2015 Abstracts

Emily Kunz, Shea Long, Melany Reeder, and Brigid Crotty, Utah State University Education It has been proposed that asking a child to make up their own story, rather than to retell a story, is a more stringent test of narrative ability and may tax the linguistic system revealing weaknesses not apparent in less difficult contexts (eg., retelling stories). At least one study has shown that children with Autism Spectrum Disorders (ASD) experience content-form tradeoffs as they master narrative discourse (DeLucchi, Fricke, Kaye, Crotty and Gillam, 2015). The content-form tradeoff was observed when children with ASD with typical grammatical skills and poor narrative proficiency were shown to experience significant grammatical difficulties as they mastered narrative discourse. The purpose of this study was to determine whether content-form trade-offs were observed in stories children with ASD were asked to retell. Five children with ASD ranging in age from 9-12 were asked to retell stories weekly, during a baseline and narrative treatment period over the course of 12-16 weeks. The stories were scored for grammaticality and narrative proficiency. Story retells were observed to be grammatical whether elicited during baseline, early, mid or later treatment sessions. Children with lower language skills experienced times when they were completely unable to recall a story, particularly early on in instruction, although when they did, they experienced good grammatical accuracy. Children with higher language skills were always able to remember parts of the story and were highly grammatical. The story model (retell) may make it less difficult for students with ASD to focus on and remember content while also maintaining grammatical accuracy.

Mary Ann Hammon, Sydney Sneddon, Madeline Williams and Brigid Crotty, Utah State University Education Children diagnosed with ASD often experience marked difficulty in the comprehension and production of narrative discourse that extends well into their adolescent and adult years (7, 8, 9, 10). These narrative difficulties appear to be linked directly to the core symptoms of ASD that manifest in failure to plan using information from multiple sources, a hyper-focus on details at the expense of gist-level propositions and limited use of mental state and causal language to encode goals and motivations of characters (11). Theory of Mind (ToM) accounts propose that a core deficit in ASD is an inability to infer the emotional or mental states of others. Deficits in ToM have been shown to significantly impair one’s ability to engage in ongoing social interactions and to develop the linguistic knowledge (e.g., mental state and causal language) necessary for understanding the relationship between events in discourse (9). Mental state and causal language is necessary for the establishment of a causal framework to link story grammar elements together. The overarching goal of this project was to test whether a program designed to teach narrative language skills was effective for increasing the use of mental state and causal language for children with high functioning autism (ASD). A multiple baseline across participants study was conducted with 5 children with ASD (ages 8-12). Intervention was provided for two 50-minute individual sessions per week for a total of 21-33 sessions (depending on the student). Children’s spontaneous stories and story retells, collected weekly, were analyzed for the use of mental state and causal language before, during and after intervention. All of the children made clinically significant gains after participating in the instruction, with clear changes in the use and complexity of mental state verbs during both types of narrative production tasks (story retell, spontaneous generation). The gains were maintained after intervention was discontinued.

Kassandra Sqrow, Weber State University Education Opportunities for children to interact and connect to the natural environment through play are declining. The benefits of outdoor play are well documented (Little and Wyver, 2008) and show the important role it contributes to healthy child development. Yet, fears and anxieties parents have about the outdoor environment are the most potent forces that prevent parents from allowing their children to play outdoors (Furedi, 2002; Louv, 2006). Identifying the beliefs and attitudes parents have about outdoor-based play can provide valuable insights for recreation and youth professionals to understand how to encourage outdoor play in families. Therefore, the purpose of this study was to explore parents’ perceptions on factors that constrain or facilitate the level of outdoor play that they encourage in their children. Parents with children ages 4 to 10 years old were recruited through a local school to participate in focus groups. Research participants were asked about their perceptive on the role outdoor-play has on their child’s development and characteristics of outdoor play spaces that influence the level of outdoor play their children engage in. Data will be analyzed by identifying recurring themes and patterns of parents’ perceptions and factors that influence the level of outdoor-based play in their child’s life. The data for this study is currently being collected and will be analyzed by November 2014. Implications for practice will be discussed.

Claudy Eckardt, Weber State University Education Obesity is a growing epidemic in the United States. Statistical facts show that one third of U.S. children, between the ages of 2-19, are obese. A common health risk found in children who are overweight is that they will continue to stay overweight throughout the course of their lifespan. Long-term health consequences of obesity only to be found in adults have become more prevalent in children as well. Prevention is the key to reducing this dangerous epidemic and its consequences. The purpose of this study was to investigate children’s knowledge on the causes and consequences of being overweight. This study used a qualitative research method. Two participants were interviewed for case studies. Both participants were between the ages of 8 and 11 and were from different ethnic backgrounds. Each child was given nine questions to answer. Each child was given an adequate amount of time to thoroughly understand each question and respond. Interviews lasted between five to ten minutes. Parental consent was given before the interview process. Results showed that the participants were exposed to the risk factors of obesity. Each child demonstrated a clear understanding that obesity has negative health consequences and expressed preventative measures as well. Furthermore the participants proved that obesity was not only limited to the school or home environment but multiple environments.

Are classic, young-adult novels set in the American west a dead genre in the American classroom? Despite its historical impact on popular culture, young-adult novels set in the American west have seen a considerable decline in relevance in American public schools. While classic young-adult literature receiving heavy rotation in current young-adult classrooms, as well as young-adult literature published in the current century, may have its place, Western, young-adult literature published in the 1960’s and 1970’s should still be read, taught, critiqued and celebrated. The general argument against including western, young-adult literature on the reading lists of public schools in the western states, where, arguably they should be the most prevalent, is that the texts are significantly passé and therefore not relevant to contemporary secondary students. While the majority of criticism in favor of western literature is also dated, this paper makes the argument that although the texts and various criticisms may seem behind the times, it should also be taken into account that present-day society is remarkably similar to society when the texts were initially published. Interestingly, not only is present-day society similar to society in the late 1960’s and 1970’s, but a recent interest in regionalism as opposed to vast commercialism also seems to be making an impact on society. By presenting literature that draws attention to the history of a local area, a literary gap created by an interest in who we were as a society and where we came from may be filled. The research conducted takes the classic, western, young-adult novel, True Grit by Charles Portis and that text with a more popular and more contemporary novel, The Outsiders by S.E. Hinton. It’s through this comparison we are able to appreciate and understand the relevance of classic Western literature in the contemporary classroom.

Samantha DeLucchi, Telesha Fricke, Kamilla Okey and Brigid Crotty, Utah State University Education Children with ASD often experience marked difficulty achieving proficiency in narration, and often require explicit instruction to learn this important discourse skill. The present study was designed to extend the work of Colozzo et al., 2011 by examining the relationship between content and form in the narratives of school-age children with ASD as they participated in a narrative intervention program to improve their knowledge of story structure and ultimately, to improve their ability to create coherent, organized narratives. Children received two, individual, 50-minute intervention sessions weekly for a period of about 7 – 11 weeks. Children were asked to make up their own stories once weekly. These stories were scored for narrative proficiency and for grammatical accuracy. Findings revealed that prior to beginning narrative treatment, all of the children’s grammatical accuracy was high while their narrative proficiency scores were low. In the first weeks of treatment, all children experienced a significant decrease in grammatical accuracy (<70%), however their narrative scores were observed to increase. Narrative proficiency scores continued to increase and become stable for all children. Interestingly, grammatical accuracy returned to normal (90% or greater) during the last weeks of intervention as children’s narrative proficiency became stable. The findings from this study support the presence of a content-form tradeoff, as children learn difficult linguistic skills, other skills that are ordinarily stable, may fluctuate until the new skill is mastered. The absence of grammatical errors may not be taken as an indication that the student is proficient in constructing a coherent, organized narrative. Further implications are discussed.

