2013 Abstracts
Dynamic Microtubules and Endomembrane Cycling Contribute to Polarity Establishment and Early Development of Ectocarpus Mitospores
Diego Cordero, University of Utah Biology Many zygotes and spores of brown algae are photosensitive and establish a developmental axis in accordance with directional light cues. Ectocarpus siliculosus is being advanced as a genetic and genomic model organism for investigating brown alga development, and this report investigates photopolarization of the growth axis of mitospores. When exposed to unidirectional light, mitospores photopolarized and established a growth axis such that germination was preferentially localized to the shaded hemisphere of the spore body. The roles of the microtubule cytoskeleton and endomembrane cycling in the photopolarization process were investigated using pharmacological agents. Disruption of microtubule dynamics progressively reduced the percentage of mitospores that photopolarized, while inhibition of vesicle secretion blocked photopolarization nearly completely. Chronic treatment with these pharmacological agents severely affected algal morphogenesis. Microtubules in mitospores and algal filaments were imaged by confocal microscopy. Mitospores contained a radial microtubule array emanating from a centrosome associated with the nuclear envelope. At germination, the radial array gradually transitioned into a longitudinal array with microtubules extending into the emerging apex. At mitosis, spindles were aligned with the growth axis of cylindrical cells in the filament, and the division plane bisected the spindle axis. These studies demonstrate that dynamic membrane cycling and microtubule assembly play fundamental roles in photopolarization, and provide a foundation for future genetic and genomic investigations of this important developmental process.
Strength in Numbers: Behavioral Response of Speckled Dace
Travis Wood, Southern Utah University Biology Speckled dace, Rhinichthys osculus, is a small minnow that occupies river systems in Utah, Arizona, and Nevada. These minnows prefer clean, faster-moving water with plenty of cover such as rocks or undercuts. The invasive crayfish Orconectes virilis inhabits areas where the Virgin spinedace is found and has a similar diet being an opportunistic feeder. I tested the hypothesis that the natural behavior of Virgin spinedace will be altered due to the presence of crayfish. I predicted that the spinedace would avoid the crayfish and stay out in the open exposing them to a higher risk of predation, since crayfish are a more aggressive organism and will try and eat virtually anything they come into contact with. The experimental trials were conducted (N=24) in Quail Creek, Utah. Large plastic bins with the front and back replaced with screen were used to allow water flow through our experimental chambers. Each bin was placed in the creek and had rocks, and an undercut bank to resemble the native habitat. Controls consisted of placing isolated Virgin spinedace in one of the bins to observe its behavior. In both controls and experimental trials, either 2 spinedace, or 5+ spinedace were added. In experimental trials, I introduced 2 crayfish into the experimental chambers with the Virgin spinedace and allowed them to acclimate for 10 minutes. After the 10 minute acclimation period, behavioral observations were made. Results indicated that when 2 spinedace were present along with the crayfish, they would stay out in the open and the crayfish would occupy the cover or undercut. When 5+ spinedace were present with crayfish, the spinedace displayed a mobbing or “strength in numbers” behavior where they would occupy the undercut and either clog it up with their presence so the crayfish would leave, or mob the crayfish to make them leave. This behavior was opposite of what I predicted would happen. Since this mobbing or “strength in numbers” behavior was not observed with the samples of just 2 spinedace, this may be a behavioral adaptation resulting from the presence crayfish. Further research can be done on the behavioral effects of various fish species in the presence of introduced or invasive species to determine if this behavior is seen in other fishes.
Developing Recipes That Reduce The Fat Content In Baked Foods Where The Fat Substitutes Are Common Foods
Sarah Miller, Southern Utah University Agriculture and Nutrition Science The high intake of fat in the average American diet is a growing concern to the nutritional community. High levels of total fat intake increase cardiovascular disease (CVD) which is the number one killer in America. The successful development of low fat recipes that can be accessed and produced by the general population is valuable to the fight against obesity and CVD. If acceptable products can be made with lower fat content then it is possible to lower the fat intake in a person’s diet. This study is designed to test the acceptability of low-fat desserts using every day food products available for home use as fat substitutes. Four popular “name-brand” recipes were altered by removing 55-73% of the fat and replacing it with a common food item, either pureed white or pinto beans, applesauce or yogurt. Southern Utah University students were recruited via flyers, in class announcements, and word of mouth. Participants (n=56) were asked to taste and evaluate the four products. Afterwards they completed a short survey about their knowledge of low-fat food substitutes and acceptability of the products. Results and conclusions forthcoming.
Ability of Wolf Spider’s (Lycosidae) to Assess Their Nutritional Needs and Deficiencies
Sarah Miller, Southern Utah University Biology As humans, we have the ability to change our diets if we are deficient in any specific nutrient. Many animals have the ability to change their diet if they are deficient in nutrients. However, for many carnivores, it is generally thought that a prey item will have the same nutritional composition as the predator body composition. Carnivores are limited nutritionally in their diet. This means that they only get nutrients from the prey that they are able to locate to consume. This study is investigating prey choice of invertebrate predators (wolf spiders) when their prey item has been altered to be deficient in one specific nutrient (lipid or protein). I predict that the spiders will be able to choose the correct prey item for the nutrient in which they are deficient. The total weight of flies eaten in each treatment will be measured to determine if wolf spiders select prey items based on the nutrient content of the prey. Results will be analyzed using a nested ANOVA.
The Role of Aggression in the Evolution of the Human Hand
Joshua Horns, University of Utah Biology There are numerous arresting differences between the hands of humans and those of chimps, principally among them the shortening of metacarpals 2-5, the relative increase in length of the thumb, and the development of separate musculature controlling flexion of the thumb. In addition to improving manual dexterity, these changes allow humans, in contrast to chimps and other apes, to make a buttressed fist with the phalanges pressed against the central palm and the thumb adducted onto the dorsal surface of the phalanges. We believe this hand posture greatly reduces the strain experienced by a fully formed fist when striking with force, thereby rendering the human hand as a more effective weapon. We tested this idea by manipulating the tendons in a cadaver arm to induce the hand to form into buttressed and non-buttressed conformations, and then tested each conformation by having the hand strike a hanging instrumented mass to measure the force of each strike. Additionally, the 2nd metacarpal of the hand was fitted with a strain gauge so that strain in the bone could be compared to the force of the strikes. The results of these tests showed that there was a significant drop in strain in the buttressed fist (in comparison to the non-buttressed) for a given force.
Urbanization and its Effects on Prey Preference in Wandering Garter Snakes (Thamnophis elegans vagrant)
Dillon Monroe, Southern Utah University Biology When an area is developed no part of that habitat is left unaffected. Urbanization creates many problems for animals by creating physical barriers, fragmenting habitat, introducing completely new habitat, and introducing species. Exotic species are often better competitors for resources and often competitively exclude native species, resulting in population declines and ecosystem disruption. Despite the negative effects of urbanization some species are able to adapt and survive in urban ecosystems. One such species is the wandering garter snake (Thamnophis elegans vagrans). This species is described as a generalist in habitat selection and is often seen in urban communities basking on sidewalks and pavement, living in gardens, and feeding on rodents, insects, and other species. One unique characteristic of this genus is that prey preference has been observed as being a genetic trait and possesses variation, and as such can be modified via natural selection. For my research project I looked at whether prey preference of T. elegans vagrans differs between snakes from three different locations with varying levels of human development. From these three locations in Utah, Tropic reservoir, Monroe (Sevier River), and Utah Lake, we obtained a total of 14 snakes and found that in this group of snakes there is no correlation of prey preference in snakes to level of development of the habitat.
Analysis of the Degradation of Flavor Volatiles in Single Origin High Cacao
Adrian Scottorn, Salt Lake Community College Natural Sciences Chocolate has long been the favorite snack of many, and recent studies have shown multiple positive health benefits of eating dark chocolate in particular, encouraging production and consumption of very high cacao content bars. We have quantified some of the changes that occur to the amount of known flavor compounds contained in a locally made artisan chocolate. The bars we chose were 70% minimum single origin cacao made with as few other ingredients as possible. By first measuring the amount of known flavor compounds, then stressing the bars in various ways, we looked in to what really happens when a quality chocolate bar is mistreated.
