2020 Abstracts

Symons, J. David; Thompson, Lauren; Ramous, Caroline; Cho, JaeMin; Boudina, Sihem; Margetts, Alex; Buzianis, Skye; Park, Seul Ki; Luu, Kiana; Hansen, Michele; Pires, Karla; Whitehead, Kevin; Carter, Kandis; Buffolo, Marcio (University of Utah)
Faculty Advisor: Symones, J. David (University of Utah, Nutrition and Integrative Physiology)

Protein aggregates accumulate and organelles become damaged and / or dysfunctional during the process of healthy aging. A progressive loss of the cellular quality control mechanism autophagy (i.e., "self-eating") contributes to this age-associated decline in cellular function in many organs. Evidence for an age-associated repression in cardiac autophagy is not consistent. We hypothesized that 24-month old (old) male C57Bl6/J mice exhibit repressed autophagosome formation in the heart, an accumulation of cardiac protein aggregates, myocardial dysfunction, and reduced exercise capacity vs. 6-month old (adult) mice. First, cardiac lysates from old mice displayed reduced (p<0.05) accumulation of LC3II / GAPDH and degradation of p62 vs. adult animals (assessed via immunoblotting; n=12 per group). Second, the lysosomal acidification inhibitor chloroquine (CQ) induced accrual (p<0.05) of LC3II / GAPDH and p62 in hearts from adult but not old mice (quantified by immunoblotting; n=7 per group). Third, the number and size of protein aggregates was higher (p<0.05) in hearts from old vs. adult mice (measured via scanning electron microscopy; n=5 per group). Fourth, left ventricular mass / tibial length was greater (p<0.05), and indices of systolic, diastolic, and global left ventricular function (measured via transthoracic echocardiography) were impaired (p<0.05), in old vs. adult animals (n=12 per group). Finally, maximal workload performed during a treadmill-test, and soleus muscle oxidative enzyme capacity (citrate synthase activity assessed via ELISA), were less (p<0.05) in aged (n=11) vs. adult (n=12) mice. To determine whether late-in-life exercise training improves cardiac autophagy to an extent that demonstrates functional relevance, separate cohorts of older male mice completed a progressive-resistance treadmill-running program (old-ETR) or remained sedentary (old-SED) from 21-24 months. Body composition (estimated via nuclear magnetic resonance), exercise performance during a maximal workload test, soleus muscle citrate synthase activity, indices of cardiac antioxidant enzyme activity (quantified via immunoblotting), markers of cardiac autophagy, accumulation of cardiac protein aggregates, and indices of myocardial function, all improved (p<0.05) in old-ETR (n=11) vs. old-SED (n=12) mice. These data are the first to demonstrate that markers of cardiac autophagy are elevated, and indicators of protein aggregate removal and myocardial function are improved, in older mice that complete a treadmill-training regimen that is sufficient to increase skeletal muscle CS activity and maximal exercise capacity. Our results provide strong proof of concept to evaluate cause and effect relationships among exercise-training, myocardial autophagy, and cardiac function using genetic approaches in pre-clinical models and these studies are ongoing in our laboratory.

Hale, Kaysi; Anderson, Chris (Utah Valley University)
Faculty Advisor: Anderson, Chris (Utah Valley University, Psychology)

While there is significant data on adolescent sexual activity, education, and attitudes, there is little research done on adults who choose to stay abstinent, especially in the United States. Religiosity plays a significant role in reducing sexual behaviors before marriage. Many Christian faiths teach that sex should only be engaged in after marriage, so many followers choose to stay abstinent until marriage. Education about sex contributes to individuals choosing to stay abstinent and/or choosing to use safe-sex practices. Gender is not a large factor when considering reasons why individuals choose to stay abstinent; women have slightly lower rates of pre-marital sex at 94%; men at 96%. Sexual health and sexual well-being are difficult to define, but especially challenging when trying to define it for those choosing to stay abstinent into adulthood. The proposed study will sample an anticipated 200 self-reported abstinent undergraduates at Utah Valley University. This university has a high proportion of Christian students who are predominantly members of the Church of Jesus Christ of Latter-Day Saints. Data will be collected about why the students report they are remaining sexually abstinent as well as their sexual attitudes, beliefs, behaviors, and feelings about their own sexuality. Correlations can be drawn between religiosity, gender, age, perceived healthy attitudes towards individual sexuality, feelings about sexual stimulating behavior, among other factors. This paper will contribute to the standing data about religiosity and sexuality. It will enhance the literature about how abstinent adults feel about their own sexuality.

