2013 Abstracts

Brook Owen, Dixie State University Theatre My theory is that theatre can portray emotion and convey a story without verbal communication. As an actor, I struggled to portray emotion while using a memorized script. As I worked through my theatrical training, I was introduced to exercises that took away language. I found artistic freedom in these exercises and formulated a research project to understand what would happen if actors were given freedom from language completely. This research is founded in the works of artists such as mime John Weaver and absurdist Samuel Beckett. One of the most prominent movement artists of our time is Anne Bogart. Her movement technique, called Viewpoints, focuses on the physical aspects of portraying emotion and kinesthetic response. This is where my physical research started. In rehearsal, actors experiment with Bogart’s viewpoints, while responding to different styles of music. Then I begin to move away from Bogart’s research. While allowing them to maintain their own organic choices, I give my actors more specific acting objectives with which to work. For example, “Imagine that your character wants to purpose.” Once completed, the actors talk to me about how the exercise expanded their physical toolbox. One comment from an actor symbolizes the heart of this research. “When the music starts, you think of an emotion, and then you start responding to that emotion. That’s when the character starts to form. And the next thing you know, you have a little story around it.” Repetition of this process will culminate in a narrative movement piece.

Jennifer Sumsion, Utah Valley University Art I am influenced by natural elements, the combination of shapes, colors and textures are what interest me. The commonplace, banal and unnoticed aspects of nature draws my focus and inspires me capture images to my vision and transform them onto paper and canvas. The constant change in rocks, leaves, skies, trees and water continue to focus my attention and are reflected back into my images. The balance of lines, colors and tones adds patterns energy and new life when mixed together. Nature can look foreign when viewed close-up, intensifying the smallest detail. Unnatural influence on the environment has added a unique aspect to nature. It can create extraordinary patterns and encourage new images that are beautiful in themselves. I enjoy the way leaves fall on a sidewalk, the patterns of melted water and salt on the road after a snowstorm, the ice crystals that form on tree limbs when pollution levels are high and the light and shapes reflected in shoveled snow when it begins to melt. I use cool and warm colors together to create a bounce off of each other. I enjoy using oil paints, ink charcoal, acrylic, and nupastels in my work.

Thomas Skousen, Dixie State University Art Concordia apostolorum, the union of apostles, is found in literature, art, and even the topography of Rome. Rome is unique in the fact that it unites Peter and Paul. Early Christians revered these saints and dedicated two of the most important churches in the city to them. Their deaths in Rome were a crucial factor in identifying Rome as the principal capital of Christendom. As Christianity spread and was accepted throughout the empire, different Christian centers arose, aside from Jerusalem and Rome. In order to maintain its place at the top tier of the Christian hierarchy, the Roman Popes used the idea of concordia apostolorum to not only speak to Roman citizens, but also to pilgrims who visited the city. The use of concordia apostolorum extended into the ninth century with Pope Paschal I’s (817-824) mosaics at Santa Prassede. These have historically been categorized into the Carolingian Renaissance. However, Paschal and earlier popes were trying to create a uniquely Roman Renaissance, and Peter and Paul’s place in art is crucial to their propaganda. The apse mosaic in Santa Prassede employs concordia apostolorum to promote the primacy of Rome. Louis the Pious inherited the empire from his father Charlemagne and surrounded himself with bishops and scholars who questioned the authority of the pope, thus igniting a struggle between the pope and the Carolingians. This paper will show that Paschal I commissioned art that emphasized his role in carrying out the ministerial work of Peter and Paul. In art and in life, Paschal sought to become not only the principal figure in Christianity, a living apostle, but also a chief player in Europe’s political structure.

Krista Brown, Weber State University Interior Design Research has shown that through January 2007, the wounded-to-fatality counts of Operation Iraqi Freedom is higher (about 7.6) than during earlier U.S. military conflicts, such as the ratio of 5.2 for Vietnam (Goldberg, 2010). This means that more soldiers are dying more from hostile combat rather than sickness or non-battle injuries. The Salt Lake area is not currently set up to accommodate the needs of these returning military personnel who have been inflicted with a war injury. Rooms are not adequate and not all necessary areas such as burn rehabilitation are addressed. The government has allowed for a veteran support home to be put into an existing building in Salt Lake City, UT. The objective of the veteran’s support home, “Hope Against Hope,” is to create a facility approximately 15,000 square feet that creates an area for veterans to heal and adapt to their new life style. Currently there is a lack of facilities in Utah that can fulfill all of the needs the “Hope Against Hope Veteran Support Home,” plans to accommodate. This support home will be located in Salt Lake City near the University of Utah hospital. This area was chosen because of the close proximity to the airport, the local medical support and emergency services, as well as the beauty of the surrounding area. Among the needs for the “Hope Against Hope,” center include future expansion, comfort, and should reflect universal design principles. In addition day lighting principles will be a focus. Research has shown that people in institutional areas don’t receive adequate exposure to natural light needed to synchronize sleeping patterns (Brawley, 2009). An advantage of the current location of the center is the close proximity to the Wasatch Mountain Range which provides spectacular views. This advantage will be used to promote healing as well as giving patients a sense of night and day in a home like setting. Color, common rooms, and a flowing floor plan will also provide a new life and hope for injured veterans as they recover from injuries sustained in military service.

Kyra Marie Bell, Weber State University Interior Design The negative impacts that tourists can have on the environment is a major reason that so many pristine natural habitats are off limits to travelers. With so many tourists around the world, the effects of carbon emissions from transportation, excessive waste, and harm to delicate ecosystems, these restricted areas seem to be growing (Center for Ecotourism and Sustainable Development (CESD), 2009) As a result, ecotourism has started to gain popularity, as it focuses on minimizing ecological and socio-cultural impacts (TIES, 2012). With the emergence of LEED certification, builders and designers are now provided with a way to implement green building design throughout the entire building process (USGBC, 2011). This makes it possible to not only construct a hotel built in sustainable ways, but to continually have a lower impact on the environment and educate people on ways they can lessen their own footprint. However, less than 100 hotels world-wide have achieved LEED certification, and as of 2010 there is only one such hotel in Utah (HKS, 2011). In Southern Utah, where many of the states national parks are located, lodging that meets the needs of ecotourism by limiting its impact on the environment is nearly non-existent. In order to visit the beautiful, undisturbed environments available in these regions, tourists need a place where they can stay that they know focuses on sustainability and limited impact on the environment as much as the tourists do. Having an ecotourism hotel in Southern Utah would encourage “tourism that seeks to minimize ecological and socio-cultural impacts while providing economic benefits to local communities” (TIES, 2012).In order to meet these needs, The Hotel Viridi will be built as a 15,000 square foot eco-tourism hotel in Southern Utah that meets all the LEED certification requirements as well as the 12 Aims of Sustainable Tourism (Center for Ecotourism and Sustainable Development (CESD), 2009). These needs will be met through the use of an adaptive re-use building, in which the hotel will incorporate four different aspects of design; hospitality design as found in all public areas of the hotel, a residential area for hotel guests to stay, healthcare design in the form of an onsite gym, and commercial design where the offices and lecture hall are located.

Sarah Breinholt, Weber State University Interior Design Spiriti Forti Rebuild and Recovery Center is a facility that heals the psychological and body issues for individuals with eating disorders. Research has shown that long-term stay care facilities should contain a “home-like” atmosphere that reflects the site’s surroundings. In a study by Schneider (2008) explains the importance for the patient to feel comfortable and relaxed in order to achieve maximum recovery. In addition, a study by Kaya (2009) explores the effect that color has on individual’s emotions and health. A study that was completed on a group of individuals of all ages and all nationalities shows that overall; people have very similar reactions to the same colors in spite of age or race. The colors blue and green have been recognized to reduce anxiety and promote calmness and well-being. Because of this reason, this color palette will be implemented in the design of this recovery center. A former historic train station in St. George, Utah has been acquired by Spiriti Forti Rebuild and Recovery Center to be renovated to accommodate a getaway for patients to escape the world and rebuild their body image and self-esteem. Among the features of the care center will cafeteria/kitchen that focuses on education and nutrition, rooms for long-term stay, as well as spaces for multiple activities for individual and group building exercises. This facility should become a place that provides all the necessary components for patients to make a full recovery of their psychological and image disorders.

