2014 Abstracts
Post Traumatic Stress Disorder in the Ancient Greek and Roman Soldier
Echo Smith, University of Utah Humanities Since the publication, in 1995, of Jonathan Shay’s Achilles in Vietnam, an increasingly popular view of PTSD in soldiers holds that modern day combatants experience the horrors of warfare in much the same way as did ancient Greek and Roman soldiers and that PTSD must have been just as prevalent in the classical world as it is today.
Paradise Lost: K. as Everyman in the Fallen World of Franz Kafka’s novel The Trial
Emily Simmons, Utah Valley University Humanities In Franz Kafka’s novel The Trial, the protagonist Josef K. is accused and ultimately punished for an unspecified crime of which he proclaims himself innocent. This paper examines Kafka’s novel through a religious studies approach—specifically, offering a reading of the Judeo-Christian myth of the fall of Adam and Eve, as exemplified in The Trial. I thus posit that K. is a type of post-fall Everyman who suffers the effects of the fall, attempts to restore himself to a Edenic state, but rejects the opportunity to be the fallen world’s savior and instead resigns humankind to continued suffering. To substantiate my argument I analyze four aspects of the novel. First, I examine K.’s conversation with the priest as they discuss the nature and origin of K.’s guilt. Next, I show that K.’s compulsive sexual behavior is his attempt to attain a spiritual completeness through sexual intercourse. Then, I find that as K. is suffering the effects of Adam’s fall, so too are the women suffering from the curse of Eve. Finally, I explore instances where K. exhibits similarities to the Biblical Jesus Christ, arguing that K. has the chance to perform a “savior” role but refuses. In addition to my analysis of the primary text, this paper also draws on contemporary scholars who discuss themes both Biblical and secular with an eye toward understanding K. and his relationship with women and the world around him. As a result of this analysis, I conclude that although K. is demoralized from the corrupt judicial system, maze of pointless bureaucracy, and hollow authority of the novel’s priest, he continues to seek, until the very minute of his death, salvation from the effects of the fall.
Germans and the Wild West: The Creation of German National Identity through Cold-war Era “Sauerkraut” Westerns
Romy Franks, Brigham Young University Humanities Following the Second World War, the adventurous westerns of 19th-century author Karl May resurfaced in Germany as wildly popular pulp fiction and dime novels. In subsequent decades, May’s literature inspired several successful film adaptations in the West, followed by alternative “Indian films” in the East. Karl May and the contemporary phenomenon of the global Western film genre tapped into many themes central to Germany’s long, tortured quest for a national identity. Post-war Germans looked to Westerns as a means for “projecting” a new image of Germany to the world-one that could help overcome the stigma of Nazism.
Master of Passion, Freed with Reason
Quinn Mason, Brigham Young University Humanities “Law is the highest reason, implanted in nature, which commands what ought to be done and forbids the opposite,” Cicero wrote, “This reason, when firmly fixed and fully developed in the human mind, is Law… Law is intelligence, whose natural function is to command right conduct and forbid wrongdoing.” In Bronté’s well-known novel, Jane Eyre, Jane is confronted with all she desires which is the love of Mr. Rochester, who is married under peculiar circumstances to crazy Bertha Mason. She is given the option of living with Mr. Rochester outside of marriage. However, Jane states, “I adhered to principle and law … [and] scorned and crushed the insane prompting of a frenzied moment”. My research focuses the role of natural law within the complicated situation presented before Jane Eyre and how she masters her passion, rather than being a slave to her desires.
Sowing a Legacy: The vision of Cesar Chavez
Haylee Jones, Dixie State University Humanities Cesar Chavez, who experienced poverty and exploitation by way of labor practices as an adolescent, became a man who committed himself to making a positive impact on the working conditions for immigrant farm workers in America. He left a fading footprint that others have been reluctant to follow. This paper will examine various role models like Juana Estrada Chavez, Chavez’s mother, and Dolores Huerta, Chavez’s closest associate, who were big influences in Chavez’s decision to become a leader in the civil rights movements as explained by author Kathleen Krull. Authors Susan Ferriss and Ricardo Sandoval elucidated on many of Chavez’s achievements such as the organization of the United Farm Workers (UFW), a group created to unionize immigrants, and the orchestration of nonviolent strikes and marches to expose the unfair treatment of multi-ethnic farm workers. Although Chavez persuaded California to sign contracts that discriminated against biased pay and working conditions, many immigrants still work longer, more strenuous hours than the average American to receive only $2.00 an hour and live in beat down shacks they are forced to call home. Cesar Chavez had a vision that should be remembered and re harvested among Americans everywhere in order to continue improving working conditions of all citizens, no matter where they are from.
Experiences in Education for Utah’s People of Color- A Neglected History
Christopher Wiltsie, Utah Valley University Humanities Low racial diversity in Utah leads to common assumptions about the lack of racial tensions. This has resulted in a general disregard for the history of civil rights struggles, specifically in education, for people of color in Utah. In the US as a whole, the 1970s featured both legal and social reform in issues of race and its role in education, but accounts from minorities in Utah tell a different story. This project will be a comparative history, analyzing oral histories regarding educational experiences of people of color that lived in Utah immediately after 1968 until 1980, within the context of the trends at the time throughout the United States. Extensive interviews will provide documentation regarding racial tensions and their effects on educational experiences and achievement. The synthesis of these accounts will help fill in the gaps that exist within Utah’s historical record. Obstacles in education for people of color during the 1970s will be discussed and compared to successes and failures throughout the United States.
Unearthing Bharat Mata: Utilizing An Ecocritical and Subaltern Focus to Comprehend Modern Indian Identity in English Literature
Mahreen Bashir, University of Utah Humanities A complicated social paradox humanity faces is assessing the union between the seemingly contradictory ideologies of “sustainability” and “growth” into one viable system. Adapting a merger between concepts such as: the retention of cultural values and social systems, industry, urban sprawl, modernity, sanitation, progress and the environment. This objective of this research project centers around using understandings of post-colonial theory to find new connections in South Asian identity through South Asian literature written in English, and the literature’s relationship to ecology. Specifically, it applies the nascent subaltern lens of a historiography that examines South Asia as both a post-colonial subcontinent, and an emerging “superpower,” in the rhetoric of a global economy, to understand a relationship between Indian people and India as place. Questions asked are posed in regard to the affects of orientalism and a cultural infiltration of seeing endemic knowledge to be degenerate in relation to Western academia; Indian-American identity; and the weight of the term “subaltern” through works by South Asian writers. Furthermore, the project uses an ecocritical lens on these works to extract how identity discourse and post-colonialism have created understandings of Indian identity within English literature. The anticipated outcome of this research paper is to create a greater understanding of both emergent academic lenses, and their application in understanding Indian culture and identity as portrayed by works written by Indo-American writers. This research idea was born out of my passion for both ecology and post-colonial studies that I have honed through my studies as an environmental studies and gender studies double major. The main objective is to investigate the plurality of identity, and the ramifications that identity, has on the establishment of more sustainable behavior to counter the impending global crisis the planet is facing in terms of lack of environmental stewardship, and subsequent social justice.
