Fine Arts

Authors: Hailey Packard. Mentors: Brandon Ro. Insitution: Utah Valley University. In the vast landscape of architectural mediums, the key to capturing clients' attention and ensuring a comprehensive grasp of a proposed project lies in the choice of rendering methods. This research endeavors to pinpoint the most effective communication medium through an experiment employing various rendering techniques. Four renderings of a single exterior façade will be crafted, each utilizing diverse media methodologies. To convert the renderings into quantifiable data an innovative approach involves subjecting the renderings to AI-driven algorithms, predicting where the human eye is drawn to in the images in the first 3-5 seconds superseding the influence of cognitive bias, and aiming to identify which of the images inherently captures the most attention. The research results will be examined and the significance of differences between rendering methods addressed. This research delves into the implications for architects, exploring how these findings may influence presentation strategies, considering potential impact of passing fads, taking into account the alignment of media style with architectural subject matter, and noting whether the experiment requires diverse architectural styles for optimal effectiveness. The current hypothesis regarding these results is that the images with contrast and hierarchy in the composition, such as watercolor renderings, will outperform the other methods. The overarching objective of this research project is to discern the most effective medium for capturing the client's attention when presenting architectural projects. Due to modern advancements that increase our access to an abundance of knowledge and techniques, architects and designers must make informed choices about how they present their ideas. By comparing these mediums and formats through these methods, this research will attempt to identify the most effective strategy for engaging clients and enhancing their comprehension of projects which will also aid in contributing to a clearer understanding of visual communication in the architectural field.

Authors: Joseph Laudie. Mentors: Brandon Ro. Insitution: Utah Valley University. For an architect, choosing how simple and/or complex your designs are can be a difficult effort. It involves balancing various factors, including appearance, function, cost, time, context, etc. While one might state that a certain way is best, it can be difficult to know. For that purpose, this study will examine the question, “How does the perception of a space or structure change as it evolves from a simple to a more complex state?” The difference between these conditions may involve an increased presence of elements, such as motifs, ornaments, etc., gradually becoming more complex. These changes will be analyzed using eye-tracking software.In contemporary architecture, architects grapple with multifaceted considerations. They must combine the varied desires of clients, adapt to evolving technology's possibilities and constraints, prioritize sustainability and efficiency, adhere to budget limitations, stay attuned to cultural and aesthetic trends, meet functional programming requirements, and navigate site-specific constraints. All these factors influence the architect's decision-making process, guiding them toward either simplicity or complexity in their design approach.Because of the difficulty of this cumulative decision, the issue will be thoroughly examined to determine the true effect both simplistic and complex structures have on the everyday individual. This will be done by examining a single, 3-D-modeled room with progressively more complex states, involving more details such as windows, doors, and classical orders and elements. Photos of the respective conditions will be presented side by side and processed through eye-tracking software.It is expected that more complex spaces and conditions will draw attention more consistently. While the analyzed space will present varying states of detail, it is expected that the more complex and expressive setting will consistently demand attention. This conclusion will better inform future design decisions by architects to make spaces more appealing and conducive to the observer.

Authors: Sean Donner. Mentors: Brandon Ro. Insitution: Utah Valley University. A building’s function has historically been tied to its form. We see this in civic buildings, schools, churches, and homes. Many of those recognizable forms were abandoned with the modernist movement in the early twentieth century. Are these new forms still related to their functionality and can average people perceive a building’s functionality based on its form. Learning the opinions of the general public will enhance the architect’s ability to design for the people who will live and work in and around the buildings they design.This study aims to answer those questions by means of an online survey. To gain a wider demographic of survey takers, the survey is largely spread through social media where I have access to a wide variety of people across the globe and of differing generations and backgrounds. The survey has two types of questions. The first presents two pictures of buildings and asks the survey taker to tell which holds a specific function. The second question type presents a single building and four choices of building function. In both question types, all signage or other markings of building type were digitally removed from the photographs. The survey includes fourteen architectural functions ranging from single family homes to State Capitols. Each category includes traditional and modern examples. This variety in style will help to analyze whether there is a difference in the general public’s ability to perceive a building’s function if the building is traditionally designed or if it takes a more modern approach in design. Although this survey is still active, the expected results are as follows: Traditional forms are generally more easily discernable than modern designs. Some building forms are equally recognizable regardless of form. The stand outs from this list are schools and stores/shoppes.

Authors: Desiree Ritchie. Mentors: Brandon Ro. Insitution: Utah Valley University. Both music and classical architecture have a strong foundation in proportion. Further research shows that the same systems of proportions are found in both areas. Common chords found in music can be translated into proportions found in architecture. For example, the octave has the same proportion as a 2:1 ratio. While there is a clear relationship, the question remains: does understanding one help in understanding the other? Does understanding music make one a better architect? Can designers benefit from musical instruction? To answer these questions, a comparative survey was conducted asking participants to determine which visual proportion best matched the sound heard. These questions range from basic chords and rectangles to a more complex comparison of the Fibonacci sequence to the golden ratio. The survey also asks about participants' background in both music and architecture, as well as general demographic questions. The demographics of the current survey are limited to students and faculty attending BYU and Utah Valley University, but further studies will provide a more comprehensive result. To analyze the results, a comparison will be conducted on the percentage of individuals who were correctly able to recognize the same proportions visually and audibly. This will then be cross-examined with the demographics, comparing those who have a background in music and/or architecture to those who do not. The expected result is that those familiar with one of the previously mentioned fields will better recognize proportions in both music and architecture. If this is proven true, it will show that having a background knowledge in multiple fields will help to create a more well-rounded and capable individual. It will provide insight on how to become better in one’s chosen field.

Authors: Alexia Trapier. Mentors: Brandon Ro. Insitution: Utah Valley University. I am studying architectural column orders and how they are interpreted by the average person. I chose this because I want to learn how people are drawn to the orders when looking at them so I may better understand the orders themselves, and how the world perceives them without an in-depth knowledge or understanding of their composition. I will be doing a comparison of three column orders via eye-tracking software. These consist of the doric, ionic, and corinthian orders. First, I will use the eye-tracking software over an image of each column capital on its own, afterwards I will run it again with all three images side-by-side. This process will help us discover which column order is preferred by the human eye, and why. I believe by doing these two comparisons we will learn which column order will draw the eye of its viewers, and how in-depth someone might look at the detailing of the capitals. I anticipate that the corinthian order may draw the most attention due to the higher level of detailing this capital contains. As a designer it is important to learn and understand what the human experience and interaction is with a building's design. To understand what your viewers prefer and how it makes them feel is important in the world of architecture. An architect doesn’t design for themselves, they design for others of the world, and thus, it is important to understand why people enjoy a certain amount of detailing, or proportionality. Although I’ve sampled at a small scale, it shows that there’s room for expansion into other aspects of design. I hope that through this research we can better understand why the classical orders are important to have in the world of design today and how they impact design in our world today.

Authors: Eric Burton. Mentors: Brandon Ro. Insitution: Utah Valley University. Which approach creates more interesting and beautiful buildings: Those who utilize ‘form following function’, or ‘function following form’? When an architect designs only using form following function, the building can have an excess of the utilitarian, resulting in a building that is very useful but can suffer in its overall design aesthetic, lacking attention to beauty. When the opposite occurs, the building can be an incredible work of art, but perhaps suffer in its usage. For an architect to be successful, there needs to be a balance of both methods.In the field of architecture, many architects and designers follow the adage ‘form ever follows function’. While the purpose of this research project is not to discredit this philosophy, the goal is to prove that the other side of the architectural coin, so to speak, has value and that there are multiple philosophies to consider.The research methodology for this project was comparing the buildings of architects that utilize differing philosophies with various building types, and utilizing eye tracking software to help determine which philosophy is more engaging, and beautiful to the average person. The results show that function following form typically produces buildings that are considered more pleasing to the eye, when it comes to the building facades that are evaluated. Building with form in mind first will allow for the designs to better fit their perceived building typology. There is worth at looking into different perspectives. When one is approaching design, they should be willing to implement multiple methods to achieve the best version of their building. Function following form is one method that can help us attain a more beautiful building experience that will impact the world for generations to come.