Characterizing the Properties of Cell Volume Regulation in Retinal Neurons and Glia: A Role for the Mechanosensitive Cation Channel TRPV4
Andrew Jo, University of Utah Ophthalmology and Visual Sciences All cells, including retinal neurons and glia, must sense and adapt to physical changes in their local environment (e.g. changes in osmotic pressure). Osmotic water flux can cause aberrant cell volume changes, which can contribute to tissue damage, edema, and neuronal hyperexcitability and excitotoxicity. We hypothesized that force-sensitive proteins enable retinal cells to monitor their physical form (e.g. volume) and help maintain homeostasis by regulating cell volume. To test this, we first investigated the properties of cell morphology when cells were bathed in solutions with different tonicities. Under these conditions, we measured changes in cell volume due to osmosis in retinal ganglion cells (RGCs) and Müller glia. We found RGCs were unable to actively adjust their volume, whereas Müller glia reduced their swollen volume in the continued presence of hypotonicity. The regulation of cellular volume often involves calcium signals. We tested whether calcium plays a role in the regulation of retinal cell volume. Free calcium within the cells was sequestered using cytosolic BAPTA, which decreased the extent of hypotonic swelling. This demonstrates that calcium elevations increase the extent of cell swelling. Because cell volume was dependent on calcium, which was elevated by membrane stretch, we hypothesized that the osmosensitive cation channel TRPV4 would transduce osmotic pressure and contribute to cell volume regulation. In an experiment using a selective TRPV4 inhibitor, the extent of hypotonic-induced swelling was reduced. Thus, the opening of TRPV4 leads to a calcium influx that exacerbated cellular swelling. In addition, we tested the idea that TRPV1 cation channel is involved in responses to osmotic stimuli. In an experiment using a selective TRPV1 antagonist, preliminary results show that the extent of hypotonic-induced swelling decreased. This signals that TRPV1 may have a role in volume regulation in retinal neurons and glia. Thus, inhibition of these force-sensitive protein channels might alleviate the deleterious effects of volume changes in pathological contexts. Our findings therefore have implications for our understanding of retinal mechanotransduction and osmoregulation as well as provide a mechanistic framework for developing new therapeutic strategies aimed at blinding conditions that involve mechanical stress and cellular morphology.
Does Gamma-Tocopherol Lower Blood Pressure in Diabetic Mice?
Makenzie Hawkins, University of Utah Nutrition Cardiovascular disease (CVD) is the leading cause of death in the United States. Gamma-tocopherol (GTC) has been studied in its relation to hypertension and CVD. GTC has protective effects on the endothelium of arteries; the endothelium is in part responsible for regulating arterial relaxation and reducing blood pressure (BP). Diabetic mice (DB) were chosen for this study because of the associated co-morbidities of hypertension and excess body weight. Eight-week-old mice were divided into 3 separate groups: wild-type fed control (CON) diets (n=12), DB fed CON diets (n=12), and DB fed GTC supplemented diets (0.1%, n=12). BP was measured every week for 8 wk. Body weights were measured every wk and morphometric values were measured at the end of the 8-wk period. There was no statistically significant (P<0.05) difference in systolic and diastolic BP between DB-GTC and DB-CON. There was also no statistically significant (P<0.05) difference in heart rate between DB-GTC and DB-CON. After 8 wk, DB-GTC mice had lower (P<0.05) body weight versus the DB-CON group. In conclusion, a GTC supplemented diet did not result in a change in BP. However, GTC may have blunted weight gain and adipose tissue mass in DB-GTC compared to DB-CON. We speculate this may be due to a reduction of food consumption during the experimental period.
Anodic Stripping Voltammetric Analysis of Lead, Cadmium, and Copper in the Jordan River, Utah
Chris Thurman, Salt Lake Community College Natural Sciences This project is an ongoing multifaceted investigation of the Jordan River. The study’s intent is to monitor and asses the overall condition of the river from Utah Lake to the Great Salt Lake. Samples of river and pond water, typical vegetation such as cattails, and some soils have been collected with reference to sites along the river that may serve as sites for the introduction of waste and other contaminants. These samples have so far been examined with respect to temperature and dissolved oxygen at the sampling point. Two river samples, one pond sample, and plant sample have been analyzed by ICP-MS for 20 different metals. The levels of Lead, Cadmium, and Copper have been determined by anodic stripping voltammetry at the ppb level in a variety of other water samples from the river. The results of these measurements are disused and presented Geospatially.
Analysis of Dental Parameters, Fluoride and pH, in Utah’s Waters, Drinks, and Foods
Lacie Cates, Salt Lake Community College Natural Sciences According to the Salt Lake Valley Health Department 1 the optimal level of fluoride is 0 .7 to 1.2 ppm in drinking water. Also acidity of many foods and drinks leads to tooth decay. In 2003 the State of Utah started adding fluoride to tap water. Then in 2008 Utah counties voted on addition of fluoride resulting in a variation of water treatment from county to county. At present the state has about 50% of the population receiving fluoride treated water with the aim of providing the 1 ppm fluoride level. It has also been reported that some counties are considering cutting back to about 0.7 ppm 2 . This study examined the pH and fluoride content of water samples from the major population counties in Utah. Fluoride levels ranged from 0.08 – 0.92 ppm. The pH values for these samples ranged from 6.26 -8.08. A further study of the fluoride levels and pH in bottled water and other drinks and foods such as fruit and cheese was conducted because many people in Utah do not drink tap water, particularly in regions of high water hardness or areas where taste and / or odor can be off putting. The pH values ranged from 2 to 8. The most acidic being colas and citrus based drinks and foods. The fluoride values varied from 0.03 to 0.47 ppm.
SNAP-25 Neurotransmission in C. elegans
Viktor Jiracek, University of Utah Biology The nervous system is responsible for cognition, memory, and motor function. Neurons communicate with each other at intercellular connections called synapses. It is at these locations that vesicles fuse with the plasma membrane and release their contents into the space between the neurons (synaptic cleft). SNARE proteins (synaptobrevin, syntaxin and SNAP-25) facilitate synaptic vesicle fusion by winding together in a four-helix bundle forcing the mixture of opposing membranes. After exocytosis, the cell regenerates vesicles by a process called endocytosis. This process involves the budding of membrane back into the cytoplasm re-forming a functional synaptic vesicle. This progression from vesicle fusion to internalization is termed the “synaptic vesicle cycle” and is coordinated by a long list of molecular players. The SNARE proteins are classically considered to be unique to exocytosis. However, preliminary results from Erik Jorgensen’s lab have implicated SNAP-25 in endocytosis. Although we have evidence that SNAP-25 is required for endocytosis, the molecular mechanism is completely unknown. In this proposal we use forward genetics in the model organism C. elegans to identify novel protein interactions required for SNAP-25 mediated endocytosis. We have designed three suppressor screens that are predicted to target the role of SNAP-25 in exocytosis, endocytosis and general function. The first two screens use crippled forms of SNAP-25 (hypomorphs) while the last screen uses a deletion allele of the SNAP-25 gene (null mutation). With these screens we hope to precisely identify the molecular players involved in SNAP-25 mediated endocytosis versus exocytosis.
