Fine Arts

Authors: Kayla Knaperek, Megan Frisby, Geoffery Zahn, Alma Laney. Mentors: Alma Laney. Insitution: Utah Valley University. In February 2021, mycelial growth was found on blueberries, leading to the obtention of a fungal isolate using potato dextrose agar (PDA) after surface sterilization of the blueberries. The rapid growth of white mycelium and sclerotia was observed on the medium. Inoculation of basil plants with spores from surface-sterilized sclerotia in potato-dextrose broth resulted in necrosis and spreading in terminal buds, ultimately leading to plant death. Further isolation of the identical fungus from the diseased basil tissue confirmed its pathogenicity. Analysis of the isolate's ITS region sequencing revealed a 90% nucleotide identity to the closest relative, Botrytis cinerea. A project was initiated to further characterize the fungus, pathogenicity, life cycle, and morphological traits. The growth and development of the fungus were observed on V8, cornmeal, blueberry, and potato dextrose agars to characterize its nutritional requirements and growth patterns. Host range testing involved the systematic inoculation of various plant species (which were used as growth media), followed by careful symptom monitoring and photographed documentation. Additionally, Koch’s Postulates will be conducted to establish the causal relationship between fungal infection and the observed disease symptoms in the host plants. Seven days after inoculation, necrosis, wilting, and leaf discoloration were observed on select leaves of Lavender var. Oregano, suggesting possible infection by the blueberry fungus. Considering potential host plant immaturity during the initial inoculation, a re-inoculation is planned to ensure successful infection and to obtain conclusive results. Further investigation is warranted to confirm the pathogenic impact of the fungus on host plants.

Authors: Hayley Karns, Dylan Bryant, Noah Christensen, David Kirby, Sarah Marchant, Sarah Nicholas, Emmaline Saunders, Hannan Thomas, Nathan Smith, Dan Cavan, Daniele Ghiretti. Mentors: Alessandro Zanazzi. Insitution: Utah Valley University. Arthropods play a crucial role within terrestrial ecosystems by virtue of their substantial consumption of plant matter, their status as primary prey for various organisms, their function as key recyclers of organic material and nutrients in ecosystems, and their pivotal role as primary pollinators. Ground beetles (Coleoptera: Carabidae, or “Carabids”) represent a prominent and highly diverse taxonomic assemblage among arthropods inhabiting temperate forest floors. Despite their significance in these ecosystems, little is known about their trophic structure and level. These knowledge gaps primarily arise from the limitations associated with traditional investigative methods. For instance, the observation of feeding behavior in carabids proves challenging due to their diminutive size and predominantly nocturnal foraging habits. These uncertainties hinder the attainment of an understanding of community structure and trophic interactions both within the arthropod realm and with the vertebrates in their ecosystem. As a result, this research aims to gain a greater knowledge of the trophic structure of carabids using 15N/14N and 13C/12C isotopic analysis. Ground beetles were collected using pitfall traps placed near Big Springs Park (Provo Canyon, northern Utah) in September 2023. The traps were made with two plastic cups: a smaller cup was suspended inside a bigger cup. The smaller cup was partly filled with vinegar to attract the insects whereas the bigger cup was partly filled with monoethylene glycol, a compound that has been shown to preserve the insects without affecting their isotopic composition. Insect samples were collected over a period of four weeks. The beetles were then cleaned with a 2:1 mixture chloroform and methanol, dried, ground in an agate mortar and pestle, and placed in tin capsules for the isotopic analyses. Once the results are obtained, mixing model analysis will allow us to estimate the proportions of different food items based on the stable isotope ratios of consumers and food items and on known fractionations. This knowledge will contribute to a greater understanding of trophic structures and carbon pipelines in local ecosystems of northern Utah, and to a better prediction of ground beetle response to ongoing global warming and the associated changes in forest environments.

Authors: Stone Smith, Ben Belt. Mentors: Sally Rocks. Insitution: Utah Valley University. Microplastics are particles less than 5 mm in length. These particles act as a vector for heavy metal and carcinogens, which have negative effects on human health. Often invisible to the naked eye, microplastics are extremely mobile and have been found in the most extreme parts of the planet, from the peak of Mount Everest to depths of the Mariana trench. Because microplastics are readily transported via air and water, microplastics have been found in every location exposed to wind or rain. However, one place that is sheltered from wind and rain are caves. In some subterranean environments, the earth acts as a natural filter of the water that descends into caves. It is suspected that caves will have lower levels of microplastics present in their environments compared to surface environments. However, another mechanism for microplastic introduction is human activity. Humans utilize plastics in relatively all synthetic items. Everything from clothing to packaging to regular household items employs the use of plastics, which can abrade to release microplastics. The goal of this research is to determine if caves with high levels of human visitation have higher concentrations of microplastics than caves with little to no human visitation. Analysis of subterranean water samples will reveal if microplastics are invading cave environments regardless of human activity, and through what mechanisms. A consequence of microplastic pollution in cave environments is the capture of plastic particles within mineral matrices. As mineral rich water evaporates in caves, the minerals left behind form speleothems that could capture plastic particles and thus preserve them (e.g. stalactites, stalagmites, soda straws, etc.). With a better understanding of the presence or absence of microplastics in caves, efforts can be made to minimize plastic accumulation and prevent microplastics from entering the geologic record of the cave.

