2013 Abstracts

S. Geneva Balin, Weber State University Anthropology In the past, mythology served as a guide to how life should be lived and provided a context for the condition in which people found themselves. As the sacred gave way to the secular, mythology lost its power to influence and guide the people as it did in the past. Rather than myths, people turned to literature for guidance. These stories have been told and retold through different eras in history. They have also been shared in contemporary forms such as books and movies which now reach a large audience. This paper will examine stories of social outcasts in an interpretive exploration into culture through the lens of literature. The multiple versions “The Phantom of the Opera” and “The Hunchback of Notre Dame” reflect the cultures in which they were produced as well as the contemporary cultures that love them. A basis for comparison will be established by first recounting, in summary, the plotline for each of the novels. Then theories relevant to exploring meaning will be approached, notably those of Claude Levi-Strauss, Émile Durkheim, Dame Mary Douglas and Carl Jung. From here, variations of the stories and the history surrounding them can be examined to discover potential social meaning and show the importance of stories as a way to bind a culture together.

James Gardner, Utah State University Anthropology Distinct perceptions on healthcare reform exist in every part of a society. This paper examines the volatile healthcare system of Nicaragua and the perceptions of healthcare reform among Nicaraguan medical professionals. Data were gathered through ethnographic field methods including participant observation, informal interviewing, and open-ended questions. The informants were selected from the medical personnel of the E.R. in the Hospital Amistad Japón-Nicaragua in Granada, Nicaragua. First, a framework of the history of Nicaraguan healthcare is discussed. This history is presented as a reflection of the sporadic nature of the Nicaraguan political environment over the last 30 years. The changes in healthcare policy over this time period are then examined through the lens of the hospital’s healthcare providers. Perspectives on public vs. private systems, the limited ability to affect reform, and motivations behind entering the medical profession are analyzed as they pertain to job satisfaction of healthcare workers.

Hanna Higginson, University of Utah Anthropology The study, “Sentimentality Preserves Our Past” focuses on two branches of nostalgia: sentimentality and historical utility and their relationship to cultural relics. Our approach assumes we can recognize similarities and differences between cultural communities by describing the extent to which individuals are sentimental or utilitarian with regard to their possessions. We are currently collecting data from the northern Utah community and Utah Tongan community. We set up a preliminary exercise asking participants to free list the items they have a difficult time throwing away for sentimental reasons. So far we have collected 40 questionnaires and 13 interviews that focus on the five most referenced items. Our target is to conduct about 45 questionnaires and 45 interviews for each community. For the Utahan population we have enough data to identify an S:U RANK – the ratio between a participant’s measure of sentimentality to the measure of utility. For the 25 questions where a respondent’s answer could be classified as appealing to sentimentality (s) or historic utility (h), we summed the totals then divided it by the sum of the answers appealing to utility (u). All totals above 1 indicate that the respondent prefers protecting items for nostalgic purposes; all answers below 1 indicate the respondent prefers items of utility. The S:U RANK gives a general view into a particular population. From this we can discuss the effect of variables such as age, marital status, children, age of children, and years in Utah on an individual’s preference. Currently, the pattern from the S:U RANK suggests that marital status and children are having the greatest effect on the ratio, but this will become clearer as the interviews progress. The research began as an effort to understand the presence of nostalgia as applied to five common relics among a particular diaspora, in light of the incoming data, this question is developing into an attempt to understand nostalgia in a cultural and universal sense as well.

Melanie Cooke, University of Utah Geography Range Creek is a small deeply incised canyon located in south central Utah. It is a remote canyon that has experienced minimal impact to its rich archaeological heritage by European-American settlers during the last two hundred years. The canyon contains hundreds of important archaeological sites and thousands of artifacts from the Fremont culture that inhabited the area from around 800 A.D. to 1350 A.D. Though work has been done on the archeological sites in the canyon, a better understanding of the paleoclimate and environment are needed to help understand why the Fremont culture disappeared so abruptly around 1160 A.D.Dendrochronology has been shown to provide excellent data for reconstruction of climatic conditions. Conifer tree species, including Douglas fir (Pseudotsuga mensiezii), are well-suited for studying past climate through the analysis of their tree rings. In this study, I use increment cores from Douglas fir to build a local history of tree-ring growth rates through time and will compare these results to regionally available climate records. By comparing changes in ring widths with weather station data, including monthly and seasonal temperature and precipitation records, I hypothesize that Douglas fir will provide a sensitive indicator of past changes in winter precipitation. Twelve tree cores collected from climatically sensitive locations during the 2012 summer are being analyzed from Range Creek Canyon. Preliminary analyses of the tree ring series suggests this study will provide a climatic history spanning the past ~300 years. Although this analysis cannot provide direct observation on climate conditions during the period of the Freemont occupation and abandonment of Range Creek, it will provide insights into the natural climate variability occurring within the Range Creek Canyon. All tree cores are being analyzed with ARSTAN and COFECHA software, commonly used by dendrochronologists, and results will be made available at the time of presentation.

Hanna Winzenried, Brigham Young University History Red hair is one of the most rare hair colors. In fact, only two percent of the world’s population has naturally red hair. My research attempts to show how Western perception of red hair has undergone transformations over time. I seek to show how red hair has been associated with a variety of perceptions from the Middle Ages onward. This is not a comprehensive study, but one that takes a variety of primary sources to show how changing societal perceptions of red hair imbued females (and males) with a variety of traits contributing to a kind of physiognomy of hair whereby a person’s character could be read through hair color.

Belinda Ramirez, Brigham Young University Anthropology The Hmong are a stateless hill tribe ethnic group originating in southern China. Due to persecution and discrimination from the Chinese, many Hmong migrated to the surrounding regions of the Southeast Asian massif in the eighteenth century. The mountainous homes of the Hmong now lay within the borders of countries such as Thailand, Laos, and Vietnam. Religiously, Hmong are traditionally a shamanistic people, believing in spirits and worshipping their ancestors through diverse practices, such as animal sacrifice and spirit calling. In addition to traditional Hmong belief (dab qhuas), many messianic religious groups have recently surfaced within the Hmong diaspora, often accompanied by a prophetic leader, criticisms of traditional Hmong practices, and a hopeful vision of the future in which there exists a Hmong country. My research on this subject is based on an ethnographic field study in Nan Province, Thailand among the Is Npis Mis Nus, a Hmong messianic religious group. Using traditional anthropological field methods, I acquired data on the practices and beliefs of the Is Npis Mis Nus, as well as investigated their conceptions of nationalism, ethnicity, and identity. In this paper, I posit that the rituals and beliefs of the Is Npis Mis Nus reveal the group’s desire for Hmong political, cultural, and economic legitimacy and national sovereignty. These beliefs and rituals also serve as boundaries that provide a clear distinction between messianic and non-messianic Hmong. Additionally, I explain how the characteristics of the Is Npis Mis Nus religion help the Hmong deal with the social and ethnic disruptions that globalization has presented.

