2015 Abstracts

Benjamin Bitner, Jason Ray, Kyle Kener, and Brent Wright, Brigham Young University Life Sciences β-cell mass is lost in both major forms of diabetes. Mature β-cell have restricted proliferative capacity. Studies aimed at increasing β-cell mass frequently have the unwanted side effects of decreased glucose stimulated insulin secretion (GSIS) or increased apoptotic rates. Without functional β-cells, the body is unable to reduce elevated blood glucose, which results in hyperglycemia induced maladies. Enhanaced GSIS could be used as a treatment for diabetes. Overexpression of the β-cell transcription factor Nkx6.1 induces β-cell proliferation, enhances GSIS and protects against apoptosis. Nkx6.1 induces expression of VGF, which is necessary for enhanced GSIS. Microarray analysis of Nkx6.1 expressing primary rat islets demonstrates upregulation of c-Fos at 24 and 48 hours after adenoviral transduction. We have shown that c-Fos upregulates expression of VGF. Finally, preliminary data suggest that c-Fos modulates GSIS as demonstrated in INS-1 β-cell line, stable INS-1 knockdown cells transduced with lenti-sh-c-Fos and in primary islets. We present a model by which c-Fos is necessary for Nkx6.1 mediated enhanced GSIS by inducing expression of VGF.

Amanda Johnson, Rian Farr, and Autumn Woodfall, Dixie State University Life Sciences Autism affects approximately 1 in 68 children, and is one of the fastest growing developmental disorders in the United States. Studies suggest that autism may be a result of processes that occur during labor and delivery. Although Pitocin is widely used during labor and delivery in the United States, there is a paucity of research on the developmental outcomes of Pitocin use during labor and delivery in young children. Additionally, the components and metabolic fate that make up the compounded Pitocin are widely varied between suppliers. Samples of Pitocin were extracted to isolate the chemicals that comprised commercial available products. These were then analyzed by chemical and biological methods to determine the composition and chemical breakdown products. Understanding the components and breakdown of commercial Pitocin will help to determine the compounds potentially transferred to fetuses with Pitocin use during labor and delivery and the potential developmental outcome.

Coral Gardner and Don Long, Southern Utah University Life Sciences Irrigation of lawns and gardens in Cedar City, Utah is accomplished in two fundamentally different manners. In older neighborhoods, water is diverted from a natural stream (Coal Creek) into a series of canals and ditches for residential flood irrigation, compared to newer neighborhoods, which use sprinklers or similar devices from well water. The overall objective of this project is to better understand the chemical and biological changes that occur in irrigation and runoff waters in Cedar City. We are addressing the following three hypotheses. 1) Changes will be observed in water chemistry as surface water moves from Coal Creek through Cedar City. 2) Irrigation strategies influence water chemistry during periods of high precipitation. 3) Microbial community changes will be associated with differences in water chemistry. Water chemistry data including dissolved oxygen, conductivity, pH, alkalinity, dissolved organic matter and nitrate/nitrite were collected weekly at eight sites and during high precipitation events. Over the course of four months, there was a reduction in alkalinity levels among all sites. During high precipitation periods, nitrate was detected in newer neighborhoods. Dissolved oxygen and pH were at higher levels, while salinity and conductivity were lower in a reservoir site relative to irrigation canals. Future work will investigate bacterial community composition in Cedar City waters. We will isolate bacterial DNA from water samples and amplify the 16sRNA segment of DNA using the polymerase chain reaction. We will then correlate bacterial community composition to the water chemistry results described above.

Brianne Palmer, Utah State University Life Sciences Quaking aspen (Populus tremuloides) are declining in the interior west. Aspen are critical for the maintenance of wildlife habitat and are one of the few broadleaf trees in the western forest ecosystem. In western landscapes, it has recently been determined that a large proportion of aspen trees are triploid (three copies of each chromosome) and the remaining trees are diploid (two copies of each chromosomes). In this study we attempted to find differences in the physiology between the two cytotypes to determine future management strategies The size and density of stomata trees is likely to influence the survival of the species in water- and heat-stressed environments, since stomata control both photosynthesis rates and rates of water loss. Individuals with larger stomata or greater stomatal density may be efficient photosynthesizers but may be at risk for water loss during transpiration in environments with low precipitation and hot temperatures, such as those often seen during summers in the intermountain west. To determine if there is physiological differences between the cytotypes we measured the variation between stomatal sizes and densities between the cytotypes using cellulose acetate leaf impressions and microscope imagery. We collected leaves from twelve aspen stands (eight diploid and four triploid) representing the two cytotypes in Swan Flats and Fish Lake, Utah. From these analyses, we deduced that the variation in stomatal size and density is primarily among clones rather than among cytotypes. Further data collection and analyses will occur in the spring of 2015.

Austin Thompson and David Walton, Brigham Young University Life Sciences When we sustain a traumatic injury to the peripheral nervous system (PNS), our bodies elicit a series of responses to try to heal the acquired damage, including inflammation and repair processes. One of these responses is the increased expression of nerve growth factor receptor (NGFR), which helps to stimulate regeneration of the nerve. In a normal, healthy PNS, NGFR is rarely found. Following damage to the nerve, NGFR can be found in high levels around the damaged area. In our study, we are simulating traumatic injury to the sciatic nerve of rats in order to study the effects of regeneration after a local application of nerve growth factor (NGF). We are using both a physical crush model and a focal demyelination model to simulate the nerve injury. In the crush model, we are examining the effect of a crushed extracellular matrix (ECM) on degeneration and subsequent regeneration. In the focal demyelination model, we are investigating the effects of local demyelination with an intact ECM on degeneration and regeneration. In two additional experimental groups, we will perform an intraneural injection of NGF into the damaged sciatic nerve one week after the crush or a lysolecithin injection at the damaged site. We are examining the nerve both qualitatively using SEM and immunohistochemistry and quantitatively using electrophysiology. This allows us to understand the role of the ECM in regeneration, and its effect on the rate of regeneration. We hypothesize that the addition of NGF in combination with the increase of NGFR after injury will increase the rate of nerve regeneration. We expect regeneration to be faster in the focal demyelination model due to the presence of intact ECM than in the crush model where the ECM is damaged.

Simranvir Kaur, University of Utah Life Sciences The placenta is the major source of fetal serotonin during pregnancy, which is essential for fetal brain development. In Utah, approximately 13% of pregnant women take Selective Serotonin Reuptake Inhibitors (SSRIs) to treat depression, the use of which has been correlated to significantly lower serotonin levels in cord blood for newborns. Studies suggest association between maternal SSRI use and adverse outcomes such as preterm birth, cognitive deficit, and disruption of serotonergic systems. However the effect of SSRIs on placental gene expression, serotonin synthesis and transport in the placenta, is not known. This study evaluates the impact of maternal SSRI use on placental gene expression and levels of serotonin in the cord blood using a nested case-control observational study model. Biological samples will be collected until 20 cases (women taking SSRIs during pregnancy) and 20 appropriately matched controls have been enrolled into the study. Participants also complete an online questionnaire to measure depression and anxiety levels as well as document any medication they have taken during pregnancy. Data and sample collection for this study is still in progress. Once enough samples have been collected for batch analysis, we will complete RT-PCR and ELISA, expected in Spring 2015. Upon complete data analysis, we expect this study will help in targeting mothers who are at risk for adverse pregnancy outcomes and further provide suggestions for intervention.