Hilary Wolfley, Brigham Young University Dance The purpose of this project is to enhance the caliber and reputation of ballet in higher education-specifically contemporary ballet at BYU-while strengthening my own choreographic abilities under the guidance and coaching of my mentor, Shani Robison, Associate Professor. I have choreographed an original contemporary ballet entitled “Transition” that explores the ways in which we react to the challenges of change in our lives. Through exploring this theme, creating innovative movement, and rehearsing with talented dancers, I am working closely with my mentor to fulfill UCUR’s mission to creatively contribute to the discipline of contemporary ballet. I recently completed this project as the culmination of my undergraduate work at BYU as a dance major. This is the link to my project as performed last week at the BYU Ballet Showcase: http://youtu.be/j1t0MPcLUyw (DVD available upon request). The BYU Ballet Program has selected me as the only ballet student from BYU this year to represent undergraduate student creative work by presenting my project at the American College Dance Festival Northwest Region at Southern Utah University March 11-15, 2013 (ACDF). During this festival, my piece will be viewed by collegiate dance students and educators from across the country and adjudicated by internationally renowned experts in the dance field.

Brittany Brown, Weber State University Interior Design According to the article Contributions of Built Environment 68% of the adult population is considered obese. (Rahman, 2011) The article also states that childhood obesity is on a steady rise. The research provides evidence that obesity shortens Americans life expectancy by 2 to 5 years on average. The study by Rahman recommends that adults engage in at least 150 minutes of moderate-intensity aerobic activity weekly and at least 60 minutes every day. The Spencer Weight Loss Retreat is a 18,000 square foot facility located in Meridian, Mississippi. The weight loss retreat will provide weight loss training and medical attention for those residents of Meridian who are considered obese and want treatment to gain back their lives. The weight loss center will provide each patient with a personal trainer who will guide in their weight loss. The facility will provide the patients access to their gym where they can use treadmills, bikes, weights, outdoor routines, and other activities to meet the 150 minute workouts a week. The Spencer Weight Loss Retreat will provide a classroom to educate the patients in nutrition and other life skills.

Machaela Burt, Utah State University Theatre Arts This research study explores the efficacy of the “Living Literature Project”, a program that teaches traditional English curriculum in high school classrooms using theatre-based strategies to enhance understanding of Shakespearean texts. The study was piloted with two control classes and two intervention classes in 2012 with strategies such as viewing performances by experienced actors during class and paraphrasing texts into modern speech to assess the impact on learning through the use of teacher-administered pre and post project surveys assessing student attitudes towards Shakespeare as well as content quizzes. The results of the pilot study suggested that students who participated in the project achieved higher quiz scores, but their attitudes about Shakespeare were not changed. The pilot study informed further developments of the teaching strategies and led to further curriculum development. The project is currently in session for the 12-13 school year. The study is being expanded to include a larger number of classes that will also assess teacher satisfaction with the academic progress made by the students involved. The results will be assessed through revised surveys and quizzes as well as focus groups with the students. Results are anticipated in April 2013. The pilot study suggested that arts integration and the building of cross-curricular relationships have a positive effect on student learning. The current study hopes to reinforce these results with more accurate assessments and to see increased topical interest, measured through focus groups.

Natalie Batty, Westminster College Biology Hydrocarbon metabolizing microbes play a critical role in bioremediation and bio-augmentation projects. In 1978, Brock et al. identified microbes in the Great Salt Lake (GSL) capable of metabolizing hydrocarbons, but since then these microbes have gone unstudied. This research has isolated and identified bacterial species capable of hydrocarbon metabolism from GSL. Though capable of survival in the high salt concentration of the Great Salt North Arm, we have demonstrated that these species are also capable of thriving in low salt concentrations. This research will continue with the characterization process to identify what hydrocarbon sources each species is capable of degrading.

Merima Beganovic, Westminster College Molecular Biology Nuclear Pore Complexes (NPC) create aqueous channels embedded in the nuclear envelope and are made from a network of proteins called nucleoporins (nups). The nucleoporin Nup153 has previously been found to be required for proper assembly of the nuclear lamina. In this study, a fragment of Nup153 was overexpressed in order to impede Nup153 function in T-Rex HeLa cells. After inducing the expression of the dominant negative fragment, I examined the localization of lamins A, B1 and B2, as well as SUN1, Emerin, and BiP, three proteins that mark cell membrane compartments. Lamins B1, B2, SUN1, and Emerin were found to mislocalize to the cytoplasm of the cells, and colocalization among the proteins was observed. Lamin A also had an abnormal phenotype unlike that seen with the B lamins, but indicative of a problem with integration of Lamin A into the nuclear lamina. Colocalization of the various lamin isoforms with membrane proteins such as SUN1 and Emerin indicates a problem with membrane assembly. The distinct localization of BiP, however, suggests that there is a “nuclear-like” membrane in the cytoplasm that either does not incorporate normally into the nuclear envelope as it forms or is newly-recruited to mislocalize the lamin proteins.

Taylor Nelson, Weber State University Botany Arnica is a common form of complementary medicine used to treat bruises and sore muscles. Most arnica preparations are made as an oil or gel by soaking the flowers of Arnica montana (a member of the Asteraceae) in an oil, such as olive oil, to extract helenalin, the presumed active component. Helenalin, a sesquiterpene lactone, has been shown to inhibit transcription factor NF-?B, a factor which controls the expression of dozens of genes involved in inflammation responses (Rungeler et al. 1999). Due to arnica’s popularity, A. montana is becoming scarce in Europe (its native range) and is sometimes substituted commercially with A. chamissonis (Cassells et al. 1999). Therefore, this study focused on finding other sources of helenalin among the Asteraceae. A. chamissonis, A. cordifolia, A. latifolia, A. longifolia, A. mollis and Helianthella uniflora were collected from Alta, UT, and Helenium autumnale from Salt Lake City, UT, at elevations of approximately 10,500 feet and 4,200 feet, respectively. Sesquiterpenes were extracted from the flowers by dipping them in methylene chloride and separated by thin-layer chromatography. Helenalin and possible helenalin derivatives were identified by their reaction with vanillin and comparison to a pure helenalin standard. Flowers from all seven of the species contained helenalin, with Helenium autumnale containing the highest concentration and A. latifolia the lowest. Therefore, all of the species tested in this study have the potential to be used commercially to prepare arnica salves and thus allow the A. montana population to re-establish itself. An additional finding was that A. chamissonis grew the best under greenhouse conditions, which may explain its substitution in some commercial salves.

Scott Nagao, Weber State University Microbiology Batrachochytrium dendrobatidis belongs to the fungal phylum Chytridiomycota (chytrids), and in 1999, was both identified as a new species and associated with causing chytridiomycosis in amphibians. Since its identification, this fungus has been implicated in rapid population declines in multiple amphibian species in North and Central America, Europe, and Australia. In adult amphibians, the fungus colonizes only the keratinized skin cells on the ventral and dorsal surfaces as well as on the webbing between the toes. Infection is associated with hyperkeratosis resulting in disruption of the skin’s osmoregulatory function, dehydration, electrolyte imbalance and death. There is data that indicates some bacteria that live on the epidermis of amphibians produce chemicals that inhibit the growth of B. dendrobatidis. However, this data is associated only with salamanders. There is no similar inhibitor data published for frog and toad species, and no data published on the identification of normal skin biota of frogs and toads. We have isolated over 175 bacterial isolates from 67 toads and frogs, extracted DNA, and used PCR to amplify the 16S ribosomal DNA gene. Amplicons have been sequenced to determine speciation based on genetics. This is the first report of normal bacterial skin biota in frogs. Further, a skin swab was also taken from these frogs and used to analyze which are infected with B. dendrobatidis via quantitative PCR screening. While the prevalence of fungal infection appears to be low, we have been able to correlate fungal-positive hosts with number and/or species of normal bacterial skin biota.