Taylor Anderson, Megan Israel Sen, Amy Nielsen, and Brigid Crotty, Utah State University Education Children with Autism Spectrum Disorders (ASD) have been shown to use substantially more simple sentences as compared to complex sentences in their spontaneously generated narratives (Sanford, Pearson, Summers, Crotty and Gillam, 2015). However, Sanford et al., showed that children with ASD began to use substantially more complex sentences in their stories during and after narrative treatment (>50%). It is possible that children with ASD may experience greater difficulty using complex sentences in stories they must generate than in retelling stories they have heard. The purpose of this study was to examine story retells of 5 children with ASD before, during and after narrative intervention for syntactic complexity. Results indicated that during baseline when children were not receiving instruction, their story retells contained more simple sentences than complex sentences. The use of complex sentences was observed to increase as children became more proficient in their narrative production skills. When compared to stories children generated on their own (spontaneous stories), the story retells contained more complex sentences overall, but were often associated with less verbal fluency particularly as children were mastering narrative skills. The findings will be discussed in terms of trade-offs in verbal fluency, grammaticality and the use of complex sentences during different stages of narrative proficiency as a function of initial language knowledge.

Duncan Stewart, University of Utah Communications Space is used as a rhetorical mechanism in Salt Lake City to separate the lives of the wealthy and the precarious bodies that are marginalized as hungry, unemployed, and homeless. This separation sustains a self-reproductive system of exclusion fueled by an unquenchable desire for profit and spatial separation. One way this separation is articulated is around the notion of “home,” insofar as the housed and the homeless represent this separation and are sustained by the political economy of the city. While the state efforts to address homelessness are valuable, the scope of the homeless problem requires that we critically reflect on how anti-homeless programs demand we conceptualize homelessness and the place of people experiencing homelessness in the space of the city. I will argue the space of the city is organized as a sorting mechanism that reinforces class and material divisions. Spatial separation becomes a regulatory operation where those who appear potentially able to participate in the economics of the cityscape are welcome and those who are not become legally excluded. One way this is accomplished is by enacting policies that promise to “solve” the problem of homelessness. Thus I will use Salt Lake City’s housing first initiative as lens to address the material consequences of such rhetorical force. Following this, I will highlight some of the major rhetorical themes that emerged in the analysis of discourses surrounding “Housing First.” Finally, I will consider how these insights help further an understanding of homelessness, expose how contemporary responses reify the marginalization of homeless populations from urban life, and point toward new ways of conceptualizing solutions to the “homeless problem.”

Samantha Tommer, Dixie State University Communications This study examines how heavy media coverage of court cases may produce a social stigma towards defendants that are found innocent in the criminal justice system. Trial by media is a central focus in this study on how court cases portrayed to media audiences influence information gathering and analyzing abilities. Since audiences only see certain frames of media, media court coverage may cause audiences to develop a negative stigma towards acquitted individuals by not seeing all aspects of the case, listening to analysts’ opinions, and receiving bias information through various news agencies. The study evaluated the responses of two groups of participants. The control group viewed a neutral video clip while the experimental group viewed a video of media court trial coverage and reporter analysis. Participants then answered a question regarding their level of comfort regarding if an individual accused of murder moves into their neighborhood. The researchers hypothesized that those participants who watched the media clip would rate their level of comfort much lower than those who watched the neutral clip, thus producing a social stigma towards the acquitted individual. After three weeks of watching the clip, all participants were contacted to and asked the same question to measure if the stigma had lessened and if so, how much.

Erin Olschewski, University of Utah Communications In the realm of entertainment education and media studies, there is a sizable amount of research linking children’s nutrition and early educational television shows; Sesame Street being one of the most commonly studied television programs. However, there is no work that attempts to connect nutrition with sustainability and social justice in the context of children’s educational television, despite the fact that the portrayal of these issues in the media is incredibly significant, especially in regards to children and their understanding of these complex topics. In my research, I am thematically analyzing the ways in which Sesame Street relays important messages about nutrition, sustainability, and social justice to its viewers. After a primary viewing and coding of three seasons and online food-related content, I have concluded that while health is being tackled in many episodes, issues surrounding sustainability and social justice are being neglected. As my research continues I will be analyzing these gaps on a deeper level to examine motivations behind the lack of content in these two crucial issue areas. Furthermore, this study connects the often disjointed fields of food studies, media studies, and environmental and health communication and provides a more holistic perspective on how these important topics are being conveyed, or not, to our children.

Chet Norman, Dixie State University Communications Maintaining alumni relationships can be difficult with a changing institutional culture and identity. For example, the name change from Dixie State College to Dixie State University (DSU) and change of the mascot’s identity, from the Rebels to the Red Storm, has caused alumni to become detached from the institution they once knew. This study, conducted in coordination with DSU’s Alumni Office, investigates strategies to communicate and foster relationships with discouraged alumni. A dynamic outreach strategy, based on academic theory and research from the disciplines of human communication and business marketing was developed to reach this goal. In particular, uncertainty management theory (UMT) and narrative storytelling methods were employed to develop a marketing campaign to further involve disheartened alumni through YouTube videos, monthly e-newsletters, alumni card program, social media interaction, and contests. This presentation will consist of a brief overview of the history of change in DSU’s identity, application of theories used to decrease alumni uncertainty, and lastly an identification of strategies for implementation.

Skyler Hunt, Dixie State University Communications This critical film piece examines Walt Disney Animation Studio’s Frozen through a lens of identity construction. By examining the film’s context, music, and dialogue, the identity formation of principal characters Elsa and Anna are interpreted as each identifies with different audiences. At the core of Elsa’s identity is a struggle with suppression incited by social expectations, linking her character to marginalized groups, such as the trans* community. Anna’s identity is seen forming in isolation through images displaying gender expectations, resulting in her cisgender status and role as an ally to her disenfranchised sibling. The interplay of these identities is also interpreted as a social appeal to audiences for acceptance of diverse internalizations of identity.

Michael Nagy, Dixie State University Communications The sketch TV show Saturday Night Live has, since its inception, produced actors and actresses that have gone on to create and act in comedic films. Mike Myers wrote and acted in the second SNL sketch to become a film, Wayne’s World (1992). The first was The Blues Brothers (1980). Through writing the central character of Wayne Campbell, Mike Myers explored the idea of the unattractive hero. Most of Myers’ post-SNL characters are in opposition to the stereotypical idea of a hero. This stereotype is a strong, tall, bold, outgoing, courageous, attractive character, the perfect image of a hero. Myers uses quite the opposite of these elements to subvert the stereotype of a hero, yet still make his characters heroic. Through the mixture of quirky attributes and unpleasant characteristics, Myers invents a new kind of character that stretches the definition of antihero. Myers writes his characters as unattractive, goofy, clumsy, shy, oddball, gross, or creepy, yet they are just as successful in their role as the hero. He isn’t afraid to introduce strong female roles into his writing with Cassandra in Wayne’s World and Vanessa Kensington in Austin Powers. Females that display strength, confidence, and power while remaining feminine are key players in Mike Myers comedy writing while his male leads are unattractive heroes. Later comedic films created by former SNL cast members adopt the unattractive hero as a central character, showing the influence Myers had on his peers. Films like The Coneheads and A Night at the Roxbury grew from Myers lead in the genre with other SNL alumni at the helm. This presentation will examine the particular construction of the main characters of Wayne’s World and Austin Powers, in the films with the same titles, as heroes within the framework of comedy. It will also attempt to examine how Myers subverts the conventions of an ideal hero to create a source of comedy for his movies.