Jennifer Mitchell, Brigham Young University History The science fiction television show, Star Trek, deals heavily with political issues ranging from the complexities of war to changing gender roles in American culture. My paper will discuss how the show accomplishes this successfully, particularly through the representation of historical events. The Holocaust is one example of a reoccurring theme throughout the show, an issue that is addressed appropriately when many representations of the Holocaust are so heavily criticized. This paper will examine the themes and structures of different episodes through out Star Trek: The Original Series. One episode to clearly address the anti-Semitic attitudes of the Nazi Regime, “Patterns of Force,” aired on February 16, 1968, a relatively short time after the Regime had fallen. Many of the adult viewers at this time had experienced the Holocaust in their lifetimes, directly or indirectly. An episode entitled “Space Seed” deals with eugenics and the idea of creating a super race. The episode attempts to demonstrate how those in the 23rd century may react to such practices. In “Consciousness of the King” Captain Kirk reveals that he had personally witnessed and been victim to genocide. Not only does genocide clearly connect to the Holocaust, William Shatner (the actor who portrays the Captain) is in fact Jewish. His costar Leonard Nimoy (Spock) also comes from Jewish heritage. Having two Jewish actors in leading roles adds yet another level of complexity when portraying such sensitive material. The paper will take this into account as well as other complications of representing the Holocaust in popular culture.

Nathan Gill, Brigham Young University Geography The Africanized honeybee first entered southern Utah within the last five years. This invasive species reduces pollination and honey production and is therefore costly to the farming industry and local apiaries. Additionally, Africanized honeybees are extremely aggressive and pose a threat to the health and well-being of Utah’s residents. The aim of my study is determine if Africanized honeybee habitat in Utah can be accurately modeled through remote sensing and Geographic Information Systems analysis. Locations of known Africanized honeybee presence and absence, acquired from the Utah Department of Agriculture and Food’s ground reference data, were used to identify the characteristics of suitable habitat. Criteria that we considered include minimum temperature, precipitation, distance to water, elevation, slope, aspect, and Normalized Difference Vegetation Index, among others. I have identified the conditions for suitable habitat and I am in the process of designing a model to identify Africanized honeybee habitat across Utah. After the model has been refined, it will be tested against additional locations of presence and absence acquired from the Utah Department of Agriculture and Food. The modeled habitat will help the Utah Department of Agriculture and Food and other organizations to focus efforts to educate the public about the dangers of Africanized honeybees in specific locations that are at high risk.

Charles Ankenman, Brigham Young University Spanish and Portuguese The Middle Ages and the cultural, political and social coming of age of the European continent produced a corpus of literature and a literary tradition representative of its religious and cultural values that, amazingly, still continues to influence society today. Commonly employed terms such as “a knight in shining armor”, “prince charming”, and parallels occasionally drawn between children and both literary as well historical princes and princesses of yore are evidence of the longevity of the influence of Medieval culture, it’s ideals and the literary genre that most perfectly reflected it: the novel of chivalry. Today, few academics study the genre, it is seldom analyzed in universities, and almost never read outside of academia. In fine, their legacy and their memory has largely been preserved in the endearing pages of Cervantes’ masterpiece, Don Quixote. In this work, the protagonist, don Quixote, goes mad as he credulously devours his extensive private collection of chivalric literature believing all he reads to be not only true but also worthy of imitation. Nevertheless, despite Don Quixote’s wide reading public, few have ever read a novel of chivalry and, as a result, the exact definition of the novel of chivalry can be both obscure and even beyond the grasp of the modern reader who is uninitiated in the field of Medieval and Renaissance chivalric literature. Nevertheless, through a careful analysis of don Quixote’s private collection of novels of chivalry, the mega-genre that they constitute and the sub-genres that comprise it; the chivalric genre can be clearly defined. Additionally, through the comparative analysis of the English term novel of chivalry as opposed to the Spanish term libros de caballerias (book of great deeds done on horseback) the importance of language’s influence on the perception of the world will be demonstrated and Jacques Lacan’s theory regarding language and perception substantiated. Finally, this analysis will elucidate the novel of chivalry’s status both as a continuation of the great classical epic tradition as well as another link in the universal tradition of epic poetry and storytelling. This presentation will be of interest both to Medievalist as well as specialists in the Renaissance, Don Quixote, Spain and Portugal. Power Point Presentation. 20 – 30 minutes.

Duke Cruz, Westminster College Philosophy This research focuses on the nineteenth-century philosopher Friedrich Nietzsche, his philosophy, and how that relates specifically to what he thinks about music. In general this presentation will consist of three main parts: 1) analyzing passages on music in a few of Nietzsche’s works, specifically, “The Birth of Tragedy”, “Beyond Good and Evil”, and “Twilight of the Idols’” with supplementary material relating to his philosophy taken from “The Anti-Christ”, and Walter Kaufmann’s biography of Nietzsche “Nietzsche: Philosopher, Psychologist, Antichrist”; 2) showing the relation of music and reason in each example; 3) postulating the importance of this relation. Throughout my research on Nietzsche and his thoughts surrounding music, it is apparent that whenever he discusses music, it is never about the specific score, keys, melodic content, etc. Instead, I found that in most of the passages that I have ran across, Nietzsche’s thoughts on music collide directly and importantly to his philosophy. In this presentation I aim to elucidate what the importance of this relation between music and reason could be for Nietzsche.

Anna Maria Guadarrama, Utah State University Languages, Philosophy, and Communication Studies Guerrilla warfare is an ancient style of armed conflict that continues to be practiced throughout the world today. Many countries and peoples have either taken part in guerrilla warfare or have sought to counteract irregular war with counterinsurgency methods (e.g. United States). This thesis project consists of an analysis of the guerrilla warfare theories and battlefield strategies utilized by Ernesto ‘Che’ Guevara—the Argentine-born Cuban revolutionary considered by most people as the premier thinker on the nature of guerrilla warfare. Guevara developed his foco theory of guerrilla warfare based on the model of warfare used successfully during the Cuban Revolution, and then sought to replicate this achievement in The Congo and Bolivia. After a brief introduction on the nature of guerrilla warfare in general, the thesis proceeds to analyze the theoretical writings on guerrilla warfare by Ernesto ‘Che’ Guevara, as seen in his seminal treatise Guerrilla Warfare: A Method (1963). The thesis then proceeds to analyze Guevara’s application of his foco theory as an on-the-ground guerrilla leader to evaluate the praxis of Guevara’s foco theory during his guerrilla campaigns in Cuba, The Congo, and Bolivia.

A. Emma McFarland, University fo Utah Languages and Literature The Romance of the Rose of Guillaume de Lorris recounts the story of a dreamer who, wandering through a garden, encounters a rose and is overcome with desire for it. While it is undisputed that The Romance of the Rose is an allegory of love, the kind of love it portrays and the stance it takes on the matter remain hotly debated. Is The Romance an ideal depiction of “courtly love” where the rose is the woman admired? Is it an erotic tale of the conquest of desire, the rose symbolizing forbidden sexual aims? This paper conceptualizes The Romance of the Rose as the lover’s quest to attain his erotic desire within the confines of a system of courtly love that valorizes fin amor over fol amor. Romantic love and erotic desire are irreparably alienated from one another within medieval courtship and, here, Guillaume seems to use his allegory to elucidate the harms inflicted by this courtship system. Juxtaposing the woman Rose with the textual image of the rosebush, we see the violence incurred by this love object that has been both exalted into oblivion and objectified into bits. This paper traces the dissection of the rose image, reads the rose as a euphemism of sexual aims, and studies the God of Love as the personification of courtly love’s enforcement. In the realm of The Romance of the Rose, to love is to suffer and both lover and loved are relentlessly subjugated to violence.

