Fine Arts
Lighting for Different Land Uses: A Case Study in Cedar City
Authors: Sebastian Partida. Mentors: Jamie Spinney. Insitution: Southern Utah University. Lighting levels have an important role in influencing the ambiance and functionality of various land uses. To achieve specified goals while optimizing energy efficiency and sustainability, lighting intensity must be balanced. The purpose of this study was to compare light intensity for different land uses (e.g., residential, commercial, industrial, and recreational). This study measured light intensity (i.e., brightness) for a random sample of different land uses using a digital light meter within Cedar City, UT. GPS coordinates were also acquired in order to map the brightness data. As expected, the results revealed that residential land uses were the darkest, whereas commercial and industrial land uses were the brightest. The findings indicate communities have to maintain a balance between creating a warm and pleasant atmosphere and ensuring appropriate illumination for safety and security purposes. Energy-efficient LED fixtures and smart lighting systems, for example, can provide adjustable and programmable illumination settings that meet both aesthetic and safety requirements.
Evaluating the role of LRRC1 in melanoma growth and metastasis
Authors: Kylee Fernandez. Mentors: Gennie L Parkman. Insitution: University of Utah. Evaluating the role of LRRC1 in melanoma growth and metastasisThe development of brain metastases is a critical problem in Utah, regardless of the novel therapies created for melanoma. The melanoma mortality rates in Utah are 31% higher than the national average and the melanoma incidences are 80% higher. To lower these incidences, our research is aimed at investigating targets in melanoma that play a crucial role in brain metastasis and can be identified as therapeutic interventions.LRRC1 is a gene known as Leucine Rich Repeat Containing 1. LRRC1 is involved in early-stage adipocytic differentiation. Altered gene and protein expression of LRRC1 is observed in melanoma potentially due to the vital role LRRC1 plays in adipose stem cells that differentiate by adopting the phenotypic, biochemical, and functional properties of more terminally differentiated cells. To evaluate the effect of LRRC1 on melanoma growth and metastasis, we are using an established autochthonous in vitro and in vivo model of melanoma based on the RCAS/TVA avian retroviral system. The results of this study will have tremendous potential to contribute to the understanding of the genetics of this heterogeneous disease.
Bx42 is Required for Brain Development and is Linked to Microcephaly
Authors: Adriana Bibo, Nicole Losurdo, Nichole Link. Mentors: Nichole Link, Cindy Greaves. Insitution: University of Utah. Microcephaly is a neurodevelopmental disorder characterized by occipital frontal circumference (OFC) more than two standard deviations below the mean. It can be accompanied by comorbidities including intellectual disabilities, seizures, and other developmental phenotypes. Microcephaly is attributed to malnutrition and exposure to toxins or infection during pregnancy, but genetic mutations are also a leading cause. To investigate the genetic mechanisms behind microcephaly, our lab collaborates with a physician who has completed whole exome sequencing for two hundred microcephaly patients. He identified potentially pathogenic human variants, which we are studying using Drosophila melanogaster as our model organism. We screened for loss of function phenotypes through RNAi knockdown of fly orthologs to determine if these genes are necessary for brain development. In our primary screen, we found that RNAi knockdown of Bx42 in neural stem cells or post-mitotic neurons causes significantly reduced brain lobe volume. In our current study, we are assessing potential causes for reduced brain lobe volume. We seek to determine if RNAi knockdown of Bx42 in neural stem cells or neurons affects cell number, proliferation, or death in developing brains.
Social Effects of Bed Nucleus of the Stria Terminalis Corticotropin-releasing Hormone and Tachykinin 2 Neuronal Activation Using Optogenetic Stimulation
Authors: Nicholas Poll. Mentors: Moriel Zelikowsky. Insitution: University of Utah. Social interaction is one of the chief drivers of evolution and natural selection. Animal interactions shape the ecological world and have shaped the world’s evolutionary timeline. Of the many types of animal interactions, one of the most influential to behavior and development is aggression. Many animals' aggressive behaviors and mechanisms are so distinct that their origins can be traced back to specific selective pressures. These pressures range from reproductive competition to predator-prey interactions and have apparent effects on many species’ developments. There are many stressors that can increase aggressive tendencies found in mammals, yet it’s clear that the neural bases that connect these stressors and their behavioral responses are understudied. The targeted brain region in this project is the bed nucleus of the stria terminalis (BNST). The BNST is a neurochemically diverse node of the extended amygdala that has been implicated in mediating stress and sensory information. It anatomically acts as a relay station between a number of different structures including the hypothalamus and amygdala. The BNST has been implicated to have a role in stressor-induced aggressive behaviors; however, the neural mechanisms that connect this region and these behaviors are relatively understudied. Corticotropin-releasing Hormone (CRH) in mammals displays upregulation following the introduction of a stressor and has been found to mediate aggression and anxiety. Similarly, tachykinin-expressing neurons have been shown to have a role in mediating aggression in mammals. While CRH and tachykinin’s link to anxiety and aggression is well supported, it is not clear to what extent it has on BNST. This project aims to provide data to support the role that CRH and Tachykinin 2 (Tac2) in the BNST have in mediating aggression in mice through optogenetic stimulation.
In the Depths of the Understory: Costa Rican Associations Between Ants and Coccoidea Unraveled using NGS DNA Barcoding
Authors: Sylvia Lee, Ainsley Parkins, Rodolfo S Probst. Mentors: Rodolfo S Probst. Insitution: University of Utah. Mutualisms between ants and plants are ubiquitous interactions and have been evolving for over 100 million years. Studies have been done on systems wherein the plants provide nutritional resources for ants, but the dynamics of mutualistic interactions in environments where host plants do not provide obvious nutritional rewards continue to remain elusive, raising questions about their co-evolutionary histories. In these circumstances, associated fauna, such as mealybugs and soft scales (Hemiptera: Coccoidea: Pseudococcidae and Coccidae), serve as intermediaries of the association, providing honeydew (enhanced plant sap) as a main food source to their host ants. However, the identity of the insects involved in these associations and their co-evolutionary history with ants is poorly understood. By focusing on samples collected in Costa Rica from 33 colonies of the Neotropical plant-ant genus Myrmelachista (Formicinae: Myrmelachistini), we sequenced DNA barcodes (cytochrome oxidase subunit 1 [CO1]) using Oxford Nanopore Technology and employed Next-Generation Sequencing (NGS) for both the Myrmelachista ants and their associated fauna. We aimed to identify their taxonomy, phylogenetic relationships and identify unique interactions between ants and their associated fauna. We recovered DNA barcodes for eleven Myrmelachista species and seven putative species of mealybugs (n=5) and soft scales (n=2). For the seven putative species of Coccoidea, our DNA barcodes, when compared to available sequences from the NCBI database, recovered an average of 85% match similarity. The closest matches associated our putative species to Old World Coccoidea taxa, reinforcing the need for taxonomic studies of this superfamily for the Neotropical region. Each putative species of soft scales was found uniquely associated with two Myrmelachista species (M. flavoguarea and M. cooperi). For all eleven Myrmelachista species, five were associated with two putative mealybug species. One putative mealybug species was associated with five different Myrmelachista species; one was uniquely associated with M. nigrocotea. Same associations were found between the associated fauna of M. flavocotea and M. lauropacifica; M. haberi and M. RSP027; M. plebecula, M. meganaranja, and M. osa, respectively. These results suggest that there may be unique associations between some Myrmelachista species and their Coccoidea associates, unconstrained by the ant phylogeny. Our findings also provide insights on Coccoidea taxonomy, emphasizing NGS DNA barcoding as a tool to illuminate how ant-plant associations might have evolved.
