2013 Abstracts

Melissa Lewis, Dixie State University English Both George Eliot and Thomas Hardy have been called determinists partly as a result of their novels The Return of the Native and The Mill on the Floss. The role of fate or destiny largely guides the plot of these works, regardless of the decisions and merit of the characters. A comparison of the style of determinism between the two authors reveals that George Eliot emphasizes a moral force while Thomas Hardy focuses on a force associated with nature. Eliot’s determinism suggests that the world is determined but that humans still remained morally responsible for their actions, as Maggie is responsible for not feeding Tom’s rabbits or for her lack of decision as she drifts down the river with Stephen Guest in The Mill on the Floss. Hardy tends to be more of a naturalist. The naturalist is a product of post-Darwinian theory that humanity is controlled entirely by nature. For example, the fate of Eustacia Vye in The Return of the Native seems to be controlled by the hand of Egdon Heath. Both novels depict extraordinary characters in a world bent to squash them. These novels reveal that the individual exists as a solitary entity, and the relationship that used to exist between God and individual is now between the individual and society. Both Eliot and Hardy create novels where the world seems determined and cruel and characters that don’t adhere or fit in this mold are eliminated or reduced.

Hanna Higginson, University of Utah Anthropology The study, “Sentimentality Preserves Our Past” focuses on two branches of nostalgia: sentimentality and historical utility and their relationship to cultural relics. Our approach assumes we can recognize similarities and differences between cultural communities by describing the extent to which individuals are sentimental or utilitarian with regard to their possessions. We are currently collecting data from the northern Utah community and Utah Tongan community. We set up a preliminary exercise asking participants to free list the items they have a difficult time throwing away for sentimental reasons. So far we have collected 40 questionnaires and 13 interviews that focus on the five most referenced items. Our target is to conduct about 45 questionnaires and 45 interviews for each community. For the Utahan population we have enough data to identify an S:U RANK – the ratio between a participant’s measure of sentimentality to the measure of utility. For the 25 questions where a respondent’s answer could be classified as appealing to sentimentality (s) or historic utility (h), we summed the totals then divided it by the sum of the answers appealing to utility (u). All totals above 1 indicate that the respondent prefers protecting items for nostalgic purposes; all answers below 1 indicate the respondent prefers items of utility. The S:U RANK gives a general view into a particular population. From this we can discuss the effect of variables such as age, marital status, children, age of children, and years in Utah on an individual’s preference. Currently, the pattern from the S:U RANK suggests that marital status and children are having the greatest effect on the ratio, but this will become clearer as the interviews progress. The research began as an effort to understand the presence of nostalgia as applied to five common relics among a particular diaspora, in light of the incoming data, this question is developing into an attempt to understand nostalgia in a cultural and universal sense as well.

Betty Stoneman, Utah Valley University Philosophy The “American Dream” and the “Working Class Promise” are ubiquitous ideologies in American culture. For this paper, I will argue these ideologies are social constructs which perpetuate and reinforce discrimination, the social hierarchy and the domination of the American working class. First, I will define the key concepts of this paper: ideology, stereotyping, domination, discrimination, the ideologies of the American Dream and the Working Class Promise. Secondly, I will argue these ideologies, as defined by communications professor Kristen Lucas, lead to discrimination and domination of the working class. I will argue the Working Class Promise is an example of philosopher Frederick Nietzsche’s slave morality, where a lower status group attaches positive values to themselves which only serves to perpetuate discrimination against them. Further, I will argue domination arises from such discrimination by examining a study by psychologists Shannon K. McCoy and Brenda Major regarding positive stereotyping, self-stereotyping, discrimination and domination. Next, I will argue, using sociologist Pierre Bourdieu’s concept of habitus, both the Working Class Promise and the American Dream are structured and structuring social concepts which reinforce discrimination and domination through exclusion. In line with Bourdieu, I will use social philosopher Louis Althusser’s arguments to show how the Working Class Promise and the American Dream reproduce the roles of the social hierarchy and domination. I will support this demonstration with evidence from McCoy and Major’s study showing how various forms of discrimination are reproduced in society when individuals believe in the American Dream. Having accepted the social insights of these scholars, I would propose, based on the arguments of philosopher Immanuel Kant regarding the intrinsic worth of rational beings and contrary to elitist or socialist views, the solution is for Americans to reject these ideologies.

Echo Smith, University of Utah English and Classics In his novel Querelle Jean Genet depicts homosexual relationships among men, primarily in the navy, as narrated by the character of Lieutenant Seblon. The main object of Seblon’s affections and infatuations is Querelle, after whom the work is titled, the reigning protagonist. As the novel quite overtly depicts the occurrence of sexual acts between men, it is easily read as homosexual literature. However, what I argue is that homosexuals, within the novel, are identified more by verbal expressions than acts of the body. Throughout the text, Genet frequently draws attention to the mouth and the ways in which it expresses, imbibes, or expels. Using Georges Bataille’s theories of the mouth, which also focus on a type of oral release, I opine that one can see the relationship between this orifice and the way in which we, as humans, take in or eject things from our bodies. This notion coupled with Genet’s employment of the mouth as a means of homoerotic expression provides for a queering of the mouth to occur. Therefore, I conclude that the mouth, in the text of Querelle, becomes the orifice through which homosexuality is released from the body, primarily through verbal expression, and becomes the more accurate indicator of the homosexual within the text.

April Jackson, Dixie State University English With the publication of Kate Chopin’s complete works in 1969, proponents fighting for a new paradigm regarding women’s choices in regard to sexuality, marriage, and motherhood found much to consider in her work. Often, critics will analyze Chopin’s works with a limited feminist lens, focusing on themes of marriage, sexuality and sexual independence, and the creation of self-identity through marriage and sexual awakenings. However, Chopin’s works do not focus on women and the world they face to the exclusion of men; in writing about women and their lives, she also draws attention to the effects the attitudes of men have on women. Focusing on Chopin’s male characters, who often serve as satellite figures, this paper will analyze the way men in her texts can create an atmosphere in which women can, without fear of societal rejection, shape their own lives. In her larger body of works, two men exemplify this ideal man: Gouvernail, who appears in “Athenais” and “A Respectable Woman”; and Offdean, who appears in “A No-Account Creole.” These two men represent a model of masculinity that is most conducive for women to fully assert and create their sexual and social independence and self-identity. With the study and analysis of these satellite male characters, rather than limiting the analysis to just the women’s experiences, deeper and newer readings of Chopin’s work will surface and allow for additional themes to come forward in the broader area of Chopin studies.

Catherine Howell-Dinger, University of Utah English While feminist projects have often acknowledged that discourse creates specific material conditions and lived realities, it has rarely been asked how these conditions might influence discourse itself. This paper investigates the politics of textual production and argues that some texts have thought of the material conditions of the narratives which they disclose as inseparable from, and present in, the text itself; however, other texts show a high level of anxiety about their relationship to materiality, and ultimately attempt to suppress this relationship as a means of ensuring a particular privileged position. For example, Toni Morrison’s Beloved has been read by some critics as an example of Kristeva’s semiotic. Pursing this reading further, I argue that a more active conception of the body is present in Morrison’s work than was previously observed by these critics. In Morrison’s work, the body is not simply something that is written; rather, it is something that actively shapes and inflects the text vis-a-vis its own disruption of the text. On the other hand, although the body seems to figure prominently in the work of some authors, such as James Tiptree’s “The Girl Who was Plugged In,” its relationship to textual production is eclipsed in order to maintain political power and hegemonic masculinity. The authorial voice in Tiptree’s work ultimately attempts to cover over its own connection to the feminine body of its composer, Alice Sheldon, in order to give its own feminist message authority.

Carlos Junior Guadarrama, Utah State University English My poster explores the history of the Migrant Head Start program at the former Indian Intermountain School location in Brigham City, Utah. No one to my knowledge has gathered a history of this program, which operated from the mid-1980s until the early 2000s. I intend to explore this Migrant Head Start’s foundations as an informal school for the children of Latino migrants, as well as how it grew and developed over the almost two decades that it existed, before it became the Centro de la Familia de Utah. I plan to interview a former principal, several teachers, as well as former students. I argue that this Head Start played an extremely important educational and social role in the lives of many inhabitants of Brigham City.

