley University Physical Sciences Juniperus osteoperma has been reported to exhibit beneficial pharmacological activity against symptoms of diabetes, as a traditional treatment for kidney ailment and the essential oil of juniper berries has also been subject to investigation for antioxidant activities. Endophytes, particularly endophytic fungi, have produced numerous novel bioactive compounds, several of which are currently used as antifungal, anti-bacterial, and anti-cancer agents in organisms other than their plant hosts. This investigation looks into the possibility of endophytic fungi being a means of producing compounds that may be a functioning agent in antioxidant, antifungal, or hyper/hypoglycemic activities of Juniperus osteoperma. The investigation involves samples of Juniperus osteoperma collected from multiple different locations within Utah, and isolates the resident endophytic fungi.

Effects of Environmental Pollutants on Endophytes in Rumex crispus

January 01, 2014 12:00 AM
Yonic Michaca, Utah Valley University Physical Sciences It is well known that some of the worst air pollution in the country each winter is found along the Wasatch front in northern Utah. This study examines the effects of environmental pollutants on the production of the novel compounds produced by the endophytes found in Rumex crispus. The Rumex crispus plant was selected due to its natural medicinal uses. It is anticipated that environmental pollutants have an effect on the production of bioactive compounds in order to protect their plant host from foreign pathogens. The theory is that the more stressful environment a plant lives in, i.e. desert climates, high altitude, and man-induced stresses such as pollution, the more bioactive compounds the endophytes produce as a response to protect their plant host. This study analyzes the effects of environmental pollutants along the Wasatch front on the production of novel bioactive compounds produced by the endophytes found in Rumex crispus. Plant samples are also collected from sites near the Wasatch front, but they are not exposed to the same amount of air pollution to be used as a control.

Protein phosphatase (PP) 1 and PP2B do not contribute to palmitate-induced disruption of eNOS enzyme function.

January 01, 2014 12:00 AM
Ting Ruan, University of Utah Physical Sciences Cardiovascular complications are more prevalent in patients with diet-induced obesity and type 2 diabetes. Both of these conditions are associated with elevated levels of free fatty acids (FFAs). Elevated FFAs might precipitate cardiovascular complications by disrupting endothelial nitric oxide (NO) synthase (eNOS) enzyme function. The physiologically abundant saturated FFA palmitate decreases eNOS phosphorylation at serine 1177 (p-eNOS S1177) in a ceramide and protein-phosphatase 2A (PP2A) -dependent manner. p-eNOS S1177 is a positive regulatory site on the eNOS enzyme. As such, p-eNOS S1177 to total eNOS can be used as an estimate of eNOS enzyme function. We sought to determine the extent to which two other phosphatases that are abundant in the cytosol i.e., protein phosphatase 1 (PP1) and protein phosphatase 2B (PP2B) might contribute to palmitate-induced reductions in p-eNOS S1177 to total eNOS. Bovine aortic endothelial cells (BAECs) were treated for 3 hours with 500 uM palmitate or vehicle in the absence and presence of the PP1 inhibitor tautomycin (3 uM). p-eNOS S1177 to total eNOS was assessed using immunoblotting procedures. Palmitate-induced reductions (30±3%, p<0.05, n=3) in p-eNOS to total eNOS were similar in the absence and presence of tautomycin. These data indicate that PP1 does not contribute to palmitate-induced disruption of eNOS enzyme function. Next, BAECs were treated for 3 hours with 500 uM palmitate or vehicle in the absence and presence of the PP2B inhibitor cyclosporine (100nM). Palmitate-induced reductions (31±4%, p<0.05, n=3) in p-eNOS to total eNOS were similar in the absence and presence of cyclosporine. Taken together, these data suggest that neither PP1 nor PP2B contribute to palmitate-induced reductions in p-eNOS S1177 to total eNOS.

