2014 Abstracts

Lucas Lloyd, Utah Valley University Physical Sciences The high cost of air quality monitoring stations makes it difficult for citizens or local governments to monitor air quality in their own neighborhoods. For example, Utah County, Utah, with an area of 2141 mi2, has only four air-quality monitoring stations: (1) north Provo close to both Provo High School and Brigham Young University (2) Spanish Fork Airport (3) near State Street in Lindon (4) just south of SR-92 on 6000 W in Highland. The air-quality stations monitor levels of CO, NO2, O3, PM-2.5 (particulate matter smaller than 2.5 microns), and PM-10. The objective of this study is to find a much cheaper method of measuring air quality. The objective was addressed by measuring the magnetic susceptibilities of 10 replicates each of the leaves of 12 species of trees (cypress, crab apple, elm, flowering pear, green ash, honey locust, linden, Norway maple, pine, red maple, Russian olive, spruce) collected within a two-mile radius of each of the four air-quality monitoring stations in Utah County. After air-drying and crushing the samples, both low-frequency (0.46 kHz) and high-frequency (4.6 kHz) magnetic susceptibilities were measured with the Bartington MS3 Magnetic Susceptibility Meter. The best correlations between tree leaf magnetic susceptibilities and air-quality parameters were between the three-year average of PM-2.5 and the high-frequency magnetic susceptibility of leaves of pine (Pinus aristata) (R2 = 0.87) and Norway maple (Acer platanoides) (R2 = 0.86). The correlation was used with measured high-frequency magnetic susceptibilities of pine to estimate PM-2.5 in two unmonitored locations heavily impacted by highway traffic (corner of 800 N and I-15 and corner of University Parkway and I-15, both in Orem, Utah) on one day in August 2013. It was found that estimated levels of PM-2.5 were 9.5 µg/cm3 and 8.9 µg/cm3, respectively, which were within the EPA PM-2.5 Standard of 12.0 µg/cm3.

Benjamin Pound, Utah State University Physical Sciences Carbon Nanotube (CNT) Forests are vertically grown carbon nanotubes. They can be as tall as millimeters, with radii from less than one nanometer (single-walled) to tens of nanometers (multi-walled). Their high surface area to volume ratio provides a unique material system for biosensor applications. However, the CNT surface does not provide covalent bonding sites to many antibodies of interest. One approach is to attach linker molecules with aromatic rings via π-stacking to the CNT surface and activating the linker molecules to bind covalently to specific antibody molecules. Unfortunately, the conventional solution-based functionalization approach often leads to collapse of the CNT forest and hence a significant loss of binding sites. In this presentation we demonstrate that CNTs can be lithographically defined to form various structures that are resistant to liquid-induced collapse. We show that the CNT forest can be functionalized with 1,5-diaminonaphthalene as a linker molecule and its coverage can be characterized by fluorescence spectroscopy.

Buchanan Kerswell, Utah Valley University Physical Sciences This project is designed to investigate anthropogenic impacts on the geochemistry and physical characteristics of Utah Lake-Jordan River transition zone, in Utah Valley, Utah. The zone has experienced dramatic, multifaceted shifts since European settlement in 1847, especially in recent decades. Our chosen study location is uniquely situated to capture changes recorded in the sediment cores due to land use, nutrient enrichment, vegetation shifts and river dynamics since pre-settlement.

Nathaniel Wells, Utah Valley University Physical Sciences Previous research with bottle-shaped thermoacoustic prime movers has revealed hysteresis with transitions to higher modes as the cavity length is varied. A string with an abrupt change in mass density was studied to investigate potentially similar behavior. Three base guitar strings were studied at three different tensions with weights of 25, 30, and 35 lbs. Each string consisted of a “thin side” that was stripped to the stainless steel core and a “thick side” with an outer wrapping of nickel around the core. The strings studied had diameters of 0.65, 0.45, and 0.50 mm on the thin side and 2.14, 1.31, and 1.24 mm on the thick side, respectively. An anchor was attached on one end of a short board with a pulley at the other for hanging the weight. The end of the thick side of the string was attached to the anchor, and the string was guided over the pulley, with the change in mass density occurring approximately 12 cm from the pulley. Measurements were taken after placing a glass jar under the thick end of the string, between 42 cm and the position of the change in mass density, in 3-cm steps. The string was plucked and the dominant frequency was recorded with a microphone at each location. Frequency data is generally consistent with a solution to a 1D wave equation. Preliminary results indicate mode transitions occurring for all strings, with several hysteresis region candidates.

Lee Leavitt, University of Utah Life Sciences The ventral respiratory column (VRC) is a region in the brainstem shown to control breathing patterns in mammals. Using activation and inhibition of neurons in this region, classes have been assigned based on response-combinations. Using a mouse model, cells from this region are dissociated, plated and incubated with a dye that indicates changes in cytoplasmic calcium levels. Hundreds of cells are measured while varieties of pharmacological agents are applied. Response-combinations provide a profile of the receptors found on these neurons. Previously, varieties of cell classes were shown to contain NMDA receptors (receptors linked to learning and memory). However, specific compositions of subunits within these receptors are not known. These receptors are ligand gated ion channels composed of four non-covalently bound proteins. Each subunit has a different activation profile determined by interactions of agonist and antagonists. Conantokins (peptides isolated from snail venom) and other compounds further afford understanding of the architecture the assigned cell-classes. This project has continued to classify the subunit compositions of NMDA receptors with the ultimate goal of understanding which NMDA receptor subunits are present in each class. This will provide valuable information on the VRC’s function, and will allow for pharmacological innervations to change behavior in this region.

Dasom Kim, Brigham Young University Life Sciences The main purpose of the project is to compare the phylogeography of a species of stonefly (Klapopteryx kuschelli) and a species of dragonfly (Rhionaeshna variegata) in Patagonia. Specifically, the project will study how geography and behavior (i.e., their dispersal abilities) have affected their evolutionary histories. Of all the varied climates and geography in South America, comparatively few phylogeographic studies have been conducted with insects, especially in Patagonia. This study will provide an important foundation for a comparative phlyogeographic study of two insect groups inhabiting the same regions of Patagonia. Also, if funded, this project will give me an exclusive opportunity to interact with international research institutions in South America as well as their scientists.

Madison Landreth, Weber State University Life Sciences Enterococcus, a bacterial genus that normally inhabits the gastrointestinal tract of animals, can be pathogenic to humans, causing urinary tract infections, sepsis and other serious diseases. It is also one of the major causes of hospital acquired infections. One important complication of those infected with Enterococcus is the fact that these bacteria often have a high level of antibiotic resistance, making effective treatment of patients more difficult. While Enterococcus is a normal inhabitant of the gastrointestinal tract, it can survive outside its host in the environment, even in adverse conditions, such as the Great Salt Lake (GSL). In this experiment, hundreds of isolates of Enterococcus were collected from the Great Salt Lake, from various sites along the Weber River which flows into the GSL and from clinical sources. Isolates were tested for different phenotypic characteristics and for their resistant patterns against certain antibiotics. Preliminary results of the Kirby Bauer disk-diffusion assay demonstrated that 47% of enterococcal isolates from the Great Salt Lake were resistant to one or more of the five antibiotics compared to 98% of the clinical isolates. In contrast, in a previous study, as few as 15% of Enterococcus isolated from the fresh water sources were resistant to one or more of the five antibiotics. These data may have implications concerning the importance of anthropological impact on rates of antibiotic resistance in this genus.