Jeffrey Green, University of Utah Linguistics Although learning a second language can be difficult, especially for adults, many people believe that once an individual has acquired a second language, it will be easier to learn a third. Individuals may utilize strategies learned from studying a second language (L2) to their study of a third, but this does not necessarily mean that learning a third language (L3) will be easier. As individuals study an L3, their knowledge of both their native language (L1) and their L2 will compete with their developing knowledge of the L3. Previous research has shown that during early stages of L3 acquisition, both L1 and L2 phonology (that is, the underlying sound system of a language) influence the phonology of their L3. This is evident, for example, in learners’ production of some word-initial consonants, such as d, p, and k. Languages differ in the timing between the articulation of the consonant and the beginning of vibration of the vocal folds (voicing) associated with a following vowel sound. This timing is known as Voice Onset Time (VOT). Studies suggest a stronger influence from L2 than from L1 in L3 VOT production. However, learners in these studies had some (if limited) knowledge of the L3, and the influence of this knowledge is unclear. In addition, previous studies have addressed L3 production, but not L3 perception. Research has yet to investigate (i) the influence of L1 and L2 phonology in the very initial stages of L3 acquisition (when the language is totally unfamiliar), and (ii) the influence of L1 and L2 phonology on the perception of VOT boundaries. This study investigates (i) and (ii) by presenting English and Spanish bilinguals with stimuli representing a range of VOT in their L1 and L2, as well as from a third, unfamiliar language in a series of tasks designed to elicit evidence of VOT boundaries in each language. The results of these tasks will be examined to determine whether learners rely more on their L1 or their L2 for processing a third, unfamiliar language. Preliminary results and analyses will be available by February 2013. This research will give important insights into the process of acquisition of a third language.

Ryan Krage, Utah Valley University English and Literature Within Freshman Composition Studies, little research has been produced on writing conferences between students and teachers since Laurel Johnson Black’s Between Talk and Teaching: Reconsidering the Writing Conference in 1998. Most published research has not included any systematic information about the nature of the writing conference, a consultation between the student and teacher designed to discuss the student’s paper in order to make changes. Student feedback and involvement in this study is of crucial importance because there is no existing data whether teacher-student conferences are working from a student perspective. The major research question is “What is the relationship between types of teacher-student conference interaction and subsequent revision of students’ papers?” The purpose of the research is to discover what the writing conference between teacher and student accomplishes in terms of better writing outcomes, what the expectations of the student are, and what and how the power dynamics between student and teacher affect the quality and quantity of revision from both student and instructor perspectives. We will examine the connection of students’ perceptions about the writing conference to their final scores and other data. During the spring 2012 semester, we drafted three surveys designed to capture students’ perspectives and expectations regarding the conferences (a pre-conference, post conference, and post final grade). The methodology includes transcribing voice recordings of conferences, collecting student surveys before and after conferences, and collecting students’ rough and final drafts for two papers during the semester. This raw data will be coded into usable data, which will then be analyzed to identify behaviors that facilitate or impede the conference and determine its overall effectiveness. We hypothesize that a dialogic conference will occur when the power dynamic between student and teacher is more equitable, resulting in more student-ownership over the revision process. The extent of student ownership within the revision process will be determined by both the quantity and quality of revision. These findings will, in turn, enable teachers to take a closer look at the nature of their own writing conferences in order to develop better consultations with their students.

Michael McEntire, Brigham Young University Biology Female mate choice (intersexual selection) and male dominance interactions (intrasexual selection) can each play important roles in sexual selection. These two mechanisms tend to be discussed in isolation. The goal of this study is to explore the interaction between these two forms of sexual selection. To test this idea, we focused on the livebearing tropical fish system Poecilia gillii. We grouped males into similarly sized pairs and observed them for a week to determine which male was dominant. These pairs were then presented to females in mate choice trials to ascertain female preference. We also photo- graphed the males to determine coloration. We found that females were unable to detect dominant males without viewing the physical contest and that carotenoid coloration bore no effect on female preference. Females tended to choose the male to their left, suggesting the preferential use of their right eyes in making decisions on mate choice.

Whitney Hoopes, Brigham Young University Microbiology and Molecular Biology HspB2 is one of eleven known small Heat Shock Proteins (sHSP) that is expressed in human heart and skeletal muscle. In response to cellular stress, heat shock proteins play a vital role to help misfolded proteins and proteins susceptible to denaturation maintain their structure. Two members of the sHSP family, CryAB and HspB2, are both required for normal heart function and cardiac muscle integrity. CryAB-deficient mice have defects in cardiac muscle structure whereas HspB2-deficient mice display energy deficits (Rajasekaran et al. 2007). The contrasting phenotypes of CryAB and HspB2 suggest differential roles for these molecular chaperones in the heart. HspB2 has been found to localize with the mitochondria in several different cell lines and overexpression of this sHSP has been shown to support survival of cells against heat stress (Nakagawa, 2001). To understand the role and mechanism of HspB2 in cardiac muscle energy regulation, we have used a yeast two-hybrid (Y2H) system to uncover the novel protein binding partners specific to HspB2. From screening a human heart cDNA library, HspB2 interacted with approximately 10,000 out of 20 million plasmids. We have sequenced more than 1000 of these putative interactors and have identified over 100 unique proteins. Over 40% of these protein partners are involved in mitochondrial energy production and another 25% in cardiac muscle structure maintenance. In addition, we have identified an interaction between HspB2 and the related sHSP CryAB. We then compared this data with mitochondrial HspB2 binding partners identified by mass spectroscopy (MS) through a large-scale bioinformatics analysis and constructed a protein-protein network. Y2H dependency tests were conducted to verify interactions identified by both Y2H and MS. Following yeast verification, a subset of the interactions were confirmed in C9H2 cardiac cells through coimmunopurification. Our research describes the first protein-protein interaction network for any sHSP, supports a role for HspB2 in mitochondrial energy production and suggests a link between mitochondrial energy production/redox stasis and stressed cardiac muscle maintenance.

Christian Boekweg, Brigham Young University Plant and Wildlife Sciences It has been shown that the population densities of deer and elk in the Rocky Mountains are at an unprecedented high. The heavy browsing of said species (ungulates) on regenerating aspen suckers can be devastating, leading to homogenously aged aspen stands that now have reduced resilience to drought, fire or logging disturbance, and an increased susceptibility to pathogens. Lastly, the extensive, highly selective herbivory of the aspen suckers may cause a shift in forest composition away from the aspen tree to an increase in other, less palatable species. We selected 186 sites across the 3 national forests of Central and Southern Utah, and characterized stand composition using the point quarter method. Stands were defined by successional stages from early to late; aspen, mixed or conifer. We then used pellet counts to estimate animal density and evaluated the effect of animal density and stand type on the regeneration success of aspen suckers. The key result of our study is that high ungulate density is highly correlated with reduced sucker heights (p<0.001). This indicates that high ungulate density impedes aspen regeneration by preventing aspen from recruiting into the overstory. Our study suggests that closer monitoring of the long term effects of herbivory on aspen development and regeneration is necessary to ensure vigorous aspen forests.

Jesse Cobell, Brigham Young University Biology Alzheimer’s disease (AD) is the sixth major cause of death in the U.S. However, at present, no diagnostically useful serum markers for AD have been identified. Hence, we used a novel serum proteomic approach to interrogate the low molecular weight proteome for serum biomarkers. This allowed for survey of around 5000 low MW species. To reduce ion suppression, an acetonitrile precipitation step was used to remove high abundance serum proteins. Protein-depleted sera from 58 cases and 55 controls were analyzed by cLC-ESI-QTOFMS/MS using reverse phase chromatography. Data were reviewed using Applied Biosystem’s Analyst-QS software to compile spectra. Differentially expressed peptides (cases vs. controls) were analyzed statistically using the Student’s t-test. This led to discovery of 36 candidate biomarkers. Additionally, we compared AD subjects with more severe disease (Clinical Dementia Rating (CDR) =3) with non-demented individuals (CDR=0) and found 23 biomarkers. Furthermore, on comparison of mild and moderate stage AD individuals (CDR = 0.5, 1, 2) with those with severe disease (CDR = 3), we found 24 biomarkers. Some of these biomarkers appeared more prominent in one gender. We then fragmented several of these biomarkers on an LTQ-Orbitrap XL hybrid mass spectrometer and cLC-ESI-QTOF-MS/ MS system using collision-induced dissociation to determine amino acid sequence analysis. We have identified 5 biomarkers and are in the process of identifying the remaining biomarker species. This serum proteomics approach found statistically different peptide abundances in subjects with AD. Additional biostatistical evaluations are underway to determine sensitivity and specificity of individual biomarkers and their combinations. Future studies will assess biomarkers according to disease stage and validate current biomarkers in blinded comparisons of other AD sera. This serum proteomics approach appears promising in locating and identifying clinically useful serum biomarkers of AD.