Armenian Coffee Houses in David Kherdian’s Homage to Adana: Negotiating Geographical, Generational, and Cultural Identities
Helen Makhdoumian, Westminster College Humanities Despite roughly a century of Armenian American literary production, the literary theory and criticism on this body of ethnic literature has only started to develop in the last few decades. David Kherdian is an Armenian American writer whose works range from poetry to prose, fiction to memoir, and translations to retellings of Armenian tales. My research focuses on David Kherdian’s poetry collection Homage to Adana, published in 1970. Previous critics have analyzed Kherdian’s poetry for themes such as childhood, familial relationships, self-discovery, and personal and collective memory. Some critics have read some of Kherdian’s poems as reflecting the loss of Armenian culture due to assimilation in the U.S. In contrast, this presentation analyzes the motif of the Armenian coffee house in select poems in Homage to Adana as a space where geographical, generational, and cultural identities are negotiated. Although on the surface these poems indicate a loss of Armenian culture, I argue that they actually indicate a continuation and adaptation of Armenian culture in the U.S. by the younger generation. To support my argument, my approach uses the theoretical lenses of New Historicism and Cultural Studies. I contextualize these texts with the history of the Armenian diaspora and immigration. Furthermore, I include the historical and cultural significance of Armenian coffee houses as well as the use of Armenian coffee in cultural knowledge sharing. In general, the themes I look for are references to the old country, representations of immigrant Armenian men and women, oral storytelling, and food traditions. Ultimately, this analysis reveals how the poems reflect the negotiation of passing on cultural knowledge. By both continuing traditions and adapting them for everyday lived experiences, Armenian culture will remain vibrant in diaspora.
The Bitch vs. the Ditz: The Perception of Female Politicians in American Print Mass Media
Lacy Culpepper, Dixie State University Humanities In the past century, women have made monumental progress in their presence and authority in American politics; unfortunately, print mass media outlets overall have not positively reflected those changes. In his work, On Rhetoric, Aristotle argues that a person’s character is the most effective method of persuasion, and as female politicians fall subject to the words of the media, their perceived character, and impact as a leader, depends heavily on the opinions of the writers and analysts of the various American print sources. Print media outlets tend to categorize notable female politicians into two categories: the bitch, who must abandon her well-rounded, understanding realm of femininity and adopt notions of an aggressive, haughty persona; or the ditz, who must heavily rely on the dated, stereotypical femininity that encourages beauty over brains and forsakes a hold of influence and legitimacy. This seemingly timeless application proves that, regardless of which category a female politician is assigned, such press pushes her politics aside and can have a serious negative impact on both her career and reputation. For this paper, I analyze the print treatment of Hillary Clinton and Sarah Palin during the 2008 presidential election to explain the rigid Bitch/Ditz classification of female politicians that sources including Time and People have assigned.
Battle-Wolf
Zacrey Hansen, Utah Valley University Humanities Within Old Norse myth lurks Hildolf, whose name means Battle-Wolf. He only appears in the Medieval Norse poems twice: in a name list of Odin’s sons in the Prose Edda, and then is off-handedly mentioned by Odin himself, in the Poetic Edda. Since the mythic stories make no other mention of Hildolf, the general scholarly consensus is that Hildolf is simply another of Odin’s many aliases. Indeed, Odin’s association with wolves, especially those that attend him, makes this a likely conclusion. This thesis, however, takes a different approach; that the Poetic Edda reinforces Hildolf’s status as Odin’s son by listing Thor and Hildolf’s names together. Moreover, a closer look at speech ascribed to Odin places Hildolf’s domain of Rathsey’s Sound within the river that separates Jotunheim and Asgard. Further analysis of cultural context also reveals symbolic and etymological parallels between Hildolf and the Ulfhednar, or Wolf-Coats, Norse warriors famously known for taking on the aspect of the wolf during battle. Through the synthesis of these disparate connections, this paper develops a clearer picture of Hildolf’s place in Norse mythology. Asgardian by birth, though not by station, Hildolf stands as the connection between gods and giants, between man and beast, and as Thor guides men to defend against enemies from without, Hildolf guides them to defend against the enemies from within. The conference presentation of this research stems from a longer work planned for submission to The Journal of Contemporary Heathen Thought.
Malagasy-English Bilingual Dictionary
Jackson Bell, Brigham Young University Humanities Since a military coup in 2009, Madagascar’s low standard of living, political instability, and weak economy have worsened. One way to improve the situation in Madagascar is to improve English skills, which are import in the tourism, exports, financial aid, and international academic collaboration sectors. For example, a knowledge of the English language will give Malagasy people access to 45 percent of the world’s scholarly research journals. I recently published a Malagasy-English bilingual dictionary which aimed to meet this need. However, the dictionary is somewhat impractical because it is limited to simple, rigid definitions. It is insufficient for a complete understanding of either Malagasy or English because real-life usage of words often deviates from basic definitions.
Understanding and Using Character Archetypes in Fiction
Michael Nielson, Dixie State University Humanities Character development is arguably the greatest driving force in fiction writing. But how does an author create a believable, complex character? Oftentimes, writers attempt to mimic characters they’ve encountered in literature. These characters repeated over time are generally known as character-specific archetypes; However, these archetypes delve deeper than writers simply copying other writers. The Oxford Dictionary of Literary Terms defines archetypes as a “symbol, theme, setting, or character-type that recurs in different times and places in myth, literature, folklore, dreams, and rituals so frequently or prominently as to suggest that it embodies some essential element of ‘universal’ human experience.” This paper will survey the effectiveness of writers consciously using character-specific archetypes—such as the hero, the sage, and the jester—drawing upon the expertise of Carl Jung, Joseph Campbell, and Victoria Schmidt with specific attention to J. R.R. Tolkien’s The Lord of The Rings series and Edgar Allan Poe’s short stories. Ultimately, I will suggest that the best way to create a believable, complex character is through the conscious use of archetypes, which allows the reader full immersion into the fictional work and fosters the suspension of disbelief.
Feminist and Mormon: Creating Identity Within Mormonism
Jeremy Lofthouse, University of Utah Humanities The identity of Mormon women has been sharply contested throughout the Latter-day Saint movement. Women in the nineteenth-century were targeted by critics of Mormonism as oppressed, even enslaved through polygamy. Following the termination of polygamy, Mormon women became hyper-domestic, following patterns of mainstream America. The identity of some Mormon women has made another dramatic transition in the last decade.
“The Sun Dance Opera”: A Centennial Performance
Meg Siner, Westminster College Humanities I would like to present my research on the 1913 Utah opera The Sun Dance Opera, written and directed by Lakota activist Zitkala-Sa. The opera, score, libretto are found in the L. Tom Perry Special Collections of the Harold B. Library at Brigham Young University. The opera premiered to rave reviews but has since fallen into obscurity.