Authors: Haylee Beck, Lauren Weidner, Carl E Hjelmen. Mentors: Carl E Hjelmen. Insitution: Utah Valley University. Blow flies, scientifically known as family Calliphoridae, are important primary colonizers of remains that play a crucial role in decomposition, making them useful in forensic investigations for estimating a time of colonization (TOC). Unfortunately, there is little information on the species and distributions of blow flies present in Utah. Our research seeks to address this issue through collection, preservation, and identification of these blow flies. To collect the specimens, traps baited with chicken liver were set up in various locations across Salt Lake and Utah Counties. While we have only collected from these two counties thus far, our intention is to gather flies across the state, spanning both rural and urban environments. We will then use standard metrics of diversity to compare the species makeup in each location. The results of our research offer significant insights into the biodiversity, distribution, and ecological functions of these flies and their development in different ecosystems. In addition, this research has far-reaching implications in the field of forensics. These findings will contribute significantly to our understanding of blow fly distribution and behavior, providing valuable information for forensic investigations in our region.

Authors: Cori Bailie, Arlene Majers, Katherine Judge. Mentors: Olga Kopp. Insitution: Utah Valley University. Ever since antibiotics have been in use, bacteria have been evolving to develop resistance against them. Each year, the number of people who die from previously treatable infections increases, showcasing the seriousness of this global issue. According to the CDC, facilities were overwhelmed during the 2020 pandemic, decreasing antibiotic production. By refocusing on discovering and producing new antibiotics, resistance can once again begin to be combatted. Bacteria found in soil samples were used to test for antibiotic production, using safe relatives to the ESKAPE pathogens. Starting with three master plates on Tryptic Soy Agar, 39 total bacterial colonies were studied. Colonies of interest were then PCR tested for DNA sequencing, and will be run through multiple different chemical tests for classification. Results showed that three different colonies displayed antibiotic activity. Colony 7 on master plate A produced antibiotics against Bacillus subtilis. Colonies 6 and 13 on master plate B, both produced antibiotics against Mycobacterium smegmatis, and Staphylococcus aureus. The results suggest that there are more soil bacteria to be discovered that may be able to produce antibiotics for common infections that are getting harder to treat. Further testing needs to be done to determine whether these colonies are safe for human therapy.

Authors: Barbara Jetton, Carl E Hjelmen. Mentors: Carl E Hjelmen. Insitution: Utah Valley University. This project’s emphasis is the creation of an accessible and reusable tool to be used in broader scientific inquires of evolutionary relatedness. As of January 2023, GenBank contains 2.9 billion nucleotide sequences representing 504,000 distinct species. Despite this abundance of data, comprehensive and up to date phylogenies are lacking, impeding investigation into genetic histories and trait evolution. To address this problem, I am developing an open-source pipeline to expedite the construction of these evolutionary trees. I have a specific aim of creating a phylogeny for the order Diptera (flies) in order to investigate the evolution of the chromosome numbers for over 2500 species with chromosome count data on karyotype.org. I use R code, and the packages “reutils”, “ape”, and “seqinr”, to create reusable universal scripts which pull accession numbers from NCBI GenBank for each species based on the requested gene names. A second script was built to use the curated accession numbers to pull FASTA sequence data for each gene and write a multi-FASTA file for each gene, resulting in a comprehensive dataset necessary for alignment and phylogenetic tree construction. This effort will result in updated insights on the evolutionary history of Diptera related to chromosome numbers and can be used in further research in comparative biology. Additionally, these scripts can be used to investigate and reconstruct phylogenetic information for any species group with sequence data available on GenBank.

Authors: Veronica Gunyan, Minh Nguyen. Mentors: Kevin Shurtleff. Insitution: Utah Valley University. Our team’s project green hydrogen production from plastic waste by high temperature plasma pyrolysis aims to help aid in reducing waste by taking UVU’s recycled plastic waste, decomposing it using plasma pyrolysis, and turning it into clean hydrogen and carbon in a cost-effective manner. Pyrolysis is the thermal decomposition of organic materials in an oxygen-free zone. Green hydrogen comes from renewable energy and releases no CO2 by products into the air. The purity of the hydrogen can be improved by adjusting components such as temperature in the reactor, range, the gas used, the time frame, catalysts and other operational elements. Energy prospects can be determined by considering the energy consumed subtracted by that produced to determine the net output.

