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Fine Arts

Unlocking the Mysteries of Glacial Watersheds: Tracing the Path of Water Chemistry Over Time and Space

March 21, 2024 12:00 AM
Authors: Miaja Coombs, Greg Carling. Mentors: Greg Carling. Insitution: Brigham Young University. Our research delves into the intricate relationship between glaciers and Alaskan rivers. We embarked on an extensive study across Southcentral and Interior Alaska, spanning various mountain ranges, to examine how glaciers affect the water chemistry in these regions. From small cirque glaciers to expansive valley glaciers and sediment-covered glaciers, we collected data from river sites extending from glacier termini to the ocean or larger river systems. Over a span of two years, our weekly and monthly samples shed light on the complex interplay of elements, isotopes, and seasonal variations in water sources within these proglacial rivers and streams. Our findings reveal the dynamic nature of glacier-influenced watersheds, especially in the context of a changing climate.

Ancestral Puebloan Ceramics Technology and Vessel Properties at Alkali Ridge Site 13

March 21, 2024 12:00 AM
Authors: Carolina Corrales. Mentors: James R. Allison. Insitution: Brigham Young University. This research analyzes ceramics found at the Alkali Ridge Site 13 in southeast Utah. The information generated with this research will allow us to know more about the technological choices of the Early Pueblo I people who lived at this location in the late A.D. 700s. The methodology will examine rim sherds through refiring and porosity tests. Refiring small sections of the sherds will provide initial information about the chemical composition of the clay used to create the vessels. The porosity tests should help determine the pieces' physical properties linked to different technologies. The combination of all these data will show differences in raw materials and their impact on the constitution of the vessels. The database obtained will allow us to statistically compare information from red, grey, and white wares, identifying patterns in size, shape, kind of material, and the technology used for each type.

Re-Membering and the Role of Community In Exorcism In Toni Morrison’s Beloved

March 21, 2024 12:00 AM
Authors: Jen Hansen. Mentors: Nicole Dib. Insitution: Southern Utah University. Toni Morrison’s contribution to Gothic horror with Beloved expands the tradition into the history of human enslavement, specifically in the context of American history. She reconceptualized ‘remembering’ as both the conscious awareness of the past and the literal reassembling of members of the body, and by extension the family and the home. Morrison coined the term “rememory” in reference to the intentional act of recollection performed by an entire community. The characters of the novel are haunted in several ways and each haunting is only exorcized or overcome through acts of communion, or rememory — many of which are symbolically religious even if not sanctioned as such. The main character is a woman named Sethe, who is haunted by the ghost of the daughter she murdered rather than allow to be taken as a slave. The ghost of Beloved represents the return of the repressed trauma of her death and the connection to Sethe’s previous life in captivity. In order to exorcize Beloved’s ghost and free themselves from her oppressive presence, Sethe and her living daughter, Denver, must re-member the broken family structure within their home, and rememory the traumatic past with the support of the community in order to heal. American Gothic traditions in literature have long been used to reflect on anxiety, discrimination, and disempowerment related to the Other. In this novel, Morrison uses that tradition to give shape to the culturally specific legacy of slavery in America. In this presentation I examine the novel’s Gothic elements and the ways the community plays the part of the exorcist as an essential advocate for the physical and emotional survival of Sethe and Denver.

Measuring shear wave speed in tendons using low-cost accelerometers on a flexible PCB with an Arduino microcontroller

March 21, 2024 12:00 AM
Authors: Eli F Smith, Christopher Dillon, Matthew S Allen. Mentors: Matt Allen. Insitution: Brigham Young University. Background: Shear wave tensiometry offers a method to measure in vivo tendon tension, crucial for inferring applied loads on tendons. However, existing equipment for this purpose is costly and lacks mobility, limiting the ability to study a larger cohort of subjects engaged in various physical activities. Goal: This research aimed to assess the viability of utilizing low-cost digital accelerometers in conjunction with an Arduino-based microcontroller for shear wave tensiometry. Approach: This work employs surface-mounted accelerometers on a custom flexible printed circuit board (PCB), so that even spacing can be maintained between the accelerometers without interfering in data collection. To test the system and verify its ability to acquire measurements at a high enough rate, the PCB was connected to a shaker driven with a known sinusoidal signal. The flexible PCB was held in place on the Achilles tendon using athletic tape with a tapper placed on the tendon to send a shear wave through the tendon. Results: The results obtained to date compare the accuracy of the proposes system relative to the current system, which uses instrument grade accelerometers. Results obtained to date on the tendon reveal the degree to which the PCB interferes with the measurements, and suggest possible modifications to improve future designs. Conclusion: To attain valid shear wave tensiometry data, further iterations of the flexible PCB design are needed. Moreover, employing more powerful microcontrollers capable of accommodating the requisite sample rates is necessary for an improved system.

Victor LaValle’s Destroyer: Comic as a New Medium for Black Narratives

March 21, 2024 12:00 AM
Authors: Shauri Thacker. Mentors: Nicole Dib. Insitution: Southern Utah University. The representation of Black narratives within the medium of comics and graphic novels has been and continues to be sparse compared to the focus on white characters and stories. In recent years, however, a new paradigm of Black representation has been appearing in comics and comics studies. Emerging from Black Lives Matter movement discourse about police brutality and taking a new angle on the “organic black protest tradition” is Victor LaValle and Dietrich Smith’s 2017 graphic novel Destroyer. Their visual narrative follows the last descendant of Frankenstein—Dr. Josephine Baker—and her endeavors for justice after the murder of her son Akai as her plotline weaves with the original Frankenstein creature’s quest to subdue humanity (Rickford, 35). In this paper, I will perform a close reading of this work using the theoretical lens of critical race theory and its intersection with feminist theory. My reading will prove that LaValle and Smith’s comic—though it does not have the intent of treating Black women as a “unitary and monolithic entity”—increases a reader’s focus on mother and doctor Josephine Baker in order to portray her rage and grief over Akai’s death at the hands of a police offer (Nash, 8). This analysis of Dr. Baker’s character, combined with LaValle’s usage of allusions and intertextuality, subverts the stereotypically white comic narrative by portraying the lived reality and precarity of many Black individuals within the United States. Through this reading, I will demonstrate that the medium of comics allows for a multifaceted depiction of Black narratives and a new form of literary activism.

The Impact of Augustine’s Theory of Evil on Latter-Day Saint Teachings

March 21, 2024 12:00 AM
Authors: Miranda Judson. Mentors: Mike Ashfield. Insitution: Utah State University. This research explores the relationship between Latter-Day Saint views and classical Christianity through the lens of the problem of evil. More specifically, this research looks at the implications both Augustine’s theory of evil and Latter-Day Saint theology have on each other and the impact that would occur should certain aspects be accepted by Latter-Day Saints. I argue that Latter-Day Saints should accept an interpretation of doctrine through the privation theory of evil in order to avoid potential threats to God’s goodness and the Plan of Happiness. Understanding Latter-Day Saint doctrine through this lens will better situate further research in the broader context of Christianity. Furthermore, accepting this theory will allow for a deeper understanding of the premortal existence and postmortal states. This deeper understanding is beneficial for both adherents to and researchers of Latter-Day Saint teachings. Finally, I argue this interpretation does not pose a threat to the doctrine of “opposition in all things.”

The Folklore of the Ghastly Menace

March 21, 2024 12:00 AM
Authors: Matilda Gibb. Mentors: Ami Comeford. Insitution: Utah Tech University. Prior to 1930, cannabis was used frequently in therapeutic practices and was found in many over the counter health products. However, around this time what has been dubbed the “pot panic” began infecting American citizens. It was then the film Reefer Madness came onto the scene, exacerbating the public’s trepidation over the allegedly morally degrading effects cannabis inflicted. Putting melodrama at the forefront, the film exploits societal queasiness over sex, drugs, violent crime, and immigration to push its propagandist agenda. The fear mongering rhetoric and imagery used effectively poisoned the public and altered societal perceptions of medical and recreational cannabis use. The goal of this project is to analyze the rhetorical argument presented in the film and address its broader reach and effects. Lastly, I will argue that the message presented in Reefer Madness was not based on any logical argument; it was based on folklore.

More Than Accommodating

March 21, 2024 12:00 AM
Authors: Natalya Misener, John Wolfe. Mentors: John Wolfe. Insitution: Utah Tech University. In our effort to improve education and create a more inclusive learning environment, Natalya Misener and Professor John Wolfe from Utah Tech University are exploring the experiences of neurodivergent students in classrooms. Our goal is to better understand, support, and empower these students, challenging the way things are typically done. We believe it's important to understand that these conditions come in a variety of forms and affect both students and professors in the classrooms in ways that are not currently being discussed, especially as many minorities, especially women and people of color, go undiagnosed and unaccommodated for a variety of reasons. We believe that by educating professors and students alike on how classroom environments can be more supportive of neurodivergent students, we can benefit the entire classroom, including ‘typical’ students. To make this happen, we believe it's important to rethink how we organize our classes, assignments, and interactions, so that we don't have to rely too much on services like the Disability Resource Center (DRC). We have practical suggestions, like adjusting assignments and deadlines and creating a comfortable classroom environment. It's also crucial to be clear in our expectations and listen to what the students need. We ask some important questions, like whether students should consider their instructors' limitations and how students see disabled instructors in their journey to graduation as well. We call for a shift beyond just accommodating neurodivergent students. We want to encourage educators to think about how they can better support all students in their classrooms. It's urgent that we change how we approach neurodivergent students- in order to create a more inclusive, supportive, and successful environment for us all.