The Ideal Woman
Jordan Bracken, Dixie State University Humanities Of the many problems facing woman today, one of the more serious is the unrealistic standard of beauty and behavior that women are expected to achieve, maintain, and accept as normative. However, I will show how select works of fiction, including Nathaniel Hawthorne’s The Birthmark and Edgar Allen Poe’s The Oval Portrait, exemplify the dangers of the male gaze; additionally, I will explicate non-fiction works such as Betty Friedan’s The Feminine Mystique and Simone de Beauvoir’s The Second Sex, which explore the severe consequences of attempting to force women to conform to a gendered societal paradigm. These works demonstrate the destructive characteristics to both men and women which can be easily overlooked by those who believe that a cultural standard, any cultural standard, represents a natural or necessary state of gendered behavior. When the image of the perfect feminine is idealized and internalized it both pressures women to alter their true identities and expects men to hold women to an unattainable behavioral and physical standard.
A Rhetorical Analysis of “The Adventures of Huckleberry Finn” Using Wayne Booth’s General Rules of Fiction
Benjamin Tullis, Utah Valley University Humanities Wayne Both has defined rhetoric as “the whole range of arts not only of persuasion but also of producing or reducing misunderstanding” (10). In The Rhetoric of Fiction, Wayne Booth also writes about four general rules that an author can use to enhance his or her writing and persuade readers. This rhetorical approach to fiction is not common. In addition, literary texts can be analyzed from a rhetorical perspective and many classical texts can be seen in a new way. My research uses Booth’s four general rules to dissect The Adventures of Huckleberry Finn. This book has been read and analyzed by millions of people since it was first published in 1884. My work finds a new correlation between Booth and Mark Twain. For example, Booth’s first general rule is that “…novels must be realistic” (23). Twain used his life experiences to create a world that, though it is imaginary, still rings true to the reader. Booth also states that, “all authors must be objective” (67), and “true art ignores the audience” (89). Twain was successful in following both of these rules because although he personally believed that that racism was wrong, he knew that other people, especially during his lifetime, did not share this same view. He used rhetoric indirectly in the story so that the reader can make up his or her own mind. Booth’s fourth rule states that authors should use “emotions, beliefs, and the reader’s objectivity” (119). Twain manipulates the emotions of his readers through the characters he created and the scenes he described. By carefully analyzing The Adventures of Huckleberry Finn in this new way using Booth’s four general rules, the modern reader will find a powerful rhetorically persuasive function in this classic text that many view primarily as children’s entertainment.
A time of Sadness: The Apostasy of Orson Hyde
Steven Hepworth, Weber State University Humanities Mormons and Missourians were at war with one another 1838. At the heart of the issue was the political and literal power of the Mormons in the state. The Mormon leader, Joseph Smith, claimed to have received revelations stating Missouri, and more particularly Jackson County, was to be the land of inheritance for Mormon Saints. Missourians feared a Mormon overtake of the State. During this same time the growing issue of slavery was the issue in Missouri. Mormons and Missourians found themselves on opposite ends of the slavery debate. These heated issues caused both Mormons and Missourians to persecute, harass, destroy, and fight one another. Orson Hyde changed the landscape of this conflict. On October 24, 1838 Orson Hyde signed a sworn affidavit declaring that Joseph Smith and the Mormon Church planned to overtake, possess, and control the State of Missouri. At the time, Orson was an Apostle, or leading member of the Mormon Church. He was well respected within the church and throughout the Missouri community. His testimony against the church and its leaders came as a shock to many. He showed no sign of discontentment previous to his signing a sworn affidavit against Joseph Smith and the Mormon Church. Why would a leading member of the Church of Jesus Christ of Latter day saints testify against his religion, which he still held dear, and his friends? Orson Hyde indicted Mormon leaders of treason to save the lives of his family, to placate non-Mormon neighbors, and because of a failed mind due to illness. I will explore the involvement of Hyde in the Mormon Missouri war, what led him to testify against the Mormon Church, and the consequences resulted from his testimony.
Chirstmas Lists
Carson Bennett, Brigham Young University Humanities A short story based on my grandfather’s experiences in the Battle of the Bulge.
Self-Regulatory Capacity and Vocabulary Acquistion
Jacob Newman, Brigham Young University Humanities In ‘A New Approach to Assessing Strategic Learning: The Case of Self-Regulation in Vocabulary Acquisition’ Tseng et al. (2006) discuss their design of an instrument that measures learners’ self-regulatory capacity rather than use of specific learning strategies. Learning strategies include a variety of behaviors or activities that a learner does to help them during the learning process. This instrument (SRCvoc), designed as a questionnaire, aims to help learners discover their self-regulatory capacity and then apply personalized learning strategies that are beneficial to their own vocabulary learning. Vocabulary learning is essential in developing language skills. According to their research, SRCvoc ‘can serve as a diagnostic measure to identify and understand learners’ strengths and weaknesses in terms of the five areas of self-regulation in the area of (English vocabulary) learning’ (Tseng et al. 2006: 96). The goals of the study, per Tseng et al. (2006) were (a) to create an instrument that ‘measured learner trait of self-regulatory capacity rather than survey specific behavioral habits’ (b) to create ‘an instrument based on a theoretical construct.’ SRCvoc is based on self-regulatory strategy research from the area of educational psychology, with facets including commitment, metacognitive, satiation, emotion, and environmental control and (c) to design an instrument “in one particular learning domain only, that is vocabulary learning.” We replicated the administration of SRCvoc to verify that it is of value beyond the original context. With assistance from Dr. Neil Anderson and Dr. James Hartshorn, I administered the questionnaire through a Qualtrics survey to learners in the academic program at Brigham Young University’s English Language Center (ELC) to replicate the original administration of SRCvoc. I created and distributed self-regulatory profiles from the results of SRCvoc to the students. We examined the validity and reliability of SRCvoc through statistical analysis and learned more about training self-regulated learners.
The Greek Male Nude in Art: Heroic Herakles and Ephebic Apollo
Lauren Harding, Utah Valley University Humanities From the Classical world, the male nude has come to be one of the most recognizable symbols for Greek civilization. This nude is a protean and multifaceted entity that took on different meanings as Hellenic society progressed. Two binary forms of these nudes recur as archetypes, which in this paper are identified as (1) the Heroic/Athletic nude and (2) the Ephebic/Mythological nude. Within the new discursive framework of these two physiques, it is necessary to incorporate the recent research that has been produced after the large feminist and gender studies awakening that has swept through the Classical scholarly world. The field has been wonderfully enriched with this research, and as such a re-evaluation of the male form is in order. This investigation will reveal new and fascinating aspects of Greek sculpture that previously have not been studied, and will illustrate how the distinctions between the two nudes are more easily seen, or, in certain cases, how these distinctions are blurred. Through better understanding this complicated, omni-sexual world of Classical Greece, we will enrich the understanding of our own culture, which is more nuanced than the hetero-normative standard that many believe exists.
