2024 Abstracts
How English verbs are borrowed into Jordanian Arabic: morphological integration and social perception
Authors: Hannah Smith. Mentors: Jeff Parker. Insitution: Brigham Young University. The Arabic language uses a rich system of patterns called verb forms to create verbs with related meanings from roots made of consonants. For example, the root k-t-b, in form I, kataba, means ‘to write.’ When it appears in form II as kattaba, it means ‘to make someone write.’ In form III, kaataba, it means ‘to exchange letters,’ ‘to correspond’ (Wehr, 2019). There are ten commonly used verb forms, although not every root is used in every form. As Arabic has been exposed to English and speakers borrow verbs from English into their Arabic, they make choices about how and whether to integrate these foreign words into the verb forms. For example, the verb ‘to block (on social media),’ with the derived root b-l-k, is used in form II, ballaka, ‘to block.’ Other borrowed verbs, however, don’t get integrated in this way. Instead, they are used as a noun with a native Arabic verb, usually ‘amila, ‘to do.’ For example, English ‘to delete’ becomes ‘amila daliit, literally ‘to do a delete.’ This study aims to understand what factors influence when and how completely borrowings from English like these are integrated into the verb forms in Jordanian Arabic (JA) by interviewing university students in Amman, Jordan. The interviews are primarily focused on four forms in which borrowings in JA frequently appear (Salem, 2015). Initial data from a group of eight participants shows a general acceptance for the borrowings in their base forms, with much more variation in speakers’ intuitions regarding their usage in other forms. For example, all eight participants perceive ‘to block,’ ballaka, as a completely acceptable word used by most people. However, only two participants felt the same way about its counterpart form, taballaka, ‘to be blocked,’ whereas two other participants perceived it as something no one would ever use. Furthermore, some participants perceive verbs that rely on ‘to do,’ such as ‘to delete,’ to be in the beginning stages of integration, with three of eight interviewees stating that some of these verbs actually are acceptable in verb form II. Thus far, the perceptions of all the borrowings seem to indicate that speakers believe that integration of borrowed verbs is a gradual process. Furthermore, in addition to other factors, the extent to which the verbs are integrated in JA seems to be affected in large part by cultural perceptions of how foreign the verbs are, a factor that has not been noted in previous studies on the subject.
How substrate type influences the germination of Ocimum basilicum
Authors: Emily Cherrington, Sam S Ingram, Chloe E Loveland, Ryanne Welch. Mentors: Olga Kopp. Insitution: Utah Valley University. Ocimum basilicum, commonly known as Genovese basil, is a frequently grown herb in the Lamiaceae family. It is used in food cuisines around the globe and is easily grown as a fresh-market herb for restaurants, farmer’s markets, and grocery stores. Basil has many antioxidants and anti-bacterial properties, but it is most commonly used as a culinary ingredient to add flavor to various dishes. Basil is very sensitive to cold, requiring well-draining soil with temperatures of 18°C to 27°C for germination. Consistent soil moisture is also necessary for optimum growth, and it prefers to stay moist to keep from drying out. In the face of global climate change, it becomes imperative to determine the adaptability of crucial agricultural crops -like basil- to varying nutrient levels in different substrate types across diverse environmental conditions. A plant's capacity to thrive in distinct substrates is intrinsically linked to the unique requirements of its seeds. The choice of substrate serves as the foundational element for cultivating healthy plants. This study centers on examining three pivotal aspects related to substrates: moisture retention, nutrient accessibility, and fungal growth. This led us to question whether the germination success of Ocimum basilicum differs depending on the substrate type. To test this question, we will plant Genovese basil seeds in four distinct substrate types: standard germination mix, 80/20 peat-perlite blend, compost-enriched soil, and an agar medium. Following their placement in the respective substrates, all seeds will be grown in a controlled growth chamber, subject to identical lighting conditions and a consistent temperature of 22°C to ensure uniformity across all treatments. At intervals of 1-2 days, diligent observations will be made to detect signs of germination. Once germination becomes apparent, we will meticulously document the results. Subsequently, this collected data will undergo analysis using the R Language. We anticipate that substrates rich in nutrients (specifically agar and standard germination mix) will exhibit heightened germination success. Investigating how substrate type affects the germination success of Genovese basil offers valuable insights with broad applications in horticulture and agriculture. It can enhance agricultural practices by pinpointing optimal soil choices for specific crops, increasing substrate efficiency to minimize resource wastage, and promoting sustainable and economical cultivation methods. Furthermore, this research contributes to a deeper comprehension of the environmental ramifications of crops and the diversity of crops in both controlled laboratory settings and natural environments.