David Marriott, Brigham Young University Physiology and Developmental Biology Acute stress has been shown to decrease Long-Term Potentiation (LTP) in the CA1 region of the mouse hippocampus. Additionally, stressed animals show signs of anxiety and suffer decreases in spatial memory tasks such as object recognition and maze navigation. Conversely, exercise has been shown to increase spatial memory task performance in mice, attenuate anxiety-like behaviors and enhance neurogenesis and LTP in the dentate gyrus. While the effects of stress and exercise have been examined independently, there is currently a lack of experimental evidence that connects how stress and exercise, when experienced by the same animal, might modulate LTP in the CA1 region of the hippocampus. In our ongoing study, mice have been separated into a control group, a stress group (restraint and tail-shock), and an exercise + stress group where mice have voluntary access to a running wheel (for 30 days) before undergoing the stress protocol. We hypothesize that exercised animals will experience a protective effect against the reductions in CA1 LTP. In the stress only group, preliminary data shows a modest stress effect on LTP, yet we are learning that factors such as controllability of the stressor or the ability to develop coping mechanisms might potentially attenuate

Tara Hammond, Brigham Young University Microbiology and Molecular Biology Genetic diseases, including Alzheimer’s, cystic fibrosis, and many cancers, can be detrimental to individuals and their families. Gene therapy can possibly cure these diseases by inserting a correct copy of the gene into the chromosome, upregulating good genes, or downregulating the harmful gene. When DNA is packaged into a cell, it wraps around histones-an octamer made up of two tetramers, each containing four different subunits to create nucleosomes. Where the nucleosome sits on the DNA sequence determines whether or not a gene can be transcribed. In heterochromatin, nucleosomes are denser and DNA is tightly packed, thus causing genes to not be transcribed. Euchromatin contains looser packed nucleosomes and therefore has higher transcription levels. This project seeks to determine if modified nucleosomes have DNA sequence preferences. We are working with histone H3 to tri-methylate lysine 4, which has been shown to correlate with euchromatin. The modified histone will be used to create octamers. C. elegans DNA will be added to modified histones and to unmodified histones and allowed to create nucleosomes. The wrapped DNA will be sequenced, allowing us to compare the modified and unmodified nucleosome DNA preference. The difference in preference will enhance our ability to know how to move nucleosomes, thus aiding in gene therapy.

Spencer Foutz, Brigham Young University Biology CSF AB42 levels are a biomarker for Alzheimer’s Disease. The APOE e4 allele associates with CSF AB42. Little is known about SNPs in the region independent of apoe e2/e3/e4 isoforms. By adjusting for the effect of these isoforms, statistical analysis uncovered new SNPS associated with CSF AB42. Information was used from 1338 individuals from four datasets, specifically: The WU-ADRC, ADNI, University of Washington, and UPENN. Samples included individuals with and without AD. The 169 SNPs used were extracted from the APOE region and surrounding 50 kb using 1000 Genome Software. Linear regression analysis was performed, adjusting for specific covariates. Adjustments were made for the APOE e2 and e4 alleles before repeating the analysis. Significant SNPs were tested in e3 homozygous individuals. Each series was separately analyzed and combined in a meta-analysis for confirmation. P-values, sample sizes, and effect sizes were used in the meta-analysis. Results from these analyses allowed us to conclude rs769449 is associated with lower levels of CSF AB42 and acts independent of the APOE e4 allele.

Jason Bartholomew, Brigham Young University Plant and Wildlife Sciences After disturbances in plant communities (i.e. wildfire), there is a natural succession of plants in which plants colonize the empty area and are gradually replaced by more competitive species. In subalpine forests, the principle colonizers after wildfire are quaking aspen (Populous tremuloides) which are later replaced by subalpine fir (Abies lasiocarpa). It has been shown that aspen facilitate, or enable, the establishment of subalpine fir at their base. This study examines the aspen-subalpine fir interaction in order to better understand the dynamics of the shift from aspen to fir dominance. It is hypothesized that the fir in a facilitated pair eventually exerts a competitive influence on the aspen resulting in a decrease in aspen fitness. The growth rates of the two species were examined in different stand types (aspen, mixed and subalpine fir), as independent trees or in facilitated pairs, and in three separate size classes. Samples were collected by taking a core sample or cross-section from trees within the categories listed above. The age and annual growth rings were measured with a measuring stage. The annual growth rings were used to calculate basal area increase (BAI) which was used to determine growth rates. The results suggest the growth rate of aspen in facilitated pairs decreases as firs mature thereby decreasing fitness within the aspen population due to competitive influences from facilitated firs. This may explain the mechanism for the successional shift that can significantly impact indigenous animal populations and local fire cycles.

Kevin Boehme, Brigham Young University Biology Late Onset Alzheimer disease (LOAD) is caused by a complex combination of genetic and environmental factors. While multiple loci have been found associated with an increased risk of LOAD much of the heritability of the disease has yet to be accounted for. The prevailing thought now is that of rare variants playing an important role in LOAD. In this study we will use linkage analysis to identify novel regions of the genome that may harbor rare disease causing variants. Data for these analysis comes from 748 people (503 with LOAD) from the Cache County study on Memory and Aging. This unique population based sample provides great power for linkage as relatedness differs from siblings to distant relatives and complete pedigree information is available for all of the individuals. We will use LD-pruned SNP data from the Illumina Omniexpress BeadChip and pedigree data from the Cache County samples to perform linkage analysis. Quality control and LD-pruning will be con- ducted in PLINK while the Linkage analyses will be conducted using the MERLIN software. Our findings will be reported in the final poster presentation.

Bryce Anderson, Brigham Young University Microbiology and Molecular Biology Antigen presenting cells digest and display peptides from foreign and infected cells on the major histocompatibility complex (MHC) that are recognized by T-cells through their T cell receptor (TCR). The affinity of TCR:peptide-MHC interactions has been shown to be low however, and in order to effectively use a soluble TCR for therapeutics we need to engineer TCRs with increased affinity. To do this, we have designed a single chain TCR (ValphaVbeta) called LLO118 that is specific for a naturally occurring Listeria monocytogenes epitope. Using yeast display, stable mutants that expressed the LLO118 scTCR at higher levels than the wild type on the surface of yeast were isolated and sequenced. In order to improve affinity of LLO118 we are mutating amino acid residues in the complementarity determining regions, sites important for the TCR to bind with the peptide-MHC. We are generating unique libraries of yeast cells with TCRs that have potential affinity mutations and using fluorescently labeled peptide-MHC tetramers to select cells that have TCRs with higher affinity. By repeating this process with the cells that have higher affinity we are working to get a TCR that binds with much higher affinity than the wild type TCR. These high affinity TCRs are promising for further research in connecting them to a cytokine, greatly reducing systemic damage and other complications caused by administration of this cytokine throughout the body. Thus, our goal is to design a high affinity TCR fused to a cytokine that can be tested for therapeutic use in targeting specific cells in the immune response and improving T cell memory.