Genetic Mutations in the Genomes of Mother-Offspring Pairs Shows Link Towards Nausea and Vomiting in pregnancy
Authors: Dallin Oyler, Ana Alfaro, Kailee Myxter, Ben Brooks, Amanda Brooks. Mentors: Jessica Pullan. Insitution: Southern Utah University. Nausea and vomiting are common complications that occur in 70% of pregnancies. Hyperemesis Gravidarum (HG) is the most severe form of these symptoms and is estimated to be prevalent in 0.3-2% of pregnancies. Due to the fetal and maternal morbidity associated with HG, identifying the cause and treatment options for these women is a critical task in obstetrics. Research regarding the etiology of HG has been fairly recent and is still ongoing, however, evidence had directed to a positive correlation between increased levels of the serum protein GDF15 and HG symptoms. We hypothesize that polymorphisms in both maternal and fetal DNA plays a role in the upregulated GDF-15 seen in mothers experiencing symptoms of HG during pregnancy. The DNA of 2 mothers and their corresponding children were sequenced and analyzed. The DNA was obtained through buccal swabs from the epithelial cells of the inner cheek, and then purified and ran through PCR. We employed 3 distinct primers that correspond to mutations in the genome that account for the elevated levels of circulating GDF-15 in the mother. As of current, the mother-daughter DNA is still under analysis for single-nucleotide polymorphisms, however recently published literature has suggested results similar to our hypothesis. M. Fejzo et al. shows that upregulated serum GDF-15 is primarily of fetal origin, and that maternal sensitivity to GDF15 increases the risk for developing HG. Additionally, the DNA coding variant GDF15 C211G was shown to elevate the risk of HG
Reconstructing seasonality in African bovids using dental microwear
Authors: Auriana Dunn. Mentors: Kathryn Sokolowski. Insitution: University of Utah. Reconstructing the diet of extinct animals can help us understand how living species are suited to their environments, which can inform paleoecological and zooarchaeological research as well as modern species conservation. Seasonal variation in animals’ diets can further be used to reconstruct seasonality patterns in the past and understand how animals may vary their diet under future climate change. However, determining the diet of extinct species is challenging, and detecting seasonal changes in diet is even more so. Dental microwear texture analysis (DMTA) can be a tool to solve this problem. DMTA analyzes dental microwear by measuring the pits and scratches on teeth to determine what an animal consumed in the days and weeks before it died. This project examines the DMTA of modern African Bovidae species to determine if seasonal dietary changes can be observed. If a difference can be found in the dental microwear of specimens that died during different seasons, seasonal changes in diet can be inferred. Here, I gathered dental microwear data from 407 specimens across five modern studies and matched the data to the museum specimens’ date of death to determine whether it died in a wet or dry season. The analysis found that four of the Bovidae species examined in our study showed evidence of seasonal diet shifts in their DMTA data. Dental microwear can be useful in determining seasonal diets and season of death for specimens. This method can be applied to extinct Bovidae species to understand ancient ecosystems and the evolution of Bovidae dietary strategies.
Murine Model of Hepatitis Delta Virus-Associated Salivary Gland Impairment
Authors: Alexander Romano, Matthew Hesterman, Rachel Hill, Melodie Weller. Mentors: Melodie Weller. Insitution: University of Utah. Sjogren’s Disease (SjD) is an autoimmune disease diagnosed by symptoms of reduced tear and saliva excretion, accumulation of lymphocyte foci in the salivary glands, and the occurrence of antibodies against Ro (SSA), La (SSB), and nuclear proteins. The cause of SjD is unknown, though previous studies have detected the sequence and antigens of Hepatitis Delta Virus (HDV) in the minor salivary gland acinar, ductal, and adipose cells of patients with SjD; without the typical presence of a Hepatitis B coinfection. In this study, murine models were transduced with Adeno-associated virus containing expression cassettes for Luciferase (control), small, large, or a combination of both small and large HDV antigens to evaluate the impact of HDV antigen expression on salivary gland function and SjD autoimmune disease development. After a ten- or four-month period, the models were analyzed. Findings included a significant increase of inflammation for samples expressing both small and large HDV antigens, a significant increase of anti-SSA(La) antibodies in samples expressing the short HDV antigen, and a correlation between increased overall inflammation and decreased overall saliva flow. Performing qPCR methods verified the amount of HDV in the submandibular glands and Illumina sequencing portrayed an increase in glycolysis and beta oxidation metabolism in models with detectible HDV sequence. The models showed significantly increased IgM expression in the HDV exposed murine models, without significant change of other antibodies. Future plans include an ELISA diagnostic assay to verify the antibody levels and further sequencing analysis. With this information we can build a better picture of the direct mechanisms of HDV-mediated changes in salivary gland dysfunction and determine the extent that HDV can inducing systemic SjD symptoms.
Using Past Ecosystems to Understand Modern Climate Change: A Case Study from Utah’s House Mountain Range
Authors: Jane Damstedt, Kasey Cole. Mentors: Kasey Cole. Insitution: University of Utah. In this project, we are analyzing faunal remains recovered from Utah’s House Mountain Range in the Tubafore cave system to create a paleoecological baseline of life in that location over the past 1000 years. All animals have relatively consistent ecological tolerances and preferences, so comparing the data from the information obtained in this study with contemporary accounts of mammalian ecology in the same area will be useful in evaluating the role of anthropogenic climate change in mammalian ecosystems. In this project, I am identifying the animal remains to the lowest level of taxonomic classification possible to determine what kinds of species richness and environment were present at the time in which the animal was alive. Creating an accurate record of the animals found in this cave assemblage is critical because very little is known about the species richness of the Great Basin area. This data will then be stored and compared to modern zoological data, which came from online databases Actos and Vertnet. The information gathered from this project can be used to inform conservation and sustainability efforts aiming at preserving biodiversity in Central Utah’s Mountain Ranges.
Going Green in a General Chemistry Teaching Lab: Assessment of Learning Outcomes
Authors: Cooper Bell-Hunley, Ming Yu, Emily Heider. Mentors: Emily Heider. Insitution: Utah Valley University. Green chemistry involves the redesign of chemical processes and products to reduce hazards to the environment and human health. Reconsidering the teaching curriculum with a view to embrace green chemistry can reduce costs in waste disposal and has the potential to improve accessibility. This research involves the redesign of qualitative analysis general chemistry experiment to eliminate the use of teratogenic mercury (Hg) and neurotoxic lead (Pb). Making this change creates a more inclusive laboratory since expectant mothers should not be exposed to mercury and therefore some students did not feel safe completing the previous version of the experiment. With any change in curriculum, it is important to consider the impact on learning outcomes that students can achieve. This research details the assessment of learning outcomes with a quasi-experimental design. Instructors in multiple general chemistry labs selected the qualitative analysis experiment (with or without Hg and Pb) and learning outcomes achieved were assessed following the experiment.
Nonequilibrium Thermodynamics of Voltage-Gated Ion Channels
Authors: Ada Cottam Allen, Mikhael T Semaan. Mentors: Mikhael T Semaan. Insitution: University of Utah. Regularly buoyed from thermodynamic equilibrium by complex and fluctuating environments, living systems must continually respond and adapt to external stimuli. Recent tools from stochastic thermodynamics place concrete energetic and entropic bounds on these processes, for systems arbitrarily far from equilibrium both in and out of steady-state conditions, and thus reveal mechanisms by which these systems absorb and dissipate energy to function. Here, we leverage these tools to simulate and calculate the thermodynamic signatures of several models of sodium and potassium ion channels---channels crucial to propagating action potentials in mammalian neurons---as driven by biologically-realistic action potential spiking protocols. Thus uncovering their detailed energetic responses to such complex environmental conditions, these results enable detailed comparison between the thermodynamic functionality of each channel type with driving one would expect in-vivo. When applied to competing models of the same channel, these tools also provide additional criteria for model selection; to this end, we close by calculating expected ionic currents, directly facilitating comparison with dynamically-driven patch-clamp experiments.
Impact of Inducible Depletion of Sequestosome-1 (p62) From Endothelial Cells On Intrinsic Arterial Stiffness
Authors: G. Colton Gardner, Lynasi Gapelu, Sohom Mookherjee, Sihem Boudina, J. David Symons. Mentors: J. David Symons. Insitution: University of Utah. With the prevalence of cardiovascular disease increasing world wide and its precise mechanisms remaining elusive, the need for novel treatments is urgent. Autophagy is a conserved process whereby dysregulated intracellular proteins are escorted to the lysosome for recycling. Emerging evidence indicates that autophagy is important in maintaining endothelial cell (EC) proteostasis and function. My laboratory showed that autophagy repression in adult mice ECs produces arterial dysfunction, and indexes of autophagy are repressed in ECs of older mice and humans which display concurrent EC dysfunction. Sequestosome 1 (p62) tethers proteins and enables their entry into the autophagic pathway. The contribution from EC p62 to cardiovascular and metabolic function is unknown and experiments are needed to investigate this. Thus, p62 will be depleted from ECs of adult male and female mice (p62iEC-/-) via tamoxifen regimen, and results will be compared to animals wherein EC p62 is intact (CON). The efficacy and specificity of EC p62 depletion will be evaluated in ECs and vascular smooth muscle by qPCR and immunoblotting. Three adjacent 1 mm thoracic aorta sections will be obtained 14 days after the tamoxifen regimen. The central aorta section will be used to measure intrinsic mechanical stiffness by wire myography, with the other two used to find lumen diameter and intimal medial thickness. These measurements will help create Stress vs. Strain curves and the Elastic Modulus, which will be compared between the groups to show the differences caused by p62 depletion. We expect p62 depletion from ECs to increase intrinsic aortic stiffness.
