Architecture

Authors: Seth Leavitt. Mentors: Nathan Crane. Insitution: Brigham Young University. One common form of 3D printing is Fused filament fabrication (FFF). In this process, a plastic filament is melted and extruded one line at a time to form a 3D shape. FFF often has problems that arise during printing that can cause the rest of the print to fall apart or otherwise fail. We assessed the feasibility of classifying 3D printing errors in prints using Thermography and data processing. We did this by taking constant thermal video of a specially designed printer to collect our temperature data. Then, using a constant travel speed on the printhead and using the distance traveled, we calculated the speed at which the cooling occurs. We collected cooling data on both control prints as well as parts with simulated defects (gaps in the print substrate). By analyzing the differences between the two sets of data, we determined that is feasible to identify anomalies in the printed part. This is a first step towards improving the quality of 3D-printed parts.

Authors: Rachel Krupnek. Mentors: Jie Liu, Sazib Hasan, Vinodh Chellamuthu. Insitution: Utah Tech University. Many groups are interested in potential market indicators because more than 60% of adults in the United States have investments in the stock market. Extreme stock trading volumes can be an important indicator of unusual market events, including those that precede market crashes. Several studies utilized Extreme Value Theory (EVT) and the Generalized Pareto Distribution (GPD) to examine and model the behavior of these extreme volumes. In particular, various estimation techniques are explored within the GPD framework to best fit the stock trading volume data. The goal of this study is to utilize mathematical approaches in economics. Our results have implications in both mathematical and economic fields, providing benefits by enhancing our ability to analyze and prepare for extreme market occurrences.

Authors: Matthew Williams. Mentors: Elena Laricheva. Insitution: Utah Valley University. Protein-ligand docking is a computational method widely used in drug discovery to predict binding affinities of small molecules to target receptors. However, despite its widespread utility, the method has inherent limitations that can lead to false negative and false positive results, affecting its reliability. False positives occur when docking predicts strong binding affinities that experimental evidence does not confirm, while false negatives arise when the method fails to identify potent binders validated in experiments.Various factors contribute to these inaccuracies, including limitations in scoring functions and search algorithms, but a significant issue lies in the neglect of protein dynamics, i.e., receptor flexibility. To address this limitation, flexible docking methods, which partially account for receptor flexibility, have been developed, but they come at a considerable computational cost. In this project, we incorporated molecular dynamics simulations and k-means clustering to improve prediction of binding energies of a series of small molecules to the human dopamine 2 receptor, a crucial therapeutic target for neuropsychiatric disorders.Our findings demonstrate that sampling conformational states through molecular dynamics and clustering, followed by docking to representative clusters, offers a more accurate assessment of binding energies. Remarkably, this enhanced predictive capability is achieved with minimal additional computational expense.

Authors: Heather Smith. Mentors: Vinodh Chellamuthu, Jie Liu, MD Sazib Hasan. Insitution: Utah Tech University. The invasive species Japanese Beetle (Popillia Japonica) poses significant ecological and economic challenges in the United States, with an annual expenditure exceeding $300 million on damage repair and control efforts. The beetle's life cycle predominates as grubs in turf from late August to April, suggesting a strategic window for control interventions. We developed an individual based model of the Japanese Beetle life cycle, utilizing predator-prey models, to demonstrate the effectiveness of various control methods. Our findings would suggest that ideal timing of grub treatments within the diapause stage markedly reduces beetle populations and is a cost-effective control method. The results of this model can inform land management decisions in controlling Japanese Beetles.

Authors: Ben Martinez, Virginia Merrill. Mentors: Muhammed Yildiz. Insitution: Utah Tech University. This study investigates the multifaceted dimensions of food insecurity among students at Utah Tech University, drawing from the American College Health Association National College Health Assessment survey conducted in Spring 2021, comprising a sample size of 347 students. Shockingly, 46.7% experienced food insecurity in the past 30 days, with certain demographics disproportionately affected—specifically, male, nonwhite, non-heterosexual, disabled, athlete, and single students. We also found associations between food insecurity and deteriorating health and well-being. Those affected reported significantly lower overall health and well-being levels, alongside heightened instances of suicidality, stress, and psychological distress. This research underlines the urgency to address food insecurity, particularly among at-risk demographic groups, and emphasizes the critical need for targeted interventions and support mechanisms. It highlights the concerning link between food access and mental health and advocates for inclusive strategies to address disparities among students. Implementing support systems to mitigate food insecurity's adverse impacts is crucial in fostering an environment conducive to the academic success and overall well-being of all students at Utah Tech University.