Spencer Robb, Dixie State University Communications Ebola is making history as one of the most feared viruses in the world. It has demonstrated its power by infecting over 14,000 people and continues to spread. It has caused cities in Africa, filled with thousands of people, to become desolate. As death rates have increased, other countries outside of Africa have been affected as well. This pandemic has driven many people and researchers frantically searching for a cure, a vaccine, or preventative implementation that will decrease this sense of urgency. Perhaps even more dangerous than the physical spread of Ebola within these non-African nations, is the anxiety caused by the uncertainty and fear of a possible pandemic. Indeed, the more any society is exposed through numerous media channels to outbreak concerns, the more fear, for that group, becomes a self-perpetuating force. This presentation, therefore, will utilize the extant academic and journalistic resources to examine two pathways of inquiry: the first is how the Center for Disease Control (CDC) provides service to those who have been infected with Ebola virus as well as the evaluation of steps used to prevent more infections. The second is how Uncertainty Management Theory can provide potential strategies for mitigating fear and anxiety surrounding Ebola by explaining that with more information a situation can go in one of three directions — reduce, maintain, or increase uncertainty. According to this theory, we can better gauge our information and fear prerogatives and formulate better protocols as a result.

Hayley Ford, Kristen Wilding and Matt Schinn, Brigham Young University Engineering Therapeutic proteins are specially engineered proteins used to treat many large profile diseases. Such diseases include cancer, diabetes, hepatitis B/C, hemophilia, multiple sclerosis, and anemia. The use of these proteins is specific and highly successful and the demand for these proteins in rapidly increasing. One of the largest problems with the use of therapeutic proteins is the cost of making them. The cost of producing these proteins amounts to hundreds of billions of US dollars every year. There is a growing need to find better, faster, and cheaper ways to create them. As specific therapeutic proteins are coming off patent, research labs are able to explore the processes of making these drugs that have become such a large part of the pharmaceutical industry. Here we report the use of cell-free synthesis as a more cost-effective way to produce these therapeutic proteins. Cell-free protein synthesis is faster and allows for direct manipulation and control of the protein creating environment. Cell-free synthesis can produce proteins in a matter of days as opposed to the weeks it takes to produce them in vivo. The increased manipulation and control of the environment that comes with cell-free synthesis allows improved accuracy in creating the desired proteins and is more adaptable to changes if they need to be made.

Jordan Eatough, Jeremy Struk, Andrew Priest, Brady Vance, Brielle Woolsey, Steven Balls, Camille

Adam Herron, Jared Thomas, Shawn Coleman, Douglas Spearot, and Eric Homer, Brigham Young University Engineering For many years, x-ray diffraction and electron diffraction have served as effective means to understand and classify the molecular structure of many materials. Diffraction, as a physical phenomenon, is well known and theoretical diffraction simulation is relatively simple for perfect crystalline structures of known orientation. Prior methods of diffraction simulation, however, are insufficient to predict experimental diffraction patterns of unknown crystal structures or of crystal structures with high defect density. Recent advancements in computing capability and development of atomistic simulation software have greatly enhanced our ability to predict material properties and behaviors under various conditions. Atomistic simulation has become an extremely useful tool in the analysis of dynamic chemical and mechanical systems. It can only be truly effective, however, when it models a real-world application, can be interpreted coherently, and can accurately predict future conditions. Thus, we are developing new tools that bridge the gap between electron diffraction through real materials and simulated diffraction through atomistic simulations. We present a method of generating Kikuchi Diffraction Patterns from atomistic simulation data with no a priori knowledge of the crystal structure or crystallographic orientation. Our research was inspired by the recent work of Coleman et. al. 2013 and builds on their methods of calculating diffraction intensity at discrete locations in the reciprocal domain. We improve on their method by introducing an integration of the structure factor to ensure complete capture of diffraction intensity peaks while maintaining a relatively low density of sample points. This allows us to significantly reduce the required computation time on the analysis of atomistic simulation data. We use this diffraction data to generate simulated Kikuchi Diffraction Patterns.

Theo Stoddard-Bennett and Steven Christiansen, Brigham Young University Engineering Damage to the human retina is often irreversible and so currently there are no established treatments of diseases such as dry age related macular degeneration (AMD). Dry AMD results in a loss of sight because of cell death in the macula, a centralized part of the retina which contains a high concentration of photoreceptor cells. One possible treatment would be to limit the rate of cell death within the macula, however this is not a comprehensive solution. Rather, regeneration of the photoreceptors within the retina is necessary to restore sight. In current research, Müller glia cells, a major glial component of the retina, can potentially be used as sources for photoreceptor regeneration in order to combat dry AMD due to their homeostatic regulation of retinal injury. Directed reprogramming would occur through a five step process. The Müller glia would need to undergo de-differentiation to Müller glia-derived progenitor cells (MGPCs), proliferation of MGPCs, migration of MGPCs, neuronal differentiation, and integration in order to generate retinal neurons. Müller cells can be isolated and cultured by dissociating retinal tissue in optimal media. Here we present the dissection and dissociation of rat retinal tissue to obtain purified proliferating Müller cell cultures. Our lab has tracked and modelled the rates of proliferation and phenotypically characterized the stages of proliferation. Using immunofluorescence and PCR tests to confirm purity, we will then expect to run a series of assays to identify growth factors, Wnt signals and cytokines to test the effects of retinal extracellular matrix proteins on Müller cell de-differentiation to MGPCs. The focus of our current research is the identification of reprogramming mechanisms that may possess beneficial data leading to both unique strategies for promoting retinal regeneration in mammals and clinical applications for those living with dry AMD.

Dan Barfuss, Brigham Young University Engineering Shale oil has long been seen as a source of energy that can be incorporated into existing infrastructure. It consists of kerogen (or organic matrix) bound to inorganic rock. This kerogen can be released as an oil-like substance by heating it up to high temperatures without the presence of oxygen (i.e., pyrolysis). Due to advances in NMR (Nuclear Magnetic Resonance) we were able to make an accurate structural based model that can predict the relative tar and light gas yields[1]. We modified the Chemical Percolation Devolatilization Model (CPD) of coal to fit with the more aliphatic nature of oil shale. The CPD model describes the aromatic regions as clusters and aliphatic regions as bridges. As these bridges are broken the model releases groups of clusters that will form tar. In coal the bridge breaking gives off light gases, whereas in shale oil the bridges are much heavier and mostly form tar. We built two models that accounted for this. We also used the composition of the tar and the gas found by Fletcher et. al. [2] to predict what elements would be left and the aromaticity of the carbons. We found that throughout the reaction new aromatic regions were formed. With information from this model,- we are able to better predict the products of oil shale pyrolysis, and describe what happens chemically.