Authors: Kevin Hart. Mentors: Brandon Ro. Insitution: Utah Valley University. Context, in the realm of contemporary architecture, holds a significant role. It encompasses the information that conveys an understanding of ideas, scenes, or objects. In a world marked by increasing individualism, the consideration of contextual elements in architectural design often takes a back seat. The Royal Ontario Museum serves as a notable case study. The addition of the Michael Lee-Chin Crystal, contrasting with the traditional backdrop, has ignited debates regarding its compatibility with the existing context. This brings forth a critical question: How does altering the contextual surroundings impact people's perceptions?Furthermore, would the public's perceptions differ if the Crystal were the original building, with the traditional structure serving as the addition? This study delves into the role of contextual factors in shaping the architectural experience. Through a comprehensive survey featuring diverse architectural styles situated in various contexts, my aim is to discern preferences: whether individuals lean toward statement buildings or designs that naturally integrate with their surroundings. My research hypothesis suggests that people derive greater enjoyment from buildings that seamlessly integrate with their contextual environment.The implications of these findings are of significant value to architects and designers. They provide insights to facilitate informed design decisions that not only promote community cohesion but also elevate the public's appreciation of architectural structures.

Authors: Jacob M Wendt. Mentors: Brandon Ro. Insitution: Utah Valley University. This research delves into the transformation of urbanization over the past century, triggered by the influence of modern architectural concepts, and the potential ramifications for mental health. The primary objective is to investigate the neurological effects of specific urban layouts, with a particular emphasis on safety, social interaction, and aesthetic appeal, to gain insight into the human mind's urban preferences. A survey will be designed to capture subjective preferences in which respondents choose one of two anonymous center streets that are subjectively safe and attractive. Participants will favor the traditional street layout over the contemporary one, suggesting a preference for design associated with enhanced safety, aesthetics, and social appeal, affirming the statement by Allen Jacobs that optimal streets have paramount desirability. In parallel, eye-tracking software is utilized to objectively analyze subconscious neurological responses to diverse urban layouts and elements, providing a more scientific perspective on human perceptions of urban environments by revealing more balanced heat maps, and signifying a focus on aesthetic elements and reduced attention to potential hazards. The combination of survey and eye-tracking data presents a comprehensive argument in favor of traditional urban planning principles and expected to underscore the prevalent preference for traditional urban designs over contemporary alternatives. In conclusion, this research sheds light on the enduring human desire for both safety and aesthetic beauty in urban environments. Anonymous survey data ranging from 50 to 100 participants, coupled with eye-tracking simulations indicating balanced heat maps, highlights the potential of traditional design to cultivate healthier and more harmonious communities. Future research may delve into physiological measures, such as heart rate monitoring, to further validate the stress-reducing benefits of traditional urban planning. The findings from this survey are expected to provide actionable insights that can inform urban planning decisions and strategies that foster improved mental wellness and community well-being.

Authors: Annalicia Loveridge, Maren Daynes, Noah Guzman. Mentors: Donna Fairbanks. Insitution: Utah Valley University. This project includes the planning and execution of a Carnegie Hall performance featuring the Wolverine Piano Trio. Annalicia Loveridge, Noah Guzman, and Maren Daynes were the first-place winners of the 2023 American Protégé International Piano and Strings Competition in the College Students and Professional Musicians category. The performance will showcase the first movement of the Brahms Piano Trio No. 3.We will rehearse extensively leading up to the performance to ensure flawless execution of the selected piece. We aim to create an immersive musical experience for the audience, transporting them to a world of melody and harmony. The performance will be held in the Weill Recital Hall of Carnegie Hall, known for its remarkable acoustics and grandeur. Performers such as Lang Lang, Yo-Yo Ma, and Joshua Bell have all started their careers at Carnegie Hall. The Carnegie Hall performance is a milestone achievement for us as students and an opportunity to showcase our exceptional talent and passion for classical music to a global audience. It is a once-in-a-lifetime experience that promises to leave a lasting impression on us and bolster our professional goals.

Authors: George Wells. Mentors: Timothy Francis. Insitution: Utah Tech University. Cast in the form of a first-movement, 17th century Sonata, this is an original composition that explores different chord progressions out of dissonance to resolution. This solo piece for piano is structured similar to works typical of the classical time period. Piano repertoire from the classical era feature repeated patterns, simple melodies, and contrasting moods. Atypical of piano repertoire from the classical era are characteristics such as chromaticism, common-tone and augmented chords, polyrhythms, and hemiolas. From a framework of the sonata form with simple patterns and melodies, I explored the mood and color each method of resolution brings to music composition, classical and contemporary. From this work we can come away with a better understanding of the abstract emotion and character naturally found in musical resolutions that have been codified through music theory.

Authors: Brianna Bastian. Mentors: Alexandra Giannell. Insitution: Utah Valley University. My understanding of language as a whole began to broaden when I took my first of many ASL classes almost a decade ago. It continued to deepen and grow when I spent a year and a half in an immersive Spanish-speaking program. Now as a university student studying both Speech Pathology and Art, I have found myself recognizing the validity of art as a language. Thus, my research and presentation explores the linguistics of the visual arts.

Authors: Kimberly Lewin. Mentors: Anne Francis Bayless. Insitution: Utah State University. I have been exploring the relationship between connection, disconnection, and control, and I presented my research on these topics to three composers who wrote a piece based off of their interpretation. At URCO, I will perform one of these compositions.

Authors: Madi Hancock. Mentors: Nathan Crane. Insitution: Brigham Young University. Binder Jetting (BJ), a type of additive manufacturing (3D printing), creates parts through a multi-layered process. Particles are bound together using tiny droplets of liquid binder. Binder jetting has advantages over other additive manufacturing methods including relatively low costs, fast build rates, and a variety of possible printable materials. However, porosity defects commonly seen in BJ printed parts limit the technology's usefulness in demanding industries. There are several proposed causes of these porosity defects, including poor powder compaction in printed areas, residual carbon from the binder, and powder rearrangement due to binder droplet impact. The relative importance of these factors is poorly understood. This study will compare observations of porosity in printed and unprinted regions of Stainless Steel 316 BJ samples to better understand the possible modes of porosity defects.

Authors: Abby Hoyal. Mentors: Kristen Arnold. Insitution: Weber State University. The United States is one of the most prominent locations involved in the exchange of children in human trafficking. There are very few outlets that take in recovered children help them receive the proper aftercare and help to gain an education to integrate them back into society. Research has shown that children learn most efficiently in spaces that are modular and flexible. In research conducted for habilitation centers for children, they discovered, “Planning flexibility and variability comfort children and parents, accessibility and emotionality for children visiting [these] centers.” (Kasper, Ilvitskaya, Petrova, Shulginova, 2019). It has also been found that learning levels are highest in spaces that allow the children to learn from their surroundings rather than by just the instruction alone. “An interior shall lead children to learn concepts from working with materials, rather than by direct instruction. [Interiors] should improve cognitive learning, promote independence, curiosity, decision-making, cooperation, persistence, creativity, and problem-solving.” (Manav, 2016). One of the key elements to properly educating children, as listed previously, is to promote independence. One of the ways that research has shown independence to be achieved through design is proper wayfinding elements should be implemented so that occupants do not have to rely on any other occupant to navigate the space. Researchers examined how different colors, light temperatures, and lighting brightness can provide a natural easiness to wayfinding for occupants. Results found, that “the use of cool colors and high brightness levels help people be spatially oriented.” (Hidayetoglu, Yildirim, Akalin, 2012). The Grove will be an Aftercare center that will provide refuge and educate child survivors of human trafficking in a modular environment that educates through the use of space and materials used, with an encouraging push for independence throughout the space due to proper wayfinding elements.

Authors: Roya Adham. Mentors: Kristen Arnold. Insitution: Weber State University. Given the frequency and severity of natural disasters and humanitarian crises, around the globe, the current refuge center at Salt Lake City faces critical challenges in providing sufficient support for its demand.Security and efficiency of indoor space are critical matters in designing a refuge center.the absence of confidentiality can lead to information leakage and compromise the security of the building. (Reinten, et al 2017). The room acoustics will impact the creation of positive communication and interaction for individuals who seek solitude and peace inside the building. Oasis Haven promised to serve as a haven to those in need, helping them to nurture and heal from their past traumas. According to recent research, organizing and laying out spaces, and putting directional signage and informational labels to guide individuals who don't know the language will effectively increase their satisfaction level with the space Jamshidi et al. (2020). Accessibility for all individuals in a space is an ultimate goal of the designers and we must ensure cohesive visual language and consistency throughout the entire design.additionally, it studied and proved the effects of color schemes on employees' task performance due to the healing effects of colors Öztürk, Yilmazer, and Ural (2012). color plays a significant role in creating a space that helps refugees feel more at home and provides a sense of belonging to them. certain colors may symbolize peace, warmth, or security in one culture, while they might have different connotations in another Öztürk, Yilmazer, and Ural (2012) we try to incorporate all those principles and get help from other elements such as natural and artificial light to create space that feels like a true home and provide a sense of stability and comfort to refugees during the times of transition and adjustment.