Feeding Strategies and Prey Preferences in Predacious Leeches
Heather Shumway, Southern Utah University Biology Glossiphoniids are jawless leeches that feed using a proboscis, which they use to impale prey so that they can feed on their prey’s blood and tissues. This study focuses on two different species, Glossiphonia complanata and Helobdella stagnalis. Helobdella stagnalis feed on a variety of prey, including bloodworms and freshwater snails, often feeding together in large groups. When a prey item is placed in the container, H. stagnalis immediately begin smelling the water and moving toward the prey. After the first H. stagnalis makes contact and secures prey, other individuals within the group will move to that area, often passing prey along the way, to attach to the same prey item. Once a number of individuals have started feeding, the prey is often broken into smaller pieces and individuals can then begin feeding on various sized pieces. This is in contrast to members of the species Glossiphonia complanata, who appear to be selective with their prey, and have been observed going up to 4 weeks without feeding, even in the presence of their non-preferred prey (bloodworms). When preferred prey (freshwater snails) were placed in the container, unfed G. complanata did not immediately begin smelling the water or initiate hunting. Unlike H. stagnalis which feed in groups, G. complanata appear to prefer feeding alone, and have even exhibited competitive behaviors.
Adaptation of Staphylococcus Aureus to UV-C Light
Kristian Johnson, Dixie State University Biology Antimicrobial methods, such antibiotics and Ultraviolet (UV) irradiation, have been a means of suppressing prokaryote proliferation for nearly a century. Over the last several years, scientists have found that numerous strains of prokaryotes have developed resistance to antibiotics. Concurrently, the process of bacterial irradiation using UV-C is common practice in a variety of sterilization applications. As revealed in the seminal work by Chang et al. inactivation curves for Microorganisms such as Staphylococcus aureus (Staph) were established in 1985. Their values indicate survival rates based on Intensity, which is defined as the time of UV irradiance per unit area. Similar to the evolutionary evidence of antibiotic resistance, we are interested in the selective pressure UV-C has on Staph. By recapitulating Chang’s experiment nearly 30 years later, our preliminary results indicate an increased resistance to UV-C in Staph. In this experiment, we determine a current UV-C dose-dependent kill rate function for Staph.
Identification of Genes Regulating Sperm Motility in C. elegans
Kandrie MyIroie, University of Utah Human Genetics In C. elegans, sperm activation is the process through which sperm become motile. Genes that regulate sperm motility are being identified through a genetic screen. In this screen, swm-1 mutants that have sperm that activate too early are mutagenized, and genes regulating sperm motility can be found when the sperm of mutant worms no longer activated too early. Two genes have already been identified, try-5 and snf-10, and these can help identify new mutations. By selecting specific strains with sperm activation defects, the mutations causing the defect can be characterized. To sort through the many mutants, complementation tests to identify them as a new gene, or a new allele of try-5, snf-10 are being done. Selectively crossing an unknown mutant strain to a strain with a known mutation in try-5 or snf-10 will help identify the unknown mutation. If the progeny of the cross have activated sperm then the two strains complement each other. In this case the alleles must be in different genes and the unknown mutation from that cross has not been identified. If the progeny instead has non-activated sperm then the mutations failed to complement and the unknown mutation is allelic to the mutation it was crossed with. One new allele of try-5 and of snf-10 has been found. It is suspected that the other strains contain alleles of new genes not previously identified. Further complementation tests will be done to test this theory.
Fire Retardant as an Environmental Risk Factor Contributing to Parkinson’s Disease
Michael Barney, Southern Utah University Biology Parkinson’s disease is caused by a decrease in dopaminergic neurons in the substantia nigra, which results in a loss of motor control. Although the exact causes of Parkinson’s disease are unclear, studies have shown that exposure to environmental contaminants causes death of cells in the substantia nigra (McCormack et al., 2002). The purpose of our study is to investigate the potential role of fire retardant as a risk factor for Parkinson’s disease. We subcutaneously injected 8 experimental mice with retardant solution (1 ml/60 g body weight) and 7 control mice with saline solution (1 ml/60 g body weight). Each mouse was given 8 injections over four weeks, after which the mice were sacrificed and brains were harvested. Frozen sections (40 mm thick) were mounted on slides and are being processed using anti-tyrosine hydroxylase, which will be visualized using a peroxidase reaction. The number of cells in the substantia nigra will be counted and compared between control and experimental groups. Our hypothesis is that exposure to fire retardant is a risk factor for Parkinson’s disease. If this hypothesis is supported, this would be the first study to show a link between fire retardants and Parkinson’s disease. This would have important implications for current forest fire fighting techniques.
Impairment of Withholding a “Pre-Potent” Response In Rats With METH-induced Neurotoxicity
Lee Leavitt, University of Utah Biology Chronic methamphetamine (METH) abuse leads to structural and functional damage in the brain, which likely contributes to cognitive and behavioral dysfunction. Recent data suggest an association between METH abuse and impaired inhibitory control over behavior; that is an impaired ability to inhibit inappropriate actions or thoughts. However, the extent to which METH-induced neurotoxicity is responsible for such impairment remains to be determined. Previously, we reported that rats with METH-induced partial dopamine (DA) and serotonin (5-HT) loss in striatum and prefrontal cortex (PFC) exhibited impaired response inhibition in the form of perseverative responding. Furthermore, levels of serotonin transporter (SERT) binding in PFC correlated with perseverative responding. Herein, we have examined another dimension of response inhibition impulsive action, which is an inability to withhold a “pre-potent” response in rats with METH-induced neurotoxicity. Rats were trained to perform a stop-signal task (SST). Once rats achieved stable responding (>80% correct response) on both “Go” and “Stop” trials, they were treated with saline (0.9%), a neurotoxic regimen of METH (4 x 10 mg/kg, 2-hr intervals, s.c.) under normal ambient temperature conditions (“neurotoxic” METH group) or the METH regimen under cooling conditions (“normothermic” METH group). One week after the treatment, rats were again tested on the SST. After behavioral tests were done, animals were sacrificed and brains removed for determination of monoamine loss. The results showed: 1) the “neurotoxic” METH-, but not the “normothermic” METH or saline-treated rats, showed a 40-60% loss of SERT and dopamine transporter (DAT) binding in PFC and striatum; 2) the “neurotoxic” METH group showed normal behavioral performance in “Go” trials of the SST relative to the “normothermic” METH group or saline controls; 3) the “neurotoxic” METH group exhibited impaired withholding of a “pre-potent” response, as reflected by increased numbers of errors on the “Stop” trials of the SST. Overall, these data suggest that impaired inhibitory control over behavior (i.e., increased impulsive action) can arise as a consequence of METH-induced neurotoxicity to central dopamine and serotonin systems. Supported by NIH grant DA 024036
Experimental Evolution of Feather Louse (Columbicola Columbae) Parasite Body Size
Heidi Montgomery, University of Utah Biology Parasites are arguably the most diverse group of organisms on the planet. One key factor in determining patterns of parasite biodiversity is the number of host species a parasite can infect, or its host specificity. However, surprisingly very little is known about the evolution of host specificity. Here I use domesticated pigeons (Columba livia) and their ectoparasitic feather lice (Columbicola columbae) to demonstrate how parasite body size influences host use. The current study has two main goals: 1) to use different sized hosts to experimentally evolve louse body size, and 2) to determine how parasite body size effects host specificity. Four breeds of domesticated pigeons were infested with parasitic feather lice. After roughly six generations, mean louse body size changed with respect to host body size, with the largest pigeons having the largest lice. Interestingly, the smallest pigeons had larger lice as well. However, this increase in louse size appears to be the result of a change in microhabitat use, with lice shifting from predominantly the wings and tail to the head. This study is one of the first to experimentally demonstrate how changes in parasite morphology and behavior influence the evolution of host specificity.
“Killing Me Softly”: The Economic Toll of Forest Fire Repression
Adam Olson, Brigham Young University Plant and Wildlife Sciences Forests systems of the Central Rocky Mountains rely on an intricate balance of natural disturbance cycles in order to develop properly. Forest fires are one such disturbance, however, certain fire characteristics, particularly fire severity, can vary widely across forest landscapes. In our study, we examined the influence of fire severity on aspen regeneration as well as aspen defense against wildlife and livestock browsing. Our results indicate that high or moderate burn severity is more favorable to aspen regeneration and survival. These areas of severe burn result in a higher density of aspen suckers, more vertical and lateral growth, greater chemical defense concentrations, and less browse damage than the neighboring plots of low or no burn. This data suggests that fire severity should be taken into account when considering plant regeneration and susceptibility to browse damage in burned landscapes.