Matthew Durrant and Shuqing Xu, Brigham Young University Life Sciences Researchers at the Max Planck Institute for Chemical Ecology have recently sequenced the Nicotiana attenuata (wild tobacco) genome. This genome affords researchers new opportunities to understand the evolution of this organism. One method for analyzing the evolution of specific genes in a given genome is referred to as phylostratigraphy, which makes use of large-scale BLAST sequence similarity searches. I designed a pipeline using the python programming language that implements a phylostratigraphic analysis to estimate the evolutionary age of all ~35,000 genes belonging to N. attenuata. By analyzing the large amounts of data produced by this BLAST search, each gene was assigned an estimated age through comparing the taxonomies of all other organisms that share similar protein sequences. This effectively answers the question “How old is this gene?” for each gene in the entire N. attenuata genome. Previous studies have produced microarray data that tracks the transcriptomic response of N. attenuata to an herbivore attack. By comparing the newly gathered gene evolutionary age information with this previously gathered microarray data, several new insights into the molecular signaling pathways of N. attenuata were made. It was found that at 1 hour following an herbivore attack, the transcriptome of N. attenuata is evolutionarily young, suggesting that the initial response to an herbivore attack recruits genes that have evolved more recently in the organism’s evolutionary history. At 5 hours after attack, however, there is a distinct decrease in the overall age of the N. attenuata transcriptome, suggesting that the organism is recruiting more ancient genes that are used to reconfigure the transcriptome of the organism. Beyond 5 hours, the transcriptome is once again relatively young, and it is clear that it has indeed been reconfigured to provide a more herbivore-specific defense response. This demonstrates a novel, evolutionary approach to analyzing signaling pathways in plants.

Laine Anderson, Utah State University Life Sciences Plague is maintained in complex epizootic and enzootic transmission cycles involving rodents and their fleas. Ground squirrels, prairie dogs, woodrats and their associated fleas have been identified as essential for bacterial maintenance. Oropsylla montana is of major interest due to the fact that it is distributed throughout the western U.S. where most human plague cases occur. Evidence suggests this species is the primary vector of plague to humans. Data on the genetic variation within and among populations of potential vectors of Yersinia pestis, including O. montana, is very limited. O. montana fleas were previously collected from 35 geographically distinct field sites from Colorado and New Mexico on the east, to California and Oregon on the west. Genomic DNA was extracted and mitochondrial and nuclear genetic data was sequenced to estimate phylogenetic relationships. The data collected from these studies will expand our knowledge of natural O. montana populations. We anticipate the genetic data collected from these flea populations will provide information that will assist with understanding human plague risk. Such information will, in turn, provide potentially significant insights into the ecology and epidemiology of plague in this region and is likely to suggest new strategies for monitoring and preventing this disease.

Kyle Kener, Brigham Young University Life Sciences Diabetes is characterized by the inability to maintain a normal blood glucose level caused by decreased insulin due to β-cell loss, or decreased insulin sensitivity in the liver, muscle, and adipose tissue. While β-cell death is a hallmark of T1D, β-cells are also destroyed as T2D progresses. Death of β-cells is eventually a hallmark of both forms of diabetes. This results in decreased functional β-cell mass, which is defined by the ability to secrete insulin while maintaining β-cell number through proliferation or decreased apoptosis. To resolve the decreased β-cell level, much research is being done regarding β-cell proliferation to increase pancreatic β-cell mass. However, another important step in this process is protecting β-cells from apoptotic mediated β-cell death. The β-cell transcription factor Nkx6.1 is sufficient to induce β-cell proliferation and increase protection against apoptotic insults. The Nkx6.1 target gene VGF is critical for protection against apoptosis. Our data demonstrates that Nkx6.1 upregulates expression of c-Fos. Furthermore, we show that c- Fos is sufficient to induce expression of VGF. In addition, our data demonstrates that expression of c-Fos is sufficient to protect β-cells from apoptotic insults. Our data demonstrates that c-Fos is the link between the Nkx6.1 and VGF, and that it’s expression is sufficient to protect rat pancreatic β-cells from apoptosis.

Brent Wright, Brigham Young University Life Sciences Diabetes is one of the leading causes of death among Americans and is a major health concern worldwide. Nearly one in four Americans aged 65 or older are diabetic. Type 1 and Type 2 diabetes both result in reduced functional β-cell mass, which regulates the storage and secretion of insulin. Increased functional β-cell mass could essentially cure diabetes. We have shown that Nkx6.1 overexpression induces proliferation of 2-month-old primary rat β-cells but fails to induce replication of 8-month-old primary β-cells, as measured by 3H- thymidine incorporation is age-dependent. Cell cycle activator and inhibitor mRNA levels were measured in young and aged untreated islets and islets transduced with AdCMV-BGal or AdCMV-Nkx6.1. This data demonstrated a significant increase in mRNA expression of cell cycle inhibitors p21 and p57 of the CIP/KIP family in young islets transduced with Nkx6.1. However, p21 and p57 mRNA showed no significant increase in aged islets. Cdk2 and cyclin E1 mRNA expression showed a similar trend for young and aged islets. The increased expression of Cdk2, a necessary factor for transition from G1 to S phase, could provide possible explanation for increased proliferation in young islets. Fluctuating mRNA levels of key cell cycle components in aged islets, provides a possible explanation for the decreased effectiveness of Nkx6.1 in inducing proliferation in aged islets.

Madalynne Fedoruk and Darian Carey, Dixie State University Life Sciences For at least three decades, infection with chytrid fungus (Batrachochytrium dendrobatidis) has been a major cause of population decline in amphibians worldwide. This pathogen has been found in canyon tree frogs (Hyla arenicolor) in Zion National Park; previous studies suggested that population sizes decreased in frogs infected with the fungus; but our studies indicated that these frogs were resistant. We hypothesized that chytrid infection in the Zion Canyon tree frogs were not lethal, but other environmental factors caused populations to fluctuate. Canyon tree frogs were captured and swabbed in each of nine canyons in and around Zion National Park during the summer of 2014, and total frog populations were counted in each study canyon. The swabs were analyzed for the presence of chytrid DNA, and infection rates and population sizes were compared with data from 2011-2013. We found that the presence or absence of chytrid had no influence on population size in a particular canyon; population sizes were more dependent on precipitation. The Zion canyon tree frogs resist this normally lethal disease; how they evade it will be the goal of future studies.