Heather Reynolds, Westminster College Biology The presence of mercury in a food chain can have harmful effects, including altering behavior, on organisms. Mercury is typically found in aquatic ecosystems, however recently is also recognized as a potential problem in terrestrial ecosystems. High levels of mercury have been found in the Great Salt Lake in a form able to bioaccumulate up the food chain. If the aquatic ecosystem is linked to the terrestrial ecosystem, then some of the highest predators included in this food chain may be songbirds that eat spiders. We quantified mercury in the blood of the Loggerhead Shrike, Lanius ludoviciaus, on Antelope Island. Upon being caught, the birds were measured, color banded, and a blood and feather sample taken from each of the 15 shrikes to be tested for mercury. Mercury concentrations ranged from 0.96 to 4.00 ppm, with a mean 1.14+/0.31 ppm. Sub-lethal effects in songbirds from another study were seen with concentrations ranging from 2.0-3.5 ppm. Two shrikes we tested exceeded that range. There was high variability in concentrations of mercury, which might be related to distance from shore. These preliminary data demonstrate that some mercury from the aquatic ecosystem is bioaccumulating in the terrestrial ecosystem on Antelope Island, and there may be harmful levels in the birds which could lead to change in behavior, and eventually decline in population. Future studies will investigate the costs of mercury contamination.

Chelsie Thomas, Weber State University Zoology The invertebrate ribbon worm, Paranemertes peregrina, serves as a unique model in that it thrives in an environment exposed to drastic fluctuations in salinity during tidal interchange. Its ability to sustain homeostatic integrity is not well understood. With an aim to clarify this phenomenon, total RNA was isolated, and reverse transcription with polymerase chain reaction allowed us to serendipitously clone and elucidate a 488 base pair region of a gene coding for a Tachykinin Receptor (TKR), a subtype of G-protein coupled receptor (GPCR). This region shows 79% homology to the mouse TKR-2 mRNA sequence, and 44% homology to human Neuromedin-K receptor’s amino acid sequence. Specifically, we have isolated a portion containing a cytosolic carboxy-terminus that has classically been associated with palmitoylation or otherwise hydrophibicity-enhancing interactions. This process facilitates the docking of cytosolic subunits to the membrane in the assembly of GPCRs, acting as a regulatory component. In vertebrate models, much of these proteins are conserved. Given that only a few of these genes have been reported for invertebrates, this suggests a critical need for investigation of the evolution of TKRs as they relate to stress response. Additionally, this receptor poses questions about its potential role in pain, with the prospect of revealing insight about the long-elusive pain perception in invertebrates.

Morgan Anderson, Westminster College Environmental Studies Emigration Creek, part of the Salt Lake City watershed, runs through Westminster campus at two points: by Garfield school and through main campus. We assessed several measures of water quality, to understand the impacts of anthropogenic activities and urbanization on this stream ecosystem. Additionally, we identified areas for restoration with the ultimate goal of reintroducing the endangered native Bonneville Cutthroat trout. Beginning in June 2012, we monitored the creek monthly, and will continue for a full year’s time. We assessed the structure and stability of the stream along with dissolved oxygen, pH, temperature, and turbidity. The macroinvertebrate community composition was analyzed also as a biological indicator of stream health. Along both reaches lack of vegetative cover, lack of large rocks to support the lower banks, and scouring and deposition along the steam bottom were common. We found that for all water quality measures monthly average levels were in acceptable limits for trout populations except for dissolved oxygen. Dissolved oxygen dropped below optimal levels in July and August, most likely due to an increase in water temperature. We found that both reaches have moderate species diversity of macroinvertebrates, though the community is dominated by species that thrive in water highly polluted with organic waste. Preliminary results suggest that the current condition of Emigration Creek is not optimal for cutthroat trout, largely due to seasonally low dissolved oxygen levels and lack of preferred food. Continued monitoring, along with a comparative study of nearby creeks, will further our understanding of the impacts of urbanization and the restoration efforts needed to support native fish populations in the Salt Lake Valley.

Alisha Ryan, Weber State University Microbiology One of the major challenges facing U.S. military caregivers is the presence of multidrug resistant organisms in extremity wounds. The most frequently identified drug resistant strains of bacteria found in these wounds are Acinetobacter baumanni, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus. Due to these organisms rapid increase in resistance to the commonly used drugs, it is crucial to discover and establish alternative methods for treating these microbial infections. Antibiotics are currently the most common treatment for infections by these pathogens, and there is little data on the evaluation of phytochemicals as potential chemotherapeutic agents that could take their place. We have screened 24 individual compounds from 9 major compound families to determine if plant-based phytochemicals could be explored further for use in treating bacterial infections in patients with military wounds. All compounds were tested to determine the minimum inhibitory concentration (MIC) and minimum lethal concentration (MLC). Of the 24 compounds tested, 21 (87.5%) inhibited at least one strain used in this study, with only 1 of the 24 (4.2%) inhibiting all strains. There were 10 (41.7%) of the compounds that displayed MIC values less than 100 _g/ml. For compounds displaying MLCs, they ranged from 2.5 mg/ml to 78.13 g/ml. While there is much more research that needs to be done with each of these compounds, this work is a crucial first step in the drug discovery process. We believe that several of these may serve as potential novel inhibitors of these drug-resistant bacteria.

Brooke Nelson, Weber State University Interior Design According to the National Substance Abuse Index (2006), Utah women account for 31.7 % of the entire drug abusing population. Mind-altering substance abuse among women creates many unique problems in their lives according to Wesa and Culliton (2004). Additional research by Wesa and Cullliton shows this can affect their futures, finances, families, health, and relationships. Many of the women have other underlying problems that lead them to use mind altering substances to cope with these problems as researched by James (2011). In the Ogden, Utah area, mind-altering substances have created an epidemic that needs to be helped. Serenity, A Drug Abuse Recovery Center, will be an 18,000 square foot building located in Ogden, Utah. Serenity helps facilitate mental and physical healing to create an overall wellbeing for the women. Research has said that women are more likely to receive help for mental conditions than the substance according to Greenfield (2006). Mental disorders and substance abuse will be addressed at the recovery center. Individual and group therapy methods will be used because of their effectiveness shown by Greenfield. Research by Greenfield has shown that women in treatment centers have a greater chance of retention if dependent children are present. Serenity will provide a private room for a woman and two dependent children to stay at the center. The center will include nutrition education and dining area, and a children’s area. The children’s area will have reading, playing, learning, and therapy treating areas.

Jackie Parker, Weber State University Botany Forensic researchers as well as law enforcement officials rely heavily on herbarium type specimens for field investigation. An electronic catalog could greatly assist forensic professionals by providing easy access to area specific specimens. The goal of this project was to compile a database and dichotomous key of native plants found in Weber County, Utah. Pollen and woody material resist degradation, making them perfect candidates for forensic analysis. Using the USDA plant database, all native flowering plants were identified (729 in total). Plant pressings, microscope slides, digital micrographs and line drawings were made of two-hundred native species to date. Pollen and woody tissue were gathered from representative plants and prepared for permanent herbarium collection. Woody tissue and pollen preparation had previously been optimized for best imaging results. Rudimentary dichotomous keys for both woody tissue and pollen were made using micrographs and line drawings to illustrate distinguishing features between plant species. As new species are prepared, they are included in the dichotomous key. The primary purpose of this collection is for it to be used by forensic investigators with limited botanical training. In the spring of 2013, field tests will be conducted to determine how effective the dichotomous key is when identifying unknown specimens. After the layout is finalized, the key will be presented to local law officials as an additional tool in the forensic repertoire.