Gardner, Emily; Floyd, Eden; Taylor, Shaylee (Utah Valley University)
Faculty Advisor: Jensen, Francine (Utah Valley University, Nursing)

Problem Statement: Nurses experience a high incidence of workplace violence.

Purpose: The purpose of this qualitative research study aims to examine nurses' perceptions of workplace violence, including nurse-to-nurse, patient-to-nurse, and coworker-to-nurse violence, and explore nurses' perceptions of safety measures in their work environment.

Research Question: What are nurses' perceptions and experiences of workplace violence and what factors make them feel safer?

Background: Safety in healthcare is a concern that needs active improvement; "67% of all nonfatal workplace violence injuries occur in healthcare, but healthcare represents only 11.5% of the U.S. workforce" (Locke, Bromley, & Derspiel, 2018). The Joint Commission (2018), an accrediting body for many healthcare organizations, reported that nurses are among the most victimized by violence in healthcare. Certain locations such as the emergency department, psychiatric units, geriatric units, and intensive care units may experience a greater number of violent incidents (Camerino, Estryn-Behar, Conway, van der Heijden, & Hasselhorn, 2008). One study showed that 64% of the nurse respondents felt like violence, both physical or verbal, was an expected part of their jobs (Copeland & Henry, 2017). In addition, it was demonstrated that many nurses accept a level of violence on the job and do not see the importance of reporting incidents and looking for solutions (Copeland & Henry, 2017).

Hope to accomplish: The course of this research will explore how nurses perceive their workplace safety, shed light on experiences of workplace violence they have witnessed, and their perceptions of on-the-job safety. This research will also examine what makes newer and older nurses feel a greater sense of security while performing their jobs. Since some of the factors that make nurses feel safer may involve unit or hospital policies, these findings may offer ideas for potential changes to policies to help improve workplace safety in healthcare.

DeNiro, Adara; Cao, Erhu; Wang, Qinzhe; Wang, Jinliang (University of Utah)
Faculty Advisor: Cao, Erhu (University of Utah, School of Medicine)

The kidneys are responsible for several essential bodily functions critical to our survival. These include filtering blood and excreting metabolic waste into urine. Two proteins, polycystin-1 and polycystin-2, interact in renal tubules and promote the normal development and function of the kidneys. However, the proteins' actions are not well understood. Polycystin-1 and Polycystin-2 are encoded by the PKD1 gene and the PKD2 gene respectively. A mutation in either of these genes can lead to Autosomal Dominant Polycystic Kidney Disease (ADPKD), a common inherited disease leading to progressive renal failure. Several extrarenal manifestations occur as a result of ADPKD including hepatic cysts and intracranial aneurysms. Ultimately, ADPKD often leads to end-stage renal disease that requires dialysis or transplantation. Currently, there is no cure for ADPKD due to setbacks regarding structural determination of the Polycystic Kidney Disease Proteins. Some setbacks are the inability to view the regulatory cytosolic domains of the PKD1/PKD2 channel in atomic detail and understand the function(s) of the polycystic kidney disease proteins. My project examines the ability of Fabs and nanobodies to bind to the PKD2 channel and the PKD1/2 complex as this potentially facilitates structural determination. This is done by stabilizing cytosolic domains and/or promoting a more uniform orientation distribution of these proteins essential for imaging by cryo-electron microscopy.