Katie Patterson Hulett, Dixie State University English, Literary Studies emphasis In the last pages of her memoir, The Woman Warrior: Memoirs of a Girlhood Among Ghosts, Maxine Hong Kingston tells the story of the Chinese poetess Ts’ ai Yen, a woman captured by

Summer Mosgofian-Barry, Dixie State University English-Secondary Education Even avid readers of J. R. R. Tolkien’s work may not recognize how extensively his scholarly pursuits and deep knowledge of ancient languages and legends inform his fantasy writing. As a scholar who not only gave new insight into the art of Beowulf, but also as one who proved the existence of a remnant of Old and Middle English untouched by the Norman conquest, he used his mastery of Germanic languages “Old and Middle English, Old Norse, Old Finnish, Welsh” and even his familiarity of Proto-Indo-European vocabulary, to create linguistic and narrative elements in fictional works like The Hobbit and The Lord of the Rings trilogy. The connections between his scholarly pursuits and the characters and languages he invented, such as those between Anglo-Saxon syntax and mythology and Tolkien’s idealized Anglo-Saxons, the Rohirrim, as well as those connections between Snorri’s Edda and the Elvish language Quenya, clearly demonstrate his acumen as a philologist. In fact, some of Tolkien’s Middle Earth legends were clearly inspired by his extensive knowledge of, and are even modeled after, ancient writings and legends, including Beowulf, The Wanderer, “The Maid of the Moor” and Grendel, while he also utilized kennings like those seen in, again, Beowulf, “Caedmon’s Hymn”, and Snorri’s Edda. This paper looks at multiple, though certainly not all, of Tolkien’s use of early language forms and legends and in doing so, delivers the following conclusion: Tolkien’s extensive scholarly work and love of many languages, as well as his passion for the mythology of those languages, clearly plays an integral part in his fiction.

Melissa Lewis, Dixie State University English Both George Eliot and Thomas Hardy have been called determinists partly as a result of their novels The Return of the Native and The Mill on the Floss. The role of fate or destiny largely guides the plot of these works, regardless of the decisions and merit of the characters. A comparison of the style of determinism between the two authors reveals that George Eliot emphasizes a moral force while Thomas Hardy focuses on a force associated with nature. Eliot’s determinism suggests that the world is determined but that humans still remained morally responsible for their actions, as Maggie is responsible for not feeding Tom’s rabbits or for her lack of decision as she drifts down the river with Stephen Guest in The Mill on the Floss. Hardy tends to be more of a naturalist. The naturalist is a product of post-Darwinian theory that humanity is controlled entirely by nature. For example, the fate of Eustacia Vye in The Return of the Native seems to be controlled by the hand of Egdon Heath. Both novels depict extraordinary characters in a world bent to squash them. These novels reveal that the individual exists as a solitary entity, and the relationship that used to exist between God and individual is now between the individual and society. Both Eliot and Hardy create novels where the world seems determined and cruel and characters that don’t adhere or fit in this mold are eliminated or reduced.

Kendra Williamson, Brigham Young University Linguistics and English Language Questions regarding language policy have persisted in Intensive English Programs nationwide. BYU’s English Language Center has revoked an English-only policy in favor of an English-mainly policy. In this environment, a two-month study has been initiated in which four experimental-group classes are provided tools for self-regulated learning to encourage English use during the lunch break. Students record goals and perceived actuals daily, reflect and respond to their individual progress, and receive weekly printed progress charts. As students use tools designed for self-regulated learning, it is expected that their daily efforts to speak English and their speaking proficiency test scores will improve.

Susie McGann, Brigham Young University Humanities As the American economy changes, jobs are becoming increasingly more focused on teamwork, collaboration, and analytical and social skills in addition to technical skills. My initial hypothesis is that such knowledge and skills can be located in the category of a capacity for leadership–leadership of a sort that is of value even among entry-level professionals. These skills can be acquired through study in the Humanities. With my research, I hope to prompt change in the existing culture among students majoring in these fields so that they will begin conversations about careers earlier and more frequently among fellow students and faculty. In order to reach these goals, I collaborated with other humanities students to compile a group of individual essays that explain to undergraduates in Humanities majors how their study is preparing them to be leaders. I reviewed and edited these papers, and researched and wrote a supplicant formal essay to address current or prospective students in Humanities disciplines. The collection of papers are accessible to Humanities students through the College of Humanities as a resource written by students in their fields that explains the value of a Humanities education for life after college. I also organized and hosted an event for undergraduate Humanities students to begin a college-wide conversation about their Humanities degrees in terms of leadership.

Madison Mercer, Brigham Young University Anthropology Between 1964 and 1969, Spotten Cave near Goshen, Utah was excavated by Brigham Young University (BYU) archaeologists. In 1971, James M. Mock published the only official report dedicated to this site as his Master’s Thesis. This cave contained valuable artifacts that can allude to the diet and medicinal plants of the Fremont Indians and other Utah prehistoric groups. Such plant knowledge is scarce throughout the Eastern Great Basin, even among those cultural groups where much is known. Through a comparison study called ethnohistory between those living now, such as modern Gosiute, Ute, and Shoshone, with what plants have been found in human coprolites (fecal remains) from Spotten Cave, it is possible to recon- struct what plants prehistoric people may have used for food or medicine. This will provide greater insight into who lived before in the Eastern Great Basin and is helpful knowledge for those wishing to study how the Utah environment was utilized in the past.

Laura Hatch, Brigham Young University Humanities, Classics, Comparative Literature Art historians and artists have long treated linear perspective solely as an artistic technique. As defined by the Italian Renaissance, linear perspective is a tool to recreate reality on a canvas. But linear perspective carries philosophical implications, as well. Albrecht Dürer, a German Renaissance artist, seems to have understood perspective’s limitations in truth-making. In reading this image with a knowledge of Heideggerian phenom- enology, I propose that while on the surface this woodcut may seem to be nothing more than an illustration of proper technique, Dürer is actually exposing linear perspective’s failure to capture and truly represent an image as reality.

Megan Barber, Brigham Young University History This project investigates the British view on the Civil War in the United States. Specifically, it explores the correspondence between Richard Lyons, the British envoy to the United States, and Lord Russell, the British Secretary of State for Foreign Affairs. It charts the changing perception of Lyons’ view of the Civil War as it progressed during the first crucial year of the conflict. The Confederacy actively sought British recognition and in fact sent two representatives to the United Kingdom who were subsequently seized by a Union ship. Lyons’ role in what came to be known as the Trent Affair is known, but has overshadowed his other perceptions of the conflict. This project draws on the original PRO manuscript correspondence held in the UK National Archives in London.

Dakota Hawkins, Westminster College Arts and Sciences Abstract. Previous studies have documented effects of urbanization on the behavior, reproduction and survival of wildlife. Specifically, noise pollution in urban areas has been known to mask communication among several avian species. In a previous study in Mexico City, House Finches increased the frequency (pitch) of their songs to help mitigate the effects of low frequency urban noise. To document the average minimum frequency of House Finch song in Utah, we recorded House Finches singing from May 2012 to August 2012. Three sample sites with 1 km radii were established in Salt Lake City, Utah while a fourth site was sampled in Logan, Utah. Ambient sound was recorded at locations where songs were recorded to measure urban noise. Average minimum song frequencies and ambient noise were calculated for three sites. Frequency measurements were not significantly different among the three urban populations. Future studies will compare the minimum frequency of these urban populations to nonurban populations and investigate syllable structure and use.

