Authors: Megan Frisby, Alma Laney, Geoff Zahn
Mentors: Alma Laney
Insitution: Utah Valley University
The oil-bearing species, Hyssopus officinalis (hyssop) and Lavandula angustifolia (lavender) are important ecological, ornamental, and agriculturally cultivated plants in the state of Utah, playing a central role in the state’s major essential oils industry, and thus economic climate. However, little is known about the virological agents infecting Utah plant species. A project was initiated to identify the viruses infecting symptomatic H. officinalis and L. angustifolia specimens obtained from Utah. Tissues collected from plants of both species exhibited viral associated symptoms of stunting and chlorosis, while collected hyssop specimens displayed additional leaf curling and mottling. Initial MinION sequencing was conducted on cDNA libraries synthesized from lavender and hyssop samples which were multiplexed with that of other species using the cDNA PCR barcoding kit and chip v 9.4.1. The primary run yielded contigs for a novel single-stranded negative-sense RNA nucleorhabdovirus in both specimens, a sequence closest to tomato yellow leaf curl China virus (TYLCCNV) in hyssop, and an ilarvirus related to tobacco streak virus in lavender. However, additional, exclusive MinION runs consistently failed to yield quality results in attempts to obtain the complete the genomes of these viruses. DNA and RNA extractions were once again performed on hyssop and lavender samples, and RNA from 2020 and 2023 were sent out of lab for RNAseq using the Illumina Novaseq. Once complete genomes are achieved further research will be conducted to characterize these novel viral agents, determine the identity of their associated vectors and calculate host ranges. The results of this project will help to advance the limited virological knowledge of Utah plants as well as that of the nation and world, and aid in developing future scientifically-sound pathogen management strategies.