A Metagenomic Analysis the Microbial Composition of Apis mellifera Pollen Preserves throughout the Foraging Season
Authors: Sydney Larsen. Mentors: Joshua Steffen. Insitution: University of Utah. Pollen preserves are crucial to maintaining Apis mellifera’s hive health. This pollen provides bees with a source of protein, amino acids, lipids, and other nutrients that are vital for larvae development (Dharampal et. Al, 2019). In addition to providing these essential nutrients, bee collected pollen also provides the hive with an excellent source of external pollen-borne microorganisms. These microbes provide a variety of benefits including serving as a major dietary requirement for larvae, assisting in biochemical processes such as bee bread fermentation, and assisting in bee resistance to pathogens (Gilliam, 1997). Despite the importance that these organisms play on bee health, there is currently little research on how the microbial composition of bee foraged pollen changes throughout the foraging season in response to abiotic and biotic factors. Rapid advancements in DNA metabarcoding approaches and DNA sequencing technologies have made the assessment of hive level foraging patterns easily accessible. We attempt to leverage the benefits of these new approaches to characterize how microbial composition in pollen provisions changes in relation to shifts in foraging preferences. Here we describe general patterns plant foraging behaviors for two hives across a single foraging season. In addition, we describe the variability of microbial diversity associated with pollen across this same time frame. Our initial description plant and microbial communities present in pollen provisions suggest that pollinator foraging preferences play a significant role in pollen associated microbial communities. This will allow for further research into how the presence of specific beneficial microbes is affected by changes in climate, floral availability, pathogen presence, etc..ReferencesDharampal, P. S., Carlson, C., Currie, C. R., & Steffan, S. A. (2019). Pollen-borne microbes shape bee fitness. Proceedings of the Royal Society B, 286(1904), 20182894.Gilliam, M. (1997). Identification and roles of non-pathogenic microflora associated with honey bees. FEMS Microbiology Letters, 155(1), 1–10. https://doi.org/10.1016/s0378-1097(97)00337-6
A Glance into the Origin of Life through the Lens of the DNA Repair Enzyme MutY from the Lost City Metagenomes
Authors: My Nha Quyen Tran, Cindy Greaves. Mentors: Martin Horvath, Cindy Greaves. Insitution: University of Utah. I have been studying the DNA repair enzyme MutY found at the Lost City thermal field 3000 feet deep in the Atlantic Ocean with conditions similar to those that may have shaped the origin of life. With no sunlight and very little oxygen, life at the Lost City is driven by chemical transformations in rocks at extremely high levels of pH and temperature. DNA in all organisms strictly follows base pairing rules in which A pairs with T and G pairs with C. We all think of oxygen as beneficial, but it can cause damage to DNA. Oxidized guanine (OG) violates the base pairing rules and pairs with A. This causes mutations, which can lead to changes in the genetic information. The mutagenic potential of OG was a challenge that had to be solved early in the emergence of DNA-encoded life. The enzyme MutY functions in suppressing mutations and therefore protects us from developing cancer by finding and removing A from OG:A mispairs. In my effort to replicate conditions at the Lost City so as to explore how the DNA repair enzyme MutY evolved to function in this strange environment I need to purify the enzyme. I used DNA cloning to attach the MutY-encoding genes to a soluble bacterial protein called GST that hopefully will make it easier to purify the MutY proteins. Encouragingly, these fusion proteins expressed well in bacteria and appeared to be soluble. I am now exploring different conditions looking for optimal enzyme activity, which I predict to be different from what familiar bacteria can tolerate. Basic science research on how DNA repair enzymes adapted to the strange environment at the Lost City may inform future translational applications to treat and cure cancer and thus ensure a healthy society in Utah.
Synthesis and testing of hydrophobicly-modified pyridyl-aza crown ethers as chelators of rare earth elements.
Authors: William Lee, Rishab Nayak, Cecilia Knapp, Camila Soderholm. Mentors: Ryan Stolley. Insitution: University of Utah. Recent publications have shown pyridine carboxylate functionalized aza-crown ethers coordinate strongly to a number of rare earth elements. Considering their strong coordination, we have modified these crown-ethers with a number of hydrophobic moieties to allow for adsorption to non-polar substrates to enable packed bed isolation of these elements to address the broad use and limited availability of these elements.
Information Anatomy Over Partition Space
Authors: Nathan Jackson, Ryan James, James Crutchfield. Mentors: Mikhael Semaan. Insitution: University of Utah. Symbolic dynamics allows for modeling---and designing for---the effects of imperfectly measuring a time series of data, by partitioning into a finite number of possibilities. The resulting time series of discrete symbols is then made especially amenable to information-theoretic methods for understanding its temporal structure and correlation. In particular, as long as the partitioning scheme is generating, the resulting estimations for entropy rate---the rate at which the process creates information---converge to a measure of chaos in the underlying system, a dynamical invariant.However, the entropy rate is not sensitive to what kind of generating partition: colloquially, an instrument must be at least accurate enough, but can be more fine-grained as desired. In contrast, its breakdown into a piece which affects future measurements (“bound”) and a piece which does not (“ephemeral”) depends quite dramatically on the choice of generating partition. We ask, then: is there a canonical partitioning scheme for which the full suite of information measures relate to dynamical invariants?To tackle this, we simulate the well-known tent and logistic maps, sweeping over all two-boundary partitions, calculating the full information anatomies for each. We find that ephemeral and bound information are extremized by the single-boundary coarsest generating partition, suggesting a canonical role for the simplest “good enough” instrument. The remaining multivariate measures, meanwhile, reveal hitherto-unseen structure in the process of imperfect measurement.
The use of ecological niche modeling to characterize the microhabitat of oak hybrids (Quercus undulata) in Utah
Authors: Cate Bateman, Carson Veazie, . Sydney Sandall. Mentors: Matt Ogburn. Insitution: Southern Utah University. Western Utah is home to two distinct species of oak, Gambel oak (Quercus gambelii) and Dixie live oak (Quercus turbinella), which can hybridize to form an intermediate species that has been named Quercus undulata. In spite of previous research on these shrubs, we are still unsure of how the hybrid has survived in small isolated locations well outside of the range of one parent species (Dixie live oak, which is restricted in Utah to Washington, Kane, and San Juan Counties). Our primary objective was to perform an ecological niche modeling assessment of Q. undulata within the Three Peaks Recreation Area. Our approach included collecting plot-level data to determine abiotic and biotic niche factors correlating with the presence/absence of Q. undulata, including slope, aspect, soil type, soil chemistry, vegetation cover, and other associated woody plant species. Our results have shown that Q. undulata populations have higher concentrations in south-facing slopes, potentially reflecting the climatic preferences of its Dixie live oak parent. Results also showed that both focal species, Q. undulata and Q. gambelii, prefer areas with shallow soil and rocky outcrops. We plan to continue data collection and our analysis of soil type and chemistry. These results will help us to better understand the evolutionary history and ecological requirements of this fascinating native Utah plant.
Isolation on Plant Growth promoting bacteria from the hybrid buffaloberry Shepherdia x utahensis ‘Torrey'
Authors: Ty Wilson. Mentors: Amita Kaundal. Insitution: Utah State University. Climate-induced environmental stresses and the increased use of fertilizers to meet the increasing demand for food significantly threaten food production and soil health. In natural environments, microbes are vital to the growth and development of plants. Their presence in the soil allows for enormous flexibility and nutritional availability for plants, particularly those living in harsh conditions. A plant’s microbiome, especially the underground, plays a significant role in its growth and development and mitigates environmental stresses. Idaho and Utah are particularly challenging environments due to extreme temperatures and arid soil. However, despite these difficult environmental factors, the native plants of Utah and Idaho can thrive. Here, we are exploring the rhizosphere microbiome of Shepherdia x utahensis ‘Torrey,’ a hybrid of two highly drought-tolerant buffaloberry species, S. argentea and S. rotundifolia native to the Intermountain West region of the US to isolate plant growth promoting bacteria. In this study, we isolated drought-resistant plant growth-promoting microbes from the rhizosphere of hybrid buffaloberry. Root samples of hybrid buffaloberry were collected from three locations in Utah: the USU campus, Greenville Farm USU, and the USU botanical garden, Kaysville. The rhizosphere was removed from these root samples and pooled together. The bacteria were isolated on five different bacterial growth media twice. The isolated microbes were then purified using the streak plate method. Forty-six unique isolates were selected based on morphological characteristics and tested for four plant growth-promoting traits. Fourteen bacteria could show the ability to fix nitrogen fix, 18 produced a siderophore, 17 could solubilize phosphate, and 11 produced catalases. IAA production, ACC deaminase, and protease activity assays are in progress. All isolates will be identified by 16S rRNA Sanger sequencing.