Takami Kowalski, Warren Robison, Anton Bowden, and Brian Jensen, Brigham Young University Engineering Using a coronary stent to expand a blocked blood vessel as a way to treat coronary heart disease has proved effective in the past. However, there are risks, such as thrombosis, that are a natural side effect of inserting a foreign object into the body. Creating a stent out of a hemocompatible material such as carbon-infiltrated carbon nanotubes could potentially resolve these issues and also make unnecessary treatments such as dual antiplatelet therapy as a way of decreasing the risk of adverse side effects. Previous research done in this lab has shown that carbon-infiltrated carbon nanotubes can be grown in a pattern defined by photolithography on a planar surface. The present work demonstrates preliminary results from patterning a flat, flexible substrate and rolling it into a cylindrical shape before growing carbon-infiltrated carbon nanotubes as a way to fabricate cylindrical stents, fulfilling all necessary specifications for a stent with the added benefit of hemocompatibility. We also demonstrate growth on curved substrates and explore process parameters for achieving good-quality CNT forests.

Benjamin Buttars, Jeffrey Nielson, Spencer Baker, Jonathon Thibaudeau, Angela Nakalembe, Tim

Steven Stanley, Brigham Young University Engineering The genetic code has long been restricted to a set of 20 fundamental building blocks called amino acids. Recent research has expanded the genetic code through unnatural amino acids (uAA), thus adding enormous possibilities to the potential chemistries of proteins. Because nature is highly selective in the protein translation process, it has proven extremely difficult to successfully insert multiple uAAs simultaneously. The incorporation of an uAA with in vitro methods typically relies on the use of the amber stop codon as a mutated insertion site. A stop codon placed at the middle of a gene can code for either the uAA or termination, thus, protein synthesis may often terminate prematurely instead of inserting the desired uAA. This inefficiency inhibits the possibility of inserting multiple uAAs simultaneously. We propose a novel method that will allow for multiple uAAs to be inserted simultaneously. Our method involves isolating a minimal set of tRNA for in vitro protein synthesis, allowing for uAA insertion to occur at codons other than the amber stop codon. My work has focused on the production of 4 versions of uAA-tRNA synthetase, a protein that charges tRNA with the uAA. We are currently growing these 4 different proteins in bulk and testing their activity. We will test them for compatibility, confirming that they do not interfere with one another and other synthetases native to our in vitro protein synthesis system. These uAA-tRNA synthetases, in conjunction with specialized tRNA, will provide the basis to efficiently incorporate multiple uAA simultaneously. The success of this project will have many practical applications ranging from new therapeutics to new methods of medical diagnosis.

Logan Carter, Southern Utah University Education Many students learn best from hands on activities. I myself have had several experiences that have led to deeper understanding and growth. I have participated in many scientific extra-curricular activities such as the SUU Science and Engineering fair, The International Science and Engineering fair, and NCSSSMST conventions. Throughout all of these I gained a deeper understanding of scientific concepts. To test the hypothesis that learning in elementary school students may be affected positively by experiential learning because it engages them in whole brain learning, I have designed an engaged learning set of activities. I will test these activities on approximately twenty students between the ages of nine and eleven at North Elementary School. I will measure how students respond to a set of experiential learning activities through pre and post-activity assessments. For example, we will learn about parental care in amphibians, specifically Alytes spp. -the midwife toads. Male midwife toads wrap the fertilized eggs on their legg’s and care for them until they hatch. The activity would be to split the students into groups and making one group gives the other group the eggs (balloons on a string) to the other group to simulate caring for them. That group would then race to a finish line with the balloons around their legs, making sure not to break any of the eggs. The students with the most unbroken eggs would be awarded a prize for parental care. Experiential learning is an effective way for students to gain knowledge.

Erika Longino, University of Utah Education DesignbuildBLUFF is a development program wherein graduate architecture students apply custom designing and construction skills to make homes for Navajo families and individuals in need. 39% of the Utah reservation is under the poverty line and the whole Colorado Plateau is in an ecologically vulnerable position. There is clear evidence that water tables are dropping and native biodiversity is suffering because of anthropogenic climate change. Therefore, the human communities in the region are especially needful of proper infrastructure for sustainable development. DesignbuildBLUFF provides diverse services to many groups. The aim of this investigation is to map a framework for sustainable expansion and overall systematic improvement. The investigation will eventually produce a document outlining the inputs and outputs of DesignbuildBLUFF, environmentally and socially. Ecological components like soil quality, solar potential, rainfall, and plant inventory are noted and analyzed quantitatively. Social impacts are gathered using interviews and census data. The data is pulled together in a cohesive, aesthetic map and used to plan DesignbuildBLUFF’s trajectory. The document will provide a clear framework for acknowledging and eliminating unnecessary features, reducing the ecological footprint of the organization, and improving important social relations. This document is not a definitive “master plan”, rather it provides the necessary data and suggestions for growth that take into account whole systems thinking.

Shayla Miller, Dixie State University Education My research focuses on the positive side effects of using models and representations to facilitate student learning in the classroom. The science lesson was taught and recorded to a class of thirty students. The students made visual models/representations of their knowledge about ecosystems from the lesson taught. Test scores were improved, and the information was proven to stay in long term memory according to end of level testing.

Lauren Ezzell and Lauryn Chapman, Snow College Education Most secondary level public schools have school counselors. However, the actual responsibilities of counselors are seen differently by people. There are studies demonstrating the perspective of principals’, teachers’, and counselors’. Yet, little attention is paid to the parents’ perspective and no attention to the students’. That’s why the research, Perceptions of School Counselor’s Responsibilities, is important. Surveys were distributed to high school students and parents throughout Utah. Surveys asked what were the counselors’ three main jobs. The majority of responses were: scheduling, ACT prep, graduation requirements. Participants were asked if there were jobs their counselor was supposed to do, but wasn’t, and what service they would like them to provide. Data was analyzed using SPSS to determine correlative trends then compared to the Utah Model for Comprehensive Guidance and Counseling. College students were also surveyed to determine their understanding of the counselors’ job. Students were asked two questions: What is the job of the college counselor? and What is the job of the college advisor? Responses were coded into five categories: knew job of counselor, knew job of advisor, unaware of difference, knew both, and didn’t know either. Results show when problems arise students confide in teachers instead of counselors, although parents thought differently. When asked what job counselors should do, the majority said emotional/wellness counseling. Results compared to the Utah Model, showed ‘Guidance Curriculum’ was given 3% (students) and 2% (parents) but the state gives 22%. Responses of college students showed only 5% knew the jobs of both the counselor and advisor, and only 15.1% of students knew the job of counselor. As a result, individuals are unsure of the school counselors’ job, which leads to the counselors being viewed as glorified registrars. This research could have lasting impacts on not only schools, but also individual students.

James Newton, Scott Anjewierden, and Sasha Luks-Morgan, University of Utah Engineering Oxytocin (OXT), a neuromodulatory peptide produced by the hypothalamus, is involved in a variety of physiological and behavioral phenomena. Exogenous OXT and drugs that mimic OXT signaling are potential treatments of a number of neurological disorders. The canonical mechanisms of OXT function are neuroendocrine in nature, as the peptide is released into circulation through the neurohypophysis. However, OXT has also been shown to exert some of its effects through direct synaptic release within the central nervous system. Using the larval zebrafish as a model, we seek to identify targets of these directly projecting OXT neurons and study what role they play in the modulation of behavior. Critical to this analysis are computer programs which enable precise quantification of anxiety, social behavior, and reward learning. Our custom-written software automatically identifies and tracks free- swimming fish, using measured positions over time to evaluate behavior in a variety of paradigms. In combination with molecular, cellular, and optogenetic manipulation of OXT networks, this project will allow a fuller understanding of the relationship between these neurons and behavior.