Authors: Brandon Barlow, Ethan Reese, Victoria Vazquez, Abbi Stark, Monica Sanchez, Alex Wilson, Rain Evans, Josef Becker, Amy Chevrier, James Taylor. Mentors: James Taylor. Insitution: Utah Valley University. This study focuses on memory and learning observed in Madasgascar Hissing Cockroaches (MHC). Research on insects can be used to inform our understanding of behavior and cognitive processes and add to our existing knowledge about the studied species. While there is existing literature focused on memory and learning in many insects, cockroaches are often chosen as a model due to their adaptability, resilience, and availability. Little research on MHC currently exists, especially in regard to memory and learning or drug seeking behaviors. This study uses a mixed model design, looking at arm choice percentages and latency to choose, between the control and experimental groups and comparing trials for each subject. In earlier trials, MHC demonstrated behavior that indicated they had learned not to fear light, a naturally aversive stimulus for cockroaches. Building on data collected in earlier trials, we developed a new protocol to evaluate the MHC associative learning behaviors and to test drug-seeking as a motivated behavior. Subjects are individually tested in a T-shaped apparatus in which they have to choose an arm to go into or are timed out. Each arm has a specific reward, either a sucrose and ethanol solution (experimental group) or plain sucrose solution (control group) in the left arm, and plain sucrose solution (experimental group) or water (control group) in the right arm. Each subject is run 2 days (6 trials per day) in these conditions, and then 3 days with the left arm lengthened to test if the subjects will seek their preferred reward even if it requires traveling a greater distance.

Authors: Benjamin Holt. Mentors: York Young. Insitution: Utah Valley University. Our research group aims to create an optical system which generates tunable longwave infrared (LWIR) light as a tool for early detection of disease-indicating molecules in human tissue. This system begins with our newly constructed Nd:YVO4-type laser which generates between five and seven watts of continuous wave optical power in the near IR spectrum. This laser light will be used to drive the subsystem responsible for generating LWIR light: an optical parametric oscillator. In order for wavelength conversion to occur via a non-linear process, the near IR laser power serving as an optical drive must have an intensity on the order of tens of MW/cm2. To achieve such intensities the laser power must be concentrated into high intensity nanosecond length pulses by Q-switching the laser. Our method of Q-switching the Nd:YVO4 laser will be via an acousto-optic modulator in the laser cavity. My work to design the modulator’s kinematic mount, cooling system, and RF driver (as well as characterizing the performance of the modulator for use as a laser Q-switch) is described in this presentation.

Authors: Juan Palencia, Tanner Probst, Clair Yeaman. Mentors: Machiel Van Frankenhuijsen. Insitution: Utah Valley University. Abstract: The ABC conjecture is an unsolved problem in mathematics first formulated by Joseph Oesterlé and David Masser. The conjecture attempts to show an analogy between polynomials and integers. This insight arises from the Stothers-Mason Theorem (also known as Mason’s Theorem). The theorem states that the maximum degree of a polynomial is always equal to or less than the number of distinct roots minus one. The ABC conjecture attempts to bridge this analogy by defining the height as the maximum algebraic number in the sum a + b = c where a, b and c are relatively prime. Masser and Oesterle define the radical as the sum of log p where p divides abc. Mason’s theorem states that the height is less than the radical minus one. It turns out that this is not true for the integers. Thus Masser and Oesterle formulated a conjecture which may be true. Over the past decades, this inequality has been strengthened and reformulated. Shinichi Mochizuki has recently published what he claims to be a proof of the conjecture, but many mathematicians are currently still working to understand it. The aim of our research is to better understand the height and radical in the ABC conjecture. In addition, we intend to explore the possibilities of expanding the Stothers-Mason Theorem. Since the ABC conjecture is concerned with an analogy between commutative rings (integers and polynomials), we are interested in seeing whether Mason’s Theorem for polynomials also holds for non-commutative rings, such as matrix rings and the ring of quaternions. In addition, we intend to explore the connection between the factorization of polynomials and finite Abelian groups.

Authors: Samantha Curnow, Carl Hjelmen. Mentors: Carl Hjelmen. Insitution: Utah Valley University. The genome sizes of insects vary immensely; some insects have only 100 million base pairs, while others reach nearly 18 billion base pairs. Many selective pressures can have an influence on genomes, but we still lack a clear understanding of which selective pressures cause such variation in genome size even within well-studied groups. To determine the influence of environmental factors on this variation, we investigate the relationship between genome size and the average natural environment of 152 Drosophila species. The nature of the relationship between genome size and environmental factors is investigated using phylogenetic models and linear analyses such as phylogenetic generalized least squares analysis. We collected data from peer-reviewed papers and publicly available datasets, such as karyotype.org and WorldClim, to compare the genome size of each species to the temperature, altitude, humidity, etc. of their natural environments using linear phylogenetic models. The distribution of these species was also visualized using geographic and biological analysis packages in R, such as geodata, to extract environmental data of specified regions, and phytools in combination with caper to run phylogenetic comparative analyses.