How the Media Portrays Physical Disabilities
Authors: Jessica Anderson. Mentors: Jennifer Kohler. Insitution: Utah Tech University. Social media, television, and Cinema all play key roles in shaping our perception of reality. This study examines stigmas and stereotypes of disabilities that are perpetuated through the media specifically through these three sources. It also examines the presence of inspiration porn and the proper representation of physical disabilities in these media sources.
Hyperglycemic Conditions Impair Essential Nkx6.1 Expression in Beta Cells
Authors: Jared Wieland, Jacob Herring. Mentors: Jeffery Tessem. Insitution: Brigham Young University. A central attribute of Type 2 Diabetes (T2D) is beta cell damage. This damage commonly affects beta cell’s ability to secrete insulin and regulate blood glucose levels. Nkx6.1 is a beta cell transcription factor essential for proliferation, differentiation, and glucose-stimulated insulin secretion. Hyperglycemia is detrimental to beta cell function and function. We hypothesize that hyperglycemia may negatively affect the expression and activity of beta cell transcription factors, including Nkx6.1. To provide an understanding of the effects of hyperglycemic conditions on Nkx6.1 expression, INS-1 832/13 beta cells were cultured in hyperglycemic conditions, then primary rat islets were treated for the same durations of time. Here, we present transcriptional, translational, cellular localization, and degradation of states of Nkx6.1 over 48 hours of hyperglycemic culture conditions both in vitro and ex vivo models. Comprehension of the mechanisms involved in hyperglycemic downregulation of Nkx6.1 is imperative to the development of treatments for diabetes.
IBD Support: Mental Health Matters
Authors: Kacey Rowe, Rachel Arocho, Allison Ensign. Mentors: Rachel Arocho, Allison Ensign. Insitution: Utah Valley University. Mental health amongst IBD patients is often overlooked and forgotten when they are dealing with their personal physical symptoms. There is little research on the benefits of social support for IBD patients. In online forums, it appears some IBD patients report feeling depressed and having negative thoughts about living life on a day-to-day basis. Indeed, prior research has found associations between IBD and mental health; for example, one study found that 17.3% of IBD patients had lifetime suicidal ideation, which is much higher than the general population at a 4.0% rate (Xiong et al., 2022). Another study did physical and mental exams and found on average, people with higher depression symptoms were six times more likely to have their disease be more active (Marrie et al., 2021, para. 16). Due to the high rate of depression, it would be beneficial to design a website to track symptoms and create online forums giving people the opportunity to connect with peers who have IBD. IBD can drastically affect a person’s life, even in a mild case. According to the CDC, IBD is estimated to affect 3 million US adults, a little over 1% of all Americans (Xu et al, 2018). Few people in the 1% category who struggle with IBD have connected online with others who are experiencing the same autoimmune disease. Although prior research has identified the association between physical IBD symptoms and mental health, little has been proposed to help these patients. Our goal is to create a form of social support for people who have been diagnosed with IBD. For 30 days, we will take 20 participants and have them record their symptoms and thoughts through quantitative and qualitative data. To do this, the participants will be asked to use a website which we have created from scratch. They will be using the Patient Health Questionnaire-9 (PHQ-9) and the Short Inflammatory Bowel Disease Questionnaire (SIBDQ). The PHQ-9 will be used to check in on mental health, while the SIBDQ will focus on the physical symptoms of IBD. Each week, they will participate in forums on the website, which will allow them to connect online and anonymously with others who share their experiences. At the start of the month, daily throughout the study, and at the end of the month, participants will report on their health symptoms through a combination of the measures named above and short tracker questions. (Note: Project is still under IRB approval, we aim to start participants by the end of the year.)