Chandler L Eyre. David A. Eastley (Brigham Young University)
Faculty Advisor: Hancock, Chad (Brigham Young University, NDFS)

Purpose: To determine the effects of H2O2 exposure to human hepatocytes on the iron regulatory proteins such as transferrin receptor (TfR), Ferritin Light-Chain (FLC). Methods: Cells were cultured at an initial density of 35,000 cells/cm2 in 6-well plates, grown until confluent, and then harvested. Iron treatments were done using FeCl3 solution with media to desired concentrations, and then incubating for 24 hours. Groups that received both iron and H2O2 treatments were exposed to 12 hours of iron, followed by incubation with media that included both iron and GOX at 4.8 mU/mL and 0.165 mg/mL of CAT (2-5 KU/mg). BCA protein assay was used to assess protein content, and then normalize each sample to each other for subsequent preparation for western blot. Western blots were carried out were analyzed by fluorescence following densitometry. MTT assays were carried out utilizing the mitochondrial reductase enzyme, and colorimetrically measured to assess cell viability. Results: Iron treatments of 10, 50, and 100 µM for 24-hrs did not result in any significant cell death. Treatment with the same concentrations resulted in a significant decrease (n=12) in TfR for all three groups when compared against control cells that were cultured and harvested simultaneously without any addition the growth media. (10 µM p<0.01), (50 µM p<0.01), and (100 µM p<0.01). FTL in the 10 µM group was significantly decreased (n=11, p<0.01), but not for the 50 µM, or 100 µM groups with p values of 0.9867, and 0.9612, respectively. H2O2 treatments produced concentrations of 5-10 µM, mimicking neutrophil release during inflammatory response. Conclusion: Our assay is sufficient to mimic neutrophil release of H2O2. Iron treatments are able to induce TfR decrease but seem to have a threshold at 10 µM for increasing FTL for storage. We hypothesize that this may be due to a dysregulation at higher concentrations.

Seaboch, Melissa (University of Utah)
Faculty Advisor: Seaboch, Melissa (University of Utah, Anthropology)

Primates do not make good pets because they are highly social animals that require members of their own species, large amounts of space, and specific diets to maintain positive psychological well-being. Additionally primates can also be aggressive toward their owners and they can transmit diseases (e.g. Salmonella, parasites, etc.) to their owners. Even so, there are over 15,000 pet primates in the United States. Regulations for owning a primate vary from state to state from a complete ban (19 states) to no restrictions (17 states). The goal of this project is to understand the pet primate trade and ownership in the United States, specifically, in which states they are for sale. Data (type of primate for sale and location) were collected bimonthly for five months from six online exotic pet trade websites. We found that 311 primates were offered for sale in 11 states with Florida having the most primates for sale (62%, n=195) followed by Tennessee (8.6%, n=27), and Texas (8%, n=25). The number of primates for sale in Florida is surprising since Florida requires permits from the Florida Fish and Wildlife Conservation Commission while there are no regulations for owning monkeys in Texas, or Tennessee. The Captive Primate Safety Act currently before Congress would prohibit the sale of all nonhuman primates by including them as "prohibited wildlife species" and would greatly reduce the ownership of primates as pets, though we note that a similar bill failed in 2007.

Jensen, Afton; Claunch, Kelsie; Verdeja, Marco; Dungan, Matthew; Anderson, Shellie; Clayton, Colter K.; Goates, Michael, Thacker, Evan (Brigham Young University)
Faculty Advisor: Thacker, Evan (Life Sciences, Public Health)