Controlled Zinc Oxide Hexagonal Prism Formation with Ethanol Amines

January 01, 2014 12:00 AM
Brielle Woolsey, Brigham Young University Physical Sciences A method of forming zinc oxide (ZnO) single crystal hexagonal prisms is derived from a standard sol-gel method. The new synthesis requires water, zinc acetate, and diethanol amine (DEA) to create a zinc hydroxide/zinc hydroxide acetate gel, which forms single crystal hexagonal prisms upon heating. Characterization of the gel was done by XRD as well as by XRD high temperature chamber (HTK) to determine the role of temperature in prism formation. SEM images showed hexagonal prisms were of uniform size (approx. 0.5 — 2 µm). TEM and electron diffraction images showed a change from randomly oriented particles to an ordered single crystal after heating. Ethanol amines, heating of the gel, and the zinc acetate salt proved to be critical to prism formation.

Utilizing Low-cost NIR Imaging Systems in Medical Physics

January 01, 2014 12:00 AM
Kim Nielsen, Utah Valley University Physical Sciences Infrared Thermography (IRT) has become popular in the medical field for various applications and procedures. One such application involves the use of IRT in the active detection of skin lesions and neoplasms. More specifically, medical professionals are using IRT as a non-invasive method for the early detection of harmful lesions, such as melanoma.

Bio-Inspired Molecular Manganese-Calcium Catalysts for Water Oxidation

January 01, 2014 12:00 AM
Nicholas Labrum, Utah State University Physical Sciences Increasing concerns on the anthropogenic climate change, rising global energy demands, and diminishing fossil fuels have urged the search of alternative carbon-neutral and sustainable energy resources, among which solar energy stands out as the most promising target since it is the largest exploitable resource. However, its nature of diurnal variation, intermittence, and unequal distribution requires efficient and cost-effective capture, conversion, and storage. Generation of chemical fuels, such as hydrogen, from solar energy input represents an appealing approach to meet this goal. An ideal scheme would tap hydrogen from the splitting of water with concomitant evolution of oxygen. Due to the nature of the four-proton and four-electron process, water oxidation is the bottle neck of the overall water splitting process. Nature catalyzes water oxidation using an oxygen evolving complex (OEC) in photosystem II. This project aims at mimicking the OEC to prepare and investigate bimetallic Mn-Ca catalysts for water oxidation catalysis. Calcium has been reported to be critical in water oxidation by OEC, however its functional role has not been well studied. By positioning a calcium atom in the second coordination sphere of manganese in molecular scaffolds, we are able to systematically study the functional role of calcium at the molecular level. Our project will prompt the development of water oxidation catalysis and benefit artificial photosynthesis at large.

Lyman-alpha Emission as a Probe of Galaxy Environments

January 01, 2014 12:00 AM
Joshua Wallace, University of Utah Physical Sciences We study the effect of the circum-galactic gas environment on the observed Lyman-alpha emission from Lyman-alpha emitting galaxies. These galaxies are primarily high-redshift star-forming galaxies that are important in understanding both galaxy and universe evolution. The Lyman-alpha photons emitted from these galaxies should hold valuable clues about the general environmental properties (such as gas velocity, density, and distribution) around galaxies, since the photons can be strongly affected and scattered by the neutral hydrogen atoms that make up the majority of the gas. However, explaining exactly how a galaxy’s environment affects its Lyman-alpha emission is very complex and currently cannot be predicted with complete certainty — every time a Lyman-alpha photon interacts with a hydrogen atom, its direction and frequency are changed, which in turn affects how far it will travel before the next interaction. We study the environmental effects on Lyman-alpha emission properties by applying Monte Carlo Lyman-alpha radiative transfer modeling to simple analytic models and find an important role of the anisotropic distribution of gas in determining the observed photometric and spectral properties of Lyman-alpha emission. We further perform a detailed investigation by applying the radiative transfer modeling to realistic star-forming galaxies in high-resolution cosmological galaxy formation simulation. From our analysis so far, we find the Lyman-alpha emission from the models shows a strong dependence on viewing angle, as well as correlations between observed spectral features and environmental conditions. We plan to model and analyze a large sample of simulated galaxies to better describe and statistically quantify the above dependence and correlations. Our study will lead to a better understanding of the effects galaxy environment on the observed Lyman-alpha emission and in turn provide a theoretical guide on how to use observed Lyman-alpha emission to learn about the environments of star-forming galaxies and hence galaxy formation and evolution.