Spencer Bell, Brigham Young University Physiology and Developmental Biology The hippocampus functions as the memory formation center of the brain. As memories are formed, brain cells in this area undergo changes by which connections between them are either strengthened or weakened, processes known as long-term potentiation (LTP) and long-term depression (LTD), respectively. Receptors located on these cells modulate these processes as they are activated by chemical signals known as neurotransmitters. We sought to examine the effects of a receptor known as G protein-coupled receptor 55 (GPR55) on LTP and LTD in the rodent hippocampus by applying agonists of the receptor, or chemicals that artificially activate it, to brain slices preserved in artificial cerebrospinal fluid. O-1602 is a purported synthetic agonist of GPR55. When we applied O-1602 to rat hippocampal slices during electrical induction of LTP, the magnitude of LTP was decreased when compared to controls. When LTD was induced in the rat hippocampus in the presence of O-1602, no significant difference was observed when compared to controls. Further experimentation involved the application of lysophosphatidylinositol (LPI), a naturally occurring GPR55 agonist, to genetically-engineered knock-out mice which lacked expression of GPR55. LPI is generally considered a more reliable agonist of GPR55, but caused enhanced LTP in wild-type mice which expressed GPR55 when compared with knock-out mice. This inconsistency and other inconsistencies in our data while using O-1602, while perhaps due to other physiological differences between rats and mice, may suggest the possibility that O-1602 activates a receptor other than GPR55. Our further research will seek to investigate this possibility.

Brad Ackerson, Brigham Young University Neuroscience The highjacking by alcohol and drugs of abuse of the mesocortico-limbic system in the brain is responsible for addiction, specifically the ventral tegmental area (VTA) and its projecting dopaminergic neurons to the nucleus accumbens (NAc). Over the course of addiction, a hedonic response is developed from lower than normal levels of dopamine (DA) in which the individual pursues drug-seeking behavior. The current accepted treatment methods for addiction are replacement drug therapies, group therapy, or individual counseling – the prior being associated with additional side-effects and an inability to overcome the hedonic response of the addiction. The aim of this study was to evaluate alternative and natural therapeutics that produce long-term potentiation (LTP) of the neuronal systems involved in order to overcome addiction with minimal to no side-effects. Using fast-scan cyclic voltammetry (FSCV), the effects of baicalein, a flavonoid isolated from the root of Sculletaria Baicalensis, and low-level laser therapy (LLLT) on DA release in the NAc core were evaluated in vitro and in vivo in Wistar rats. Local stimulation evoked in vitro demonstrated that baicalein administration (10, 50, 100 uM) 30 minutes prior to 80 mM ethanol attenuated the DA inhibition of ethanol. DA signals were evoked in vivo in the core of the NAc by electrical stimulation of the medial forebrain bundle (MFB) at the level of the lateral hypothalamus (60 Hz, 60 pulses) in isoflurane anesthetized rats. Both the intraperitoneal (IP) administration of baicalein (1.0 mg/kg) and the administration of LLLT (25 Hz, 630 nm) 30 minutes prior to ethanol (2.0 g/kg) administration IP attenuated the DA inhibition of ethanol. These findings suggest that baicalein and LLLT may prove as effective clinical therapies for addiction.

Kayla Bevard, Brigham Young University Microbiology and Molecular Biology Metabolic diseases, such as obesity and diabetes, are endemic in American society. Mutations in PAS kinase, a recently discovered sensory protein kinase, have been shown to cause Maturity Onset Diabetes of the Young (MODY) in humans (Semplici et al., 2011). In addition, PAS kinase deficient mice, when placed on a high fat diet, display phenotypes related to diabetes including resistance to weight gain, insulin insensitivity and triglyceride accumulation (Hao et al., 2007). PAS kinase consists of a sensory PAS domain that binds to and inhibits the kinase domain (Amezcua et al., 2002). Our model for PAS kinase activation involves the generation of a small molecule ligand that binds to the PAS domain and relieves this inhibition. We are currently engaged in several yeast genetic screens which will identify regions in the full length PAS kinase protein that are essential for either PAS kinase activation or for binding of its substrates. The first screen is based on the ability of PAS kinase, when overexpressed, to rescue a temperature-sensitive mutation in Tor2, the tor2(ts). We have isolated both point mutations and truncations in PAS kinase which alleviate the tor2(ts). These mutations solidify our model for PAS domain inhibition and will identify novel regions involved in PAS kinase regulation. Our second screen uses the yeast 2-hybrid to select for both point mutations and truncations that increase the affinity of PAS kinase for its substrate, Pbp1. These mutations will help identify key regions of PAS kinase utilized in substrate recognition. Finally, we will compare the regions affected by our mutations with the regions of PAS kinase that we have found to be well-conserved throughout evolution. Analysis of these specific genetic regions will help elucidate the molecular mechanisms involved in the regulation and function of PAS kinase, a key player in the development of metabolic disease.

Anindita Ravindran, University of Utah Exercise and Sport Science Obesity predisposes individuals with Type II Diabetes to cardiovascular complications such as impaired blood vessel function. Due to the elevation of free fatty acids (FFAs) in obese individuals, ceramide, a lipid metabolite, accumulates and might contribute to the inability of a blood vessel to constrict or relax appropriately. Vessel dysfunction is partly caused by the inability of the endothelium, the innermost protective lining of blood vessels, to synthesize and release nitric oxide (NO). Our data indicate that ceramide impairs endothelial NO synthase (eNOS), the enzyme that synthesizes NO. In order to study mechanisms by which ceramide might impair eNOS, it is important to measure cellular ceramide production in response to pharmacological and genetic manipulations. Previously we used P-32 radioactive assays to measure ceramide accumulation. However, the use of radioactivity is expensive, potentially hazardous, and waste disposal is an environmental concern. Therefore, I sought to import a less harmful, more cost effective, yet accurate technique of measuring ceramide production by immunofluorescence (IF). IF allows ceramide to be tagged with a primary antibody which can be detected by a secondary antibody conjugated with a fluorescent dye. I have observed that 250, 500, and 750 uM palmitate (pal) incubation for 3 h increases (p<0.05) endothelial cell ceramide accrual in a dose-dependent manner. Further, a FFA-independent method to alter ceramide accrual i.e., 3 h incubation of cells with N-oleoylethanolamine, also elevates (p<0.05) ceramide production. Importantly, I have shown that 500 uM palmitate-induced ceramide accrual can be prevented (p<0.05) by two structurally dissimilar inhibitors (10 uM myriocin, 1mM L-cycloserine) of the rate-limiting enzyme responsible for ceramide biosynthesis i.e., serine palmitoyl transferase (SPT). None of these inhibitors impairs cell viability. These data indicate that IF is an accurate and reproducible method whereby ceramide accrual can be quantified in endothelial cell systems.

Jason Chen, University of Utah Biology Both human language and birdsong are learned sensorimotor processes that require exposure to adult communication signals in order to be reproduced. The social aspect of the development of these vocal signals is distinctive because interaction is not required for the development of vocal signals in other organisms. Human infants isolated from interaction will not learn to speak normally. The need for interaction is also characteristic of the ontogeny of vocal signals in the zebra finch (Taeniopygia guttata), but only zebra finch males develop vocal signals, in the form of song. Juveniles raised in isolation will develop highly atypical song characterized by prolonged, monotonic syllables and irregular rhythms. When juveniles are tutored by these isolated birds in one-on-one conditions their song drifts towards normally raised birds. It is unclear how tutoring, as a social interaction, influences this observation. We hypothesized that different social experience influences birdsong. We predicted the song of juveniles raised in isolation with an experienced female to be closer to normal song than that of those raised in isolated sibling groups, or those raised in isolation. Current data suggest that the syntax and temporal features of song from juveniles raised in isolation with experienced females is indeed closer to normal song than that of isolated juveniles.

Harsh Kansagra, Southern Utah University Biology Anthoxanthum hirtum is a native grass with many traditional and modern uses, including human medicinal benefits. Populations are found locally in Utah, but at higher elevations, usually above 2500 m. Indigenous people used native sweetgrass in a variety of ways, including medicinally, as ceremonial incense, and in basketry. The active compound that elicits the sweet fragrance of the grass is produced by coumarin, a secondary metabolite used today both medicinally and commercially. Plants most often produce secondary metabolites, or essential oils, as a defense against pathogens, but these antimicrobial properties have not been investigated in A. hirtum. Our research used the Kirby-Bauer disk diffusion test to determine if closely related commercial diploid and polyploid sweetgrass strains (Anthoxanthum odoratum), as well as plants from native A. hirtum populations, produce zones of inhibition when tested against associated soil bacteria and fungi. Results of our research showed all species tested produced inhibition zones, but zone size varied in response to the secondary metabolites produced by each plant type. Despite this variation, these data suggest components of the essential oils may have antimicrobial properties. Results of this study increase our understanding of the antimicrobial properties of secondary metabolites produced by A. hirtum as well as the essential oils produced by commercial diploid and polyploid strains. Future studies will focus on identifying the chemical composition of each extract as well as the specific bacterial and fungal species associated with each plant.