Authors: Roya Adham. Mentors: Kristen Arnold. Insitution: Weber State University. Given the frequency and severity of natural disasters and humanitarian crises, around the globe, the current refuge center at Salt Lake City faces critical challenges in providing sufficient support for its demand.Security and efficiency of indoor space are critical matters in designing a refuge center.the absence of confidentiality can lead to information leakage and compromise the security of the building. (Reinten, et al 2017). The room acoustics will impact the creation of positive communication and interaction for individuals who seek solitude and peace inside the building. Oasis Haven promised to serve as a haven to those in need, helping them to nurture and heal from their past traumas. According to recent research, organizing and laying out spaces, and putting directional signage and informational labels to guide individuals who don't know the language will effectively increase their satisfaction level with the space Jamshidi et al. (2020). Accessibility for all individuals in a space is an ultimate goal of the designers and we must ensure cohesive visual language and consistency throughout the entire design.additionally, it studied and proved the effects of color schemes on employees' task performance due to the healing effects of colors Öztürk, Yilmazer, and Ural (2012). color plays a significant role in creating a space that helps refugees feel more at home and provides a sense of belonging to them. certain colors may symbolize peace, warmth, or security in one culture, while they might have different connotations in another Öztürk, Yilmazer, and Ural (2012) we try to incorporate all those principles and get help from other elements such as natural and artificial light to create space that feels like a true home and provide a sense of stability and comfort to refugees during the times of transition and adjustment.

Authors: Andrea Tyler. Mentors: Kristen Arnold. Insitution: Weber State University. The golden thread that runs through this project is ART. The texture, color, shapes, movement, contrast and emphasis which exists in ART will be carried into the interiors. Through this adaptive reuse project, the interior emphasis will be on the historical architecture and will draw on the textures and details as the backdrop. By tapping into these details, the overall aesthetic will ground the visitor to the experience within the space. The ARTshare Project mission is to uplift artists, gather and unite community, and empower human beings through ART. According to research (Pilegaard, 2023) “exhibitions might be said to produce an atmosphere of its own, neither is detached from the architectural interiors’ existing atmosphere. Indeed, they are dependent on it.” Atmosphere, distance and proximity in museum spaces is how we experience space. Access to light, combined with color, improves alertness and affects the soul and spirit. Combinations of color and natural light by using different types of glass throughout the design will stimulate moods and will be incorporated into this environment. Each loop in the golden thread of this project will represent an area of commercial, hospitality, healthcare and residential. Baniyamin, 2020 says, “Researchers have shown that visual stimulation of nature, natural lighting, artwork, relaxing colours and therapeutic sound can greatly accelerate the therapeutic process”. The art therapy rooms place a high priority on privacy while providing a healing environment with light, biophilia and art. Just like artists rely on nature for inspiration, so will the residential condos. With access to views, air and light, the design will connect the resident with nature. The bistro is the air that the interior breaths, inhaling and exhaling, healing and connecting.

Authors: Morgan Watson. Mentors: Kristen Arnold. Insitution: Weber State University. In the United States, 1 child in every 26 seconds drops out of high school. Out of the 50 million children in America, that are school age, 15 million are unsupervised after school time. With children being at greatest risk between the hours of 3pm and 6pm, these statistics raise a huge red flag. This project’s purpose is to provide peace of mind to working parents, and success for children throughout their school experience and beyond. Research has proven that afterschool program environments are a successful tool used to positively shape and increase healthy behaviors and practices among children (Huang, 2013). School design can affect student behavior, development, and academic performance (Huang, 2013). A key factor in effective school environments is the appropriate use of color. Careful color application has proven to have positive effects on behavior and cognitive learning (Heliyon, 2022). In addition, children have a better school experience when given autonomy. Autonomy improves intrinsic motivation (Ford, 2016). Research suggests that wherever possible, design for autonomy be implemented as a way to support children’s growth and development (Sorensen, 2013). This can be applied through multi-purpose learning spaces and flexible classroom furnishings. Additional spaces for collaboration and creativity are needed to meet the needs of today’s students and hold their attention. Furthermore, autonomy in the classroom environment is supported by designated areas where children can express themselves and make the space their own. Potential areas for this include cubbies and classroom bulletin boards.

Authors: Harrison Denning, Spencer Thompson. Mentors: Jeff Hill. Insitution: Brigham Young University. Traditionally, tensegrity structures have been a subject of interest for their architectural beauty and high strength-to-weight advantage. The field of tensegrity research has since grown to include robots and more complex latticed structures. More recently, tensegrity has been used to accurately model many biological systems, such as joints and spines. Part of this modeling has involved trying to better estimate these biological systems utilizing bi-stable and multi-stiffness tensegrity structures. Our research takes a closer look at how to build and optimize bi-stable tensegrity structures with multiple stiffness modes. By optimizing tensegrity geometry or spring-cable connections between rigid members it is possible to significantly change the models’ overall equivalent stiffness between stable modes. Our research delves into how changes in the shape of rigid members create differences in overall structure geometry between stable modes and a change in stiffness between the two modes. We also discuss optimal spring cable connections and optimal individual spring constants to further increase stiffness differences between stable positions. Furtherance of this work will involve building larger and more robust models to be used on the body as wearable structures. The application of this research heads towards the development of wearable tensegrity braces with the ability to switch between higher or lower stiffnesses to cater to the needs of the wearer.

Authors: Mikayla Buckway. Mentors: Kristen Arnold. Insitution: Weber State University. Women represent half of the world’s population, and half of the worlds potential. Many of those women face hardships and issues with nowhere to turn for help. The adaptive reuse design build project was created in response to the non profit organization Haven and their need for a women’s center. The selected area and building are of historic beginnings and are centrally located for the best optimization of the center. The center is needed to have multiple different functions and uses. From safe temporary living spaces to counseling offices the main focus will be to cultivate healing, rejuvenation and learning in the space. Studies show that the built environment we chose to surround ourselves with impacts our wellbeing and can be the catalyst for a hastened or slowed recovery (Sternberg 2020). A range of different design elements will need to be incorporated in the space to ensure the goals of the facility are met. The use of nature, biophilia, color and acoustics in designing the physical environment has been shown to optimize health, wellbeing and performance of tenants. Utilizing these elements in the Haven women’s center will be a crucial part of creating the desired space. Using these positive stimuli will also assist in creating a less institutionalized space and more of a home for healing.

Authors: Samuel Weisler, Colton Korpi, Josh Lythgoe. Mentors: Aliki Milioti. Insitution: Utah Valley University. The Venice Project addresses the challenge of blending contemporary buildings into the well-established urban environment of Venice, a city well known for its rich architectural heritage. In a city with canals in the place of streets, where motorboats and gondolas are the main mode of transportation, seasonal flooding at high tide continues to become a greater environmental concern. The research centers on the documentation and preservation of these valued characteristics through an analytical and interpretative research approach. The primary focus is on aspects such as perception, harmony of open and enclosed space, and the seamless integration into the urban and environmental fabric.The unique design constraints were taken head on in creating the one of a kind ‘Ca’Meriggiare’, a luxury hotel that enriches Venice’s heritage. In lieu of fighting against them, the design embraces the environmental challenges posed by the periodic flooding of the city and transforms them into an integral part of the design. For instance, the flooding is harnessed to create a charming, arcaded entrance exclusive to hotel guests arriving by boat, providing a unique entry sequence unphased by rising or falling water levels.The expected result of the research is a successful fusion of a contemporary hotel with the rich historical context of the city. The innovative design allowed the periodic flooding to become an integral part of the hotel that added a charm and uniqueness to the guest experience. Ca’Meriggiare stands as a testament to the harmonious integration of historical preservation with environmental adaption that honors Venice’s heritage. This luxury hotel case study offers a holistic perspective on theoretical and design considerations, emphasizing the importance of integration within the environmental dimensions. Rather than viewed as an isolated instance, it provides an overarching framework for innovation that will apply to the evolution of contemporary architecture.