Sirt1-Mediated Suprression of Cell Death in Breast Cancer
Matthew Whited, Brigham Young University Biochemistry Several lines of evidence suggest that protein lysine acetylation pathways are deregulated in cancer (1). Moreover, deacetylase inhibitors are emerging as important anti-tumor therapeutics, suggesting that the forced reprogramming of protein-lysine acetylation is toxic to tumor cells. In this study we show that Sirt1, an NAD+-dependent Sirtuin deacetylase that promotes cancer cell survival, is aberrantly mislocalized to the cytoplasm of breast tumor cells. Moreover, the depletion of cytosolic Sirt1 by siRNA sensitizes breast tumor cells to paclitaxel-induced death. Previously, we developed a biotin-switch proteomics approach to identify cytosolic Sirt1 substrates (2). This approach yielded a variety of substrates with roles in metabolism, survival, and oxidative stress signaling. Our current work focuses on three of the proteins identified as Sirt1 substrates: SOD1, DJ-1, and 14-3-3z. SOD1 and DJ-1 both suppress oxidative stress-induced death, and high levels of 14-3-3z expression suppress chemotherapy-induced apoptosis and correlate with negative patient outcomes in breast cancer. Our preliminary results suggest that acetylation of DJ-1 and SOD1 suppress their anti-oxidant functions, while acetylation of 14-3-3z disrupts its binding to pro-survival proteins. Taken together, our data support a model in which cytosolic Sirt1 activates multiple pathways that work together to promote tumor cell survival.
Tissue Specific Isolation of Nucleosomes in Caenorhabditis elegans
Hyrum Shumway, Brigham Young University Microbiology and Molecular Biology Epigenetics is the study of factors of gene regulation that do not stem from the primary DNA sequence. One such example of epigenetic gene regulation is where DNA wraps around histone proteins to form nucleosomes. The positioning of nucleosomes is the first order of control for genic transcription. Wrapped DNA is less accessible for transcription compared to DNA that is nucleosome free. Model organisms for human epigenetics such as the nematode Caenorhabditis elegans are invaluable because of their ease in manipulation and because the components of nucleosomes (histone proteins and DNA) are highly conserved across phylogeny. The purpose of my particular research is to develop and validate a new protocol for tissue-specific isolation of nucleosomes through immunoprecipitation in young adult C. elegans. This protocol leverages green fluorescent protein fused to histones to research nucleosome positioning in the germ-line cells of C. elegans nematodes. When successful in our animal model, this procedure is the first step in allowing analysis of chromatin architecture of any tissue at any developmental or disease state including human cells. The widespread prevalence and implications of human disease is staggering in magnitude. Research is ongoing to decrease morbidity, increase prevention, and fight infection. With epigenetic tools, this vital research is benefitted and supplemented.
The Effect of Male Dominance on Female Mate Choice in Poecilia gillii
Michael McEntire, Brigham Young University Biology Female mate choice (intersexual selection) and male dominance interactions (intrasexual selection) can each play important roles in sexual selection. These two mechanisms tend to be discussed in isolation. The goal of this study is to explore the interaction between these two forms of sexual selection. To test this idea, we focused on the livebearing tropical fish system Poecilia gillii. We grouped males into similarly sized pairs and observed them for a week to determine which male was dominant. These pairs were then presented to females in mate choice trials to ascertain female preference. We also photo- graphed the males to determine coloration. We found that females were unable to detect dominant males without viewing the physical contest and that carotenoid coloration bore no effect on female preference. Females tended to choose the male to their left, suggesting the preferential use of their right eyes in making decisions on mate choice.
The Role of Timing of Dietary Selenium and Isoflavone Supplementation in the Reduction of Prostate Cancer Risk Factors in TRAMP Mice
Ji Su Park, Brigham Young University Nutrition, Dietetics, and Food Science Selenium (Se) and soy have each been shown to reduce risk for prostate cancer when consumed at high levels. The purpose of this project was to define the molecular mechanisms of prostate cancer chemoprevention by Se and soy, and to describe how timing of dietary treatment modifies those effects. [C57BL/6 X FVB] F1 TRAMP (TRansgenic Adenocarcinoma of Mouse Prostate) male mice were fed stock diets high or low in soy, with or without a supplement of Se (4.0 mg Se/kg BW as Se-meth- ylselenocysteine) by gavage 5 d/wk in a 2 X 2 factorial design. Mice were exposed to different diets starting from conception, 6 weeks, or 12 weeks of age and were sacrificed at 18 weeks. Three-way ANOVA showed that supplemental Se increased serum and liver Se, with significant interactions with both time and soy intake. Selenium dosing decreased BW independent of soy intake and time of dietary intervention. Both Se and soy decreased epididymal fat pad weights, with Se’s effects being more pronounced in mice exposed to diets from conception than from 6 wk. Urogenital tract weights, a measure of prostate proliferation and tumor volume, were significantly reduced by Se supplementation (P<0.001) and soy (p=0.044), independent of time of dietary intervention. Histological examination of mouse prostates is in progress to determine dietary effects on disease progression. These data suggest that, in this model, chemopreventive efficacy of Se and soy does not differ between prenatal and early post-natal introduction.
The Effects of DNA Methylation on Nucleosome Positioning
Marcus Vranes, Brigham Young University Molecular Biology Recent studies have attempted to discover the correlation that exists between DNA methylation and nucleosome positioning, but none have explored the direct effect of DNA methylation on nucleosome formation and positioning. This proposed research will directly test the effects DNA methylation has on nucleosome positioning and whether the histone octamer has preferred sequences to which it binds, which will in turn add our understanding of gene expression and regulation. A better understanding of these concepts will help to aid efforts in gene therapy to better the quality of life of many who suffer from various genetic conditions.
Characterizing the Role of HspB2 in Cardiac Metabolism and Muscle Structure Using Yeast and Mammalian Cells
Whitney Hoopes, Brigham Young University Microbiology and Molecular Biology HspB2 is one of eleven known small Heat Shock Proteins (sHSP) that is expressed in human heart and skeletal muscle. In response to cellular stress, heat shock proteins play a vital role to help misfolded proteins and proteins susceptible to denaturation maintain their structure. Two members of the sHSP family, CryAB and HspB2, are both required for normal heart function and cardiac muscle integrity. CryAB-deficient mice have defects in cardiac muscle structure whereas HspB2-deficient mice display energy deficits (Rajasekaran et al. 2007). The contrasting phenotypes of CryAB and HspB2 suggest differential roles for these molecular chaperones in the heart. HspB2 has been found to localize with the mitochondria in several different cell lines and overexpression of this sHSP has been shown to support survival of cells against heat stress (Nakagawa, 2001). To understand the role and mechanism of HspB2 in cardiac muscle energy regulation, we have used a yeast two-hybrid (Y2H) system to uncover the novel protein binding partners specific to HspB2. From screening a human heart cDNA library, HspB2 interacted with approximately 10,000 out of 20 million plasmids. We have sequenced more than 1000 of these putative interactors and have identified over 100 unique proteins. Over 40% of these protein partners are involved in mitochondrial energy production and another 25% in cardiac muscle structure maintenance. In addition, we have identified an interaction between HspB2 and the related sHSP CryAB. We then compared this data with mitochondrial HspB2 binding partners identified by mass spectroscopy (MS) through a large-scale bioinformatics analysis and constructed a protein-protein network. Y2H dependency tests were conducted to verify interactions identified by both Y2H and MS. Following yeast verification, a subset of the interactions were confirmed in C9H2 cardiac cells through coimmunopurification. Our research describes the first protein-protein interaction network for any sHSP, supports a role for HspB2 in mitochondrial energy production and suggests a link between mitochondrial energy production/redox stasis and stressed cardiac muscle maintenance.