Ethical Analysis of Web3 and Decentralized Internet

March 21, 2024 12:00 AM
Authors: Karli Kallas. Mentors: Jared Colton. Insitution: Utah State University. For this presentation, I will present a care ethics analysis of Web3 and decentralized internet, with a specific focus on privacy concerns and the increase of internet scams due to the lack of regulation and accountability. Ethics of care argues that there is moral importance in promoting the well being of care givers and receivers in a network of social relations, recognizing that all human life is co-dependent. It is important to note that care ethics was not designed to engage with such large networks of relationships, I will be expanding it to apply to the situation, so it gives us one new way of viewing the situation. I will expand on this ethical lens by including points from care ethics such as the empathy in the design of Web3, and responsibility for online communities. My research explores the intricate web of relationships and responsibilities in the decentralized environment, aiming to shed light on the vulnerabilities within internet scams in contrast to the benefits of cryptocurrency. I examine the moral dimensions of these scams, considering the impact on individuals and the broader digital community. The emphasis on care ethics demonstrates the interconnectedness of actors within Web3, as well as the need for a collective commitment to certain forms of innovation.

Theology and Prosperity of Women Within the Latter-Day Saint Church: Exploring Utah’s Religiosity Effects On Feminism

March 21, 2024 12:00 AM
Authors: Alexis West Salinas. Mentors: Johnathan Chidester. Insitution: Southern Utah University. There is a growing need to further understand the psychological and physical effects of religiosity among female-identifying members within the LDS church. According to recent studies, as of June 2023, Utah has a Mormon population of 68.55%. Within these statistics, about a fifth of LDS members reported that they have or are currently taking medication for depression. The article also states that 27% of LDS women have depression compared to the 14.5% of LDS men. The church has an infamous reputation with the demonization of mental illness, especially with women. Given the history of society’s treatment of women, women are at an increased risk of suffering within the LDS church. It is important to have the best interests of LDS women in discussing the effects religiosity has on female members within Utah. This paper will analyze the well-being among women of the LDS church as well as assess the specific effects religiosity within Utah may produce surrounding the topics of purity culture, societal expectations, job opportunities, and relationships. This paper will also touch on topics relating to Utah education, LGBTQ+ women within the church, as well as compare and contrast gender roles and sociology. Composing a formulated understanding of these relationships paves the pathway forward in implementing accommodating tools for women within the LDS church in mental health and spirituality.

Reducing the Cytotoxicity of Polyethylene Glycol Diacrylate Microfludic Devices Using an Isopropyl Alcohol Washing Method

March 21, 2024 12:00 AM
Authors: Parker Johns, Chandler Warr, Gregory P Nordin, William G Pitt. Mentors: William G Pitt. Insitution: Brigham Young University. Polyethylene glycol diacrylate (PEGDA) microfluidic devices have gained prominence in various biomedical and analytical applications due to their exceptional material properties and compatibility with cell culture systems. However, the presence of residual uncrosslinked PEGDA monomers and photoinitiators within these devices can lead to cytotoxicity concerns, potentially compromising cell viability and experimental results. In this study, we present an innovative approach to reduce cytotoxicity associated with PEGDA microfluidic devices by implementing an isopropanol (IPA) washing method.Our investigation involves thoroughly characterizing the cytotoxicity of untreated PEGDA microfluidic devices and comparing it with devices subjected to the IPA washing procedure. We systematically assess cytotoxicity using cell viability assays and cell proliferation studies to quantify the impact of residual cytotoxic compounds on cells cultured within the microfluidic channels. Our results demonstrate that IPA washing significantly reduces the cytotoxic effects of PEGDA microfluidic devices, leading to improved cell viability and overall biocompatibility.Furthermore, we elucidate the mechanisms behind the reduction in cytotoxicity, shedding light on the role of IPA in effectively removing unreacted PEGDA and photoinitiators. This study provides valuable insights into the optimization of PEGDA microfluidic device fabrication processes, enhancing their biocompatibility and usability for various biological and biomedical applications.In summary, our research highlights the importance of addressing cytotoxicity concerns associated with PEGDA microfluidic devices and offers a practical solution through the implementation of an IPA washing method, ultimately expanding the potential of these devices in diverse scientific and clinical applications.

Lake Bonneville's Legacy: Unraveling Genetic Drift and Diversity Loss in West Desert Bonneville Cutthroat Trout

March 21, 2024 12:00 AM
Authors: Tanner Van Orden, Dennis Shiozawa, Peter Searle, Ana Kokkonen, Paul Evans. Mentors: Paul Evans. Insitution: Brigham Young University. The Bonneville cutthroat trout (Onchorynchus clarki utah) is the only trout species native to the Northern and Southern Snake ranges in Eastern Nevada. Streams on the east side of both ranges flowed into Lake Bonneville 12,000 – 15,000 years ago when Lake Bonneville was at its maximum. As Lake Bonneville retreated, Bonneville cutthroat trout in these streams were left isolated. To better understand how Bonneville cutthroat trout in the study area are coping with a changing climate, we Investigated the genetic diversity of Bonneville cutthroat in the Northern and Southern Snake ranges and compared them to historic samples. We found highly differentiated cutthroat trout populations in close proximity to each other and a genetic diversity loss of up to 86.3% in the last 12 years.

Is this you? Foucauldian ethics in multifactor authentication

March 21, 2024 12:00 AM
Authors: Maren Archibald. Mentors: Jared Colton. Insitution: Utah State University. Two-factor authentication was in use as early as 1994 with a patent belonging to telecommunications company Ericsson, which described the tech as “an authentication unit which is separate from preexisting systems." Today, multifactor authentication (MFA) requires a user to prove their identity by way of their knowledge, through a password or one-time code; their possession, through a physical key; or their inherence, through biometrics like a fingerprint or an eye scan. The secure sign-in method has proliferated in recent years, but while significant time has been dedicated to refining it, little if any research has been done in relation to its ethical implications. In his scholarship on discipline, Michel Foucault writes of “examination that places individuals in a field of surveillance.” MFA is one such method of surveillance — various forms track users’ location data, common usage and login hours, and biometric information. In my presentation, I will apply Foucauldian ethics to show how MFA is uniquely situated among other forms of societal documentation because of its purpose. The very data that depersonalizes users into numbers is also meant to be so precise and personal that it is the only way to believe users are who they say they are. And beyond MFA’s treatment of individual users looms the widespread collapse of a distinction between privacy and security. These values are not incompatible, but organizations have implemented MFA in a way that requires users to sacrifice privacy in order to gain security. My research will show how MFA aggrandizes the power differential between users and tech giants and threatens the ability to simultaneously maintain privacy and digital identity.

Construction Techniques in Ancient Fremont Pithouses

March 21, 2024 12:00 AM
Authors: Ellie Martin. Mentors: Mike Searcy. Insitution: Brigham Young University. As part of the 2023 Hinckley Mounds excavation, BYU Field School participants excavated a sizable amount of beam-impressed adobe and burnt wooden beams from the partially excavated pit house. This research proposal will focus on these two types of artifacts and what can be learned about the Fremont people through their study. Specifically, in this research project I will attempt to answer the question of what specific types of wood and adobe were used, and how they were used together to build the Fremont pithouse. To do this, the charcoal will be sent in for wood testing to get dates and tree type. I will also test the beam-impressed adobe to understand the type of clay used to make the hardened adobe. Finally, I will study the beam impressions in the adobe to find the average diameter of the beams used in the pit houses, any outliers in the diameters, and analyze any visible angles on corner pieces to put together a picture of how the beams and adobe came together to form the skeleton of the pit house structure. This research has the potential to reveal much about the Fremont people that lived at Hinckley Mounds, and help us to better understand their living conditions. The research that I conduct will potentially generate data about the age and type of wood used in their living structures, the diameter of the wooden beams they used, and the angles of the corners of the structure. At the conclusion of this research we aim to gain a better understanding of how the Fremont people constructed their dwellings, from the materials to their methodologies. I anticipate finding that the trees they used were locally sourced and date to a similar period of the other dates that we have from the Hinckley Mounds site, between 700–1300 CE. The pithouse will likely prove to be sub-rectangular, similar to Structure 1 at the Hinckley Mounds site, although any angles that can be found in the adobe impressions could give more precise data. All of this new data may serve to provide more insight into how the Fremont lived and chose to operate in their society.

Trace Metal Concentrations of Various Land Use Types Surrounding Utah Lake

March 21, 2024 12:00 AM
Authors: Alex Montgomery, Mason Gordon. Mentors: Eddy Cadet. Insitution: Utah Valley University. TRACE METAL CONCENTRATIONS OF VARIOUS LAND USE TYPES SURROUNDING UTAH LAKEMason Gordon, Alex MontgomeryKeywords: Trace metals, Soil, Land use, Utah LakeUtah Lake has a history of anthropogenic impacts that have resulted in the accumulation of trace metals (TMs) in the sediments of this region. Previous studies have evaluated the water and saturated soils, but have not provided a complete picture of the human impact on upland soils. Some pollutants may be contained in unsaturated soils and never enter the water due to the inherent soil characteristics and chemical properties of the TMs. Elevated concentrations of TMs in the environment pose hazards to the ecosystem and local residents. These impacts can be better understood by evaluating TMs in unsaturated upland soils. In this study a comparison of TMs in saturated wetland and unsaturated upland sediments, as related to anthropogenic sources, was completed. This study analyzed the types and concentrations of TMs to understand their mobility throughout the ecosystem. 52 core samples were collected from the saturated and unsaturated soils in eight sites. These sites represent recently developed areas (New Dev), more established areas (Mid Dev- those that have been developed over five years), recreational areas, mining areas, industrial areas, agricultural areas, and a wastewater treatment plant site. The soil samples were dried, ground, sieved, acid digested, and analyzed in the ICP-OES for TM (As, Pb, Cu, Cr, Cd, and Zn) content. Preliminary results show that TM concentrations in upland sediments were higher than those in wetland sediments. Cr levels in industry and Mid Dev are 43.0 ppm and 47 ppm, respectively. Saturated sediments at the same sites revealed Cr levels of 23 ppm and 21 ppm, respectively. This indicates that TMs are contained in unsaturated sediments. Of the observed land use types, Mid Dev is the most impacted, having the highest elevations of TM levels on average. Cd concentrations exceeded background levels in both New Dev (.97 ppm) and Mid Dev (.83 ppm). This is indicative of anthropogenic impact, as developed areas have higher TM content. The statements of this study will provide information to regulatory authorities in order to create policy to improve human health.