Identification of an aphid-transmitted DNA virus in Brassica oleracea in Utah
Authors: Travis Christensen, Geoff Zahn, Alma Laney. Mentors: Alma Laney. Insitution: Utah Valley University. In July 2022, green cabbage plants (Brassica oleracea) were observed with severe viral symptoms in a local community garden in Utah County. These plants were observed to be covered with cabbage aphids (Brevicoryne brassicae). Symptoms observed on the cabbage plants included yellowing, necrotic lesions, and brittle necrosis of the leaves. These symptoms have been found to be transmissible by the cabbage aphid to broccoli, cabbage, and kale, although symptoms are delayed in kale. Work is being done to find other transmissible plants within the brassica family. Sequencing of RNA yielded no results, however, amplification of viral DNA via rolling circle amplification (RCA) has yielded positive results. A sample obtained through maintenance of the virus and cabbage aphid is currently being subjected to RNAseq using the NovaSeq Illumina platform. Through several virological methods we are able to conclude the presence of a putative geminivirus transmissible through cabbage aphid in our affected plants. To our knowledge, this is the first report of an aphid-transmissible DNA virus of brassicas in Utah. Further work is being done to further identify, classify, and build the genome of this specific virus using Illumina sequencing.
Identification of Novel Viruses Infecting Utah Oil-Bearing Plants Hyssopus officinalis and Lavandula angustifolia
Authors: Megan Frisby, Alma Laney, Geoff Zahn. Mentors: Alma Laney. Insitution: Utah Valley University. The oil-bearing species, Hyssopus officinalis (hyssop) and Lavandula angustifolia (lavender) are important ecological, ornamental, and agriculturally cultivated plants in the state of Utah, playing a central role in the state’s major essential oils industry, and thus economic climate. However, little is known about the virological agents infecting Utah plant species. A project was initiated to identify the viruses infecting symptomatic H. officinalis and L. angustifolia specimens obtained from Utah. Tissues collected from plants of both species exhibited viral associated symptoms of stunting and chlorosis, while collected hyssop specimens displayed additional leaf curling and mottling. Initial MinION sequencing was conducted on cDNA libraries synthesized from lavender and hyssop samples which were multiplexed with that of other species using the cDNA PCR barcoding kit and chip v 9.4.1. The primary run yielded contigs for a novel single-stranded negative-sense RNA nucleorhabdovirus in both specimens, a sequence closest to tomato yellow leaf curl China virus (TYLCCNV) in hyssop, and an ilarvirus related to tobacco streak virus in lavender. However, additional, exclusive MinION runs consistently failed to yield quality results in attempts to obtain the complete the genomes of these viruses. DNA and RNA extractions were once again performed on hyssop and lavender samples, and RNA from 2020 and 2023 were sent out of lab for RNAseq using the Illumina Novaseq. Once complete genomes are achieved further research will be conducted to characterize these novel viral agents, determine the identity of their associated vectors and calculate host ranges. The results of this project will help to advance the limited virological knowledge of Utah plants as well as that of the nation and world, and aid in developing future scientifically-sound pathogen management strategies.
Identifying and Monitoring Groundwater and Surface Water Interactions Utilizing UAVs and Remote Sensing Applications in Wetlands in Utah Lake, Utah
Authors: Anna Wilkinson, Feyan Hoffman, Roshan Abraham. Mentors: Daren Nelson. Insitution: Utah Valley University. Remote sensing technologies, involving drone and satellite imagery, have recently begun to be used in hydrologic research with various applications. The technology is relatively easy to obtain, is noninvasive, and helps minimize cost, access, and safety concerns related to the study of wetlands. The thermal and multispectral imagery can provide unique datasets that expand understanding into groundwater and surface water interactions. As part of this project, we are exploring remote sensing applications within the hydrology of wetlands surrounding Utah Lake, a large freshwater lake within Utah Valley. The project has involved the collection of thermal and multispectral drone imagery from 2021 to 2023. Thermal imagery collected in these areas has assisted in the determining of hot and cold spring locations and how these springs have shifted due to changes in groundwater levels and lake levels. In addition, this imagery has shown how physical anthropogenic influences have also impacted the location and stream channels associated with these springs. Multispectral imagery is used to understand the health of phreatophytes near the lake during dry and wet years to be used as an indicator for groundwater level changes. Even though much of the data is still preliminary and part of a long-term study these remote sensing technologies are already showing how these groundwater systems adapt to seasonal and anthropogenic changes.