Authors: Tyler Peterson, Anna Jensen, Daniel Orr, Anton E Bowden. Mentors: Anton E Bowden. Insitution: Brigham Young University. Prior to approval for usage, spinal disc replacements undergo rigorous and costly tests to ensure that they will perform as designed once implanted. Wear testing, specifically, is one of the most important tests as any loose foreign particles can cause osteolysis of the surrounding tissue. Due to this, minimizing the wear experienced by a joint replacement is of extreme importance, however, wear testing is often done in latter stages of the design process as a functioning prototype is needed. These tests can be prohibitively expensive and adjustments to the prototype only add to the costs, thus a more accessible test to get an initial estimate of the wear is desirable. There are current methods employed to perform these preliminary test results, including the use of a Tribometer, which tests friction and wear. Benefits of using a tribometer are that it can control environmental factors that parallel the ISO testing standards, however, these devices cost thousands of dollars. In the present work, we designed a cost-effective preliminary testing apparatus that accurately recreates loadings that the implant will experience in flexion and extension, as well as lateral bending. The machine consists of a roller that can be loaded axially by compressing a spring to mimic realistic compressive loading conditions, and a motor that will move the roller to replicate spinal bending. The design incorporates a spring element to ensure that contact between the roller and the implant is preserved. Predictable, repeated motions achieved with this machine can provide early insights into the implant wear modes, which can facilitate iterative design improvements. Such knowledge early in the design process can save costs by reducing the need for major design changes in the late stages of product development.

Authors: Lydia Beazer, Trevor Carter, Audrey Christiansen. Mentors: Larry Howell. Insitution: Brigham Young University. Outreach is the process of deliberate engagement with a range of diverse communities. It is a vital adaptation in an increasingly digital world, acting as a vehicle to extend the impact of work done in research labs. Increased exposure can attract and inspire future engineers and lead to new opportunities for research. Previously, BYU’s CMR lab invested in consistent outreach projects and collaborated with prominent social media influencers, developing a seven-step plan to connect the public with academic research. Recently, these strategies were implemented in a new collaboration with influential YouTuber Mark Rober. In preparation for the anticipated increased visibility from this project, the lab organized a team dedicated to establishing a consistent and professional digital presence. For months, this team undertook preliminary steps that included updating outdated files, designing appealing and shareable makerspace content, and expanding the archive of publicly accessible resources. Having laid this groundwork, the lab was able to influence the impact of this high-profile collaboration, resulting in measurable increase in several metrics related to exposure and positive interaction with lab research.

Authors: Bronwyn Brown. Mentors: Ben Felix. Insitution: Utah Valley University. Architectural Analytique of the Thomas S. Monson Center Building with the Scamozzi Order at University of Utah

Authors: James Walker. Mentors: Pania Newell. Insitution: University of Utah. During the COVID-19 pandemic, the discussion of using fibrous porous materials in the context of face masks has gained significant relevance. These materials consist of networks of fibers that are intertwined through weaving, knitting, or bonding, creating a structure with interconnected pores that facilitate the transport of gasses and liquids. When a face mask is used, it is under tensile stresses that can greatly affect its longevity and behavior, and simulating the behavior of the fibers within the mask under this loading is essential in enhancing its robustness. Numerical analysis involving fibrous porous materials is challenging due to their inherent randomness and anisotropy, however. The models we use need to accurately represent the entire mask, which we achieve using a small cubic cell known as a representative volume element (RVE). In this study, we systematically investigate the role of fiber diameter, fiber cross sectional shape, and RVE size on the mechanical properties of various RVEs using a computational framework built on the finite element method. The RVEs themselves are idealistic, but useful networks of polypropylene fibers that are orthogonally intersected within cubic boundaries. Our results show that once an appropriate RVE size was determined with constant porosity between systems, the stiffness of the samples increases as the cross-sectional shape progresses from a triangle to a square, to a pentagon, etc., largely due to the increases in intersection volume between fibers. We also found that increasing the diameter serves to increase material stiffness. This project not only offers insights into designing more robust face masks but also provides novel tools that can be used for designing other fibrous porous materials.

Authors: Alberto Miranda, Samannoy Ghosh, Yong Lin Kong. Mentors: Yong Lin Kong. Insitution: University of Utah. Microscale robots can impart a broad range of functionalities in the biomedical domain that can be leveraged to address unmet clinical needs, including noninvasive surgery and targeted therapies. Conventional robot navigation methods typically involve specific gaits suited for certain environmental conditions. However, implementing the same conventional methods inside a human body is highly challenging. As the human body is a complex and dynamic environment, a microrobot must adapt to these complex and challenging environments to perform targeted studies. Previous research demonstrated an integration of an untethered, 3D-printed three-linked-sphere crawler with a model-free reinforcement algorithm. The work done with the theoretical Najafi–Golestanian three-linked-sphere mechanism was its first experimental integration with a reinforcement learning algorithm as a relatively simple and highly scalable self-learning robot that can navigate in unconfined and confined spaces. The progress presented in the current research is a direct continuation of the previous work on the 3-linked-sphere crawler. While the previous work focused on developing a proof of concept for adaptive gait learning for the crawler, the current work focuses more on the challenges of implementing the robot in a low Reynolds number fluid medium. Our current research hypothesizes that a self-learning autonomous system could demonstrate successful gait adaptation in a low Reynold’s flow environment. The design of our robot has been significantly improved to make it sustainable for extended use under viscous fluids. The research presented outlines the work that has been done to transition the robot from a crawler into a swimmer, the challenges that have been faced, and how they have been addressed. Successful implementation of this 3-sphere-swimmer will be a step forward in integrating machine learning tools into microswimmers for autonomous gait adaptation inside the human body.

Authors: Evelyn Fuentes, Thomas Keate, Christian Riordan. Mentors: Aaron Davis. Insitution: Utah Tech University. Studies predict that extreme weather events, due to climate change, are expected to increase in frequency and magnitude. Specifically, the flooding impacts from a hurricane may lead to the loss of necessary infrastructure, such as water treatment plants, leading to the loss of drinkable water. In response we, as a multidisciplinary team, have developed a purification device that is able to effectively filter water to allow communities and families, without available infrastructure, to receive drinkable water. We are testing different processes of filtration to find the most efficient and cheapest method. This process of filtration would be possible due to a foldable solar array that would power a pump to push water through a filtration system. The solar array would supplement other disaster relief options due to its ability to be used without constant supervision, and it would be capable of continuous, reliable use. This device would allow for the production of drinkable water in the event that water purification infrastructure was down, but grey water was available. The solar array and water purification device would be portable for fast deployment, with options of building a larger device, as part of a disaster relief preparedness package. If successful, this device has the potential to increase disaster preparedness and save lives through providing clean water.

Authors: Josh D Gubler, Connor D Olsen, Fredi R Mino, Mingchuan Cheng, Jacob A George. Mentors: Connor Olsen. Insitution: University of Utah. The long-term goal of this research is to investigate how lower sampling rates of electromyographic (EMG) signals affect the performance of classification and regression algorithms. EMG signals measure the electrical activity of muscle contractions. Myoelectric interfaces can classify or regress features generated from the EMG signal to control devices like prostheses, exoskeletons, robotic systems, or human-computer interfaces. Most of the power of the EMG signal is contained between 50 and 500 Hz, and most recording devices sample EMG at 1 kHz with a 5-15 Hz high-pass filter and a 375-500 Hz low-pass filter. As myoelectric devices become wireless and integrated with wearable technology, reducing the sampling rate can substantially reduce battery consumption and processing power. We sampled EMG data at 30 kHz from the forearms of three participants while they performed six gestures. We then downsampled to rates ranging between 50-1000 Hz and calculated various EMG features from the downsampled data. We found significant effects for both EMG feature and sampling rate on regression performance of a modified Kalman Filter (p < 0.05, two-way ANOVA). The mean-absolute-value and waveform-length EMG features performed significantly better at low frequencies (<250 Hz) in contrast to zero-crossing, slope-sign-change, and mean-frequency EMG features (p < .05, multiple pairwise comparisons). Sampling rate also had a significant impact on the classification accuracy of a k-nearest neighbors algorithm (p < 0.05, two-way ANOVA). However, sampling rate had no impact on classification accuracy for a continuous Convolutional Neural Network (CNN) (p > 0.05, two-way ANOVA). Future work will validate the effectiveness of this CNN as a control modality when using downsampled EMG from wearable sensors. If proficient control can be achieved from down sampled EMG, this could substantially improve battery life and make EMG a more practical biosensor for wearable devices.