Documenting the Flora of Utah Valley Using Student-written Treatments

March 21, 2024 12:00 AM
Authors: Gideon Bowes. Mentors: Michael Rotter. Insitution: Utah Valley University. Correctly identifying plants in the field can be a daunting task. Making an accurate ID typically requires one to understand a complex lexicon of terminology, possess a level of inherent taxonomic understanding, and have the know-how to traverse complex dichotomous keys and species descriptions. This process often necessitates an array of resources, including large hardbound texts, online references, and stand-alone glossaries of terms. In order to better meet the demands of beginning botanists, we created the Field Manual of the Utah Valley Flora Project. This project includes students taking botany classes at Utah Valley University and tasks them with creating botanical treatments to the plants growing in the Utah County vicinity. The students’ treatments are curated with the objective of providing descriptions that are usable by their peers. Here, we present an example of how treatments are composed using the mustard family (Brassicaceae) and data regarding the efficacy of this project with regard to course learning objectives. The goal of this project is to simplify the identification process by producing a student-written, comprehensive text that is user-friendly and accessible to the beginning botany student.

Another Look at Underlying Mortality Model Used in Life Insurance Industry

March 21, 2024 12:00 AM
Authors: Benjamin Furniss, Britton Borget, John Sanders. Mentors: Patrick Ling. Insitution: Utah Valley University. Mortality model is the underlying model used by life actuaries to price life policies, set reserve amounts, and compute policy values. A mortality model investigates how mortality rates evolve over time. Current insurance law in many states (including Utah) suggest the use of Scale AA (or a similar model) in projecting future mortality rates, which is a special case of autoregression time series model. This model is flawed because it is built on the assumption that (1) there is no ARCH effect in the central death rates data, and (2) there is no unit root in the time series of mortality index. These assumptions are questionable. No wonder why state insurance laws (including Utah state insurance law) are recently revised in recognition of discrepancy between model predicted mortality rates and actual mortality rates. Recent published literatures indicate that the second assumption is questionable, as some statistical tests suggest that there is some near unit root in the mortality model. In this talk we want to argue that ARCH effect is present in the mortality data, so there is need to adopt a time series model that incorporates heteroskedasticity in the mortality data. We will later propose a GARCH model for better predicting future mortality rates – a key task life actuaries conduct, for it is important for life actuaries to predict what will happen over the next few decades of policy term.

HEIGHTS IN THE abc CONJECTURE. AN UNDERGRADUATE APPROACH.

March 21, 2024 12:00 AM
Authors: Brantson Yeaman. Mentors: Machiel van Frankenhuijsen. Insitution: Utah Valley University. There has been considerable curiosity at the graduate and postgraduate level in regards to heights, that is, heights in their relation to Diophantine geometry. One application of heights is in the $abc$ conjecture, which remains highly mysterious. Often, the only height undergraduates encounter is the traditional absolute value. This talk seeks to define the height for use in investigating the $abc$ conjecture and connect it at a level that undergraduates with little experience with number theory may approach. It will introduce the idea of a $p$-adic norm of a number, a projective point, and a view that lends itself to both a simple idea of distance, and yet has an analogue in the Hamiltonian numbers.

Inexpensive Optical Power Meter

March 21, 2024 12:00 AM
Authors: John Walton, Dallin Durfee. Mentors: Dallin Durfee. Insitution: Utah Valley University. We are developing an inexpensive optical power meter to measure lasers in our lab. In the device the signal from a photodiode is directed through a transimpedance amplifier to provide a voltage proportional to the power of the incident light. We will use an Arduino and a 16 bit ADC to collect the voltage measurement. The Arduino will also control a switch, allowing us to change the gain of the transimpedance amplifier for measurements over a larger range of powers. The Arduino will be programmed with the responsivity curve of the photodiode so that the correct power can be calculated for a given wavelength.

Asexual Aviators: Transcriptome Profile of the Life Stages of a Parthenogenic Mayfly

March 21, 2024 12:00 AM
Authors: Avery Larsen, Heath Ogden. Mentors: Heath Ogden. Insitution: Utah Valley University. Mayflies, also known as Ephemeroptera, are members of the anciently derived infraclass known as Paleoptera, the first group of insects that evolved the ability to fly (Gillott, 2005). Distinct developmental characteristics of the life stages of the mayfly are the reason that ephemeroptera are of particular interest. The life stages are; the egg, nymphs, subimago, and imago. The aquatic nymph stage does not have wings but instead has leaf-shaped gills that can be used to help propel the insect through its environment (Eastham, 1936). Centroptilum triangulifer will be used for RNA extractions to study the development of both wings and gills. Objectives for this research are 1). Elucidate, describe, capture, and record distinguishing characteristics of the different instars of Centroptilum triangulifer. 2) Identify key instars integral to gill and wing development in Centroptilum triangulifer. 3) Perform 10 RNA extractions. 4) Use Qubit technology to ensure ≥ 20 ng/μL RNA concentration per ≥ 10 μL. 5) samples will be packaged in thermo-stable shipping boxes and sent to Novogene where samples will be tested once more for quality control. 7) Once the quality is confirmed, samples will be processed using poly-A enrichment and then sequenced using high-throughput Illumina sequencing. Data will be tested for quality control and then sent to Ogden labs. 8). Using an in-house bioinformatics workflow, RNA data will be checked for quality, trimmed, and aligned, before RNA data is first trimmed, and then aligned to Ogden labs transcriptome reference genome. After alignment, the number of reads per gene, or hit counts, are calculated and compared. 9) RNA results of different instars will be compared to each other as well as NCBI databases using the Basic Local Alignment Search Tool.

Accelerated Rates of Evolution in hymenopteran DSCAM genes

March 21, 2024 12:00 AM
Authors: Remington R Motte. Mentors: Carl Hjelmen. Insitution: Utah Valley University. Down Syndrome Cell Adhesion Molecule (DSCAM, an immunoglobulin protein) is a protein that is important for neuronal development, especially in invertebrates. While DSCAM is present in other organisms, it has gone through multiple independent duplication events in arthropods. This has resulted in four to six DSCAM exon clusters with thousands of isoforms throughout arthropoda. Due to DSCAM’s role in brain development, I investigated DSCAM1 evolution and its relationship to the evolution of sociality in arthropods. I defined eusocial as having a common nest, cooperative brood care, reproductive castes and generational overlap. Any species that exhibited one or some of these traits I categorized as sub-social, and any that didn’t show these traits were categorized as solitary. Using Bayesian phylogenetic techniques, I compared DSCAM1 phylogenies to mitochondrion phylogenies and found higher rates of evolution in the DSCAM gene within Hymenoptera. The same increased rates were not found with other eusocial species. I then mapped sociality status onto phylogenies to visualize patterns of evolution and estimate ancestral condition of sociality for common ancestors. Increased taxon sampling within cockroaches and termites (Blattodea), as well as including ambrosia beetles (Austroplatypus incompertus), aphids (Pemphigus spyrothecae) and thrips (Kladothrips) could elucidate the relationship between DSCAM evolution and sociality in arthropods.

Altered Estrus Cycles in POMC-deficient Mice

March 21, 2024 12:00 AM
Authors: Nathan Anderson. Mentors: Zoe Thompson. Insitution: Utah Valley University. The pro-opiomelanocortin (POMC) gene is expressed in the hypothalamus and the pituitary gland. The products of this gene are important for food intake and energy expenditure. Mutations in the POMC gene can lead to obesity, excess hunger, and infertility. We are studying a mouse model of POMC deficiency in order to better understand what causes the infertility. In this experiment, we want to study the estrus cycle of mice who have the POMC mutation. The estrus cycle is similar to the menstrual cycle in humans. Specifically, the estrus cycle can be divided into four different stages: estrus, metestrus, diestrus, and proestrus stages. To determine which stage of the cycle the mice are in, we analyze the cells that are present in a vaginal smear, which is collected by a pipet smear technique, and then can be viewed under a microscope. This technique consists of filling a pipet with 0.9% saline solution and flushing this into the vagina of the mice. The cells lining the vagina will be washed off and then collected back into the pipet. These cells will then be added to a slide where they will be looked under a microscope and analyzed based on the number of cornified epithelial cells, leukocytes, and nucleated cells that are present. We have taken pictures of these smears and are analyzing them to determine what stage of the cycle the specific mouse is in at that time. Data was collected over a several week period. Since the POMC gene is absent in the mice being studied, we expect the estrus cycles in the mice to be abnormal. Based on our results, we will continue our research into looking at the specific hormone levels of the mice.

Caffeine's Cognitive Edge: Investigating Memory Enhancement during Non-Optimal Times of the Day

March 21, 2024 12:00 AM
Authors: Greg Avalos. Mentors: Sierra Warner. Insitution: Southern Utah University. The primary objective of this study is to explore caffeine's impact on explicit memory, particularly during the early morning when cognitive performance tends to be at its lowest. We aim to investigate how caffeine modulates attention, memory recall, and consolidation, shedding light on the less-understood psychological effects of caffeine in a real-world context. Participants will be assigned either the caffeine condition or the noncaffeine condition and will be tested on their explicit memory recall using the Craft 21 story recall test. Expected results are that those in the caffeine condition will score higher on the Craft 21 story recall test compared to those who didn't drink caffeine before the study.