Insane in the Membrane: Amino acid sequence evolution and conservation in vital protein complex in gram-negative bacteria
Authors: Alexander Everett, David Bean, Jeremy W Bakelar, Randy L Klabacka. Mentors: Randy L Klabacka. Insitution: Utah Tech University. β-barrel assembly machinery (BAM) is a protein complex vital to cell survival in gram-negative bacteria that functions to insert proteins into the cell’s outer membrane. BamA, a protein composed of a membrane-bound beta barrel and several POTRA domains that protrude into the cytosol, is the primary subunit within BAM. It is suggested that BamA is conserved due to its widespread presence across the bacterial tree of life. Here we quantify the conservancy of BamA in 142 species of gram-negative bacteria from across the bacterial tree of life, with a focus on 120 species belonging to the order Enterobacterales. In addition, we compared the conservancy of BamA with other proteins with high and moderate levels of conservation in Enterobacterales. Lastly, we discuss regions of BamA that are of high conservation in the context of their potential as antibiotic targets.
Eolian Sediment as a Potential Soil Parent Material in Cache Valley
Authors: Kylie Hansen. Mentors: Janis Boettinger. Insitution: Utah State University. Cache Valley, Utah, has unique soils due to environmental events such as Pleistocene pluvial Lake Bonneville. This lake extended about 20,000 square miles in Northern Utah, and its depletion left numerous shorelines, the most relevant being the Bonneville and Provo shorelines. These various water levels influence the geological formations in Cache Valley and the different characteristics of soil formation. An important soil-forming factor used to characterize varying soils is the parent material. Parent material is the original sediments the soil formed on and can be various things such as sediments deposited by gravity, streams, lake sediments, or wind. These different materials depend on the location and alter the soil properties. A dominant soil parent material in the Lake Bonneville area is sediment deposited by this lake. This type of parent material is identifiable by coarser-sized sediments, such as cobbles, gravel, and sand. This pattern is evident on terraces in Cache Valley’s lower soil layers, but the near-surface layers have fewer rock fragments and more fine sand, silt, and clay. This distinct difference in particle size leads to the question of what is the parent material source in the surface horizons of soil formed in Lake Bonneville deposits in Cache Valley. There is not much literature on this question and little documentation compares the soil profiles for different Lake Bonneville terrace levels (Bonneville shoreline compared to Provo shoreline). We hypothesize that the different particle sizes of parent material in the surface of soils formed dominantly in Lake Bonneville deposits is eolian sediment transported and deposited by wind. To determine this, we will sample soils across Cache Valley and analyze the fine and very fine sand sediments in plane-polarized and cross-polarized light. This process will identify various minerals within each soil horizon, showing possible differences in the upper and lower horizons. The minerals present in each soil will indicate the parent materials, allowing us to understand what is causing the different textured horizons in these soils and where the sediment originated from.
fr10 Evolution
Authors: Reagan McKee, Vicente Fernández Lara, Jeremy Bakelar, Randy Klabacka, Dustin Kolste. Mentors: Randy Klabacka. Insitution: Utah Tech University. The capability to survive freezing temperatures is of crucial importance to ectothermic organisms inhabiting cold climates. The mechanisms that have evolved to minimalize the cellular damage incurred by freezing are of interest due to their applications in agriculture and medicine. fr10 is a gene identified in the wood frog (Rana sylvatica)that has been shown to reduce the cellular damage sustained in freezing temperatures. To date, fr10 has not been identified in any taxa beyond R. sylvatica and as such, the evolution of this novel gene remains ambiguous. Furthermore, the exact mechanism by which fr10 reduces freezing related damage has yet to be determined. We use bioinformatics scripts to scan 38 frog genomes to identify orthologs in divergent frog lineages across varying elevational and latitudinal gradients and examine the evolution of this novel gene. Understanding the evolutionary and ecological context of this gene can elucidate its function and may inform commercial and medical applications.
Using Technician Statistics For Productive Animal Care
Authors: Grace Moore. Mentors: Aaron Olsen. Insitution: Utah State University. AbstractAnimal care technicians for research labs have many responsibilities in order to maintain the integrity of a research project. However, it is difficult to determine what tasks will require most of a technician’s time and effort. Thus, it is proposed that tracking what each individual does during a given shift can allow supervisors and technicians to create an efficient schedule of how they will work and provide a standard for individuals to meet every day. This project consists of tracking in detail what an animal care technician does in a given “shift”, including observation hours, handling and restraining, working in different biosafety levels, cleaning and prepping caging, and changing the caging. This data collection, over several months of work, finds relationships between tasks, the frequencies of being completed, and the time they take. The key tasks of an animal care technician are observing, providing nourishment, maintaining clean environments for both the researchers and the subjects, and maintaining supplies and accurate records. Additionally, many hours are dedicated solely to observing the animals to ensure their health and safety. Moreover, cleaning and prepping of caging or supplies are completed more than half of the time during a given shift. Finally, working with a given species may require more time to complete a task (such as cage changing or cage preparation) compared to another species despite the task being the same. If each technician were to track their tasks and create a profile of personal statistics, they would be able to easily determine how long a task should take. Moreover, a supervisor would be able to determine if a technician is working more efficiently or even falling behind during daily tasks. Overall, these findings could be used to incorporate better time management or planning for institutions to know what more time or resources may need to be devoted to in order to maintain a safe environment, provide exceptional care for the subjects, and follow every protocol.
Computational analysis of cyclic aminoborane complexes that exhibit potential to act as hydrogen storage molecules
Authors: Amy Richards, Caleb Shelton, Jordan Colmenero, Mason Warenski. Mentors: Diana Reese. Insitution: Utah Tech University. Hydrogen gas has potential to be an excellent form of clean energy; unfortunately, hydrogen gas is difficult to store safely in its elemental form. Consequently, this research project investigated cyclic aminoborane complexes in which hydrogen (H2) can be safely stored. Utilizing computational methods, molecular dynamics simulations of four cyclic aminoborane compounds were performed using Q-CHEM 6.0, with a t-HCTHh density functional and cc-pVDZ basis set. Results were visualized using Molden 6.7 and VMD 1.9.3 then plotted using Gnuplot 5.2.8. Molecules selected for the investigation involve two constitutional isomers for both the 3- and 4- membered ring cyclic aminoborane complexes. The four molecules studied were (CH2)2NHBH3, (CH2)2BHNH3, (CH2)3NHBH3, and (CH2)3BHNH3, (hereafter 3N-B, 3B-N, 4N-B, 4B-N). Motions inherent in these molecules exhibited surprising differences. Specifically, in the 3-membered rings, the terminal borane (3N-B) exhibited more motion than the terminal amine (3B-N); however in the 4-membered rings (4N-B and 4B-N) the opposite occurred. Differences in these and other motions were quantified and qualified with respect to each molecule to gather data relevant to hydrogen storage potential.
Unveiling Cryptic Avifauna Diversity Using Nanopore DNA Sequencing: A Case Study in the Passerine Genus Emberiza
Authors: Ainsley E Parkins, Sylvia Lee, Elizabeth J Heath, Thomas Petitjean, Kyle Cink, Kyle Kittelberger, Cagan Sekercioglou, Rodolfo S Probst. Mentors: Rodolfo S Probst. Insitution: University of Utah. Biodiversity loss poses a significant threat, requiring accurate species assessment and conservation strategies. Non-invasive and cost-effective techniques should be prioritized, especially when dealing with threatened vertebrate fauna. By focusing on cryptic Eastern European species within the genus Emberiza (Passeriformes: Emberizidae), we designed an affordable molecular pipeline (including DNA primers and bioinformatics) integrating DNA barcoding with Nanopore sequencing to address species boundaries and provide conservation information for avifauna diversity. We conducted non-invasive DNA extractions from individual feathers of 192 specimens collected in the Aras River Riparian Corridor (ARRC), an important ecological hotspot in Turkey. By targeting the cytochrome oxidase subunit I (CO1) region, our goal was to differentiate Emberiza taxa and identify potential cryptic lineages while using cutting-edge next-generation sequencing (NGS) of multiplexed specimens with portable Oxford Nanopore Technologies. Our focal study examined boundaries in Emberiza subspecies (E. shoeniclus shoeniclus and E. shoeniclus caspia) with subtle morphological differences, utilizing DNA barcoding and Nanopore sequencing. We obtained 182 Emberiza DNA barcodes. Clustering analysis recovered a paraphyletic assemblage (n=32) and a clade, the latter recovering majority of the barcodes (n=150), separated by at least 8% genetic difference. In both cases, we observed a mixture of barcodes from the nominal subspecies and Caspian subspecies, suggesting that cryptic morphological characteristics are not reflected in genetic separation. Our results also suggest putative cryptic Emberiza species, highlighting the importance of the ARRC for conserving avian diversity. We demonstrate that our pipeline, integrating real-time Nanopore sequencing with non-invasive DNA barcoding, can be used for conservation initiatives (particularly aiding cryptic species identification). This study expands our knowledge of avian diversity and highlights the potential of NGS DNA barcodes as a powerful tool for aiding conservation efforts amidst biodiversity loss.