Authors: Roshan Abraham, Parker Jorgenson. Mentors: Daren Nelson. Insitution: Utah Valley University. Hobble Creek is an essential and valuable stream that the City of Springville and other local entities utilize for their water supply. However, the stream is also vital to the local environment and ecosystem since it one of the main streams that the June Sucker (an endangered endemic fish) spawn in each year. Numerous environmental projects and studies have been done in the lower section of Hobble Creek due to this importance. There are many springs in the region that provide water to the stream but there is a lack of consistent monitoring of the flow throughout the stream to determine how the stream is interacting with groundwater sources. Groundwater models completed by the United States Geological Survey in 2005 for the Utah Lake Watershed suggest locations where groundwater interactions should be either discharging or recharging the local aquifer. However, more recent data has suggested that many of these water interaction maps created by the USGS model are inaccurate. Using a water budget analysis, streamflow in the creek is being measured at 10 sites throughout the Hobble Creek Watershed to determine how the flow in the creek changes as it flows towards Utah Lake. Data gathered from this and previous studies show the zones of discharge and recharge shift seasonally and are not as consistent as the USGS model suggests. Recent data does confirm that Hobble Creek is gaining flow upstream and losing flow downstream, but there are portions of the river that also may be perched (do not interact with the groundwater). Further measurements and continued site investigation will be necessary to help us better manage this vital water resource.

Authors: Feyan Hoffman, Roshan Abraham, Christine Fowles, Abi Conger, Scott Jenson. Mentors: Daren Nelson. Insitution: Utah Valley University. One of the least understood part of our local water budget is the interaction of groundwater into our lakes and streams. Groundwater models completed by the United States Geological Survey in 2005 for the Utah Lake Watershed suggest zones where groundwater interactions should be either discharging or recharging into our local streams, wetlands, and other water bodies. However, more recent data has suggested that many of these water interaction maps created by the model are inaccurate, and we need a better understanding of how groundwater interacts with these water bodies. Using a water budget analysis, groundwater and surface water interactions are measured by collecting discharge measurements using flow meters at different cross sections along the Provo River and its tributaries. A gaining or losing stream may swap depending on seasonal changes, such as a particular water year being dry or wet–a stream that is typically gaining may be recharging the aquifer during a dry year. The USGS model is a good basic representation of interactions between groundwater and surface water. Our data suggests that due to highly variable water levels of Utah Lake and local groundwater levels, some discharge and recharge zones in the Provo River subbasin are likely to change over time. In addition, some sections of the river may be perched and not interact with the groundwater system at all. In order for us to better manage these important resources, further analysis of flow behaviors in the Provo River and its tributaries need to be completed. This study is part of a larger study that includes adding two new permanent flow gauging stations to provide a long term record that will enable water managers to better decipher why the groundwater and surface interactions are not fitting the USGS model.

Authors: Fatoumata N'diaye, Kevin Shurtleff. Mentors: Kevin Shurtleff. Insitution: Utah Valley University. The use of solar energy for water purification purposes has proven to be an efficient tool with promising results which could help tackle the issue of water scarcity in developing regions of the world. The low-cost, solar water purification system built previously will be improved through increasing thermal conduction, enhancement of the water flow uniformity, strengthening of the piping system, increasing airflow inside the water purification system, and thus maximizing the clean water production rate. We used aluminum foil as the condensation surface with silicone caulk as a binding agent between the materials. We used ultra-violet resistant greenhouse plastic film to protect the purifier overall from sunlight damage. We measured the incident solar irradiance, airflow inside the purifier, temperature of the evaporation side, temperature of the condensation side, ambient temperature outside, conductivity of the dirty and clean water, and clean water quality/taste. Finally, we compared this data to previously collected data.

Authors: Euiyoung Kim, Bonnie Anderson. Mentors: Bonnie Anderson. Insitution: Utah Valley University. A thermoacoustic engine is designed to transfer heat energy into sound energy. Heat engines have a hot side (hot heat exchanger) and a cold side (cold heat exchanger) and use part of the heat flowing between them to do work. The engine in this study is shaped like a bottle with copper wire mesh heat exchangers in the neck. A heating element wrapped around the top of the neck supplies heat to the hot side and the base of the bottle is made of aluminum to keep the cold side at room temperature. When the temperature on the hot side reaches a threshold the sound wave is created. The heat goes from the mesh of the hot side into the “stack” (steel wool, in this case) and down to the cold mesh. The motion of air as it picks up and drops off heat along the stack excites the engine into one of its resonant frequencies. Different mesh configurations of the heat exchangers were tested to determine the impact on the acoustic properties of the engine. Principal Component Analysis was used to determine which parameters of the mesh affected the acoustic pressure the most. The outcomes of this research not only contribute to the emerging field of thermoacoustics but also provide valuable knowledge for the development of thermal sensors and electric energy in remote locations.