Authors: Sophia Hessami. Mentors: Elizabeth Vargis. Insitution: Utah State University. Age-related macular degeneration (AMD) is the leading cause of blindness in developed countries. During later stages of AMD, immature blood vessels penetrate Bruch’s membrane and release fluid into the subretinal space. This process is referred to as choroidal neovascularization (CNV). Current in vitro models of retinal tissue are limited, so we propose a three-layered microfluidic model of the subretinal tissue, consisting of retinal pigment epithelium (RPE), Bruch’s membrane (BrM), and choroid. We have produced models of BrM using hagfish proteins that are more mimetic to the nonporous, proteinaceous BrM that is seen in vivo. Then, we fabricated a three-layered microfluidic device using the BrM models and polydimethylsiloxane (PDMS). Once the devices were assembled, porcine primary RPE were isolated, cultured, and characterized in the upper channel of the microfluidic device. Going forward, HUVECs will be cultured and characterized in the lower channel of the device. Then, primary RPE and HUVECs will be co-cultured and characterized within the device. The result will be a multilayered microfluidic device containing primary porcine RPE, hagfish protein BrM models, and human umbilical vein endothelial cell (HUVEC) choroid. It is expected that RPE protein secretions will diffuse through the BrM models and initiate interconnected vascular network formation in the endothelial cells. In the future, we will induce chemical hypoxia to turn this model into a diseased model of the subretina. We hypothesize that this in vitro model of the subretinal tissue will lead to a better understanding of the mechanisms of CNV initiation and progression in AMD.

Authors: Rui Jin, Lindsay C Rupp, Anna Busatto, Rob S MacLeod. Mentors: Rob S. MacLeod. Insitution: University of Utah. Introduction: The electrocardiogram is the most common tool to diagnose and assess cardiac conditions, such as rhythm abnormalities, myocardial ischemia, and heart failure. However, clinical diagnosis and management of heart disease are challenging due to the remote nature of body-surface electrocardiogram measurements, with a median accuracy of 67% among physicians. One approach to improve the accuracy of electrocardiography is to conduct mapping studies in which 10-100 catheter-based electrodes are inserted within the heart. The recorded signals provide more proximity and thus accuracy, but they also require specialized software to analyze, quantify, and visualize. We developed the Signal Processor for Electrogram and Electroanatomic Data (SPEED), a new, open-source, unified pipeline to facilitate effective signal processing and visualization of such cardiac-mapping signals.Materials and Methods: Our pipeline is based on two existing toolboxes, the Preprocessing Framework for Electrograms Intermittently Fiducialized from Experimental Recordings (PFEIFER) and OpenEP. PFEIFER is a toolset for sophisticated signal-processing of cardiac electrograms that allows the user to select semi-automatically fiducial markers, which are time points and intervals of interest within a heartbeat. OpenEP primarily accepts as input complete electroanatomic data, including both processed cardiac electrograms and spatial geometry; OpenEP also provides built-in functions for analyzing and visualizing cardiac electrograms, such as displaying potentials on the cardiac geometry. Since both software packages provide complementary workflows for managing electrograms, our goal was to integrate the two software packages and present it to the user as a new Graphic User Interface utilizing both applications simultaneously.Results: It was natural to develop SPEED in MATLAB as this is also the language used for both PFEIFER and OpenEP. The primary interface to SPEED incorporates a data-centric design such that the user can provide the electrogram and geometry files to be processed, and the algorithm automatically determines the applicable functions based on the input type. Since both PFEIFER and OpenEP can parse data into more interpretable open-source formats, the user can also export the processed data for further analysis in addition to visualizing and quantifying the data features. Through integrating both software packages, SPEED can support the following main functionalities: (1) in-depth filtering and processing of electrogram signals, (2) visualizing anatomic geometry and electrode locations, and (3) mapping three-dimensional potential and activation of cardiac electrophysiology.Discussion: SPEED offers the user a more thorough and unified workflow in the analysis of cardiac-mapping signals than either of its components. The user can utilize the functionalities of both PFEIFER and OpenEP simultaneously, allowing for a versatile and powerful processing pipeline. For instance, the user can extract key features from the recorded electrograms and visualize the location of the corresponding electrodes, a feature that was previously not possible. In addition, the open-source nature of the software packages allows the user to modify or expand the functions to better suit their individual needs. The software design of SPEED is still in the early stages; thus, as with most software, further development and user testing will follow to make the algorithms compatible with more data types and implement additional features. Conclusion: SPEED processes and displays the complex information in a clear and accessible way, allowing the user to perform subsequent interpretations and analyses more easily. SPEED can be used by research cardiologists to facilitate a more efficient workflow, as well as to improve the efficiency and accuracy of clinical diagnosis of heart diseases.

Authors: Chase Mortensen, Juhyeong Lee. Mentors: Juhyeong Lee. Insitution: Utah State University. Foldcore sandwich composites (FSCs) are constructed using multi-layered sheets folded in a desired pattern and placed between two thin face sheets. The choice of material geometric folding pattern provides a large design space to optimize the structural performance of FSCs. These composites are typically made of carbon fiber reinforced polymer (CFRP) composites, offering lightweight and high-energy-absorbing properties. This work aims to characterize the size effects of unit-cell foldcores by analyzing the influence of subscale foldcore models subjected to periodic boundary conditions under quasi-static compression. Three Miura-based unit-cell foldcore models were considered: (1) 1×1, (2) 1×2 (two 1×1 unit-cell foldcores connected in parallel), and (3) 2×1 (two 1×1 unit-cell foldcores connected perpendicularly). Through finite element modeling, three key findings were derived: 1) the finite element model closely replicated experimental results; 2) the application of periodic boundary conditions had an insignificant impact on subscale foldcore models. Third, inconsiderable variations in stress and damage were observed primarily along the foldcore creases when unit-cells were placed in parallel.

Authors: Alisa Dabb, David Britt, Elizabeth Vargis. Mentors: David Britt. Insitution: Utah State University. Cytomegalovirus (CMV) is the leading infectious cause of birth defects in the United States. CMV is typically treated with ganciclovir, an antiviral medicine that inhibits the virus. However, ganciclovir also inhibits the growth of neutrophils, a type of immune cell, which leaves the patient vulnerable to other viruses and diseases. To combat the toxic effects of ganciclovir, a subtherapeutic dose of ganciclovir can be used with the combinatorial treatment of quercetin and poloxamer 188 (P188) while maintaining the same level of antiviral activity. Quercetin is a hydrophobic natural flavonoid with antiviral properties that is found in many fruits and vegetables. P188 acts as the delivery vehicle for quercetin and is an FDA-approved polymer that targets the mitochondria in a cell. This study examines two delivery vehicles—P188 and Dimethyl Sulfoxide (DMSO) to optimize the combinatorial treatment of quercetin and ganciclovir.DMSO is a solvent for both polar and nonpolar compounds. DMSO is beneficial for cell growth at low concentrations. Additionally, DMSO successfully delivers hydrophobic quercetin to infected cells, although it does not target quercetin delivery like P188. Targeting the mitochondria, like P188, could be valuable because one mechanism of CMV infection occurs when the virus attacks the mitochondria in an infected cell. This study aims to understand if mitochondrial targeted delivery of quercetin better protects cells against CMV infection compared to non-targeted quercetin delivery.