University Course Timetabling: From Sticky Notes to Automation
Authors: Alyssa Muller, Russ Ross. Mentors: Russ Ross. Insitution: Utah Tech University. University course timetabling assigns rooms and times to courses, considering instructor availability, curriculum conflicts, and quality of life considerations. Computing an optimal solution is computationally intractable. Researchers have refined approximation algorithms that yield far better results than the conventional pencil-and-paper approach used at many universities, including our own. Transitioning to an automated system can be disruptive and poses both real and perceived risks to an institution. The implicit knowledge that humans bring to the problem is hard to fully capture within formal rules that a computer can understand. Our research addresses the impedance mismatch between abstract solutions and the messy real world.In this project we build on prior research to fit the specific needs of our university. We will pilot our system with a set of departments in parallel with the traditional by hand process. We will analyze outcome quality through subjective assessment and quantitative comparison between human and machine generated timetables.
Synthesis and Characterization of Novel Pterostilbene Derivatives for Potential use as Therapeutics
Authors: Ashley Staten. Mentors: Jennifer Meyer. Insitution: Utah Tech University. Pterostilbene, a natural product found in blueberries and nuts, has been shown to be multifunctional. In low doses, it has been shown to be a potent antioxidant, while in higher doses, it has been shown to reduce cell proliferation, induce autophagy, and increase apoptosis. Recent literature has indicated that derivatives of pterostilbene can improve the functionality of pterostilbene in both cancer cell viability (reduction) and antioxidant capacity (increase). Previously, our group has synthesized and characterized the antioxidant capacity of a select group of pterostilbene derivatives, which have been functionalized to contain an additional alcohol and amine group. Most recently, we have synthesized a derivative containing benzylamine as the amine. This amine was chosen as benzylamine and drugs functionalized with benzylamine groups have been shown to have antiapoptotic properties. Currently, we aim to further characterize this novel pterostilbene derivative as we believe it has the potential to be utilized as a therapeutic in various disease states such as cancer and type 2 diabetes.
Rock Column Displacement in Zion National Park: A statistical Analysis
Authors: Michael Orr. Mentors: Md Sazib Hasan, Alex Tye. Insitution: Utah Tech University. Every year Landslides claim lives and cause billions in property damage. Zion National Park in Southwestern Utah has been monitoring a precarious rock column contained within the parks borders since 2019. The results of this rockfall will be damage to the land and historical structures located in the fall area. The data are collected by two instruments called vibrating-wire crackmeters that are connected from the cliff face to the pillar—these are electronic and provide a continuous feed of distance measurements. The project will be focused on analyzing a 4-year record of displacement of a precarious rock pillar from the adjacent cliff face from which it will eventually separate and topple onto a slope below. We will also be analyzing the environmental factors causing the movement of the rock column. Results of this modeling will help us better understand the driving forces behind rockfalls, possibly helping to mitigate future damage.
Introduced population of a unisexual lizard species in Southern Utah
Authors: Vicente Fernández Lara, Reagan McKee, Randy Klabacka, Angelina Romero. Mentors: Randy Klabacka. Insitution: Utah Tech University. Aspidoscelis (family: Teiidae) is a genus of lizards with many parthenogenic species found in North America. The parthenogenic species reproduce asexually, meaning all individuals in these species are female. The native range of Aspidoscelis neomexicanus (the New Mexico whiptail) is within the Rio Grande basin in New Mexico and Trans Pecos Texas. It has been reported outside its foreign range in northern Utah (Salt Lake area) as an introduced/invasive species, but has never been reported in southern Utah. We captured three individuals in Washington County that have the superficial diagnostic characters of Aspidoscelis neomexicanus. Using molecular genetics, we will test the taxonomic identity of this species and use phylogenetic analysis to determine the source population from its native range.
Hollow hair and how its structure helps big game animals thermoregulate
Authors: Taylor Millett, Wendy Schatzberg, Samuel Tobler. Mentors: Wendy Schatzberg. Insitution: Utah Tech University. This study delves into the intriguing world of hollow hair strands in animals, focusing on their role in thermoregulation, and the ability to maintain a stable body temperature in the face of fluctuating external conditions. While the Pronghorn antelope is widely known for having hollow hair strands among hunters and conservationists, little is known about their internal structure. Employing scanning electron microscopy (SEM), we explored the inner composition of these hollow hair strands and their contribution to thermoregulation. Our investigation centered on several notable North American big game animals, including Mule deer, Rocky Mountain elk, and Pronghorn antelope, all of which exhibit a unique adaptation: the transition between summer and winter coats. Through SEM analysis, we measured and compared the winter and summer coats of these animals to gain insights into how they effectively regulate their body temperatures during the extremes of hot summers and cold winters. These seasonal changes manifest in alterations in fur and hair thickness and length. Under the microscope, we unveiled the distinct topography of the inner structure of individual hair strands. Notably, our findings revealed that the inner hair structure contains larger hollow pockets in the winter coats of these animals. Our research thus sheds light on the role of these hollow structures in heat transfer and their pivotal contribution to the thermoregulation abilities of these remarkable creatures, expanding our understanding of their unique adaptations.
Optimization of Subcritical Water Hydrolysis of Microalgae Biomass for Clean Meat Production
Authors: Bradley Lawson, Lukas Keller, Marshall Burrows, Sam Perkins, Emery Wheeler. Mentors: Ronald Sims. Insitution: Utah State University. Subcritical water hydrolysis (SWH) is a developing method of processing organic materials/waste without the use of acids and alkali materials. SWH lyses peptides differently than acid hydrolysis and can produce Maillard products, which have various effects in cell culture. In this study, a batch-mode pressure vessel with a semi-continuous sampling apparatus will be used to optimize temperature and retention time for the hydrolysis of Chlorella vulgaris biomass for the development of serum-free (or low-serum) cell culture media. Hydrolysis will be done at temperatures from 140°C to 300°C and retention times from 5 minutes to 30 minutes. The SWH products will be assessed for their general protein content, lipid content, bioactivity, and carbohydrate content. These efforts will be of use to Upside Foods in its efforts to create sustainable and ethical cultured meat products.
mitochondrial genes of hybrid whiptail parthenogens
Authors: Dante Celani, Erin Bunch, Randy Klabacka. Mentors: Randy Klabacka. Insitution: Utah Tech University. Hybrid parthenogenic whiptail species have lower endurance capacity and mitochondrial respiration than their sexual parental species. These differences in aerobic performance may be due to reduced compatibility of variable gene products between the divergent parental genomes that are present in the hybrids. If this is the case, we expect to see genetic variation in mitochondrial-targetting genes. We performed whole-genome sequencing for four sexual whiptail species that are parental species to hybrid parthenogenetic species, and we mapped those samples to an annotated whiptail genome. We call gene variants and classify them as coding, non-coding, synonymous, and non-synonymous to understand which variants might contribute to phenotypic variation, and we discuss approaches to further elucidate the genetic mechanism behind the lower aerobic performance in hybrid parthenogens.