Derrick Crawford, Brigham Young University Life Sciences Members of the FGF family of signaling factors are key components in distal outgrowth and patterning of the vertebrate limb. These factors are expressed and secreted by the apical ectodermal ridge (AER) on the distal margin of the limb. Blocking their function is known to truncate the limb skeleton. Conversely, replacing the AER with beads soaked in Fgf protein can rescue limb outgrowth and patterning. Our lab has demonstrated that one of the mechanisms whereby the Fgf/AER functions is to mediate directed outgrowth of the adjacent mesenchyme. As the AER regulates growth of mesenchyme toward itself, it would be predicted that the AER’s dimensions would be important for shaping the mesenchyme that it recruits. We have found that the shape of the AER changes over time in a manner that corresponds to the shape of limb elements as they form along the proximal distal axis. Further, mutants that exhibit defects in the dimensions of the AER show corresponding anomalies in the limb skeleton. Given these observations it would be predicted that an Fgf soaked bead being of fixed spherical dimensions would only be capable of forming a cylindrical, rod-shaped limb. A bead placed posteriorly fulfills this expectation whereas a bead placed apically does not. We provide a molecular explanation for this discrepancy. We have also manipulated the shape of the AER surgically and find that similar to beads the shape and the AP position of the AER dictates the shape of the forming limb skeleton.

Jake Gamboa, Brigham Young University Life Sciences It is estimated that approximately 350,000 people in the United Stated die annually from post-myocardial infarction arrhythmias. A majority of these people will undergo a surgery that results in partial or complete removal of the stellate ganglion and other nerve fibers of the pharyngeal space in an attempt to prevent over stimulation from the neurons to the area of dead heart tissue and, therefore, future arrhythmias. However, without a somatomototopy, it is unclear what physiological effects partial or full sympathectomies may have. We will create a three-dimensional map of the pharyngeal space nerve plexus which will, in turn, allow for a more accurate and precise surgery.

Teressa Paulsen, University of Utah Life Sciences Breast cancer is still the most common cancer among women regardless of race or ethnicity. The focus of our research is to uncover the mechanism breast cancer cells use to escape the inherent limitations of the telomere and obtain immortality. The protective end of a chromosome, the telomere, degrades with each cellular division. The cellular response to telomere dysfunction is to activate programmed cell death. Therefore, this type of damage normally limits the proliferative potential of the cell and subsequently carcinogenesis.

Josh Hall, Weber State University Life Sciences Reproduction in birds is extremely conservative with the vast majority of the birds adopting bird-egg contact incubation to maintain an appropriate microclimate for embryonic development (Deeming, 2004). The Great Salt Lake is a vital nesting site for American Avocets (Recurvirostra Americana) that shows extreme temperatures and hostile environments where nest success can be as low as 1 -14% (Cavitt, 2008). Constancy of incubation, i.e. the time that the eggs are in contact with an adult, is a major indicator of nest success and environmental conditions. Our goals were to examine some of the costs natural selection places on embryos and parents to maintain a constant embryo temperature. We hypothesized that incubation attentiveness would increase across the nesting cycle. Over 200 AMAV nests were surveyed. Thermal probes were used to record various nest microclimates at every minute. A pseudonest with painted chicken eggs was also created and a thermal probe was placed to measure the ambient temperature without any adult incubation. A motion sensitive camera was placed over nests to examine differences in parental care. Nests will be divided into three phases: early, mid, and late incubation. Thermal data will be analyzed using descriptive statistics and mean variance values to calculate how incubation constancy varied throughout these phases. We expect this data to tell us more on how natural selection is working on these populations and some possible theories of how this developed.

Goyeun Tun, University of Utah Life Sciences Chronic Fatigue Syndrome (CFS) and Fibromyalgia Syndrome (FMS) are disorders which their symptoms and treatments are not clearly known. CFS and FMS are not life threatening diseases; however, they can affect patients’ quality of life because they experience symptoms including exercise intolerance, need for bedrest, and debilitating chronic pain and fatigue with these disorders. The research from Dr. Light’s lab has shown that moderate exercise for 25 minutes causes changes in mRNA levels in CFS and FMS patients but not healthy controls. The objective of our study was to examine changes in white blood cell gene expression of CFS and FMS patients both on Lyrica and on placebo in a double-blinded, cross-over design (where each study subject was his or her own control) by using quantitative PCR gene expression analysis. The lab routinely analyzes blood samples for 48 different genes from study subjects and healthy controls collected before (baseline) and then 8, 24, 48 hours after exercise moderate exercise. My focus was on changes in expression of two ATP-responsive purinergic receptors, P2X7 and P2Y1, which have not been studied after exercise in CFS and FMS but have been associated with chronic inflammation and pain in animal models. White blood cell layers (buffy coat) were collected from samples, RNA was extracted and converted to cDNA. 384 well PCR plates were robotically loaded from 96 well source plates, then the PCR reaction was run in an ABI 7900 thermal cycler that tracks fluorescence in “real time” (real time qPCR). Analysis of results is in progress and will be reported on the poster.

Xin Wan, University of Utah Life Sciences Endothelial dysfunction exists in individuals with diet-induced obesity (DIO) and type 2 diabetes (T2DM). Markers of endothelial dysfunction include reduced phosphorylation (p) of endothelial nitric oxide (NO) synthase (eNOS) to total eNOS (p-eNOS:eNOS), and attenuated endothelium-dependent vasorelaxation. Free fatty acids (FFAs) are elevated in individuals with DIO and T2DM. Our laboratory has shown that when: (i) endothelial cells are incubated with saturated FFA palmitate; (ii) mice are infused with lard-oil; and/or (iii) when mice are fed with high-fat diet, protein phosphatase 2A (PP2A) binds directly with eNOS. When this occurs, the association among Akt-Hsp90-eNOS is disrupted, p-eNOS:eNOS is impaired, and endothelium-dependent dysfunction occurs. This is prevented using pharmacological and genetic approaches that limit production of FFA metabolite ceramide. It is unknown whether PP2A inhibition per se is protective. We hypothesized that arterial dysfunction in obese vs. lean mice is prevented by PP2A inhibition. Seven-week-old, male, C57B16 mice consumed standard (CON, n=20) or high-fat (HF, n=20) chow for 12-weeks. Subgroups (n=10) of CON and HF mice received IP injections of saline (vehicle; V) or Lixte Biotechnology 100 (LB1, 1 mg/kg/day) for the last 14-days. Preliminary experiments verified that LB1-treatment for 3 and 21 days decreases (p<0.05) arterial PP2A activity. HF mice gained weight and developed peripheral glucose intolerance vs. CON mice regardless of LB1 treatment. Endothelium-dependent vasorelaxation was impaired (p<0.05) in HF-V vs. CON-V mice, but dysfunction was less severe (p<0.05) in HF-LB1 mice. p-eNOS:eNOS was reduced (p<0.05) in arteries from HF-V vs. CON-V mice, but p-eNOS:eNOS was similar in arteries from HF-LB1 and CON-LB1 mice. Akt and Hsp90 co-immunoprecipitation with eNOS was impaired (p<0.05) in HF-V vs. HF-CON mice, but this was not observed in arteries from HF-LB1 and CON-LB1 mice. These findings suggest that PP2A activity suppression in vivo is sufficient to preserve endothelial function in obese mice.