Authors: Lukas Keller, Jixun Zhan, Zhen Zhang. Mentors: Jixun Zhan. Insitution: Utah State University. Curcumin is a common dietary supplement found naturally in the plant turmeric (Curcuma longa). Native to South Asia, the turmeric plant has been an important component in Indian and Chinese folk medicine. Curcumin has long been known to be an effective antioxidant and possesses anti-inflammatory properties. In today’s world, curcumin is a common nutraceutical and plays a part in the billion-dollar supplement industry. However, production and extraction of this compound is difficult and uses vast amounts of resources to cultivate. One solution to produce natural products like curcumin is the use of metabolic engineering to synthesize the product in another organism. The USU Metabolic Engineering Lab has developed a synthetic metabolic pathway to produce curcumin from an amino acid inside genetically transformed E. coli. The use of metabolic engineering techniques can produce larger quantities of the desired compound in greater quantities and purities while using a fraction of the land, water, and energy. To inform the use of these techniques, a predictive computational pathway was developed and is being validated with experimental results. An effective model can help researchers and businesses by allowing them to accurately predict curcumin yield and concentration during production.

Authors: Melissa Wiggins, Aaron Bigelow, Porter Ellis. Mentors: Ronald Sims. Insitution: Utah State University. Ensuring that the hydrophilic coating of Merit Medical’s Prelude IDeal trans-radial catheter is necessary for its biocompatibility and patient safety. The current method for testing the coating involves a test using Congo Red Dye. The Congo Red Dye does work, but the dye is toxic and all tested catheters must be discarded after testing. The Conge Red Dye test results in wasted catheters. A new method for testing the coating uses fluorescent particles. First, fluorescent particles are added to the hydrophilic coating. These fluorescent particles can be easily visualized on the catheter using UV light. Thus, the uncoated portions of the catheter can be visualized as well. The coated catheters are placed into a black box that ensures only the catheter is being seen. A line scan camera is used to take pictures of every side of the catheter as the catheter spins. Photos of the scanned catheter are then linked together, showing the entire circumference in one picture. The catheter is ultimately tested by analyzing the full picture to find any uncoated regions. By using software to analyze the full picture, the size of uncoated regions is determined with greater accuracy. This new method allows for tested catheters to be used after testing and does not involve any toxic chemicals.

Authors: Jacob Adams, Larry Catalasan. Mentors: Tianyi He. Insitution: Utah State University. Soft robotics is a field of robotics involving the controlled movement and manipulation of soft materials to fulfill tasks that standard robots cannot. In this project, we aim to create a soft robotic arm capable of movement by using a machine-learning algorithm to generate its subsequent moves. To fulfill this goal, the robotic arm is contained in a metal frame that has cameras monitoring its position. The camera feed is then processed through a machine-learning algorithm into instructions that can be used to pull various strings attached to the arm which will allow the arm to move. Currently, our team has finished building the frame/arm as well as software that can use cameras to map the position of the arm. The next steps in this project are to research and implement a machine-learning algorithm and write a program that can appropriately adjust stepper motors to pull the strings.

Authors: Ethan Layne, Tareq Hassan. Mentors: Juhyeong Lee. Insitution: Utah State University. A key design criterion for aerospace structural applications is specific mechanical property (i.e., mechanical property divided by the density of a material). Honeycomb sandwich panels which are commonly used in aerospace/aviation structural applications provide lightweight performance, however they have several drawbacks. They include (1) limited alteration of core geometric parameters, (2) few core material selections, and (3) a closed-cell core network. These limitations may be bypassed with 3D-printed lattice-core sandwich panels to provide customizable structural performance. This study investigates impact resistance of architectured sandwich panels designed with various core designs and infill densities. A series of 5~20J low-velocity impact (LVI) tests will be performed on 3D-printed ABS sandwich panels with honeycomb, gyroid, and triangle cores; with infill density varying from 5% to 15%. In this work, the effects of core geometry and corresponding infill density on LVI resistances will be studied to optimize the structural performance of 3D-printed ABS sandwich panels. The primary objective of this study is to characterize these novel sandwich structures with highly customizable 3D-printed complex cores, offering tailorable structure performance.

Authors: Cassandra DuBose Corry. Mentors: Elizabeth Vargis. Insitution: Utah State University. Age-related Macular Degeneration (AMD) is characterized by a blurring of the central vision and is one of the leading causes of vision loss in the United States. As a branch of the disease, exudative AMD is distinguished by retinal angiogenesis, when new blood vessels grow into the retina. Understanding retinal conditions that promote or discourage angiogenesis by using mathematical models can lead to improved understanding of disease progression and treatments. This discrete mathematical model presented here uses the theory of reinforced random walks to simulate the biological behavior of endothelial cells (ECs) as they leave a parent blood vessel and travel through the choroid and Bruch’s membrane towards the retinal pigment epithelial (RPE) layer. Cell behavior such as number of divisions and blood vessel coverage are analyzed for comparison to experimental observations. Pigment epithelium-derived factor (PEDF) is included and examined for its effect on the behavior of the ECs and its ability to prevent angiogenesis. This computational model provides novel insights into exudative AMD with parameters that can be adjusted to meet different needs.

Authors: Cassandra L Burdick, Caleb J Thomson, Troy N Tully, Jacob A George. Mentors: Caleb Thomson. Insitution: University of Utah. The long-term goal of this research is to decode fine motor intent from electromyography (EMG) of hemiparetic muscles. Stroke is the leading cause of disability in the United States, with 800,000 individuals experiencing a stroke each year. Eighty percent of stroke survivors experience hemiparesis. Severe hemiparesis immobilizes the arm, making it difficult to assess EMG control and motor function on traditional tasks. Here, we introduce a variant of the clinical Box and Blocks Test (BBT) of hand dexterity in virtual reality (VR) to assess fine motor function of EMG control in hemiparetic stroke patients with immobile arms. Our VR variant of the BBT allows individuals to control a VR hand to transfer VR blocks back and forth between two locations separated by a barrier. The VR hand can grasp, rotate, and translate based on EMG commands or other control signals received at 30 Hz via UDP communication. The forces exerted on the blocks and the location of the blocks and hand are logged to assess grasping precision, force regulation, and transfer rate. Multiple block sizes can also be used to assess dexterity with various grip apertures. The ability to assess EMG control in patients with hemiparesis can support the development of myoelectric orthoses. Practicing dexterous myoelectric control in VR may also help alleviate hemiparesis and expedite qualification for myoelectric orthoses.

Authors: Libby Heath, Kyle Cink, Thomas Petitjean, Ainsley Parkins, Sylvia Lee, Rodolfo S Probst, Matias Giglio, Baldomero Olivera, Macy Olivera. Mentors: Rodolfo S Probst. Insitution: University of Utah. Natural product-derived compounds occupy a wider chemical space than those synthetically obtained. Different invertebrate groups have emerged in recent times as a reliable source for novel pharmacological targets. A recent promising source for those compounds is marine gastropods. Despite their ubiquity, those organisms remain understudied, with the diversity of small marine gastropods (micromollusks of <1cm) in SE Asia–a hotspot for those mollusks–, still largely unexplored. In the Philippines, micromollusks are usually sampled by local fishermen with the traditional method known as lumun-lumun, involving placing fish nets in different water depths creating an “ecosystem” for mollusk’s larval settlement. The combination of bioprospecting importance and neglected systematic studies for SE Asian micromollusks press for the implementation of Operational Taxonomic Units (OTUs) and biodiversity cataloging of those organisms. To overcome this challenge, we turned to molecular identification of species collected from different islands in the Philippines. We tested whether short DNA fragments from lumun-lumun specimens collected in the last 10 years could be obtained by utilizing next-generation sequencing (NGS) with Oxford Nanopore Technologies. From a pool of 120 specimens, we obtained short DNA sequences (>450 bp) from 27 specimens. We merged our 27 barcodes with all available DNA barcode sequences from the Philippines’ Turridae included in the Barcode of Life Database (BOLD). Our phylogenetic analysis placed our 27 barcodes throughout the Turridae phylogeny. We were able to match three of our morphologically defined OTUs into valid Turridae taxa. One of our OTUs (“Lumun sp.42”) was recovered scattered across the Turridae phylogeny, matching three different genetic clusters of the hyperdiverse genus Gemmula. Therefore, our approach using NGS barcoding could be used in tandem with the current morphological classification. Finally, due to the presence of pharmacologically active compounds, which could be harnessed for various medical treatments, our results could serve as a foundation for helping identify novel pharmacological targets.