Conner Earl, Brigham Young University Engineering The emerging field of Cell-free protein synthesis enables the efficient production of complex proteins for a number of exciting applications such as medicines that better interact with the body, vaccines, antibodies, and renewable, sustainable biocatalysts. However, progress is hampered by high costs and low yields of necessary proteins. This project is designed to improve protein yields and drive down costs by studying techniques of optimization of protein yields in Cell-Free protein synthesis. Our main area of focus is the inhibition of naturally occurring ribonucleases (RNAses) which are enzymes that degrade essential elements for protein synthesis- specifically, the mRNA used to transcribe protien. One of the techniques we intend to use for inhibition of these RNAses is by complexing the RNAse with an appropriate RNAse inhibitor protein thus limiting or eliminating its function of degrading mRNA. The aims of this research project is to: (1) Identify appropriate RNAse inhibitors (2) Design and synthesize inhibitor genes (3) Express, purify and assay RNAse inhibitors (4) Improve Cell-free protein synthesis yields utilizing RNAse inhibitors for analysis of activity and effectiveness as well as the enhancement of cell-free protein synthesis yields. Accomplishing these goals will result in more efficient systems and more accurate analysis that may lead to cheaper, more readily available vaccines and pharmaceuticals produced through Cell-free protein synthesis.

Nandini Deo, University of Utah Engineering Air quality in the United States has come under scrutiny in recent years. Many pollutants are trapped in the air we breathe in the form of photochemical smog. The aim of this research is to aid the breakdown of these pollutants. Peroxyacetyl Nitrate (PAN) is a predominant smog species; the research conducted aims to decompose this molecule and capture the resulting particles using the photocatalytic properties of Titanium Dioxide Nano tubes. The research conducted thus far has focused on the following questions:What molecules does the thermal decomposition of PAN produce? Is there a metal substrate to attach to TiO2 Nano-materials that aids the breakdown of PAN and its decomposition products? Can a sustainable process/device be identified to functionalize these materials? Literature research shows that PAN thermally decomposes into CO_2, NO_2, methyl nitrate, and formaldehyde. Methyl Nitrate and CO_2 may be eliminated using specific experimental conditions. Hence, it can be determined that the substrate attached to TiO2 must decompose PAN, NO_2 and formaldehyde. Using the molecular modeling programs Avogadro and MOPAC, 50 metals were optimized in relation to Formaldehyde, NO_2, and PAN. To find each metal’s reactivity to each target compound, HOMO/LUMO (Highest Occupied Molecular Orbital/Lowest Occupied Molecular Orbital) energies were calculated and used to find the common reactive metals between the target compounds: Cobalt, Silver, Iridium, and Niobium. To test whether the most complex product of the PAN decomposition (Formaldehyde) will break down, a device was created using a 3-D printer and Cobalt functionalized nanotubes. Pure formaldehyde, a blank sample (no tubes), and a sample with functionalized tubes were run through the device in the form of vapor, in front of a solar simulator. The captured vapor’s GC/MS results show an almost complete breakdown of Formaldehyde with the use of the device containing the functionalized tubes.

Carlton Reininger and John Salmon, Brigham Young University Engineering Electric Vehicles (EV) are a rising alternative to standard combustion vehicles because of their energy cost savings and reduced carbon emissions. However, EVs come with limitations such as limited driving range and potentially long recharge times. The purpose of this study is to determine the feasibility of implementing an electric vehicle system into an urban environment. Using data provided by the New York City Taxi and Limousine Commission, models are developed and generated to simulate driver shifts and analyze system level impacts from EVs on driver behavior. The models evaluate the number of charge events over the course of a shift and calculate the potential revenue lost to missed fares during charge intervals. Across the system, the results indicate that for a majority of NYC taxi drivers, EVs can be implemented without significant changes in driver behavior, while providing an economic and environmental advantage over current combustion vehicles. These preliminary findings can be used to support implementing such a system in urban environments and these models could be used as a template toward analyzing EV taxi potential in other cities.

Scott Anjewierden, James Newton and Joshua Barrios, University of Utah Engineering Organisms in their natural environment are constantly presented with sensory stimuli. These stimuli must be filtered by the brain to select an appropriate behavioral response. A significant example of this filtering process is audiomotor prepulse inhibition (PPI). In PPI, the startle response to a loud noise is suppressed by a preceding stimulus of lower intensity. This ability to optimize behavior in response to environmental context is an essential brain function. Defects in PPI are associated with neurological disorders such as obsessive- compulsive disorder, Tourette syndrome, and schizophrenia. This project demonstrates the development of new software to analyze swim kinematics in a restrained, larval zebrafish model of PPI. Our programs automatically extract several kinematic parameters from image sequences of behaving animals and use them to classify behavior into one of three, stereotyped categories. Correct classification is reported in 96.32% of trials (n = 162). This automated analysis will now permit a more robust study of PPI in these animals, where the brain’s experimental accessibility will allow us to discover the cellular bases of sensory filtering.

Robert Erickson, Utah Valley University Life Sciences Although the insect fauna of the Colorado Plateau region are somewhat well known, our specific understanding of the arthropod biodiversity in Capitol Reef National Park has been sparse. Objective: From the multiple insect surveys conducted in Capitol Reef National Park we intend to catalog the arthropod biodiversity into a species list. Methods: In addition to the previous collecting trips, we carried out collection efforts this past summer (2014). We used the Utah Valley University Capitol Reef Field Station as our home base. General insect collecting efforts were conducted around the field station and the nearby Pleasant Creek, near the public campgrounds, along trails, and in several other locations in the southern portion of the park. Additionally, we performed night collecting with a mercury vapor lamp trap on the nights we were in the park and utilized stationary malaise and aquatic larvae traps. The collected specimens were curated using methods of pinning, spreading, labeling, identifying, photographing, and organizing the insects. Results: The collections contributed to an increased understanding of the parks insect diversity and resulted in a curated natural history museum collection. A species list will be made available for the records of Capitol Reef National Park. More than 3000 specimens have been collected within the park. Furthermore, a booklet of the common insects for the park is in the process of being created in order to serve as an educational tool for visitors to the park and field station.

Jenny Pattison, Brigham Young University Life Sciences Sensory protein kinases are essential in the phosphorylation of many protein substrates, allowing them to control several metabolic functions and maintain cellular homeostasis. PAS kinase is a sensory protein kinase that is highly conserved and plays a crucial role in glucose homeostasis, however little is known about the molecular mechanisms behind its function. UGP1 is the only well-characterized substrate of PAS kinase, and its phosphorylation diverts glucose away from storage and towards cell wall biosynthesis. We have recently discovered another key substrate of PAS kinase that affects glucose metabolism in the cell, Centromere binding factor 1 (Cbf1). Cbf1 regulates genes involved in respiration, and we have shown that the phosphorylation of Cbf1 by PAS kinase inhibits Cbf1, decreasing respiration in yeast cells. We hypothesize that this is due to a decrease in mitochondrial mass in cbf1 deficient yeast. Further characterizing the effects of PAS kinase on Cbf1 will give further insight into how cells regulate their central metabolic functions, including respiration.