The purpose of this paper is to provide a systematic review of lay perspectives on the meaning of successful aging from older adults across multiple cultures and countries. Furthermore, this article allows for a discussion of the complexity of older adults' perspectives on successful aging and how successful aging may be defined differently across cultures and countries. We conducted a systematic literature review to identify, summarize, and evaluate peer-reviewed studies of qualitative data on lay perspectives of older adults. This review included studies on elderly populations published from 2010 to 2017 that addressed older adults' lay perspectives on successful aging. Such studies involved primary research performed in the United States, Germany, Singapore, Lebanon, Mexico, Canada, and New Zealand. The key concepts that emerged from older adult responses across all studies included physical health, cognitive health, social engagement, attitude/coping, independence/security, and spirituality. The overall emerging themes from our data suggest that older adults were not as concerned with physical health as biomedical previous research of successful aging often suggest. Rather, older adults focused on maintaining positive attitudes in order to cope with life changes, valuing social engagement and contribution as more essential to successful aging. Respondents from Western Europe valued keeping positive attitudes about death and focusing on gratitude instead of worries. Additionally, respondents from the United States and Mexico placed importance on pursuing activities and interests and having strong involvement with family and friends respectively. Noting differences, respondents from the Middle East, Asia, and Oceania Region, as opposed to respondents from North America, moreso prioritized the absence of chronic disease and comfort in one's own environment over keeping active. By providing a more comprehensive organizational framework of older adults' qualitative responses to successful aging, we better understand what successful aging means across cultures.

Holden, Maliea; Richardson, Spencer; Chilom, Gabriela (Dixie State University)
Faculty Advisor: Chilom, Gabriela (Dixie State University, Physical Sciences)

Humic acids are a key component of the terrestrial ecosystem as they are responsible for many physical and chemical properties of soils; buffering capacity, metal-binding capacity, transport and fate of contaminants, stability of aggregates of soil particles and water-holding capacity all depend on the amount and nature of humic substances in a soil.

The current project is looking into the properties and ability of humic acids extracted from leaves, to improve the stability and fertility of a mineral soil. The humic acid was extracted from fresh and aerobically incubated leaves by traditional alkali extraction. Three species of trees commonly grown in St George, Utah were selected: Prunus x Cistena, Pyrus Calleryana Bradford, and Chilopsis Linearis. The extracts were characterized by the amount and nature of their carbon content as determined by IR, 1H NMR, and 13C NMR spectroscopy. Their properties showed similarities and differences with humic acid extracted from mineral soil and leonardite.

Parsons, Travis; Lippert, Peter; Bartley, John (University of Utah)
Faculty Advisor: Lippert, Peter (University of Utah - College of Mines and Earth Science, Geology & Geophysics); Bartley, John (University of Utah - College of Mines and Earth Science, Geology & Geophysics)

Field observations and geochronological measurements of plutons in Yosemite Valley suggest that plutons grow incrementally as a series of stacked sheets of smaller intrusions (i.e., dikes and sills) (Coleman et al., 2004; Glazner et al., 2004; Bartley et al., 2006). This interpretation is in contrast to the traditional view of pluton emplacement through crystallization of a single, massive magma chamber. Most of the observations supporting incremental pluton emplacement use the relationship between zircon U-Pb dating of pluton sections and estimated granitic magma cooling rates to argue that a single magmatic event would crystallize significantly faster than the geochronologic data permit. Incremental pluton emplacement also predicts specific relationships between the age of intruded sheets of magma and the original orientation of these sheets, such that older sheets are expected to be tilted or deformed more than younger sheets. Here we test this prediction of differential tilting by measuring the paleomagnetic inclination preserved in well-dated and structurally characterized sheets of the Tuolumne Intrusive Suite. Magnetic inclination provides a tilt-meter with respect to the Earth's magnetic field direction at the time of pluton emplacement; the reference inclination assuming an untitled pluton is known from independent data sets. We also present rock magnetic data (temperature-dependent magnetic susceptibility, magnetic remanence characteristics) and results from petrographic investigations to characterize the mineralogy and stability of the magnetization. Our results suggest that the low-titanium magnetite remanence carriers are primary and are not biased by secondary magnetizations. The distribution of magnetic inclinations in our sample set — in which older sheets on the periphery of the pluton are shallower than those in younger, more interior sheets, and with respect to the reference inclination — is consistent with predictions from the incremental pluton emplacement hypothesis.