Jacob Anderson, Utah Sate University Life Sciences Leafy spurge (LS) is an aggressive Eurasian forb that has been successfully reduced in many areas in western North America through the biological control releases of flea beetles. Long term studies of this phenomenon are sparse. Three flea beetle species were released in the mid-1990s at a site dominated by LS in Richmond, Utah. This study assessed the long term effects of LS biocontrol on an ecological community at this site by addressing five questions: (1) Is LS abundance significantly lower now than in the 1990s? (2) What plant species are replacing LS and are they native or non-native? (3) Have the flea beetle populations persisted since their initial release? (4) What part does soil type play in which flea beetle species now dominate at the site? (5) In response to their unexpected presence, what role may long-horned beetles contribute to the long-term reduction of LS? It was found that LS abundance has significantly decreased from the 1990s; the dominant plant species are those of non-native grasses; flea beetles have persisted in significantly smaller numbers, with Aphthona lacertosa being the most abundant; and long-horned beetles appear to play a significant role in the reduction of sexual success of LS. The results of this project have implications for land managers when considering the vegetative response to LS biological control and the importance of long-horned beetles for long-term in managed, LS-reduced habitats.

J David Symons, University of Utah Life Sciences Autophagy plays a central role in cellular quality control by destroying damaged or excess proteins, lipids, membranes, and organelles that accumulate in response to deviations from homeostasis. The existence and role of autophagy in endothelial cells (ECs) and blood vessels has not been established. Autophagy can be quantified by assessing the ratio of the membrane bound conjugate of microtubule-associated protein light chain 3 (LC3-II) to the cytosolic non-lipidated conjugate LC3-1 (LC3-II:LC3-I) or GAPDH (LC3-II:GAPDH) via immunoblotting. We sought to determine the extent to which a variety of cellular stressors induces autophagy in ECs and intact blood vessels. LC3-II:LC3-I or LC3-II:GAPDH was elevated (p<0.05) (i) 450±6% (n=4) in ECs incubated for 2 h in amino acid (AA)-deplete vs. AA-replete media; (ii) 47±3% (n=3) in arteries from fasted (14 h) vs. fasted / refed (1 h) mice; (iii) 40±2% (n=3) in arteries from mice that completed acute exercise vs. sedentary controls; (iv) 38±1% in arteries from exercise-trained vs. sedentary mice under basal conditions (n=2 per group); and was decreased (p<0.05) (v) 57±8% (n=4) in arteries from ~30 month-old (i.e., old) vs. ~6 month-old (i.e., young) mice. Further, indices of autophagy were elevated (p<0.05) 101±6% in ECs exposed to 3 h x 500 uM palmitate vs. vehicle (n=4), and 50±6% in arteries from obese vs. lean mice (n=4 per group). Thus, autophagy is altered in ECs and blood vessels in response to physiological (e.g., fasting, acute exercise, exercise training, aging) and pathophysiological (acute lipotoxicity, diet-induced obesity) stimuli. Ongoing research will determine the functional role of vascular autophagy in health and disease.

Janweb Lagazo, Brigham Young University Life Sciences The scientific community and the general public have long been interested in the effects of water pollution. Most studies on water pollution have focused solely on industrial pollution, but have failed to consider the potential impact of pharmaceuticals that unintentionally accumulate in aquatic ecosystems via wastewater treatment effluents. The purpose of this study is to advance our understanding on how these wastewater effluents affect aquatic ecosystems in Utah. We quantified the concentration of select pharmaceuticals in Hobble Creek using mass spectrometry. Then we sampled above the treatment plant, at the effluent outlet, and downstream of the effluent to determine pre-effluent and post-effluent drug concentrations. We are currently using this preliminary data to investigate how common endocrine disrupting, anti-inflammatory, analgesic, and anti-anxiety drugs may potentially affect the aquatic ecosystem of the endangered Chasmistes liorus, commonly known as June sucker.

Laurel Thompson, Utah Valley University Life Sciences Breast cancer is divided into subtypes which are defined by their proteomics, histology, and genetic expression profile. Current methods, therefore, are aimed at testing these, and include DNA microarrays, immunohistochemical staining, and proteomic analysis. These methods are effective classifiers, but are not easily transferable to real-time clinical applications, such as the determination of cancerous status during operation or when taking a biopsy. The determination of molecular subtype by other means would be a significant advancement in cancer detection and treatment. We have made some preliminary studies that suggest high-frequency ultrasound may be sensitive to variations among the cancer subtypes as manifest in cell cultures through their cytoskeletal protein structure, which has a distinct spectral signature. The object of this study was to explore the basis for this variation through a combination of experimental and theoretical analysis. We used first-principal modeling methods and compared the model spectra generated from these to spectra obtained in the cell culture lab. Variations in bulk modulus, cell position and size were modeled and combined with experimental spectra in principal component analysis (PCA), and the Euclidean distances between each principal component of the experimental were found as they relate to the theoretical principal components. A graphical method similar to heat maps used for gene expression profiling was then developed to display the relative distances (similarities) between spectra. The program was tested by comparing experimental spectra of three breast cancer cell lines to model spectra. The results indicate the properties and thus molecular subtypes of breast cancer cells could potentially be determined by comparing their measured spectra to model spectra using a feature classification program such as PCA and that these classifying features can be displayed in a convenient graphical representation according to their spectral similarities.

Austin Spence, Utah State University Life Sciences This study investigated the physiological effects of localized habitat disturbances around two well-studied hibernacula of the wandering gartersnake Thamnophis elegans. After three years of monitoring snake morphology and physiology at several hibernacula, several disturbance events occurred, including log clearing, stream bank disturbance, and vegetation removal. Individuals from three populations, two with disturbed hibernacula and one control population with no disturbance, were collected during the spring emergence immediately following the disturbance. Blood samples were collected upon capture and following a uniform stressor to measure baseline and post-stress physiological conditions. The samples were analyzed using a radioimmunoassay to measure corticosterone levels and a bacterial killing assay to measure innate immunocompetence. Baseline and post-stress corticosterone levels were higher in both populations with disturbance events compared to the control population. The bacterial killing ability of the site with the most anthropogenic activity was lower than the control site, indicating immunocompromise. Data are currently being analyzed to assess differences within the same populations between years with and without disturbance events. Pre-disturbance data are a rare and useful commodity and allow us to facilitate a better understanding of the various effects of anthropogenic change on natural populations. This study was funded through the Undergraduate Research and Creative Opportunity Grant from Utah State University.