Jennifer Jorgenson, Weber State University Microbiology Enterococcus, a bacterial genus that normally inhabits the gastrointestinal tract of animals, can be pathogenic to humans, causing urinary tract infections, sepsis and other serious diseases. It is also one of the major causes of hospital acquired infections. One important complication of those infected with Enterococcus is the fact that they often have a high level of antibiotic resistance, making effective treatment of patients more difficult. While it is a normal inhabitant of the gastrointestinal tract, it can survive outside its host in the environment, even in adverse conditions, such as the Great Salt Lake. In this experiment, isolates of Enterococcus were collected from the Great Salt Lake and from fresh water sources. These isolates were tested for different phenotypic characteristics and for their antibiotic resistant patterns against five antibiotics. The results of the Kirby Bauer disk-diffusion assay demonstrated that 47% of enterococcal isolates from the Great Salt Lake were resistant to one or more of the five antibiotics. In contrast only 15% of Enterococcus isolated from the fresh water source were resistant to one or more of the five antibiotics. This has implications for those who have recreational and occupational contact with the Great Salt Lake.

Lauren Johnson, Weber State University Microbiology Bacteriophages in aquatic environments play a significant role in bacteria population control, as well as recycling nutrients. The bacterial genus Halomonas is commonly found in the Great Salt Lake (GSL), but very little is known concerning its population dynamics. This euryhalophilic genus is highly versatile concerning its ability to grow in a wide range of substrates and environmental conditions including salt concentration. To better understand GSL microbial ecology, seven strains of Halomonas were isolated from the GSL and identified using 16S rRNA. Samples of South Arm GSL water were filtered twice through a 0.2 m filter, and tested against these Halomonas strains using soft agar overlays to detect Halomonas phage. Three strains exhibited plaque formation indicating the presence of phage. Halomonas phage isolates produced very small plaques, sometimes barely visible. Individual phages were isolated by vortexing agar plugs taken from single plaques in sterile saline then filtering through a 0.2 m filter. From host range streak plates, a single phage isolate (LJ17) appears to infect 4 closely related Halomonas strains. Electron micrographs of LJ17 phage indicate it has a small icosahedral head and perhaps a very short tail. There also appears to be a satellite phage that may be associated with LJ17. There are no reports of Halomonas phage isolated from the Great Salt Lake (GSL), although there are phages found for marine Halomonas strains. Successful isolation and characterization of novel GSL Halomonas phage, besides being critical for development of host/phage models, will also allow studies of GSL microbial ecology.

Graham Doherty, Westminster College Biology The Great Salt Lake is home to Artemia Franciscana, a species otherwise known as brine shrimp. These halophiles are able to live in extreme environments that have higher than average salt concentrations. Brine shrimp live their entire life cycles in the Great Salt Lake and can be found in both the north and south arm of the lake. Currently, the genetic information regarding the brine shrimp populations at different locations in the lake is underdeveloped. The Artemia populations live in different microenvironments throughout the lake with different physical characteristics and barriers between one another. The physical environment also affects the salt concentration in each microenvironment. Other studies have shown that the increased salinity of an environment accelerates the rate of change in the mitochondrial genome. We feel that different salinity levels in each microenvironment will create different amounts of genetic variation. Our goal is to determine if the distribution of genetic variation is different at different sites in the Great Salt Lake Artemia population.

Dakota Hawkins, Westminster College Arts and Sciences Abstract. Previous studies have documented effects of urbanization on the behavior, reproduction and survival of wildlife. Specifically, noise pollution in urban areas has been known to mask communication among several avian species. In a previous study in Mexico City, House Finches increased the frequency (pitch) of their songs to help mitigate the effects of low frequency urban noise. To document the average minimum frequency of House Finch song in Utah, we recorded House Finches singing from May 2012 to August 2012. Three sample sites with 1 km radii were established in Salt Lake City, Utah while a fourth site was sampled in Logan, Utah. Ambient sound was recorded at locations where songs were recorded to measure urban noise. Average minimum song frequencies and ambient noise were calculated for three sites. Frequency measurements were not significantly different among the three urban populations. Future studies will compare the minimum frequency of these urban populations to nonurban populations and investigate syllable structure and use.

Jeff Collins, Westminster College Biology The Great Salt Lake (GSL) has some of the highest mercury concentrations ever measured in surface waters. The accumulation of high levels of mercury, particularly its more toxic and readily biomagnified form, methylmercury, has been recorded in many species that inhabit or feed directly in the GSL, including waterfowl. However, no studies at the GSL, and very few studies elsewhere, have investigated the transfer of mercury from aquatic ecosystems to terrestrial ecosystems. We hypothesized that brine flies are able to accumulate mercury during their larval stages in the lake, then transfer this mercury to adjacent terrestrial ecosystems when they become flying adults. Concentrations of total mercury (HgT) and methylmercury (MeHg) were measured in samples of surface water and brine flies (larvae, pupae, and adults) collected once each month between February and July at Antelope Island, in the GSL. Samples were collected from two distinct sites: one along Gilbert Bay on the west side of the Island, and the other along Farmington Bay on the east. On average, HgT concentrations are greater in water samples from Farmington Bay (19.3 ± 9.0 ng/L unfiltered; 13.5 ± 10.1 ng/L filtered) compared to Gilbert Bay (8.5 ± 7.5 ng/L unfiltered; 9.2 ± 9.6 ng/L filtered). MeHg and HgT levels in brine flies displayed the opposite trend, with higher levels at Gilbert Bay (567 ± 123 ppb HgT; 425 ± 12 ppb MeHg) than at Farmington Bay (270 ± 60 ppb HgT; 208 ± 35 ppb MeHg). Potential explanations for the trends observed will be discussed.

Kristie Williamson, Weber State University Athletic Training Context: Cranial Nerve V (CN V; Trigeminal nerve) is responsible for facial sensation. CN V has three separate branches which include ophthalmic, maxillary and mandibular. The ophthalmic nerve (V1) carries sensory information from the forehead; the maxillary nerve (V2) carries information from the cheek; and the mandibular nerve (V3) carries information from the chin. It is important to test all three areas of cranial nerve V to accurately assess full nerve function. Previous research has indicated that pressure threshold is not affected by age, however, normative values for two-point discrimination for CN V have not yet been established and may prove useful in concussion evaluation. Objective: Initial investigation aimed at determining gender-specific normative values for CN V two-point discrimination. Design: Prospective repeated-measures design. Setting: This study was performed in the athletic training facilities on the campus of a large Division I institution. Patients or Other Participants: Convenience sample of 106 healthy students enrolled at our institution (43 females, average age: 23.2; 63 males, average age: 22.1). Participants had no history of concussion within the last year, no facial scaring or plastic surgery and not a current smoker. Interventions: Measurement was taken using a two-point discriminator (Disk-Criminator) on the mid-forehead, mid-cheek, and mid-chin. Participants closed their eyes while each measurement was taken. Light pressure was applied (force equal to approximately 10-15 grams to produce blanching of the skin). Measurements were taken three times on each of three facial locations, sequentially. The smallest number in millimeters (mm) to correctly discriminate one/two points was recorded. The two pressure points were applied at exactly the same time. Main Outcome Measure: Discrimination score for each test location (mid-forehead, mid-cheek, mid-chin). Nested average was calculated within subjects for each test location; average was calculated between subjects for each test location. One-way between-subjects ANOVAs were conducted to evaluate potential differences in gender for each test location. Results: Between-subjects average for mid-forehead was 7.8mm (+/2.9), for mid-cheek was 10.4mm (+/2.6), and for mid-chin was 5.9mm (+/1.9). There was a significant effect for gender for cheek normative value (p<0.01); average discrimination score for males was 11mm, average for female was 9.7mm.