Identifying and photographing the instars of a parthenogenetic mayfly (Centroptilum trianguilfer; Ephemeroptera)
Authors: Heidi Kipp, Annika Jones, . T. Heath Ogden. Mentors: T. Heath Ogden. Insitution: Utah Valley University. The purpose of this research is to identify and document mayfly instars from a parthenogenetic population of individuals being raised in the lab. Instars are the developmental stages of an arthropod between molts. Mayflies (and other non holometabolous insects) go through incomplete metamorphosis, moving from egg to nymph stages, and then to subimago (unique to mayflies), and imago (or adult). Previous research indicates the number of instars to be between 12-45 depending on the species, and the species we studied is estimated to have around 15. During each instar, mayflies shed their exoskeleton. The difference between each instar is vital to understanding the changes in gene expression and overall life cycle of a species. Instars have subtle differences, and identifying them will help further research into which genes are involved in producing specific morphological characteristics. Defining these structures, such as the forming of eyes or wings, are of particular interest in evolutionary phylogenetics. We raised Centroptilum trianguilfer (Ephemeroptera: Baetidae) in a controlled water tank, collecting and dating egg samples from mature imagos. Water is maintained with standard ion composition with 130-400 conductivity. Once the eggs hatched, we performed daily inspections and measurements using a 40x stereomicroscope microscope and Lumenera Infinity Analyze. We are using basic appearance, size and morphology) to separate instars. We will identify and photograph each instar. Samples from the different instars, subimagos, and adults will be acquired and used to generate transcriptome data. We propose that our research will aid in the investigation of mayfly evolution and their genetic mechanisms of control.
Identifying the Interactomes of Disease-Causing CryAB Variants
Authors: Joshua Evans, Allison Voyles, McKenzie Bellon, Julianne Grose. Mentors: Julianne Grose. Insitution: Brigham Young University. Alpha-crystallin B (CryAB) is a small heat shock protein that acts as a molecular chaperone and plays an essential role in cytoskeletal organization and myofibril function. Human mutations in CryAB have been associated with various diseases, such as cardiomyopathy and cataracts. However, the precise molecular pathways and protein substrates of CryAB are not yet fully understood and require further investigation. This project aims to increase understanding of CryAB by determining proteins that bind wild-type versus disease-causing variants using yeast two-hybrid screens. It also involves testing for binding specificity of variant-binding partners. A series of these Y2H screens gives valuable information regarding the binding patterns of CryAB, showing distinct binding partners for different alleles of CryAB. Overall, the project provides greater insight into the molecular functions of CryAB as well as a better understanding of the dysfunctional pathways of its disease-causing variants—a factor which may, in the future, have potential applications to the treatment of related diseases in a clinical setting
Image segmentation for breast cancer focused ultrasound therapies
Authors: Taylor Forbes, Rachel Harris, Benjamin Jackson, Nicole Peterson, Sydney Tanner, Chloe Nelson. Mentors: Christopher Dillon. Insitution: Brigham Young University. Focused ultrasound (FUS) therapy is a non-invasive therapy for breast cancer. Treatment plans for this therapy are created on a patient-to-patient basis, which requires a significant amount of time from medical professionals. An important and time-consuming part of developing treatment plans is the precise segmentation of the breast magnetic resonance imaging (MRI) scan and subsequent treatment simulation to ensure that the treatment is effective and safe. Segmentation involves dividing the MRI dataset into segments by assigning distinct tissue types that are then assigned properties and used in simulations to help clinicians plan FUS treatments. However, imprecise interfaces between different tissue types in MRI images lead to discrepancies between individual segmentations, thereby introducing variability into the segmentation process. This variability—which is found even among expertly performed segmentations—can lead to differences in treatment plans. Here, analysis was performed in order to quantify interobserver variability in breast MRI segmentation. This study was conducted by providing basic segmentation training to undergraduate research assistants with no prior segmentation experience. Each participant segmented the same breast MRI dataset into different tissue types using the software Seg3D. The different segmentations were then compared using contour similarity metrics (such as the Dice Similarity Coefficient and Jaccard Index) as well as tissue volume differences. The interobserver variability was quantified using the results from these analyses, which will be helpful in determining the level of precision required for the use of a given segmentation in FUS treatment planning.