Temporal Decay of Memory Discrimination: Investigating Mnemonic Similarity Task with Extended Delays
Authors: Alexandra Osmond, Samantha Stabler, Sara Knowlton, Sam Chipman, Brock Kirwan. Mentors: Brock Kirwan. Insitution: Brigham Young University. Memory discrimination refers to the ability to distinguish between different memories or experiences based on their unique characteristics or details. The Mnemonic Similarity Task (MST) is a variant of a recognition memory task that assesses memory discrimination by asking participants to encode a series of objects in long-term memory and later discriminate between targets (repeated items), lures (similar but not exactly the same as previously seen items), and foils (novel items). The similarity of targets and lures is systematically varied resulting in five similarity levels (L1-L5). The goal of our research was to determine if individuals retain memory discrimination abilities after different periods of time exceeding 12 hours. Study recruitment was done on Brigham Young University campus for 18-25 year olds with no known neurological conditions (ADHD, major depression etc.). Thirty participants were tested in each group and they were asked to complete an immediate test. They were then asked to return to the lab for a second test after a 1-day, 3-day, or 1-week delay.Our results showed that the 1-week delay of the MST eradicated an individual's ability to discriminate between the lures of varying similarity. At a 1-day delay, individuals did lose some memory discrimination ability, but were still able to recognize the more distinct lures.
The Impacts of Distress and Perceived Social Support on Heart Rate Variability
Authors: Mikel Cressman, Jackie Richey, Dantzel Petersen Hancey, Bianka Geraldo, Kayden Barlow, Ainsley Lumsden, Olivia Hubble. Mentors: Patrick Steffen. Insitution: Brigham Young University. Heart rate variability or HRV is a measure of the amount of time between a person’s heartbeats and is related to their physiological response to stress. Social support is an important component of positive health outcomes and plays a part in mediating distress. More research is needed to better understand the connection between HRV, social support and distress. We hypothesize that those with low baseline HRV will have higher distress scores and lower social support than those with high baseline HRV. We also hypothesized that someone with high distress and high social support will have higher HRV than those with high distress and low social support. In this study, social support was measured using the Multidimensional Scale of Perceived Social Support (MSPSS), distress was measured using the Counseling Center Assessment of Psychological Symptoms (CCAPS), and baseline HRV was measured using a NEXUS biofeedback device using ECG leads on the collarbones and left rib. Our sample consisted of 236 college students (17-28 years old; 57.6% female; 85.9% white) drawn from an online recruiting software and student development classes. For the first hypothesis, we will use a MANOVA to compare both low and high HRV baseline with mean MSPSS and CCAPS scores. We will use an ANOVA to compare differences in HRV between those with high MSPSS and high CCAPS scores to those with low MSPSS and high CCAPS for the second hypothesis. We expect to find that those in the lower HRV group will have higher CCAPS scores and lower MSPSS scores when compared to the high HRV group. We also expect to find that participants in the high CCAPS and high MSPSS group will have a higher HRV when compared to the high CCAPS and low MSPSS group.
Contrasting Representations of the Berlin Wall in American and German Museums
Authors: Breanne Herrmann. Mentors: Paul Stavast. Insitution: Brigham Young University. The construction and preservation of the Berlin Wall in 20th century Germany has perpetuated a social legacy perceived differently in various countries. Although the historical facts are unchangeable, their representation by institutions in each country varies and reveals their unique participation with the German Democratic Republic. This paper analyzes the portrayal of the Berlin Wall and surrounding GDR events in museums in both Berlin, Germany and American cities. Die Mauer, The Wall museum at Leipziger Platz in Berlin serves as a model of the German perspective. Its focus on the logistics of the Berlin Wall and the social impact in Germany and surrounding satellite countries is compared to the portrayal in American museums, which assume a more general approach of the Berlin Wall as a symbol of greater political struggles. This includes a consideration of locations of museums in America as well as information presented in the exhibits. Identifying differences between museums in these two countries provides insight into the experience of each during the GDR period and subsequent social perceptions of the Berlin Wall's history.
Counting Crowds: An Analysis of Population 'Top-Outs' in Counties Across the United States
Authors: Maisie Baird, Hugh Kerry, Samuel Otterstrom. Mentors: Samuel Otterstrom. Insitution: Brigham Young University. Throughout the history of the United States, populations have experienced continual fluctuations across its diverse landscape. Although much research has focused on the growth and decline of cities and counties, many of these studies have focused on the positive population growth in larger cities and counties, insomuch that scholars and the public might have an incomplete or skewed view of the historical population geography of the country. However, this paper presents a broader perspective, including times of both population growth and decrease across regions and multiple decades and showing how these trends do not follow strictly positive trajectories nor exhibit isolated randomness. This paper will focus on specific examples of counties across the United States, highlighting why there were population ‘top-outs’ in these areas. For example, Sheridan County, Nebraska experienced a population boom at the onset of World War I, as it became the United States’ primary source of potash. However, at the end of World War I, potash could again be cheaply imported from Europe so, Sheridan County lost its booming industry, resulting in population decline. This research delves into the concept of population ‘topping out’ in counties, emphasizing a comprehensive understanding achieved through a holistic framework that accounts for geographic, regional, economic, and temporal aspects.
Influence of Childhood Levels of Self Control on Adolescent Sleep Outcomes
Authors: Jacalyn Richey, Hayley Pierce. Mentors: Hayley Pierce. Insitution: Brigham Young University. Good sleep habits and effective self-control are important components of successful functioning. Unfortunately, chronic sleep loss is a common concern in adolescence as both their autonomy and demands on their time increase. This loss of sleep impacts neural and physical development and is connected with increased mental illness, delinquency, and academic difficulties. Self-control may aid in the prioritization of sleep by curbing impulsive desires and improving decision making around adhering to bedtimes. This study drew on data from the Future of Families and Childhood Wellbeing Study (FFCWS) measuring self-control at age 9 and sleep outcomes at age 15 (N = 3,444; 48.7% female; 49% Non-Hispanic Black). Multivariable analyses indicated that self-control showed statistically significant relationships with social jetlag, falling asleep, and having a regular bedtime after controlling for several relational and economic factors. Improving childhood self-control habits and skills through modeling and instruction may lead to increased positive sleep outcomes, which may increase healthy development in adolescence and beyond.
How Depression Affects “Feeling the Spirit” in College Age Latter-Day Saints
Authors: Nadia Mando, Josh Shaeffer, Jared Nielsen. Mentors: Jared Nielsen. Insitution: Brigham Young University. Background: “Feeling the Spirit” is an important experience for many active Latter-Day Saints. Feeling the Spirit is often defined by the presence of positive, even if minor, feelings which are perceived to be interactions with deity. However, some Latter-Day Saints struggle to feel the Spirit which can be distressing to them. Recently, LDS leaders have focused on understanding how depressed individuals might experience “feeling the Spirit” differently.Objective: Our pilot study sought to understand the relationship between depression and the frequency and strength of spiritual experiences.Methods: We used the Depression Anxiety Stress Scale-21 (DASS21) to measure depression. We created two separate measures to inquire how frequently and strongly participants felt the Spirit in the last six months. We also created a task asking participants to read multiple scripture passages and report how strongly they felt the Spirit while reading each passage. Responses to each passage were averaged to create a state measure of feeling the Spirit. We hypothesized that higher scores of depression would predict lower scores on feeling the Spirit for all three measures.Results: We found that scores on depression (M=11.388, SD=7.844) significantly correlated to frequency (M=3.683, SD=0.760, r= -0.352, p<.001) and strength (M=4.004,SD=0.628, r=-0.453, p<.001) of the Spirit. However, depression scores were not significantly correlated with our state measure (M=2.384, SD=0.903, r=-0.192, p=0.078). In our regression models we found that depression is a significant predictor of both frequency (R2= 0.091, F(1,83)=8.347, p=.005, =-0.029, p=.005) and strength (R2=0.158, F(1,83)=15.523, p<.001, = -0.032) of feeling the Spirit but, not of feeling the Spirit during the scripture task (R2=0.003, F(1,83)=0.236, p=0.628, = -0.463).Conclusion: These findings suggest that depression may affect how LDS persons experience their relationship with God. Our findings take a step toward explaining why some individuals may experience difficulty in “feeling the Spirit”. Future studies are necessary to elucidate these preliminary findings.