Bryce Anderson, Brigham Young University Microbiology and Molecular Biology Antigen presenting cells digest and display peptides from foreign and infected cells on the major histocompatibility complex (MHC) that are recognized by T-cells through their T cell receptor (TCR). The affinity of TCR:peptide-MHC interactions has been shown to be low however, and in order to effectively use a soluble TCR for therapeutics we need to engineer TCRs with increased affinity. To do this, we have designed a single chain TCR (ValphaVbeta) called LLO118 that is specific for a naturally occurring Listeria monocytogenes epitope. Using yeast display, stable mutants that expressed the LLO118 scTCR at higher levels than the wild type on the surface of yeast were isolated and sequenced. In order to improve affinity of LLO118 we are mutating amino acid residues in the complementarity determining regions, sites important for the TCR to bind with the peptide-MHC. We are generating unique libraries of yeast cells with TCRs that have potential affinity mutations and using fluorescently labeled peptide-MHC tetramers to select cells that have TCRs with higher affinity. By repeating this process with the cells that have higher affinity we are working to get a TCR that binds with much higher affinity than the wild type TCR. These high affinity TCRs are promising for further research in connecting them to a cytokine, greatly reducing systemic damage and other complications caused by administration of this cytokine throughout the body. Thus, our goal is to design a high affinity TCR fused to a cytokine that can be tested for therapeutic use in targeting specific cells in the immune response and improving T cell memory.

Brad Ackerson, Brigham Young University Neuroscience The highjacking by alcohol and drugs of abuse of the mesocortico-limbic system in the brain is responsible for addiction, specifically the ventral tegmental area (VTA) and its projecting dopaminergic neurons to the nucleus accumbens (NAc). Over the course of addiction, a hedonic response is developed from lower than normal levels of dopamine (DA) in which the individual pursues drug-seeking behavior. The current accepted treatment methods for addiction are replacement drug therapies, group therapy, or individual counseling – the prior being associated with additional side-effects and an inability to overcome the hedonic response of the addiction. The aim of this study was to evaluate alternative and natural therapeutics that produce long-term potentiation (LTP) of the neuronal systems involved in order to overcome addiction with minimal to no side-effects. Using fast-scan cyclic voltammetry (FSCV), the effects of baicalein, a flavonoid isolated from the root of Sculletaria Baicalensis, and low-level laser therapy (LLLT) on DA release in the NAc core were evaluated in vitro and in vivo in Wistar rats. Local stimulation evoked in vitro demonstrated that baicalein administration (10, 50, 100 uM) 30 minutes prior to 80 mM ethanol attenuated the DA inhibition of ethanol. DA signals were evoked in vivo in the core of the NAc by electrical stimulation of the medial forebrain bundle (MFB) at the level of the lateral hypothalamus (60 Hz, 60 pulses) in isoflurane anesthetized rats. Both the intraperitoneal (IP) administration of baicalein (1.0 mg/kg) and the administration of LLLT (25 Hz, 630 nm) 30 minutes prior to ethanol (2.0 g/kg) administration IP attenuated the DA inhibition of ethanol. These findings suggest that baicalein and LLLT may prove as effective clinical therapies for addiction.

Adam Olson, Brigham Young University Plant and Wildlife Sciences Forests systems of the Central Rocky Mountains rely on an intricate balance of natural disturbance cycles in order to develop properly. Forest fires are one such disturbance, however, certain fire characteristics, particularly fire severity, can vary widely across forest landscapes. In our study, we examined the influence of fire severity on aspen regeneration as well as aspen defense against wildlife and livestock browsing. Our results indicate that high or moderate burn severity is more favorable to aspen regeneration and survival. These areas of severe burn result in a higher density of aspen suckers, more vertical and lateral growth, greater chemical defense concentrations, and less browse damage than the neighboring plots of low or no burn. This data suggests that fire severity should be taken into account when considering plant regeneration and susceptibility to browse damage in burned landscapes.

Michael McEntire, Brigham Young University Biology Female mate choice (intersexual selection) and male dominance interactions (intrasexual selection) can each play important roles in sexual selection. These two mechanisms tend to be discussed in isolation. The goal of this study is to explore the interaction between these two forms of sexual selection. To test this idea, we focused on the livebearing tropical fish system Poecilia gillii. We grouped males into similarly sized pairs and observed them for a week to determine which male was dominant. These pairs were then presented to females in mate choice trials to ascertain female preference. We also photo- graphed the males to determine coloration. We found that females were unable to detect dominant males without viewing the physical contest and that carotenoid coloration bore no effect on female preference. Females tended to choose the male to their left, suggesting the preferential use of their right eyes in making decisions on mate choice.

Ji Su Park, Brigham Young University Nutrition, Dietetics, and Food Science Selenium (Se) and soy have each been shown to reduce risk for prostate cancer when consumed at high levels. The purpose of this project was to define the molecular mechanisms of prostate cancer chemoprevention by Se and soy, and to describe how timing of dietary treatment modifies those effects. [C57BL/6 X FVB] F1 TRAMP (TRansgenic Adenocarcinoma of Mouse Prostate) male mice were fed stock diets high or low in soy, with or without a supplement of Se (4.0 mg Se/kg BW as Se-meth- ylselenocysteine) by gavage 5 d/wk in a 2 X 2 factorial design. Mice were exposed to different diets starting from conception, 6 weeks, or 12 weeks of age and were sacrificed at 18 weeks. Three-way ANOVA showed that supplemental Se increased serum and liver Se, with significant interactions with both time and soy intake. Selenium dosing decreased BW independent of soy intake and time of dietary intervention. Both Se and soy decreased epididymal fat pad weights, with Se’s effects being more pronounced in mice exposed to diets from conception than from 6 wk. Urogenital tract weights, a measure of prostate proliferation and tumor volume, were significantly reduced by Se supplementation (P<0.001) and soy (p=0.044), independent of time of dietary intervention. Histological examination of mouse prostates is in progress to determine dietary effects on disease progression. These data suggest that, in this model, chemopreventive efficacy of Se and soy does not differ between prenatal and early post-natal introduction.

Whitney Hoopes, Brigham Young University Microbiology and Molecular Biology HspB2 is one of eleven known small Heat Shock Proteins (sHSP) that is expressed in human heart and skeletal muscle. In response to cellular stress, heat shock proteins play a vital role to help misfolded proteins and proteins susceptible to denaturation maintain their structure. Two members of the sHSP family, CryAB and HspB2, are both required for normal heart function and cardiac muscle integrity. CryAB-deficient mice have defects in cardiac muscle structure whereas HspB2-deficient mice display energy deficits (Rajasekaran et al. 2007). The contrasting phenotypes of CryAB and HspB2 suggest differential roles for these molecular chaperones in the heart. HspB2 has been found to localize with the mitochondria in several different cell lines and overexpression of this sHSP has been shown to support survival of cells against heat stress (Nakagawa, 2001). To understand the role and mechanism of HspB2 in cardiac muscle energy regulation, we have used a yeast two-hybrid (Y2H) system to uncover the novel protein binding partners specific to HspB2. From screening a human heart cDNA library, HspB2 interacted with approximately 10,000 out of 20 million plasmids. We have sequenced more than 1000 of these putative interactors and have identified over 100 unique proteins. Over 40% of these protein partners are involved in mitochondrial energy production and another 25% in cardiac muscle structure maintenance. In addition, we have identified an interaction between HspB2 and the related sHSP CryAB. We then compared this data with mitochondrial HspB2 binding partners identified by mass spectroscopy (MS) through a large-scale bioinformatics analysis and constructed a protein-protein network. Y2H dependency tests were conducted to verify interactions identified by both Y2H and MS. Following yeast verification, a subset of the interactions were confirmed in C9H2 cardiac cells through coimmunopurification. Our research describes the first protein-protein interaction network for any sHSP, supports a role for HspB2 in mitochondrial energy production and suggests a link between mitochondrial energy production/redox stasis and stressed cardiac muscle maintenance.