Chase Barker, Utah Valley University Life Sciences Central Research Question:

Stephan Maman and Holden Wagstaff, Southern Utah University Life Sciences Most plant species produce chemical compounds called secondary metabolites that enhance fitness in a variety of ways. Many of these compounds are also physiologically active in vertebrates and have widespread medicinal uses. The most ubiquitous secondary metabolites are the terpenoids, many of which cause vasodilation of the aorta and mesenteric arteries. In this study, we examined the vasoactive effects of the essential oil of Umbellularia californica, which contains the terpenoid umbellulone. Oil obtained via steam distillation using aerial portions of U. californica was applied directly to cutaneous arterioles of frogs. Arteriole diameter was monitored both before and after oil application by video microscopy. Within seconds of application, the oil caused significant vasoconstriction that persisted until the oil was washed off. Our control, medical grade sesame oil, caused no observable effects when applied using the same protocols. These results are opposite to the vasodilatory effects of terpenoids on aortic rings and mesenteric arteries. This suggests that the vasoactive effects of umbellulone are different from other terpenoids, that the vasoactive effects of terpenoids differ depending on blood vessel type, or that application of the complete essential oil affects vasculature differently than application of the isolated terpenoid.

Kylynn Parker, University of Utah Life Sciences Red Butte Canyon (RBC) is a Research Natural Area administered by the US Forest Service in Salt Lake City, Utah. RBC is an undisturbed area and a haven for all types of birds. Most of the avian species found in this area are migratory, and either pass through or breed in the area. The overall aim of this project is to determine if there have been any notable changes in populations of species in the area over the past 22 years. The research question that is covered in this summary are the following: has the density and relative abundance of the top five most commonly detected avian species in Red Butte Canyon notably changed through time in Transect 1? Data was collected by Mark Leppert, PhD and Sherwood Casjens, PhD of the University of Utah. They recorded the number and species of birds that were both seen and heard in 10 different transects within RBC over the past 22 years (1991-2013) and 457 survey days. In 2013 and 2014, I compiled and entered all of the data into a database with the guidance of the researchers. For analysis, I focused on the five most commonly detected species in Transect 1. These species are Black-Capped Chickadee (Poecile atricapilla), Warbling Vireo (Vireo gilvus), Yellow Warbler (Dendroica petechia), American Robin (Turdus migratorius), and Lazuli Bunting (Passerina amoena). Detection trends were found by graphing the number of individual birds seen or heard in Transect 1 over the days since surveys began in 1991 and statistical evidence was found showing significant changes in species population size of these five most commonly detected species, especially in the case of the American Robin which exhibits a decline in detections in recent years.

Michael Packer, Utah Valley University Life Sciences Dipsosaurus dorsalis is a desert dwelling iguana species geographically distributed throughout the south-western United States, northern Mexico, and the Baja Peninsula. Throughout the Baja Peninsula, multiple species of herpetofauna exhibit a distinct north-south division, both morphologically and genetically, with no presence of any physical barriers. The goal of this study was to examine how the genetic structure of D. dorsalis has been influenced by past geological events, and whether this species exhibits a similar north-south division on the Baja peninsula. Additionally, this study aimed to see if the current taxonomy of Dipsosaurus is reflective of the evolutionary relationships across its entire geographic range. 100 tissue samples of D. dorsalis were collected across the species geographic range. DNA extraction from collected tissue samples and sequencing of nuclear loci MLH3 (~900bp), NT3 (489bp), as well as mitochondrial loci ND4 (~900bp) were performed to examine the distribution of genetic variation in D. dorsalis. The collected data was then used to construct phylogenetic trees for each locus, comparing individuals of Dipsosaurus to the geologic history of its geographic distribution. Although shallow, a maximum likelihood tree of the ND4 mitochondrial gene shows the phylogeographic separation of three distinct clades. The results do not support the designation of Dipsosaurus catalinensis as currently defined.

Daniel Barnett, Brigham Young University Life Sciences Approximately one-third of US adults have metabolic disease, increasing their risk for diabetes, cancers and neurodegenerative disease (www.ADA.org). At the heart of these diseases are imbalances in the cellular redox state. The cofactor NAD(P), commonly known as niacin, is required for over 300 essential reactions in the cell and is largely responsible for the cellular redox state. NAD kinase regulates the NAD to NADP ratio, an important ratio for controlling cellular redox state and central metabolism. Herein we provide evidence that PAS kinase, a nutrient sensing kinase required for glucose homeostasis, phosphorylates NAD kinase. We are currently investigating the effect of this phosphorylation on the function of NAD kinase both in vitro and in vivo by measuring NAD kinase activity and associated phenotypes. This research will increase our understanding of how cells regulate central metabolism. In addition, because PAS kinase is a nonessential protein, it may prove to be an invaluable treatment target for regulating NAD(P) levels and controlling cellular redox state. This may lead to therapeutic targets for cancer and metabolic diseases such as diabetes.

Brent Knoblauch and Marty Larsen, Dixie State University Life Sciences Chytrid fungus (Batrachochytrium dendrobatidis) is one of the major hypothesized theories behind global amphibian decline. Canyon Tree frogs (Hyla arenicolor) found in Zion National Park have been found to contain infected individuals, however population sizes have grown despite infection with the usually deadly fungus. The present study sought to demonstrate a correlation between skin temperatures and fungal proliferation, and investigated what enabled Hyla arenicolor to survive and reproduce with chytrid present. Ten frogs were sampled with sterile swabs from each of seven different study areas found in Zion National Park. Along with each swab a temperature was taken via infrared thermometer and recorded. DNA was also extracted from the swabs, and Batrachochytrium dendrobatidis- specific primers were used in a touchdown PCR protocol to determine infection rates. Swabs from specimens with higher temperatures were expected to display lower instances of infection. This would establish for the first time a clear demonstration that high skin temperatures were destroying the fungus in infected individuals in the wild.

Stephanie Bartlett, Utah Valley University Life Sciences Our project investigates the phylogenetic relationships of the superfamily Ephemeroidea + Behningiidae. Found in waters worldwide, burrowing families are unique in that they have mandibular tusks that allow them to tunnel in the silt or gravel of riverbeds. Surprisingly, even without the mandibular tusks necessary for tunnel construction, the Behningiidae family is still found within these burrows as nymphs. Because the Behningiidae don’t have tusks, morphological research has lead to a phylogenetic classification of this family that our genomic investigation ultimately disputes. To begin this investigation, mayfly specimens were collected worldwide and prepared for DNA extraction. For each specimen genes were amplified via polymerase chain reaction and visualized on an agarose gel, before being sequenced and analyzed. The specific genes targeted for this analysis include; 12s mitochondrial rDNA, 16s mitochondrial rDNA, 18s nuclear rDNA, 28s nuclear rDNA, H3 nuclear protein coding, and CO1 mitochondrial protein coding. Datasets were supplemented with sequences acquired from Genbank. The ingroup consisted of approximately 30 samples. Phylogenetic relationships were estimated using Maximum Parsimony, Maximum Likelihood, and Baysian methods. We constructed phylogenetic relationships of burrowing mayflies using molecular DNA data analysis, when compared to morphological analysis we identified some important classification differences. As a result of our findings, we propose an alternative explanation for the evolution of mandibular tusks in burrowing mayflies (Ephemeroptera). The data support that burrowing mayflies first evolved tusks. Within the family Behningiidae, tusks were lost, while the burrowing lifestyle (i.e., living in a burrow to filter feed) was retained. This study represents the largest analysis to date for these insects and strongly supports the evolutionary trend of a gain and a loss of mandibular tusks during their evolution.