Early Parental Death, Genetic Variants and Risk for Alzheimer’s Disease: Building a Risk Profile from the Cache County Study on Memory, Health, and Aging
Michael Peterson, Brigham Young University Biology A person’s predisposition to Alzheimer’s Disease is known to be influenced by both genetic factors as well as environmental factors. One know environmental factor is that known to affect risk for disease is early parental death. The purpose of this research is to better understand the complex factors that influence the disease by analyzing the relationship between the environmental factor of early parental death with genetic variants known to influence the disease. We used extant data from the CCSMHA, an ongoing aging study including 89.7% (5092 of 5677) of all of the eligible residents of Cache County, Utah. This data includes information about environmental and psychosocial stressor of the subjects as well as information about physical examinations, metal screenings, and individuals’ genotypes at many loci that are known to be related to Alzheimers Disease. We used multivariate logistic regression to determine the effect of early parental death by SNP interactions on risk for AD. For the analysis we cleaned the data by removing SNPs less than a minor allele frequency of 0.01, a Hardy-Weinberg equilibrium value of 110-6, and a maximum missing snp call of 0.2. Individuals were also removed if genotyping rate was less than 0.2. After filtering we had 262 cases, 239 controls and 0 missing Final Results will be presented at the Conference.
Genome-Wide Association Study of Visinin-Like Protein Levels, an Endophenotype for Alzheimer’s Disease
Rachel Perry, Brigham Young University Life Sciences Previous studies have indicated that Visinin-like protein (VILIP) may be a powerful tool in predicting disease progression and guiding prognosis of Alzheimer’s disease (AD). Cerebral spinal fluid (CSF) was collected from hundreds of individuals with varying levels of AD. The CSF was then analyzed for levels of VILIP protein using Luminex technology. SNPs were genotyped using the Illumina OmniExpress chip. SNPs found to have a Hardy-Weinberg frequency less than 1×10-4 were not included, assuming that this variance was due to a genotyping error. SNPs and samples missing more than five percent of the data were also not included. Following the cleanup of the data, an association test using linear regression was performed. Covariates used in the analysis included age, gender, and covariates that accounted for population stratification (PC1 and PC2). Over one hundred SNPs were found with a p-value less than 1×10-5. The genomic inflation factor for the generated data was 1. One marker showed significance at the genome-wide level. We have identified a genetic marker that shows significant association with CSF VILIP levels. This finding may provide insight into genetic control of VILIP levels, which may be a useful in understanding the pathological processes involved in AD.
The Association Between Pattern Separation and Depression
Matthew Schneider, Brigham Young University Physiology and Developmental Biology Alzheimer’s disease, the most common form of dementia, affects millions of people per year. Research has shown that Alzheimer’s affects the hippocampus brain region, which is involved in learning and memory. Understanding learning and memory functions is imperative to comprehending both healthy brain functions and Alzheimer’s disease. Many researchers seek to understand both the causes and treatments of the disease, but tangible information remains elusive. Studies thus far have shown that to encode memories, the brain must change neural synapses to either strengthen or weaken those pathways, a process known as synaptic plasticity. Using electrophysiology techniques on mouse hippocampal slices, this project will provide further insight on memory formation and regulation by imitating synaptic plasticity mechanisms. I will look at a specific cellular pathway involving the protein receptor GPR55, which has recently been shown to induce synaptic plasticity. By understanding how the GPR55 pathway functions, this research will contribute to the understanding and treatments of Alzheimer’s and other neurodegenerative diseases.
The Effects of Exercise on Synaptic Plasticity in the CA1 Region of the Hippocampus in Mice Who Experience Acute Stress
David Marriott, Brigham Young University Physiology and Developmental Biology Acute stress has been shown to decrease Long-Term Potentiation (LTP) in the CA1 region of the mouse hippocampus. Additionally, stressed animals show signs of anxiety and suffer decreases in spatial memory tasks such as object recognition and maze navigation. Conversely, exercise has been shown to increase spatial memory task performance in mice, attenuate anxiety-like behaviors and enhance neurogenesis and LTP in the dentate gyrus. While the effects of stress and exercise have been examined independently, there is currently a lack of experimental evidence that connects how stress and exercise, when experienced by the same animal, might modulate LTP in the CA1 region of the hippocampus. In our ongoing study, mice have been separated into a control group, a stress group (restraint and tail-shock), and an exercise + stress group where mice have voluntary access to a running wheel (for 30 days) before undergoing the stress protocol. We hypothesize that exercised animals will experience a protective effect against the reductions in CA1 LTP. In the stress only group, preliminary data shows a modest stress effect on LTP, yet we are learning that factors such as controllability of the stressor or the ability to develop coping mechanisms might potentially attenuate
Modified Nucleosomes and the Effect on Positioning
Tara Hammond, Brigham Young University Microbiology and Molecular Biology Genetic diseases, including Alzheimer’s, cystic fibrosis, and many cancers, can be detrimental to individuals and their families. Gene therapy can possibly cure these diseases by inserting a correct copy of the gene into the chromosome, upregulating good genes, or downregulating the harmful gene. When DNA is packaged into a cell, it wraps around histones-an octamer made up of two tetramers, each containing four different subunits to create nucleosomes. Where the nucleosome sits on the DNA sequence determines whether or not a gene can be transcribed. In heterochromatin, nucleosomes are denser and DNA is tightly packed, thus causing genes to not be transcribed. Euchromatin contains looser packed nucleosomes and therefore has higher transcription levels. This project seeks to determine if modified nucleosomes have DNA sequence preferences. We are working with histone H3 to tri-methylate lysine 4, which has been shown to correlate with euchromatin. The modified histone will be used to create octamers. C. elegans DNA will be added to modified histones and to unmodified histones and allowed to create nucleosomes. The wrapped DNA will be sequenced, allowing us to compare the modified and unmodified nucleosome DNA preference. The difference in preference will enhance our ability to know how to move nucleosomes, thus aiding in gene therapy.
APOE e4 Independent Associations in the APOE Gene Region with Cerebrospinal Fluid Levels of Amyloid Beta 42 in Alzheimer’s Disease
Spencer Foutz, Brigham Young University Biology CSF AB42 levels are a biomarker for Alzheimer’s Disease. The APOE e4 allele associates with CSF AB42. Little is known about SNPs in the region independent of apoe e2/e3/e4 isoforms. By adjusting for the effect of these isoforms, statistical analysis uncovered new SNPS associated with CSF AB42. Information was used from 1338 individuals from four datasets, specifically: The WU-ADRC, ADNI, University of Washington, and UPENN. Samples included individuals with and without AD. The 169 SNPs used were extracted from the APOE region and surrounding 50 kb using 1000 Genome Software. Linear regression analysis was performed, adjusting for specific covariates. Adjustments were made for the APOE e2 and e4 alleles before repeating the analysis. Significant SNPs were tested in e3 homozygous individuals. Each series was separately analyzed and combined in a meta-analysis for confirmation. P-values, sample sizes, and effect sizes were used in the meta-analysis. Results from these analyses allowed us to conclude rs769449 is associated with lower levels of CSF AB42 and acts independent of the APOE e4 allele.
Chytridiomycosis-resistant Frog Populations in Southern Utah
Chancen Hall and Nichkolas Hadley, Dixie State University Biological Sciences Batrachochytrium dendrobatidis (chytrid fungus) is prevalent worldwide, and the resulting chytridiomycosis has contributed to at least 168 amphibian species extinctions. In 2010, B. dendrobatidis was discovered in the greater Zion National Park area of southwestern Utah. Because few populations have shown resistance to chytridiomycosis, we decided to explore the effects of this disease on populations of Hyla arenicolor (canyon tree frog). We tracked the spread of B. dendrobatidis by testing skin samples taken annually from several different canyons and monitored population sizes. During the three years of our study, infected populations did not show subsequent population declines. This suggests that H. arenicolor population size in this region is unaffected by B. dendrobatidis. In the future, testing hypothesized explanations for surviving infection could help us identify populations not at risk and thus allocate conservation resources more efficiently.