Christian Boekweg, Brigham Young University Plant and Wildlife Sciences It has been shown that the population densities of deer and elk in the Rocky Mountains are at an unprecedented high. The heavy browsing of said species (ungulates) on regenerating aspen suckers can be devastating, leading to homogenously aged aspen stands that now have reduced resilience to drought, fire or logging disturbance, and an increased susceptibility to pathogens. Lastly, the extensive, highly selective herbivory of the aspen suckers may cause a shift in forest composition away from the aspen tree to an increase in other, less palatable species. We selected 186 sites across the 3 national forests of Central and Southern Utah, and characterized stand composition using the point quarter method. Stands were defined by successional stages from early to late; aspen, mixed or conifer. We then used pellet counts to estimate animal density and evaluated the effect of animal density and stand type on the regeneration success of aspen suckers. The key result of our study is that high ungulate density is highly correlated with reduced sucker heights (p<0.001). This indicates that high ungulate density impedes aspen regeneration by preventing aspen from recruiting into the overstory. Our study suggests that closer monitoring of the long term effects of herbivory on aspen development and regeneration is necessary to ensure vigorous aspen forests.

Michael Peterson, Brigham Young University Biology A person’s predisposition to Alzheimer’s Disease is known to be influenced by both genetic factors as well as environmental factors. One know environmental factor is that known to affect risk for disease is early parental death. The purpose of this research is to better understand the complex factors that influence the disease by analyzing the relationship between the environmental factor of early parental death with genetic variants known to influence the disease. We used extant data from the CCSMHA, an ongoing aging study including 89.7% (5092 of 5677) of all of the eligible residents of Cache County, Utah. This data includes information about environmental and psychosocial stressor of the subjects as well as information about physical examinations, metal screenings, and individuals’ genotypes at many loci that are known to be related to Alzheimers Disease. We used multivariate logistic regression to determine the effect of early parental death by SNP interactions on risk for AD. For the analysis we cleaned the data by removing SNPs less than a minor allele frequency of 0.01, a Hardy-Weinberg equilibrium value of 110-6, and a maximum missing snp call of 0.2. Individuals were also removed if genotyping rate was less than 0.2. After filtering we had 262 cases, 239 controls and 0 missing Final Results will be presented at the Conference.

Jesse Cobell, Brigham Young University Biology Alzheimer’s disease (AD) is the sixth major cause of death in the U.S. However, at present, no diagnostically useful serum markers for AD have been identified. Hence, we used a novel serum proteomic approach to interrogate the low molecular weight proteome for serum biomarkers. This allowed for survey of around 5000 low MW species. To reduce ion suppression, an acetonitrile precipitation step was used to remove high abundance serum proteins. Protein-depleted sera from 58 cases and 55 controls were analyzed by cLC-ESI-QTOFMS/MS using reverse phase chromatography. Data were reviewed using Applied Biosystem’s Analyst-QS software to compile spectra. Differentially expressed peptides (cases vs. controls) were analyzed statistically using the Student’s t-test. This led to discovery of 36 candidate biomarkers. Additionally, we compared AD subjects with more severe disease (Clinical Dementia Rating (CDR) =3) with non-demented individuals (CDR=0) and found 23 biomarkers. Furthermore, on comparison of mild and moderate stage AD individuals (CDR = 0.5, 1, 2) with those with severe disease (CDR = 3), we found 24 biomarkers. Some of these biomarkers appeared more prominent in one gender. We then fragmented several of these biomarkers on an LTQ-Orbitrap XL hybrid mass spectrometer and cLC-ESI-QTOF-MS/ MS system using collision-induced dissociation to determine amino acid sequence analysis. We have identified 5 biomarkers and are in the process of identifying the remaining biomarker species. This serum proteomics approach found statistically different peptide abundances in subjects with AD. Additional biostatistical evaluations are underway to determine sensitivity and specificity of individual biomarkers and their combinations. Future studies will assess biomarkers according to disease stage and validate current biomarkers in blinded comparisons of other AD sera. This serum proteomics approach appears promising in locating and identifying clinically useful serum biomarkers of AD.

Rachel Perry, Brigham Young University Life Sciences Previous studies have indicated that Visinin-like protein (VILIP) may be a powerful tool in predicting disease progression and guiding prognosis of Alzheimer’s disease (AD). Cerebral spinal fluid (CSF) was collected from hundreds of individuals with varying levels of AD. The CSF was then analyzed for levels of VILIP protein using Luminex technology. SNPs were genotyped using the Illumina OmniExpress chip. SNPs found to have a Hardy-Weinberg frequency less than 1×10-4 were not included, assuming that this variance was due to a genotyping error. SNPs and samples missing more than five percent of the data were also not included. Following the cleanup of the data, an association test using linear regression was performed. Covariates used in the analysis included age, gender, and covariates that accounted for population stratification (PC1 and PC2). Over one hundred SNPs were found with a p-value less than 1×10-5. The genomic inflation factor for the generated data was 1. One marker showed significance at the genome-wide level. We have identified a genetic marker that shows significant association with CSF VILIP levels. This finding may provide insight into genetic control of VILIP levels, which may be a useful in understanding the pathological processes involved in AD.

Matthew Schneider, Brigham Young University Physiology and Developmental Biology Alzheimer’s disease, the most common form of dementia, affects millions of people per year. Research has shown that Alzheimer’s affects the hippocampus brain region, which is involved in learning and memory. Understanding learning and memory functions is imperative to comprehending both healthy brain functions and Alzheimer’s disease. Many researchers seek to understand both the causes and treatments of the disease, but tangible information remains elusive. Studies thus far have shown that to encode memories, the brain must change neural synapses to either strengthen or weaken those pathways, a process known as synaptic plasticity. Using electrophysiology techniques on mouse hippocampal slices, this project will provide further insight on memory formation and regulation by imitating synaptic plasticity mechanisms. I will look at a specific cellular pathway involving the protein receptor GPR55, which has recently been shown to induce synaptic plasticity. By understanding how the GPR55 pathway functions, this research will contribute to the understanding and treatments of Alzheimer’s and other neurodegenerative diseases.

David Marriott, Brigham Young University Physiology and Developmental Biology Acute stress has been shown to decrease Long-Term Potentiation (LTP) in the CA1 region of the mouse hippocampus. Additionally, stressed animals show signs of anxiety and suffer decreases in spatial memory tasks such as object recognition and maze navigation. Conversely, exercise has been shown to increase spatial memory task performance in mice, attenuate anxiety-like behaviors and enhance neurogenesis and LTP in the dentate gyrus. While the effects of stress and exercise have been examined independently, there is currently a lack of experimental evidence that connects how stress and exercise, when experienced by the same animal, might modulate LTP in the CA1 region of the hippocampus. In our ongoing study, mice have been separated into a control group, a stress group (restraint and tail-shock), and an exercise + stress group where mice have voluntary access to a running wheel (for 30 days) before undergoing the stress protocol. We hypothesize that exercised animals will experience a protective effect against the reductions in CA1 LTP. In the stress only group, preliminary data shows a modest stress effect on LTP, yet we are learning that factors such as controllability of the stressor or the ability to develop coping mechanisms might potentially attenuate

Tara Hammond, Brigham Young University Microbiology and Molecular Biology Genetic diseases, including Alzheimer’s, cystic fibrosis, and many cancers, can be detrimental to individuals and their families. Gene therapy can possibly cure these diseases by inserting a correct copy of the gene into the chromosome, upregulating good genes, or downregulating the harmful gene. When DNA is packaged into a cell, it wraps around histones-an octamer made up of two tetramers, each containing four different subunits to create nucleosomes. Where the nucleosome sits on the DNA sequence determines whether or not a gene can be transcribed. In heterochromatin, nucleosomes are denser and DNA is tightly packed, thus causing genes to not be transcribed. Euchromatin contains looser packed nucleosomes and therefore has higher transcription levels. This project seeks to determine if modified nucleosomes have DNA sequence preferences. We are working with histone H3 to tri-methylate lysine 4, which has been shown to correlate with euchromatin. The modified histone will be used to create octamers. C. elegans DNA will be added to modified histones and to unmodified histones and allowed to create nucleosomes. The wrapped DNA will be sequenced, allowing us to compare the modified and unmodified nucleosome DNA preference. The difference in preference will enhance our ability to know how to move nucleosomes, thus aiding in gene therapy.