Diego Cordero, University of Utah Biology Many zygotes and spores of brown algae are photosensitive and establish a developmental axis in accordance with directional light cues. Ectocarpus siliculosus is being advanced as a genetic and genomic model organism for investigating brown alga development, and this report investigates photopolarization of the growth axis of mitospores. When exposed to unidirectional light, mitospores photopolarized and established a growth axis such that germination was preferentially localized to the shaded hemisphere of the spore body. The roles of the microtubule cytoskeleton and endomembrane cycling in the photopolarization process were investigated using pharmacological agents. Disruption of microtubule dynamics progressively reduced the percentage of mitospores that photopolarized, while inhibition of vesicle secretion blocked photopolarization nearly completely. Chronic treatment with these pharmacological agents severely affected algal morphogenesis. Microtubules in mitospores and algal filaments were imaged by confocal microscopy. Mitospores contained a radial microtubule array emanating from a centrosome associated with the nuclear envelope. At germination, the radial array gradually transitioned into a longitudinal array with microtubules extending into the emerging apex. At mitosis, spindles were aligned with the growth axis of cylindrical cells in the filament, and the division plane bisected the spindle axis. These studies demonstrate that dynamic membrane cycling and microtubule assembly play fundamental roles in photopolarization, and provide a foundation for future genetic and genomic investigations of this important developmental process.

Travis Wood, Southern Utah University Biology Speckled dace, Rhinichthys osculus, is a small minnow that occupies river systems in Utah, Arizona, and Nevada. These minnows prefer clean, faster-moving water with plenty of cover such as rocks or undercuts. The invasive crayfish Orconectes virilis inhabits areas where the Virgin spinedace is found and has a similar diet being an opportunistic feeder. I tested the hypothesis that the natural behavior of Virgin spinedace will be altered due to the presence of crayfish. I predicted that the spinedace would avoid the crayfish and stay out in the open exposing them to a higher risk of predation, since crayfish are a more aggressive organism and will try and eat virtually anything they come into contact with. The experimental trials were conducted (N=24) in Quail Creek, Utah. Large plastic bins with the front and back replaced with screen were used to allow water flow through our experimental chambers. Each bin was placed in the creek and had rocks, and an undercut bank to resemble the native habitat. Controls consisted of placing isolated Virgin spinedace in one of the bins to observe its behavior. In both controls and experimental trials, either 2 spinedace, or 5+ spinedace were added. In experimental trials, I introduced 2 crayfish into the experimental chambers with the Virgin spinedace and allowed them to acclimate for 10 minutes. After the 10 minute acclimation period, behavioral observations were made. Results indicated that when 2 spinedace were present along with the crayfish, they would stay out in the open and the crayfish would occupy the cover or undercut. When 5+ spinedace were present with crayfish, the spinedace displayed a mobbing or “strength in numbers” behavior where they would occupy the undercut and either clog it up with their presence so the crayfish would leave, or mob the crayfish to make them leave. This behavior was opposite of what I predicted would happen. Since this mobbing or “strength in numbers” behavior was not observed with the samples of just 2 spinedace, this may be a behavioral adaptation resulting from the presence crayfish. Further research can be done on the behavioral effects of various fish species in the presence of introduced or invasive species to determine if this behavior is seen in other fishes.

Sarah Miller, Southern Utah University Agriculture and Nutrition Science The high intake of fat in the average American diet is a growing concern to the nutritional community. High levels of total fat intake increase cardiovascular disease (CVD) which is the number one killer in America. The successful development of low fat recipes that can be accessed and produced by the general population is valuable to the fight against obesity and CVD. If acceptable products can be made with lower fat content then it is possible to lower the fat intake in a person’s diet. This study is designed to test the acceptability of low-fat desserts using every day food products available for home use as fat substitutes. Four popular “name-brand” recipes were altered by removing 55-73% of the fat and replacing it with a common food item, either pureed white or pinto beans, applesauce or yogurt. Southern Utah University students were recruited via flyers, in class announcements, and word of mouth. Participants (n=56) were asked to taste and evaluate the four products. Afterwards they completed a short survey about their knowledge of low-fat food substitutes and acceptability of the products. Results and conclusions forthcoming.

Sarah Miller, Southern Utah University Biology As humans, we have the ability to change our diets if we are deficient in any specific nutrient. Many animals have the ability to change their diet if they are deficient in nutrients. However, for many carnivores, it is generally thought that a prey item will have the same nutritional composition as the predator body composition. Carnivores are limited nutritionally in their diet. This means that they only get nutrients from the prey that they are able to locate to consume. This study is investigating prey choice of invertebrate predators (wolf spiders) when their prey item has been altered to be deficient in one specific nutrient (lipid or protein). I predict that the spiders will be able to choose the correct prey item for the nutrient in which they are deficient. The total weight of flies eaten in each treatment will be measured to determine if wolf spiders select prey items based on the nutrient content of the prey. Results will be analyzed using a nested ANOVA.

Joshua Horns, University of Utah Biology There are numerous arresting differences between the hands of humans and those of chimps, principally among them the shortening of metacarpals 2-5, the relative increase in length of the thumb, and the development of separate musculature controlling flexion of the thumb. In addition to improving manual dexterity, these changes allow humans, in contrast to chimps and other apes, to make a buttressed fist with the phalanges pressed against the central palm and the thumb adducted onto the dorsal surface of the phalanges. We believe this hand posture greatly reduces the strain experienced by a fully formed fist when striking with force, thereby rendering the human hand as a more effective weapon. We tested this idea by manipulating the tendons in a cadaver arm to induce the hand to form into buttressed and non-buttressed conformations, and then tested each conformation by having the hand strike a hanging instrumented mass to measure the force of each strike. Additionally, the 2nd metacarpal of the hand was fitted with a strain gauge so that strain in the bone could be compared to the force of the strikes. The results of these tests showed that there was a significant drop in strain in the buttressed fist (in comparison to the non-buttressed) for a given force.

Alexa Provancha, University of Utah Exercise and Sport Science Impaired endothelium-dependent vasodilation has been associated with various cardiovascular diseases, including heart failure, is linked to oxidative stress, and persists despite heart transplantation (HTx). PURPOSE: This study aimed to determine if changes in vascular function following HTx can be assessed using incremental handgrip (HG) exercises to induce nitric oxide-dependent vasodilation of the brachial artery. Furthermore, the efficacy of an acute oral antioxidant cocktail (AOC) to reduce oxidative stress and improve vascular function in this population will be assessed. METHODS: 31 HTx recipients (14 years post-HTx) and 10 healthy age-matched controls were given either and AOC (Vitamin C, E, and alpha-lipoic acid) or placebo (PL; randomized for the subjects’ two visits. Measurements of the brachial arterial blood velocity and vessel diameter were performed during three absolute workloads (4, 8, and 12 kg) of HG exercise using ultrasound Doppler. RESULTS: Maximal vasodilation during PL HG exercise was not different between the controls (8.2 ± 1.5%), and recent (< 3 years post) HTx group (8.5 ± 1.2%), but the 5-10 years post-HTx groups had a tendency to be lower (6.5 ± 1.9%). The > 14 years post-HTx group recipients (5.2 ± 1.9%) were significantly attenuated compared to both the controls and the recent (< 3 years post) HTx recipients. CONCLUSION: The study supports the use of HG exercise as an assessment of vascular function in a patient population with known cardiovascular risk. Additionally, these results suggest that vascular function is similar between controls and early HTx recipients but declines as time passes following surgery despite normalized cardiac function.

