Authors: Parker Johns, Chandler Warr, Gregory P Nordin, William G Pitt
Mentors: William G Pitt
Insitution: Brigham Young University
Polyethylene glycol diacrylate (PEGDA) microfluidic devices have gained prominence in various biomedical and analytical applications due to their exceptional material properties and compatibility with cell culture systems. However, the presence of residual uncrosslinked PEGDA monomers and photoinitiators within these devices can lead to cytotoxicity concerns, potentially compromising cell viability and experimental results. In this study, we present an innovative approach to reduce cytotoxicity associated with PEGDA microfluidic devices by implementing an isopropanol (IPA) washing method.
Our investigation involves thoroughly characterizing the cytotoxicity of untreated PEGDA microfluidic devices and comparing it with devices subjected to the IPA washing procedure. We systematically assess cytotoxicity using cell viability assays and cell proliferation studies to quantify the impact of residual cytotoxic compounds on cells cultured within the microfluidic channels. Our results demonstrate that IPA washing significantly reduces the cytotoxic effects of PEGDA microfluidic devices, leading to improved cell viability and overall biocompatibility.
Furthermore, we elucidate the mechanisms behind the reduction in cytotoxicity, shedding light on the role of IPA in effectively removing unreacted PEGDA and photoinitiators. This study provides valuable insights into the optimization of PEGDA microfluidic device fabrication processes, enhancing their biocompatibility and usability for various biological and biomedical applications.
In summary, our research highlights the importance of addressing cytotoxicity concerns associated with PEGDA microfluidic devices and offers a practical solution through the implementation of an IPA washing method, ultimately expanding the potential of these devices in diverse scientific and clinical applications.