Graham Doherty, Westminster College Biology The Great Salt Lake is home to Artemia Franciscana, a species otherwise known as brine shrimp. These halophiles are able to live in extreme environments that have higher than average salt concentrations. Brine shrimp live their entire life cycles in the Great Salt Lake and can be found in both the north and south arm of the lake. Currently, the genetic information regarding the brine shrimp populations at different locations in the lake is underdeveloped. The Artemia populations live in different microenvironments throughout the lake with different physical characteristics and barriers between one another. The physical environment also affects the salt concentration in each microenvironment. Other studies have shown that the increased salinity of an environment accelerates the rate of change in the mitochondrial genome. We feel that different salinity levels in each microenvironment will create different amounts of genetic variation. Our goal is to determine if the distribution of genetic variation is different at different sites in the Great Salt Lake Artemia population.

Natalie Batty, Westminster College Biology Hydrocarbon metabolizing microbes play a critical role in bioremediation and bio-augmentation projects. In 1978, Brock et al. identified microbes in the Great Salt Lake (GSL) capable of metabolizing hydrocarbons, but since then these microbes have gone unstudied. This research has isolated and identified bacterial species capable of hydrocarbon metabolism from GSL. Though capable of survival in the high salt concentration of the Great Salt North Arm, we have demonstrated that these species are also capable of thriving in low salt concentrations. This research will continue with the characterization process to identify what hydrocarbon sources each species is capable of degrading.

Alisha Ryan, Weber State University Microbiology One of the major challenges facing U.S. military caregivers is the presence of multidrug resistant organisms in extremity wounds. The most frequently identified drug resistant strains of bacteria found in these wounds are Acinetobacter baumanni, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus. Due to these organisms rapid increase in resistance to the commonly used drugs, it is crucial to discover and establish alternative methods for treating these microbial infections. Antibiotics are currently the most common treatment for infections by these pathogens, and there is little data on the evaluation of phytochemicals as potential chemotherapeutic agents that could take their place. We have screened 24 individual compounds from 9 major compound families to determine if plant-based phytochemicals could be explored further for use in treating bacterial infections in patients with military wounds. All compounds were tested to determine the minimum inhibitory concentration (MIC) and minimum lethal concentration (MLC). Of the 24 compounds tested, 21 (87.5%) inhibited at least one strain used in this study, with only 1 of the 24 (4.2%) inhibiting all strains. There were 10 (41.7%) of the compounds that displayed MIC values less than 100 _g/ml. For compounds displaying MLCs, they ranged from 2.5 mg/ml to 78.13 g/ml. While there is much more research that needs to be done with each of these compounds, this work is a crucial first step in the drug discovery process. We believe that several of these may serve as potential novel inhibitors of these drug-resistant bacteria.

Lauren Johnson, Weber State University Microbiology Bacteriophages in aquatic environments play a significant role in bacteria population control, as well as recycling nutrients. The bacterial genus Halomonas is commonly found in the Great Salt Lake (GSL), but very little is known concerning its population dynamics. This euryhalophilic genus is highly versatile concerning its ability to grow in a wide range of substrates and environmental conditions including salt concentration. To better understand GSL microbial ecology, seven strains of Halomonas were isolated from the GSL and identified using 16S rRNA. Samples of South Arm GSL water were filtered twice through a 0.2 m filter, and tested against these Halomonas strains using soft agar overlays to detect Halomonas phage. Three strains exhibited plaque formation indicating the presence of phage. Halomonas phage isolates produced very small plaques, sometimes barely visible. Individual phages were isolated by vortexing agar plugs taken from single plaques in sterile saline then filtering through a 0.2 m filter. From host range streak plates, a single phage isolate (LJ17) appears to infect 4 closely related Halomonas strains. Electron micrographs of LJ17 phage indicate it has a small icosahedral head and perhaps a very short tail. There also appears to be a satellite phage that may be associated with LJ17. There are no reports of Halomonas phage isolated from the Great Salt Lake (GSL), although there are phages found for marine Halomonas strains. Successful isolation and characterization of novel GSL Halomonas phage, besides being critical for development of host/phage models, will also allow studies of GSL microbial ecology.

Robert Putnam, Utah Valley University Biology Pannota, the fused back mayflies, is divided into two main lineages: Ephemerelloidea and Caenoidea. Most of the pannote families are relatively small, in terms of number of genera, with the exception of the larger family Ephemerellidae with 32 genera. Our objective was to 1) test the monophyly of the superfamilies; 2) investigate the relationships within the families of the Pannota as a whole. We combined DNA sequence data with newly generated data (total of ~6 genes) for around 50 taxa. We used standard PCR and DNA sequencing protocols to generate the data. The molecular data were aligned in Muscle and subsequently phylogenetic trees were reconstructed under Parsimony, Maximum Likelihood, and Bayesian frameworks. The results strongly supported the monophyly of the superfamilies, while the relationships of the families within Ephemerelloidea were not resolved.

Emily Matis, Utah Valley University Exercise Science Introduction:

Chin Yiap Tan, Utah State University Nutrition and Food Sciences The long term objective of this research is to explore oil-based materials that can be used to replace trans-fats in the food industry. Vegetable oils such as olive (OO), corn (CO), soybean (SBO), sunflower (SFO), safflower (SAFO) and canola (CAO) were mixed with sunflower wax (SFW), paraffin wax (PW), and beeswax (BW) at different concentration levels (0.1%-100%). Crystal Morphology, oil stability, thermal behavior and viscoelastic properties were studied in this research. Results in our laboratory showed that the crystallization occurred sooner when the concentration of waxes increased. For example, crystallization in 0.1% of SFW in SFO occurred after 24 hours of incubation at 25°C, but when concentration increased to 0.25% the crystallization was observed at the first 15 minutes of measurement. In addition, a change in the thermal behavior of wax/oil soft materials was observed when the concentration of waxes increased. For example, the enthalpy change (H) in SFW/ SFO materials increased from 0.87 J/g at 1% of SFW to 21J/g at 10% of SFW. This increase in enthalpy indicated that the amount of crystal increased when the concentration increased. Overall, the crystallization behavior in wax/oil materials was affected by wax concentration and chemical compositions of oils and waxes.

Monica Cervantes, Utah Valley University The central research question of this project was to determine if high-frequency ultrasound is sensitive to tissue pathology at the microscopic level. Previous studies on surgical specimens have shown that high-frequency ultrasound may be sensitive to a range of breast pathologies including fibroadenomas, atypical ductal hyperplasia, fibrocystic changes, and carcinomas. The ultrasonic parameters that were sensitive to pathology were the number of peaks (the peak density) of the first-order spectra of the waveforms (one forward Fourier transform), and the slope of the second-order spectra of the waveforms (two consecutive forward Fourier transforms). The ability to determine pathology rapidly and with minimal specimen preparation would make high-frequency ultrasound particularly well-suited for real-time use during cancer surgery to ensure all of the malignant tissue has been removed. The purpose of this research was to determine the sensitivity of the peak density and spectral slope to tissue microstructures other than those found in breast cancer. The results of this study would not only support the results from the breast cancer studies, but also extend those results to the detection of cancer and other diseases in a range of organs and tissues. The research methodology included the following steps. (1) Freshly excised bovine organs were obtained from a meat packaging facility, including the heart, liver, and kidney. (2) Specimens approximately 3x3x1 cm in size were dissected from the organs and tested immediately with ultrasound. (3) Both pitch-catch and pulse-echo waveforms were acquired from the samples. (4) The data were analyzed by determining the peak densities and spectral slopes. The results showed that the more heterogeneous tissues of the heart, the vascular structures (aorta, vena cava, etc.), displayed significantly higher peak densities than the muscle tissues. Similarly, the ureter, which has greater heterogeneities in its structure (larger and more varied), displayed significantly higher peak densities than the cortex and medulla tissues. No significant trends were observed for the liver tissue, or for the spectral slopes except for kidney medulla tissue. Heterogeneity and peak density in high-frequency ultrasonic spectra that may be useful for performing real-time pathology during cancer surgery.