Through the Dark Ages and into the Light How Did Christianity and the Catholic Church Influence the English Language?
Ward Symes, Dixie State University Humanities Some people think a heavy blanket of intellectual darkness was thrown over Europe after the fall of the Roman Empire and not lifted until the Renaissance. Were the Dark Ages a time of total illiteracy when learning and education ceased to exist? Did the Dark Ages only end with the emergence of great milestones like the invention of Gutenberg’s movable type printing press and Martin Luther protesting the Catholic Church?
The Ward
Bronson Beatty, Dixie State University0 Humanities If we are the targets of bullying and slander, we have the right to defend ourselves and our good name. But how thick is the line separating self-defense from striking back or taking vengeance? This short story, inspired in part by Poe’s classic tale “The Cask of Amontillado,” is historical fiction set in the era of Renaissance Italy and its warring noble families. Fabiano, our protagonist, is the son of fabulously wealthy merchants. He is a respected pillar of his community and hosts tri-annual masques which are the toast of southern Italy. However, the son of a powerful northern family arrives uninvited and reveals that Fabiano was a ward to his family in their youth, humiliating him in front of his guests. Fabiano’s tormentor visits every masque for over a year, insulting Fabiano further and gradually turning him into a laughingstock. Fabiano becomes obsessed with restoring his honor and with defeating his tormentor, but his schemes may carry too far. How far can we go trying to right wrongs committed against us? Is it always right to do so?
Language learning success
Andrew Gibson, University of Utah Humanities In many languages we have words that don’t have a one-to-one correlation, for instance, ‘Wednesday’ sounds more like ‘wensday’. Although writing systems can be consistent, some syllables are pronounced differently in some contexts. The point is; that words have different sounds from their written form. I theorized this as ‘word deceptive.’ These words can often deceive us when spelled differently from the way they sound. This can be difficult for others learning a language. But to aid in this difficulty is with word deceptive strategies: 1.Make the unfamiliar familiar 2. Associate visuals to the word to increase word retention 3. Use words in interpersonal experiences.
No! No! No! to GO! GO! GO!
Daniel Howell, Utah Valley University Humanities Over the years research has proven again and again the important role that sexual satisfaction plays in marriage and relationships. Researchers have identified emotional safety, sexual frequency, sexual communication, sexual communion, sexual ability, and sexual arousal as elements that are important elements that influence sexual satisfaction. The aim of this study is to identify which of these elements are more effective in predicting sexual satisfaction and which ones are not. Using as a sample of 597 participants which primarily represented a white, LDS, middle class to upper class population. One of the questions that had to be asked was, are there differences between men and women in the variables and what are they? Another question that had to be asked was where do these factors rank in importance? Which factors are the most important? The study showed that when emotional safety, sexual frequency, sexual communication, sexual ability were increased, sexual satisfaction was increased. Sexual communion had a positive influence on sexual satisfaction as well. As long as the other partner focused on that individual. Another significant finding is that in sexual arousal played a more significant role in satisfaction for women than it did men. The goal of this study is to publish the results in scholarly journals and to implement the findings into the family life education program.
Brown v. Board: The Racial Meridian
Hayden Smith, University of Utah Humanities While serving as a full-time volunteer missionary for the Church of Jesus Christ of Latter-day Saints from 2010-2012, I gained a new perspective of discrimination and racism in contemporary America. My mission was located in West Texas and East New Mexico. While living in this region, I came into contact with a much different setting then I had experienced growing up in Salt Lake City, Utah. I learned Spanish and worked very closely with the Latino and also the African American minority populations. This was compelling as I learned a great deal about the types of discrimination which minorities experienced in the United States. In Lubbock, TX in particular, I saw a very segregated community, as the city was still divided upon racial lines.
Reception of the Enlightenment in Portugal
Mary Ellison Barlow, Brigham Young University Humanities In the 15th and 16th centuries of the Portuguese golden age was followed by a period of decline in the 17th and 18th centuries. For a majority of Western Europe, this latter period heralded the age of Enlightenment and brought with it a cultural movement of reason and individualism that changed the history of the world. There is a vacuity surrounding Portugal in respect to the role and level of influence it had in the movement of the Enlightenment. The purpose of this study is to discover how Portugal received enlightened European thinking and how the enlightened world saw Portugal. This study will include the affect the Lisbon Earthquake of 1755 influenced Enlightenment thinking, foreign and domestic. We will also analyze the reception of ones such as Mozart and Voltaire and their corresponding works, and how these ideas influenced Portuguese society.
Economic Theory and the Holocaust
Spencer Yamada, Brigham Young University Humanities This project is designed to use the actions of the US military in response to American civilians advocating for direct military intervention in the holocaust as a case study to apply the economic Theory of Disruption. The complex situation during the war, involving Anti-Semitism, technical challenges, political motives and military bureaucracy has traditionally been impossible to untangle. Existing theories of management and economics were applied using their data-proven ability to understand human behavior. Clayton Christensen of Harvard Business School developed the Theory of Disruption to describe the process by which companies are able to innovate, grow, and conversely stall and fail. Christensen found that large companies that had traditionally been innovative and successful had become so by bringing new products to new markets that had been previously ignored. Christensen noted that the world’s most successful corporations had changed dramatically overtime by initiating self-disruption and changing focus from what has traditionally been successful for them to smaller new markets and new products. The Allied purpose during the Second World War was centered on one key policy decided at the Bermuda Conference in 1943, which was to defeat Nazi Germany. Around the same time groups of both Gentile and Jews began clamoring for a new strategy involving the bombing of Auschwitz as reports of the camps reached the American public. The American Military did not produce the product this small market demanded, acting in the same way a large corporation would that failed to initiate the process of disruption and took no action against any holocaust targets. Christensen’s Theory of Disruption explains of this phenomenon as a function of economic forces and organizational behavior.
Social critique through a close reading of Jorge Ibargüengoitia’s play Llegó Margo
Nicholas Sheets, Brigham Young University Humanities This honors’ thesis analyzes a lesser-known drama by Jorge Ibargüengoitia, Llegó Margo (1956), by approaching historic social critiques in Mexico through dialogue, plot, and character development. Readers will approach this play through various elements of Marxist and historical criticism. Through this, higher significance to social themes develops when considered in the historical context of the semicentennial celebration of Mexico’s revolution of 1910. The play then falls into a broader national dialogue of Mexico’s social structures post-revolution. Research for this thesis includes the Ibargüengoitia papers at the Firestone Library, an interview with Joy Laville (the author’s widow), and travels to various locations in Mexico, ultimately resulting in the thesis’ higher awareness to historic social themes and a richness of Mexican ideals. Ibargüengoitia offers a critique of & for his own middle-class audience, calling attention to its hypocritical treatment of the poor as well as a hyper awareness to social traditions which ultimately maintain an unsympathetic class structure in Mexico.