Examining the Effects of Service on Male-Coded Committees on the Reelection of Female Legislators
Authors: Annabelle Crawford. Mentors: Celeste Beesley. Insitution: Brigham Young University. Women who run for office do not fit societal leadership expectations, which hurts their chances of being elected (Bauer 2020; Eagly and Karau 2002). Women who serve in male-coded positions face backlash for confronting issues that are incongruent with stereotypically feminine attributes (Davidson-Schmich, Jalalzai, and Och 2023). I theorize that social role incongruence is exacerbated for women who serve on male-coded legislative committees. Therefore, I predict that women who serve on male-coded committees are reelected less frequently than women who do not serve on male-coded committees. However, I propose that women with leadership roles on male-coded committees are members of the political elite and therefore will be reelected at rates similar to other legislators. This research will employ a census of female legislators from both houses of the U.S. Congress who began their tenure between 1993 and 2022, using data compiled by the Congressional Research Service. Whether a committee is male-coded or female-coded (or gender-neutral) will be determined based on whether men or women dominate the industry associated with the committee. Social role incongruence will be measured by analyzing public perceptions of female legislators during the time they served on a male-coded committee. I will use regression analysis to determine the effect of serving on a male-coded committee on a woman’s gender role incongruence. I will then compare the proportion of reelection races won by women who served on a male-coded committee to the proportion of races won by women who served on a female-coded or gender-neutral committee using difference in proportions tests. I will also compare the electoral success of women who held leadership roles on male-coded committees to the electoral success of other legislators.
Investigating the Effectiveness of Incentivized Efficient Watering Practices in 5 Western States with Focus on Utah
Authors: Ashley Swensen, Ruth Kerry. Mentors: Ruth Kerry. Insitution: Brigham Young University. The American West has been experiencing severe drought for over 20 years with changes in climate. Also, the population in certain states has greatly increased putting increasing pressure on the limited fresh water supply. While the agricultural sector is the biggest consumer of freshwater, much is also used to irrigate turfgrass lawns in urban residential areas. The EPA has estimated that about 50% of household irrigation water is wasted by temporal and spatial misapplications. Temporal misapplications can be reduced by using smart sprinkler controllers that take into account recent weather when scheduling irrigation timing. This research investigates people’s willingness to implement more efficient watering practices with and without incentives. It also investigates how an average water bill for a quarter acre lot varies between states and between cities within 5 states in the semi-arid west (AZ, CO, ID, NV, UT). Following comparison of average water bills between states and cities, the potential savings from taking advantage of water saving incentives offered by cities is investigated. The research found differences between the genders and aridity zones in terms of the types of incentive that are favored and likelihood of utilizing methods to improve irrigation efficiency. Water bills were the highest in AZ and lowest in ID and UT. The low water bills in ID are likely associated with the greater prevalence of agriculture in that state than the others studied. Most UT cities had incentives for Smart Sprinkler controllers or to xeriscape. However, in other states several cities did not have incentives for installing Smart Sprinkler controllers. We conclude by trying to assess whether incentives or disincentives are more effective at reducing water use by residential customers.
The Physiological Effects of Capitalization in Ambivalent Marriages
Authors: Abigail Williams, Kelsie Minga, Abby Baker, Ella Sieg, Olivia Harris. Mentors: Wendy Birmingham. Insitution: Brigham Young University. Background: Research has found positive health benefits in the communication of positive events (capitalization) with others who respond with curiosity and enthusiasm. An expectation is that one’s spouse would respond in such a way. And indeed, if one has a highly supportive spouse this is likely the case. However, not all individuals have a highly supportive spouse and may have a spouse whose behavior is more ambivalent. Such ambivalent, or unpredictable behavior may lead to worse physiological outcomes when sharing good news including increased risk for cardiovascular events. Blood pressure should dip 10-15% overnight (“dipping”). A less than 10% dip is associated with higher cardiovascular disease risk. We looked at capitalization and dipping in ambivalent and supportive couples. Interpersonal Theory asserts that positive or negative behaviors from one spouse can elicit similar responses from the other spouse. Thus we looked at capitalization and dipping for spousal ambivalent behavior, and one’s own ambivalent behavior. Ambivalent behavior, whether one own’s or one’s spouse’s can also impact the quality of the relationship. Methods: 90 couples were recruited from a local university, social media, and the community. Participants completed measures of marital quality. They were fitted with an ambulatory blood pressure monitor for 24 hours. The monitor took a reading every 30 minutes during the daytime and once an hour overnight. Participants completed a daily diary entry each time the monitor took a reading during the daytime which included questions regarding their present activities (e.g., posture, current emotions, food consumption). Participants returned the equipment the following morning and were compensated. Results: Ambivalent behavior was associated with worse relationship quality measured as responsiveness, both in terms of one’s own behavior, and one’s spouse’s behavior. We found no association for systolic blood pressure (SBP) dipping for individuals whose spouse’s behavior was viewed as ambivalent, however diastolic blood (DBP) dipping was in the opposite direction showing ambivalent spousal behavior was associated with healthier dipping. Of interest, when one reported their own behavior as ambivalent when their spouse was sharing good news, their SBP dipping was unhealthy. Conclusions: Spousal ambivalent behavior seems to be less important than one’s own behavior on SBP dipping. This may be because one is generally aware of their own behavior and understands that such behavior is problematic for the relationship. This awareness, however, may come with a cost for their health as well as their relationship.
The Effects of Anterograde Amnesia on Mnemonic Discrimination: A Single-Subject Case Study
Authors: Sara Knowlton, Sam Chipman, Samantha Stabler, Alex Osmond, Brock Kirwan. Mentors: Brock Kirwan. Insitution: Brigham Young University. The hippocampus is a brain structure involved in recognition memory and pattern separation, or the ability to disambiguate potentially interfering or overlapping memory representations. The Mnemonic Similarity Task (MST) has been used to assess memory specificity by asking participants to encode a series of objects and later perform a recognition memory test with repeated items (targets), similar but not exact repeats (lures), and novel items (foils). In a single-subject case study, we utilized the MST in evaluating recognition memory over various delays of a subject with anterograde amnesia. Subject MC is a healthy young adult female who underwent a unilateral amygdalohippocampectomy as a treatment for epilepsy. This procedure included the complete removal of her hippocampus in the right hemisphere, which we verified with magnetic resonance imaging. In addition to the MST, we compiled a battery of neuropsychological tests, including the Wide Range Assessment of Memory and Learning (WRAML-3), the Delis-Kaplan Executive Functioning System, and the Wechsler Abbreviated Scale of Intelligence (WASI-II) to more holistically assess MC’s memory deficits. Preliminary analyses showed significantly lower lure discrimination when compared with control subjects, indicating deficits in pattern separation. Further analysis of MC’s MST results are being conducted to evaluate her broader ability for recognition memory.
Relationship Between Income and Lifespan Using Historical Data
Authors: Joseph Price, Britton Davis, Alexander Jenks. Mentors: Joseph Price. Insitution: Brigham Young University. Modern data show a strong positive relationship between community-level average income and life expectancy in the United States. We compile a dataset including place of residence, lifespan, and a proxy for income for 27 million unique individuals from the 1900-1940 censuses. In contrast to the pattern in modern data, we actually find that individuals living in an enumeration district with the top quartile of our proxy for income experienced shorter lifespans than those living in the bottom quartile. We show that this negative relationship was largest in the 1900 census and slowly shrank over the next four decades showing how this negative relationship evolved to the positive relationship we see today.
Sodium Reduction Policy Response
Authors: Jacob Hutchings, Harrison Li. Mentors: Joe Price. Insitution: Brigham Young University. We examine the effectiveness of federal school nutrition policies and regulations; specifically, the recent transitional targets for sodium reduction announced in 2021. We performed web-scraping to gather over 5 million observations representing every U.S. state and over 2000 school districts and then constructed a weekly average of sodium served in school lunches by district. Using this data, we apply OLS regression with district fixed effects and find that the policy caused a significant, one-time reduction in weekly average sodium of approximately 220 mg. We further explore this result by looking at heterogeneous responses to the policy by political partisanship. We find that there is a significant, partisan response to the transitional targets. Lastly, we consider an adverse effect of sodium reduction by looking at potential sodium substitutes in school lunches. Contrary to expectations, our data shows a statistically significant correlation between decreased sodium levels and decreased sugar levels. To conclude, sodium reduction policies reduce sodium and other harmful nutrients but may be less effective across party lines.
Frim Patron To Partner: Accomplishments of Museum-Armature Archaeology Relationships
Authors: James Hall. Mentors: Paul Stavast. Insitution: Brigham Young University. As of November 2023 the Utah Statewide Archaeological Society's social media pages have close to 4 thousand followers, which shows that interest in Archaeology. From 1955 to around the mid-90s USAS members contributed greatly to the archaeological research and the protection of archaeological sites and the materials created by those people that had called what is now Utah home. Through the partnership of Universities and Museums they had great success and this poster aims to highlight some of those successes and push for Museums to continue facilitating these opportunities.