Dani Peterson, Brigham Young University Life Sciences Due to its long and complicated trajectory through the cranium, facial nerve VII (CN VII) can be damaged in surgeries, sometimes resulting in facial muscle paralysis. Surgical removal of acoustic neuromas and parotid tumors, in addition to surgical repair of the temporomandibular joint disorder are associated with a risk of damage to CN VII. In addition, insertion of auditory implants can damage the nerve, as can improper stimulation to the nerve after the implantation has occurred. We will create a three-dimensional (3D) model based off of data from dissection of the nerve in a human cadaver in order to give physicians a greater in vivo knowledge of the pathway of CN VII. We have dissected the lateral side of the right half of the head to the level of the parotid gland, identified the parotid plexus of CN VII, and followed its five branches. In addition, we are currently following the nerve through the internal auditory meatus on its pathway through the temporal bone. In preparation for the modeling MicroScribe technique described below, we have imaged the head using Magnetic Resonance Imaging (MRI) at BYU. These images will be used as a template for the nerve reconstruction model. After completing the dissection, we will track the nerve trajectory using a MicroScribe 3D Digitizer. The MicroScribe technique is used to create 3D computer models of any physical object. The user sets reference points and uses the stylus to trace data points of the object’s contours. Our final product will be a 3D spatial computer mapping of CNVII, as well as a mapping of the skull, parotid gland, and other landmarks to put the nerve model into context. We hypothesize that with our approach and MicroScribe technique, we will be successful in creating an accurate model of CN VII in the head.

April McMurray, Brigham Young University Life Sciences Diabetes Mellitus Type 2 (DMT2) is a lifestyle-related disease where the body does not produce enough insulin or the cells are unreceptive to it, and it is now the most common form of diabetes. Individuals who do not control the disease can suffer serious complications such as limb amputation, damage to the eyes, kidneys, nerves, heart, and it can be very costly. This problem is particularly serious in Tonga; the prevalence is almost twice as high as that in the United States. The purpose of this research project was to determine to what extent the cultural, economic, and educational factors contribute to such high prevalence. In May I traveled to Tonga with the nursing students from Brigham Young University to conduct my research. I distributed surveys to patients and medical staff in the diabetes clinic in the Vaiola hospital in Nuku’alofa, Tonga. The surveys had questions related to their socioeconomic status, understanding and attitudes of diabetes, as well as patient management practices. While I was there, I also kept extensive field notes on observations related to my research, which provided supplemental information regarding the Tongan lifestyle that was difficult to gather from the surveys. Preliminary analysis indicates that there has been a very small, positive shift in understanding and attitudes towards DMT2, but economic- and culture-based habits still impede Tongans from managing the disease effectively. There were several limitations to this study: small sample numbers, lack of resources, some resistance from Tongan medical personnel, and particularly cultural barriers made it difficult to gather enough information to come to significant conclusions. However, the research does give insight concerning potential future studies and interventions to help the people of Tonga treat this disease.

Jacob Bassett, Utah Valley University Life Sciences Many discoveries regarding the complex interplay between biological pathways within a cell begin with attempts to link new observations to scrupulously studied cellular mechanisms. Our lab is studying the soluble inter-mitochondrial space protein mdm35, which has been observed to facilitate the function of ups1 & 2 proteins, as they in turn regulate the mitochondria’s phosphatidic acid metabolism. In addition to this phenotype, our lab has observed a copper resistance at 0.17% on YPD plate and 0.14% in liquid cultures of S. cerevisiae lacking the mdm35 protein, when compared to the Wild Type strain. Our investigation measures the levels of expression in the cup1 and ctr1 promoters in an attempt to link this observation to a potential mechanism contributing to this resistance.

Sara Fauver, University of Utah Life Sciences We know that novel genetic variants have driven evolution for millions of years and that natural selection favors phenotypes most suited for survival, leading to the enormous diversity of life we see today. However, what remains unclear, are the patterns of mutations that lead to large phenotypic changes. For example, do mutations in a single gene of large effect lead to morphological changes more often than numerous mutations in genes of smaller effect? Also, do these mutations occur more often in protein coding regions or regulatory regions of DNA? Finally, are the same genes or gene pathways used repeatedly across lineages when parallel phenotypes evolve?

Daniel Hall, Brigham Young University Life Sciences OBJECTIVE(S): Predictive analytics (PA) is increasingly being used in the delivery of healthcare. Whether PA can improve patient handoffs on a busy surgical service is unknown. This study aims to determine if predictive models for acute cellular rejection (ACR) episodes and biliary complications after orthotropic liver transplantation (OLT) can be built in order to improve patient care.

Spencer Alexander, Dixie State University Life Sciences Antibiotic resistance has increased with each new developed medication, creating new problems as bacteria become more difficult to defeat. Some of these bacteria are resistant because they can excrete an extracellular polymeric substance known as a biofilm. The polysaccharide-based biofilm matrix allows the colony to communicate, absorb nutrients, and exchange genetic material giving it an advantage in possible resistance through plasmid exchange. At present, there are no effective antimicrobial agents that can safely treat and prevent resistant bacteria like ORSA. Biofilms have a negative impact ranging from human pathogenesis down to economic expenses. In order to break down established biofilms, we utilized newly developed organic salts known as ionic liquids. These novel liquids have been observed to prevent bacterial colonies and biofilm formation, possibly by introducing intermolecular interactions that disrupt the chemical bonding in biofilms. The morphology of the microbes was characterized and observed to determine the effect of the ionic liquids on biofilms. Inhibition studies were also performed to determine antimicrobial efficiency of the ionic liquids as a function of organic structures. These novel ionic liquids provide an unprecedented, effective and efficient method to combat resistant bacteria, which could have tremendous impacts in achieving sterile environments in medical and remote settings.

Yonic Michaca, Utah Valley University Physical Sciences It is well known that some of the worst air pollution in the country each winter is found along the Wasatch front in northern Utah. This study examines the effects of environmental pollutants on the production of the novel compounds produced by the endophytes found in Rumex crispus. The Rumex crispus plant was selected due to its natural medicinal uses. It is anticipated that environmental pollutants have an effect on the production of bioactive compounds in order to protect their plant host from foreign pathogens. The theory is that the more stressful environment a plant lives in, i.e. desert climates, high altitude, and man-induced stresses such as pollution, the more bioactive compounds the endophytes produce as a response to protect their plant host. This study analyzes the effects of environmental pollutants along the Wasatch front on the production of novel bioactive compounds produced by the endophytes found in Rumex crispus. Plant samples are also collected from sites near the Wasatch front, but they are not exposed to the same amount of air pollution to be used as a control.

Ting Ruan, University of Utah Physical Sciences Cardiovascular complications are more prevalent in patients with diet-induced obesity and type 2 diabetes. Both of these conditions are associated with elevated levels of free fatty acids (FFAs). Elevated FFAs might precipitate cardiovascular complications by disrupting endothelial nitric oxide (NO) synthase (eNOS) enzyme function. The physiologically abundant saturated FFA palmitate decreases eNOS phosphorylation at serine 1177 (p-eNOS S1177) in a ceramide and protein-phosphatase 2A (PP2A) -dependent manner. p-eNOS S1177 is a positive regulatory site on the eNOS enzyme. As such, p-eNOS S1177 to total eNOS can be used as an estimate of eNOS enzyme function. We sought to determine the extent to which two other phosphatases that are abundant in the cytosol i.e., protein phosphatase 1 (PP1) and protein phosphatase 2B (PP2B) might contribute to palmitate-induced reductions in p-eNOS S1177 to total eNOS. Bovine aortic endothelial cells (BAECs) were treated for 3 hours with 500 uM palmitate or vehicle in the absence and presence of the PP1 inhibitor tautomycin (3 uM). p-eNOS S1177 to total eNOS was assessed using immunoblotting procedures. Palmitate-induced reductions (30±3%, p<0.05, n=3) in p-eNOS to total eNOS were similar in the absence and presence of tautomycin. These data indicate that PP1 does not contribute to palmitate-induced disruption of eNOS enzyme function. Next, BAECs were treated for 3 hours with 500 uM palmitate or vehicle in the absence and presence of the PP2B inhibitor cyclosporine (100nM). Palmitate-induced reductions (31±4%, p<0.05, n=3) in p-eNOS to total eNOS were similar in the absence and presence of cyclosporine. Taken together, these data suggest that neither PP1 nor PP2B contribute to palmitate-induced reductions in p-eNOS S1177 to total eNOS.