FInding Hemingway
Paden Carlson, Utah State University Humanities Historically, many artists have struggled with mental illness; they use their art as a way to cope with, and explore, their troubled lives. Writers, in particular, often seem to turn to writing when their situations seem empty or their lives appear to be in ruins. Sylvia Plath, Virginia Woolf, Anne Sexton, Mark Twain, and F. Scott Fitzgerald all suffered from depression. Some of their best work originated from their pain. Ernest Hemingway also suffered from depression, though it never manifested itself in his work. Part of my project is to read the letters he wrote to his doctors to see if he reveals his struggles through his correspondence in a way that he doesn’t in his fiction. I’d like to read these letters with my own depressive struggles in mind and think about the relationship between art and depression, thereby coming to better understand my own need to create.
Development of a Resin-Free Protein Purification Technique Utilizing Unique Biochemical Properties of the E. coli SSB Protein
Mark Soffe, Utah State University Life Sciences SSBs are DNA binding proteins that are essential components of cells and play key roles in DNA replication, repair, and recombination. Here we utilize two biochemical properties associated with the E. coli SSB protein to develop a novel procedure to purify proteins using a resin-free strategy. 1. E. coli SSB binds to single stranded DNA (ssDNA) with extremely high affinity (K = 1013 M-1), indicating very tight binding. 2. It is also a unique protein with respect to its purification – it is possible to obtain greater than 95% pure SSB from the total cell lysate without using any sort of column or resin, utilizing polyethyleneimine (PEI) and ammonium sulfate precipitation. Our design uses SSB as an affinity/solubility tag to enhance the solubility and expression of difficult-to-purify proteins, and allows for the simple, resin-free purification using PEI and ammonium sulfate precipitation. There also may be a possibility to co-express protein dimers and possibly tetramers using this method. Constructs have been made that include the SSB gene, along with the ability to fuse any gene of interest, as well as a TEV Protease cleavage sequence allowing for proteolytic cleavage after gene expression. Two genes of interest have been cloned in thus far—TEV protease and Rad51. In this proposal I outline experiments to develop this strategy further and test our proof of principle concept and its application to a broader set of target proteins.
Urban Noise as a Stressor in Side-Blotched Lizards
Marilize Van der Walt, Utah State University Life Sciences Urbanization is accompanied by a lot of changes to the landscape that have the potential to affect the native species inhabiting the area. If animals are chronically exposed to these anthropogenic disturbances and are unable to acclimatize, changes in circulating glucocorticoid hormones may cause adverse effects to the animal’s health, such as an impairment in innate immune activity. One such disturbance is human-induced noise. Using the side-blotched lizard, Uta stansburiana, because of their localized habitat and inability to escape such disturbances within the urban environment, we looked at the field and lab components of noise as a stressor. We first measured decibel levels in urban and rural field sites in their natural habitats in St. George, Utah, and conducted a lab study exposing U. stansburiana to either a synthesized urban sound recording or no sound recording (control) for nine days. We collected blood samples and measured circulating corticosterone and testosterone concentrations and bactericidal ability to determine if there are endocrine and immune alterations in response to increased noise decibels. Our results show that lizards exposed to urban noise experienced increased corticosterone levels indicative of stress. Furthermore, bactericidal ability was indirectly affected by noise through significant correlations between body condition and corticosterone and testosterone concentrations. These results indicate that an increase in ambient decibel levels acts as a stressor to animals in urban areas. By dissecting out an individual component of a complex stressor we can better understand the effects of urbanization as a whole.
Plant-Soil Feedback of Native and Exotic Species in the Inter-Mountain West
Molly Van Engelenhoven, Utah State University Life Sciences For thousands of years farmers have known that soil organisms can destroy crops but only in the past ten years have researchers begun to understand that complex interactions among soil organisms and plants can determine plant diversity and productivity. Much of the research in this young field of study has come from qualitative greenhouse experiments and separately from simple mathematical models. Here, we report findings from a six-year plant-soil feedback (PSF) field experiment that assessed the effects of soil organisms on the growth of twelve plant species common to the intermountain west. Initially, twelve native and exotic species were randomly assigned to 1550 experimental plots in a field. After four years of growth, the plants were killed. Next, the same plant species were randomly assigned to the 1550 plots again to test how each plant species responded to the soil microbial communities ‘cultivated’ by each other plant species. Two years later plant growth responses to different soil types were measured. Most plants showed large growth differences on different soil types. For example, Bromus tectorum grew twice as much on Pseudoroegneria spicata-cultivated soils than on self-cultivated soils. When the plant growth responses observed in this experiment were used in a mathematical model, PSFs were found to play a critical role in determining which plants were likely to be dominant in a community and how productive these different plant communities would be. In summary, this large-scale experiment provides strong evidence for the role of PSFs in plant productivity and diversity. Results have implications for natural methods of effective native plant restoration, biofuel production and weed control.
Long-term evaluation of Leafy Spurge biological control in Richmond, Utah
Jacob Anderson, Utah Sate University Life Sciences Leafy spurge (LS) is an aggressive Eurasian forb that has been successfully reduced in many areas in western North America through the biological control releases of flea beetles. Long term studies of this phenomenon are sparse. Three flea beetle species were released in the mid-1990s at a site dominated by LS in Richmond, Utah. This study assessed the long term effects of LS biocontrol on an ecological community at this site by addressing five questions: (1) Is LS abundance significantly lower now than in the 1990s? (2) What plant species are replacing LS and are they native or non-native? (3) Have the flea beetle populations persisted since their initial release? (4) What part does soil type play in which flea beetle species now dominate at the site? (5) In response to their unexpected presence, what role may long-horned beetles contribute to the long-term reduction of LS? It was found that LS abundance has significantly decreased from the 1990s; the dominant plant species are those of non-native grasses; flea beetles have persisted in significantly smaller numbers, with Aphthona lacertosa being the most abundant; and long-horned beetles appear to play a significant role in the reduction of sexual success of LS. The results of this project have implications for land managers when considering the vegetative response to LS biological control and the importance of long-horned beetles for long-term in managed, LS-reduced habitats.