Authors: G. Colton Gardner, J David Symons, Sohom Mookherjee, Michael Judge, Lynasi Gapelu, Sihem Boudina. Mentors: J. David Symons. Insitution: Utah Valley University. The prevalence of cardiovascular disease (CVD) is higher in residents of rural vs. urban communities, and in older vs. younger individuals. Precise mechanisms responsible for CVD are elusive, and the need for new and novel therapeutic interventions is urgent. Autophagy is a conserved process whereby damaged and dysregulated intracellular proteins are identified, tethered, and escorted to the lysosome for degradation and recycling. Emerging evidence indicates that autophagy is important in maintaining endothelial cell (EC) proteostasis and function. For example, my laboratory showed that: (i) autophagy repression specifically in ECs of adult mice produces arterial dysfunction; and (ii) indexes of autophagy are repressed in ECs of older vs. adult mice and humans that display concurrent EC dysfunction. Sequestosome 1 (p62) tethers proteins targeted for degradation and enables their entry into the autophagic pathway. The contribution from EC p62 to arterial function is unknown. To address this, p62 was depleted from ECs of adult male and female mice (KO) using genetic procedures, and results were compared to animals wherein EC p62 is intact (CON). It was hypothesized that depleted p62 would induce arterial dysfunction. The efficacy and specificity of EC p62 depletion was evaluated in both groups by measuring p62 mRNA (qPCR) and protein (immunoblotting) expression in ECs and vascular smooth muscle. At tissue collection: (i) contraction to non-receptor (potassium chloride) and α-1 receptor (phenylephrine) mediated agonists, and relaxation to endothelium-dependent (acetylcholine) and independent (sodium nitroprusside) stimuli, were measured using isometric (mesenteric arteries) and isobaric (cerebral arteries) approaches; and (ii) intravascular signaling pathways (immunoblotting) important to vasorelaxation were assessed in both groups. Our preliminary findings found no significant differences in arterial function between groups. However, our data suggests that another cohort of mice may lead to statistical significance, and this is actively being conducted. While our results indicate EC specific p62 depletion does not impact arterial responsiveness, hypoxia or ischemia might unmask differences. Additionally, even though EC specific p62 depletion was inducible, other proteins with similar roles (e.g., NBR1) might have upregulated in a compensatory manner. All of these are topics for future study and projects are being designed to investigate them. While this study did not provide enough evidence to confirm the original hypothesis, it did provide several new questions that need answers, and this will direct the future research ambitions of this ongoing project.

Authors: Dallin Zollinger, Lauren Brooks. Mentors: Lauren Brooks. Insitution: Utah Valley University. Antibiotics are one of the most important inventions in recent history, saving countless lives. However, their use has also caused antibiotic resistance to increase in frequency among bacterial populations at an astonishing rate. This increase in frequency will influence our treatment of infections worldwide since previous treatments will no longer be effective, causing many deaths that would once have been preventable. Streptogramins are a class of antibiotics used to treat infections which bacteria have evolved resistance to. This study was focused on the vatA gene, which encodes resistance to streptogramins in Staphylococcus aureus. Samples were collected from various areas on the Utah Valley University campus, the DNA was extracted, and polymerase chain reaction (PCR) specific to the vatA gene was performed. Gel electrophoresis was then performed to visualize the amplicons generated from the PCR to evaluate the presence or absence of the vatA gene. Through this work, we have identified locations where this gene is found.

Authors: Sydney Holt, Mikaela Cowles. Mentors: Joe Jensen. Insitution: Utah Valley University. We are using Surface Brightness Fluctuations (SBF) to measure distances to giant elliptical galaxies using the Hubble Space Telescope (HST) and James Webb Space Telescope (JWST). These distance measurements will be used to better define the Hubble Constant, and hopefully help resolve the Hubble tension crisis in cosmology. Part of SBF requires a knowledge of the colors of the galaxies to be studied in order to account for variations in age and composition of the stars contained within. To this end, we are using PanSTARRS and DECam data to determine the optical g-z color (464 nm-900 nm) values for galaxies from the Virgo and Fornax clusters out to 300 million light years.