Jacob Welch, Brigham Young University Life Sciences Learning and memory occur due to adaptive brain changes in response to our environment. These changes are mediated by synaptic plasticity, particularly within the hippocampus. Plasticity can either strengthen or weaken synapses, known as long-term potentiation (LTP) or long-term depression (LTD) respectively. While many forms of plasticity are NMDA-dependent, recently endocannabinoids were identified to mediate several new forms of hippocampal synaptic plasticity through the CB1 and TRPV1 receptors. However, research has demonstrated a non-CB1/TRPV1-dependent endocannabinoid synaptic plasticity in the hippocampus. Several potential candidate receptors that bind the endocannabinoid anandamide have been identified. These are among the orphan G-protein coupled receptors (GPRs) whose distribution in the brain and/or function is less well known. GPR55 is of particular interest as it activates second messenger systems. Using quantitative RT-PCR, electrophysiological and memory behavioral tasks we examined hippocampal GPR55 expression and function. GPR55 is expressed in hippocampus of both rats and mice. Cellular expression is currently being examined and appears to be rare in interneurons and more likely expressed by pyramidal cells. Interestingly, application of the GPR55 agonist LPI (2 μM) to wild-type mice demonstrates a significant enhancement of LTP in brain slices. This LPI effect was not noted in GPR55 knock-out (KO) mice, which exhibit significantly (p < 0.05) smaller LTP (146%) than wildtype (WT) (181%). GPR55 also appears to increase release probability (Sylantyev et al., PNAS, 2013), denoting a presynaptic role. Paired-pulse ratios are now being analyzed between GPR55 KO and WT mice to confirm this finding; however we did not note a change in EPSCs in CA1 in response to 2μM LPI. These data suggest GPR55 is expressed and physiologically relevant in the hippocampus. Because enhanced LTP is usually associated with better memory performance in rodents, this provides a potential target to enhance the cellular mechanism associated with memory formation.

Bryce Taylor and Ian Kesler, Southern Utah University Life Sciences Green infrastructure is the use of natural processes to manage stormwater runoff and has many positive effects on evaporative cooling, building energy demand, and wildlife habitat. Very little green infrastructure research has been conducted in arid environments such as Southern Utah. The scope of this research project is to establish a complete water budget of the green roof using a lysimeter (an acrylic box resting on a high-resolution weighing scale). The water budget will help in determining whether the cost of irrigating the green roof is off- set by the potential benefits to biodiversity and decreased energy demands.We have constructed a working lysimeter and will collect the required data by using: a high resolution scale, a tipping bucket to measure drainage, two soil moisture and temperature sensors and weather data from the campus weather station. A collection of mixed Sedum species vegetation will be planted in the lysimeter using a special green roof media to accurately simulate a greenroof environment. Both pitfall (used to capture grounded insects) and combination traps (used to capture airborne insects) will be used to measure the biodiversity. Data collection will begin during the winter of 2014 and continue indefinitely.

Danny Ferguson, Utah Valley University Life Sciences This research examined the acceptance of evolution for introductory Biology students and the reasons why they don’t accept evolution, accept evolution, and why they change their minds over the semester. Previous studies examined student’s observations and knowledge of the evolutionary theory and found that the degree of conflict students perceived between religion and science was negatively correlated with their knowledge of evolution. Objective: The objective of this research was to better understand the student’s views of evolution and its integration into their worldly and religious views. Methods: We will conduct interviews with general Biology students in order to better understand the reasons why they don’t accept evolution, accept evolution, and why they change their minds over the semester. The interview questions are designed to investigate, in more detail than the previous surveys, the opinions of evolution and how they change over the course of the semester. The recordings will be transcribed and quantified by binning answers into categories. Given high % of students are LDS, we will ask a few additional questions to this portion of the population. Results: We found that as students knowledge of evolution increased, their conflict with religion decreased. The data demonstrate that there are three main reasons for this trend: 1) Evidence convinces the students to accept evolution; 2) Particularly for LDS students, knowledge of the official position of the Church, enables them to allow evolution to be a correct process in nature; and 3) the instructor as a role model (believer yet accepts evolution) can have a large influence.

Jake Loveless, Utah Valley University Life Sciences Macroinvertebrate assemblage composition was assessed in two perennial streams, Pleasant Creek, and Sulphur Creek in Capitol Reef National Park, Utah, during the summer of 2014. Individuals collected were identified to genus. This information was used to compare assemblage composition, nutrient availability, and water quality between the two streams. Five collection trips were made (May 27, June 10,17, July 1,15). Sampling was conducted randomly using mesh kick nets with four samples being collected per site, per trip. Heavy rains caused flash flooding on July 15 prohibiting sampling, so a total of sixteen samples per site were taken. Samples were stored in 90% isopropyl alcohol until they could besorted and identified using a dissecting microscope. Water quality estimates were determined by taking the weighted average of the tolerance values, and the final classification was made using the family-level biotic index. Pleasant Creek showed the highest number of taxa present, fourteen, compared to six in Sulphur Creek. Both streams followed the predictions of the River Continuum Concept consisting of collector-dominated functional feeding groups, with Pleasant Creek showing a higher percentage of predators 35.7% to 16.6%. Tolerance values of collected taxa were used to estimate organic pollution indicating good water quality in Pleasant Creek, and fairly poor water quality in Sulphur Creek. The differences in the macroinvertebrate assemblage composition in this study were likely multi-causal. Stream size is a major factor influencing the structure of macroinvertebrate assemblages; in general, as stream size increases, more taxa are added. Pleasant Creek is a much larger stream with a well-established channel, while Sulphur Creek is shallow with depths never exceeding fifteen centimeters. The shallow channel of Sulphur Creek also made it prone to frequent flooding. Isolated floods have little impact on macroinvertebrate communities due to availability of aerial adults nearby to recolonize rapidly, however, frequent flooding may have long-term effects through extirpation of taxa with high mortality. The difference in water quality was the most surprising finding in this study. The fairly poor water quality of Sulphur Creek may also be a factor in the lack of genera found there. Organic pollution effects primary productivity in streams, and while primary productivity was not measured in this study, visual observations found very little algae or aquatic vegetation at Sulphur Creek.

Anne Thomas and Richard Gill, Brigham Young University Life Sciences Providing water for expanding urban communities in the western United States is a growing concern for city planners and governmental agencies. Landscaping can claim up to 50% of the urban water budget but also has the most potential for water conservation. Landscape water use is highly variable, however, because of species-specific differences in tree water use and because of decision-making by city planners and residents in maintaining trees. The objective of this study is to improve our ability to predict urban forest water use by identifying differences in tree basal area and diversity between neighborhoods that have arisen at different periods of development in Heber Valley, Utah. We classified neighborhoods as established, exurban (rural housing), commercial, or new tract based on age, location, and lot size. We performed a stratified random survey with twenty lots in each category and collected diameter and species data for each tree in the lot. Some of the patterns we observed were easily anticipated, such as higher basal area per hectare in the older, established neighborhoods relative to newer tract housing. Surprisingly, the number of individual trees per hectare in tract and established neighborhoods is very similar. Perhaps of more interest is the low species richness of tract housing compared to exurban communities. Because exurban communities are being replaced by tract housing there is evidence that tree diversity will be lost. Another important aspect of community structure in urban forests is the ratio of conifers to broadleaf trees because of fundamental differences in water use patterns. Conifers comprised twenty-five percent of the basal area in exurban and thirty-five percent in established neighborhoods, as opposed to five percent in tract. Our data suggest that tree diversity is likely to decrease while water demand is likely to increase with changes in urban forests in the coming decade.

