Nelson, Andrew; Foutz, Isaac; Hunt, Porter; Wood, David; Bundy, Bradley; (Brigham Young University)
Faculty Advisor: Bundy, Bradley (Brigham Young University, Chemical Engineering)
Cell-free Protein Synthesis (CFPS), an in vitro system for producing recombinant protein, is a rapidly expanding field. To date, applications of this technology, among others, include unnatural amino acid incorporation, protein microarray fabrication, genome engineering, and the production of therapeutics, vaccines, and biocatalysts. Here, we further engineer cell-free protein synthesis as a biosensor for endocrine-disrupting chemicals (EDCs), compounds that mimic hormones and thus disrupt endocrine system physiology in the body.