Impact of Athletics United
Authors: Alyssa Taylor. Mentors: Dr. Marquez-Velarde . Insitution: Utah State University. Athletics United (AU) is a non-profit organization in Logan, Utah dedicated to using “sport to provide a supportive environment for new American families to integrate with local communities for personal, physical and social growth” (https://www.athleticsunited.us/). To this point, AU lacks meaningful research to measure its progress in meeting its mission statement. In order to qualify and quantify AU’s impact, this project investigates question: “Does Athletics United help families integrate into local communities and how?” Athletics United integrates families into the community by providing a space to build friendships, expand one’s world view, and access community resources. During Summer of 2023, nine families participating in AU were interviewed. A mix of American and new American families were recruited. Four interviewees required an interpreter. Interviews are being qualitatively analyzed using NVivo. The interview data confirms that Athletics United integrates families into the community by providing a space to build friendships, expand one’s world view, and access community resources. To further improve, AU can consider assigning volunteers to help new American families participate in events and access resources more consistently.
Impact of Inducible Depletion of Sequestosome-1 (p62) From Endothelial Cells On Intrinsic Arterial Stiffness
Authors: G. Colton Gardner, Lynasi Gapelu, Sohom Mookherjee, Sihem Boudina, J. David Symons. Mentors: J. David Symons. Insitution: University of Utah. With the prevalence of cardiovascular disease increasing world wide and its precise mechanisms remaining elusive, the need for novel treatments is urgent. Autophagy is a conserved process whereby dysregulated intracellular proteins are escorted to the lysosome for recycling. Emerging evidence indicates that autophagy is important in maintaining endothelial cell (EC) proteostasis and function. My laboratory showed that autophagy repression in adult mice ECs produces arterial dysfunction, and indexes of autophagy are repressed in ECs of older mice and humans which display concurrent EC dysfunction. Sequestosome 1 (p62) tethers proteins and enables their entry into the autophagic pathway. The contribution from EC p62 to cardiovascular and metabolic function is unknown and experiments are needed to investigate this. Thus, p62 will be depleted from ECs of adult male and female mice (p62iEC-/-) via tamoxifen regimen, and results will be compared to animals wherein EC p62 is intact (CON). The efficacy and specificity of EC p62 depletion will be evaluated in ECs and vascular smooth muscle by qPCR and immunoblotting. Three adjacent 1 mm thoracic aorta sections will be obtained 14 days after the tamoxifen regimen. The central aorta section will be used to measure intrinsic mechanical stiffness by wire myography, with the other two used to find lumen diameter and intimal medial thickness. These measurements will help create Stress vs. Strain curves and the Elastic Modulus, which will be compared between the groups to show the differences caused by p62 depletion. We expect p62 depletion from ECs to increase intrinsic aortic stiffness.
Impact of the Removal of CD6 on T Cell Metabolism
Authors: Alexa Bailey, Sophie Daines, Joshua Bennett, Carlos Moreno, Scott Weber. Mentors: Scott Weber. Insitution: Brigham Young University. As the understanding of cancer pathophysiology continues to increase, there is an increased focus on the role of T cells in targeting cancerous cells and tumor sites. Recently, it was demonstrated that CD5, a transmembrane glycoprotein found on T cells, plays a key role in negatively regulating T cell activity. CD5 also significantly impacts T cell metabolic profiles by increasing oxygen consumption rates (OCR) and extracellular acidification rates (ECAR). We are now investigating the role of CD6, a related membrane protein found on T cells, and its effect on regulating T cell proliferation and metabolism by measuring the proliferation rates and metabolic profile of T cells from a CD5/CD6 double knockout murine model. Because tumor microenvironments are vitally important in determining the immune system’s response to cancer, differences in metabolism may yield new insights for developing immunotherapy techniques.
Impact Resistance of 3D-Printed Architectured Sandwich Composite Panels for Future Mobility Solutions
Authors: Ethan Layne, Tareq Hassan. Mentors: Juhyeong Lee. Insitution: Utah State University. A key design criterion for aerospace structural applications is specific mechanical property (i.e., mechanical property divided by the density of a material). Honeycomb sandwich panels which are commonly used in aerospace/aviation structural applications provide lightweight performance, however they have several drawbacks. They include (1) limited alteration of core geometric parameters, (2) few core material selections, and (3) a closed-cell core network. These limitations may be bypassed with 3D-printed lattice-core sandwich panels to provide customizable structural performance. This study investigates impact resistance of architectured sandwich panels designed with various core designs and infill densities. A series of 5~20J low-velocity impact (LVI) tests will be performed on 3D-printed ABS sandwich panels with honeycomb, gyroid, and triangle cores; with infill density varying from 5% to 15%. In this work, the effects of core geometry and corresponding infill density on LVI resistances will be studied to optimize the structural performance of 3D-printed ABS sandwich panels. The primary objective of this study is to characterize these novel sandwich structures with highly customizable 3D-printed complex cores, offering tailorable structure performance.