Frederickk Sudbury, University of Utah Physical Sciences Triphenylphosphine stabilized gold nanoparticles were synthesized using a method developed previously by the Jennifer Shumaker-Parry group. Aminated silica colloids were obtained from the Ilya Zharov group. The gold nanoparticles were adhered to the silica colloids using a method developed for another purpose by the Zharov group. The presence of the particles on the silica colloids was confirmed using both scanning and transmission electron microscopy. The catalytic activity of the gold nanoparticles both with and without adhesion was investigated. It was found that free nanoparticles had significant catalytic activity, in agreement with previous research. Gold nanoparticles attached to silica colloids do not seem to exhibit the same level of catalytic activity as the free particles. It was also found that not all the gold nanoparticles adhered to the surface of the silica particles. Experiments were conducted to determine if there was a critical concentration of silica colloids that would allow for high levels of adhesion of the gold nanoparticles. It was found that only by adding a significant excess of silica colloids to the solution can all the gold nanoparticles be removed from the solution. It was also found that the silica can be saturated with gold nanoparticles by including a small amount of them in a very concentrated gold nanoparticle solution. Further studies for functionalization of the silica colloids and the ability to physically isolate the silica with gold nanoparticles from free nanoparticles are currently in progress.

Kip Brower, Utah Valley University Physical Sciences Juniperus osteoperma has been reported to exhibit beneficial pharmacological activity against symptoms of diabetes, as a traditional treatment for kidney ailment and the essential oil of juniper berries has also been subject to investigation for antioxidant activities. Endophytes, particularly endophytic fungi, have produced numerous novel bioactive compounds, several of which are currently used as antifungal, anti-bacterial, and anti-cancer agents in organisms other than their plant hosts. This investigation looks into the possibility of endophytic fungi being a means of producing compounds that may be a functioning agent in antioxidant, antifungal, or hyper/hypoglycemic activities of Juniperus osteoperma. The investigation involves samples of Juniperus osteoperma collected from multiple different locations within Utah, and isolates the resident endophytic fungi.

Stephen Erickson, Brigham Young University Physical Sciences “The spherical protein ferritin has often been used to fabricate nanoparticles of various shapes and compositions with its walls. Ferritin occurs naturally with a ferrihydrite (FeOOH) mineral core, but it has also been used to synthesize nanoparticles of several other semiconductors. While the methods for creating these nanoparticles within ferritin are well established, the characterization of such nanoparticles is not. Previous studies on native ferrihydrite core ferritin disagree on the band gap, giving values anywhere from 1.1-3.5 eV, depending on the method. We have used absorption spectroscopy to measure these band gaps with an unprecedented accuracy of up to .01 eV. This method also allowed us to determine that ferrihydrite nanoparticles are indirect gap semiconductors. By employing this method on particles of various sizes, we have shown the effects of quantum confinement, resulting in variations in the band gap. We also provided the first ever direct evidence that ferritin works to crystalize its core with time, an effect that has long been theorized but never observed. By characterizing the effect of size and time on nanoparticle band gap, we have shown the potential for selectively tuning that gap. This opens up a world of possible applications in light harvesting and photo detectors. By controlling the band gap, we will be able to select which wavelengths of light are absorbed, allowing for full spectrum photovoltaic cells and wavelength specific optical detectors. Future studies will focus on nanoparticles of other metal hydroxides and various anion replacements to further expand our tunable range of band gaps.

Byron Millet, Weber State University Physical Sciences The relative solvolysis rates of norbornenyl derivatives have been of interest. To date, only one other norbornenyl compound has been found that solvolyzes slower than anti-7-norbornene. It has been suggested that through-space interactions between π-bonds plays a significant role in stabilizing the carbocations. However, through-space interactions do not account for the similar observed solvolysis rates of nearly identical compounds both with and without adjacent π-bonds. Studies have shown that the stability of these compounds is significantly affected by σ-bond interactions. We report on our current computational study of the relative energies of several norbornenyl compounds with and without adjacent π-bonds to evaluate both π-bond and σ-bond effects on the stability of the norbornenyl cations. The effect of a ketone functional group on the relative stability of the carbocation is also analyzed. The literature solvolysis rates of the various norbornenyl derivatives and the stability of their respective cations is compared. With some exceptions, a correlation was found between the stability of a derivative’s carbocation and its respective rate of solvolysis.

Jeremiah Rundall, Utah Valley University Physical Sciences Utah Valley University has a long-term program of groundwater development in the Trans-Mexican Volcanic Belt in the state of Guanajuato, Mexico, in cooperation with Choice Humanitarian. Thus far, we have drilled a successful 50-m well in basalt and an unsuccessful 22-m well in rhyolite. Well sites have been chosen based on spring locations and fracture traces (linear features as seen on aerial photos). The objective of this study is to measure and model the geoelectric and magnetic characteristics of fracture traces as a means of determining which fracture traces are most indicative of actual fractured rock. Thus far, studies have been carried out on 20 fracture traces in rhyolite in the Trans-Mexican Volcanic Belt, six fracture traces in rhyolite/diorite/monzonite of the La Sal Intrusives in the La Sal Mountains of Utah, and one fracture trace in rhyolite of the Soldiers Pass Formation west of Utah Lake. The total magnetic field has been measured at about 1-m spacing along profiles perpendicular to fracture traces using the Geometrics G-856 Proton Precession Magnetometer. Geoelectric soundings for resistivity, chargeability and self-potential have been carried out both on and off fracture traces using the Iris Instruments Syscal Junior Resistivity Meter with the Schlumberger array parallel to the fracture trace at spacings in the range AB/2 = 2.29-137 m. Geoelectric profiles have been carried out perpendicular to fracture traces (array parallel to the fracture trace) at spacings AB/2 = 15.2 m and 137 m. Geoelectric and magnetic modeling is being carried out using the 1X2D-GM Magnetic Interpretation Software and the 1X1D Sounding Inversion Software. Preliminary results indicate that some fracture traces are associated with both magnetic and electrical resistivity lows consistent with intermediate depth (~ 50 m) fractured rock, although not all fracture traces have these characteristics. Further results will be reported at the meeting.

Alexandra Kent, University of Utah Physical Sciences Prescription drug overdose and abuse is a leading cause of death in the United States. It is a serious issue and has become increasingly problematic as opioids are being prescribed at a higher frequency. For this reason, fast, accurate detection of small drug molecules is crucial. The current standard for use in clinical drug detection is an enzyme-linked immunosorbent assay (ELISA) that uses a series of antibodies to bind to the target drug and enable quantification via a colorimetric output. However, the antibodies used in an ELISA often cannot distinguish between similar molecules. Aptamers are short sequences of DNA that have emerged as a promising alternative to antibodies, as they are generated in vitro, where negative selections can be used to increase target selectivity. These aptamers can be cleaved to make split aptamers that only assemble in the presence of the target small molecule. One inherent problem of this system is the need for split aptamers that are selective for their small molecule targets. While there are many known aptamers, there are only a few known split aptamers that bind small molecules. Separating aptamers with a privileged, three-way-junction structure provides a reliable method to generate new split aptamers.

