Architecture

Authors: Karen Hoggan, Adia Migliori, Jonathan Harr, David Aguilar Alvarez. Mentors: David Aguilar Alvarez. Insitution: Weber State University. BackgroundElevated blood pressure (BP) is a significant risk factor for cardiovascular disease. The effects of high sodium (Na) intake on blood pressure are widely recognized. Of late, potassium (K) intake has gained recognition as a factor in hypertension. Recent studies have shown that the Na/K ratio consumed may be more predictive of hypertension than solely sodium intake. This study examines the association between the Na/K ratio and BP in males and females.MethodsWe utilized a cross-sectional design to evaluate self-reported two-day diet records for 73 male and 183 female students. Sodium and potassium intake were calculated using Diet and Wellness Plus. BP measurements were taken from each participant. Partial correlations were conducted between systolic (SBP) and diastolic blood pressure (DBP), and the following variables: Na, K, and Na/K. SBP means were compared between high sodium consumers and low sodium consumers with the same Na/K ratio using independent samples t-test.ResultsNeither male nor female participants’ SBP or DBP were associated with Na or K intake (p>0.05). However, an increased Na/K ratio was associated with higher DBP in males (r=0.254, p=0.29), but not in females (r=0.04 p=0.79). When the Na/K ratio was paired, participants who consumed over 3000 mg of sodium had a significantly higher SBP (121.1 ± 12.9) than those who consumed less than 3000 mg (115.9 ± 10.7, p=.004).ConclusionsConsumption of Na and K may affect male and female BP differently. In males, the Na/K ratio was predictive of increases in blood pressure. Although Na/K ratio seems to be more predictive of this parameter, within the same Na/K ratio, having lower sodium consumption was associated with lower SBP. This illustrates the complex interaction between BP regulation and nutrition, emphasizing the significance of both lowering sodium intake and maintaining a balanced Na/K ratio for optimal heart health.

Authors: Sophia Kramer, Violeta Vasilevska. Mentors: Violeta Vasilevska. Insitution: Utah Valley University. Preliminary literature review showed that studies have been conducted following either elementary or middle/high school students’ after-school math programs. However, this preliminary lit review did not provide any relevant comparison of the data collected from these students’ groups. In this study, we will conduct two after-school meetings with elementary and high school students in the spring semester. During the meetings, students will be working collaboratively on hands-on, discovery math activities. This hands-on learning experience will be adjusted for different grade levels. Both student groups will be given a pre-survey consisting of attitude questions. They will assess perceptions about learning that occurs in after-school (out-of-classroom) learning environments that encourage mathematical thinking and collaboration. At the end of each after-school meeting, students will be given a post-survey (that will contain some questions matching the pre-survey ones). The collected survey data will be compared to examine the different responses resulting from the two different age groups, maturity levels, gender, etc. Survey results will be used to obtain observations about the effect of such after-school programs on student attitude toward math.In this presentation, we present the preliminary data collected during the first after school meeting held at the beginning of the spring semester and provide a comparison of the collected data. Additional meetings and surveys will be conducted later in the semester.

