Authors: Nathan Anderson
Mentors: Zoe Thompson
Insitution: Utah Valley University
The pro-opiomelanocortin (POMC) gene is expressed in the hypothalamus and the pituitary gland. The products of this gene are important for food intake and energy expenditure. Mutations in the POMC gene can lead to obesity, excess hunger, and infertility. We are studying a mouse model of POMC deficiency in order to better understand what causes the infertility. In this experiment, we want to study the estrus cycle of mice who have the POMC mutation. The estrus cycle is similar to the menstrual cycle in humans. Specifically, the estrus cycle can be divided into four different stages: estrus, metestrus, diestrus, and proestrus stages. To determine which stage of the cycle the mice are in, we analyze the cells that are present in a vaginal smear, which is collected by a pipet smear technique, and then can be viewed under a microscope. This technique consists of filling a pipet with 0.9% saline solution and flushing this into the vagina of the mice. The cells lining the vagina will be washed off and then collected back into the pipet. These cells will then be added to a slide where they will be looked under a microscope and analyzed based on the number of cornified epithelial cells, leukocytes, and nucleated cells that are present. We have taken pictures of these smears and are analyzing them to determine what stage of the cycle the specific mouse is in at that time. Data was collected over a several week period. Since the POMC gene is absent in the mice being studied, we expect the estrus cycles in the mice to be abnormal. Based on our results, we will continue our research into looking at the specific hormone levels of the mice.