Authors: Silvi Rouskin, Matthew Allan, Dragui Salazar. Mentors: Merrill Halling. Insitution: Utah Valley University. A 3’ untranslated region (UTR) is a non-coding region between the stop codon and the 3’ end of an mRNA. 3’ UTR can regulate post-transcriptional gene expression by influencing mRNA stability, translation, and localization. They fold into complex structures that contain elements and binding sites that interact with various molecules, including proteins and microRNAs (miRNAs). Despite the recognized importance of 3’ UTRs and their structural features, the vast majority of their structures in humans remain unknown. Indeed, the structures of long RNAs in general have been difficult to solve due to their heterogeneity and to the paucity of known, ground-truth RNA structures for training and validating models. This project aims to circumvent these limitations by characterizing the structures of 3,000 to 4,000 human 3’ UTRs. The project can be simply described as follows. The cDNA is received and prepared for PCR with the needed primers. After the PCR, genetic material is transcribed into RNA where dimethyl sulfate mutational profiling with sequencing (DMS-MapSeq) will be introduced. After this, it is reverse transcribed and prepared for sequencing. The project implements thousands of primers to facilitate the comprehensive identification of genes. The vast dataset of structure profiles will be used to develop an advanced machine learning algorithm to predict first the DMS-MapSeq results and eventually the structure of an RNA solely from its sequence. The preliminary results show that it is possible to determine hundreds, even thousands of 3’ UTR structures using DMS-MapSeq and the creating an accurate image of such structure. These results also contain druggable pockets that can be used in RNA based therapeutics in a near future.

Authors: Iris Cepeda, Tabitha Weir, Jonathan Cook, Honey Ordonez. Mentors: Olga Kopp. Insitution: Utah Valley University. Solanum lycopersicum (tomatoes), are beloved herbaceous crops well known for their red bearing fruits. They are best grown in well-draining soils with access to full sun and are endemic to South America but grow all across the world where the conditions allow. Tomato fruits provide a quality source of phytochemicals and antioxidants such as lycopene, vitamin C, beta-carotene, and phenolic compounds which can substantially improve one’s healthy diet. S. lycopersicum are members of the nightshade family, which are known for their production of often toxic glycoalkaloids. The presence of these compounds usually deters herbivores by increasing their concentration as a defense response. There are, however, herbivores like Manduca sexta (tobacco hornworms) that feed on members of the nightshade family by dealkylating these glycoalkaloids. It is hypothesized that plants have the ability to modulate their defensive response based on queues from the environment and from their herbivore pests. Our research objective is to determine whether a S. lycopersicum plant undergoing defoliation from M. sexta will have the same molecular response as a plant undergoing simulated herbivory. Four treatment groups will be used with each treatment group having 8 replicates. Before treatment begins, the groups will be isolated by a non-permeable surface so air-borne defense signals from one group do not elicit a response in another. In the herbivory treatment group, M. sexta will be allowed to feed for a 24-hour period to account for diurnal and nocturnal herbivory, as well as high plant enzymatic response. Damage treatment 1 will simulate herbivory using a hole-punch, avoiding the midvein. Damage treatment 2 will simulate herbivory using a single transverse cut across the leaf blade and midvein. The undamaged control will have no herbaceous or mechanical damage done. After the treatment period, half of the leaf matter from all groups will be collected and frozen in liquid nitrogen to preserve all metabolic processes and GCMS will be used to analysis metabolite differences. The other half of the leaf matter will be analyzed using UV-spectrophotometer analysis with bromocresol green to compare the glycoalkaloid expression between the treatments. It’s expected that there will be a reduced expression of the defense glycoalkaloids in the herbivory group of S. lycopersium when compared to the two defoliation groups. While the three treatments could respond similarly to their treatments by primary metabolite expression, the type of defoliation could have a greater effect on this than the herbivory group.

Authors: Thomas Caldwell. Mentors: Dustin Shipp. Insitution: Utah Valley University. In this research project, we have transformed an existing Raman spectroscopy simulation, enhancing its performance and capabilities through the integration of parallel computing with GPU acceleration. This significant improvement in computational time allows us to break through previous computational limitations, enabling more sophisticated and complex applications of the simulation. We are currently focused on two main objectives: assessing the viability and potential of spatially offset Raman spectroscopy (SORS) for deeper tissue analysis, and exploring the possibilities of topographical imaging using Raman techniques.

