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2024 Abstracts

Closely related Enterobacteriaceae species with antibiotic class and subclass resistance

Authors: Coleman Judd, Alexander Beagley
Mentors: Michelle Culumber
Insitution: Weber State University

Due to the overuse of antibiotics in industrial, agricultural, and medical settings, antibiotic-resistant bacteria are becoming more prevalent in various unique settings. We set out with the goal of identifying antibiotic-resistant bacteria from the Great Salt Lake waterfront. After isolating colonies on agar containing the antibiotic Tetracycline, we performed 16S rRNA gene sequencing and identified three separate Enterobacteriaceae species all exhibiting varying levels of antibiotic resistance. We performed an array of biochemical and physiological tests to further distinguish the Enterobacteriaceae species. Full spectrum biochemical tests indicate two near identical species followed by a third, novel species as both a phenotypic and biochemical outlier. Antibiotic resistant bacteria within the Great Salt Lake region present an ever-growing dilemma of clinical and environmental danger that accompanies antibiotic resistant microbes.