Sam Hawkins, Utah State University Biological Engineering Colorectal cancer (CRC) is one of the most frequently diagnosed cancers in women and men. It is often treatable if caught early. However, tumors may metastasize which can result in a poor prognosis. A better understanding of the tumorigenesis and evolution of metastatic tumors in CRC patients could lead to earlier diagnosis, pre-emptive screening, and a better outcome. Copy number analysis of primary tumor tissue has revealed genes associated with colon cancers, but a comparison between primary and metastatic tumors has never been done. Normal tissue, primary tumor tissue, and metastatic tumor tissue was collected from twentyfive individuals. Copy number alterations were determined by microarray data generated from Molecular Inversion Probe (MIP) technology (Affymetrix, Inc.) for copy number analysis using Nexus software (BioDiscovery, Inc.). Metastatic tumor samples show a greater rate of copy number alterations (CNAs) from the primary tumors and even more alterations from normal tissue samples. Certain regions of the metastatic genome show high rates of CNAs whereas the primary tumor genome does not. These areas are key regions for potential understanding into the molecular origins of metastatic tumors. Understanding specific regions and genes with CNAs in metastatic tumor samples may lead to further research in cancer genetics and possible target areas for pharmaceutical testing.

Kat Combs, Utah State University Plants, Soils, and Climate Kentucky bluegrass (Poa pratensis L.) is a commonly used turfgrass species with many varieties being sold around the world. However, those varieties are very difficult to tell apart morphologically. Our objective was to use genetic markers (primers) to identify varieties, even if they are visually similar. This is valuable to the turfgrass industry for plant variety protection. We also wanted to use this data to explore the apomictic tendency (clonal reproduction) of the varieties. We collected leaf tissue from 24 Kentucky bluegrass varieties, extracted DNA, and sequenced portions using 29 EST and 21 genomic primers. This data was used to determine genetic relationships using a neighbor-joining dendrogram. Similarities of the genetic sequences from the varieties were estimated using the DICE coefficient. We found more polymorphisms in genomic primers than in EST primers with high variability between the varieties. Both types of primers were robust enough to distinguish varieties and that each variety was unique and genetically distinguishable. In addition, we discovered some varieties had large amounts of variation within a variety. This was unexpected due to the usual apomictic nature of the species. The markers resulting from our research will be available to the turfgrass industry.

Tyler Shimko, University of Utah Biology Differences in the genomes of organisms control an organism’s ability to deal with and adapt to environmental stresses. In this project, two strain isolates of the nematode Caenorhabditis elegans were analyzed using high-throughput assays measuring growth and offspring production to determine the genes that confer a greater resistance to the herbicide paraquat. Paraquat acts by interfering with electron transport mechanisms within the cells of living organisms. This mechanism not only allows it to act as an effective herbicide, but also causes it to pose a considerable risk to the health of animals, including livestock and humans. Using statistical genetics, regions of the genome were identified that are likely responsible for differences in growth rate and fecundity observed in the two strain isolates when grown in paraquat. Near-isogenic lines and extra-chromosomal arrays were then created to isolate these portions of the genome in a control genetic background. This approach allowed us to be able to attribute any differences in the two traits to the genes contained within the intervals. After analyzing the body size data, representing the growth of the animals over 72 hours, we were able to draw a preliminary conclusion that an interval on chromosome V may have a small but significant effect on growth determination. As a result of this project, a specific interval was identified that may be responsible for a greater growth rate, three near-isogenic lines were created, and 34 extra-chromosomal arrays were generated. This work will be used in the future to identify the gene(s) responsible for the greater growth rate and fecundity observed in some animals exposed to the herbicide paraquat. These results will allow us to draw conclusions about the roles that these genes, and others like them, play in an organism’s ability to cope with environmental stresses.

Alexa Provancha, University of Utah Exercise and Sport Science Impaired endothelium-dependent vasodilation has been associated with various cardiovascular diseases, including heart failure, is linked to oxidative stress, and persists despite heart transplantation (HTx). PURPOSE: This study aimed to determine if changes in vascular function following HTx can be assessed using incremental handgrip (HG) exercises to induce nitric oxide-dependent vasodilation of the brachial artery. Furthermore, the efficacy of an acute oral antioxidant cocktail (AOC) to reduce oxidative stress and improve vascular function in this population will be assessed. METHODS: 31 HTx recipients (14 years post-HTx) and 10 healthy age-matched controls were given either and AOC (Vitamin C, E, and alpha-lipoic acid) or placebo (PL; randomized for the subjects’ two visits. Measurements of the brachial arterial blood velocity and vessel diameter were performed during three absolute workloads (4, 8, and 12 kg) of HG exercise using ultrasound Doppler. RESULTS: Maximal vasodilation during PL HG exercise was not different between the controls (8.2 ± 1.5%), and recent (< 3 years post) HTx group (8.5 ± 1.2%), but the 5-10 years post-HTx groups had a tendency to be lower (6.5 ± 1.9%). The > 14 years post-HTx group recipients (5.2 ± 1.9%) were significantly attenuated compared to both the controls and the recent (< 3 years post) HTx recipients. CONCLUSION: The study supports the use of HG exercise as an assessment of vascular function in a patient population with known cardiovascular risk. Additionally, these results suggest that vascular function is similar between controls and early HTx recipients but declines as time passes following surgery despite normalized cardiac function.

Kandrie MyIroie, University of Utah Human Genetics In C. elegans, sperm activation is the process through which sperm become motile. Genes that regulate sperm motility are being identified through a genetic screen. In this screen, swm-1 mutants that have sperm that activate too early are mutagenized, and genes regulating sperm motility can be found when the sperm of mutant worms no longer activated too early. Two genes have already been identified, try-5 and snf-10, and these can help identify new mutations. By selecting specific strains with sperm activation defects, the mutations causing the defect can be characterized. To sort through the many mutants, complementation tests to identify them as a new gene, or a new allele of try-5, snf-10 are being done. Selectively crossing an unknown mutant strain to a strain with a known mutation in try-5 or snf-10 will help identify the unknown mutation. If the progeny of the cross have activated sperm then the two strains complement each other. In this case the alleles must be in different genes and the unknown mutation from that cross has not been identified. If the progeny instead has non-activated sperm then the mutations failed to complement and the unknown mutation is allelic to the mutation it was crossed with. One new allele of try-5 and of snf-10 has been found. It is suspected that the other strains contain alleles of new genes not previously identified. Further complementation tests will be done to test this theory.