Family quality of life partially predicts parenting stress differences between parents with and without children with autism.

March 21, 2024 12:00 AM
Authors: Megara Jensen, Matthew Hatch, Brock Hughes, Madeline Gillies, Spencer Myres, Maddie Blackham, Autumn Gould, Kate Leigh, Nate Lieshman. Mentors: Rebecca A. Lundwall. Insitution: Brigham Young University. Parenting is stressful. Of all children with special needs, parents of children on the autism spectrum report spending the most time at educational, medical, psychological, and behavioral appointments. They also have significant financial expenses. Therefore, we set out to determine if parents on the autism spectrum are more stressed than parents of neurotypical children. We recruited participants using fliers and posts on social media autism support groups and on bulletin boards across campus. The parents (all mothers) had children between 8- and 32-months-old. For this analysis, we divided families into the ASD-sib group if the toddler had an older sibling with autism and the neurotypical group if they had no first-, second-, or third-degree relatives with autism. We asked them to complete surveys using Qualtrics regarding Family Quality of Life, Parenting Daily Hassles, and Parenting Stress measures. Twenty-three parents completed all surveys (n = 7 in the ASD group and n = 16 in the neurotypical group). We performed an ANCOVA with Parenting Stress as the outcome variable, presence of ASD in the family as the grouping variable, and Daily Hassles and Family Quality of Life as predictors. Age was entered as a control variable. We found a trend for the significance for Family Quality of Life predicting Parent Stress (F [17, 23] = 3.80, p = .083, eta2 = .17). No other variables showed a trend for significance. This suggests that parents of children on the autism spectrum are indeed more stressed. Although we need to collect more data, this motivates us in a larger study we are conducting regarding the effects of mindfulness on parental stress levels.

Development of the midwestern blot technique for elucidation of pigment-binding proteins

March 21, 2024 12:00 AM
Authors: Tessa C Black, Craig D Thulin. Mentors: Craig Thulin. Insitution: Utah Valley University. Monarch butterflies (Danaus plexippus) are a distinctive and beloved species due to their unique wing coloration. In 2022, UVU student Kyri Forman and Dr. Craig Thulin identified seven pigments in monarch butterfly wings, three of which have not yet been identified in any other organism. The novel pigments are modified versions of xanthommatin, and their discovery implies the existence of presently unknown enzymes which catalyze the pigments’ biosynthesis. The western blot and its modified technique, the far western blot, are useful tools for identifying protein-protein interactions using antibodies and bait-proteins, respectively. To identify xanthommatin-binding proteins present in monarch butterfly pupae, we are developing a new blotting technique which we are calling the midwestern blot. This technique uses pigment molecules to identify the presence of pigment-binding proteins within a mixture. The midwestern blot technique will be validated is being validated using cytochrome C, hemoglobin, and myoglobin, three heme-binding proteins of known molecular weight and structure. Once validated, we will use the midwestern blot to identify xanthommatin-binding proteins extracted from monarch butterfly pupae. The midwestern blot will help promote future investigations into pigment-binding proteins, including the enzyme responsible for the novel pigments identified in monarch butterfly wings.

Who Split the Cheese? Search for Paucilactobacillus wasatchensis in a Local Creamery

March 21, 2024 12:00 AM
Authors: Brooklyn Matthews, Andrew Rowley, Michael Salas, Shaun Kader. Mentors: Michele Culumber, Daniel Clark. Insitution: Weber State University. Paucilactobacillus wasatchensis causes significant product loss in the dairy industry by causing gas build-up in packaging, and slit-crack defects in the cheese. By invitation, we tested a cheese manufacturing plant in northern Utah for Plb. wasatchensis in their creamery based on reports of puffed packages of aged cheese. We were allowed to swab several locations in the production area where contamination with Plb. wasatchensis was possible. Swabbed areas included the areas exposed to milk before and after pasteurization, floor drains, the cheese vat, curd cutters, cheddaring table, and packaging environment. Samples of raw milk, fresh curds, and gassy cheese were also collected. Swabs taken from surface samples were vortexed in 1 mL sterile saline solution. The solution was diluted and plated on deMan, Rogosa, and Sharpe agar with 1% ribose (MRS+R) agar. All agar plates were incubated anaerobically for 1 week. Enrichment cultures for Pbl. wasatchensis were also made by inoculating 0.1 mL of the saline rinse from each swab in MRS+R broth, and incubated for 2 days. After growth, were diluted and plated on MRS+R agar. After 1 week of incubation, we selected small (~1 mm), slow-growing colonies that were morphologically similar to Pbl. wasatchensis. The 16S rRNA gene and N-acetyltrasferase gene were amplified using PCR primers specific for Pbl. wasatchensis. Positive Pbl. wasatchensis isolates were found on the handle of the cheese cutter and in the final cheese product showing a gas defect. Our finding suggests that Pbl. wasatchensis is a potential contaminant in the creamery, and though only found in one location, may exist throughout the facility at unculturable levels. Further studies will use molecular techniques to identify Pbl. wasatchensis throughout the facility to identify bacterial DNA instead of live cells, which may more accurately represent the presence of this fastidious organism.

Ecological Dynamics of the Hypersaline Great Salt Lake North Arm

March 21, 2024 12:00 AM
Authors: Amanda Lee, Alex Phillips, Bonnie Baxter, Amy Schmid. Mentors: Bonnie Baxter. Insitution: Westminster College. The Great Salt Lake is home to a massive variety of microorganisms and insights into these populations will aid in understanding the ecosystem. The Union Pacific Railroad’s causeway has divided the lake into two distinct sections and over time caused the North Arm to increase in salinity over the South Arm and gain its hallmark pink color due to restricted freshwater input. Terminal rivers flow into the South Arm while the North Arm’s water evaporates, leaving behind accumulating salty brine, and the two very different waters have nurtured two accordingly distinctive ecosystems. Breaches periodically opened in the causeway allow the waters to mix and provide opportunity for the microbial communities of the two arms to intermingle. In a collaboration between the Great Salt Lake Institute at Westminster University and the Schmid Lab at Duke University, this project focuses on procuring a picture of the North Arm’s microbial diversity and the extent that it adapts to new environments over the four seasons, as well as what happens to the microbes and their nutrient exchange at that breach mixing point. Halophilic archaea and algae comprise the majority of biodiversity in the lake, and we hypothesize that those taxonomic compositions of the North Arm are stable over the different seasons, due to their extremophilic nature. Every season, we have collected water samples from the causeway, breach, and Spiral Jetty to grow and isolate bacterial colonies for DNA extraction and imaging. We also collect biofilm on filters to analyze RNA gene expression and metabolite usage as the environment changes. Over nearly two years of sampling, the hypothesis has been supported and we are seeing stability in community compositions in comparison to the less-salty South Arm. We are still interpreting results from the mixed breach samples, but thus far the microbes appear to be similar to those in the North Arm.

Transdermal antiseptic products as a method to decrease skin bioburden prior to surgery

March 21, 2024 12:00 AM
Authors: Kiersten Gardner, Hannah Duffy, Abbey Blair, Nicholas Ashton, Porter Stulce, Dustin Williams. Mentors: Dustin Williams. Insitution: University of Utah. Transdermal antiseptic products as a method to decrease skin bioburden prior to surgeryKiersten Gardner(1,2), Hannah Duffy(1,2), Abbey Blair(2), Nicholas Ashton(2), Porter Stulce1(2), Dustin Williams(1,2,3,4)1 Department of Biomedical Engineering, University of Utah, Salt Lake City, UT2 Department of Orthopaedics, University of Utah, Salt Lake City, UT3 Department of Pathology, University of Utah, Salt Lake City, UT4 Department of Physical Medicine and Rehabilitation, Uniformed Services University, Bethesda MDIntroduction: Preoperative skin preparation (PSP) kits are used before surgery to prevent surgical site infection (SSI). These kits consist of alternating scrubs of alcohol and Chlorohexidine Gluconate (CHG) or Povidone Iodine (PVP-I). Transdermal antiseptic products like Ioban, Tegaderm, and Surgiclear are also used clinically to eradicate skin bacteria by releasing antiseptic over time. Despite these precautions, infections often occur, usually stemming from a patient’s endogenous skin flora. The bacteria causing SSI reside deep in dermal sweat glands and hair follicles, untouched by traditional PSP. To eliminate these bacteria, antiseptics must diffuse deeper into the skin at concentrations above the minimum inhibitory concentration (MIC). We screened FDA-approved topical antiseptic products using a modified Kirby Bauer Assay to assess bacterial kill over time. We hypothesized that extended use of topical antiseptic products prior to surgery would kill deep-dwelling skin bacteria. We tested the extended use of these products on pig backs in conjunction with a CHG PSP to evaluate remaining bioburden. Methods:We determined the minimum inhibitory concentrations (MIC) for CHG and PVP-I against three common skin bacterial strains. We then took 6 mm biopsy punches of Tegaderm, Surgiclear, and Ioban and placed them on fresh bacterial lawns daily for 3 days. We measured the zones of inhibition (ZOI). Lastly, we applied the products to the backs of 4 Yorkshire pigs (n=4 products per test). After 48 h, we removed the products, performed a PSP- CHG scrub, and excised skin sections underneath the products. We homogenized the skin and quantified colony forming units (CFU)/g tissue. We analyzed the outcomes statistically using a mixed effects linear regression to determine significance.Results:The average MIC values for CHG and PVP-I were 2 and 2,000 ug/mL for Staphylococcus aureus, respectively. The average 24 h ZOIs for S. aureus were 7.9, 22.4, and 10.1 mm for Ioban, Tegaderm, and Surgiclear, respectively. The average log10 reduction for the CHG PSP, Ioban, Tegaderm, and Sugiclear were 1.87 ± 0.232, 1.65 ± 0.192, 1.69 ± 0.222, and 1.77 ± 0.316 CFU/cm2, respectively. The p values between the CHG PSP and Ioban, Tegarderm, and Surgiclear were 0.491, 0.572, and 0.746, respectively. Discussion:The benchtop data indicated adequate antiseptic diffusion and kill. In the porcine model, however; the presence of any of the products resulted in a statistically insignificant log reduction. Extended use of FDA-approved transdermal antiseptic products does not appear to decrease the skin’s bioburden. It appears that the products are not releasing high enough concentrations of the antiseptic to kill bacteria.