Jazmin Myres, Brigham Young University Physical Sciences In scan-based array measurements, stationary reference sensors are needed to temporally correlate the different measurement scans and produce coherent complex pressure fields. Because the number of references required increases with the number of subsources contributing to the sound field, an extended, partially correlated source comprising many ill-defined sources can result in significantly increased measurement complexity. A different approach to creating spatiotemporally coherent pressures is demonstrated here. Scan based measurements of a partially coherent line source have been taken in an anechoic chamber. This experimental data has been used to explore “stitching” together a complex pressure field by spatially overlapping measurement scans instead of using separate reference channels. Various methods of stitching have been explored and the most robust method identified. Unwrapping of intrascan phases is first accomplished with a two-dimensional phase unwrapping algorithm. Individual scan positions are then stitched together using median phase differences between multiple adjacent scans to create coherent planes of data. Amplitude-stitching is done by averaging across scans and preserving the integrated squared pressure across the overall aperture. This method has been verified using reference microphones. This stitching method has been applied to scan based measurements of a military aircraft, exhibiting its effectiveness dealing with a partially correlated complicated source. This method works well for low-frequency jet data, where there is not a ground-based interference null creating a physical phase discontinuity. This technique provides direction for efficient experimental design for scan-based array measurements of extended sources. [Work sponsored by ONR.]

Brandon B Davis, Utah Valley University Physical Sciences A recent study (Ferreira 2013) examined concentrations of fluvial As and transition metals associated with As in Provo and American Fork Rivers, which flow westward across the Wasatch Range and drain into Utah Lake. Within Utah Valley average fluvial As for Provo River (As= 0.342 mg/L) and American Fork River (As= 0.152 mg/L) exceeded the EPA standards for freshwater streams for acute exposure (As= 0.340 mg/L) and chronic exposure (As = 0.150 mg/L), respectively. The objective of this study is to determine whether elevated levels of As and other heavy metals also occur in shallow groundwater in Utah Valley. The objective is being addressed by analyzing water samples from the “backyard wells” in Utah Valley, shallow (depths < 10 m), hand-dug wells which many residents maintain as their "emergency water supply" or for small scale agriculture. Since nearly all backyard wells are unregistered, they are being sought through conversations with water departments, real estate listings and local residents. Water and sediment samples are also being collected from American Fork River, Hobble Creek, Provo River and Spanish Fork River, which drain into Utah Lake. Samples are being analyzed for nitrate, phosphate and sulfate using the Hach DR-2700 Spectrophotometer, while the PerkinElmer Optima 8000 ICP-OES is being used to measure for As, the transition elements Co, Cu, Cr, Fe, Mn, Ni and Zn, and other associated elements Ag, Cd, Pb and Ti. Preliminary results indicate that shallow groundwater As in the American Fork watershed is much lower (mean As= 0.0022 mg/L) than fluvial As and an excellent negative correlation (R2 = 0.83) between groundwater As and Ti. It is suggested that TiO2, which may originate in the Cottonwood Stock in the American Fork watershed, may play a role in the demobilization of groundwater As. Further results will be reported at the meeting.

Tyler Jones, Brigham Young University Physical Science This project takes advantage of an inexpensive integrated circuit to create a wavelength meter. The circuit has an array of photodiodes, each of which has an optical filter of a different color. The filters cause each diode to respond differently to different wavelengths of light. The relative amplitudes of the signals are then used to compute a wavelength based on known responses for various wavelengths recorded in a calibration stage. The project focuses on improving precision of the hardware and developing an algorithm to do the computation.

Stephen Ruegg, University of Utah Physical Sciences The Black Peaks Formation (BPF) from the Tornillo Group in Big Bend National Park (BBNP) is comprised of a series of stacked paleosols and sandstone channels deposited by a fluvial system in a sub-tropical intramountain basin during the Laramide orogeny. Paleosols constituting the BPF display alternating drainage and development conditions. The BPF is bounded by the Late Cretaceous Javelina Formation and the Early Eocene Canoe Formation and is therefore thought to be of Paleocene age (65.5-55.8 million years ago). The BPF is an interesting target for paleoenvironmental reconstruction because little data have been generated from the Paleocene for sub-tropical regions, limiting the validation of global climate model predictions in these regions. However, previous attempts to resolve the age of the formation using biostratigraphy, magnetostratigraphy and chemostratigraphy gave ambiguous results limiting the potential of the BPF for paleoenvironmental reconstruction. We analyzed the carbon isotope ratio (δ13C ) of carbonate nodules collected from pedologically distinct paleosols throughout the BPF. δ13C of carbonates nodules found in reduced black paleosols are systematically 3-4‰ lower than δ13C from non-black paleosols from neighboring stratigraphic intervals. We hypothesize that this isotopic difference is related to the lower contribution of atmospheric CO2 to soil CO2 in water-logged and/or poorly drained black soils. Large-scale stratigraphic patterns of carbon isotope variations in carbonate nodules from non-black paleosols throughout the BPF strongly resemble well-documented secular changes in δ13C values of marine carbonates for the Paleocene. Several recognizable features are present in both curves, including the Paleocene Carbon Isotope Maximum (PCMI) and possibly the Late Danian carbon isotope excursion (LDE). These features provide a new basis for correlation of the BPF to the global geological timescale, and suggest that the Formation preserves a 6 million year record of deposition and paleoenvironmental conditions spanning the majority of Paleocene time (63-57 million years ago).

Christopher Mansfield, Westminster College Physical Science The method currently used to measure monomethylmercury (MMHg) in natural waters involves a lengthy distillation step in order to remove certain ions and dissolved organic matter that have been reported to interfere with the subsequent reaction in which MMHg is ethylated. It has recently been reported that the analysis of MMHg in seawater by direct ethylation was successfully carried out, thus removing the day-long distillation. However, the effect of many potential interfering ions and compounds on this method have not been characterized, and it has not been tested for use in natural freshwaters or the hypersaline waters of the Great Salt Lake. Thus, these became the two goals of this project. We found that optimal ethylation conditions included buffering samples to pH 4.0-4.1, reducing the amount of sodium tetraethylborate, adding EDTA to complex interfering trace metals, and adding chloride to overcome interferences by thiols and organic matter. We have since employed this improved method to analyze MMHg in natural water samples from fresh and saline lakes, achieving > 90% MMHg spike recoveries without the use of distillation.

Jasmine K Bishop, University of Utah Physical Sciences In my presentation, I will discuss the method of creating insulating gaskets for high pressure experiments using diamond anvil cells. In standard diamond anvil cell pressure experiments, metal gaskets are typically employed. However, in order to study the electric and magnetic properties of a sample without worrying about interference of the metal gasket itself, insulating gaskets are used. The insulating material needs to be ductile and yet hard enough to maintain a certain level of structure. Epoxy is ideal for ductility but is not hard enough to maintain a diamond indent under pressures generated by diamond anvil cells. When mixed with a diamond powder however, the mixture is both ductile and structurally sound. In this study we have modified the previous methods of insulating gasket preparation by addition of a metal sheet to increase the strength and ductility of the gasket. The part of the gasket that is metal can be used as an electrical lead to measure sample’s conductivity. In this design the mixture of diamond and epoxy is applied to a thin 100 micrometer metal sheet and a focused beam of high power IR laser is used to drill a hole in the gasket . I will describe the design of the optical path and the details of the gasket preparation and will present some of the gaskets that I have made.