Authors: Eden Backman, Britt Wyatt, Kandace Flanary, Jessica Cusick, T Heath Ogden, Joshua Premo. Mentors: Joshua Premo. Insitution: Utah Valley University. Utah Valley University serves a unique student population where ~70% of students are Latter-Day Saints (LDS) and ~30% are non-traditional (over the age of 24) (UVU IR, 2023). Compared to the overall 1.6% of LDS individuals in the United States (Pew Research Center, 2023), the identities of UVU students are vastly underrepresented in education research that has been conducted at other institutions. This is especially important as the LDS religion has been related to one of the most conservative religious groups concerning gender roles (Heaton, Bahr, and Jacobson 2004). Additionally, recent preliminary analysis of public institutional data from Utah universities indicates that UVU is graduating more men than women in biology, drastically contrasting other Utah university and national trends. In Utah, the STEM workforce has seen a significant 20% increase since 2016 (UWLP, 2022). However, it's important to note that during this period of expansion, men have disproportionately reaped the benefits in terms of STEM career opportunities. According to IWPR (2019), men in Utah are 2.5 times more likely to secure a STEM career compared to women. Furthermore, in 2020, STEM careers in Utah boasted median annual wages that were over twice as high as those in other occupations, which has direct implications for individuals' lifetime earnings and social capital. Given this information, we questioned why women were not choosing educational paths that will promote a career in STEM? If they are choosing STEM majors, then what are the factors that promote women to leave STEM during their undergraduate careers? This research project focuses on trying to answer these questions specifically at Utah Valley University (UVU). UVU’s biology program reflects similar patterns that are seen in the STEM workforce in Utah. UVU’s biology program was graduating twice as many men compared to women during the most recent academic year (2022-23)(UVU IR). This was the lowest percentage of women earning a four-year biology degree when compared to all other Utah institutions (USHE, 2023). The following research questions were addressed to help inform our understanding of why inequities in degree attainment by gender are being seen at UVU: 1) Are women less accepting of biological ideas (evolution, vaccines, climate change, etc.) that may be impacting women’s pursuits of biology? 2) Are there differences in how women are relating to science (motivation, immersion, or integration) that may be impacting their pursuit of biology? 3) Are there differences in political orientation or religious affiliation that may be impacting women’s pursuits of biology? To answer these questions, we collected data from 1,455 students (22% were biology majors) enrolled in biology classes at UVU in Fall 2023. Comparisons were made using independent samples t-test (with Bonferroni adjustments) to see the extent to which factors varied between men and women both within and outside of the biology major. In contrast to expectations, results for research question #1 show that women biology majors did not agree with science less than men. In fact, women strongly agreed with several science ideas at a higher rate than did men. These ideas included environmental concern and climate change (women = 66% vs. Men = 41%) and vaccination (women = 63% vs. Men = 48%). This result suggests that differences in acceptance of science ideas may not be driving the differences in degree attainment in biology. If anything, these results suggest that women should be more likely to continue in the biology major compared to men. In addition, when students’ immersion (sense of belonging and science identity) in science was assessed by gender women were slightly higher than men with about 8% more strongly agreeing to being immersed in science. This finding suggested that women are not experiencing lower immersion as a potential factor impacting degree attainment. Further examination of additional factors is underway. This study highlights that there are specific factors that may be contributing to gender disparities in biology bachelor's degree attainment at UVU. We recommend specific interventions targeting areas of differences in the biology program to provide additional scaffolds and supports to promote more equitable career trajectory and lifetime earnings for women pursuing Biology at UVU.

Authors: Tessa Sabin, Derek Bagley, Kylie Olsen, Alexis Klein, Haven Broadhurst. Mentors: Sandi Gillam. Insitution: Utah State University. The purpose of this project was to create systematic, reliable rubrics for using the Frog Story series in language sample analysis for the diagnosis and treatment of children with developmental language disorders (DLD).Language sample analysis is a critical part of the assessment process by speech language pathologists (SLPs) for determining whether a child has a developmental language disorder. This is accomplished by analyzing a child’s use of vocabulary, sentence structures, and grammatical markers (i.e., past tense) and comparing it to their typically developing peers. Once diagnosed, language sampling is used as part of progress monitoring efforts to ensure the child is making reasonable progress toward their language goals in one (or all) of these areas.Elicitation of language from a child is often achieved by asking them to tell a story. One popular way that SLPs have obtained samples is to ask them to retell one of four popular wordless picture books from the Frog Story series by Mercer Mayer. This series involves the antics of a frog and his boy as they encounter different adventures. There is no rubric or “analysis key” associated with the stories leaving the SLP to decide how to use the information obtained from the story independently. This makes it hard to obtain reliable results over multiple time points for use in progress monitoring.In this project, a team analyzed all four Frog stories for their inclusion of specific story elements (i.e., character, setting, episode), vocabulary and sentence structures; and separate rubrics were created. The methodology for identifying the language parameters of interest, reliability in coding, and uses for the rubrics will be described. The rubrics will be automated using a web application so clinicians can upload their child’s story and have them instantly scored, making their use in analysis more reliable and consistent.