Authors: Sylvia Lee, Ainsley Parkins, Rodolfo S Probst. Mentors: Rodolfo S Probst. Insitution: University of Utah. Mutualisms between ants and plants are ubiquitous interactions and have been evolving for over 100 million years. Studies have been done on systems wherein the plants provide nutritional resources for ants, but the dynamics of mutualistic interactions in environments where host plants do not provide obvious nutritional rewards continue to remain elusive, raising questions about their co-evolutionary histories. In these circumstances, associated fauna, such as mealybugs and soft scales (Hemiptera: Coccoidea: Pseudococcidae and Coccidae), serve as intermediaries of the association, providing honeydew (enhanced plant sap) as a main food source to their host ants. However, the identity of the insects involved in these associations and their co-evolutionary history with ants is poorly understood. By focusing on samples collected in Costa Rica from 33 colonies of the Neotropical plant-ant genus Myrmelachista (Formicinae: Myrmelachistini), we sequenced DNA barcodes (cytochrome oxidase subunit 1 [CO1]) using Oxford Nanopore Technology and employed Next-Generation Sequencing (NGS) for both the Myrmelachista ants and their associated fauna. We aimed to identify their taxonomy, phylogenetic relationships and identify unique interactions between ants and their associated fauna. We recovered DNA barcodes for eleven Myrmelachista species and seven putative species of mealybugs (n=5) and soft scales (n=2). For the seven putative species of Coccoidea, our DNA barcodes, when compared to available sequences from the NCBI database, recovered an average of 85% match similarity. The closest matches associated our putative species to Old World Coccoidea taxa, reinforcing the need for taxonomic studies of this superfamily for the Neotropical region. Each putative species of soft scales was found uniquely associated with two Myrmelachista species (M. flavoguarea and M. cooperi). For all eleven Myrmelachista species, five were associated with two putative mealybug species. One putative mealybug species was associated with five different Myrmelachista species; one was uniquely associated with M. nigrocotea. Same associations were found between the associated fauna of M. flavocotea and M. lauropacifica; M. haberi and M. RSP027; M. plebecula, M. meganaranja, and M. osa, respectively. These results suggest that there may be unique associations between some Myrmelachista species and their Coccoidea associates, unconstrained by the ant phylogeny. Our findings also provide insights on Coccoidea taxonomy, emphasizing NGS DNA barcoding as a tool to illuminate how ant-plant associations might have evolved.

Authors: Sydney Larsen. Mentors: Joshua Steffen. Insitution: University of Utah. Pollen preserves are crucial to maintaining Apis mellifera’s hive health. This pollen provides bees with a source of protein, amino acids, lipids, and other nutrients that are vital for larvae development (Dharampal et. Al, 2019). In addition to providing these essential nutrients, bee collected pollen also provides the hive with an excellent source of external pollen-borne microorganisms. These microbes provide a variety of benefits including serving as a major dietary requirement for larvae, assisting in biochemical processes such as bee bread fermentation, and assisting in bee resistance to pathogens (Gilliam, 1997). Despite the importance that these organisms play on bee health, there is currently little research on how the microbial composition of bee foraged pollen changes throughout the foraging season in response to abiotic and biotic factors. Rapid advancements in DNA metabarcoding approaches and DNA sequencing technologies have made the assessment of hive level foraging patterns easily accessible. We attempt to leverage the benefits of these new approaches to characterize how microbial composition in pollen provisions changes in relation to shifts in foraging preferences. Here we describe general patterns plant foraging behaviors for two hives across a single foraging season. In addition, we describe the variability of microbial diversity associated with pollen across this same time frame. Our initial description plant and microbial communities present in pollen provisions suggest that pollinator foraging preferences play a significant role in pollen associated microbial communities. This will allow for further research into how the presence of specific beneficial microbes is affected by changes in climate, floral availability, pathogen presence, etc..ReferencesDharampal, P. S., Carlson, C., Currie, C. R., & Steffan, S. A. (2019). Pollen-borne microbes shape bee fitness. Proceedings of the Royal Society B, 286(1904), 20182894.Gilliam, M. (1997). Identification and roles of non-pathogenic microflora associated with honey bees. FEMS Microbiology Letters, 155(1), 1–10. https://doi.org/10.1016/s0378-1097(97)00337-6

Authors: My Nha Quyen Tran, Cindy Greaves. Mentors: Martin Horvath, Cindy Greaves. Insitution: University of Utah. I have been studying the DNA repair enzyme MutY found at the Lost City thermal field 3000 feet deep in the Atlantic Ocean with conditions similar to those that may have shaped the origin of life. With no sunlight and very little oxygen, life at the Lost City is driven by chemical transformations in rocks at extremely high levels of pH and temperature. DNA in all organisms strictly follows base pairing rules in which A pairs with T and G pairs with C. We all think of oxygen as beneficial, but it can cause damage to DNA. Oxidized guanine (OG) violates the base pairing rules and pairs with A. This causes mutations, which can lead to changes in the genetic information. The mutagenic potential of OG was a challenge that had to be solved early in the emergence of DNA-encoded life. The enzyme MutY functions in suppressing mutations and therefore protects us from developing cancer by finding and removing A from OG:A mispairs. In my effort to replicate conditions at the Lost City so as to explore how the DNA repair enzyme MutY evolved to function in this strange environment I need to purify the enzyme. I used DNA cloning to attach the MutY-encoding genes to a soluble bacterial protein called GST that hopefully will make it easier to purify the MutY proteins. Encouragingly, these fusion proteins expressed well in bacteria and appeared to be soluble. I am now exploring different conditions looking for optimal enzyme activity, which I predict to be different from what familiar bacteria can tolerate. Basic science research on how DNA repair enzymes adapted to the strange environment at the Lost City may inform future translational applications to treat and cure cancer and thus ensure a healthy society in Utah.

Authors: Nicholas Poll. Mentors: Moriel Zelikowsky. Insitution: University of Utah. Social interaction is one of the chief drivers of evolution and natural selection. Animal interactions shape the ecological world and have shaped the world’s evolutionary timeline. Of the many types of animal interactions, one of the most influential to behavior and development is aggression. Many animals' aggressive behaviors and mechanisms are so distinct that their origins can be traced back to specific selective pressures. These pressures range from reproductive competition to predator-prey interactions and have apparent effects on many species’ developments. There are many stressors that can increase aggressive tendencies found in mammals, yet it’s clear that the neural bases that connect these stressors and their behavioral responses are understudied. The targeted brain region in this project is the bed nucleus of the stria terminalis (BNST). The BNST is a neurochemically diverse node of the extended amygdala that has been implicated in mediating stress and sensory information. It anatomically acts as a relay station between a number of different structures including the hypothalamus and amygdala. The BNST has been implicated to have a role in stressor-induced aggressive behaviors; however, the neural mechanisms that connect this region and these behaviors are relatively understudied. Corticotropin-releasing Hormone (CRH) in mammals displays upregulation following the introduction of a stressor and has been found to mediate aggression and anxiety. Similarly, tachykinin-expressing neurons have been shown to have a role in mediating aggression in mammals. While CRH and tachykinin’s link to anxiety and aggression is well supported, it is not clear to what extent it has on BNST. This project aims to provide data to support the role that CRH and Tachykinin 2 (Tac2) in the BNST have in mediating aggression in mice through optogenetic stimulation.

Authors: G. Colton Gardner, Lynasi Gapelu, Sohom Mookherjee, Sihem Boudina, J. David Symons. Mentors: J. David Symons. Insitution: University of Utah. With the prevalence of cardiovascular disease increasing world wide and its precise mechanisms remaining elusive, the need for novel treatments is urgent. Autophagy is a conserved process whereby dysregulated intracellular proteins are escorted to the lysosome for recycling. Emerging evidence indicates that autophagy is important in maintaining endothelial cell (EC) proteostasis and function. My laboratory showed that autophagy repression in adult mice ECs produces arterial dysfunction, and indexes of autophagy are repressed in ECs of older mice and humans which display concurrent EC dysfunction. Sequestosome 1 (p62) tethers proteins and enables their entry into the autophagic pathway. The contribution from EC p62 to cardiovascular and metabolic function is unknown and experiments are needed to investigate this. Thus, p62 will be depleted from ECs of adult male and female mice (p62iEC-/-) via tamoxifen regimen, and results will be compared to animals wherein EC p62 is intact (CON). The efficacy and specificity of EC p62 depletion will be evaluated in ECs and vascular smooth muscle by qPCR and immunoblotting. Three adjacent 1 mm thoracic aorta sections will be obtained 14 days after the tamoxifen regimen. The central aorta section will be used to measure intrinsic mechanical stiffness by wire myography, with the other two used to find lumen diameter and intimal medial thickness. These measurements will help create Stress vs. Strain curves and the Elastic Modulus, which will be compared between the groups to show the differences caused by p62 depletion. We expect p62 depletion from ECs to increase intrinsic aortic stiffness.