Aimee Newsham, Dixie State University Life Sciences Millions of people are infected yearly with resistant pathogens, including MRSA (methicillin- resistant Staphylococcus aureus), a biofilm-forming pathogen that is often transferred to patients from contaminated surfaces. Therefore, improved methods to destroy biofilm- encapsulated pathogens or to prevent their initial formation are required. This research is focused on the development of a safe treatment against biofilms by integrating organic salts, or ionic liquids (ILs), into different surfaces. Textiles were integrated with ILs to prevent formation of biofilms/bacterial growth, and were also treated post-exposure to determine if the biofilms could be destroyed post-contamination. Effectiveness of newly designed ILs were tested via inhibition zone studies on LB agar plates, and post-treatment samples were analyzed via scanning electron microscopy for presence of bacteria. The bacteria tested included Pseudomonas aeruginosa, Staphylococcus epidermidis, and Escherichia coli. These microbes are similar to MRSA in that they form biofilms comprised of extracellular proteins, DNA and polysaccharides. Bacterial colonies encapsulate themselves with biofilms to provide protection from threats, including antibacterial drugs. By integrating ionic liquids into textiles, formation can be prevented by IL solvation and sequestering of the extracellular biofilm components, including the proteins and DNA. This research could have tremendous implications regarding defeating bacteria that are resistant to existing treatments due to biofilm encapsulation. Additionally, the results could lead to new antimicrobial textiles and new approaches to prevent adherence and growth resistant biofilm-encapsulated pathogens.

Jeff Mecham, Brigham Young University Life Sciences Breast cancer is diagnosed in one of every eight American women. But, a safe, effective treatment for cancer has yet to be developed. Resveratrol, a naturally occurring phenol found in the skin of grapes, shows promise to be a powerful but safe chemotherapeutic in a sea of otherwise damaging and toxic treatments. However, the mechanisms by which resveratrol operates are yet to be fully understood. Our project focuses on the mechanisms by which resveratrol induces cell death in breast cancer cells. We will focus on the mechanisms of the p53 pathway. The protein p53 operates as an internal housekeeper of the cell. When DNA is damaged, p53 assesses the damage and can cause cell death when needed to prevent the spread and replication of the damaged cells. Cancer cells often decrease the amount of p53, allowing them to replicate without hindrance. Cancer cells treated with resveratrol show an increase of the amount of intracellular p53 restoring the cells’ ability to induce cell death. Our project focuses on two apparent mechanisms by which this increase occurs: calcium signaling and the decrease of ubiquitination of P53. Ubiquitin is a protein used to mark other proteins for degradation. Other papers and studies have shown that resveratrol inhibits key players in the process of ubiquitinizing p53. We will focus on resveratrol’s effect on both the ubiquitinizing and deubiquitinizing machinery, including G3BP1, USP10, and MDM2. A better understanding of the mechanisms by which resveratrol leads to the targeted death of cancer cells is an important step towards better cancer treatments.

Jordan Tingey, Brigham Young University Life Sciences Diabetes is a serious condition that is increasing worldwide. Diabetes is characterized by lost β-cell mass and uncontrolled blood glucose levels. Pancreatic islet transplantation could be used to cure people with diabetes, however the lack of islets is a major obstacle to its use. If we could understand how to increase β-cell proliferation and glucose stimulated insulin secretion (GSIS) then we could increase success in pancreatic islet transplants. Nkx6.1 induces β-cell proliferation. Nkx6.1 mediated proliferation is dependent on expression of Nr4a1 and Nr4a3. Nr4a1 and Nr4a3 are orphan nuclear receptors. It is currently unknown what ligand induces their activation. Previous reports have shown that free fatty acids induces expression of Nr4a1 and Nr4a3 in muscle, liver and adipose tissue. We show that culture of our INS-1 832/3 β -cell line in the presence of 0.2 mM palmitate induces expression of Nr4a nuclear receptors. Furthermore, culture with 0.2 mM palmitate results in increased β -cell proliferation. Finally, using INS-1 cells cultured with palmitate, and INS-1 cells deficient for either Nr4a1 or Nr4a3 we demonstrate the effect on mitochondrial respiration. Our data demonstrate that free fatty acids that are present during diabetes may induces expression and enhance activation of the Nr4a nuclear receptors, thus resulting in enhanced β -cell proliferation.

Alex Bischoff, Connor Dodge, Sarah Livingston, Sterling Rosqvist, Tomonori Baba, Kyle Larsen,

Alysa Edwards, University of Utah Life Sciences Percutaneous devices (PDs) constitute foreign materials that penetrate through the protective skin barrier to provide connection between internal and external environments. It has been previously shown that the periprosthetic tissue at the PD-skin interface is under a continuous state of wound healing, which often results in epidermal downgrowth. This continuous downgrowth is detrimental to the long-term survival of these devices. To date, there are no effective methodologies available to either prevent or quantify the degree of epidermal downgrowth indicating a need to find effective markers to document the healing response around these devices. In this study, periprosthetic tissues from a previous pig- back study were subjected to two different evaluations: (1) standard histology (HandE) and (2) immunohistochemical staining (IHC). Healing responses around PDs made with different material types were examined using cytokeratin 6 and collagen 4 to determine the degree of wound healing and granulation tissue maturity. Varying exposure time and concentration of stains, staining procedures were optimized. The interfacial tissues were then analyzed using either a photo or a confocal microscope. Preliminary data (Figure 1) indicated that there were noticeable differences in the periprosthetic regions between the material types used. The IHC data confirmed that the periprosthetic tissue is a hyper cellular region with a high density of blood vessels (collagen 4) and migrating keratinocytes. This data further confirmed the morphological differences observed between implant types using standard histology. Continued analysis will quantify the amount of collagen 4 within the periprosthetic tissue using imageJ software. Semi-quantitative data from each implant type will then be compared to predict biocompatibility. This research has demonstrated that IHC staining could be a potential tool for understanding the healing cascades around the percutaneous device.