Authors: Grant Hendrickson, Christian Moody, Nellie Bowman, Nick Jeffery, Cairo Ziebarth, Joel Davis, Melissa Reeves, Heather Wilson Ashworth. Mentors: Heather Wilson Ashworth. Insitution: Utah Valley University. Self-competence and effort belief may impact student motivation and success in introductory chemistry courses. Self-competence is the need to feel efficacious and capable3. Effort belief is the concept that working harder at a task produces better results4. Mastery-Based Grading (MBG), a nonconventional grading method, provides multiple opportunities for students to show mastery of the course learning objectives5. This research examines the effect of Mastery-Based Grading (MBG) in an active learning classroom on student's self-competence and effort belief compared to that of a conventional grading (CG) approach in a traditional lecture-based classroom. Hypothesis: MBG leads to increased student self-competence and effort belief compared to CG courses. Self-determination1 and expectancy-value theories2 provide the context for understanding the self-competence and effort belief data. At a private midwestern college, general chemistry students in an active learning, MBG course (53 students) and a lecture course (48 students) with a conventional grading policy were studied using a mixed methods approach. Surveys containing Likert and reflection questions were given during week 3 and 14 of the semester. A coding scheme was developed, and reflection questions were coded to consensus. Coded data had a Pearsons coefficient greater than 0.76. Results: Notably, students with initially low self-competency Likert scores showed significant improvement (p=0.01) in the MBG-active course, but not in the conventional course. No significant improvement was observed in students' effort-belief scores as the scores were high at the beginning and stayed high at the end of the semester. In response to the reflection question, “Do you believe you have the ability to be successful in this course?”, there was a 15.35 percent increase in responses that reflected effort-belief in the MBG active learning course. This theme was absent in CG courses throughout the semester. It is noteworthy that MBG students recognized (14.29% compared to pre-reflections) the specific components of the course structure that helped students succeed. This early explorative data suggests that students in active learning MBG courses may experience an increase in self-competence and perceived effort belief compared to their counterparts in traditional classes. 1Deci, E. L., Vallerand, R.J., Pelletier, L.G. & Ryan, R.M. (1991). Motivation and education: The self-determination perspective. Educational Psychologist, 26, 325-346. 2Eccles (Parsons), J. (1983). Expectancies, values, and academic behavior. in J.T. Spence (Ed.) Achievement and Achievement Motivation. San Francisco: W.H. Freeman. 3Ferrell, Brent and Jack Barbera. (2017). Analysis of students’ self-efficacy, interest, and effort beliefs in chemistry. Chemistry Education Research and Practice, 16; 31-337. 4Skinner, E., Saxton, E., Currie, C., & Shusterman, G. (2017). A motivational account of the undergraduate experience in science: brief measures of students’ self-system appraisals, engagement in coursework, and identity as a scientist. International Journal of Science Education, 39(17), 2433-2459. 5Toledo, S., & Dubas, J. M. (2017). A learner-centered grading method focused on reaching proficiency with course learning outcomes. Journal of Chemical Education, 94(8), 1043-1050.

Authors: Kira Swann, Cody Dirks. Mentors: Joshua Price. Insitution: Southern Utah University. As students, we have personally noticed that when we had a long-term substitute in the classroom, we didn't learn as much compared to our peers in other classrooms. It has been found in California as well as other states that a teacher’s absence, leaving a substitute in place, leads to worse off standardized scores for students. This paper tests the impact of long-term substitutes on the STAR/CAASPP scores of students. The purpose of this study is to see if there is a detrimental effect on student scores and if long-term subs should be something addressed by the state. Using testing and demographic data from the California Department of Education, we use a fixed effects regression to test the true impact of the impact of long-term substitute teachers. Our results show that there is a small, but statistically significant impact on student performance.

Authors: Kelsey Grimshaw. Mentors: Joshua Price. Insitution: Southern Utah University. Job satisfaction is a multidimensional state resulting from one’s job and their responses to it. Job satisfaction contains eight facets: recognition, working conditions, management, pay, promotions, coworkers, supervision, and the job itself. The concept of economic sectors was first introduced by economist Colin Clark. He suggested industries could be separated into categories. These categories would be determined based on significantly different characteristics of industries. There are currently five economic sectors: primary (raw materials), secondary (manufacturing), tertiary (service), quaternary (information services), and quinary (public service). Data on occupation and job satisfaction from the General Social Survey were used to build a multivariate linear regression. Results are forthcoming.

Authors: Beckham Trigo. Mentors: Johnathan Chidester. Insitution: Southern Utah University. The lack of resources, funding, and federal recognition is leading to the deficit in K-12 Education around the United States compared to other first world countries with developed education systems. For years the United States has been hammered on the fact that the country doesn't provide every child with bilingual study opportunities. European countries such as Spain, Italy, Germany, and France all offer bilingual studies and are making them required for students to take. These are key elements in a successful education system and offer so many more opportunities and benefits than the standard single language system we have placed in our K-12 Schools. Statistically the change to a new bilingual research would greatly benefit our country and would help decrease political divisiveness and bring an extent of unity among several ethnic groups in the country. The country continues to grow through immigration where the United States is projected to become the top Spanish speaking country in the world with 140 million speakers. However, the United States is not preparing for this and investing in its future students and citizens. Research has proven time and time again that there are so many benefits to a bilingual education, some of these including an increase in brain activity, and power in children, an academic advantage, and competition in the job market. The United States has deeply oppressed this system due to systematic racism, and political motivation. However, the United States has no established language in which it defines itself even though the majority speak English. The United States is also a world power with a lot of interaction with foreign countries. This in itself is going to be very problematic for the future of the United States as the world climate changes. It needs leaders to represent itself in many other languages, not just in the English language. The contrary to this research is that the language is ineffective to learning, makes it harder for students to assimilate into the United States culture, and spend too much time learning the second language. However, none of these statements can be statistically backed and solely based on a few percent of people's personal experience. Overall the research time and time again directs the United States to the bilingual education system, but when will it be adopted?