Artificial black holes: are they a threat to humanity?

March 21, 2024 12:00 AM
Authors: Tate Thomas. Mentors: Alexander M Panin. Insitution: Utah Valley University. We wanted to see if accidentally creating mini black holes in high energy particle collisions posed a real threat to humanity. To do this, we calculated some properties of such a black hole, such as its life span, radius, density, and minimum energy required. We found that it is unlikely to exist, let alone destroy the planet. Furthermore, we calculated what would happen if it were to exist, finding that it would move through the Earth with little resistance and with a small amount of Earth matter absorbed. Depending on initial velocity, the black either quickly escapes Earth or would settle orbiting it with the orbit part of which passes via Earth. It is interesting that in a simplified model of Earth as of a sphere of uniform density, the inner part of the orbit of black hole is also elliptical (as the outer is) but not Keplerian - with Earth center not at the focus but at the center of another ellipse. In the case of small initial velocity when entire orbit is inside Earth, the period of such inner orbit is constant regardless of birth location and initial velocity of black hole. The goal of this presentation is to discuss the results of our calculations and to explore potential applications to our understanding of interaction of mini black holes with ordinary atomic matter.

Quantifying Approximation Errors in the Flash Thermal Diffusivity Measurement Technique Using High Fidelity Simulations

March 21, 2024 12:00 AM
Authors: Tage T Burnett, Jakob G Bates, Christopher R Dillon, Matthew R Jones. Mentors: Christopher R Dillon. Insitution: Brigham Young University. In recent years, modeling and simulation have become more prominent in solving heat transfer problems. The accuracy and predictive power of heat transfer simulations is limited by the quality of the thermal properties used within the model. Thus, one method for improving computational accuracy is measuring thermal properties more precisely. Additionally, increased precision of thermal properties benefits other aspects of engineering including design and analysis. This research focuses on quantifying approximation errors in the widely adopted flash method for measuring thermal diffusivity. The flash method leverages several approximations to make it simple and easy to use; however, these approximations do not reflect reality and introduce measurement errors. Understanding these errors is critical for developing high-precision thermal diffusivity measurement techniques.In the flash method, the top surface of a small, cylindrical disc of material is subjected to a short pulse from a laser or flash lamp and the time-dependent temperature at the opposite surface is recorded. The thermal diffusivity is calculated using those temperature measurements in combination with a mathematical model. The accuracy of the flash method depends upon the accuracy of the mathematical model. One common mathematical model is the Parker Model. This model assumes that all of the energy from the pulse is deposited in an infinitesimally thin layer at the surface of the material and negligible heat is lost to the environment. These assumptions simplify the model, making it easy to use, but introduce errors into the measured thermal diffusivity.Computational methods can quantify these inaccuracies. Factors including heat lost to the environment, the temporal profile of the laser pulse, and the spatial distribution of the deposited energy can be incorporated into heat transfer simulations. Higher fidelity mathematical models can also be developed to account for these complexities. This project includes these and other factors to make simulations as realistic as possible. Various mathematical models, such as the Parker Model and higher fidelity models, are then used to calculate the thermal diffusivity from the resulting time-dependent temperature profiles and their measurements are compared to the simulated material’s true thermal diffusivity. Repeating this process for several material types will allow the precision of the models to be analyzed for each case. This analysis will be summarized at the conclusion of this project, providing a framework for developing more precise thermal diffusivity measurement techniques.

Subtle Force Communication for Intuitive Human-Robot Co-manipulation

March 21, 2024 12:00 AM
Authors: Samuel Charles. Mentors: Marc Killpack. Insitution: Brigham Young University. Robots have an incredible potential to help humans in extreme or dangerous situations due to their significant and consistent durability, strength, endurance, replaceability, etc. However, humans and robots move very differently, leading to difficulties working intuitively with a robot partner when completing a task such as lifting a heavy object. We recently conducted studies in which human subjects moved a 60-lb table to several different positions in a room; we recorded force and torque data, along with many other aspects of the movement. In these studies of human-human co-manipulation, we noticed a trend during particularly difficult maneuvers; when lifting the table to high positions or acute angles, subjects switched their hand holds on the table’s handles. This was likely an easier method of holding the table, but it may have also communicated placement, stability, understanding, and strength to the other partner, leading to a smoother and more intuitive movement and experience overall. If this is the case, this data could be used to help a co-manipulation robot both effectively understand the subtle commands in human movement and intuitively communicate needed movement to the human partner. This is particularly useful in emergencies like natural disaster sites and war zones, in which immediate help is needed, but there is no time to troubleshoot an unclear or unintuitive robot.

Exploring the Differences Among Attachment Styles with Cognitive Appraisal and Emotional Suppression

March 21, 2024 12:00 AM
Authors: Rebekah Hakala, Moroni Black. Mentors: Todd Spencer. Insitution: Utah Valley University. Over the years countless studies have delved into the theory of attachment due to its influence in the field of child development. Attachment theory is the idea that the sensitivity of parents or caregivers to a child’s bids for attention affect how the child will bond in relationships (Bretherton, 1992; Wilson-Ali et al., 2019). The style of attachment that a child has can affect them long-term and may influence other relational and developmental characteristics (Kurth, 2013). Due to the influence of attachment, our study investigates the relationship it has with cognitive reappraisal. Cognitive reappraisal is the ability to regulate one’s emotional states and the idea that a change in these thoughts is necessary to change negative emotions (Troy et al., 2017; Clark, 2022). With its ability to process and regulate emotion, cognitive reappraisal is another influential characteristic to the human psyche. Our results and analysis of the relationship between attachment theory and cognitive reappraisal come from a quantitative survey.The purpose of the present study is to examine the relationship among attachment styles and Cognitive Reappraisal. Our sample consists of 411 married individuals. Participants completed The Relationship Questionnaire (RQ; Bartholomew & Horowitz, 1991) and Emotion Regulation Questionnaire (ERQ; Gross & John, 2003). Results of the one way Anova were significant F (3,407) = 3.36, p=<.01. There was a significant difference in levels of cognitive reappraisal among attachment styles. Secure attachment (M=29.95, SD = 6.41), fearful attachment (M=27.27, SD = 5.92), preoccupied (M=27.99, SD = 7.27), and dismissing (M = 28.78, SD = 7.54). Results of the Bonferroni Post-hoc analysis indicated a significantly higher levels of cognitive reappraisal than attachment styles (p.<.001). Results provide empirical support that secure attachment styles tend to be beneficial for cognitive reappraisal.

Ransomware Resilience and Ethical Dilemmas: A Comprehensive Review of Threat Landscape, Impact, and Mitigation Strategies

March 21, 2024 12:00 AM
Authors: Asmaa Alsharif. Mentors: Sayeed Sajal. Insitution: Utah Valley University. Ransomware is malicious software which restricts users from accessing their computer and personal data through encryption. Ransomware attacks target a variety of victims including individuals, organizations, businesses, and governments. Attackers take control of this sensitive data and hold it hostage until a ransom is paid. Whether or not ransom is paid, the attackers’ damage can be irreparable. It includes but is not limited to exposing personal information, identity theft, blackmail, permanent deletion or corruption of valuable data, and Distributed Denial-of-service (DDos). Moreover, the financial implications of ransomware attacks are substantial. Victims face not only the direct costs of ransom payments, but also the expenses associated with system restoration and the potential fines for data protection violations. Furthermore, an ethical dilemma emerges from the response to extortion, as victims must grapple with the moral implications of potentially supporting criminal activities by paying ransoms to recover their data. This raises questions about the broader responsibility of stakeholders in cybersecurity and their duty to protect data privacy. The first ransomware attack emerged in 1989 and since then, ransomware attackers have persisted as a cybersecurity threat, striking at the core of data privacy. This paper delves into the multifaceted impact of ransomware incidents on data security, current ransomware techniques and strategies, and the ethical dilemmas they raise.Because ransomware attackers are constantly evolving their methods, it is important to routinely review the current literature to fully understand the tactics and techniques used by these cybercriminals. This research paper will review, analyze, and synthesize the existing literature about ransomware. It is crucial for individuals and organizations to be proactive, understand the nature of the threat, and take precautions to protect themselves and their data. This study encourages further research and discourse on the multifaceted impact of ransomware attacks on data privacy and the pursuit for effective countermeasures.

MRSA induced biofilm clearance by bacteriophage and antibiotic.