Derek Munday, University of Utah Life Sciences Cardiovascular diseases (CVD) are more prevalent in individuals with diet-induced obesity (DIO) and type two diabetes (T2DM). Examples of CVD include blood vessel dysfunction and systemic hypertension. Both of these pathologies are associated with a reduced ability of the inner lining of the blood vessel (the endothelium) to release a substance (nitric oxide) that causes the blood vessel to dilate. At present the mechanism whereby T2DM and DIO decrease the function of the enzyme (nitric oxide synthase; NOS) responsible for nitric oxide synthesis and release is unknown. Determining this mechanism is the current focus of our laboratory. Earlier we reported that the sphingolipid ceramide is elevated in cell models of lipotoxicity and in mice with DIO. Most recently in endothelial cells we showed that ceramide causes protein phosphatase 2A (PP2A) to bind directly with NOS which disrupts the interactions among proteins that are necessary for optimal NOS function. My overall project was concerned with determining whether this mechanism is operational in mice with DIO. Specifically, we tested the hypothesis that PP2A inhibition would preserve vascular protein- protein interactions required for optimal NOS enzyme function to an extent that arterial dysfunction and hypertension would not occur. Mice consumed a control (CON) or high fat (HF) diet for 12 weeks. During the last 2 weeks, cohorts of mice from each group were injected (IP) with saline (vehicle control) or the PP2A inhibitor LB1 (1.0 mg/kg/day). We observed that interactions among proteins required for optimal NOS enzyme function were disrupted in arteries from mice with DIO treated with saline but not with LB1. Furthermore, arterial dysfunction and hypertension existed in mice with DIO that received saline but not LB1. These results strongly suggest that PP2A activation contributes importantly to arterial dysfunction that exists in a pre-clinical model of DIO.

Scott Frodsham, Brigham Young University Life Sciences The goal of this study is to better understand if the genetic variants that strongly correlate with an increased risk of developing multiple sclerosis (MS) also increase the risk of developing diseases that commonly co-occur with MS. This relationship can be determined by comparing genetic data of patients diagnosed exclusively with MS to the genetic data of patients diagnosed with both MS and one of its comorbid diseases. Many electronic medical records (EMR) collected at medical institutions are made available for research purposes. The EMRs of individuals contained in the database that will be used for this study are linked to corresponding genetic information. Data extraction via computer algorithm will be executed to identify patients who, because of their respective diagnoses, will provide meaningful data for analysis. The case group for individuals diagnosed with just MS and have available genetic information consists of 1003 individuals. Applying a basic algorithm (ICD-9 billing codes) to this group has shown preliminary data on patients with MS and one other comorbidity as follows: Hypertension, 192 patients; anxiety, 17 patients; hypothyroidism, 84 patients; Type 1 diabetes, 24 patients; inflammatory bowel disease, 12 patients; migraine, 116 patients; restless leg syndrome, 14 patients; rheumatoid arthritis, 28 patients. The algorithms will be modified to find and include more patients for analysis. We will enhance patient identification by including medications and text keyword searches of clinical notes in the search. Genetic analysis will be performed on the final dataset.

Brooke Linney, Brigham Young University Life Sciences Recombinant DNA technology has become one of the most critical fields of research relating to biotechnology. Recombinant DNA can be used to obtain certain proteins or examine the effects of genes that we engineer, with many applications in medical research. As part of our lab’s use for recombinant DNA, we create a gene sequence to code for a certain protein, and then use heat-shocking transformation to stimulate Escherichia coli bacterial cells to incorporate the mutated DNA from the surrounding solution. As the bacterial cells then grow, they replicate the mutated plasmid that we introduced. This DNA can later be extracted from the bacterial cells and used for further synthesis, usually protein synthesis in our lab. The process of transforming bacterial cells with mutated DNA is directly affected by plasmid size. Transformation efficiency is maximized with smaller plasmids. One of the DNA plasmids we use to introduce mutations is the pET9a vector. This plasmid is a sequence of 4,341 base pairs, but by reducing the length of the plasmid, we can increase transformation efficiency. By restricting the size of the pET9a vector, we will also be able to introduce larger foreign DNA sequences than we would with the original pET9a vector. This presentation will explore the different methods of reducing sequence length to optimize the pET9a vector, mainly focusing on site-directed mutagenesis coupled with the use of restriction enzymes.

Christian Kindt, Alyssa Ashton, Jay Homewood, and Catie Nielson, Brigham Young University Social and Behavioral Sciences Background: Improved understanding of the neural mechanisms of anxiety in ASD may provide targets for etiological research as well as for better treatment specificity. To date, however, there have been no fMRI studies of such mechanisms. Objectives: We report findings from behavioral and functional neuroimaging studies of potentially atypical function. We hypothesize that such atypical function during extinction of learned fear may contribute to difficulty adapting to changing contexts in ASD and lead to subsequent symptoms of uncertainty and anxiety. Methods: Twenty-one adults with ASD ages 18-29 were compared to healthy controls on a classical conditioning task used by Phelps and colleagues (Hartley et al., 2011) during two functional runs of fear acquisition and two extinction runs. Resulting regions of interest were identified with a 2×2 repeated measures ANOVA comparing diagnostic group (ASD vs. controls) and task condition (threat vs. non-threat stimulus). Results: Analyses revealed greater activation in right amygdala and left insula in controls than ASD for threat compared with non-threat stimuli. ROI analysis across all 4 runs revealed that activation patterns for these ROIs show a statistically significant descrease for the CON group, in contrast to an increase for the ASD group that persists into the extinction period. Left amygdala and right insula showed significantly greater activity in the ASD group compared to controls in final extinction run. Conclusions: fMRI data support that fear learning networks in amygdala and insula are less activated in ASD during fear acquisition, but show increased activation during contexts that should be safe. Rather, they are afraid when they should be feeling safe. We hypothesize that chronic everyday anxiety in many individuals diagnosed with ASD may arise from uncertainty regarding contextual cues for fear versus safety.

Crystal Garcia, Corbin Standley, Kaitlin Staker and Lyndsi Drysdale, Weber State University Social and Behavioral Sciences The extant research suggests that there are relationships between differing personality types and Internet and Facebook usage (e.g., Buffardi and Campbell, 2008; Ross, et al., 2009). To that end, the present study aims to determine which personality traits, including narcissistic traits, predict Internet and Facebook usage. In addition, it examines specific motivations for continued and consistent use of the social media site, including the types of activities one engages in while using Facebook. Participants completed a survey measuring narcissism, the Big 5 personality dimensions, Facebook usage, specific activities (e.g., unfriending, blocking others), and motivations (including emotional motivations) for using Facebook. It is predicted that different personality types will differ in the amount of time spent on Facebook. Moreover, motivational reasons as to why participants first joined Facebook, and why they continue to use Facebook will also differ in relation to personality dimensions. More specifically, we anticipate that those scoring higher on the narcissism scale will report higher Facebook usage. Furthermore, we anticipate that those scoring higher on the narcissism scale will be more motivated to use Facebook for self-esteem and personal-related reasons as opposed to professional or more social reasons. The results of this study will lead to a better understanding of the factors that contribute to the need and compulsion to actively interact, know, and inform others of what one is currently doing, feeling, and thinking through social media sites, specifically Facebook.