Spencer Foutz, Brigham Young University Biology CSF AB42 levels are a biomarker for Alzheimer’s Disease. The APOE e4 allele associates with CSF AB42. Little is known about SNPs in the region independent of apoe e2/e3/e4 isoforms. By adjusting for the effect of these isoforms, statistical analysis uncovered new SNPS associated with CSF AB42. Information was used from 1338 individuals from four datasets, specifically: The WU-ADRC, ADNI, University of Washington, and UPENN. Samples included individuals with and without AD. The 169 SNPs used were extracted from the APOE region and surrounding 50 kb using 1000 Genome Software. Linear regression analysis was performed, adjusting for specific covariates. Adjustments were made for the APOE e2 and e4 alleles before repeating the analysis. Significant SNPs were tested in e3 homozygous individuals. Each series was separately analyzed and combined in a meta-analysis for confirmation. P-values, sample sizes, and effect sizes were used in the meta-analysis. Results from these analyses allowed us to conclude rs769449 is associated with lower levels of CSF AB42 and acts independent of the APOE e4 allele.

Jason Bartholomew, Brigham Young University Plant and Wildlife Sciences After disturbances in plant communities (i.e. wildfire), there is a natural succession of plants in which plants colonize the empty area and are gradually replaced by more competitive species. In subalpine forests, the principle colonizers after wildfire are quaking aspen (Populous tremuloides) which are later replaced by subalpine fir (Abies lasiocarpa). It has been shown that aspen facilitate, or enable, the establishment of subalpine fir at their base. This study examines the aspen-subalpine fir interaction in order to better understand the dynamics of the shift from aspen to fir dominance. It is hypothesized that the fir in a facilitated pair eventually exerts a competitive influence on the aspen resulting in a decrease in aspen fitness. The growth rates of the two species were examined in different stand types (aspen, mixed and subalpine fir), as independent trees or in facilitated pairs, and in three separate size classes. Samples were collected by taking a core sample or cross-section from trees within the categories listed above. The age and annual growth rings were measured with a measuring stage. The annual growth rings were used to calculate basal area increase (BAI) which was used to determine growth rates. The results suggest the growth rate of aspen in facilitated pairs decreases as firs mature thereby decreasing fitness within the aspen population due to competitive influences from facilitated firs. This may explain the mechanism for the successional shift that can significantly impact indigenous animal populations and local fire cycles.

Rachel Schneider, Brigham Young University Neuroscience The ability to create distinct memories for very similar stimuli and events is called pattern separation. Pattern separation is thought to be dependent on neurogenesis (the birth of new neurons) in the dentate gyrus, a subregion of the hippocampus. Neurogenesis is reduced in depression, as is overall memory performance. It has been proposed that depression negatively impacts pattern separation abilities, however a link between depression and performance in pattern separation memory tasks has yet to be identified. Accordingly, we designed a study to investigate the relationship between pattern separation performance and level of depression. Eighty-two participants completed a pattern separation memory test and a set of questionnaires to gauge their level of depression. During the task, participants were presented with 600 images one at a time on a computer screen in a continuous recognition paradigm. Participants were asked to determine whether each image was new, old, or similar. Images seen for the first time during the task qualified as “new”, images that were repeated following a variable delay qualified as “old”, and images that were similar to previously presented stimuli, but not exactly the same, qualified as “similar”. A pattern separation score was calculated based on the proportion of correctly identified similar stimuli. We found a negative correlation between depression scores and pattern separation scores (r(82) = – 0.301, p < 0.01). This relationship held constant even when we controlled for other factors known to affect neurogenesis, such as exercise and anxiety levels. These results provide support for the theory that depression is negatively related to pattern separation performance, possibly due to a decrease in neurogenesis in the hippocampus.

Kevin Boehme, Brigham Young University Biology Late Onset Alzheimer disease (LOAD) is caused by a complex combination of genetic and environmental factors. While multiple loci have been found associated with an increased risk of LOAD much of the heritability of the disease has yet to be accounted for. The prevailing thought now is that of rare variants playing an important role in LOAD. In this study we will use linkage analysis to identify novel regions of the genome that may harbor rare disease causing variants. Data for these analysis comes from 748 people (503 with LOAD) from the Cache County study on Memory and Aging. This unique population based sample provides great power for linkage as relatedness differs from siblings to distant relatives and complete pedigree information is available for all of the individuals. We will use LD-pruned SNP data from the Illumina Omniexpress BeadChip and pedigree data from the Cache County samples to perform linkage analysis. Quality control and LD-pruning will be con- ducted in PLINK while the Linkage analyses will be conducted using the MERLIN software. Our findings will be reported in the final poster presentation.

Spencer Bell, Brigham Young University Physiology and Developmental Biology The hippocampus functions as the memory formation center of the brain. As memories are formed, brain cells in this area undergo changes by which connections between them are either strengthened or weakened, processes known as long-term potentiation (LTP) and long-term depression (LTD), respectively. Receptors located on these cells modulate these processes as they are activated by chemical signals known as neurotransmitters. We sought to examine the effects of a receptor known as G protein-coupled receptor 55 (GPR55) on LTP and LTD in the rodent hippocampus by applying agonists of the receptor, or chemicals that artificially activate it, to brain slices preserved in artificial cerebrospinal fluid. O-1602 is a purported synthetic agonist of GPR55. When we applied O-1602 to rat hippocampal slices during electrical induction of LTP, the magnitude of LTP was decreased when compared to controls. When LTD was induced in the rat hippocampus in the presence of O-1602, no significant difference was observed when compared to controls. Further experimentation involved the application of lysophosphatidylinositol (LPI), a naturally occurring GPR55 agonist, to genetically-engineered knock-out mice which lacked expression of GPR55. LPI is generally considered a more reliable agonist of GPR55, but caused enhanced LTP in wild-type mice which expressed GPR55 when compared with knock-out mice. This inconsistency and other inconsistencies in our data while using O-1602, while perhaps due to other physiological differences between rats and mice, may suggest the possibility that O-1602 activates a receptor other than GPR55. Our further research will seek to investigate this possibility.