Dearden, Heravi, Shen, Arslanian, Shrestha, Mismash, Tinsley, Pay (Brigham Young University)
Faculty Advisor: Dearden, David (Brigham Young University, Chemistry)

Using the Spartan and Ion Molecular Spectrometry Suite (IMoS), we use the 3D modeling to predict stability and molecule favorability. Previously in our experimentation we have seen and proper capping of a host molecule with cucurbit[5]uril. This was different than cucurbit[6]uril as the cap in some cases didn't bond to all the upward oxygens because on the molecular symmetry, thus host enclosure was less favorable in cucurbit[6]uril than cucurbit[5]uril. We also observed in our lab the energy differences using the Extensible Computation Chemistry Environment (ECCE) of cucurbit[5]uril host transfer based on cap and host interactions. These studies show that cucurbit[5]uril with methane as a host is more favorable than outside the cavity. It also shows that methane inside cucurbit[5]uril is more favored than in decamethylcucurbit[5]uril. The studies using computational study will be testable using mass spectrometry, and we predict that the same behavior trend will show using other caps and hosts with cucurbit[5]uril and decamethylcucurbit[5]uril.

Cromar, Zachary J.; Findlay, Matthew; Turner, Elizabeth; Mills, Ammon (Utah Valley University)
Faculty Advisor: Gazdik-Stofer, Michaela (College of Science - Utah Valley University, Biology); Sylvester, Steven (College of Humanities and Social Sciences - Utah Valley University, Political Science)

The World Health Organization has included vaccine hesitancy in the top ten threats to global health in 2019. Studies done in the United States have shown that the Moral Foundations Theory (MFT) can be used to identify why individuals are more vaccine-hesitant (VH) than others. Counterintuitively, the dichotomous moral foundations (MF) of care vs. harm, traditionally used in pro-vaccine messaging interventions, or fairness vs. cheating, were not found to be the foundations on which VH parents based their decision not to follow the CDC recommended vaccination schedule. It was discovered that the purity vs. degradation and liberty vs. oppression foundations are more important to VH individuals than all other foundations. Highly VH individuals are twice as likely to emphasize purity and liberty. Concerningly, in 2018, Utah ranked in the bottom ten states for child vaccination rates in 11 of the 19 vaccines reported by the Utah Department of Health. We plan on testing a broader messaging intervention than the current, traditional vaccine messages. Our messaging interventions will emphasize the MFs of liberty vs. oppression and purity vs. degradation to see if they will be more effective than our more traditional messaging intervention emphasizing care vs. harm, or an unrelated control message not related to vaccines. We hypothesize that messages emphasizing the purity and liberty foundations will resonate better with the VH and decrease their vaccine-hesitancy relative to the other groups. If our data supports that the MFs of liberty vs. oppression and purity vs. degradation significantly decrease vaccine-hesitancy than current general vaccine education messaging interventions should be broadened to include these MFs. However, if results do not demonstrate that the liberty vs. oppression MFs are more effective at decreasing vaccine hesitancy, then more research should be performed on the subject.

Tilisa Howell; David Aguilar-Alvarez (Weber State University)
Faculty Advisor: Aguilar-Alvarez, David (Moyes College of Education, Athletic Training & nutrition)

Abstract:

Background:

Recent studies show that water intake plays a major role in the development of chronic diseases such as type 2 diabetes, cardiovascular disease, and kidney disease. The aim of this study was to determine if the percentage of total fluid intake from water influences Metabolic Syndrome (MetS) and identify significant associations between water, dairy, soft drinks and juice consumption with MetS parameters in college students.

Methods:

We measured MetS parameters and collected diet records from 364 college students, ages 18-65 years. Participants were grouped by gender (Male: 112; Female: 252) and by percentage of total fluid consumption from water. Groups were defined as high (>70%) percentage of total fluid intake from water (HPW) and Low percentage (<30%) of total fluid intake from water (LPW). T-test was used to determine mean differences in MetS parameters between HPW and LPW, and Pearson correlations to determine associations between MetS and specific fluids.

Results:

HPW participants showed lower diastolic blood pressure when compared with their LPW counterparts (µ=78. 51±8. 28 vs µ=81. 2± 9. 28, p=0. 05). Men dairy fluid consumption was associated with increased fasting blood glucose (r=0. 242, p=0. 01. In women, Juice consumption was associated with increased LDL-Cholesterol (r= 0. 205, p=0. 02).