Ho Yan Mok, Dixie State University Social and Behavioral Sciences Game research is troubled with utilizing a number of different concepts used to forecast the often negative outcomes of playing games (Utz, Jonas, and Tonkens, 2012). These concepts often extend over each other and frequently put too much emphasis either on negative consequences or game-specific aspects that makes it lack comprehensiveness. This paper will argue that the field of gaming addiction can benefit from using concepts that are already well-established in alternative domains and extending earlier work to also cover the different benefits of gaming. Based on previous research on gaming, the dualistic model of passion is recommended in the current study to give a better understanding of antecedents and positive outcomes of gaming. This model proposes that gaming promotes flow experience, which consequently results to higher level of achievement in school, less anxiety, and higher self-esteem, to mention a few (Sherry, 2004).

Holly Clegg and Charles Yeager, Snow College Social and Behavioral Sciences Economic growth can be defined as an increase in the amount of goods and services produced per capita over a period of time. Basically, economic growth, from a geographic perspective, can be summed up as what types of businesses grow where – and why. Logically, improvements in quality of life [QOL] accompany economic growth. This research focuses on three measures that represent QOL: poverty, education, and access to health insurance, and measures them as they relate to economic growth at the county level in Utah. Data was mapped using a geographic information system, and correlations between economic growth and QOL variables were determined. The purpose of this research is to determine if economic growth necessarily produces measurable increases in QOL variables, or if economic growth tends to occur in areas where QOL is already higher.

Corbin Standley, Weber State University Social and Behavioral Sciences The extant research regarding the relationship between religious affiliation and attitudes toward homosexuality is extensive, yet shallow. While some research suggests a significant positive correlation between religiosity and negative attitudes toward homosexuality (Ingelhart, 2000), other studies show different results dependent on religious motivations, affiliations, and activity (Besen and Zicklin, 2007; Burris, 1999). To that end, the current study examines the interplay between religiosity, personality dimensions, and attitudes toward homosexuality. A correlational survey methodology was utilized attempting to measure religious activity and motivations, personality dimensions, and attitudes toward various policy areas as relevant to homosexuality (including marriage, adoption, and military service). A significant positive correlation between religious activity and negative attitudes toward homosexuality is anticipated such that those scoring higher on the religious activity scale will report more conservative views of homosexuality. Moreover, we expect a strong positive correlation between the Openness to Experience personality dimension and positive attitudes toward homosexuality such that those expressing more openness will exhibit more liberal views toward homosexuality. The results of this study will lead to a better understanding of the ways in which religious affiliation and religious activity differ in regard to attitudes toward homosexuality. Further, implications concerning personality traits in relation to such attitudes will be drawn from these results.

Sarah Axtell, Brigham Young University Social and Behavioral Sciences The term “skin whitening” implies that one’s skin is whitened through engaging in some type of action. In Lawson’s Bay Colony, a northern suburb of Visakhapatnam, India, many women are engaged in skin whitening. While skin whitening is most commonly thought of as applying lightening creams, women say that skin whitening can also be done through actions like eating certain foods, staying indoors, or even just relaxing. Despite the large amount of women engaged in skin whitening, many of them say that skin whitening does not work. Through ethnographic research in Lawson’s Bay Colony, it became clear how skin whitening in a broad sense actually has nothing to do with the skin at all, so reports on the effectiveness of the creams or other skin whitening measures are not relevant. Rather, in the Indian context, it does not matter what happens to the skin through skin whitening because of the intricate connection between the soul and the body. So, when women say that skin whitening does not work, they are referring to how their body color does not permanently become lighter, but this does not mean that their soul/body is unaffected. Therefore, when a woman’s soul is lightened—or if it is already culturally white—her body is as well, so instead of skin whitening, women are engaging in body/soul or holistic whitening.

Catherine Cragun, Brigham Young University Social and Behavioral Sciences Sibling relationships play a critical role in healthy development throughout childhood, adolescence and into adulthood (Dunn, 1983; Jensen, Whiteman, Fingerman, and Birditt, 2013; Stocker, Lanthier, and Furman, 1997). The quality of the relationship matters as well: a predominantly warm relationship is linked to less antisocial behavior, yet if there is mostly conflict it can be linked to depression and anxiety (Padilla-Walker, Harper, and Jensen, 2010). While the influence of the sibling relationship is well documented, much less is known about what influences it. Marital conflict is one likely deterrent to positive sibling relationships (Stocker and Youngblade, 1999), but there has been little attention to the effects of marital conflict and intimacy over time. Our study will analyze how changes in marital conflict impact future warmth and conflict between siblings. Data for this study come from the Family Relationships Project (FRP). The FRP surveyed two parents and two children from 200 families 13 times (phases) from 1995 to 2012. During in-home and web-based interviews parents and children reported on their family relationships and personal development. Using SAS we are conducting a series of lagged multi-level models to examine how the marital relationship at an earlier phase relates to sibling relationships of the next phase. We will then juxtapose our analyses from the first seven phases with the final six to observe any long-term correlations. Preliminary analyses have revealed a negative correlation between marital conflict and siblings’ levels of intimacy during childhood (r = -.17, p < .05), but not in adolescence (r = -.10, p < .05). In conclusion, we anticipate that our complete analysis will support the hypothesis that marital relationships play a role in the development of sibling relationships but that those links vary across the developmental periods of childhood, adolescence, and early adulthood.

Catie Nielson, Alyssa Ashton, Alexis Grow, Christian Kindt, and Jay Homewood< Brigham Young University Social and Behavioral Sciences Studies have found co-morbidity of ASD and social anxiety disorder, but it is unclear how the anxiety experienced in ASD is socially mediated. In this study, we measured psychophysiological reactivity during socially stressful (performance evaluation) trials compared to unevaluated trials. We aimed to understand how anxiety in people with ASD is mediated by fear of negative social evaluation. We hypothesized that the ASD group would show elevated stress to both types of threat while the control group (CON) would be more affected by social evaluation than non-social contexts. Twenty adults aged 18-29 diagnosed with ASD were compared to age- and IQ-matched controls on modified Stroop and Multi- Sensory Integration tasks. We measured stress with impedance cardiography and skin conductance response. In a computerized task, participants were instructed for each block whether or not the research assistant and computer would evaluate them. We examined within subjects differences for evaluated and unevaluated trials, as well as between subjects with ASD and CON groups. We found that adults with ASD had higher physiological responses, relative to controls, during stress conditions. Parasympathetic activity during recovery periods was reduced in the ASD group. There were significant group X evaluation condition interactions, with the evaluated trials adding substantially more to the stress response in the CON but not the already elevated ASD group. Response to social evaluation was significantly correlated with scores on the Fear of Negative Evaluation and the Social Anxiety Questionnaire in both groups. Increased sympathetic activity during stress and decreased parasympathetic activity during rest confirm other recent studies that show ASD adults are out-of-sync with fear versus safety contexts, which may underlie their everyday anxiety. Interventions for anxiety in ASD should focus on helping individuals recognize physiological stress responses and develop situation-specific coping skills.