March 21, 2024 12:00 AM
Authors: Rainey Hughes, Avalon Marker, Elizabeth Bouwhuis, Yeshaswini Dudde, Bryan Dopp, Scot Carington, Jared Nelson. Mentors: Daniel Clark. Insitution: Weber State University. Antibiotic resistance is a pressing concern within the medical community as bacteria's resistance to antibiotics is escalating alongside the increased usage of antibiotics. According to the CDC, there are close to 2.8 million antibiotic resistant infections every year, with about 35,000 of them resulting in death. This issue has prompted antibiotic stewardship programs in clinics and hospitals to avoid adding to the list of resistant bacteria. Staphylococcus aureus, including the formidable methicillin-resistant S. aureus (MRSA) strain, poses a grave threat due to its antibiotic resistance. The challenges stemming from this resistance become even more formidable when these infecting bacteria assemble into biofilms. Biofilms are robust, adhesive layers composed of bacteria and their extracellular matrices of polysaccharides, proteins, and DNA. In clinical environments like hospitals, biofilms frequently develop on medical devices such as stents, catheters, and IV lines, as well as on metal and plastic surfaces of medical equipment. These biofilms exacerbate antibiotic treatments due to incomplete eradication; the most resilient bacteria persist after exposure. There is evidence indicating that bacteriophages, which are viruses that will a target particular species or strain of bacteria, have the ability to encode depolymerases. These depolymerases can identify biofilms, adhere to them, and subsequently break down extracellular polymeric substances. Furthermore, bacteriophages can produce lysins, which induce bacterial cell death through cellular lysis. These characteristics can potentially render the bacteria more susceptible to antibiotics. The use of bacteriophages can also be beneficial when it comes to the concern of opportunistic infections. Due to its selectivity to specific bacteria, it can attack the target hosts and leave the natural flora intact.In our research, we have induced biofilms in our bioreactor. With these biofilms we have been able to test different concentrations of multiple antibiotics, including Vancomycin, Oxacillin, and Carbenicillin in combination with phage K at different concentrations. Our research is aimed at showing a synergistic relationship between phage K and antibiotics, that will allow a subinhibitory concentration of both, in combination, to induce a complete kill and clearance. We have measured this by evaluating bacterial growth via absorbance measurements at 600nm in a Tecan plate reader. We have also measured biofilm clearance using the plate reader and measuring fluorescence at 630nm with a biofilm tablet assay. It was found that a subinhibitory concentration of antibiotic alone did not induce a complete kill and clearing, and that a subinhibitory concentration of phage alone did not induce a complete kill and clearing. However, once these concentrations were used in combination with each other, the complete clearing and killing of MRSA was achieved, and furthermore, was achieved with the antibiotic that the staphylococcus aureus is resistant to. Leading us to believe that we have found a renewed use for a currently ineffective defense mechanism.

Efficiently and Accurately Simulating Coupled Nonlinear Schrödinger Equations with Exponential Time Differencing and Fourier Spectral Methods

March 21, 2024 12:00 AM
Authors: Nate Lovett. Mentors: Harish Bhatt. Insitution: Utah Valley University. Coupled nonlinear Schrödinger equations (CNLSEs) are an extension of the nonlinear Schrödinger equation (NLSE) that applies to multiple interacting wave systems. They occur naturally in many physical systems, including nonlinear optics, multi-component Bose-Einstein condensates, and shallow water waves. Solitons, which are self-contained, localized wave packets that preserve their shape and speed during propagation, are a significant application of CNLSEs. Solitons are prevalent in nonlinear systems and play a critical role in long-distance information transmission in telecommunications. Despite their widespread use in various fields, solving CNLSEs analytically is challenging, and numerical approximations are necessary. However, solving CNLSEs numerically is a difficult task because of their high nonlinearity.To overcome this challenge, in this presentation, we will introduce, analyze, and implement an established fourth-order Exponential Time Differencing scheme in combination with the Fourier spectral method for simulating one-dimensional CNLSEs. In order to check the performance of this method in terms of accuracy, efficiency, and stability, we will present simulation results on CNLSEs. Our results will consider single, two, and four soliton interactions for homogeneous Neumann, homogeneous Dirichlet, and periodic boundary conditions. The numerical results will show that the proposed method is able to preserve energy and mass for a long time simulation in soliton interactions, as well as preserve the expected order of convergence for the proposed method.

Rotational Dynamics of Thrown Food Items and Their Impact on Canine Catching Success

March 21, 2024 12:00 AM
Authors: Caleb Slade, Evelyn Davis, Michael Orr, Brevin Bell. Mentors: Vinodh Chellamuthu. Insitution: Utah Tech University. Our study examines a viral video featuring a dog named Fritz who consistently fails to catch thrown food items. This research aims to determine whether Fritz's inability to catch is a result of his owner's throwing technique or Fritz's own inherent clumsiness. To investigate this, we employ a mathematical model based on kinematic differential equations to analyze the rotational dynamics of various geometrical shapes that simulate the thrown food items. By pinpointing moments in the projectile trajectory where the food items are most easily caught, we provide empirically-based recommendations aimed at improving Fritz's success rate.

Power Scaling a Nd:YVO4 Laser for Use as the Optical Drive of a Long Wave Infrared Optical Parametric Oscillator

March 21, 2024 12:00 AM
Authors: Alexander Gibb. Mentors: York Young. Insitution: Utah Valley University. Our research group’s long term goal is to build a student-constructed nonlinear optical system to create a tunable source of longwave infrared (LWIR) photons for probing molecules for early detection of disease in human tissue. I previously developed a diode laser system as the optical pump for a 5 Watt Nd:YVO4 laser which was reported at UCUR in Feb 2023. However, recent nonlinear optical calculations for our optical parametric oscillator (OPO) show that we will need the average laser power from that Nd:YVO4 laser to be in the 7-8 Watt range. My work to power scale the Nd:YVO4 laser is described in this presentation.

A Gyroscopic System for Magnified Coherent Diffraction Imaging

March 21, 2024 12:00 AM
Authors: Tyler Daynes, Jair Gonzalez, Jeremy Tait, Josh Jumper, Ellie Purcell, Tyler O'Loughlin, Vern Hart. Mentors: Vern Hart. Insitution: Utah Valley University. Coherent diffractive imaging is a common method for resolving small objects such as cells in order to determine their morphology. In it's essence it takes the diffraction pattern of laser light attenuating through an object and computationally reconstructs them back into the image that the light goes through. On the downside resolution of these diffraction patterns resulting from CCD size and sensitivity can be poor resulting into a less than optimal reconstruction. We have set out to build a system that can magnify this diffraction pattern without distorting the original image of the pattern. We have done this by building our own rastering system that can magnify light on the far edges of our beam. It does this by taking images with a lens attached to the rastering camera. This also has a problem because when the camera moves our detected light quickly falls off the screen of the detector. To fix this we added a gyroscopic system to our camera and lens so that the incident light may hit the system asmuthally at every point on the raster. This in turn will provide a higher quality diffraction pattern for reconstructing in CDI.

Using Ecological Niche Modeling to better understand Pediomelum aromaticum, a threatened Utah plant

March 21, 2024 12:00 AM
Authors: Ian Eggleston, Ashley N Egan. Mentors: Ashley N Egan. Insitution: Utah Valley University. Ecological Niche Modeling (ENM) is a very useful technique that gives us insight into a species’ present and possible future ranges, habitats, and niches. ENM has applications within conservational biology as models can be used to understand the extent to which climate change may impact a species. Additionally, ENM can be useful for prospecting for and propagation of rare plant species. This project will use ENM to create predictive range models for a rare plant species, Pediomelum aromaticum, with the goal of defining an ecological niche, determining impact of climate change, and general conservation of P. aromaticum. Here, we will compare 19 bioclimatic variables using correlation analysis and ecological niche modeling to determine which are the most impactful on the range of P. aromaticum. Additionally, ENMs will be created using the MAXENT algorithm from historical and predicted future climate data. By comparing these models, we can hypothesize as to how climate change may impact P. aromaticum. Finally, models will be compared between predicted climate futures defined as shared socio-economic pathways or SSP. SSP models estimate the impacts of human interactions within the scope of environment, governments, and each other to estimate the impact of varying sets of human interactions as defined by modified global behaviors within humanity and the impacts on global climate change. Different ENM models will be created with different SSP climate models so that we can understand how actions taken by humanity right now may impact the critically imperiled species P. aromaticum.

Effect of Print Parameters on tensile and flexure strength of 3D printed plastic

March 21, 2024 12:00 AM
Authors: Baylee Schumacher, Ryley Horrocks, Divya Singh. Mentors: Divya Singh. Insitution: Utah Tech University. 3D printed plastics have gained immense popularity in the recent times given the direct integration with CAD based software platform as well as ease of manufacturing. In addition, use of 3D printed parts can be more economical and less labor intensive.However, the mechanical behavior of these plastics is not well defined. There are many factors that affect the integrity of 3D printed plastic – extrusion temperature, infill pattern and infill percentage being a few of them.In this work, authors have attempted to study the effect of these factors on the tensile and flexure (bending) strength of 3D printed plastics. Firstly, tensile and bending samples are prepared by varying on more of the following – extrusion temperature, infill patter and infill percentage. Further, the specimens are tested in a destructive manner for tensile and flexure strength following the standard tensile and three-point bending technique on a Materials Testing System. The results on the variation in the strength as a function of print parameters provides an insight on the importance of standardizing these parameters to maximize strength and minimize cost.Keywords: additive manufacturing, 3D printed plastic, tensile strength, flexure strength

Effect of a supernova explosion on the mechaincal and thermal stability of orbiting planets

March 21, 2024 12:00 AM
Authors: Alexander Panin, Benjamin Miera. Mentors: Alexander Panin. Insitution: Utah Valley University. Recent searches for extrasolar planets have brought a surprising discovery – almost any star seems to have a planetary system around it. We know that massive stars end their lives in a violent supernova explosion, during which an extremely large amount of energy (~3x10^46 J) is released from the star in a very short time. In this presentation, we analyze the effect of this explosion – primarily the impact of the neutrino flash, the gamma ray flash, and the expanding plasma shell - on the mechanical and thermal stability of an orbiting planet. Our calculations show that a planet's orbit can be significantly disrupted by the momentum of the exploding star shell (depending on the planet's mass and proximity to the host star), but the radiation pressure from the explosion has a much weaker effect. If a star loses too much mass to the expanding shell, any previously stable orbit will become unstable, causing the planets to escape. Additionally, we found that the gamma ray flash and the plasma shell incident on the planet can cause significant heating, while the neutrino flash would have virtually no effect. Also, if a star’s collapse is asymmetric then the star itself can leave the planetary system due to the momentum of the asymmetric neutrino radiation. The sequence of events during a supernova explosion and how they influence such a planet is discussed in the presentation.