Authors: Lauren Howell. Mentors: Joshua Price. Insitution: Southern Utah University. This paper empirically assesses the relationship between state abortion policies and birth control consumption per state in the United States. The intention is not to advocate for or against abortion, but to explore how abortion policy influences the usage of contraceptives per state. The surveillance of contraceptive use holds paramount importance in preventing unintended pregnancies, with 95% of these occurrences attributed to inconsistent or incorrect contraceptive use or the absence of any method. Understanding how behaviors and contraceptive practices change over time in response to state policy alterations is crucial for evaluating the health implications of healthcare access regulations. Contraception stands among the greatest public health achievements of the 20th century, significantly reducing maternal mortality, benefiting pregnancy spacing for both maternal and child health, enhancing female participation in the workforce, and fostering women’s self-sufficiency. Utilizing data from sources such as the American Community Survey (ACS), the Abortion Patient Survey (APS), the National Survey of Family Growth (NSFG), and the Natality Detail File, this study delves into a comprehensive analysis of contraceptive use in relation to state abortion policy. Through statistical analysis using Stata 18 and ordinary least-squares (OLS) regression, this research aims to build on prior studies and ascertain the relationship between strict state abortion laws and birth control consumption. It hypothesizes that restrictive policies, such as increasing the cost and difficulty of accessing abortion, might incentivize women to adopt more effective contraceptive methods to avert the consequences of unintended pregnancies. The study also references prior research that suggests changes in contraceptive use, notably an increased reliance on long-acting reversible contraception (LARC), and how it has contributed to a decline in abortion rates, particularly among adolescents. The results of this study hold the potential to advocate for an increased educational focus on safe sex practices and to increase the accessibility of family planning resources based on state policies. It aims to shed light on the potential impact of restrictive abortion laws on birth control consumption and encourages informed policy decisions for public health and family planning.

Authors: Allie Baumgartner. Mentors: Chizu Matsubara. Insitution: Utah Tech University. • ABSTRACT: A student’s understanding and comprehension of content specific vocabulary is essential for their learning. However, Multilingual Learners (MLs) can struggle to understand what they are learning when they do not have knowledge of content specific vocabulary. This research focuses on the implementation of strategies into a small group reading lesson. These strategies will work towards increasing students’ understanding of content specific vocabulary words, in the areas of social studies and science. By using the methodology of action research, the research will implement research-based strategies for strengthening vocabulary for students learning English as a target language. This research will be conducted over a six-week time frame, with two focus students that are multilingual learners. The research-based word-learning strategies will be incorporated into the small group consisting of four students, to support their learning. Word-learning strategies are strategies that assist students to ascertain the meanings of words that are unfamiliar to them. Students can then apply these strategies to learning of unfamiliar words that are included in the content reading that they do in another small group. The word-learning strategies will support the students in learning, and understanding the meaning of content specific vocabulary words.

Authors: Luis Valdez, Mary Alvarez, Jessica Berryman, Ron Valcarce. Mentors: Jessica Berryman. Insitution: Salt Lake Community College. Plants produce & emit a diverse and substantial amount of Volatile Organic Compounds (VOCs) into the atmosphere. These compounds play important roles in the plant’s tri-trophic interactions, responses to environmental stress, and have been shown to participate in atmospheric chemistry. Although variation in VOC emissions in plants has been extensively studied, there are gaps in knowledge on how symbiotic interactions with soil microbiota shape the VOC profile of plants in the context of environmental stress. Arbuscular Mycorrhizal Fungi (AMF) are fungi that form symbiotic associations with plants by colonizing their roots & providing enhanced micronutrient & water retention in exchange for photosynthates. These associations have also been shown to enhance plant responses against biotic & abiotic stress. In this study, we will look at how the expression of heat stress- induced VOCs changes based on mycorrhizal availability in the soil. Populus b. trichocarpa saplings will be exposed to the respective temperatures of 25° C, 35° C, & 40° C for an extended period of time. Emitted volatiles will be collected and analyzed using GC-MS. For each type of temperature, emissions will be compared between saplings planted under different levels of AMF availability (low, medium, & high). We expect volatile emissions to change based on mycorrhizal availability in one of two scenarios; increased emissions due to increased phosphorus uptake from the fungus or decreased emissions due to carbon allocation to the fungus.