Kayla Bevard, Brigham Young University Microbiology and Molecular Biology Metabolic diseases, such as obesity and diabetes, are endemic in American society. Mutations in PAS kinase, a recently discovered sensory protein kinase, have been shown to cause Maturity Onset Diabetes of the Young (MODY) in humans (Semplici et al., 2011). In addition, PAS kinase deficient mice, when placed on a high fat diet, display phenotypes related to diabetes including resistance to weight gain, insulin insensitivity and triglyceride accumulation (Hao et al., 2007). PAS kinase consists of a sensory PAS domain that binds to and inhibits the kinase domain (Amezcua et al., 2002). Our model for PAS kinase activation involves the generation of a small molecule ligand that binds to the PAS domain and relieves this inhibition. We are currently engaged in several yeast genetic screens which will identify regions in the full length PAS kinase protein that are essential for either PAS kinase activation or for binding of its substrates. The first screen is based on the ability of PAS kinase, when overexpressed, to rescue a temperature-sensitive mutation in Tor2, the tor2(ts). We have isolated both point mutations and truncations in PAS kinase which alleviate the tor2(ts). These mutations solidify our model for PAS domain inhibition and will identify novel regions involved in PAS kinase regulation. Our second screen uses the yeast 2-hybrid to select for both point mutations and truncations that increase the affinity of PAS kinase for its substrate, Pbp1. These mutations will help identify key regions of PAS kinase utilized in substrate recognition. Finally, we will compare the regions affected by our mutations with the regions of PAS kinase that we have found to be well-conserved throughout evolution. Analysis of these specific genetic regions will help elucidate the molecular mechanisms involved in the regulation and function of PAS kinase, a key player in the development of metabolic disease.

Rachel Nettles, Brigham Young University Plant and Wildlife Sciences Background/Questions/Methods

Caitlin Munger, Brigham Young University Biology Alzheimer’s is a fatal, non-treatable neurodegenerative disease and the most common cause of dementia. While no one gene has been found to determine the development of Alzheimer’s, past studies have established a strong hereditary influence on Alzheimer’s. So far, only 5 genes have been found which replicably contribute to the genetic risk of developing Alzheimer’s. However, the gene for Chlolesteryl Ester Transfer Protein (CETP) has been identified as a possible new contributor to the genetic risk factor. In order to test this association we obtained data on over 4000 subjects studied in the Cache County Study on Memory, Health and Aging over a 15-year period. This data included DNA samples, cognitive decline rates and incidence of dementia–particularly Alzheimer’s Disease. DNA samples were SNP genotyped using quantitative PCR. The SNP genotypes and corresponding phenotypes for each subject were then analyzed for association usingmixed linear models and for survival, or the amount of time until the disease appeared, using Cox proportional hazard models. We found a correlation between the V405I SNP and a decreased rate of cognitive decline. We found that for each additional G the rate of decline decreased by 0.6 points per year on the MMSE test. The identification of CETP as a player in the genetic risk for Alzheimer’s and dementia will provide much needed information on the genetic factors involved in dementia and allow for possible future therapeutic targets.

Joseph Moulton, Brigham Young University Physiology and Developmental Biology With the advent of recent mutations in the influenza A viral genome, drugs that previously blocked the proton flux responsible for disassembly of the viral envelope and exposure of viral RNA to the transcriptional machinery of the host cell have become ineffective. Our study of the M2 hydrogen ion channel responsible for this flux has led to a vastly-increased under- standing of the mechanisms behind the conductance activity and potential blockage of these transmembrane tetramers. By embedding M2 proton channel subunits of the S31N mutant strain into liposomal bilayers and suspending these bilayers in the buffers and ionic gradients characteristic of the intracellular environment, we have been able to simulate and observe nor- mal functioning of the influenza A virus. Using these liposomal bilayers, we have developed a series of experimental protocols to test a variety of amantadine- and rimantadine-related drugs for successful blockage of M2 S31N proton conductance. Our research presentation will be centered around the mechanisms of this channel and the favorable results that we have obtained from many of these drugs.

Anindita Ravindran, University of Utah Exercise and Sport Science Obesity predisposes individuals with Type II Diabetes to cardiovascular complications such as impaired blood vessel function. Due to the elevation of free fatty acids (FFAs) in obese individuals, ceramide, a lipid metabolite, accumulates and might contribute to the inability of a blood vessel to constrict or relax appropriately. Vessel dysfunction is partly caused by the inability of the endothelium, the innermost protective lining of blood vessels, to synthesize and release nitric oxide (NO). Our data indicate that ceramide impairs endothelial NO synthase (eNOS), the enzyme that synthesizes NO. In order to study mechanisms by which ceramide might impair eNOS, it is important to measure cellular ceramide production in response to pharmacological and genetic manipulations. Previously we used P-32 radioactive assays to measure ceramide accumulation. However, the use of radioactivity is expensive, potentially hazardous, and waste disposal is an environmental concern. Therefore, I sought to import a less harmful, more cost effective, yet accurate technique of measuring ceramide production by immunofluorescence (IF). IF allows ceramide to be tagged with a primary antibody which can be detected by a secondary antibody conjugated with a fluorescent dye. I have observed that 250, 500, and 750 uM palmitate (pal) incubation for 3 h increases (p<0.05) endothelial cell ceramide accrual in a dose-dependent manner. Further, a FFA-independent method to alter ceramide accrual i.e., 3 h incubation of cells with N-oleoylethanolamine, also elevates (p<0.05) ceramide production. Importantly, I have shown that 500 uM palmitate-induced ceramide accrual can be prevented (p<0.05) by two structurally dissimilar inhibitors (10 uM myriocin, 1mM L-cycloserine) of the rate-limiting enzyme responsible for ceramide biosynthesis i.e., serine palmitoyl transferase (SPT). None of these inhibitors impairs cell viability. These data indicate that IF is an accurate and reproducible method whereby ceramide accrual can be quantified in endothelial cell systems.

Makda Gebre, Utah State University Biology The first pandemic of the 21st century was the influenza A (H1N1pdm09) virus that originated in Mexico and killed 12,000 people within the United States. It now circulates as a seasonal influenza virus causing mild symptoms in most but still killing some susceptible individuals. Influenza A (H1N1pdm09) virus leads to acute respiratory distress syndrome (ARDS) in high risk patients and has a mortality rate of 40-50% in those patients. ARDS patients usually die before any intervention since there is no clinical evidence that can be used to detect it. To detect ARDS early in disease progression, we need to search and develop biomarkers that can be tested in the patient’s blood or fluids. The three biomarkers tested as potential early disease indicators in this project included: C-reactive protein (CRP), serum amyloid A (SAA) and Transferrin. To test these biomarkers, mice were infected with the influenza A H1N1pdm09 virus and serum was collected from the mice at different time points. Serum collected was tested for amounts of CRP, SAA and Transferrin using an enzyme-linked immunosorbent assay (ELISA) and spectrophotometer. Control mice were mock infected and also tested for the biomarkers for comparison.

Thomas Anderson, Utah State University Biology Pradimicin, a small molecule produced by the soil bacterium Actinomadura hibisica, is a promising candidate as a combined antifungal/antiviral therapeutic. It is active against a broad-spectrum of opportunistic, pathogenic fungi, interferes with the replication of influenza virus, and inhibits the reproduction of HIV-1. Toxicity and solubility problems have hindered past efforts to develop pradimicin as a therapeutic. Our research focuses on elucidating the bio-synthetic pathway of pradimicin in order to design and chemoenzymatically create pradimicin structural analogs with improved solubility and activity, and less toxicity. Several enzymes in pradimicin biosynthesis have been identified. We intend to characterize one of the key enzymes, PdmS, a putative glycosyltransferase, and to manipulate its gene to create novel, more efficacious pradimicin analogs. This project is funded for three years by the NIH NIAID (3 years). Methods: A gene knockout experiment was used to determine the role of PdmS in pradimicin production. Bio-synthetic precursors of pradimicin were subjected to bio-transformations in E. coli with recombinant genes for PdmS and another glycosyltransferase, OleD, to generate analogs with new sugar attachments. Analogs of pradimicin will be screened for bio-activity using standard microbroth dilution assay techniques. Confirmed results: The enzyme PdmS was identified and characterized as a glycosyltransferase. Expected results: newly created analogs of pradimicin exhibited minimal inhibitory concentrations of 10μg/mL against Candida albicans. Conclusion: Knockout of pdmS yielded the pradimicin aglycon, which confirmed the function of this glycosyltransferase and provides a start molecule for further structural modification to yield new analogs for bioactivity studies.