Kirsi White, Tracy Clemans, and Craig Bryan, University of Utah Social and Behavioral Sciences Sexual exploitation is correlated with high rates of posttraumatic stress disorder. PTSD is a well-established risk factor in suicidal thoughts and behaviors, but to date there are few studies examining these issues among survivors of commercial sexual exploitation. The purpose of the current study is to assess the relationships among suicidal ideation and suicide attempts with trauma among adolescent female survivors of commercial sexual exploitation in Cambodia. We plan to assess the prevalence of suicidal ideation within a sample of commercially exploited children. Data are being collected as a part of a pilot study evaluating the feasibility of cognitive processing therapy (CPT) for the treatment of PTSD among 13 adolescent female survivors of commercial sexual exploitation in Cambodia. We will collect data from baseline interviews with 13 female adolescent participants with ages ranging from 14 to 19 years old, using the Self-Injurious Thoughts and Behaviors Interview (SITBI; Nock et al., 2007). To see if there is change in suicidal ideation from the baseline assessment to the completion of the study, we will look at the 13 participants’ responses to SITBI items at the 1 week follow up and the 3 month follow up assessments. The results in the current study will provide information on rates of suicidal thoughts and behaviors in a unique sample of adolescents, and will provide preliminary information about the effectiveness of cognitive processing therapy (CPT) for suicidal thoughts and behaviors in this population.

Jamie Slade, Utah Valley University Health Electronic cigarettes (e-cigarettes) are gaining in popularity. Unfortunately, this increase is occurring at a time when we lack a definitive understanding of the health hazards. It is important for professionals to understand e-cigarette users’ experiences and satisfaction with the devices in order to determine what may entice users to begin and continue using these devices.

Daisha L. Cummins, Kodey Meyers, and Breanna E. Studenka, Utah State University Health Children with Autism Spectrum Disorder (ASD) exhibit rigidity of motor plans and difficulties planning and executing movements (Eigsti et al., 2013). Those with ASD may also have difficulty formulating new or switching between different motor plans. In typically developing individuals, sequential actions exhibit hysteresis, a phenomenon where a specific motor plan is influenced by recent, similar motor actions. We sought to determine if hysteresis was stronger in children with ASD. A rotation motor task measured the rigidity of motor planning (hysteresis) of five ASD children, and 5 control participants. A stick was placed in one of 24 different orientations around a circle. The researcher moved the stick counterclockwise or clockwise in subsequent trials. A participant grasped the stick and returned it to the home position. Researchers measured the position at which the child switched from a thumb up to a thumb down grasp in each direction. The peak counterclockwise switch occurred later for children with ASD. The grasp also changed less frequently for the ASD than for the control group. Our results suggest that changing a grasp was more costly than being comfortable, and that hysteresis was more prevalent in children with ASD than in the control group.

Abigail Harris, Brigham Young University Health Purpose:

Tianne Pierce, Utah Valley University Health Since Mario and Zelda (Nintendo video games), video interactive games have been a favorite babysitter for the past two generations of children, simulation is no stranger to this population. Portions of this population became nursing students. Simulation in health care is second to none in the ‘hands on’ teaching of skills; thus, it would be the natural order of things to include interactive figures and scenario during teaching and learning in nursing. Utah Valley University’s nursing department employs the use of human simulators in the delivery of content to the students. It is no surprise that these students relate well to simulation in the classrooms. The purpose of the research study will be to compare students’ responses to learning in a teaching environment void of simulation vs. a teaching environment which uses simulation. Although simulation has long been used in aviation and the military, it has become more integrated in the health care profession over the last 20 years. These study results were congruent with national and international landmark studies where the use of simulation in nursing has been supported by the world of healthcare.

Diana Carter, Brigham Young University Health Purpose:

Ashley Havlicak and Joanna Beachy, University of Utah Health Background:

John Rossi, Julie Valentine, Leslie Miles, Linda Maybe, and Julie Melini, Brigham Young University Health The purpose of this study is to explore the effects of sexual assault on memory and consciousness in 314 victims in a Mountain West community using retrospective chart review. Altered mental awareness and/or loss of consciousness during a sexual assault are widely unexplored phenomena. A majority of assaulted individuals experience a varying degree of loss of consciousness during an assault – resulting in memory loss and conflicting evidentiary reports creating challenges during an investigation and prosecution. Statements written by a forensic nurse based on patients’ telling of the sexual assault (analyzed in Nvivo10) were placed within the following categories: memory loss; decreased feelings of mental alertness or awareness; symptoms of tonic immobility; detachment from self, environments and/or situation; reports full loss of consciousness; and/or awoke to assault. Quantitative data from patients’ answers to questions related to their memory of the nature of the assault was analyzed through descriptive statistics in SPSS, resulting in the following statistics: 58.3% reported having a loss of consciousness or awareness, 54.2% reported “unknown” to 1 or more questions about the nature of the assault, 37.8% reported “unknown” to 4 or more questions, 17.3% reported “unknown” to all questions. Additionally, a Chi square test for independence found patients with memory loss/altered consciousness were associated with the following variables: patients with mental illness and/ or use of psychotropic medications (p=0.025) and use of alcohol prior to assault (p=0.000). Medical teams, law enforcement, and judicial representatives must understand the impediments associated with a victim suffering from altered mental status caused by neurobiological and psychophysical effects of sexual trauma; thus, creating an atmosphere that avoids re- traumatizing a patient and providing for optimal care. This research will supplement other studies focusing on neurobiology/sexual assault trauma and foster greater understanding of the effects of sexual assault on memory and consciousness.

Kalene Mears, Brigham Young University Health The purpose of this project is to compile a learning history for a hospital unit, documenting patterns of past adaptation within a clinical microsystem that can be magnified to help the system continue to improve. The goal in healthcare is to create an adaptable system, where changes are continuously made as problems are identified to prevent harm from care. Through a learning history, a hospital unit can identify how they have already solved problems in the past and identify patterns of adaptation that can be replicated to increase reliability within the organization. This project has the potential to help a hospital unit improve its reliability in providing quality patient care and reduce preventable patient harm. To obtain the information about the unit’s history, key team members will be interviewed using an appreciative approach to identify strengths and patterns of successful adaptation. Interviews will highlight positive features of this team to foster feelings of success and achievement, promoting desire to continue improvement. Clinical data will supplement the interviews to show a full picture of how the unit adapted to its situation. The transcribed interviews will then be distilled to highlight the critical, meaningful points in the plot line where learning and adaptation took place. This resulting manuscript is the tangible learning history. Internal dissemination will involve sharing the learning history with the organization and facilitating group discussions about the findings with members of the organization. Through this process, we anticipate the unit will be able to promote more effective interventions to improve patient care.