Authors: Isaac Settle, Easton Gray, Bonnie Anderson. Mentors: Bonnie Anderson. Insitution: Utah Valley University. Study of mode transitions with an artificially-blown clarinet Isaac Settle, Easton Gray, and Bonnie AndersenAbstractWhile playing the clarinet one might experience what is called a squeak or grunt note. A squeak note is a note that is in a higher register without any change of the keys being pressed. Similarly, a grunt note is a lower shifted note with the same circumstances. Both tend to be undesirable while playing, but often happen when learning how to play the clarinet. A similar occurrence of tonal differences can be controlled intentionally when advanced students enacting the technique called voicing to play the instrument in different registers. This research studied the physical parameters that cause squeak and grunt notes. An airtight box was built to hold the mouthpiece of the clarinet and attached to an air compressor. The effects on mode transitions between registers based on air pressure and where contact is applied on specific parts of the reed of the clarinet were studied. Additionally, a laser vibrometer was used to compare reed vibrations to that of the pitch of the clarinet. This data can help clarinet students as they learn the technique of voicing.

Authors: John Walton, Mason Astill, Jarrett Nelson. Mentors: Dustin Shipp. Insitution: Utah Valley University. Our research group is working to create a meta analysis of the variables that affect the efficiency of eddy current braking systems. We are making a track to accelerate marbles made from different non-ferromagnetic materials down an incline, through a photogate to get its initial velocity, then through a solenoid. We are testing several metals for the marbles and solenoids driven by alternating current with different frequencies. The marble after passing through the solenoid will have its new velocity recorded, allowing us to determine the deceleration experienced by the marble.

Authors: Sarah Adams, Bailey Bennion, Sara S Rocks. Mentors: Sara S Rocks. Insitution: Utah Valley University. The abundance of microplastics within Earth’s environment is of great concern to public health. Heavy toxic metals like lead and mercury concentrate on the surfaces of microplastics, and can readily enter the biosphere. This poses a risk to humans and other forms of life because of the large amount of microplastics that have been found in even the most remote locations on Earth. Not much has been studied about the origins and presence of microplastics in Utah snow. It would be beneficial to study microplastics in snow because, once the snow melts in the spring, it is a substantial source of water for Utah and communities in the American West. Preliminary data indicates increased concentrations of microplastics in snow samples that originated from ski resorts compared to snow samples in areas with little recreation. The current project further investigates the origins and quantity of microplastics in the snow at the Sundance ski resort. Snow samples from the Sundance ski resort and from a remote location with little human activity are compared. Additionally, the number of microplastics at Sundance between natural and artificial snow samples are compared to see if the plastic components within snowmaking machines generate a greater amount of microplastics in the snow. To investigate if snowboards and skis can release plastics, microplastics in fresh snow are quantified before and after snowboard contact. Because microplastics can also be shed from clothing, researchers also examined samples for fibers generated from winter garments. This project aims to shed light on the sources and number of microplastics in Utah snow, especially the effects of skiing and snowboarding.

Authors: Grant Cole, Vitaliy Walker, Daniel Adams, Nathan Twitchell, Melissa Lee, Jennifer Mortensen, Brittney Stockholm, Tatiana LeRoy. Mentors: Claudia Jorgensen. Insitution: Utah Valley University. When exposed to stressful situations, the human nervous system reacts with the fight or flight response activation. Physiological indicators of the activation of the fight or flight response include a change in body temperature, increased heart rate, blood pressure, and sweating (Sriram et.al, 2012). There is mounting evidence implicating stress as physiologically and psychologically harmful. For example, recent research has found a correlation between chronic exposure to stress and the development of mental illnesses such as anxiety and depression. Furthermore, chronic stress has the potential to change the anatomy of the brain along the hypothalamic pituitary adrenal axis (HPA), which is an important part of the neuroendocrine system, plays a role in the release of stress hormones, and helps regulate moods, emotions, and sexual behaviorisms (Ramirez, et.al., 2013). As college can be quite stressful, developing healthy coping mechanisms to deal with stress might positively influence the students’ academic performance and it might improve and maintain their psychological and physiological well-being (Skowronek, 2014). Various research studies have been conducted to reveal the therapeutic qualities of music. Music can effectively relieve nervousness, promote mental health, and positively affect students' psychological state (Chi, 2020). Current research on stress-related outcomes shows that music interventions play a significant role in stress reduction on physiological levels (de Witte et. al, 2020).  Preliminary results indicated that physiological responses (including heart rate, electrodermal skin response, and body temperature) to a mathematical calculation task were not impacted by the type of background noise (control, calm music, or upbeat music). Therefore, the current study is while being exposed to various music genre types (Kirschbaum et al., 1993). We hypothesize that calmer music might reduce stress response, while faster and more upbeat music might increase the stress response. The study will employ a between-subject measures design. After obtaining the baseline physiological measures when no music is played, the participant will either be exposed to two segments of relaxation/mediation music or two segments of fast-beat music. While exposed to different music conditions, the physiological response will be used to compare the impact of music genre (calm meditation music versus techno music) as well as practice (music segment 1 versus music segment 2) on the human stress response. Findings on the effects of music manipulation on the human nervous system can be used as additional tools for the management of stress.