Stephanie Bartlett, Utah Valley University Physical Sciences Ephemeroptera, commonly referred to as mayflies, are found throughout the world. Within the order of Ephemeroptera resides a superfamily, Ephemeridea, commonly called burrowing mayflies. This common name was acquired due to certain physical and behavior characteristics present as nymphs. Ephemeridea nymphs live in the silt of aquatic environments and have adaptations for burrowing which include strong legs, as well as mandibular tusks. One exception to this is the family Behningiidae, which burrow, but lack the commonly associated mandibular tusks. Morphological data supported Behningiidae as sister to the other tusked burrowing mayflies, indicating that first burrowing behavior evolved and was later followed by the development of tusks(McCafferty; 1975 and 2004). While morphological data provides important insights into the evolution and phylogeny of mayflies, the development of molecular phylogenetics offers new contributions when determining evolutionary relationships within this superfamily. Objective: The purpose of this study is to investigate the relationships of the families of burrowing mayflies in order to test the hypothesis of tusk evolution. Methods: The specimens were acquired from collection efforts and colleagues. For each specimen the following laboratory procedures were carried out: DNA extraction, gene amplification via polymerase chain reaction, visualization via gel electrophoresis, and DNA sequencing. The genes targeted for sequencing included 12S mitochondrial rDNA, 16S mitochondrial rDNA, 18S nuclear rDNA, 28S nuclear rDNA, H3 nuclear protein coding, and CO1 mitochondrial protein coding. Data was also acquired from Genbank in order to augment missing data. Taxon sampling consisted of around 20 ingroup and 5outgroup taxa. Phylogenetic relationships were estimated using Maximum Parsimony, Maximum Likelihood, and Baysian methods. Conclusion: The families Behningiidae, Potamanthidae, Palingeniidae were supported as monophyletic. Behningiidae nested well within the other burrowing families. Hence, tusks evolved and were subsequently lost in the family Behningiidae even though it retained the burrowing lifestyle in the nymph.

Mattie Jones, Dixie State University Physical Sciences New methods aimed at forensic analysis of sensitive, minute samples are critical to the intelligence community. In particular, successful extraction of dyes from materials found at crime scenes will provide innumerable benefits for matching, identifying, and finding origins of these materials and dyes. Current methods of isolating components of samples by their unique chemical properties are lengthy and often destroy important forensic evidence. Ionic liquids possess the necessary chemical properties to ensure efficient extractions, while maintaining the forensic signatures of the original materials. They also provide a one-pot approach that avoids intermediate species and increases analyte yield while extracting and separating constituents in a more efficient manner. By combining traceable dyes with an ionic liquid, the versatility of single-component extraction-separation-identification was demonstrated. Analysis using absorption and fluorescence spectroscopy validated complete extraction and recovery of trace analytes. Following extraction and isolation, identification by infrared spectroscopy has provided evidence of preserved quality and complete separation of material and dye. This novel approach to forensic analysis is advantageous particularly when sample sizes are extremely limited, but it can be readily scaled to larger applications. Developing a simple and affordable method of achieving specific molecular interactions provides a solution for often unidentifiable evidence in crimes. Harnessing the versatility of ionic liquids in a high-yielding recovery and efficient single-pot methods will enhance forensic abilities for the intelligence community and forensic investigators.

Phillip Lundgreen, Utah State University Physical Sciences By developing a low-noise, high-voltage battery power supply, system noise has been reduced, increasing accuracy of conductivity measurements of highly disordered insulating materials. The method involves a simple parallel plate capacitor setup with the sample sandwiched between electrodes, a voltage potential applied to one electrode, and a measurement device applied to the back electrode measuring current. Previous methods involved use of a commercial power supply with a claimed low noise and high linearity, but with a low AC output ripple. At high voltages (1000 V), however, the noise became apparent in the readings and an unacceptable uncertainty was introduced in our precision conductivity measurements. Through the use of a stable dc battery high-voltage power supply, we were able to reduce noise in current measurements and achieve a more accurate measurement of conductivity for various samples.

Zachary Anderson, Brigham Young University Physical Sciences Correlation analysis is useful in extracting spatiotemporal relationships between signals and can be used to examine features of near-field jet noise for source properties. Characteristic correlation envelopes determined by Harker et al. [JASA 133, EL458 (2013)] can be used to relate correlation lengths to fine and large-scale turbulent structures. As an extension, cross-correlation shows spatial variation in jet noise and further reveals the transition between short (fine-scale) and long (large-scale) correlation lengths. These analyses are applied to a military jet dataset of a ground based linear microphone array positioned 11.6 m from the jet axis. Correlation analyses over multiple engine conditions and observation directions are reported. In particular, a maximum correlation coefficient greater than 0.5 exists over a range spanning multiple wavelengths in the region of greatest overall sound pressure level at military power. [Work supported by ONR.]

Ian Sohl, University of Utah Physical Sciences Current software in the High Altitude Water Cherenkov (HAWC) Telescope data collection system only triggers saving of events that pass a specified number of hits and energy. Our analysis of the untriggered (and unsaved) data will identify the number of potentially significant events that are bypassed by the existing software. By the application of a sorting function onto current Monte Carlo generated data, we can categorize incoming events into various types of particles, primarily muons, while also filtering out randomized noise from the photomultiplier tubes used in HAWC. Due to the relatively low rate and energy of the photomultiplier tube noise, many of the significant events for the untriggered set are potential useful particles. Our triggering algorithm, based on the energy, timing and saturation of the tanks is primarily focused on separating muons from the bulk of data. These raw rate data for small events are a relatively unexplored area for HAWC and measurements can be useful for a variety of calibration tasks for the telescope. They can be useful for understanding the impact of the atmosphere on the telescope’s data collection, as well as the triggering of the photomultiplier tubes through secondary sources. This untriggered data can also be used in a variety of useful forms not directly related to HAWC’s primary usage goals, primarily solar physics. Due to the relatively low energy and hit count from solar events, most of the data are thrown away by the triggering algorithm.

Mallory Millington, University of Utah Physical Sciences Groundwater in the western United States is a limited and important resource for agriculture, industry, and residents alike. Knowing the movement of groundwater is critical to understanding the potential of groundwater contamination from human activities. While groundwater flow is difficult to quantify, it typically recharges in high elevation and discharges towards lower elevation. The first commercially-approved tar sands mine in eastern Utah is located on a ridgetop in the PR Spring area within the high plateaus on the south rim of the Uinta Basin. To evaluate the potential of groundwater contamination due to tar sands mining a study was conducted to understand groundwater flow in the PR Spring area, specifically the canyon directly south of the tar sands mine called Main Canyon. Water samples were taken from four groundwater springs at elevations ranging from 7040 to 8040 ft in or near Main Canyon. Water quality measurements taken in the field showed that the lower springs exhibited higher conductivity (900 vs. 636 μS/cm) and lower dissolved oxygen (30% vs. 88% saturation) than the higher elevation springs. This suggests that the lower springs have had a greater amount of water-rock interaction and so are chemically more evolved than the springs at higher elevations. SF6 age dating indicated that the high elevation springs are younger than the lower elevation springs, 5.5 vs. 16.0 years since recharge respectively. The field parameters and SF6 age data all indicate that higher elevation springs are younger and less chemically evolved than the water at lower elevations. This indicates that the springs in Main Canyon are sourced from local recharge at the ridgetops. Given these findings the tar sands mine should consider taking preventative measures to protect groundwater resources.