Authors: Tyler O'Loughlin, Tyler Daynes, Erik Prazak. Mentors: Dustin Shipp. Insitution: Utah Valley University. In the realm of photon interactions with surfaces, the reflection and refraction of light depend significantly on the micro and macrostructures of the surfaces. Despite the use of various methods to attenuate light, their effectiveness remains a subject of interest. In this study, we delve into the properties that render super-black coatings, such as Vantablack, exceptionally dark compared to coatings with distinct surface characteristics but significantly altered amplitudes of reflected intensity. Our research focuses on understanding the optical properties of Vantablack; in particular, we investigate the role of the carbon nanotube microstructure in achieving its unique light-absorbing properties. Through a comprehensive analysis of our samples, we have uncovered that the carbon nanotube microstructure of Vantablack contributes to its remarkably low reflective intensity and exceptional absorption across all wavelengths of light. Notably, Vantablack displays such a high optical density that even at Brewster's angle, a reflection is virtually undetectable. This research sheds light on the intriguing properties of Vantablack and has implications for a wide range of applications where light absorption and reflection play a critical role.

Authors: Jaylee Montague, Skylar Belka, Gabe Espinoza, Geoff Zahn, Alma Laney. Mentors: Alma Laney. Insitution: Utah Valley University. In May of 2022, a several unidentified trees on Utah Valley University (UVU) campus property exhibited severe virus-like symptoms including: mosaic patterning; yellowing, curling, deformed leaves; and flaking bark. Due to the presence of unidentified aphids and after a literature search, we began investigating the sample for a +ssRNA virus. Extensive testing and RNA sequencing using the MinION v9.4.1 and the cDNA PCR Barcoding kit revealed that neither a ssRNA nor a dsRNA virus were present in the samples, thus, it was suspected that a DNA virus could be the cause. We utilized Rolling Circle Amplification (RCA) on extracted DNA. Following the RCA, gel electrophoresis revealed a strong amplicon larger than 10 kb, suggesting the presence of a DNA virus. Sequencing of the viral genome from the RCA product using the MinION vs10.4.1 chip showed that the mulberry did contain baculoviruses and large DNA viruses. Unfortunately, though, the MinION did not get results for DNA plant viruses, so we sent samples for RNAseq on the Illumina NovaSeq, with results still pending. This is significant since it is likely that the other symptomatic plants near these trees—including redbud and spirea—were infected with the same virus due to RCA amplification in those samples and the presence of the same potential aphid vector. Mechanical inoculations to several herbaceous hosts were done using infected spirea tissue. Furthermore, to our knowledge this is the first report of a DNA virus infecting spirea and redbud in the United States.

Authors: Joshua Stubbs, Annette Lewis, Kendall Holcomb, Barbara Suassuna Schincariol. Mentors: Geoffery Zhan. Insitution: Utah Valley University. Does Greater Phylogenetic Distance Affect Competition Outcomes in Fungal Communities?Annette Lewis, Kendall Holcomb, Bárbara Suassuna Schincariol, Josh Stubbs, Geoffery Zahn PhDFungi play a critical role in decomposition, affecting nutrient cycling at a global scale. Saprotrophic fungi competitively decompose dead organic matter. However, the role of phylogenetic relatedness on interspecific competition in fungal communities has not received much attention. The theory of phylogenetic over-dispersion suggests that species within a community tend to be less related than expected by chance, therefore limiting competition due to functional redundancy. Similarly, Darwin’s naturalization hypothesis suggests that taxonomically distinct invaders might experience reduced competition and resistance. In this study, we chose three different saprotrophic fungal species with varying relatedness: Aspergillus niger and Fusarium keratoplasticum (from the same family), and Pleurotus ostreatus (from a different phylum). These species were chosen based on decomposition abilities and phylogenetic distances. These species were cultured and placed in seven combinations to assess their ability to decompose and compete as individual fungal populations and as combined communities (e.g., A, B, A+B, B+C, and A+B+C). Each species was placed near a sterilized piece of paper such that competition was evaluated by analyzing the paper coverage in Petri dishes over three weeks. Each Petri dish was analyzed individually based on the average percentage of paper covered and, within combined communities, the percentage of paper each species covered. Interactions between each species and the percentage of the paper covered was recorded for further analysis. Assessing paper coverage allows for the observation of any potential competitive inhibition of decomposition. We hypothesize that decomposing and competitive abilities would be the strongest with A. niger. Despite the fast growth rate of A. niger individually, preliminary results suggest that it was outcompeted when paired with other species. This research highlights the potential nuances in fungal community interactions influenced by phylogenetic relationships, shedding light on the principles of phylogenetic overdispersion and Darwin’s naturalization hypothesis.