Authors: Minh Nguyen, Eugene Leung. Mentors: Ming Yu. Insitution: Utah Valley University. A rapid, non-invasive method of disease detection method is vital for diagnosis or prevention. Many recent studies suggest that biomarker proteins that are associated with certain diseases were often found in urine samples of the patient. Quantum dots (QDs), a type of nanomaterial, have been investigated in previous studies, revealing their fast response in fluorescent properties when interacting with biomarker proteins. In this study, we examined the impact of QDs’ fluorescent properties in complex solutions by utilizing various concentrations of artificial urine samples. The results of this study provide insight into the possibility of using QDs as an alternative method of disease detection.

Authors: Vince Rossi, Spencer Brown. Mentors: Vince Rossi. Insitution: Utah Valley University. Current systems of generating 3D fluorescent images of samples require scanning of a sample or multiple exposures to acquire a series of images. Collecting a 3D fluorescent image in these fashions is timely, ranging from minutes to tens of minutes. This is not ideal for imaging live, dynamic samples that change as time progresses. The use of holographic techniques allows for the collection of a 3D image on the scale of milliseconds, therefore negating motion artifacts. Combining the speed of digital holography with fluorescent imaging will allow for identification of labeled biological components within a sample as well as the ability to view the sample in a 3D space thus offering greater detail and information on the sample. This process is called Holographically Informed Fluorescence Imaging (HIFI). Simulations have been run using MatLab in which a sample is imaged using Phase Shifting Digital Holography. The 3D reconstruction of the image is propagated using Huygens Convolution Method, and then all of the pixels of the image are brought into focus numerically. This image is then overlayed with the fluorescence image obtained. Each pixel of the fluorescent image is propagated to the depth of focus for each pixel within the 3D holographic image and a 3D fluorescent image is obtained. Simulations of this process have proved successful. A proof of principle experiment is currently underway as to prove the effectiveness of this fluorescent imaging method.

Authors: Sadie Jensen, Nellie Bowman, Nick Jeffery, Heather Wilson Ashworth. Mentors: Heather Ashworth. Insitution: Utah Valley University. The STEM career field has been found to be particularly unwelcoming to women and minorities resulting in attrition of these demographics in STEM degrees and careers.2 To address this problem, the National Science Foundation awarded Utah Valley University a grant to fund undergraduate research opportunities with faculty. This preliminary research project explores the effect of mentored undergraduate research opportunities on student retention and success in the UVU Biology Department. Each year for the last 4 years, 10 students were accepted each year into a research cohort. The following factors are known indicators of student success and retention in the STEM fields: self-efficacy1, effort control4, and teacher immediacy3. These factors were qualitatively measured with reflection questions. Students were surveyed at the end of each academic semester. There are two completed cohorts and data is still being collected on the remaining two cohorts. A coding scheme was developed, and reflection questions were coded to consensus. The Pearson's coefficient was greater than 0.76. The preliminary coding results show themes of positive or very positive effects of the research experience on self-efficacy, effort control, and teacher immediacy. The greatest gains were observed in self-efficacy and teacher immediacy. This preliminary data supports the role of undergraduate research in retaining women and minorities in STEM majors. Sources: Kalender, Z. Y., Marshman, E., Schunn, C. D., Nokes-Malach, T. J., & Singh, C. (2020). Damage caused by women’s lower self-efficacy on physics learning. Physical Review Physics Education Research, 16(1). https://doi.org/10.1103/physrevphyseducres.16.010118 White, J. L., & Massiha, G. H. (2016). The retention of women in Science, Technology, engineering, and Mathematics: A framework for persistence. International Journal of Evaluation and Research in Education (IJERE), 5(1), 1. https://doi.org/10.11591/ijere.v5i1.4515 Micari, M., & Pazos, P. (2012). Connecting to the professor: Impact of the student–faculty relationship in a highly challenging course. College Teaching, 60(2), 41-47. Pintrich, P. R., & De Groot, E. V. (1990). Motivational and self-regulated learning components of classroom academic performance. Journal of educational psychology, 82(1), 33.