Authors: Katherine Christensen, Danielle Kemmer. Mentors: Ron Valcarce. Insitution: Salt Lake Community College. Phenytoin is listed by the World Health Organization as an Essential Medicine that is one of the most cost-effective anti-epileptic (AED) treatments available. However, the availability of the drug to pharmacies in developing countries is limited. 85% of those affected with epilepsy live without treatment. Low commercial production, political instabilities, and/or financial barriers prevent the availability of this anti-epileptic drug. A more efficient and cost-effective method for supplying phenytoin to local clinics and medical personnel could alleviate some of these barriers. The initial goal of this project was to refine a small-scale synthesis and purification of phenytoin using the base-catalyzed addition of urea to benzil, followed by pinacol rearrangement and recrystallization. Our procedure emphasized simple laboratory equipment found in the most basic of pharmacy laboratories. Using the International Pharmacopoeia guidelines for pharmaceutical purity, we achieved over 98% purity. Verification of pharmaceutical grade purity was achieved by High-Performance Liquid Chromatography (HPLC). Our Secondary goal was to incorporate a more efficient and accessible synthesis method. This goal was achieved through the implementation of Microwave Assisted Extraction (MAE). This project outlines the comparison between these two methods and the potential benefits and limitations of each of these methodologies.

Authors: Antonio Ruiz Ayala. Mentors: John Flood. Insitution: Salt Lake Community College. Aerogels are a fascinating material that function as great insulators. Nasa has partnered with Aspen Aerogels to produce a commercially viable product that aims to tackle a variety of problems such as space shuttle insulation, space particle collection, thermal safety, and cryogenic applications. The main objective of our project is to reproduce an aerogel blanket using standard lab conditions and chemicals. We will be producing the blanket using the silica gel process. We will compare properties of durability, cost, and thermal conductivity. We plan to pinpoint a procedure that provides the most cost effective, durable, and thermally conductive material under standard lab conditions.

Authors: Christopher Galbraith, Derek Langford, Hillary Wadsworth, Eliza White, Erin Taylor, Lauren Ford. Mentors: Jordan Yorgason. Insitution: Brigham Young University. Microglia are monocyte derived immune cells and exhibit complex signaling behavior that include phagocytic activity to threats and prolonged neuronal activity. ATP (adenosine triphosphate) is a known chemoattractant for microglia, but how chemoattraction is modulated by other transmitters is not well understood. ATP is co-packaged and released with dopamine, thus the present work examines microglia morphology and motility in the context of these two transmitters. Microelectroiontophoresis and multiphoton microscopy were used in brain slices from transgenic mice to examine effects of dopamine and ATP signaling on microglia. Lipopolysaccharide (LPS) transitioned the microglia from ramified to amoeboid morphology over a period of 4 hours. LPS also increased both dopamine and ATP release, as measured by fast scan cyclic voltammetry on a similar time course. Surprisingly, dopamine itself did not act as a chemoattractant to microglia, despite increasing after LPS treatment. By examining this relationship between neuronal and microglial activation we can better understand the complex circuitry of the reward pathway and immune system activation.

Authors: Daniel Luke Isemonger, Jacob Cecil, Noah Moffat, Nathaniel Horne, Jordan Yorgason. Mentors: Jordan Yorgason. Insitution: Brigham Young University. Anxiety disorders are increasingly prevalent, and can be exacerbated by drug use, which can contribute to further drug seeking behavior. The underlying neural mechanisms of this relationship are not fully understood, but dopamine transmission is thought to play a key role. The goal of this project is to develop innovative tools to elucidate the role of dopamine and drugs of abuse on anxiety-like behavior and its relation to drug seeking in mice. Specifically, dopamine sensors and 2-photon microscopy via implanted endoscopic lenses, will be used to image dopamine dynamics in the NAcc of behaving mice while they are engaging in a virtual environment (VR) mimicking drug exposure and stress conditions. Using the simulation, mice are exposed to fearful stimuli, which will be related to behavioral responses (movement distance, speed, direction) and extracellular release events. By combining these techniques, we will be able to gain a deeper understanding of how dopamine terminal signaling contributes to drug seeking. This research has the potential to shed new light on the neural relationship between drugs of abuse and anxiety. This could help to inform the development of novel drugs and treatments for this disorder.