Merima Beganovic, Westminster College Molecular Biology Nuclear Pore Complexes (NPC) create aqueous channels embedded in the nuclear envelope and are made from a network of proteins called nucleoporins (nups). The nucleoporin Nup153 has previously been found to be required for proper assembly of the nuclear lamina. In this study, a fragment of Nup153 was overexpressed in order to impede Nup153 function in T-Rex HeLa cells. After inducing the expression of the dominant negative fragment, I examined the localization of lamins A, B1 and B2, as well as SUN1, Emerin, and BiP, three proteins that mark cell membrane compartments. Lamins B1, B2, SUN1, and Emerin were found to mislocalize to the cytoplasm of the cells, and colocalization among the proteins was observed. Lamin A also had an abnormal phenotype unlike that seen with the B lamins, but indicative of a problem with integration of Lamin A into the nuclear lamina. Colocalization of the various lamin isoforms with membrane proteins such as SUN1 and Emerin indicates a problem with membrane assembly. The distinct localization of BiP, however, suggests that there is a “nuclear-like” membrane in the cytoplasm that either does not incorporate normally into the nuclear envelope as it forms or is newly-recruited to mislocalize the lamin proteins.

Chelsie Thomas, Weber State University Zoology The invertebrate ribbon worm, Paranemertes peregrina, serves as a unique model in that it thrives in an environment exposed to drastic fluctuations in salinity during tidal interchange. Its ability to sustain homeostatic integrity is not well understood. With an aim to clarify this phenomenon, total RNA was isolated, and reverse transcription with polymerase chain reaction allowed us to serendipitously clone and elucidate a 488 base pair region of a gene coding for a Tachykinin Receptor (TKR), a subtype of G-protein coupled receptor (GPCR). This region shows 79% homology to the mouse TKR-2 mRNA sequence, and 44% homology to human Neuromedin-K receptor’s amino acid sequence. Specifically, we have isolated a portion containing a cytosolic carboxy-terminus that has classically been associated with palmitoylation or otherwise hydrophibicity-enhancing interactions. This process facilitates the docking of cytosolic subunits to the membrane in the assembly of GPCRs, acting as a regulatory component. In vertebrate models, much of these proteins are conserved. Given that only a few of these genes have been reported for invertebrates, this suggests a critical need for investigation of the evolution of TKRs as they relate to stress response. Additionally, this receptor poses questions about its potential role in pain, with the prospect of revealing insight about the long-elusive pain perception in invertebrates.

Brooke Nelson, Weber State University Interior Design According to the National Substance Abuse Index (2006), Utah women account for 31.7 % of the entire drug abusing population. Mind-altering substance abuse among women creates many unique problems in their lives according to Wesa and Culliton (2004). Additional research by Wesa and Cullliton shows this can affect their futures, finances, families, health, and relationships. Many of the women have other underlying problems that lead them to use mind altering substances to cope with these problems as researched by James (2011). In the Ogden, Utah area, mind-altering substances have created an epidemic that needs to be helped. Serenity, A Drug Abuse Recovery Center, will be an 18,000 square foot building located in Ogden, Utah. Serenity helps facilitate mental and physical healing to create an overall wellbeing for the women. Research has said that women are more likely to receive help for mental conditions than the substance according to Greenfield (2006). Mental disorders and substance abuse will be addressed at the recovery center. Individual and group therapy methods will be used because of their effectiveness shown by Greenfield. Research by Greenfield has shown that women in treatment centers have a greater chance of retention if dependent children are present. Serenity will provide a private room for a woman and two dependent children to stay at the center. The center will include nutrition education and dining area, and a children’s area. The children’s area will have reading, playing, learning, and therapy treating areas.

Jennifer Jorgenson, Weber State University Microbiology Enterococcus, a bacterial genus that normally inhabits the gastrointestinal tract of animals, can be pathogenic to humans, causing urinary tract infections, sepsis and other serious diseases. It is also one of the major causes of hospital acquired infections. One important complication of those infected with Enterococcus is the fact that they often have a high level of antibiotic resistance, making effective treatment of patients more difficult. While it is a normal inhabitant of the gastrointestinal tract, it can survive outside its host in the environment, even in adverse conditions, such as the Great Salt Lake. In this experiment, isolates of Enterococcus were collected from the Great Salt Lake and from fresh water sources. These isolates were tested for different phenotypic characteristics and for their antibiotic resistant patterns against five antibiotics. The results of the Kirby Bauer disk-diffusion assay demonstrated that 47% of enterococcal isolates from the Great Salt Lake were resistant to one or more of the five antibiotics. In contrast only 15% of Enterococcus isolated from the fresh water source were resistant to one or more of the five antibiotics. This has implications for those who have recreational and occupational contact with the Great Salt Lake.

Gessica Stovall, Utah Valley University Exercise Science Introduction:

Miguel Cuevas and Joseph Wilkerson, Utah Valley University Biology Ovarian cancer is the leading cause of death from gynecologic malignancies in the United States and is the fifth leading cause of cancer death among American women. It is estimated that over 22,000 women in 2012 will be diagnosed with ovarian cancer in the United States and approximately 15,500 women will succumb to the disease. This is due to the fact that only 20 percent of cases are diagnosed before the cancer has spread to the peritoneal cavity. Currently, there are no reliable, standard screening tests; the only diagnostic test currently available is the CA125 tumor antigen blood test. This test is inadequate and not available as a general screening tool; additional diagnostics are required to effectively diagnose this disease. It has been previously shown that the tight junction protein Claudin-16, found only in normal kidney, is aberrantly expressed in epithelial ovarian tumors. Therefore, this protein is a good candidate for ovarian cancer diagnostics and targeted therapy. By identifying the promoter region that controls cldn-16 gene expression in ovarian tumors, we can create a luciferase reporter assay to identify cells that express Claudin-16 in culture. To do this, PCR-amplify of various upstream regulatory elements previously identified in kidney cell lines were subcloned into the pGL3 luciferase reporter vector. A higher amount of luminescence is present if the promoter sequence successfully up-regulates the luciferase gene in the vector. This is measured using a Dual Luciferase Assay to determine which promoter region is responsible for the over-expression of Claudin 16. Promoter activity was verified in kidney cell lines that normally express Claudin-16. Next, the assays will be repeated in ovarian cancer cell lines known to express Claudin-16 compared to cell lines that do not express the protein. The promoter assay will then be tested on a collection of ovarian cancer cell lines to determine if luciferase activity correlates with Claudin-16 expression. Once validated, we can test our construct as a cell based assay for identifying therapeutics that can lower Claudin-16 expression in ovarian cancer cells.

Jordan Goodman, Utah State University Biological Engineering Nanotechnology is revolutionizing imaging techniques, antibiotic therapy and cancer treatments. Nanoparticles (NPs) are also utilized in many commercial products such as sunscreens, paints, ceramics and semiconductors. Consequently, it is inevitable that NPs find their way into the environment. The effects that NPs have on agriculture and soil ecosystems are the focus of this project. Metal-oxide NPs such as ZnO are toxic to many bacterial pathogens but the beneficial root-colonizing isolate, Pseudomonas chlororaphis O6 (PcO6), has high tolerance. This bacterium represents an important group of micro-organisms that colonize plant roots improving their resilience to both abiotic and biotic stress. At sublethal dose, the ZnO NPs remodel the secondary metabolism of PcO6 in ways that could have an impact on agricultural ecosystems. Formation of antibiotic phenazines produced by PcO6 is strongly inhibited by ZnO NPs. Phenazines are important for PcO6 growth in biofilms, induction of tolerance mechanisms in the colonized plant, and antagonism of other rhizosphere pathogens. A role of NPs as a point source for soluble metal release is involved in these processes but does not explain the total effect of the NPs. The sublethal effects of the ZnO NPs on bacteria are similar to changes reported by sublethal doses of traditional antibiotics on human pathogens. These changes have a strong impact on fitness of bacterial pathogens, the most significant of which is induction of antibiotic resistance. Although many metal-containing NPs are being used in antimicrobial formulations, these alternative antibiotics too may be inducers of antibiotic resistance.