Dillon Monroe, Southern Utah University Biology When an area is developed no part of that habitat is left unaffected. Urbanization creates many problems for animals by creating physical barriers, fragmenting habitat, introducing completely new habitat, and introducing species. Exotic species are often better competitors for resources and often competitively exclude native species, resulting in population declines and ecosystem disruption. Despite the negative effects of urbanization some species are able to adapt and survive in urban ecosystems. One such species is the wandering garter snake (Thamnophis elegans vagrans). This species is described as a generalist in habitat selection and is often seen in urban communities basking on sidewalks and pavement, living in gardens, and feeding on rodents, insects, and other species. One unique characteristic of this genus is that prey preference has been observed as being a genetic trait and possesses variation, and as such can be modified via natural selection. For my research project I looked at whether prey preference of T. elegans vagrans differs between snakes from three different locations with varying levels of human development. From these three locations in Utah, Tropic reservoir, Monroe (Sevier River), and Utah Lake, we obtained a total of 14 snakes and found that in this group of snakes there is no correlation of prey preference in snakes to level of development of the habitat.

Andrew Jo, University of Utah Ophthalmology and Visual Sciences All cells, including retinal neurons and glia, must sense and adapt to physical changes in their local environment (e.g. changes in osmotic pressure). Osmotic water flux can cause aberrant cell volume changes, which can contribute to tissue damage, edema, and neuronal hyperexcitability and excitotoxicity. We hypothesized that force-sensitive proteins enable retinal cells to monitor their physical form (e.g. volume) and help maintain homeostasis by regulating cell volume. To test this, we first investigated the properties of cell morphology when cells were bathed in solutions with different tonicities. Under these conditions, we measured changes in cell volume due to osmosis in retinal ganglion cells (RGCs) and Müller glia. We found RGCs were unable to actively adjust their volume, whereas Müller glia reduced their swollen volume in the continued presence of hypotonicity. The regulation of cellular volume often involves calcium signals. We tested whether calcium plays a role in the regulation of retinal cell volume. Free calcium within the cells was sequestered using cytosolic BAPTA, which decreased the extent of hypotonic swelling. This demonstrates that calcium elevations increase the extent of cell swelling. Because cell volume was dependent on calcium, which was elevated by membrane stretch, we hypothesized that the osmosensitive cation channel TRPV4 would transduce osmotic pressure and contribute to cell volume regulation. In an experiment using a selective TRPV4 inhibitor, the extent of hypotonic-induced swelling was reduced. Thus, the opening of TRPV4 leads to a calcium influx that exacerbated cellular swelling. In addition, we tested the idea that TRPV1 cation channel is involved in responses to osmotic stimuli. In an experiment using a selective TRPV1 antagonist, preliminary results show that the extent of hypotonic-induced swelling decreased. This signals that TRPV1 may have a role in volume regulation in retinal neurons and glia. Thus, inhibition of these force-sensitive protein channels might alleviate the deleterious effects of volume changes in pathological contexts. Our findings therefore have implications for our understanding of retinal mechanotransduction and osmoregulation as well as provide a mechanistic framework for developing new therapeutic strategies aimed at blinding conditions that involve mechanical stress and cellular morphology.

Makenzie Hawkins, University of Utah Nutrition Cardiovascular disease (CVD) is the leading cause of death in the United States. Gamma-tocopherol (GTC) has been studied in its relation to hypertension and CVD. GTC has protective effects on the endothelium of arteries; the endothelium is in part responsible for regulating arterial relaxation and reducing blood pressure (BP). Diabetic mice (DB) were chosen for this study because of the associated co-morbidities of hypertension and excess body weight. Eight-week-old mice were divided into 3 separate groups: wild-type fed control (CON) diets (n=12), DB fed CON diets (n=12), and DB fed GTC supplemented diets (0.1%, n=12). BP was measured every week for 8 wk. Body weights were measured every wk and morphometric values were measured at the end of the 8-wk period. There was no statistically significant (P<0.05) difference in systolic and diastolic BP between DB-GTC and DB-CON. There was also no statistically significant (P<0.05) difference in heart rate between DB-GTC and DB-CON. After 8 wk, DB-GTC mice had lower (P<0.05) body weight versus the DB-CON group. In conclusion, a GTC supplemented diet did not result in a change in BP. However, GTC may have blunted weight gain and adipose tissue mass in DB-GTC compared to DB-CON. We speculate this may be due to a reduction of food consumption during the experimental period.

Viktor Jiracek, University of Utah Biology The nervous system is responsible for cognition, memory, and motor function. Neurons communicate with each other at intercellular connections called synapses. It is at these locations that vesicles fuse with the plasma membrane and release their contents into the space between the neurons (synaptic cleft). SNARE proteins (synaptobrevin, syntaxin and SNAP-25) facilitate synaptic vesicle fusion by winding together in a four-helix bundle forcing the mixture of opposing membranes. After exocytosis, the cell regenerates vesicles by a process called endocytosis. This process involves the budding of membrane back into the cytoplasm re-forming a functional synaptic vesicle. This progression from vesicle fusion to internalization is termed the “synaptic vesicle cycle” and is coordinated by a long list of molecular players. The SNARE proteins are classically considered to be unique to exocytosis. However, preliminary results from Erik Jorgensen’s lab have implicated SNAP-25 in endocytosis. Although we have evidence that SNAP-25 is required for endocytosis, the molecular mechanism is completely unknown. In this proposal we use forward genetics in the model organism C. elegans to identify novel protein interactions required for SNAP-25 mediated endocytosis. We have designed three suppressor screens that are predicted to target the role of SNAP-25 in exocytosis, endocytosis and general function. The first two screens use crippled forms of SNAP-25 (hypomorphs) while the last screen uses a deletion allele of the SNAP-25 gene (null mutation). With these screens we hope to precisely identify the molecular players involved in SNAP-25 mediated endocytosis versus exocytosis.

Heather Shumway, Southern Utah University Biology Glossiphoniids are jawless leeches that feed using a proboscis, which they use to impale prey so that they can feed on their prey’s blood and tissues. This study focuses on two different species, Glossiphonia complanata and Helobdella stagnalis. Helobdella stagnalis feed on a variety of prey, including bloodworms and freshwater snails, often feeding together in large groups. When a prey item is placed in the container, H. stagnalis immediately begin smelling the water and moving toward the prey. After the first H. stagnalis makes contact and secures prey, other individuals within the group will move to that area, often passing prey along the way, to attach to the same prey item. Once a number of individuals have started feeding, the prey is often broken into smaller pieces and individuals can then begin feeding on various sized pieces. This is in contrast to members of the species Glossiphonia complanata, who appear to be selective with their prey, and have been observed going up to 4 weeks without feeding, even in the presence of their non-preferred prey (bloodworms). When preferred prey (freshwater snails) were placed in the container, unfed G. complanata did not immediately begin smelling the water or initiate hunting. Unlike H. stagnalis which feed in groups, G. complanata appear to prefer feeding alone, and have even exhibited competitive behaviors.

Thomas Wallace, University of Utah Pharmaceutics and Pharmaceutical Chemistry The tumor suppressor p53 is mutated in more than 50% of all cancers, while the majority of remaining cancers contain mislocalized p53(1). p53 is part of a network of cellular pathways that regulate growth, cell cycle arrest, and clearly delineated apoptotic pathways(2). Restoring the function of p53 can be seen as an ultimate cancer treatment. Restoring p53 would activate an already existing cell pathway that prevents cells from becoming cancerous and cause cancer cells to go through apoptosis. Playing a role in tumor suppression has made p53 an attractive target for gene therapy. However, despite the potential of p53 as a powerful treatment, it is limited by the dominant-negative effect of dysfunctional mutant p53. This effect imposes one of the greatest limits on the effectiveness of a p53 based treatment. This project is focused on bypassing the dominant-negative effect of dysfunctional p53 over exogenous functional p53. The attempted solution was substitution of the p53 binding domain with a different but structurally analogous coiled-coil, based on a modified Breakpoint cluster region (Bcr) protein. By doing this, the dominant-negative effect of mutant p53 may be bypassed. The purpose of this project has been to synthesis and test rationally modified forms of p53 with modified Bcr coil that are introduced into cells via a plasmid to restore cellular p53 activity. In vivo cell tests have already shown the effectiveness of these constructs at causing higher rates of cell death in cancer cells and constructs are currently being refined to carry forward to xenograft model animal trails. The ultimate goal is to develop a treatment for human cancer patients where modified p53 will selectively cause apoptosis in cancer cells.