Advancement of Petroleum Diesel Alternatives Utilizing a Multifaceted and Interdepartmental Approach
Michael Morgan, Utah State University Life Sciences The advancement of biologically derived alternatives to petroleum diesel fuel requires a multifaceted approach. At Utah State University we use an interdisciplinary team including the Colleges of Engineering, Agriculture & Applied Sciences, and Science in conjunction with industry partners to drive innovation in improving the science behind petroleum diesel alternatives. With increasing petroleum use, depleting reserves, increasing emissions standards, and other factors, there is need for petroleum diesel alternatives that are cost effective, offer improvement, and perform similarly to petroleum diesel. Our team has focused on the use of oleaginous microbes utilizing low value effluent and waste sources including sugars and CO2 to create biofuels. We have focused on a yeast, Cryptococcus curvatus, and a microalgae, Nannochloropsis salina which have shown high yields of fuel per cell mass. Using these microbes we have utilized USU’s own direct trans-esterification reaction to create sufficient quantities of biodiesel for engine performance and emissions testing, including a subset of ASTM tests characterizing the fuels from each organism. Our initial engine testing used petroleum diesel as a baseline in conjunction with commercial soybean biodiesel to establish the quality of our microbially derived biodiesel. Testing in stationary diesel engines and on the Bonneville Salt Flats has proven our microbial fuels perform similarly to soybean biodiesel and comparably to petroleum diesel. To further improve biological diesel replacements we have begun working to create green diesel, hydrocarbons from a biological source, using a novel method of hydrothermal liquefaction. Preliminary results of those tests are presented here. Through a multifaceted and interdisciplinary approach USU is successfully improving petroleum diesel alternatives from microbial sources including characterization of the properties of these fuels and is working to create the fuels at the scale necessary for exhaustive engine performance and emissions testing including ASTM testing of all important fuel properties.
Characterizing breast cancer cell lines using principal component analysis of high- frequency ultrasonic spectra
Laurel Thompson, Utah Valley University Life Sciences Breast cancer is divided into subtypes which are defined by their proteomics, histology, and genetic expression profile. Current methods, therefore, are aimed at testing these, and include DNA microarrays, immunohistochemical staining, and proteomic analysis. These methods are effective classifiers, but are not easily transferable to real-time clinical applications, such as the determination of cancerous status during operation or when taking a biopsy. The determination of molecular subtype by other means would be a significant advancement in cancer detection and treatment. We have made some preliminary studies that suggest high-frequency ultrasound may be sensitive to variations among the cancer subtypes as manifest in cell cultures through their cytoskeletal protein structure, which has a distinct spectral signature. The object of this study was to explore the basis for this variation through a combination of experimental and theoretical analysis. We used first-principal modeling methods and compared the model spectra generated from these to spectra obtained in the cell culture lab. Variations in bulk modulus, cell position and size were modeled and combined with experimental spectra in principal component analysis (PCA), and the Euclidean distances between each principal component of the experimental were found as they relate to the theoretical principal components. A graphical method similar to heat maps used for gene expression profiling was then developed to display the relative distances (similarities) between spectra. The program was tested by comparing experimental spectra of three breast cancer cell lines to model spectra. The results indicate the properties and thus molecular subtypes of breast cancer cells could potentially be determined by comparing their measured spectra to model spectra using a feature classification program such as PCA and that these classifying features can be displayed in a convenient graphical representation according to their spectral similarities.
Physiological effects of habitat disturbance in the wandering gartersnake (Thamnophis elegans)
Austin Spence, Utah State University Life Sciences This study investigated the physiological effects of localized habitat disturbances around two well-studied hibernacula of the wandering gartersnake Thamnophis elegans. After three years of monitoring snake morphology and physiology at several hibernacula, several disturbance events occurred, including log clearing, stream bank disturbance, and vegetation removal. Individuals from three populations, two with disturbed hibernacula and one control population with no disturbance, were collected during the spring emergence immediately following the disturbance. Blood samples were collected upon capture and following a uniform stressor to measure baseline and post-stress physiological conditions. The samples were analyzed using a radioimmunoassay to measure corticosterone levels and a bacterial killing assay to measure innate immunocompetence. Baseline and post-stress corticosterone levels were higher in both populations with disturbance events compared to the control population. The bacterial killing ability of the site with the most anthropogenic activity was lower than the control site, indicating immunocompromise. Data are currently being analyzed to assess differences within the same populations between years with and without disturbance events. Pre-disturbance data are a rare and useful commodity and allow us to facilitate a better understanding of the various effects of anthropogenic change on natural populations. This study was funded through the Undergraduate Research and Creative Opportunity Grant from Utah State University.
Copper Resistant Phenotype in MDM35-deletion Saccharomyces cerevisiae
Jacob Bassett, Utah Valley University Life Sciences Many discoveries regarding the complex interplay between biological pathways within a cell begin with attempts to link new observations to scrupulously studied cellular mechanisms. Our lab is studying the soluble inter-mitochondrial space protein mdm35, which has been observed to facilitate the function of ups1 & 2 proteins, as they in turn regulate the mitochondria’s phosphatidic acid metabolism. In addition to this phenotype, our lab has observed a copper resistance at 0.17% on YPD plate and 0.14% in liquid cultures of S. cerevisiae lacking the mdm35 protein, when compared to the Wild Type strain. Our investigation measures the levels of expression in the cup1 and ctr1 promoters in an attempt to link this observation to a potential mechanism contributing to this resistance.
Genomic analysis of six Paenibacillus larvae bacteriophages
Bryan D Merrill, Brigham Young University Life Sciences The spore-forming bacteria Paenibacillus larvae causes American Foulbrood (AFB), a highly contagious disease that is lethal in honey bee larvae. P. larvae is the most serious pathogen affecting honey bees. Its increasing antibiotic resistance has led to more research in characterizing bacteriophages which infect and destroy P. larvae. Of the 13 P. larvae phages that have been described in publications, six have been fully sequenced and are currently available for genomic analysis. To understand how P. larvae phages are related to each other and to other phages, the computer program Phamerator was used to analyze more than 100 phage genomes and group phage genes into “phams” based on similarity. Through analysis of grouped phage genes (structural proteins, terminases, recombinases, etc.) we can better understand in vivo replication strategies and evolutionary history of these P. larvae phages.
Ethanol inhibits gaba neurons in the ventral tegmental area and dopamine release in the nucleus accumbens via presynaptic alpha-6 nicotinic receptors on gaba terminals
Taylor Woodward, Brigham Young University Life Sciences The prevailing view is that enhancement of dopamine (DA) transmission in the mesocorticolimbic system, consisting of DA neurons in the ventral tegmental area (VTA) that innervate the nucleus accumbens (NAc), underlies the rewarding properties of alcohol and nicotine (NIC). Dopamine neurotransmission is regulated by inhibitory VTA GABA neurons. We have shown previously that VTA GABA neurons are excited by low-dose ethanol, but inhibited by moderate to high-dose ethanol. The aim of this study was to evaluate the role of []6 nicotinic cholinergic receptors (nAChRs) in ethanol effects on VTA GABA neurons as well as DA release in the NAc. In electrophysiology studies, superfusion of ethanol enhanced the frequency, but not amplitude, of mIPSCs recorded in acutely dissociated VTA GABA neurons from GAD GFP mice. The []6 nAChR antagonist []-conotoxin P1A did not affect mIPSCs, but prevented the ethanol (30 mM)-induced increase in mIPSC frequency. While microdialysis studies show that ethanol enhances DA release in the NAc, we and others have found that ethanol decreases DA release at terminals using fast scan cyclic voltammetry (FSCV). We have reported that ethanol inhibition of DA release at terminals in the NAc of ethanol-naïve animals is mediated by GABA. Using FSCV in the slice preparation, ethanol inhibited DA release in the NAc. Superfusion of the []6 nAChR antagonist []-conotoxin MII did not affect DA release, but prevented ethanol inhibition of DA release. Taken together, these findings suggest that ethanol enhancement of GABA inhibition of VTA GABA neurons is mediated by []6 nAChRs located on GABA terminals to other VTA neurons, affecting DA release in the NAc. Results from this study could provide a pharmacologic rationale for considering drugs that act selectively on nAChRs as therapeutic agents for the treatment of alcohol dependence and alcohol and NIC co-dependence.