Patterning and Functionalizing Carbon Nanotube Forests for Antigen Detection

January 01, 2014 12:00 AM
Benjamin Pound, Utah State University Physical Sciences Carbon Nanotube (CNT) Forests are vertically grown carbon nanotubes. They can be as tall as millimeters, with radii from less than one nanometer (single-walled) to tens of nanometers (multi-walled). Their high surface area to volume ratio provides a unique material system for biosensor applications. However, the CNT surface does not provide covalent bonding sites to many antibodies of interest. One approach is to attach linker molecules with aromatic rings via π-stacking to the CNT surface and activating the linker molecules to bind covalently to specific antibody molecules. Unfortunately, the conventional solution-based functionalization approach often leads to collapse of the CNT forest and hence a significant loss of binding sites. In this presentation we demonstrate that CNTs can be lithographically defined to form various structures that are resistant to liquid-induced collapse. We show that the CNT forest can be functionalized with 1,5-diaminonaphthalene as a linker molecule and its coverage can be characterized by fluorescence spectroscopy.

Metabolic Inhibition in Saccharomyces Cerevisiae

January 01, 2014 12:00 AM
Corey Wolf, Westminster College Life Sciences The yeast species Saccharomyces cerevisiae is used in numerous industries including brewing, baking, and winemaking. In recent years an increased demand for low ethanol beers has pushed breweries to develop a beer that is both rich in flavor and low in alcohol content. The current strategies employed to decrease the ethanol concentration in beer are costly, time intensive, and result in less flavorful beers. In this study, a method for decreasing alcohol content in the brewing process through the use of metabolic inhibitors of Alcohol Dehydrogenase is tested. Alcohol Dehydrogenase is the enzyme responsible for the conversion of acetaldehyde, an intermediate in the metabolic pathway of yeast, into ethanol. Two competitive inhibitors of Alcohol Dehydrogenase, Pyrazole and Fomepizole, were applied to small scale fermentations in varying concentrations in an attempt to decrease the overall ethanol yield of the beer. The application of either inhibitor resulted in an initial decreased rate of fermentation overall, measured as a change of sugar and ethanol concentration. In the later stages of fermentation, the rate of ethanol production returned to normal, suggesting the inhibitors were either catalyzed or removed by the cell. While this method for manipulating the products of fermentation did not yield the desired effects, this study did highlight the importance of the fermentation pathway for maintaing healthy Saccharomyces cerevisiae cultures. This research may be helpful in understanding the complete metabolic pathway of brewer’s yeast, with further application to organisms with conserved pathways.

Multimechanistic combination enhances selenium’s antiproliferative effect in prostate cancer cells

January 01, 2014 12:00 AM
Merrill Christensen, Brigham Young University Life Sciences Selenium (Se) has established chemopreventive efficacy against prostate cancer, the second leading cause of cancer death among men in the US. Currently, most studies only employ one single chemical form of Se, even though different forms of Se act through varied mechanisms to achieve their anticancer effects. In this study, we propose that systematically combining multiple forms of Se will produce an optimal combination of Se compounds to inhibit in vitro prostate cancer cell growth. As proof of principle, we (1) synthesized Se nanoparticles (nano Se), (2) determined the IC50s of methylseleninic acid (MSA), sodium selenite, and nano Se in PC-3 cells, (3) utilized mixture designs and response surface methodology to direct our combination experiments with these three compounds, (4) tested the fifteen combinations determined in the previous step, and (5) constructed a polynomial model to derive the optimum combination of MSA, sodium selenite, and nano Se to inhibit PC-3 cell growth. Our results showed that combining different forms of Se compounds enhanced its chemopreventive effect. Future studies will expand the use of three Se-containing compounds to four and demonstrate similar effects in xenograft mice.