The Effects of a High Carbohydrate vs. High Fat Pre-Fast Meal on Incretin Hormone Secretion: A Randomized Crossover Study

March 21, 2024 12:00 AM
Authors: Parker Graves, Landon Deru, Bruce Bailey. Mentors: Bruce Bailey. Insitution: Brigham Young University. Chronic illness such as strokes, heart disease and diabetes all rank among the leading causes of death in the United States. Recently, fasting has gained popularity as a means of preventing and treating chronic illness. PURPOSE: Fasting produces multiple beneficial physiological responses that have been shown to aid in chronic disease prevention, one of which is observed in relation to incretin hormones such as glucose-dependent insulin tropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1). These incretin hormones are released by the gut to augment the secretion of insulin to regulate postprandial glucose levels. During a fast, the decrease in incretin hormones, and resultant insulin levels can aid the body to regain insulin sensitivity. This can lead to more effective blood glucose management and chronic illness prevention. The purpose of the study was to determine the impact of an acute 24-hour fast started with either a high fat (HF) or high carbohydrate (HC) meal on plasma GIP and GLP-1. METHODS: Subjects were over the age of 55, had a BMI equal to or greater than 27, and had no diagnosed metabolic disorders or some other disqualifying medical issues. Using a randomized crossover design, each participant performed two 24-hour fasts. One fast beginning with a high carbohydrate meal and the other a high fat meal, both of equal calories. Venous blood draws were taken at 0, 1, 24, and 48 hours. RESULTS: GIP and GLP-1 (P < 0.001) were both elevated 1 hour after consuming the pre-fast meal in both conditions. In addition, both GIP (P = 0.0122) and GLP-1 (P = 0.0068) were higher in the high fat condition compared to the high carbohydrate condition at 1 hour. There were no significant differences between conditions for either GIP or GLP-1 at any other time point. CONCLUSION: As expected, both incretin hormones spiked postprandially. We did find that GIP and GLP-1 levels were significantly higher at 1-hour postprandial for the HF meal compared to the HC meal. This could give evidence to show how macronutrient levels can affect incretin secretion and alter sensitivity to insulin. However, the impact of the pre-fast meal on GLP-1 and GIP did not persist throughout the fast.

Investigating Gender Differences in Facial Expressiveness during Personal Narratives Using a Modified FACES Protocol

March 21, 2024 12:00 AM
Authors: Leila Moore, Marin Farnsworth. Mentors: Tyson Harmon. Insitution: Brigham Young University. Background:Facial expressions are crucial for understanding human emotions in communication. Coding and quantifying these expressions, however, have often been subjective, leading to issues with reliability and consistency (Kring and Sloan, 2007). To address this, we have modified the Facial Expression Coding System (FACES) protocol (Kring and Sloan, 2007), which traditionally relies on subjective interpretation. Our modified protocol employs a more objective approach by quantifying facial expressions based on the counting of facial units (e.g., eyes, corners of the mouth, eyebrows, and teeth). Each intensity rating corresponds to a certain number of facial units. For example, an expression involving one facial unit would receive an intensity rating of one whereas an expression involving two units would receive an intensity rating of two. Multiple studies have found that women are more emotionally expressive than men overall when considering gestures, body language, facial expressions, and tone of voice (Ashmore, 1990; Brody & Hall, 1993; Hall, 1984). Rather than focusing on overall emotional expressiveness, though, the present study aims to understand how gender impacts the production of emotional facial expressions specifically. Method:The proposed study aims to compare the frequency and intensity of facial expressions produced by men versus women when recounting personal narratives. Participants. Video footage from participants with and without aphasia producing personal narrative discourse will be obtained for equal numbers of male and female participants. Samples will include two personal narratives in response to prompts from the interviewer. The first prompt is to speak of an experience the participant has had with illness/injury/stroke, and the second prompt is to talk about an important event that has happened in their life. Procedure. A modified FACES protocol will be used to code facial expressions in videos obtained from AphasiaBank. The coding process is conducted with the audio muted to help eliminate distractions. The modified FACES protocol includes specific, operationalized criteria for what qualifies as a facial expression. This protocol does not include coding non-facial gestures or eye movements. However, we do take into account the narrowing and widening of eyes in addition to instances when the eyes are opened or closed with intention. A critical question we ask during coding is whether the facial expression conveys emotional content. We have established standards for intensity ratings and will outline how we arrived at these standards, as well as the distinctions between intensity ratings one, two, three, and four. The analysis process includes establishing a baseline expression for each participant and coding for 20 minutes before taking a break to maintain energy and productivity and to ensure accuracy in data collection. Additionally, secondary coding and a final pass for gestalt ratings are performed to ensure comprehensive analysis.Data Analysis. To address our research questions, we will conduct a comprehensive analysis, focusing on our participants' facial expressiveness during personal narrative storytelling. The analysis will involve quantifying the frequency of facial expressions exhibited by the participants. Additionally, we will consider the valence of these expressions, distinguishing between positive and negative emotional content. We will also assess the intensity of facial expressions by counting the number of facial units engaged during each expression. This examination of facial data will allow us to discern not only the prevalence of expressions but also emotional content and intensity, ultimately providing a more in-depth understanding of the gender differences in non-verbal communication during personal narratives.Anticipated Results:Our study aims to explore if there is a statistically significant difference in the quantity, valence, and intensity of facial expressions between men and women. By using a standardized approach for measuring emotional facial expression production, we hope to shed light on the nuances of non-verbal communication during personal narratives and contribute to a better understanding of gender differences in emotional expression. Consistent with previous research, we anticipate that females will produce more frequent and intense emotional facial expressions than males (Ashmore, 1990; Brody & Hall, 1993; Hall, 1984).References:Ashmore, R. D. (1990). Sex, gender, and the individual. In L. A. Pervin (Ed.), Handbook of personality: Theory and research (pp. 486-526). New York: Guilford Press.Brody, L. R., & Hall, J. A. (1993). Gender and emotion. In M. Lewis & J. M. Haviland (Eds.), Handbook of emotions (pp. 447-460). New York: Guilford Press.Hall, J. A. (1984). Nonverbal sex differences: Communication accuracy and expressive style. Baltimore: Johns Hopkins University Press.Kring, A. M., & Sloan, D. M. (2007). The facial expression coding system (FACES): Development, validation, and utility. Psychological Assessment, 19(2), 210–224. https://doi.org/10.1037/1040-3590.19.2.210.

Addressing Health Care Access Disparities: Bridging the Gap for a Healthier Society

March 21, 2024 12:00 AM
Authors: Jayden Peacock. Mentors: Jodi Corser. Insitution: Southern Utah University. Healthcare access disparities remain a persistent and pressing issue in the community, disproportionately affecting marginalized communities. These disparities include aspects such as geographic location, socioeconomic standing, and ethnicity, all of which can hinder individual and societal growth. Addressing these disparities is vital as it can improve the well-being of individuals and therefore society. Examining the nature of healthcare access disparities helps present a holistic approach that can help bridge the gap, providing a more equitable healthcare system. Healthcare disparities are deeply embedded in the network of social and structural inequalities, driven by a complex interplay of geographical, economic, and cultural factors. Such disparities create health gaps, where individuals from marginalized communities are disproportionately burdened by a lack of access to essential medical services. The historical neglect of underserved communities has contributed to the persistence of these disparities, necessitating a comprehensive and transformative approach to address the problem.Effectively combating healthcare access disparities includes implementing community involvement that empowers underserved communities, enacting healthcare policy reforms to address systemic barriers, and leveraging locum tenens to improve access to care in all areas. Addressing these aspects will lead to a more equitable and inclusive healthcare system, that results in improved health outcomes and well-being for all individuals. Addressing healthcare access disparities is essential for individual and community improvement. Through community engagement, healthcare reforms, and the use of locum tenens a comprehensive approach can be represented in regards to a more equitable and inclusive healthcare system.

The Cold, Hard Truth: Cryopreserved Tissue is Superior to FFPE Tissue in Molecular Analysis

March 21, 2024 12:00 AM
Authors: Ken Dixon, Jack Davis, DeLayney Anderson, Mackenzie Burr, Peyton Worley, Isaac Packer, Bridger Kearns, Jeffrey Okojie. Mentors: Jared Barrott. Insitution: Brigham Young University. IntroductionPersonalized cancer care requires molecular characterization of neoplasms. While the research community accepts frozen tissues as the gold standard analyte for molecular assays, the source of tissue for all testing of tumor tissue in clinical cancer care comes almost universally from formalin-fixed, paraffin-embedded tissue (FFPE). Specific to genomics assays, numerous studies have shown significant discordance in genetic information obtained from FFPE samples and cryopreserved samples. To explain the discordance between FFPE samples and cryopreserved samples, a head-to-head comparison between FFPE and cryopreserved tissues was performed to analyze the DNA yield, DNA purity, and DNA quality in terms of DNA length.MethodsHuman (n = 48) and murine tissues (n = 10) were processed by traditional formalin fixation and paraffin embedding or placed in cryovials containing HypoThermosol solution. 19 human-matched samples were included. These cryovials were cooled to -80°C slowly and stored in liquid nitrogen until the time of the study. DNA was extracted using the same protocol for both tissue types except that tissues embedded in paraffin were first dewaxed using a xylene substitute followed by a multistep rehydration protocol using ethanol and water. Samples were weighed and calibrated to have the same starting mass. After the column purification, samples were eluted in 20 µL and concentration and purity were measured on a Nanodrop. Purity was determined by calculating the 260/280 ratio. DNA fragment length was measured on an Agilent Fragment Microelectrophoresis Analyzer.ResultsGraded amounts of tumor tissue (5- >50 mg) were used to determine the lowest starting material needed to extract 40 ng/mg of DNA. The average for both sample types reached the minimal threshold of 40 ng/mg. However, 74% of FFPE specimens failed to meet the minimum 40 ng/mg, whereas only 21% were below the threshold in the cryopreserved samples (Figure 1). In the cryopreserved group, the average DNA yield was 222.1 ng/mg, whereas 52.8 ng/mg was obtained from FFPE tissue. For DNA purity in cryopreserved tissues, the 260/280 ratio range was 1.09-2.13 with a mean of 1.79. The 260/280 ratio range in FFPE tissues was 0.85-2.76 with a mean of 1.65 (Figure 2). The DNA Quality Number(DQN) is a measurement of DNA fragment length and the percentage that exceeds the threshold of 300 bp. For FFPE, the DQN was 4.4 compared to a DQN of 9.8 for the cryopreserved samples (Figure 3). Setting a higher threshold of DNA length to 40,000 bp and measuring the area under the curve (AUC), it was observed that cryopreserved samples were 9-fold higher in fragments greater than 40,000 bp (Figure 4).ConclusionsCryopreserved cancer tissue provides superior quality assurance measurements of DNA over FFFPE. Treatment decisions based on molecular results demand accuracy and validity. The pathology community should support efforts to cryopreserve cancer biospecimens in the clinical setting to provide valid molecular testing results. The automatic pickling of tumor specimens in formalin is no longer an acceptable default.