Katie Fisher, Utah State University Watershed Science Arctic lakes are very sensitive to the effects of climate change. It is important to understand the current food web dynamic and energy flow within these lakes to better understand how they will change in the future due to the effects of a rapidly changing climate. In order to understand the current conditions in arctic lakes, this project consists of an analysis of stable isotopes of carbon (13C) and nitrogen (15N) from invertebrates among fish and fishless lakes in arctic Alaska to compare their trophic level positions and primary energetic sources. In order to carry out this analysis, I collected pelagic invertebrates were collected from 6 different lakes, 3 of which have resident fish populations and 3 of which are fishless. Samples collected in 2011 were analyzed for stable isotope composition by a mass spectrometer at University of California Davis. Results from samples collected in 2012 are pending at Washington State University. I will analyze and correlate the stable isotope results with isotopic data collected from other related projects. With this analysis, I will create food webs to 1) assign trophic positions to each species in each lake and compare those positions across lakes, 2) assess the potential effect fish predation has on pelagic invertebrate community structure. I hypothesize that fish predation will determine zooplankton community structure (e.g., dominant taxa) and alter trophic linkages (e.g., secondary trophic level predation rates).

Anindita Ravindran, University of Utah Exercise and Sport Science Obesity predisposes individuals with Type II Diabetes to cardiovascular complications such as impaired blood vessel function. Due to the elevation of free fatty acids (FFAs) in obese individuals, ceramide, a lipid metabolite, accumulates and might contribute to the inability of a blood vessel to constrict or relax appropriately. Vessel dysfunction is partly caused by the inability of the endothelium, the innermost protective lining of blood vessels, to synthesize and release nitric oxide (NO). Our data indicate that ceramide impairs endothelial NO synthase (eNOS), the enzyme that synthesizes NO. In order to study mechanisms by which ceramide might impair eNOS, it is important to measure cellular ceramide production in response to pharmacological and genetic manipulations. Previously we used P-32 radioactive assays to measure ceramide accumulation. However, the use of radioactivity is expensive, potentially hazardous, and waste disposal is an environmental concern. Therefore, I sought to import a less harmful, more cost effective, yet accurate technique of measuring ceramide production by immunofluorescence (IF). IF allows ceramide to be tagged with a primary antibody which can be detected by a secondary antibody conjugated with a fluorescent dye. I have observed that 250, 500, and 750 uM palmitate (pal) incubation for 3 h increases (p<0.05) endothelial cell ceramide accrual in a dose-dependent manner. Further, a FFA-independent method to alter ceramide accrual i.e., 3 h incubation of cells with N-oleoylethanolamine, also elevates (p<0.05) ceramide production. Importantly, I have shown that 500 uM palmitate-induced ceramide accrual can be prevented (p<0.05) by two structurally dissimilar inhibitors (10 uM myriocin, 1mM L-cycloserine) of the rate-limiting enzyme responsible for ceramide biosynthesis i.e., serine palmitoyl transferase (SPT). None of these inhibitors impairs cell viability. These data indicate that IF is an accurate and reproducible method whereby ceramide accrual can be quantified in endothelial cell systems.

Lee Leavitt, University of Utah Biology Chronic methamphetamine (METH) abuse leads to structural and functional damage in the brain, which likely contributes to cognitive and behavioral dysfunction. Recent data suggest an association between METH abuse and impaired inhibitory control over behavior; that is an impaired ability to inhibit inappropriate actions or thoughts. However, the extent to which METH-induced neurotoxicity is responsible for such impairment remains to be determined. Previously, we reported that rats with METH-induced partial dopamine (DA) and serotonin (5-HT) loss in striatum and prefrontal cortex (PFC) exhibited impaired response inhibition in the form of perseverative responding. Furthermore, levels of serotonin transporter (SERT) binding in PFC correlated with perseverative responding. Herein, we have examined another dimension of response inhibition impulsive action, which is an inability to withhold a “pre-potent” response in rats with METH-induced neurotoxicity. Rats were trained to perform a stop-signal task (SST). Once rats achieved stable responding (>80% correct response) on both “Go” and “Stop” trials, they were treated with saline (0.9%), a neurotoxic regimen of METH (4 x 10 mg/kg, 2-hr intervals, s.c.) under normal ambient temperature conditions (“neurotoxic” METH group) or the METH regimen under cooling conditions (“normothermic” METH group). One week after the treatment, rats were again tested on the SST. After behavioral tests were done, animals were sacrificed and brains removed for determination of monoamine loss. The results showed: 1) the “neurotoxic” METH-, but not the “normothermic” METH or saline-treated rats, showed a 40-60% loss of SERT and dopamine transporter (DAT) binding in PFC and striatum; 2) the “neurotoxic” METH group showed normal behavioral performance in “Go” trials of the SST relative to the “normothermic” METH group or saline controls; 3) the “neurotoxic” METH group exhibited impaired withholding of a “pre-potent” response, as reflected by increased numbers of errors on the “Stop” trials of the SST. Overall, these data suggest that impaired inhibitory control over behavior (i.e., increased impulsive action) can arise as a consequence of METH-induced neurotoxicity to central dopamine and serotonin systems. Supported by NIH grant DA 024036

Andrew Jo, University of Utah Ophthalmology and Visual Sciences All cells, including retinal neurons and glia, must sense and adapt to physical changes in their local environment (e.g. changes in osmotic pressure). Osmotic water flux can cause aberrant cell volume changes, which can contribute to tissue damage, edema, and neuronal hyperexcitability and excitotoxicity. We hypothesized that force-sensitive proteins enable retinal cells to monitor their physical form (e.g. volume) and help maintain homeostasis by regulating cell volume. To test this, we first investigated the properties of cell morphology when cells were bathed in solutions with different tonicities. Under these conditions, we measured changes in cell volume due to osmosis in retinal ganglion cells (RGCs) and Müller glia. We found RGCs were unable to actively adjust their volume, whereas Müller glia reduced their swollen volume in the continued presence of hypotonicity. The regulation of cellular volume often involves calcium signals. We tested whether calcium plays a role in the regulation of retinal cell volume. Free calcium within the cells was sequestered using cytosolic BAPTA, which decreased the extent of hypotonic swelling. This demonstrates that calcium elevations increase the extent of cell swelling. Because cell volume was dependent on calcium, which was elevated by membrane stretch, we hypothesized that the osmosensitive cation channel TRPV4 would transduce osmotic pressure and contribute to cell volume regulation. In an experiment using a selective TRPV4 inhibitor, the extent of hypotonic-induced swelling was reduced. Thus, the opening of TRPV4 leads to a calcium influx that exacerbated cellular swelling. In addition, we tested the idea that TRPV1 cation channel is involved in responses to osmotic stimuli. In an experiment using a selective TRPV1 antagonist, preliminary results show that the extent of hypotonic-induced swelling decreased. This signals that TRPV1 may have a role in volume regulation in retinal neurons and glia. Thus, inhibition of these force-sensitive protein channels might alleviate the deleterious effects of volume changes in pathological contexts. Our findings therefore have implications for our understanding of retinal mechanotransduction and osmoregulation as well as provide a mechanistic framework for developing new therapeutic strategies aimed at blinding conditions that involve mechanical stress and cellular morphology.