Biodegradation of Hydrocarbons by Great Salt Lake Microorganisms
Camrin Rivera, Westminster College Life Sciences The Great Salt Lake (GSL) of Utah is home to organisms that are able to survive and reproduce in waters that can have a salt concentration of over 30%. Oil Jetty is located on the saltier north arm of the GSL and is the location of a natural hydrocarbon seep that flows into the lake. Previous work showed that organisms capable of degrading hydrocarbons can be cultured from this area (Ward & Brcok, 1978). It has also been shown that the salt concentration has an inverse relationship with these organisms’ ability to degrade hydrocarbons (Ward & Brock, 1978). This previous study gives insight of the hydrocarbon degrading capabilities of microorganisms in the GSL; however, the identification and characterization of individual hydrocarbon-degrading microorganisms has yet to be completed. The purpose of this study is to identify GSL microorganisms capable of degrading hydrocarbons under high salinities by sequencing of the 16s rRNA gene. Future studies will include the characterization of these microorganisms in order to find isolates whose hydrocarbon degrading abilities could be utilized for bioremediation and biotechnologies.
The effect of anxiolytic mediation administration on interval timing distracters
Chance Christensen, Utah State University Life Sciences Affective disorders such as depression, phobias, schizophrenia, and post-traumatic stress disorder impair the ability to time in the seconds-to-minutes range, i.e., interval timing. According to the Relative Time-Sharing (RTS) model, presentation of task-irrelevant distracters during a timing task results in a delay in responding suggesting a failure to maintain subjective time in working memory, possibly due to attentional and working memory resources being diverted away from timing. Given that some anxiolytic medications have beneficial effects on attention and working memory, e.g., decreasing emotional response to negative events, we hypothesized that they would result in a decreased effect of distracters on the timekeeping abilities. We investigated the effect of acute administration of anxiolytic medication when anxiety-inducing task-irrelevant distracters were presented during an interval timing task, using methods similar to Matthews et. al. (2012) Frontiers in Integrative Neuroscience 6(111): 1-12. Results are discussed in relation to the brain circuits involved in RTS of resources, and the pharmacological management of affective disorders.
Frog Behavior May Protect Against Potentially Lethal Chytriodiomycosis
Hailey Shepherd, Dixie State University Life Sciences Previous studies of canyon tree frogs (Hyla arenicolor) in Zion National Park have shown that some populations test positive for a dangerous fungus in the Chytridiomycosis family. This fungus has been linked to large population losses worldwide in many keystone amphibian species, but appears to have no effect on populations of H. arenicolor. Since Chytrid fungal growth is inhibited at high temperatures, we hypothesized the frogs are able to rid themselves of the fungus because they bask in the sun. During the summer of 2013 we swabbed frogs in multiple slot canyons to test for the presence of the fungus. We also recorded skin temperatures of the frogs we swabbed. Skin temperatures were as high as 38 C°, which is above the previously established lethal threshold of 28 C° for Chytridiomycosis. Our data support the idea that these frogs may be able to rid themselves of infection by allowing skin temperatures to raise enough to become intolerable for this fungus. This is the first known evidence of wild amphibian populations showing a behavior that may clear the infection. If further research supports these findings, it could lead to more effective allocation of limited conservation resources.
Do Canyon Tree Frogs in Zion National Park Possess a Mechanism to Defend Against Chytrid?
Chelsea Moody, Dixie State University Life Sciences A relatively new pathogen, chytrid fungus, has been a major cause of decline in amphibians worldwide. This pathogen has been found on Canyon Tree Frogs in Zion National Park. Earlier data suggested that chytrid did not affect populations of Canyon Tree Frogs in Zion National Park. We predicted that since the populations weren’t impacted by the presence of the fungus, there must exist a mechanism by which the frogs rid themselves of the infective agent. To test this prediction, we captured and swabbed ten frogs in each of nine canyons in Zion National Park during the summer of 2013. Swabs were sent to the San Diego Zoo to be analyzed for the presence of chytrid DNA. Chytrid infection rates and population sizes were compared with data from previous years. As expected, the data showed that two of the three infected canyons experienced declines in the percentage of frogs testing positive for the fungus. Thus, these frogs must possess a mechanism to survive this pathogen, whereas most amphibians do not. Our results may prove helpful to other biologists seeking to understand how amphibians can survive this pathogen.
Attachment reduction of invasive species with submersible substrates
Eric Swenson, Dixie State University Life Sciences Underwater invasive species such as mussels and algae attach to submersible substrates that are advantageous for growth, and often include recreational boats that are frequently moved from one waterway to another. This can lead to rapid and uncontrolled spread of the invasive species. Through the use of newly developed ionic surfactants, growth and attachment of species on submersible surfaces can be inhibited. New surfactants have been incorporated into the final coating of typical product sealants to prevent the attachment of various marine organisms. By integrating the surfactant into the coating, an increase in the usefulness and longevity of effective inhibition is expected, as compared to commercially available alkali anti-fowling agents that are applied after the sealing process. It is hypothesized that the adherence properties possessed by marine organisms will be compromised by the introduction of ionic surfactants through changes in the chemical makeup of the exposed surfaces. The ultimate goal is to develop a substrate surface in which there is no affinity for attachment and/or causes fatality to the organism. If the desired outcome is reached, the potential benefits that ensue could create drastic improvements in areas of invasive species prevention and management in the desert southwest fresh-water lakes. Additionally, extension of the surfactant application to metal surfaces could have significant impacts on efficiency in water treatment systems, as well as cargo shipping in saltwater environments.
Waterfowl Population Trends, Pariette Wetlands, Utah 1980-2010
David Baird, Utah Sate University Life Sciences We obtained from the Bureau of Land Management (BLM) 30 years of monthly waterfowl population surveys completed at Pariette Wetlands in the Uintah Basin, Utah between 1980 and 2010. The Pariette Wetlands are the largest wetlands managed by the BLM within the lower-48 states and are comprised of 4,033 acres of land. Pariette Wetlands is surrounded by about 6,000 square miles of land where oil and gas production is the major activity. This waterfowl refuge is a significant location for migrating waterfowl species within the Pacific Flyway and provides important summer habitat for resident waterfowl. Our objectives were to determine what the trends were for waterfowl population abundance, occupancy, and species richness.