Kandrie MyIroie, University of Utah Human Genetics In C. elegans, sperm activation is the process through which sperm become motile. Genes that regulate sperm motility are being identified through a genetic screen. In this screen, swm-1 mutants that have sperm that activate too early are mutagenized, and genes regulating sperm motility can be found when the sperm of mutant worms no longer activated too early. Two genes have already been identified, try-5 and snf-10, and these can help identify new mutations. By selecting specific strains with sperm activation defects, the mutations causing the defect can be characterized. To sort through the many mutants, complementation tests to identify them as a new gene, or a new allele of try-5, snf-10 are being done. Selectively crossing an unknown mutant strain to a strain with a known mutation in try-5 or snf-10 will help identify the unknown mutation. If the progeny of the cross have activated sperm then the two strains complement each other. In this case the alleles must be in different genes and the unknown mutation from that cross has not been identified. If the progeny instead has non-activated sperm then the mutations failed to complement and the unknown mutation is allelic to the mutation it was crossed with. One new allele of try-5 and of snf-10 has been found. It is suspected that the other strains contain alleles of new genes not previously identified. Further complementation tests will be done to test this theory.

Ashley Stengel, University of Utah Biology Agricultural herbivores, such as cattle, often encounter plants containing toxins. One class of toxins, tannins, bind to proteins and inhibit digestive enzymes from acting to liberate nutrients. In this way, tannins prevent optimal absorption of nutrients. To overcome this challenge, some herbivores host bacteria with the ability to degrade tannins. Currently, there is a push to find novel microbes capable of aiding animals in detoxifying these compounds. Therefore, we aimed to isolate tannin-degrading bacteria from the desert woodrat (Neotoma lepida), a wild, herbivorous rodent that feeds largely on a tannin-rich shrub, creosote bush (Larrea tridentata). Woodrat feces were cultured on tannin-treated media, and bacteria capable of degrading tannins were further characterized with DNA sequencing. Results revealed that from 9 isolates, three species of tannin-degrading bacteria were present: Enterococcus faecalis, Bacillus subtilis, and Escherichia coli. Further characterization was performed through measurement of tannase activity. The activity of each isolate varied significantly between bacterial species as well as within a species. Ultimately, I hypothesize that these tannin-degrading bacteria facilitate the ingestion of tannin-rich plants by woodrats. Additionally, this research exemplifies how wild herbivores, such as the desert woodrat, provide an essential source of tannin-degrading microbes that could be introduced into domestic herbivores in order to improve agricultural practices.

Lee Leavitt, University of Utah Biology Chronic methamphetamine (METH) abuse leads to structural and functional damage in the brain, which likely contributes to cognitive and behavioral dysfunction. Recent data suggest an association between METH abuse and impaired inhibitory control over behavior; that is an impaired ability to inhibit inappropriate actions or thoughts. However, the extent to which METH-induced neurotoxicity is responsible for such impairment remains to be determined. Previously, we reported that rats with METH-induced partial dopamine (DA) and serotonin (5-HT) loss in striatum and prefrontal cortex (PFC) exhibited impaired response inhibition in the form of perseverative responding. Furthermore, levels of serotonin transporter (SERT) binding in PFC correlated with perseverative responding. Herein, we have examined another dimension of response inhibition impulsive action, which is an inability to withhold a “pre-potent” response in rats with METH-induced neurotoxicity. Rats were trained to perform a stop-signal task (SST). Once rats achieved stable responding (>80% correct response) on both “Go” and “Stop” trials, they were treated with saline (0.9%), a neurotoxic regimen of METH (4 x 10 mg/kg, 2-hr intervals, s.c.) under normal ambient temperature conditions (“neurotoxic” METH group) or the METH regimen under cooling conditions (“normothermic” METH group). One week after the treatment, rats were again tested on the SST. After behavioral tests were done, animals were sacrificed and brains removed for determination of monoamine loss. The results showed: 1) the “neurotoxic” METH-, but not the “normothermic” METH or saline-treated rats, showed a 40-60% loss of SERT and dopamine transporter (DAT) binding in PFC and striatum; 2) the “neurotoxic” METH group showed normal behavioral performance in “Go” trials of the SST relative to the “normothermic” METH group or saline controls; 3) the “neurotoxic” METH group exhibited impaired withholding of a “pre-potent” response, as reflected by increased numbers of errors on the “Stop” trials of the SST. Overall, these data suggest that impaired inhibitory control over behavior (i.e., increased impulsive action) can arise as a consequence of METH-induced neurotoxicity to central dopamine and serotonin systems. Supported by NIH grant DA 024036

Tyler Shimko, University of Utah Biology Differences in the genomes of organisms control an organism’s ability to deal with and adapt to environmental stresses. In this project, two strain isolates of the nematode Caenorhabditis elegans were analyzed using high-throughput assays measuring growth and offspring production to determine the genes that confer a greater resistance to the herbicide paraquat. Paraquat acts by interfering with electron transport mechanisms within the cells of living organisms. This mechanism not only allows it to act as an effective herbicide, but also causes it to pose a considerable risk to the health of animals, including livestock and humans. Using statistical genetics, regions of the genome were identified that are likely responsible for differences in growth rate and fecundity observed in the two strain isolates when grown in paraquat. Near-isogenic lines and extra-chromosomal arrays were then created to isolate these portions of the genome in a control genetic background. This approach allowed us to be able to attribute any differences in the two traits to the genes contained within the intervals. After analyzing the body size data, representing the growth of the animals over 72 hours, we were able to draw a preliminary conclusion that an interval on chromosome V may have a small but significant effect on growth determination. As a result of this project, a specific interval was identified that may be responsible for a greater growth rate, three near-isogenic lines were created, and 34 extra-chromosomal arrays were generated. This work will be used in the future to identify the gene(s) responsible for the greater growth rate and fecundity observed in some animals exposed to the herbicide paraquat. These results will allow us to draw conclusions about the roles that these genes, and others like them, play in an organism’s ability to cope with environmental stresses.

Heidi Montgomery, University of Utah Biology Parasites are arguably the most diverse group of organisms on the planet. One key factor in determining patterns of parasite biodiversity is the number of host species a parasite can infect, or its host specificity. However, surprisingly very little is known about the evolution of host specificity. Here I use domesticated pigeons (Columba livia) and their ectoparasitic feather lice (Columbicola columbae) to demonstrate how parasite body size influences host use. The current study has two main goals: 1) to use different sized hosts to experimentally evolve louse body size, and 2) to determine how parasite body size effects host specificity. Four breeds of domesticated pigeons were infested with parasitic feather lice. After roughly six generations, mean louse body size changed with respect to host body size, with the largest pigeons having the largest lice. Interestingly, the smallest pigeons had larger lice as well. However, this increase in louse size appears to be the result of a change in microhabitat use, with lice shifting from predominantly the wings and tail to the head. This study is one of the first to experimentally demonstrate how changes in parasite morphology and behavior influence the evolution of host specificity.

Sam Hawkins, Utah State University Biological Engineering Colorectal cancer (CRC) is one of the most frequently diagnosed cancers in women and men. It is often treatable if caught early. However, tumors may metastasize which can result in a poor prognosis. A better understanding of the tumorigenesis and evolution of metastatic tumors in CRC patients could lead to earlier diagnosis, pre-emptive screening, and a better outcome. Copy number analysis of primary tumor tissue has revealed genes associated with colon cancers, but a comparison between primary and metastatic tumors has never been done. Normal tissue, primary tumor tissue, and metastatic tumor tissue was collected from twentyfive individuals. Copy number alterations were determined by microarray data generated from Molecular Inversion Probe (MIP) technology (Affymetrix, Inc.) for copy number analysis using Nexus software (BioDiscovery, Inc.). Metastatic tumor samples show a greater rate of copy number alterations (CNAs) from the primary tumors and even more alterations from normal tissue samples. Certain regions of the metastatic genome show high rates of CNAs whereas the primary tumor genome does not. These areas are key regions for potential understanding into the molecular origins of metastatic tumors. Understanding specific regions and genes with CNAs in metastatic tumor samples may lead to further research in cancer genetics and possible target areas for pharmaceutical testing.