Conclusion:

Consuming non-water fluids showed negative effects on blood pressure. Contrary to previous studies, dairy fluid consumption in men was associated with increased blood glucose. It is possible that high-glycemic foods tied to dairy consumption modulate this association. Our results in women are consistent with previous studies were juice consumption increases triglyceride production and VLDL-Cholesterol.

Keyes, E. Dalles; Alvey, Brighton; Smith, T. Andrew; Roberts, Andrew G. (University of Utah)
Faculty Advisor: Roberts, Andrew G. (University of Utah, Chemistry)

Medicinal chemistry has long relied on the development of small molecule therapeutics to treat human disease. Small molecules affect change at the cellular level through specific interactions with biological targets (e.g. proteins), thereby eliciting a desired physiological response. Conversely, small molecules can also interact non-specifically, which can complicate their targeted application. In many cases, the use of peptide-based medicines can address this limitation. Like small molecules, peptide-therapeutics are designed to modulate specific biological processes. They often exhibit desirable activity at low concentrations as a result of high selectivity. Being comprised of natural amino acid building blocks, peptides offer an inherent advantage. Their natural breakdown leads to minimally toxic degradation products. However, premature and rapid degradation can result in failure to reach an established target in vivo. The cyclization of peptides has shown to be a promising strategy to address this problem. Inspired by Nature's wide collection of non-ribosomal peptides, specifically those comprising electron-rich aromatic moieties, we have developed a new chemical strategy for the synthesis of cyclic peptides. Our cyclization method leverages the inherent reactivity of the tyrosine (Tyr) phenol nucleus with electrophilic 1,2,4-triazoline-3,5-dione (TAD) moieties. Using this reaction, we can construct macrocyclic peptidomimetic scaffolds. Upon synthesizing an N4-substituted 1,2,4-triazolidine-3,5-dione (aka urazole) at the N-terminus of a solid-supported peptide, the urazole moiety is chemoselectively oxidized under mild conditions to generate a TAD derivative in situ. The TAD moiety reacts with the sidechain phenol nucleus of internally or terminally located Tyr residues and results in the formation of a macrocyclic peptide. We envision that this method will significantly augment current strategies for constructing macrocyclic peptides by enabling the facile synthesis of complex peptidomimetic scaffolds. Furthermore, this approach is anticipated to expand the repertoire of tools used for developing medicinally relevant peptides and, thus, may be suitable for preparing unique peptide-based therapeutics.

Sevy, Jonathon; Radebaugh, Jani; McDougall, Dylan; Kerber, Laura; Rabinovitch, Jason (Brigham Young University)
Faculty Advisor: Radebaugh, Jani (Brigham Young University, Geology)

Yardangs are wind-carved linear ridges that are found selectively on Earth and extensively on Mars. The history of the morphologic development of yardangs is not well known. In the Puna high plateau of Argentina there are ignimbrite deposits, many of which have been eroded into yardang fields, commonly called fleets. Yarding fleets are evenly spaced forms, facing into the wind, that resemble a fleet of boats sailing. A prominent fleet, known as Campo de las Piedras Pomez (CCP) was studied to more fully understand their formation and morphology, including field research in December 2018 and 2019. Some physical characteristics looked at in the field included: dedos direction and lengths; heights and lengths of yardangs; structural features; wind and gravel ripple direction. In addition, relationships between yardang organization and structural control have been analyzed. The dedos on the fronts of yardangs averaged 4.0cm in length, while the reverse dedos were 3.3cm. From this it was determined that: Two wind directions, with one dominant, are present; Structural features can shape morphology of yardangs.