Experience impact and the influence of novelty, social connection, and positive emotions on tourist experiences
Authors: Braden Bringhurst, Michael Ray, Megan Barbier, Ellie Smith. Mentors: Peter Ward. Insitution: Brigham Young University. In travel, the interplay of social connection and novelty profoundly shapes the impact of peoples' experiences. This study examines novelty, positive emotions, social connection, and the impact of the experience on participants through the lens of the Self-Expansion Theory. Novelty is defined as the process of experiencing or encountering something different to the objects regularly encountered in everyday life. Social connection is defined as the affective and cognitive perception of interrelatedness within social groups, encompassing emotional responses like feeling understood and valued. Positive emotion is defined as pleasant multicomponent response tendencies, including joy, interest, contentment, pride, and love. Experience impact is defined using the experience typology. This typology starts with prosaic experiences or ordinary parts of life. The next progression is memorable experiences which introduce felt emotions into the experience. The next stage is meaningful which introduces discovery as well as emotions into the experience. The final stage is transformational which introduces a desire, or actual personal change happening to the experiencer as well as emotions and discovery. To measure the various stages of the experience typology framework, we use the Experience Impact Scale to identify the personal impact of a tourist experience. These various constructs are explored through the perspective of the Self-Expansion Theory which states that people seek to expand their potential efficacy, perspectives, and sense of self through the development of relationships. This study utilizes this theory by looking into novelty’s effect on the impact of an experience through the lens of Self-Expansion Theory with respect to social connection and positive emotions as mediating factors. The data were analyzed using structural equation modeling in AMOS v 28. Models were gradually expanded on, starting with the initial relationship between subjective novelty and experience impact. Later, a mediating model was considered that incorporated subjective connection and positive emotions as mediating effects on experience impact. The model examining the relationship between subjective novelty and experience impact as mediated by subjective connection and positive emotions had an acceptable model fit. The significant factors that contributed to the model were subjective novelty which had a standardized regression weight of .264, subjective connection which had a standardized regression weight of .222, and positive emotions which had a standardized regression weight of .256. The model explained 16% of the variance within experience impact. This study demonstrates that novelty influences social connection which influences positive emotion which positively impacts the tourist experience. People designing experiences should create a novel experience for the experiencer that evokes positive emotions and facilitates social connection. Future research could explore the relationship between the novelty of location and activities associated with tourist participation and the impact on the experience.
Information-Gaining Moves in Game Theory
Authors: Kevin Young. Mentors: Gretchen Ellefson. Insitution: Southern Utah University. The concept of competition is not new to humans, nor is it simple. Countless philosophers throughout the years have approached competition in many ways. One of the core subjects that philosophers have used to discuss competition is that of decision theory. Decision theory, in short, discusses the best things to do. Game theory, a subset of decision theory, discusses the same within the context of games, or in other words, strategy. Both of these subjects have been used to evaluate how best to win games of chance, games of choice, and games in between. I work to explore the concept of making moves to gain information, seeking to learn another person’s moves before you make your own. This subset of moves deserves recognition, especially within the modern eras of competition.
Preferential Capture of Bacteria Using pDA-coated Magnetic Nanoparticles
Authors: Alyson Camacho, Camille Bryner, Bowen Houser. Mentors: William Pitt. Insitution: Brigham Young University. Current methods for the diagnosis of bacterial infections require time consuming cultures. To prevent deaths caused by sepsis, faster methods for bacterial identification and antibiotic susceptibility methods are urgently needed. Necessary to these faster methods is the ability to separate and concentrate bacteria. While techniques such as filtering and centrifugation have been explored for this purpose, we propose an innovative approach. Polydopamine (pDA) is a remarkably adhesive polymer that has been used to create antibacterial and anti-biofouling coatings on medical devices. In this study, however, we show how magnetic nanoparticles (MNPs) coated with pDA can selectively bind and remove specific bacteria from suspensions. For these experiments, pDA- MNPs were added to suspensions of bacteria mixtures and allowed to bind for 10 minutes. The MNP/bacteria mixture was then placed on a holder with a magnet which collected the MNPs on the side of the test tube. Finally, the bacterial supernatant was carefully removed and plated. Capture efficiency of the beads was calculated and it was shown that the pDA-coated MNPs preferentially isolated S. epidermidis from a suspension of both S. epidermidis and E. coli. This approach holds great potential for separating bacteria from clinical samples like urine or blood, enabling faster and more efficient diagnosis of bacterial infections.
De novo genome assembly and annotation of Curculio sp. (Coleoptera: Curculionidae) provides insight into diapause evolution
Authors: Daniel Davis, Paul B Frandsen. Mentors: Paul B. Frandsen. Insitution: Brigham Young University. The nut and acorn weevils of the genus Curculio (Coleoptera: Curculionidae) are a diverse group of beetles with a unique life history. A female weevil uses her rostrum (snout) that is about the length of her body to dig into the flesh of a developing hard-shelled seed and lay her eggs inside where they can safely develop into larvae. After the grown larvae exit their seeds, they spend one or more years burrowed in the soil near host trees. During this time they enter diapause, a state of suspended development to minimize the energy that they expend. Studies indicate that a major purpose of this behavior is to align their adult emergence with masting events (large scale seed production every 2-5 years) of their host trees. Between, and even within, Curculio species, there is significant variation in diapause lengths and behaviors (Higaki, 2016). This wide array of adaptive behaviors is a result of the coevolution between these insects and their various hosts. Here, we present a high quality genome of a Curculio species. With this genome, we discuss the genetic and evolutionary factors that have given rise to this unique life history of Curculio and future plans to compare the genomes of multiple Curculio to further unravel this mystery.
Diversity and community in macroinvertebrates of Timpanogos Cave, Utah
Authors: Erika Frandsen. Mentors: C. Riley Nelson. Insitution: Brigham Young University. The Timpanogos Cave System consists of three caves in American Fork, Utah. In 2003, the National Park Service (NPS) commissioned Dr. Riley Nelson and laboratory students to conduct a survey of the diversity and abundance of terrestrial arthropod species found within the caves. Over the two years they sampled in Timpanogos Caves, they identified 55 species of macroinvertebrates in an unpublished report.In the 20 years since the last sampling, many environmental factors could’ve changed in these caves, contributing to an overall decrease in both abundance and biodiversity of fauna. Therefore, we repeated the sampling done by the Nelson lab in 2003-2004 for the duration of May 2023-October 2023 to compare the two time periods’ macroinvertebrate communities.The six taxonomic families found most abundantly in 2003-2004 continue to be the most abundant families found in 2023. Additionally, a new taxon of cave beetle Rhadine sp. not found 20 years ago was discovered early in our sampling of 2023. Despite the increase in visitation over the past 20 years, at this stage in the analysis, the macroinvertebrate community within the caves seems to have almost no loss of diversity. Additionally, the appearance of new taxa indicates that there is still more life and diversity within the caves left to discover.
The Portrayal Of Reality Through The Lens Of The Astonishing World Of Studio Ghibli
Authors: Emma Manley. Mentors: Johnathan Chidester. Insitution: Southern Utah University. This presentation will be about the indescribable magical Studio Ghibli movies. Specifically, I will be analyzing how these movies are great at representing life. From the weird, outstanding world of Chihiro’s ghost town from Spirited Away, all the way down to the simple little Iron town of Princess Mononoke. These movies are amazing, not just for their remarkable art work and lovable characters, but for the relatability and symbolism of these stories. These stories are weird and strange, but so is everyone. We are human. A complex being that is evolving every single day. We have creative minds in all sorts of differing ways, and these movies are great at explaining this; they are complex, imaginative, and life-like. I will not be defining every single Studio Ghibli movie, but I have chosen a few movies I wish to expand on. My first one is The Wind Rises. This movie is based on a true story about Jiro Horikoshi, the designer of the A5M fighter aircraft. This story goes into detail about his life: his failures, his trials, and the hardships he endured. The second one is Spirited Away: this story is Studio Ghibli's most famous work. This is a story of a little girl who gets trapped in a spirit world all alone. Along her journey she makes new friends, discovers new ideas, and is able to discover who she is. The final movie is Princess Mononoke: Princess Mononoke is Studio Ghibli's masterpiece. The young warrior Ashitaka is strucken with a curse and will soon die. This story is about him getting rid of his curse, discovering how to make peace and stop hate.We should know and understand these movies, not because they are just films and that I like them, but because they are innovative and original. With these movies we jump into a creative world that we can make our own. These movies are relatable in every sense because they are just like us: weird, quirky, and just so compelling. We feel a connection to the trials that each character has because in a sense we all have been through something of the same. These movies represent life in a silly way that I wish to know more about. I will expand on the way these movies are made, the theme and writing, and the characters that we relate to.