Andrew Fletcher, Utah Valley University Physical Sciences Springville, Utah, is known for its high water table and many freshwater wetlands and springs, which gave rise to the name of the city. Flooding of barns is a common problem among farmers in western Springville, which is just east of Utah Lake. These frequent barn floods are anecdotally linked to high-intensity precipitation events and the common use of flood irrigation. The objective of this study is to determine the cause of barn flooding and to make recommendations for mitigation of barn flooding for individual farmers with whom we are working in this area. The objective is being addressed first by using ArcGIS to determine the watershed of each barn and the NRCS (Natural Resource Conservation Service) Web Soil Survey to map hydrologic soil groups within each watershed. Results from the larger-scale Web Soil Survey will be supplemented with measurements of soil hydraulic conductivity using the SoilMoisture Equipment Model 2800K1 Guelph Permeameter. The above data will be used in the NRCS Curve Number Method to estimate the volume of surface runoff expected in a 100-year 24-hour precipitation event. The objective is also being addressed by installing shallow, hand-augured wells for monitoring the depth to the water table during high-intensity precipitation events and flood irrigation events. The results will be used to develop recommendations for a combination of (1) installation of French drains for diversion of stormwater (2) modification of current irrigation methods (3) pumping and diversion of groundwater. Results and specific recommendations for individual farmers will be presented at the meeting.

Nils Otterstrom, Brigham Young University Physical Sciences Filtered photodiodes show potential as inexpensive laser wavelength meter. Photocurrents are measured digitally. The photocurrent is digitized using externally controlled integration times to achieve the highest precision possible from the digital to analog converters on the photosensor chip. Using an algorithm we’ve developed and calibrated intensity curves, we can precisely calculate wavelength from the output of the different photodiodes. Limitations due to etaloning from reflections off of the surfaces of the filters were analyzed and effectively mitigated, allowing the device to achieve high precision with a stability of 0.102 nm over several hours.

Brittney Thaxton, University of Utah Physical Sciences There are many undocumented shorelines seen in Utah. As many as 30+ shorelines have been identified along the edges of the basin throughout the state. The purpose of this study is to characterize undocumented shorelines and identify potential evidence for tsunamis that might have occurred in Lake Bonneville thousands of years ago in areas such as Little Mountain, Stansbury Island, and Promontory Point, Utah. Lake Bonneville existed 32-10K years ago and was influenced by the Wasatch fault which was active as early as the Miocene. Scarps of this age are common and range between 15-20 feet in height (Machette, Personius, Nelson, Schwartz, Lund 1991; Dinter, Pechman, 2004a and 2004b). Several faults beneath Lake Bonneville could have produced tsunamis. The more water that is displaced the greater the tsunami will become and leave a greater impact onshore (Dutykh and Dias 2009). The East Great Salt Lake fault cuts NS across Bear River Bay east of Promontory Range. This fault line is an excellent candidate for causing a tsunami during the Lake Bonneville highstand because it is beneath the Great Salt Lake meaning it would have uplifted the entire water column of Lake Bonneville and since it is also close to the Promontory Range, it is likely it would leave tsunamite evidence along the shore. The fault rupture interval is between 3,000 and 3,500 years meaning fault ruptures could produce a tsunami during the lake’s high stand. Tsunamite is the term used for deposits related to tsunamis. The tsunamite features found along the shorelines will be similar to sedimentary features such as normally graded sand, mudstone clasts, and other gravel deposits that are out of place with the known shorelines (Shanmugam 2006). This is a unique opportunity to discover ancient tsunami evidence in Utah, a topic that has yet to be pursued.

Nicholas Labrum, Utah State University Physical Sciences Increasing concerns on the anthropogenic climate change, rising global energy demands, and diminishing fossil fuels have urged the search of alternative carbon-neutral and sustainable energy resources, among which solar energy stands out as the most promising target since it is the largest exploitable resource. However, its nature of diurnal variation, intermittence, and unequal distribution requires efficient and cost-effective capture, conversion, and storage. Generation of chemical fuels, such as hydrogen, from solar energy input represents an appealing approach to meet this goal. An ideal scheme would tap hydrogen from the splitting of water with concomitant evolution of oxygen. Due to the nature of the four-proton and four-electron process, water oxidation is the bottle neck of the overall water splitting process. Nature catalyzes water oxidation using an oxygen evolving complex (OEC) in photosystem II. This project aims at mimicking the OEC to prepare and investigate bimetallic Mn-Ca catalysts for water oxidation catalysis. Calcium has been reported to be critical in water oxidation by OEC, however its functional role has not been well studied. By positioning a calcium atom in the second coordination sphere of manganese in molecular scaffolds, we are able to systematically study the functional role of calcium at the molecular level. Our project will prompt the development of water oxidation catalysis and benefit artificial photosynthesis at large.

Greg Carling, Brigham Young University Physical Sciences Wyoming the second most glaciated state in the lower 48 United States has seen drastic changes in the size of its glaciers. Glaciers in high elevation ecosystems of Grand Teton National Park are not anywhere near to the size that they were 100 years ago. The glaciers continue to decrease in size every day. As the environment changes the glaciers change in size and can be affected by many factors in the environment. Deposition of particulate matter from the atmosphere into the glaciers occurs as pollution is becoming worse and more common. Studies done throughout the world have shown that glaciers can act as a source for mercury and other trace metal elements in high elevation ecosystems. Through the assistance of the UW-NPS Research Station Dr. Greg Carling of BYU and his team of graduate and undergraduate assistants retrieved 100 glacial melt water samples from the Middle Teton, and Teepee Glaciers and stream sites in Garnet Canyon, and from the Teton Glacier in the Glacier Gulch area. In the data analysis completed up until this point, concentrations of various trace elements have found in sample sites in close proximity to the Middle and Teton glaciers on the glacial moraine. We hypothesize that these glaciers act as a source for mercury and trace elements that can then be transported to lower elevation ecosystems within the Greater Yellowstone Ecosystem.

Tucker Chapman, Brigham Young University Physical Sciences The Provo River provides the opportunity to study three systems from low to high anthropogenic activity. Its headwaters are in an undeveloped area of the Uinta Mountains. The river then moves into a valley that is developing from an agricultural to an urban system. The lower portion of the river moves into the urbanized Utah Valley. These systems give the ability to study the changes in trace element chemistry from a variety of sources. Trace element data were collected during the 2013 water year including the spring snow melt. Correlation was analyzed among the different trace elements using multivariate statistics in order to discover trace element sources. The element loads were calculated using USGS Load Estimator (LOADEST) software. The study has implications involving the drinking water of >2 million people in the Utah and Salt Lake valleys and the changes that the shift from agriculture to urban is causing.