Differences in Ultrasound Elastography Measurements Of The Patellar Tendon Using Pad vs No Pad and Of Dominant vs Non Dominant Legs

March 21, 2024 12:00 AM
Authors: Ashley Allan, Mikayla Kimball, Noah Bezzant, Brent Feland, Josh Sponbeck. Mentors: Brent Feland. Insitution: Brigham Young University. BACKGROUND: Recent studies have shown that there are differences bilaterally in the cross sectional area of the patellar tendon for lead vs non lead extremities of athletes. Yet, little research can be found as to whether there is a difference that develops over one’s lifetime between the stiffness of the patellar tendons in the dominant vs non-dominant legs. Reliability has not yet been established for elastography in the patellar tendons, so we are continuously striving to gather more reliable data on shear-wave elastography of the patellar tendon. PURPOSE: The aim of this study was to assess whether there is a difference in the average patellar tendon stiffness as measured by ultrasound elastography using a pad vs no pad and differences between self reported non-dominant vs dominant knee of senior athletes over the age of 50. Dominance taken as reported in a modified KOOS (Knee Injury and Osteoarthritis Outcome Score) survey. METHODS: Data was collected from 15 active, senior aged volunteers at the Huntsman World Senior Games in St George, Utah, 2023. All subjects (mean age= 67.29 ± 6.26 yrs, height=175.44 ± 8.18 cm, weight=87.40 ± 12.21 kg) signed an approved consent and completed a modified KOOS survey. Following, they were seated on a table, with their backs against the wall directly behind them. They were seated so that their lower legs were hanging off of the table in a relaxed position. The patellar tendon was then imaged with a long axis view using ultrasound elastography. ANALYSIS: All data were analyzed using JMP ver16.2 with a repeated measured analysis of variance (ANOVA) to determine if differences existed between pad and no pad and between dominant and non- dominant legs. RESULTS AND CONCLUSION: There was a significant difference between (p=.0423) pad and no pad patellar tendon measurements, but no significant difference when comparing sides combined with pad and no pad, between dominant and non-dominant legs, although a trend for significance did exist, and we suspect that with more subjects analyzed, we will get more significance.

Assessing Eccentric Hamstring Strength in football Players: Muscle-Specific contributions

March 21, 2024 12:00 AM
Authors: Logan McMaster, Joshua Sponbeck, Malorie Wilwand, A. Wayne Johnson. Mentors: A Wayne Johnson. Insitution: Brigham Young University. In the dynamic game of professional football, where split-second decisions and explosive movements often determine the outcome, players train all year round for optimal athletic performance. Paramount to this performance are the hamstring muscles including the semimembranosus (SM), semitendinosus (ST), and both the long and short head of the biceps femoris (BFLH and BFSH). They play pivotal roles in sprints, agility, jumping, and overall lower-body strength. Because of the critical nature of the hamstrings during functional activity, this study sought to determine which of these three muscles contributes the most to eccentric hamstring strength. Recognizing the correlation between muscle volume and muscle strength, this study compared maximal eccentric hamstring strength with the proportion of each isolated hamstring muscle's volume relative to the total hamstring muscle group volume. Doing so would identify which specific muscle contributes the most to eccentric hamstring strength. This study incorporated 74 male, collegiate football players representing all positions on the team. Maximal strength was assessed through Nordic hamstring curls and overall muscle volume was quantified using magnetic resonance imaging (MRI). Our results show no correlation (R^2<0.2) between maximal force output and proportional muscle volume for the ST, and the BFSH. Additionally, the correlations for these muscles were not statistically significant (P>0.05). The SM muscles had a weak negative correlation (R^2= -0.25) with eccentric hamstring strength that was statistically significant (P = 0.03). The BFLH proportional volume was positively weakly correlated (R^2= 0.22) with eccentric hamstring strength. This correlation was statistically non-significant (P = 0.06). This information shows that no hamstring muscle contributes more to eccentric hamstring strength than any other. Knowledge of this can be invaluable for clinicians and researchers to design comprehensive strength training programs that enhance the collective strength of the entire hamstring muscle group, ultimately optimizing the performance of football players.

Staying Hydrated - A Comparative Analysis of Humectants in Human Cadaveric Tissue

March 21, 2024 12:00 AM
Authors: Rachel Prince, Joseph Monsen. Mentors: Jason Adams. Insitution: Brigham Young University. Humectants are an important class of compounds that attract and retain water within a cell. These substances are commonly used in skincare products to prevent the outer layers of the skin from drying out. Humectants also serve a similar purpose in tissue preservation and prevention of decomposition of cadaveric specimens. As there have been few comparative studies analyzing the effects of different chemicals on the preservation of cadaveric tissue, we designed an assay consisting of wet-dry analysis to compare the effects of four common humectants (2-phenoxy ethanol, glycerol, propylene glycol, and ethylene glycol) on water retention in various cadaveric tissues. These tissues include human skin, skeletal muscle, brain, liver, cardiac muscle, and lung tissue. In each experiment, the tissue was submerged in the humectant and then weighed before and after being placed in an incubator. From these values, we calculated the percent difference in tissue mass to determine the most effective humectant concentration for retaining moisture in the tissue. We first tested a concentration gradient for each humectant to determine the most effective concentrations for each humectant. Then we performed a second set of experiments to compare the ideal concentrations of the humectants under the same conditions in each tissue. In our preliminary experiments, we found that glycerol is the most effective in retaining moisture in several of these cadaveric tissues. As we finish our experimentation we plan to create a tissue library to provide an evidence-based standard for wetting solutions used in anatomy labs.

The effects of terminating a 24-hr fast with a low versus a high carbohydrate shake on hunger and appetite

March 21, 2024 12:00 AM
Authors: Katelynn Hales, Katya Hulse, Spencer Hawes. Mentors: Bruce Bailey Jr.. Insitution: Brigham Young University. There is relatively little research examining the effect of intermittent fasting on hunger and appetite. The existing literature suggests a complex interaction between hormonal signals, subjective hunger, and actual eating behavior. Development of effective and sustainable protocols rests on understanding these relationships. Dietary protocols that involve continuous energy restriction have been shown to increase appetite. As intermittent energy restriction results from fasting protocols, there is reason to suspect that fasting may similarly impact hunger and eating behavior. However, some research suggests that ketogenic or very low energy diets may decrease hunger, making it unclear what the effects of fasting may be. Levels of ghrelin, the primary hunger hormone, typically increase over the course of a fast, whereas satiety hormones, like leptin, decrease. However, these hormonal changes do not always align with ratings of subjective hunger. There is some evidence that the post-fast meal may play a role in hunger and appetite. Different macronutrients have been shown to impact hormones, hunger, and satiety differently. Our study aims to determine how ending a fast with a high or low carb shake will influence hunger and satiety hormones and subjective hunger.

Transcriptomic Analysis of B cell RNA-seq Data Reveals Novel Targets for Lupus Treatment

March 21, 2024 12:00 AM
Authors: Sehi Kim, Naomi Rapier-Sharman, Michael Told. Mentors: Brett Pickett. Insitution: Brigham Young University. Systemic Lupus Erythematosus is an autoimmune disease that produces autoantibodies affecting various body regions, including skin, joints, kidneys, brain, aerosol surfaces, blood vessels, etc., resulting in damaging organs and tissue. Patients commonly experience an elevated risk of bleeding or blood clotting, joint stiffness, pain, fatigue, and depression.Our study involved the collection of RNA seq data of B cells of both SLE patients and healthy people from the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) database. Subsequently, we employed the Automated Reproducible MOdular Workflow for preprocessing and differential analysis of RNA-seq data (ARMOR) workflow. The differentially expressed genes identified by ARMOR were then analyzed using SPIA (Signaling Pathway Impact Analysis) algorithm to find the pathways associated with lupus. We further utilized the Pathways2Targets algorithm to predict potential lupus treatments based on known protein-drug interactions.In our study on lupus patients, analysis using ARMOR, SPIA, and Pathways2Targets identified 10,000 differentially expressed genes and revealed their modulated pathways, providing insights into molecular cascades in lupus. Furthermore, we identified potential drug targets, finding the way for therapeutic interventions that ultimately led to the discovery of new drug treatments. We anticipate that our findings could be utilized for the benefit of lupus patients, further advancing personalized medicine strategies, holding promise for improving the quality of life for individuals grappling with this complex autoimmune disorder.