Authors: Cristina Chirvasa, Tyler Williams, Matteo Petit Bon. Mentors: Karen Beard. Insitution: Utah State University. Arctic regions are experiencing climate change impacts at disproportionately high rates. Rising temperatures, flooding from melting sea ice and increasing storm surges, and changing animal migration patterns are all contributing to altered Arctic ecosystems. Here, we examine the impact of warming, flooding, and increased herbivory on three plant communities, with varying levels of background inundation due to their distance from rivers, in the Yukon-Kuskokwim (Y-K) delta, a large riverine delta in Alaska. While previous research has examined climate change responses in the Arctic, there have been few studies on the response of plant functional traits to herbivory with other environmental factors. We will conduct an experiment to measure the impact of these variables (herbivory, flooding, and warming) across three different habitat types. My research will investigate the response of plant functional traits to the experimental treatments. I will fit linear mixed effects models to analyze the impact of our three treatments. This study will increase knowledge on climate change impacts on different plant communities across the Y-K Delta.

Authors: Jacob Atkinson. Mentors: Vinodh Chellamuthu. Insitution: Utah Tech University. Insurance is a crucial part of economies worldwide, paying billions of dollars in claims yearly. Insurance companies need to anticipate future claim liabilities to manage the high volume of claims. This research investigates the use of linear and non-linear machine learning algorithms, including linear regression, ridge, lasso, elastic net, Decision Tree Regressor, Random Forest Regressor, and Gradient Boosting Regressor, to predict auto insurance claim amounts. The performance of each model is assessed using various metrics, such as mean squared errors, mean absolute errors, and R-squared. An optimized model will also be used to estimate the future financial impact.

Authors: Baylee Christensen, Reagan Mckee, Dante Celani, Candice Johnson, Randy Klabacka. Mentors: Randy Klabacka. Insitution: Utah Tech University. When studying the genetics of biological systems, we often assign individuals to categories (e.g., “ecotypes”) and then assess genetic differences using computational biology tools. If populations within categories are used as units in statistical models, this can present a potential statistical pitfall called pseudo-replication (which happens when multiple measurements of the same population are compared). To circumvent this flaw, we developed a software package called CatPop. CatPop considers all possible population assignments (using combinatorics) and performs a permutation test to determine whether a locus has significantly greater divergence between groups compared to within the same group. Our test of CatPop on simulated data shows that it can accurately identify divergent loci between categories, and we also examine its utility with previously-published datasets that examine divergence between ecotypes.

Authors: Haley LeFevre, Julie Pynn. Mentors: Julie Pynn. Insitution: Southern Utah University. Loneliness is a perceived isolation resulting from a lack of meaningful social connections. The COVID-19 pandemic increased rates of loneliness for many people, including college students. At the same time, internet use also went up. As loneliness and internet use increased, many people turned to parasocial relationships, forming one-sided emotional connections with online content creators and celebrities. Do students at SUU use parasocial relationships to cope with loneliness? We predict that students at SUU who are experiencing loneliness will be more likely to engage in parasocial relationships. Understanding how people interact with online content and develop relationships with online personalities is essential for promoting digital literacy and positive online behavior as internet usage grows. This research can explain the significance of digital media in students' lives if SUU students are using parasocial relationships to deal with loneliness. In that case, the university can create specialized support programs, workshops, or counseling services to assist students in making more effective coping strategies and minimizing feelings of isolation. Results are forthcoming.

Authors: Atira Schulte. Mentors: Michael Popich. Insitution: Westminster College. We live in an environmental crisis caused by mass overconsumption and non-sustainable practices of humans- issues rooted in anthropocentric beliefs that humans may use all other life to our benefit with no regard for consequences. Animals and ecosystems have the right to exist and flourish, they have intrinsic worth, so they ought to have moral standing. This research aimed to build a new ethical theory that would extend the same rights humans are widely considered to have to other animals and ecosystems; especially citing Martha Nussbaum, James Sterba, and John O’Neill. The age-old desire to dominate nature must be replaced with a newfound respect and wonder for non-human life and the environment; Reframing our methods of thinking and moving towards more sustainable methods of living. Only by changing the mindset of humans, from one of domination and exploitation to stewardship and coexistence-even friendship, can we resolve this environmental crisis.

Authors: Tyler Randall. Mentors: Joshua Price. Insitution: Southern Utah University. This research paper investigates the relationship between men's consumption of pornography and its potential influence on women's decisions regarding marriage. In an era of increasing acceptance of pornography, this study delves into the socio-economic implications of its widespread usage. The research builds upon existing literature that suggests individuals turn to pornography as an escape from negative emotional state. As internet pornography has grown into a big industry, we aim to understand its impacts not only individuals but also on society. Numerous studies have found that pornography as a factor contributing to divorce cases. Specifically, women may perceive pornography as a form of infidelity, which is shown to lead to unhappiness and potentially divorce. Our research contributes to the field by examining the distinct needs and motivations of men and women in the context of marriage. We seek to determine whether men's pornography consumption leads women to view them as less suitable for marriage. We explore the possibility that men may be giving up their ability to be an attractive option for women if they are looking at pornography.