Viktor Jiracek, University of Utah Biology The nervous system is responsible for cognition, memory, and motor function. Neurons communicate with each other at intercellular connections called synapses. It is at these locations that vesicles fuse with the plasma membrane and release their contents into the space between the neurons (synaptic cleft). SNARE proteins (synaptobrevin, syntaxin and SNAP-25) facilitate synaptic vesicle fusion by winding together in a four-helix bundle forcing the mixture of opposing membranes. After exocytosis, the cell regenerates vesicles by a process called endocytosis. This process involves the budding of membrane back into the cytoplasm re-forming a functional synaptic vesicle. This progression from vesicle fusion to internalization is termed the “synaptic vesicle cycle” and is coordinated by a long list of molecular players. The SNARE proteins are classically considered to be unique to exocytosis. However, preliminary results from Erik Jorgensen’s lab have implicated SNAP-25 in endocytosis. Although we have evidence that SNAP-25 is required for endocytosis, the molecular mechanism is completely unknown. In this proposal we use forward genetics in the model organism C. elegans to identify novel protein interactions required for SNAP-25 mediated endocytosis. We have designed three suppressor screens that are predicted to target the role of SNAP-25 in exocytosis, endocytosis and general function. The first two screens use crippled forms of SNAP-25 (hypomorphs) while the last screen uses a deletion allele of the SNAP-25 gene (null mutation). With these screens we hope to precisely identify the molecular players involved in SNAP-25 mediated endocytosis versus exocytosis.

Makenzie Hawkins, University of Utah Nutrition Cardiovascular disease (CVD) is the leading cause of death in the United States. Gamma-tocopherol (GTC) has been studied in its relation to hypertension and CVD. GTC has protective effects on the endothelium of arteries; the endothelium is in part responsible for regulating arterial relaxation and reducing blood pressure (BP). Diabetic mice (DB) were chosen for this study because of the associated co-morbidities of hypertension and excess body weight. Eight-week-old mice were divided into 3 separate groups: wild-type fed control (CON) diets (n=12), DB fed CON diets (n=12), and DB fed GTC supplemented diets (0.1%, n=12). BP was measured every week for 8 wk. Body weights were measured every wk and morphometric values were measured at the end of the 8-wk period. There was no statistically significant (P<0.05) difference in systolic and diastolic BP between DB-GTC and DB-CON. There was also no statistically significant (P<0.05) difference in heart rate between DB-GTC and DB-CON. After 8 wk, DB-GTC mice had lower (P<0.05) body weight versus the DB-CON group. In conclusion, a GTC supplemented diet did not result in a change in BP. However, GTC may have blunted weight gain and adipose tissue mass in DB-GTC compared to DB-CON. We speculate this may be due to a reduction of food consumption during the experimental period.

Diego Cordero, University of Utah Biology Many zygotes and spores of brown algae are photosensitive and establish a developmental axis in accordance with directional light cues. Ectocarpus siliculosus is being advanced as a genetic and genomic model organism for investigating brown alga development, and this report investigates photopolarization of the growth axis of mitospores. When exposed to unidirectional light, mitospores photopolarized and established a growth axis such that germination was preferentially localized to the shaded hemisphere of the spore body. The roles of the microtubule cytoskeleton and endomembrane cycling in the photopolarization process were investigated using pharmacological agents. Disruption of microtubule dynamics progressively reduced the percentage of mitospores that photopolarized, while inhibition of vesicle secretion blocked photopolarization nearly completely. Chronic treatment with these pharmacological agents severely affected algal morphogenesis. Microtubules in mitospores and algal filaments were imaged by confocal microscopy. Mitospores contained a radial microtubule array emanating from a centrosome associated with the nuclear envelope. At germination, the radial array gradually transitioned into a longitudinal array with microtubules extending into the emerging apex. At mitosis, spindles were aligned with the growth axis of cylindrical cells in the filament, and the division plane bisected the spindle axis. These studies demonstrate that dynamic membrane cycling and microtubule assembly play fundamental roles in photopolarization, and provide a foundation for future genetic and genomic investigations of this important developmental process.

Dillon Monroe, Southern Utah University Biology When an area is developed no part of that habitat is left unaffected. Urbanization creates many problems for animals by creating physical barriers, fragmenting habitat, introducing completely new habitat, and introducing species. Exotic species are often better competitors for resources and often competitively exclude native species, resulting in population declines and ecosystem disruption. Despite the negative effects of urbanization some species are able to adapt and survive in urban ecosystems. One such species is the wandering garter snake (Thamnophis elegans vagrans). This species is described as a generalist in habitat selection and is often seen in urban communities basking on sidewalks and pavement, living in gardens, and feeding on rodents, insects, and other species. One unique characteristic of this genus is that prey preference has been observed as being a genetic trait and possesses variation, and as such can be modified via natural selection. For my research project I looked at whether prey preference of T. elegans vagrans differs between snakes from three different locations with varying levels of human development. From these three locations in Utah, Tropic reservoir, Monroe (Sevier River), and Utah Lake, we obtained a total of 14 snakes and found that in this group of snakes there is no correlation of prey preference in snakes to level of development of the habitat.

Betty Stoneman, Utah Valley University Philosophy The “American Dream” and the “Working Class Promise” are ubiquitous ideologies in American culture. For this paper, I will argue these ideologies are social constructs which perpetuate and reinforce discrimination, the social hierarchy and the domination of the American working class. First, I will define the key concepts of this paper: ideology, stereotyping, domination, discrimination, the ideologies of the American Dream and the Working Class Promise. Secondly, I will argue these ideologies, as defined by communications professor Kristen Lucas, lead to discrimination and domination of the working class. I will argue the Working Class Promise is an example of philosopher Frederick Nietzsche’s slave morality, where a lower status group attaches positive values to themselves which only serves to perpetuate discrimination against them. Further, I will argue domination arises from such discrimination by examining a study by psychologists Shannon K. McCoy and Brenda Major regarding positive stereotyping, self-stereotyping, discrimination and domination. Next, I will argue, using sociologist Pierre Bourdieu’s concept of habitus, both the Working Class Promise and the American Dream are structured and structuring social concepts which reinforce discrimination and domination through exclusion. In line with Bourdieu, I will use social philosopher Louis Althusser’s arguments to show how the Working Class Promise and the American Dream reproduce the roles of the social hierarchy and domination. I will support this demonstration with evidence from McCoy and Major’s study showing how various forms of discrimination are reproduced in society when individuals believe in the American Dream. Having accepted the social insights of these scholars, I would propose, based on the arguments of philosopher Immanuel Kant regarding the intrinsic worth of rational beings and contrary to elitist or socialist views, the solution is for Americans to reject these ideologies.