Impacts of an Intercultural Teaching Experience in the Navajo Nation on Preservice Teachers
Authors: Arahi Perdomo, Brooklyn Moore, Erin Kerr, Hula Santiago, Kylie Kay, Sarahi Joyner. Mentors: Krista Ruggles. Insitution: Utah Valley University. This research initiative centers on the experiences and learning outcomes of preservice teachers (PSTs) from Utah Valley University who volunteer to teach in the San Juan School District (SJSD) and the Navajo Nation. Our primary aim is to understand how this cross-cultural teaching experience shapes their future teaching practices, perspectives on diversity, and inclusion in education.Preservice teachers play a critical role in the future of education This study examines how their participation in a unique cultural context can enhance their preparedness as educators. By engaging in volunteer teaching assignments within the Navajo Nation, these students gain exposure to diverse cultural backgrounds; fostering cultural awareness and sensitivity.We seek to show the transformative impact of this experience on their personal and professional growth. Through interviews and observations, we will explore the challenges and opportunities faced by preservice teachers and how this experience influences their teaching philosophies and aspirations.Our ultimate goal is to determine how such immersive experiences can contribute to the development of culturally responsive pedagogy. We believe that these preservice teachers, with their enriched perspectives, can become advocates for diversity and inclusion not only within their classrooms but also within the broader educational landscape and society.This research holds the potential to inform teacher education programs and policy discussions regarding the value of cross-cultural experiences in teacher preparation. By shedding light on the impact of volunteer teaching in the Navajo Nation, we hope to contribute to the ongoing dialogue on fostering more inclusive and culturally aware educators, ultimately benefiting students and society as a whole.
Implementation of Culturally Relevant Teaching in Private Studios
Authors: Aullora Fekete. Mentors: Karen Jensen. Insitution: Brigham Young University. Implementation of culturally relevant teaching in universities and public schools has increased, but private studios, outnumbering both higher education and public school dance programs, are not keeping pace. Business owners are caught between competing demands making it difficult to invest in professional development programs for their instructors(Risner 2010). This perpetuates implicit bias teaching as instructors fall back on how they were taught in studios based in eurocentered evaluation and ideals furthering the divide for who has accessibility to dance and which dance forms are taught.The following research addresses possible ways private studios can have more access to and receive training for more culturally relevant teaching. Conversation explores how studio vision and clientele demands can be taken into account to create a more inclusive dance culture and the types of training resources already offered along with how they might be implemented. By having an inclusive approach, studio dancers also continue the culturally relevant teaching they received as future dance educators to contribute to a greater dance culture of uplifting and including the marginalized. Citation:Doug Risner Ph.D. M. F. A. (2010) Dance Education Matters: Rebuilding Postsecondary DanceEducation for Twenty-First Century Relevance and Resonance, Journal of Dance Education, 10:4, 95-110, DOI:10.1080/15290824.2010.529761
Implementing Quality Control for the Fluorescent Coating of Intravenous Catheters
Authors: Melissa Wiggins, Aaron Bigelow, Porter Ellis. Mentors: Ronald Sims. Insitution: Utah State University. Ensuring that the hydrophilic coating of Merit Medical’s Prelude IDeal trans-radial catheter is necessary for its biocompatibility and patient safety. The current method for testing the coating involves a test using Congo Red Dye. The Congo Red Dye does work, but the dye is toxic and all tested catheters must be discarded after testing. The Conge Red Dye test results in wasted catheters. A new method for testing the coating uses fluorescent particles. First, fluorescent particles are added to the hydrophilic coating. These fluorescent particles can be easily visualized on the catheter using UV light. Thus, the uncoated portions of the catheter can be visualized as well. The coated catheters are placed into a black box that ensures only the catheter is being seen. A line scan camera is used to take pictures of every side of the catheter as the catheter spins. Photos of the scanned catheter are then linked together, showing the entire circumference in one picture. The catheter is ultimately tested by analyzing the full picture to find any uncoated regions. By using software to analyze the full picture, the size of uncoated regions is determined with greater accuracy. This new method allows for tested catheters to be used after testing and does not involve any toxic chemicals.