Authors: Allison Perkins, Aubrey Hawks, Talia Karasov. Mentors: Talia Karasov. Insitution: University of Utah. Over the past two decades, studies have documented a 20% decline in Quaking Aspen (Populus tremuloides) populations in western North America (Worrall et al., 2015; Stanke et al., 2021). This phenomenon has been fittingly characterized Sudden Aspen Decline (SAD), and is an increasingly pressing issue as the role of aspen as an ecologically irreplaceable keystone species impacts the health of the surrounding forests (Singer et al., 2019). SAD has been attributed to the interplay of climate change-driven drought and other biotic and abiotic factors that are less well characterized (Anderegg et al., 2013a). One potential contributor to SAD is biotic pests and pathogens (Marchetti et al., 2011; Anderegg et al., 2013a; Worrall et al., 2015). My study system includes both natural populations of aspen representing a precipitation gradient and a controlled garden experiment. The field experiments span five distinct sites across Utah & Colorado, selected and montintered by the Anderegg lab of the University of Utah. The experimental garden contains approximately 300 tree saplings subjected to various levels of drought stress, managed by the Anderegg lab on the University of Utah campus. Through the integration of both controlled and natural experiments, my research aims to comprehensively evaluate the impact of drought exposure on pathogen abundance and chemical defenses in aspen trees.Over the past two decades, studies have documented a 20% decline in Quaking Aspen (Populus tremuloides) populations in western North America (Worrall et al., 2015; Stanke et al., 2021). This phenomenon has been fittingly characterized Sudden Aspen Decline (SAD), and is an increasingly pressing issue as the role of aspen as an ecologically irreplaceable keystone species impacts the health of the surrounding forests (Singer et al., 2019). SAD has been attributed to the interplay of climate change-driven drought and other biotic and abiotic factors that are less well characterized (Anderegg et al., 2013a). One potential contributor to SAD is biotic pests and pathogens (Marchetti et al., 2011; Anderegg et al., 2013a; Worrall et al., 2015). Recent investigations have indicated a link between SAD and specific microbial diseases, suggesting that the increasing frequency and severity of droughts due to climate change might make aspen more vulnerable to certain pathogens, even though many of the most common pathogens of aspen in general require more abundant water (Aung et al., 2018). For example, the foliar Melamspora fungal pathogens require abundant water and are not frequently observed in drought stressed trees.On the other hand, Cytospora, which causes a devastating canker disease in aspen trunks, occurs at higher frequency in plots of trees suffering damage from drought (Guyon, 1996). Lin et al. (2023) shows changes to phyllospheric microbiome in aspen during drought, but far less is known about the leaves specifically. Could drought lead to an altered microbiome in aspen leaves? If different or possibly opportunistic pathogens are better able to colonize the leaf tissue under drought stress, this may be the case.It’s known that plants with a reduced diversity of microbiomes are more susceptible to pathogens (Zheng et al., 2020), but the there is little understanding how drought may reduce microbial diversity in aspen. Aspen have two main groups of chemical defenses (SPGs and CTs) that occur in relatively high levels in the leaf (Lindroth et al., 2023). It is well established that these defend against insect herbivores and that they come with a trade-off for plant growth (Marchetti et al, 2011). There is some observational evidence that these secondary compounds have an effect on pathogens (Jacoby et al., 2021), but there are few controlled studies on this idea. Additionally, Metlen et al. 2009 describes how trees in North America produce higher rates of these metabolites under wetter conditions, attributed to possible UV protection. However, the impact of additional environmental changes have of on the abundance of these compounds is understudied. Aspen are an ideal system to study forest disease and drought because the genus’ (Populus) genomes are easily sequenced and relatively tractable, aspen generally exhibit rapid vegetative growth, their defensive chemistry is relatively well known, and most importantly, they grow in cloned groves that reduce the genetic variation in experiments (Lindroth et al., 2023, Luquez et al., 2007).

