Authors: Tessa C Black, Craig D Thulin
Mentors: Craig Thulin
Insitution: Utah Valley University
Monarch butterflies (Danaus plexippus) are a distinctive and beloved species due to their unique wing coloration. In 2022, UVU student Kyri Forman and Dr. Craig Thulin identified seven pigments in monarch butterfly wings, three of which have not yet been identified in any other organism. The novel pigments are modified versions of xanthommatin, and their discovery implies the existence of presently unknown enzymes which catalyze the pigments’ biosynthesis. The western blot and its modified technique, the far western blot, are useful tools for identifying protein-protein interactions using antibodies and bait-proteins, respectively. To identify xanthommatin-binding proteins present in monarch butterfly pupae, we are developing a new blotting technique which we are calling the midwestern blot. This technique uses pigment molecules to identify the presence of pigment-binding proteins within a mixture. The midwestern blot technique will be validated is being validated using cytochrome C, hemoglobin, and myoglobin, three heme-binding proteins of known molecular weight and structure. Once validated, we will use the midwestern blot to identify xanthommatin-binding proteins extracted from monarch butterfly pupae. The midwestern blot will help promote future investigations into pigment-binding proteins, including the enzyme responsible for the novel pigments identified in monarch butterfly wings.