GPR55: A Potential Enhancer of Learning and Memory in the Hippocampus
Rachel Schneider, Brigham Young University Neuroscience The ability to create distinct memories for very similar stimuli and events is called pattern separation. Pattern separation is thought to be dependent on neurogenesis (the birth of new neurons) in the dentate gyrus, a subregion of the hippocampus. Neurogenesis is reduced in depression, as is overall memory performance. It has been proposed that depression negatively impacts pattern separation abilities, however a link between depression and performance in pattern separation memory tasks has yet to be identified. Accordingly, we designed a study to investigate the relationship between pattern separation performance and level of depression. Eighty-two participants completed a pattern separation memory test and a set of questionnaires to gauge their level of depression. During the task, participants were presented with 600 images one at a time on a computer screen in a continuous recognition paradigm. Participants were asked to determine whether each image was new, old, or similar. Images seen for the first time during the task qualified as “new”, images that were repeated following a variable delay qualified as “old”, and images that were similar to previously presented stimuli, but not exactly the same, qualified as “similar”. A pattern separation score was calculated based on the proportion of correctly identified similar stimuli. We found a negative correlation between depression scores and pattern separation scores (r(82) = – 0.301, p < 0.01). This relationship held constant even when we controlled for other factors known to affect neurogenesis, such as exercise and anxiety levels. These results provide support for the theory that depression is negatively related to pattern separation performance, possibly due to a decrease in neurogenesis in the hippocampus.
Endocannabinoid Biosynthesizing Enzyme Expression in Hippocampal Stratum Oriens Neurons
Ryan Williamson, Brigham Young University Physiology and Developmental Biology The hippocampus is thought to mediate learning and memory by altering the strength of synapses within its circuitry. In many cases, this synaptic plasticity can be induced by intracellular signaling molecules. Lipid-based intracellular signaling molecules called endocannabinoids have been shown to modulate or mediate synaptic plasticity among hippocampal pyramidal cells and stratum radiatum interneurons; however, the role of endocannabinoids in mediating synaptic plasticity among interneurons in the stratum oriens is still unclear. Our goal was to determine whether stratum oriens interneurons have the machinery necessary for endocannabinoid production and, if so, whether this machinery is expressed in a sub-type specific manner. To do this, we used patch clamp electrodes to extract single cells from rat hippocampal slices and analyzed the expression of endocannabinoid biosynthetic enzyme mRNA using quantitative real-time PCR. In this analysis, we examined cellular expression of two interneuron markers, GAD65 and GAD67, as well as several calcium-binding proteins and neuropeptides to determine interneuron subtype. We also analyzed cellular expression of several endocannabinoid biosynthetic enzymes, including N-acyl phosphatidylethanolamine phospholipase D, diacylglycerol lipase alpha, and 12-lipoxygenase, as well as type I metabotropic glutamate receptors. Preliminary data suggests that stratum oriens interneurons express mRNA necessary for endocannabinoid biosynthetic enzymes. Additionally, we identified interneurons that coexpress mRNA for somatostatin and diacylglycerol lipase, suggesting that O-LM cells or another somatostatin-positive interneuron subtype may possess the enzymes necessary to produce the endocannabinoid 2-arachidonoylglycerol. Further work will allow us to examine how endocannabinoid biosynthetic enzyme expression correlates with other interneuron subtypes in the stratum oriens.
Validation of Cache County Genotype Data
Aaron Sharp, Brigham Young University Biology The Cache County study on memory, health, and aging has played a significant role in several studies. However, there is some potential skepticism in the scientific community about its sample. The population in Cache County is derived from a diverse group of founders, but it is perceived by some to be an isolated population. If so, conclusions discovered there might not apply to other populations. Our objective is to compare the Cache County data to a panel of genetic data—provided by the International HapMap Project and the Alzheimer’s Disease Neuroimaging initiative—that is known to be representative of typical European-American populations. Doing so will indicate whether the genetic diversity in the Cache County sample is characteristic of an isolate or not. Analysis will be done using the open source “Plink” analysis toolset, including the –cluster and –mds-plot computational algorithms. Using –cluster groups individuals according to identity by state distances. The –mds-plot algorithm creates a scatter-plot of the individuals in 2-dimensional space, identifying any systematic difference between the Cache County data and the general population. We expect that the Cache County data will be representative of general European-American populations, because of its diverse group of founders.
The Effects of Temperature and Water Availability on the Germination of Bromus Rubens
Rachel Nettles, Brigham Young University Plant and Wildlife Sciences Background/Questions/Methods
Association of the CETP Gene with Cognitive Decline and Dementia in the Cache County Study
Caitlin Munger, Brigham Young University Biology Alzheimer’s is a fatal, non-treatable neurodegenerative disease and the most common cause of dementia. While no one gene has been found to determine the development of Alzheimer’s, past studies have established a strong hereditary influence on Alzheimer’s. So far, only 5 genes have been found which replicably contribute to the genetic risk of developing Alzheimer’s. However, the gene for Chlolesteryl Ester Transfer Protein (CETP) has been identified as a possible new contributor to the genetic risk factor. In order to test this association we obtained data on over 4000 subjects studied in the Cache County Study on Memory, Health and Aging over a 15-year period. This data included DNA samples, cognitive decline rates and incidence of dementia–particularly Alzheimer’s Disease. DNA samples were SNP genotyped using quantitative PCR. The SNP genotypes and corresponding phenotypes for each subject were then analyzed for association usingmixed linear models and for survival, or the amount of time until the disease appeared, using Cox proportional hazard models. We found a correlation between the V405I SNP and a decreased rate of cognitive decline. We found that for each additional G the rate of decline decreased by 0.6 points per year on the MMSE test. The identification of CETP as a player in the genetic risk for Alzheimer’s and dementia will provide much needed information on the genetic factors involved in dementia and allow for possible future therapeutic targets.
Subduing the Flu: New Alternatives to Amantadine
Joseph Moulton, Brigham Young University Physiology and Developmental Biology With the advent of recent mutations in the influenza A viral genome, drugs that previously blocked the proton flux responsible for disassembly of the viral envelope and exposure of viral RNA to the transcriptional machinery of the host cell have become ineffective. Our study of the M2 hydrogen ion channel responsible for this flux has led to a vastly-increased under- standing of the mechanisms behind the conductance activity and potential blockage of these transmembrane tetramers. By embedding M2 proton channel subunits of the S31N mutant strain into liposomal bilayers and suspending these bilayers in the buffers and ionic gradients characteristic of the intracellular environment, we have been able to simulate and observe nor- mal functioning of the influenza A virus. Using these liposomal bilayers, we have developed a series of experimental protocols to test a variety of amantadine- and rimantadine-related drugs for successful blockage of M2 S31N proton conductance. Our research presentation will be centered around the mechanisms of this channel and the favorable results that we have obtained from many of these drugs.
Guerrilla Warfare Theory and Praxis: The Case of Revolutionary Leader Ernesto ‘Che’ Cuevara
Anna Maria Guadarrama, Utah State University Languages, Philosophy, and Communication Studies Guerrilla warfare is an ancient style of armed conflict that continues to be practiced throughout the world today. Many countries and peoples have either taken part in guerrilla warfare or have sought to counteract irregular war with counterinsurgency methods (e.g. United States). This thesis project consists of an analysis of the guerrilla warfare theories and battlefield strategies utilized by Ernesto ‘Che’ Guevara—the Argentine-born Cuban revolutionary considered by most people as the premier thinker on the nature of guerrilla warfare. Guevara developed his foco theory of guerrilla warfare based on the model of warfare used successfully during the Cuban Revolution, and then sought to replicate this achievement in The Congo and Bolivia. After a brief introduction on the nature of guerrilla warfare in general, the thesis proceeds to analyze the theoretical writings on guerrilla warfare by Ernesto ‘Che’ Guevara, as seen in his seminal treatise Guerrilla Warfare: A Method (1963). The thesis then proceeds to analyze Guevara’s application of his foco theory as an on-the-ground guerrilla leader to evaluate the praxis of Guevara’s foco theory during his guerrilla campaigns in Cuba, The Congo, and Bolivia.