Makda Gebre, Utah State University Biology The first pandemic of the 21st century was the influenza A (H1N1pdm09) virus that originated in Mexico and killed 12,000 people within the United States. It now circulates as a seasonal influenza virus causing mild symptoms in most but still killing some susceptible individuals. Influenza A (H1N1pdm09) virus leads to acute respiratory distress syndrome (ARDS) in high risk patients and has a mortality rate of 40-50% in those patients. ARDS patients usually die before any intervention since there is no clinical evidence that can be used to detect it. To detect ARDS early in disease progression, we need to search and develop biomarkers that can be tested in the patient’s blood or fluids. The three biomarkers tested as potential early disease indicators in this project included: C-reactive protein (CRP), serum amyloid A (SAA) and Transferrin. To test these biomarkers, mice were infected with the influenza A H1N1pdm09 virus and serum was collected from the mice at different time points. Serum collected was tested for amounts of CRP, SAA and Transferrin using an enzyme-linked immunosorbent assay (ELISA) and spectrophotometer. Control mice were mock infected and also tested for the biomarkers for comparison.

Thomas Anderson, Utah State University Biology Pradimicin, a small molecule produced by the soil bacterium Actinomadura hibisica, is a promising candidate as a combined antifungal/antiviral therapeutic. It is active against a broad-spectrum of opportunistic, pathogenic fungi, interferes with the replication of influenza virus, and inhibits the reproduction of HIV-1. Toxicity and solubility problems have hindered past efforts to develop pradimicin as a therapeutic. Our research focuses on elucidating the bio-synthetic pathway of pradimicin in order to design and chemoenzymatically create pradimicin structural analogs with improved solubility and activity, and less toxicity. Several enzymes in pradimicin biosynthesis have been identified. We intend to characterize one of the key enzymes, PdmS, a putative glycosyltransferase, and to manipulate its gene to create novel, more efficacious pradimicin analogs. This project is funded for three years by the NIH NIAID (3 years). Methods: A gene knockout experiment was used to determine the role of PdmS in pradimicin production. Bio-synthetic precursors of pradimicin were subjected to bio-transformations in E. coli with recombinant genes for PdmS and another glycosyltransferase, OleD, to generate analogs with new sugar attachments. Analogs of pradimicin will be screened for bio-activity using standard microbroth dilution assay techniques. Confirmed results: The enzyme PdmS was identified and characterized as a glycosyltransferase. Expected results: newly created analogs of pradimicin exhibited minimal inhibitory concentrations of 10μg/mL against Candida albicans. Conclusion: Knockout of pdmS yielded the pradimicin aglycon, which confirmed the function of this glycosyltransferase and provides a start molecule for further structural modification to yield new analogs for bioactivity studies.

Emily Matis, Utah Valley University Exercise Science Introduction:

Monica Cervantes, Utah Valley University The central research question of this project was to determine if high-frequency ultrasound is sensitive to tissue pathology at the microscopic level. Previous studies on surgical specimens have shown that high-frequency ultrasound may be sensitive to a range of breast pathologies including fibroadenomas, atypical ductal hyperplasia, fibrocystic changes, and carcinomas. The ultrasonic parameters that were sensitive to pathology were the number of peaks (the peak density) of the first-order spectra of the waveforms (one forward Fourier transform), and the slope of the second-order spectra of the waveforms (two consecutive forward Fourier transforms). The ability to determine pathology rapidly and with minimal specimen preparation would make high-frequency ultrasound particularly well-suited for real-time use during cancer surgery to ensure all of the malignant tissue has been removed. The purpose of this research was to determine the sensitivity of the peak density and spectral slope to tissue microstructures other than those found in breast cancer. The results of this study would not only support the results from the breast cancer studies, but also extend those results to the detection of cancer and other diseases in a range of organs and tissues. The research methodology included the following steps. (1) Freshly excised bovine organs were obtained from a meat packaging facility, including the heart, liver, and kidney. (2) Specimens approximately 3x3x1 cm in size were dissected from the organs and tested immediately with ultrasound. (3) Both pitch-catch and pulse-echo waveforms were acquired from the samples. (4) The data were analyzed by determining the peak densities and spectral slopes. The results showed that the more heterogeneous tissues of the heart, the vascular structures (aorta, vena cava, etc.), displayed significantly higher peak densities than the muscle tissues. Similarly, the ureter, which has greater heterogeneities in its structure (larger and more varied), displayed significantly higher peak densities than the cortex and medulla tissues. No significant trends were observed for the liver tissue, or for the spectral slopes except for kidney medulla tissue. Heterogeneity and peak density in high-frequency ultrasonic spectra that may be useful for performing real-time pathology during cancer surgery.

David Gage, Utah State University Biological Engineering A new N-oxide was produced from 3-(N-Boc-aminomethyl)-5-bromopyridine by bioconversion with Cunninghamella echinulata var. elegans ATCC 9245, and its structure was established based on spectral data. The microbial N-oxidation is efficient and highly selective. The substrate was transformed into the product in 7 days.

Kat Combs, Utah State University Plants, Soils, and Climate Kentucky bluegrass (Poa pratensis L.) is a commonly used turfgrass species with many varieties being sold around the world. However, those varieties are very difficult to tell apart morphologically. Our objective was to use genetic markers (primers) to identify varieties, even if they are visually similar. This is valuable to the turfgrass industry for plant variety protection. We also wanted to use this data to explore the apomictic tendency (clonal reproduction) of the varieties. We collected leaf tissue from 24 Kentucky bluegrass varieties, extracted DNA, and sequenced portions using 29 EST and 21 genomic primers. This data was used to determine genetic relationships using a neighbor-joining dendrogram. Similarities of the genetic sequences from the varieties were estimated using the DICE coefficient. We found more polymorphisms in genomic primers than in EST primers with high variability between the varieties. Both types of primers were robust enough to distinguish varieties and that each variety was unique and genetically distinguishable. In addition, we discovered some varieties had large amounts of variation within a variety. This was unexpected due to the usual apomictic nature of the species. The markers resulting from our research will be available to the turfgrass industry.

Robert Bradford, Utah Valley University Biology Most organisms exhibit latitudinal gradients in diversity (i.e., taxonomic richness decreases as latitude increases). Few studies have sought latitudinal gradients in lichens, especially in the midlatitudes. Our primary questions were: 1) do lichens along the west coast of the United States show latitudinal gradients? 2) If so, what is the rate of change and does the level of taxonomic richness affect this rate? We hypothesized that lichens would show a reverse latitudinal gradient in the region, as has been documented for lichens elsewhere in the northern hemisphere, but at a considerably smaller scale. This study fills in the gap in our understanding of lichen latitudinal gradients over large areas of North America. It also functions as a baseline for future climate change and conservation efforts. Our study area is bound at the south by the California-Mexico border (32.331° N) and at the north by the Washington-Canada border (47.178° N), and extends inland from the coastline to the crest of the Sierra Nevada Mountains (116.083° W, at its eastern -most point). We divided the region into 218 roughly equal-area (cite) grid cells using GIS, each bordered with latitudinal and longitudinal lines. We derived a list of all vouchered lichen specimens in each grid cell using Consortium of North American Lichen Herbaria, an online database. The data were synonymized, and species, generic, and familial richness were calculated for each grid cell. We found no correlation (R2 = 0.2306) between latitude and species richness, using the raw vouchered data. What we did find was a strong correlation (R2=0.9069) between sample density and species richness. These results are biased by sample density and do not reflect what is naturally occurring. We hypothesize that we can get an unbiased estimate of richness with MaxEnt models. Using the georeferenced lichen distributions and related climate data, we constructed species distribution models of all species with five or more occurrences (990 species). In GIS, we projected all 990 distribution models and our 218 grid cells together to calculate species richness for each cell at various thresholds (i.e. likelihood of occurrence at 10%, 20%, 30%, etc.).