Utah Valley University/Huntsman Cancer Institute Collaborative Breast Cancer Study: High-Frequency Ultrasound for Margin Assessments
J Andrew Chappell, Utah Valley University Life Sciences In a joint effort with Huntsman Cancer Institute at the University of Utah, students from Utah Valley University are using high-frequency (HF) ultrasound to test the pathology of surgical margins from breast cancer conservation surgery. The method, developed by Dr. Timothy E. Doyle, provided significant results in a NIH-funded 2010 feasibility study. The results of the study indicated that peak density, the number of peaks and valleys in the HF ultrasonic spectrum, correlates to breast tissue pathology. This technology would allow surgeons to test – in the operating room – whether a surgical margin was clean or if cancer still remained in the margin. This advancement would decrease the amount of return surgical visits a patient must undergo, reduce costs for patients and hospitals, reduce breast cancer recurrence rates, and ultimately increase the survival rate of patients with breast cancer. During the ultrasonic testing, the students work in a team of four in a room outside of the surgical suite. Specimens are brought in by the surgeons’ team and tested immediately following resection. The margins are approximately 3x20x20 mm in size, and are oriented using a small staple inserted by the surgeon in one corner and a stitch on one side. The margin is tested at specific locations depending on the size of the margin and then sent to pathology for analyses. Pathological results and HF ultrasound results will be compared for correlation at the end of the study, which is expected to last about one year. The study will include approximately 80 patients, 360 tissue samples, 1400 tested locations, and 4,300 data points. The goal of the study is to evaluate the accuracy of the method in determining margin pathology. If successful, the method will be moved into clinical trial.
Standing on the shoulders of Woodrats: Adaptive Evolution in Desert Iguanas
Albert Pope, Utah Valley University Life Sciences Few animals are capable of using the creosote plant, Larrea tridentate, as food because of a high level of toxic secondary compounds. Some exceptions to this rule are Neotoma lepida and Dipsosaurus dorsalis which are both capable of sustaining themselves on this desert bush. In 2013, Magnanou et al helped identify heightened transcription of genes correlated with digestion of creosote in N. lepida. Building upon their findings, we explore whether the genes for digesting creosote are under an elevated evolutionary rate for D. dorsalis. We have obtained transcriptomes from whole blood of four Iguaninae species: Ctenosaura pectinata, D. dorsalis, Sauromalus ater, Cyclura lewisi yielding an average of 4 GB of DNA sequence data (~51,000,000 fragments) each. Using Velvet in Sequencher we assembled these data, recovering greater than 6000 unique RNA transcripts per transcriptome. We search through the contigs to identify genes in Iguaninae transcriptomes that are homologous to those showing differential expression in Lepida. Using BLAST, we retrieve homologous genes from the public NCBI database of Anolis carolinensis and other reptiles. Lastly we construct phylogenetic trees of each gene and investigate the rate of change along each reptile lineage.
The effect of residue 21 on drug resistance of the A/M2 proton channel of the influenza virus
Cameron Haas, Brigham Young University Life Sciences The influenza A virus contains a proton-selective ion channel, A/M2, through which acidification of the cell is induced. A/M2 is a homotetramer (consists of four identical helices) consisting of 97 residues and activated by low pH levels. Mutations in the amino acid sequences may induce resistance to channel inhibiting drugs. It is believed that residues 26, 27, 30, 31, and 34 are the major contributors of drug resistance, but other nearby residues may prove important as well. The A/California/04/2009 version of the influenza virus is sensitive to the drug AK-11, while its M2 channel is not. The A/Udorn/307/1972 with the S31N mutation M2 channel has been shown to have reduced sensitivity to amantadine compared to its wild type. While both contain a D (aspartic acid) at residue 21, A/Puerto Rico/8/1934 has a mutation from D to G (glycine). The A/Puerto Rico/8/1934 virus A/M2 contains mutations S31N and V27T and has shown sensitivity to the AK-11 drug, but the mechanism of inhibition of the A/M2 channel has not been verified. In these experiments we will be identifying sensitivity to AK11 of A/Udorn/307/1972 with the S31N mutation as well as inducing double mutations with S31N at residues 27 and 21 in the A/M2 from the virus and measure sensitivity by electrophysiological recordings in oocytes of Xenopus laevis. By doing so we may identify the role of these residues in drug resistance and the effects of these amino acid mutations, while verifying the A/M2 channel as the mechanism of acidification inhibition and drug sensitivity. We hypothesize that either D21G, V27T or both mutations causes drug sensitivity in M2 S31N, explaining the sensitivity of A/Puerto Rico/8/1934 to AK-11.
Physiologically Relevant Oxy-Radical Formation of Neuromelanin by Photostimulation: Effects of Iron and Calcium
Whitney Badal, Brigham Young University Life Sciences Light therapy has been utilized to treat alcoholism and opiate-dependent rats as well as ameliorating symptoms of Parkinson’s disease. As both addiction and Parkinson’s Disease (PD) are often associated with decreased dopamine transmission in the striatum, it is likely that light therapy is able to increase dopamine release. A similar technique called near-infrared light treatment has also been shown to be effective in mice in restoring the function of dopamine cells in the substantia nigra pars compacta (an area associated with PD). A possible explanation for this is that light catalyzes the formation of neuromelanin. It is likely that neuromelanin is a neuroprotective cellular agent that is able to reduce damage caused by reactive oxygen species. Using UV-IR spectrophotometry, we show that in the presence of photostimulation, dopamine (0.3-30 uM) oxidizes and polymerizes into neuromelanin. Since hydrogen peroxide catalyzes this formation of neuromelanin, it is likely that this is a radical-polymerization reaction, suggesting that neuromelanin may be a radical scavenger. Additionally, the presence of the selective iron chelator desferrioxamine, the calcium chelator EGTA, or lack of calcium in the artificial cerebral spinal fluid markedly reduces the formation of neuromelanin. Using fast scan cyclic voltammetry in mouse horizontal and/or coronal brain slices, dopamine release in the nucleus accumbens core was enhanced by light exposure, in particular UV and short-wavelength visible light. These findings indicate that both iron and calcium are necessary for melanization in neural tissues and that light-induced melanization enhances dopamine release, suggesting a physiological role for melanization in synaptic transmission.
Insights into the evolution of wings in Insects: Molecular Phylogenetics of Baetidae (Ephemeroptera)
Chase Barker, Utah Valley University Life Sciences Central Research Question: Phylogenetic relationships of mayflies are still not very well known, however molecular and morphological data have begun to shed light on the relationships of these insects (Ogden et al. 2009). Our central question is to elucidate the phylogenetic relationships within the mayfly family Baetidae.