Cold War to Holy War: The Soviet-Afghan War and Jihad
Nina Cook, Utah Valley University History As events of the 1978 April Revolution in Afghanistan played out during the Cold War, U.S. policy makers became concerned about the Soviet sphere of influence and began to fund the Mujahedeen-rebel groups that formed in Pakistan. The Mujahedeen, inspired by jihad, remained divided across ethnic lines, began a religiously inspired struggle against Communist usurpers and oppressors. The United States saw the Mujahedeen as a useful Cold War tool in order to contain Soviet expansion and therefore throughout the 1980’s the United States continued to head the effort to supply the rebels with money and weapons. This aid was crucial in the Soviet decision to withdraw from Afghanistan and contributed to the eventual breakup of the Soviet Union. Yet, many of the Arab Mujahedeen saw this outside the Cold War context, as a victory for concepts of militant Jihad. Thus, the Soviet-Afghan war became a catalyst for the ideas of Radical Jihad, which would lead to a global holy war against the U.S and the West by the al Qaeda network, created during the Soviet-Afghan war, beginning in the 1990s. The significance of the Soviet-Afghan War, then, lies in some unintended consequences for the U.S.: Cold War containment of the Soviets in Afghanistan fueled Jihad, which in turn targeted American interests in an entirely new war.
Women in Utah, Shattering Patriarchy during Second Wave Feminism
Kimberly Williamson, Utah Valley University History “There is nothing particularly interesting about one’s life story,” Eleanor Roosevelt wrote, “unless people can say as they read it, Why, this is like what I have been through. Perhaps, after all, there is a way to work it out.” Humans throughout time have recognized the need for storytelling and have been preserving oral histories. Narratives supplement our historical memory and offer an in-depth account of personal experience and reflections, which allows another to feel a commonality that often dissolves the barriers of race, class, gender, and even time. During the 19th century, the fight for enfranchisement united Utah’s early settlers with national suffragists. Feminists such as newspaper editor, Emmiline B. Wells and “presidentes” of the women’s organization within the Church of Jesus Christ of Latter-day Saints (LDS), Eliza R. Snow, were in the forefront of this movement. Wells, Snow, along with other women were actively involved in their family responsibilities. However, they also held public and political positions within their communities that were not typical for women of that period. The women’s movement of the seventies recognized that literature wasn’t acknowledging women’s prominent role in society. Not only was there a lack of sources by and about women, but the historiography in general was male dominated. Hence, the LDS church initiated a crusade to collect women’s journals, letters, and other writings of Utah’s pioneers. These sources increased scholarship of Utah’s suffragists, which caused national recognition of the role they played during First Wave Feminism. Nevertheless, there is a trivial amount written about the women in Utah during Second Wave Feminism from the 1970’s to late 1980’s. My research focuses on stories of women in Utah during Second Wave Feminism. I interviewed four women within higher education where they expressed personal experiences that are similar in spirit to Utah’s early settlers. Inadvertently each woman had some connection with the LDS church. My thesis will argue that by extrapolation there were many women, particularly at Utah Valley University who transcended patriarchy to achieve positions of leadership and notoriety. Their personal narratives challenge the feminist theory of patriarchal suppression, which seems paradoxical considering the fact that Utah’s dominant religion, the LDS church, functions as a male governed society.
Creating a Sense of Home: Examining the Personal Belongings of World War II Servicemen
Paul Greenhalgh, Weber State University History Much has been written about the tactics and strategies of World War II, as well as the effects of the war on the world. Far less, however, has been written about the cultural aspect of the war experience, and in particular about the day-to-day culture of the servicemen during the war. Archival research at the Library of Congress and The Institute on WWII and the Human Experience at Florida State University yielded the bulk of the primary sources while a review of the literature on WWII and the culture of U.S. servicemen during the war provided context. Additionally, a cross-disciplinary review of psychological literature on pets and their importance to well-being, as well as why it is important for people to create a sense of home, has nuanced the research. This study looks at the personal effects that servicemen had during the war and how their possessions contributed to the creation of a sense of home while they were deployed. Servicemen carried a vast variety of possessions with them that were not Government Issue. These possessions included diaries, pictures of, and letters from, loved ones, Bibles, pets, musical instruments, and talismans. Moreover, in letters and in diaries, these servicemen have described why they personally have carried these objects with them. This study adds to the body of knowledge on the cultural aspect of WWII servicemen, and therefore adds to the overall understanding of WWII and the culture of war in general.
Finding Social Values from Social Outcasts
S. Geneva Balin, Weber State University Anthropology In the past, mythology served as a guide to how life should be lived and provided a context for the condition in which people found themselves. As the sacred gave way to the secular, mythology lost its power to influence and guide the people as it did in the past. Rather than myths, people turned to literature for guidance. These stories have been told and retold through different eras in history. They have also been shared in contemporary forms such as books and movies which now reach a large audience. This paper will examine stories of social outcasts in an interpretive exploration into culture through the lens of literature. The multiple versions “The Phantom of the Opera” and “The Hunchback of Notre Dame” reflect the cultures in which they were produced as well as the contemporary cultures that love them. A basis for comparison will be established by first recounting, in summary, the plotline for each of the novels. Then theories relevant to exploring meaning will be approached, notably those of Claude Levi-Strauss, Émile Durkheim, Dame Mary Douglas and Carl Jung. From here, variations of the stories and the history surrounding them can be examined to discover potential social meaning and show the importance of stories as a way to bind a culture together.
The “Slave Morality” of the Working Class Promise and the “Domination” of the American Dream
Betty Stoneman, Utah Valley University Philosophy The “American Dream” and the “Working Class Promise” are ubiquitous ideologies in American culture. For this paper, I will argue these ideologies are social constructs which perpetuate and reinforce discrimination, the social hierarchy and the domination of the American working class. First, I will define the key concepts of this paper: ideology, stereotyping, domination, discrimination, the ideologies of the American Dream and the Working Class Promise. Secondly, I will argue these ideologies, as defined by communications professor Kristen Lucas, lead to discrimination and domination of the working class. I will argue the Working Class Promise is an example of philosopher Frederick Nietzsche’s slave morality, where a lower status group attaches positive values to themselves which only serves to perpetuate discrimination against them. Further, I will argue domination arises from such discrimination by examining a study by psychologists Shannon K. McCoy and Brenda Major regarding positive stereotyping, self-stereotyping, discrimination and domination. Next, I will argue, using sociologist Pierre Bourdieu’s concept of habitus, both the Working Class Promise and the American Dream are structured and structuring social concepts which reinforce discrimination and domination through exclusion. In line with Bourdieu, I will use social philosopher Louis Althusser’s arguments to show how the Working Class Promise and the American Dream reproduce the roles of the social hierarchy and domination. I will support this demonstration with evidence from McCoy and Major’s study showing how various forms of discrimination are reproduced in society when individuals believe in the American Dream. Having accepted the social insights of these scholars, I would propose, based on the arguments of philosopher Immanuel Kant regarding the intrinsic worth of rational beings and contrary to elitist or socialist views, the solution is for Americans to reject these ideologies.
Forte! Forte! Sound the Syllogism! Question the Composition! The Relation of Reason and Music in Nietzsche
Duke Cruz, Westminster College Philosophy This research focuses on the nineteenth-century philosopher Friedrich Nietzsche, his philosophy, and how that relates specifically to what he thinks about music. In general this presentation will consist of three main parts: 1) analyzing passages on music in a few of Nietzsche’s works, specifically, “The Birth of Tragedy”, “Beyond Good and Evil”, and “Twilight of the Idols’” with supplementary material relating to his philosophy taken from “The Anti-Christ”, and Walter Kaufmann’s biography of Nietzsche “Nietzsche: Philosopher, Psychologist, Antichrist”; 2) showing the relation of music and reason in each example; 3) postulating the importance of this relation. Throughout my research on Nietzsche and his thoughts surrounding music, it is apparent that whenever he discusses music, it is never about the specific score, keys, melodic content, etc. Instead, I found that in most of the passages that I have ran across, Nietzsche’s thoughts on music collide directly and importantly to his philosophy. In this presentation I aim to elucidate what the importance of this relation between music and reason could be for Nietzsche.