Trey Esplin, Alathia Burnside, Sean Madill, Marquelle Funk, Sean Kiesel, Kaisey Margetts,

Cody Craig and Charles Yeager, Snow College Health Logically, a rise in income should lead to a healthier population. Diseases like malaria, cholera, and HIV/AIDS are associated with poverty, low rates of education, and poor infrastructure in many parts of the world. In populations with higher per capita income (or “wealth”), these diseases are not as common. However, while our higher socioeconomic classes have fewer poverty linked diseases, is the overall health of people across Utah as high as we might expect it to be? Diseases like obesity, heart disease, and diabetes (known as diseases of affluence) are becoming more and more common, even though advanced health technology and healthcare availability increase. This project will examine the relationship between income level, diseases of poverty, and diseases of affluence. Data will be aggregated from a number of sources and mapped using a geographic information system. A regression analysis will be conducted to determine if variables are positively or negatively correlated with diseases of poverty and affluence in Utah.

Whittni O’Brien, Dixie State University Health St. George residents are currently at risk from poor indoor air quality. The objective of this study was to provide citizens with simple solutions to purify their indoor air and avoid the side effects of pollution. The rising threats include benzene, formaldehyde and trichloroethylene. These carcinogens are directly linked to heart disease, birth defects, asthma and premature deaths in individuals. The study was conducted to see just what could be done to combat these hazards and provide the necessary information to resolve the levels of indoor air pollution to residents. A standardized questionnaire was issued to find out how educated locals were about the pollution levels and air quality within the community. Questions covered a variety of aspects including exactly what the threats were to specific methods of indoor air purification. To reduce the number of carcinogenic related health issues, participants were provided with a pamphlet including easy to follow steps to cleaner indoor air and outdoor air pollution. The objective result yielded plants to be the best solution. Aloe vera plants are not only grown locally, but are easy to propagate. These plants are known for their ability to remove formaldehyde from the air and therefore an inexpensive and readily available resource that will assist residents. Other solutions found included greenery such as moth orchids, snake plants and the ficus tree. All of these plants are easy to maintain with local climate conditions. By providing residents with the proper education and resources, the overall health of the population will rise. The conclusion of the study offers vital knowledge to the community and a progressive approach to cleaner indoor air for a healthier living space.

Rebekah Goodrich, Leenalitha Panneerseelan Bharath, Ting Ruan, Tetyana Forostyan, Ashot Sargsyan,

Erika Brown and Stacie Hunsaker, Brigham Young University Health Health care professionals are often challenged with starting an intravenous (IV) line in patients who are dehydrated, have suffered trauma, or are in shock. Nurses and physicians can become frustrated by the multiple attempts and patients can lose valuable time. An alternative route to deliver the needed fluids and medications to these critical patients can be achieved by accessing the blood supply inside the bone. Intraosseous (IO) access is a safe, rapid, and an effective alternative method to deliver medications and fluids to these critical patients (Hunsaker and Hillis, 2013). I was mentored in the practice of qualitative research and interview process in a study related to difficult IV access. A qualitative research study was performed at Hospital Luis Vernaza in Guayaquil, Ecuador, to determine the complications faced in providing timely care to critical patients, and to assess their knowledge and use of IO therapy. Interviews were performed, and, because of my fluency in the Spanish language, I was the primary interviewer. These interviews were analyzed to assess the need of interventional IO therapy. Through the interviews, preliminary results demonstrated a need for an organized IV algorithm in this large hospital. The difficult IV algorithm has been developed and will be presented to the health care professionals at Hospital Luis Vernaza in the spring of 2015 on a Brigham Young University College of Nursing Global Health stay in Guayaquil, Ecuador.

Delena Hanson, Dixie State University Health Debate in diagnostic imaging over the effectiveness of shielding the patient from the incident (primary) beam compared to shielding from the scattering beam once it has hit the image receptor is ongoing. Because radiation in any amount can cause long term effects, it is the ethical obligation of those in the profession to keep the dose of radiation to the patient as low as reasonably achievable. Backscatter is radiation that goes through the patient, contributes to the diagnostic image, then still has enough energy to hit the image receptor and scatter back toward the patient. While previous studies indicate that dose to the gonads from the primary beam during chest x-ray exams are low, this additional research assesses the amount of backscatter that happens during a chest x-ray to determine at what point patient size is a factor that increases patient dose. As more technique must be used for larger patients, increased interactions will occur and therefore present a higher probability of backscatter that can add to the patient’s gonadal dose. This research quantifies whether and at what point placing a lead shield between the patient and the image receptor will reduce dose to the patient by measuring the thickness of a patient receiving chest x-ray and using a pocket dosimeter to measure the amount of radiation scattering back from the patient to compare with data from the incident beam.

Paydon Newman and Joseph Rebman, Dixie State University Health Several studies (Shepley, 2012; Sherman-Bien, 2011; Walch, 2010) have found that sunlight has a stress-reducing effect on those who are exposed to it. An innovative new design of artificial lighting known as RaLight is proposed to reflect light with a color rendering index nearly identical to natural sunlight. This study will examine the relationship between exposure to RaLight (as a substitute for sunlight) and levels of stress. RaLight is predicted to decrease levels of stress in test subjects. In a commercial call center setting, test subjects will consist of an estimated 50 employees both male and female with ages ranging from 18 to 30 years. This research is designed as a single subject experiment (ABA). The initial control condition will be the common indoor environment which exposes patients to standard fluorescent lighting. Replacing light fixtures with RaLight will then establish both the second phase of the experiment and the independent variable. A follow up re-installment of the initial fluorescent lighting will be conducted post-RaLight phase. Throughout each phase, questionnaires regarding the overall well-being of employees will be completed.

Samantha Falde, University of Utah Humanities The purpose of this paper is to closely examine the legacy of the policies and actions taken by the Soviet Union during the Cold War in order to determine its contributions to current levels of small arms and light weapons (SALW) proliferation around the globe. This examination confirms as reality the perception of the Soviet Union as the primary propagator of indiscriminate small arms proliferation in the post-Cold War era. As such, the Soviet Union was a chief contributor to the current situation of global insecurity perpetuated by the creation of extensively armed and violent societies known commonly as Kalashnikov Cultures. In examining the impact of Soviet policies on SALW proliferation, this paper utilizes the concept of the ‘‘Non-State Threshold’’ at which, when intact, small arms and light weapons are effectively segregated between legitimate state and illegitimate non-state actors, and when breached, indiscriminate spread occurs. The Non- State Threshold will be applied to the years during and immediately following the Cold War to determine under which conditions indiscriminate SALW proliferation occurred, and to facilitate a clearer understanding of how Soviet policies and actions allowed for the permeation of the Threshold by increasing the availability, ease of acquisition, and appeal of SALW to non-state actors and illegitimate groups. This paper demonstrates how the legacy of Soviet policies has facilitated the creation of dangerously armed, rogue societies, supporting the claim that it is the actions of the Soviet Union specifically that have disproportionately contributed to the creation of Kalashnikov Cultures.