Parada, Michelle (Weber State University)
Faculty Advisor: Schramm, Katharina (Weber State University, Botany)

This research focuses on anti-tumor and the biotoxicity of A. millefollium (yarrow).
Yarrow is an interesting plant with a long history of medicinal uses, but very little has been done to research and prove the different beneficial properties claimed. There are two simple bench-top assays performed in this study, the potato disc assay which inoculates potato slices with Agrobacterium tumefaciens as well as different concentrations of yarrow extracts and assesses the resulting tumor formation and the brine shrimp toxicity assay to assess the LD-50 of the extracts. My results will indicate whether or not the native yarrow extracts inhibit or do not inhibit tumor formation and its toxicity level. The results of this research could lead to further studies of the phytochemicals in the plant to be used for cancer treatments.

Asay, Spencer; Graham, Andrew; Burke, Lexady; Barnes, Brad; Oblad, Richard; Kenealey, Jason (Brigham Young University)
Faculty Advisor: Kenealey, Jason (Life Sciences; Nutrition, Dietetics, and Food Science)

Prostate cancer is the second most commonly diagnosed cancer in men, and metastatic prostate cancer is currently incurable. Prostate cancer frequently becomes resistant to standard of care treatments. Moreover, the administration of conventional chemotherapeutic drugs, such as docetaxel (DOC), poses the risk of debilitating toxic side effects. Combination therapy, in which several compounds targeting multiple cellular pathways are administered jointly, is one tool that can be used to combat therapeutic resistance and drug toxicity. Vitamin E (VE) compounds and analogs have been proposed as potential non-toxic chemotherapeutics. We modeled combination therapy using mixture design response surface methodology (MDRSM), a statistical technique designed to optimize mixture compositions, to determine whether combinations of three chemotherapeutic agents (γ-tocotrienol (γ-T3), γ-tocopherol ether acetate (ɑ-TEA), and DOC) would prove more effective than DOC alone in the treatment of PC-3 human prostate cancer cells. A response surface was generated for cell viability, and the optimal treatment combination for reducing cell viability was calculated. We found that a combination of 30 µM ɑ-TEA, 20 µM γ-T3, and 25 nm DOC was most effective in the treatment of PC-3 cells. We also found that combining γ-T3 and ɑ-TEA with DOC decreased the dose of DOC required to significantly reduce cell viability in PC-3 cells. Finally, we found that combining γ-T3 and ɑ-TEA with DOC enhanced treatment efficacy in DOC-resistant PC-3 cells.

Ouzts, Ethan (Brigham Young University)
Faculty Advisor: Matheson, Rebekka (BYU Family, Home, and Social Sciences; Psychology)

Ketamine was recently approved by the U.S. Food and Drug Administration as a therapeutic approach to treat individuals with treatment-resistant depression. This approval opens the door for other hallucinogens to be approved for psychiatric use. This review compares the antidepressant efficacy and safety of ketamine to serotonergic hallucinogens, such as lysergic diethylamide acid (LSD). Ketamine acts as the standard of comparison in this review. Serotonergic hallucinogens demonstrate similar short to mid-term responses in patients with depression and compares well to ketamine's safety. Researchers should conduct additional randomized, controlled experiments to better establish serotonergic hallucinogens' antidepressant potential. Despite this limitation, serotonergic hallucinogens warrant serious consideration for potential antidepressant treatment.

Sundstrom, Rebecca; Jensen, Joseph B. (Utah Valley University)
Faculty Advisor: Jensen, Joseph (Utah Valley University, Physics)

We will measure Surface Brightness Fluctuations (SBF) in three galaxies (ESO137-G006, NGC 3309, and NGC 5128) using images from two cameras: the GeMS Multi-Conjugate Adaptive Optics (MCAO) system and the FLAMINGOS-2 (F-2) near-infrared imaging spectrograph, at the 8-meter Gemini South Telescope in Chile. To make an accurate measurement we need to first determine a photometric calibration for the MCAO system by comparing flux, or captured light, from both detectors. This is necessary because MCAO collects crisp, sharp images but omits some of the total incoming flux from the stars in the galaxies. F-2 takes broader images but includes more measurable flux. By finding the ratio of the flux collected by MCAO to the flux collected by F-2 we can take this "missing" light into account without compromising the crisp, sharp resolution MCAO provides.