Katherine Harris, Brady Evans and Thomas Andros, Brigham Young University Life Sciences Metabolic diseases, such as obesity and diabetes, have become endemic and the need for better treatments is rising. Mutations in PAS kinase, a recently discovered sensory kinase, have been shown to cause Maturity Onset Diabetes of the Young (MODY) in humans (Semplici et al., 2011). In addition, PAS kinase deficient mice display many phenotypes related to diabetes including resistance to weight gain, insulin insensitivity and triglyceride accumulation in response to a high-fat diet (Hao et al., 2007). Despite its importance in metabolism, little is known about the regulation of PAS kinase. PAS kinase consists of a sensory PAS domain that binds to and inhibits a protein kinase domain (Amezcua et al, 2002). We are currently engaged in several yeast genetic screens which will allow identification of regions in the full length PAS kinase that are essential for activation or for binding its substrates. The first screen is based on the finding that PAS kinase overexpression rescues a temperature-sensitive mutation in Tor2, the tor2(ts). We have isolated both point mutations and truncations in PAS kinase which alleviate the tor2(ts), suggesting they are hyperactive alleles. These mutations identify novel regions involved in PAS kinase regulation. Our second screen uses the yeast 2-hybrid to select for both point mutations and truncations that increase the ability of PAS kinase to bind its substrates. These mutations will help identify key regions of PAS kinase utilized in substrate recognition. Finally, we have identified regions of PAS kinase that are well-conserved throughout evolution and will compare these regions with the regions affected by our mutations. This study will be the first reported mutagenic analysis of PAS kinase. Analysis of these specific genetic regions will help elucidate the molecular mechanisms involved in the regulation and function of PAS kinase, a key player in the development of metabolic disease.

Scott Newton, Brigham Young University Life Sciences The ventral tegmental area (VTA) is known to controls the processing of rewarding and addictive behaviors. The VTA contains dopamine (DA) cells, which release DA to downstream targets in response to rewarding stimuli, and GABA cells, which modulate DA cell activity. Therefore, both cell types are involved in associative reward learning. Synaptic plasticity plays an important role in adaptive reward signaling within the VTA. Endocannabinoids mediate or modulate synaptic plasticity at synapses within the reward circuit. However, the source of endocannabinoids within the VTA is not well understood. Therefore, our goal was to describe the distribution of endocannabinoid biosynthetic enzyme mRNA within VTA neurons. We extracted single VTA neurons via whole cell patch clamp and used single-cell real-time quantitative PCR to identify DA and GABA neurons based on mRNA expression of cell-type specific targets. Additionally, electrophysiological properties such as action potential frequency and sag potential amplitude were examined between the two cell types. Concurrent with established observations, slower firing frequencies were observed in DAergic neurons, however overlap was identified between these two cell types. VTA neurons were then probed for endocannabinoid/ biosynthetic enzyme mRNA, such as N-acyl-phosphatidylethanolamine-specific phospholipase D (NAPE- PLD), diacylglycerol lipase α (DAGLα), and 12-lipoxygenase. We also probed for type I metabotropic glutamate receptor (mGluR) mRNA, as endocannabinoid synthesis requires mGluR activation. Our data demonstrate that endocannabinoid biosynthetic enzyme mRNA is expressed in both DAergic and GABAergic cells with concurrent expression of type I mGluRs. Next, to ensure mRNA expression was representative of protein content, slices were stained using immunohistochemistry for GAD67, DAGLα, NAPE-PLD and type I mGluRs. Positive labeling for these targets was observed in VTA neurons, supporting our RT-PCR results. Collectively, these data suggest DAergic and GABAergic cells of the VTA have the capability to produce endocannabinoids and potentially alter synaptic plasticity involved in reward and addiction.

Alex Hyer, University of Utah Life Sciences Geochemical reactions associated with the process known as serpentinization can generate copious quantities of organic carbon and chemical energy that support life, but these reactions also greatly increase the pH of serpentinization sites. High-pH environments hinder ATP production because the low proton concentrations at high pH can result in a reversed proton gradient across cell membranes. Organisms present at serpentinization sites are not well-characterized, and adaptations to their alkaliphilic lifestyle are unknown. Bacillus pseudofirmus OF4 was not isolated from serpentinization sites, but it is a well- studied alkaliphile that has several distinct adaptations for ATP production in high pH environments. Its sequence for ATP synthase, the molecular motor responsible for ATP generation in the cell, contains several distinctive mutations including an AXAXAXA motif that increases the C-ring stoichiometry, a P51XXE54XXP57 motif that creates a distinct kink in the outer helix of the C-subunit, and a V21 mutation granting rotamer freedom to E54. In this study, we search for similar mutations in metagenomic databases containing DNA sequences collected from serpentinization sites. Analysis of alignments from the programs BLAST and Exonerate indicate that V21 is present in several alignments, but P51 is found in only a single alignment from serpentinization sites. The AXAXAXA motif is non- existent in our databases, but the more common GXGXGXG motif is present with alanine replacements occurring periodically. In general, these results indicate that our DNA sequences from high-pH sites of serpentinization are more reminiscent of typical neutrophiles than of the alkaliphile B. pseudofirmus OF4. Therefore, serpentinization- adapted organisms may harbor other adaptations to high pH such as local pmf regulation. Future work will test for quantitative correlations between environmental pH measurements and the incidence of mutations in ATP synthase in order to identify novel adaptations to high pH in serpentinization-driven ecosystems.

Laine Anderson, Utah State University Life Sciences Plague is maintained in complex epizootic and enzootic transmission cycles involving rodents and their fleas. Ground squirrels, prairie dogs, woodrats and their associated fleas have been identified as essential for bacterial maintenance. Oropsylla montana is of major interest due to the fact that it is distributed throughout the western U.S. where most human plague cases occur. Evidence suggests this species is the primary vector of plague to humans. Data on the genetic variation within and among populations of potential vectors of Yersinia pestis, including O. montana, is very limited. O. montana fleas were previously collected from 35 geographically distinct field sites from Colorado and New Mexico on the east, to California and Oregon on the west. Genomic DNA was extracted and mitochondrial and nuclear genetic data was sequenced to estimate phylogenetic relationships. The data collected from these studies will expand our knowledge of natural O. montana populations. We anticipate the genetic data collected from these flea populations will provide information that will assist with understanding human plague risk. Such information will, in turn, provide potentially significant insights into the ecology and epidemiology of plague in this region and is likely to suggest new strategies for monitoring and preventing this disease.

Kyle Kener, Brigham Young University Life Sciences Diabetes is characterized by the inability to maintain a normal blood glucose level caused by decreased insulin due to β-cell loss, or decreased insulin sensitivity in the liver, muscle, and adipose tissue. While β-cell death is a hallmark of T1D, β-cells are also destroyed as T2D progresses. Death of β-cells is eventually a hallmark of both forms of diabetes. This results in decreased functional β-cell mass, which is defined by the ability to secrete insulin while maintaining β-cell number through proliferation or decreased apoptosis. To resolve the decreased β-cell level, much research is being done regarding β-cell proliferation to increase pancreatic β-cell mass. However, another important step in this process is protecting β-cells from apoptotic mediated β-cell death. The β-cell transcription factor Nkx6.1 is sufficient to induce β-cell proliferation and increase protection against apoptotic insults. The Nkx6.1 target gene VGF is critical for protection against apoptosis. Our data demonstrates that Nkx6.1 upregulates expression of c-Fos. Furthermore, we show that c- Fos is sufficient to induce expression of VGF. In addition, our data demonstrates that expression of c-Fos is sufficient to protect β-cells from apoptotic insults. Our data demonstrates that c-Fos is the link between the Nkx6.1 and VGF, and that it’s expression is sufficient to protect rat pancreatic β-cells from apoptosis.