Daniel Cox, Dixie State University Humanities Community correctional professionals stand between offenders and their potential relapse into criminal behavior, i.e., recidivism. These officers are expected to monitor conduct and use evidence-based practices for rehabilitation. However, this important dual-role is too often distorted. Researchers have noted that training for officers about the use of evidence- based practices filters through officers’ attributions (their values and professional orientation), thereby impeding implementation of these practices (Whetzel et al. 2011). In this presentation, I argue that training of this sort often fails apparently because its delivery does not address officers’ attributions, especially regarding stereotypes of psychology and organizational support. I plan to suggest that recidivism, as well, will drop when training accounts for how and to what extent attribution biases interact with efforts to implement evidence-based practices. These preliminary findings are drawn from a study I am leading that assesses 50 officers throughout the State of Utah, utilizing surveys provided by researchers Mario Paparozzi (University of North Carolina– Pembroke) and Jennifer Skeem (University of California– Berkeley), about their attributions prior to and after training to measure for positive change in using evidence-based practices. The study also evaluates officers’ use of evidence-based practices during interactions with offenders with evaluation tools provided by researchers Peter Raynor (Swansea University) and Faye Taxman (George Mason University). The intent of the study, ultimately, is to determine if and to what extent attribution-based training for evidence-based practices will cause positive organizational change, the outcome of which is likely not only to influence the manner in which correctional professionals are trained but also the manner in which their training manuals are written.

Latrisa Garcia, Dixie State University Humanities Statistics show that in 2013, the US population was 313.9 million people, while the number of active cell phone subscriptions was 345.2 million, which translates to 110% of the US population having active cell phones. As more people connect to the world through cell phones they become less aware of the physical world which surrounds them, known as Inattentional Blindness. This paper asks the question: Does the increased use of cell phones impair college students’ perceptions about the actual amounts of crime that occurs on campus, and if so are students under or over estimating the amounts of crime? It is hypothesized that the majority of students will under estimate the amount of crime that occurs on a college campus because of their reduced awareness of the real world. This study uses a convenience sample acquired through a web based survey conducted on a college campus of approximately 10,000 students in the southwest region of the United States. Study results will be finalized in the coming weeks. Early results suggest that cell phone use and inattentional blindness may not be as prevalent on campus as originally hypothesized.

Miranda Roland, Southern Utah University Humanities Voluntourism (volunteer tourism) is the action of using personal vacation time to assist in volunteer service usually in foreign countries. Voluntourism has recently received negative connotation as research has deemed them “non-beneficial” or even “harmful” due to the short duration spent and overall adverse effect caused unintentionally by the project. This research was conducted to determine what participants of the temporary medical clinics hosted by the Global Medical Training program really think of the provided service. This information can deem if the alternative breaks taken by pre-medical and medical volunteers are beneficial to the areas they serve and if future breaks should be considered, if not strongly encouraged. The surveys were completed verbally to the patient with the aid of a Spanish translator. Surveys contained questions that addressed the influence of how they receive medical attention due to these clinics and satisfaction of attention received during the clinic. All of the surveys (100%) signified that these Global Medical Training clinics do influence how they receive medical care with additional comments of “beneficial to the low class economy”, “traveling to us is very helpful”, and “appreciate the advice given”. 98.08% signified that they are satisfied with the medical attention received through the clinics. As demonstrated through these results many communities are satisfied with the service provided and they wish to see more clinics of this set-up as it relieves the cost financially and time-wise of traveling to the nearby medical center. Upon conclusion, the trips taken by pre-medical and medical students through the Global Medical Training program are deemed beneficial to both the volunteers and the recipients of their service. These trips should not be demoted by the negative connotation of voluntourism but instead should be strongly encouraged if a volunteer desires.

Rachel Sharich, Dixie State University Humanities Non-traditional students face innumerable challenges during the course of their studies but can find a successful balance if they have the proper tools and a determined mindset. Going back to school after a decade or more is a daunting task when considering work and bills, kids and dinner, laundry and car repairs. In 2007, I attempted to describe my city-girlturned- agrarian-survivalist efforts in self-reliance after gutting my extra rooster and cooking him for dinner. Then, my life changed. I became a full-time college student and after a few semesters, I realized that my knowledge of grammar and punctuation had grown significantly since my rooster-killing musings. I re- learned comma rules, proper use of semi-colons, and the difference between a dash and a hyphen. Although storytelling is a personal strong suit, I now see many flagrant errors in my past writing. Comma splices are scattered throughout email, journal entries, and even handwritten notes. I overburdened any sense of idiosyncratic expression with abundant stylistic fragments. I attach my success or failure as a human being to the letters on my report card; I have always been an ‘A’ student. College cannot be the top priority for most non-traditional students. I strive for some sense of lop-sided equilibrium each day and encourage other potential students to realize their own dream of earning a college degree, no matter their circumstances. I enjoy assisting other students in correcting their own writing errors. I am content with any passing grade because I am seeing my work, and my life, change for the better.

Jordan Kerns, Dixie State University Humanities In my creative compilation, I explore four different classifications of the word blood in four flash non-fiction pieces. I utilize the writing technique of compression, so none of my stories exceed 400 words. Flash pieces give information to the reader through the techniques of inference and understatement. Throughout my stories, I show readers my relationships with other people, my thoughts about myself, and my opinions about certain possessions through the use of flash methods and with an underlying theme of blood. The first story, “Cremation,” illustrates my relationship with my brother through a cryptic conversation we had about death—its tie to the theme being our blood relation. The next piece, “Blood Lines,” describes a pair of sweatpants I stole from my father with subtle clues that hint at the bad blood left between him and my mother and my mother and me. The third flash, “Blood and Frosting,” is a second-person narrative about the process the narrator takes to make red velvet cupcakes for her friends and family. The last work, “Syrup and Sky,” is another descriptive paragraph about a picture I drew in high school of a poorly-drawn wolverine covered in the blood of his kill. I aim to connect with my readers through these simple moments of life, make them feel either happy or sad or anything in-between, and make them see the beauty and complexity in mundane things.

Matthew Dye, Snow College Humanities Many Native American reservations in the United States are increasingly relying on casinos and other forms of gambling to bolster local economies. However, since the state of Utah does not allow gambling on tribal lands, Native Americans in Utah must rely on other activities to bring tourism to their communities. Many resources that are available to tribes across Utah, including pow wows and other cultural events, have become central economic activities and means for increasing tourism to tribal lands. These cultural events serve two purposes, as they serve as a means of cultural education to the larger public, in addition to providing a mechanism for increasing tourism. For this project, I will focus on how the different Native American tribes in Utah use their own unique cultures to attract tourism, and its accompanying economic benefits, to their communities.