Authors: Andrew Call. Mentors: John Chaston. Insitution: Brigham Young University. The main goal for this project is to confirm previous predictions of specific genes that may influence flies’ dietary preference to consume lactic acid bacteria (LAB). The preference of LAB is a normal phenotype that flies have, because the bacteria is essential for their gut microbiota. A previous genome-wide association study (GWAS) by another student in my mentor’s lab predicted genes that lead flies to prefer to consume diets that have live LAB. This genetically determined fly phenotype was measured by calculating the frequency and number of times the flies would choose the LAB inoculated diet over a control diet. My role will be to test if 7 genes have the influence predicted by this previous analysis. The experiment will follow close with the one previously performed by the student who predicted the genes I’m testing. I’ll be using a flyPAD which has a small arena (cage) that will hold one fly. Inside that arena are 2 wells that contain food. These wells have sensors attached to them, so each time the fly takes a sip of food, an electronic signal will be sent to a computer that keeps track of the number of sips from each well, the duration of each sip taken and time in between each sip and graph all the results taken for comparison between a control group and the test groups. I will starve 48 female mutant drosophila melanogaster for 3 hours prior to the experiment and then place them inside the flyPAD where I have previously placed 1 μl of 1:1 yeast-glucose diets in each well, however I will inoculate 0.05 μl of LAB in one of the diets. I will track each sip the flies take from each of the two foods for 1 hour. To measure accurately, there will be 2 controls also being tested. I will use flies that do not contain the genetic mutation and I will also have control arenas where both food wells do not contain LAB.I expect that any mutant that has a reduced preference for LAB-inoculated diet relative to the control diet represents a validated prediction of the previous study. In some cases, there may be no variation in preference for the control or LAB-inoculated diet, indicating a gene that does not contribute to fly preference for LAB in the diet. If none of the mutants tested validate the genome-wide association predictions, I will go back to the gene list and select one additional subset of genes to test if these influence the genetic prediction. We previously focused on genes that had multiple hits in the previous experiment, regardless of how significant the predictions were. In this second round, I will simply select the ten most significant remaining genes. Overall, this project will help confirm the previously selected genes with their association with a diet preference of lactic acid bacteria, improving the gut microbiota of Drosophila melanogaster.

Authors: Hunter Nelson. Mentors: Jamie Jensen. Insitution: Brigham Young University. Burnout is a mental condition resulting from prolonged stress in work or school. The Healthcare workforce experiences burnout much more than other career fields and many students in undergraduate STEM are pursuing a career in this field. The Pre-Health degree is one of the most competitive and demanding majors in STEM and we expect there to be high levels of burnout amongst these students. Our purpose was to gather and analyze data on the goals and burnout of pre-health care undergraduate students. We used surveys to collect data from students ranging from Freshmen to Seniors. The survey asked students questions regarding their goal motivations and feelings of burnout. Our results display a correlation between mastery-avoidance and experiencing burnout. By understanding these relationships, we can better help pre-health students avoid burnout.

Authors: Rebecca Kreutz. Mentors: John Chaston. Insitution: Brigham Young University. Previous studies of the relationship between Drosophila melanogaster and its microbiome have shown that the bacteria in fruit flies can have a large effect on life history strategy. Higher proportions of Acetobacter, a genus of aerobic bacteria, correlate with faster development to reproductive maturity and higher, earlier fecundity. Greater proportions of Lactobacillus, a genus of anaerobic bacteria, correlate with lower initial reproduction but longer lifespan and maintenance of somatic cells. Despite this, the determinants of a Drosophila’s microbiome are still not well understood. This study will explore the relationship between the depth of the food that flies mature in and the proportion of anaerobic bacteria found in the flies. To test this, I will prepare vials with varying amounts of food, place fly eggs in them, inoculate them with a mixture of Acetobacter and Lactobacillus, homogenize the flies that mature, and compare the proportions of bacteria across the different food depths. Greater food depth will mimic food that is more rotten, because as fruit rots it becomes softer and the fly larvae are able to burrow deeper. As the flies gain greater access to the inside of the fruit, there may be an increase in the proportion of anaerobic bacteria in the fly’s gut, due to the reduced access of the bacteria to the air. Because different food depths can simulate different fruit ages, this study may be able to establish a relationship between the season the flies mature and their microbiome.