Authors: Braxton Bird. Mentors: Sarah Franklin. Insitution: University of Utah. Heart disease ends the lives of nearly 700,000 people each year and has been the leading cause of death in the United States since 1950. Around this time researchers discovered that some modifications involving our genetic code could be altered to affect gene expression but leaves the DNA intact, which was later termed epigenetics. Today we’ve discovered that these epigenetic modifications, including post translational modifications (PTMS), regulate genes linked to cardiovascular disease. We recently examined the histone lysine methyltransferase SETD7, which is most prominently known for its ability to methylate histone H3K4. SETD7’s expression is upregulated in multiple types of heart disease in both humans and mice and is essential for cardiomyocyte differentiation in embryonic development. In addition to its ability to methylated H3K4, SETD7 has been shown to methylate 8 other histone residues. To further characterize the histone residues methylated by SETD7, we carried out an unbiased analysis of lysine residues methylated by SETD7 using an in vitro methyltransferase assay coupled with tandem mass spectrometry. We hypothesized that SETD7 may modify additional sites than those that have previously been identified. Our analysis determined that SETD7 monomethylates two novel residues on histone H3: lysine 36 (K36) and lysine 122 (K122). These sites of modification were also confirmed by western blotting for site specific antibodies to these methylation marks. Although our understanding of both these residues is limited, we do know that K36 methylation is linked to DNA replication and genomic stability while K122 methylation is downregulated in drug-resistant MCF-7/ADR cancer cells. These two novel methylation sites suggest that this lysine methyltransferase plays a more complex role in regulating epigenetic modifications and gene expression than previously recognized. Although the identification of this new enzymatic activity for SETD7 is important for understanding the dynamic function of methyltransferases, additional studies will be necessary to fully elucidate the role of SETD7 in cardiac physiology and gene regulation.

Authors: America Cox, Kendra Autumn , Bryn Dentinger. Mentors: Bryn Dentinger. Insitution: University of Utah. Neotropical ants of the Attini tribe evolved the innate ability to farm fungi. Agaricomycetidae contains two clades that are cultivated by ants: the lepiotaceous and pterulaceous cultivars. However, there are free-living relatives phylogenetically distributed throughout each cultivar clade. Comparison between the free-living relatives and attine system cultivars may identify the evolutionary differences caused by, or initially enabling, agricultural symbiosis. Our research compares free-living relatives and cultivars through genomic and metabolic analysis. Attines undertake significant cultivar maintenance through the regulation of pathogenic contamination of their fungal “gardens” as well as the provision of specific growth substrates, including insect frass. Noting the apparently specialized substrates cultivars receive from the ants, we conducted a pilot test for a growth media preference between a lepiotaceous cultivar, a pterulaceous cultivar, and a free-living pterulaceous relative. We placed the fungi on regular PDY media and PDY media infused with caterpillar frass, and the cultivars either changed structure or had improved growth on the frass-infused media. Following the pilot test, we will run metabolic assays on the cultivars and free-living relatives on different media types. This may indicate a media preference which gives further insight to the attine-fungal symbiotic relationship opposed to the fungal free-living relatives revealing pieces of the fungi’s evolutionary history. We performed DNA extraction, PCR testing, Sanger sequencing of the ITS region, and then whole genome sequencing on the cultivars and their free-living relatives. Sanger sequencing allowed us to build phylogenetic trees to examine the relationship between the free-living fungi and cultivars. The whole genome sequencing allowed us to use antiSMASH software to generate predicted secondary metabolite clusters in a fungi species that “escaped” cultivation, a cultivar, and a free-living relative. This preliminary data suggests a diversification of fungal secondary metabolites occurs after attine domestication. By looking at fungal metabolic and genomic data, we hope to gain insight into the fungi’s evolutionary history and agricultural symbiosis.