Migrant Head Start in Brigham City, Utah
Carlos Junior Guadarrama, Utah State University English My poster explores the history of the Migrant Head Start program at the former Indian Intermountain School location in Brigham City, Utah. No one to my knowledge has gathered a history of this program, which operated from the mid-1980s until the early 2000s. I intend to explore this Migrant Head Start’s foundations as an informal school for the children of Latino migrants, as well as how it grew and developed over the almost two decades that it existed, before it became the Centro de la Familia de Utah. I plan to interview a former principal, several teachers, as well as former students. I argue that this Head Start played an extremely important educational and social role in the lives of many inhabitants of Brigham City.
Look Who’s Talking: Exploring Writing Conference Interactions and Subsequent Revision
Ryan Krage, Utah Valley University English and Literature Within Freshman Composition Studies, little research has been produced on writing conferences between students and teachers since Laurel Johnson Black’s Between Talk and Teaching: Reconsidering the Writing Conference in 1998. Most published research has not included any systematic information about the nature of the writing conference, a consultation between the student and teacher designed to discuss the student’s paper in order to make changes. Student feedback and involvement in this study is of crucial importance because there is no existing data whether teacher-student conferences are working from a student perspective. The major research question is “What is the relationship between types of teacher-student conference interaction and subsequent revision of students’ papers?” The purpose of the research is to discover what the writing conference between teacher and student accomplishes in terms of better writing outcomes, what the expectations of the student are, and what and how the power dynamics between student and teacher affect the quality and quantity of revision from both student and instructor perspectives. We will examine the connection of students’ perceptions about the writing conference to their final scores and other data. During the spring 2012 semester, we drafted three surveys designed to capture students’ perspectives and expectations regarding the conferences (a pre-conference, post conference, and post final grade). The methodology includes transcribing voice recordings of conferences, collecting student surveys before and after conferences, and collecting students’ rough and final drafts for two papers during the semester. This raw data will be coded into usable data, which will then be analyzed to identify behaviors that facilitate or impede the conference and determine its overall effectiveness. We hypothesize that a dialogic conference will occur when the power dynamic between student and teacher is more equitable, resulting in more student-ownership over the revision process. The extent of student ownership within the revision process will be determined by both the quantity and quality of revision. These findings will, in turn, enable teachers to take a closer look at the nature of their own writing conferences in order to develop better consultations with their students.
The Novel of Chivalry: A Definition of Genre from the Point of View of its Most Avid Reader,don Quixote of the Mancha
Charles Ankenman, Brigham Young University Spanish and Portuguese The Middle Ages and the cultural, political and social coming of age of the European continent produced a corpus of literature and a literary tradition representative of its religious and cultural values that, amazingly, still continues to influence society today. Commonly employed terms such as “a knight in shining armor”, “prince charming”, and parallels occasionally drawn between children and both literary as well historical princes and princesses of yore are evidence of the longevity of the influence of Medieval culture, it’s ideals and the literary genre that most perfectly reflected it: the novel of chivalry. Today, few academics study the genre, it is seldom analyzed in universities, and almost never read outside of academia. In fine, their legacy and their memory has largely been preserved in the endearing pages of Cervantes’ masterpiece, Don Quixote. In this work, the protagonist, don Quixote, goes mad as he credulously devours his extensive private collection of chivalric literature believing all he reads to be not only true but also worthy of imitation. Nevertheless, despite Don Quixote’s wide reading public, few have ever read a novel of chivalry and, as a result, the exact definition of the novel of chivalry can be both obscure and even beyond the grasp of the modern reader who is uninitiated in the field of Medieval and Renaissance chivalric literature. Nevertheless, through a careful analysis of don Quixote’s private collection of novels of chivalry, the mega-genre that they constitute and the sub-genres that comprise it; the chivalric genre can be clearly defined. Additionally, through the comparative analysis of the English term novel of chivalry as opposed to the Spanish term libros de caballerias (book of great deeds done on horseback) the importance of language’s influence on the perception of the world will be demonstrated and Jacques Lacan’s theory regarding language and perception substantiated. Finally, this analysis will elucidate the novel of chivalry’s status both as a continuation of the great classical epic tradition as well as another link in the universal tradition of epic poetry and storytelling. This presentation will be of interest both to Medievalist as well as specialists in the Renaissance, Don Quixote, Spain and Portugal. Power Point Presentation. 20 – 30 minutes.
Bela Kondor & the Transatlanticism of the Faux-Naive Tradition
Kev Nemelka, Brigham Young University Art History and Curatorial Studies Hungarian art has yet to break across the Atlantic with much influence on the art world, but perhaps the oeuvre of Béla Kondor (1931-1972), considered by many Hungarian art historians to be the starting point of Hungarian contemporary art, may have a shot at drawing the nation’s art out of obscurity and into the light of American contemporary “faux-naïve,” an art tradition with a growing momentum that could provide fertile terrain for Kondor’s comparable aesthetic. The faux-naïve tradition springs from “naïve art,” an art tradition whose name itself has been somewhat controversial in the past decade but has nevertheless influenced artists all over the globe. Particularly in Utah Valley, a number of artists and art professors—e.g. Andrew Ballstaedt, Fidalis Buehler, and Brian Krishisnik—are developing their own faux-naïve versions of contemporary folk art similar to that of Kondor with no knowledge of his existence, and although some contend that the sincerity of faux-naïve is factitious and premeditated, the secular and religious works of Kondor and these American artists show the positive side of contrivance, that faux-naïve can provoke feelings of nostalgia and insight into real emotions, focusing our attention on adolescent memories or spiritual innocence alluded to in the works rather than on the lack of complexity, precision, or realism often sought after by aficionados of conventional, believable art. I personally interviewed numerous Kondor experts and curators of Hungarian museums, recovered archived articles on the artist held in Hungarian collections, and conducted comparative analyses of Kondor and the aforementioned Utah artists, whom I also interviewed regarding the subject of faux-naïveté.
Looking Beyond the Wall: A Philosophical Look at Themes of Madness, Chaos, Loneliness, and Suicide in Fin-de-Siecle German Women’s Literature
Matthew A. Kearney, Brigham Young University Germanic and Slavic Amid the chaos and anticipative excitement of fin-de-siecle Europe, German society quickly became a historical hotspot of unique political and social transformations. It was felt to be a period of degeneration, but at the same time a period of hope for a new beginning. In the midst of a culture that was still perpetuating lively, discriminating social appetites, it is intriguing to note that a broad examination of texts written by German women at this time, which experiment with the very question of social walls and new decisions, shows that these women most often employed conclusions laden with themes of madness, chaos, loneliness and suicide. For this research I have chosen three significant texts which can be viewed from historical and anthropological standpoints on the subject of traditional walls and choices. These are Jenseits der Mauer by Elisabeth Heinroth, Meine Freundin by Hermione von Preuschen-Telmann, and Helene Monbart-Kessler’s Kameraden. The approach to these works, and for the purpose of my research, evaluates the female literary characters in these texts in light of Friedrich Nietzsche’s philosophical view on the fragile balance between the Apollonian and Dionysian drives inherent in art and in life itself. Nietzsche asserted that in life there rages a constant battle between these two major forces, each striving to control the existence of men and women alike. Against the background of this aesthetic discourse, the three texts I have chosen acquire a new dimension: they become an avenue for examining the causes of balance, or lack thereof, in the lives of these female characters. I believe this evaluation is significant because it foregrounds some of the choices and limitations which many women faced as traditional society was changing its attitudes towards them during the late nineteenth and early twentieth centuries.