Authors: Alexander Romano, Matthew Hesterman, Rachel Hill, Melodie Weller. Mentors: Melodie Weller. Insitution: University of Utah. Sjogren’s Disease (SjD) is an autoimmune disease diagnosed by symptoms of reduced tear and saliva excretion, accumulation of lymphocyte foci in the salivary glands, and the occurrence of antibodies against Ro (SSA), La (SSB), and nuclear proteins. The cause of SjD is unknown, though previous studies have detected the sequence and antigens of Hepatitis Delta Virus (HDV) in the minor salivary gland acinar, ductal, and adipose cells of patients with SjD; without the typical presence of a Hepatitis B coinfection. In this study, murine models were transduced with Adeno-associated virus containing expression cassettes for Luciferase (control), small, large, or a combination of both small and large HDV antigens to evaluate the impact of HDV antigen expression on salivary gland function and SjD autoimmune disease development. After a ten- or four-month period, the models were analyzed. Findings included a significant increase of inflammation for samples expressing both small and large HDV antigens, a significant increase of anti-SSA(La) antibodies in samples expressing the short HDV antigen, and a correlation between increased overall inflammation and decreased overall saliva flow. Performing qPCR methods verified the amount of HDV in the submandibular glands and Illumina sequencing portrayed an increase in glycolysis and beta oxidation metabolism in models with detectible HDV sequence. The models showed significantly increased IgM expression in the HDV exposed murine models, without significant change of other antibodies. Future plans include an ELISA diagnostic assay to verify the antibody levels and further sequencing analysis. With this information we can build a better picture of the direct mechanisms of HDV-mediated changes in salivary gland dysfunction and determine the extent that HDV can inducing systemic SjD symptoms.

Authors: Dallin Oyler, Ana Alfaro, Kailee Myxter, Ben Brooks, Amanda Brooks. Mentors: Jessica Pullan. Insitution: Southern Utah University. Nausea and vomiting are common complications that occur in 70% of pregnancies. Hyperemesis Gravidarum (HG) is the most severe form of these symptoms and is estimated to be prevalent in 0.3-2% of pregnancies. Due to the fetal and maternal morbidity associated with HG, identifying the cause and treatment options for these women is a critical task in obstetrics. Research regarding the etiology of HG has been fairly recent and is still ongoing, however, evidence had directed to a positive correlation between increased levels of the serum protein GDF15 and HG symptoms. We hypothesize that polymorphisms in both maternal and fetal DNA plays a role in the upregulated GDF-15 seen in mothers experiencing symptoms of HG during pregnancy. The DNA of 2 mothers and their corresponding children were sequenced and analyzed. The DNA was obtained through buccal swabs from the epithelial cells of the inner cheek, and then purified and ran through PCR. We employed 3 distinct primers that correspond to mutations in the genome that account for the elevated levels of circulating GDF-15 in the mother. As of current, the mother-daughter DNA is still under analysis for single-nucleotide polymorphisms, however recently published literature has suggested results similar to our hypothesis. M. Fejzo et al. shows that upregulated serum GDF-15 is primarily of fetal origin, and that maternal sensitivity to GDF15 increases the risk for developing HG. Additionally, the DNA coding variant GDF15 C211G was shown to elevate the risk of HG

Authors: Cate Bateman, Carson Veazie, . Sydney Sandall. Mentors: Matt Ogburn. Insitution: Southern Utah University. Western Utah is home to two distinct species of oak, Gambel oak (Quercus gambelii) and Dixie live oak (Quercus turbinella), which can hybridize to form an intermediate species that has been named Quercus undulata. In spite of previous research on these shrubs, we are still unsure of how the hybrid has survived in small isolated locations well outside of the range of one parent species (Dixie live oak, which is restricted in Utah to Washington, Kane, and San Juan Counties). Our primary objective was to perform an ecological niche modeling assessment of Q. undulata within the Three Peaks Recreation Area. Our approach included collecting plot-level data to determine abiotic and biotic niche factors correlating with the presence/absence of Q. undulata, including slope, aspect, soil type, soil chemistry, vegetation cover, and other associated woody plant species. Our results have shown that Q. undulata populations have higher concentrations in south-facing slopes, potentially reflecting the climatic preferences of its Dixie live oak parent. Results also showed that both focal species, Q. undulata and Q. gambelii, prefer areas with shallow soil and rocky outcrops. We plan to continue data collection and our analysis of soil type and chemistry. These results will help us to better understand the evolutionary history and ecological requirements of this fascinating native Utah plant.

Authors: Jane Damstedt, Kasey Cole. Mentors: Kasey Cole. Insitution: University of Utah. In this project, we are analyzing faunal remains recovered from Utah’s House Mountain Range in the Tubafore cave system to create a paleoecological baseline of life in that location over the past 1000 years. All animals have relatively consistent ecological tolerances and preferences, so comparing the data from the information obtained in this study with contemporary accounts of mammalian ecology in the same area will be useful in evaluating the role of anthropogenic climate change in mammalian ecosystems. In this project, I am identifying the animal remains to the lowest level of taxonomic classification possible to determine what kinds of species richness and environment were present at the time in which the animal was alive. Creating an accurate record of the animals found in this cave assemblage is critical because very little is known about the species richness of the Great Basin area. This data will then be stored and compared to modern zoological data, which came from online databases Actos and Vertnet. The information gathered from this project can be used to inform conservation and sustainability efforts aiming at preserving biodiversity in Central Utah’s Mountain Ranges.

Authors: Auriana Dunn. Mentors: Kathryn Sokolowski. Insitution: University of Utah. Reconstructing the diet of extinct animals can help us understand how living species are suited to their environments, which can inform paleoecological and zooarchaeological research as well as modern species conservation. Seasonal variation in animals’ diets can further be used to reconstruct seasonality patterns in the past and understand how animals may vary their diet under future climate change. However, determining the diet of extinct species is challenging, and detecting seasonal changes in diet is even more so. Dental microwear texture analysis (DMTA) can be a tool to solve this problem. DMTA analyzes dental microwear by measuring the pits and scratches on teeth to determine what an animal consumed in the days and weeks before it died. This project examines the DMTA of modern African Bovidae species to determine if seasonal dietary changes can be observed. If a difference can be found in the dental microwear of specimens that died during different seasons, seasonal changes in diet can be inferred. Here, I gathered dental microwear data from 407 specimens across five modern studies and matched the data to the museum specimens’ date of death to determine whether it died in a wet or dry season. The analysis found that four of the Bovidae species examined in our study showed evidence of seasonal diet shifts in their DMTA data. Dental microwear can be useful in determining seasonal diets and season of death for specimens. This method can be applied to extinct Bovidae species to understand ancient ecosystems and the evolution of Bovidae dietary strategies.

Authors: Adriana Bibo, Nicole Losurdo, Nichole Link. Mentors: Nichole Link, Cindy Greaves. Insitution: University of Utah. Microcephaly is a neurodevelopmental disorder characterized by occipital frontal circumference (OFC) more than two standard deviations below the mean. It can be accompanied by comorbidities including intellectual disabilities, seizures, and other developmental phenotypes. Microcephaly is attributed to malnutrition and exposure to toxins or infection during pregnancy, but genetic mutations are also a leading cause. To investigate the genetic mechanisms behind microcephaly, our lab collaborates with a physician who has completed whole exome sequencing for two hundred microcephaly patients. He identified potentially pathogenic human variants, which we are studying using Drosophila melanogaster as our model organism. We screened for loss of function phenotypes through RNAi knockdown of fly orthologs to determine if these genes are necessary for brain development. In our primary screen, we found that RNAi knockdown of Bx42 in neural stem cells or post-mitotic neurons causes significantly reduced brain lobe volume. In our current study, we are assessing potential causes for reduced brain lobe volume. We seek to determine if RNAi knockdown of Bx42 in neural stem cells or neurons affects cell number, proliferation, or death in developing brains.