Authors: Joshua Booher. Mentors: Jacob Hickman. Insitution: Brigham Young University. Musicians in Northern Ireland described the space and community that they were actively trying to build in Belfast as “the Third Thing”. In Belfast, music has remained as an alternative third space to the sectarian dualism of Protestant vs Catholics. Since before the Easter Rising in 1916 Catholic Nationalists have fought to obtain sovereignty by establishing a United Ireland while Protestant Unionist fight to retain what they have obtained and defend king and country from Nationalist siege. Their opposition to each other fueled The Troubles in Belfast. Since signing the good Friday agreement in 1998, active tension between these communities still creates further divides and oppositions toward their counterparts. The Good Friday Agreement ostensibly marked the end of the Troubles by providing equal sovereignty to both sides. In Belfast, music has played a significant role in anti-sectarian thirdness. Punk fostered this thirdness within the conflict of the Troubles through its references by way of its anti-sectarian framework. In contemporary Belfast, the residual punk scene now serves as one genre of many in the broader music scene. This music scene has now been self proclaimed as this “third thing” which pushes back on implosive sectarian frameworks between Catholic and Protestant communities.

Authors: Grace Collier, Sadee Ball, Shelby Shepherd, Madi Kotter, Sierra Jeppesen, Tanner Wilson. Mentors: Julie Pynn. Insitution: Southern Utah University. ABSTRACTA large body of research indicates that nature has a positive effect on anxiety levels and overall well-being. This has been tested in many different ways, including through the use of soundscapes. This study aims to analyze how different soundscapes—nature sounds, urban sounds, and white noise—impact anxiety levels. Furthermore, it aims to determine whether or not this relates to one’s perception of origin. Quantitative results indicated that nature sounds and white noise tended to reduce anxiety levels the most, with urban sounds having an insignificant effect. Qualitative results revealed that nature sounds evoked the highest rate of positive emotion, with the white noise soundscape having the second highest rate, and the urban soundscape having the lowest rate. Keywords: soundscapes, nature sounds, urban sounds, white noise, anxiety, perception of origin

Authors: Hunter Gordon. Mentors: Geoff Smith. Insitution: Utah Tech University. Precipitation and temperature represent two of the most critical and extensively studied abiotic factors that impact ectotherms. With climate change, regions already under stress are experiencing heightened heat and aridity, which is forcing populations to adapt or face extinction. Scalation patterns, which may vary in response to these abiotic factors, could hold valuable insights for predicting the future. This study focuses on Side-blotched lizards, specifically Uta stansburiana, sampled from various populations to assess the influence of local climatic factors on their scalation. Additionally, museum specimens are integrated into the analysis to discern how these patterns have evolved over time, possibly in response to shifting climatic conditions. Through the examination of multiple locations spanning different time periods, we aim to enhance our ability to forecast the adaptive responses of organisms in the face of the hotter and drier future predicted by climate models for the American Southwest.

Authors: Alyssa Taylor. Mentors: Dr. Marquez-Velarde . Insitution: Utah State University. Athletics United (AU) is a non-profit organization in Logan, Utah dedicated to using “sport to provide a supportive environment for new American families to integrate with local communities for personal, physical and social growth” (https://www.athleticsunited.us/). To this point, AU lacks meaningful research to measure its progress in meeting its mission statement. In order to qualify and quantify AU’s impact, this project investigates question: “Does Athletics United help families integrate into local communities and how?” Athletics United integrates families into the community by providing a space to build friendships, expand one’s world view, and access community resources. During Summer of 2023, nine families participating in AU were interviewed. A mix of American and new American families were recruited. Four interviewees required an interpreter. Interviews are being qualitatively analyzed using NVivo. The interview data confirms that Athletics United integrates families into the community by providing a space to build friendships, expand one’s world view, and access community resources. To further improve, AU can consider assigning volunteers to help new American families participate in events and access resources more consistently.

Authors: Chance Witt, Devan Hill. Mentors: Jianlong Han, Seth Armstrong, Sarah Duffin. Insitution: Southern Utah University. We study the steady state solutions of a Lotka-Volterra model with crowing effects when delta=2. A nonstandard numerical scheme is proposed, and numerical experiments predict the long term behavior of the numerical solution.

Authors: Kennley Cook. Mentors: Crystal Koenig. Insitution: Southern Utah University. This research examines a brief history of pornography and then the porn industry itself. Specifically what has been driving men and women to perform lewd acts in front of a camera. It explores the mental, emotional, and social impacts that porn stars face from their line of work. The early days of the porn industry were permissive with acts that are considered highly immoral by today’s standards. Early porn included bestiality, child sex abuse, and highly violent pornography. While such acts have been illegalized, the industry continues to be exploitative. The ease of exploitation in the industry is largely driven by the stigma which pornography carries. Those who perform in pornographic settings are often shamed so much that it drives them to stay in the industry long after they may have wished to depart. While porn has always been taboo, it’s become far more taboo in recent years which has left many workers in the industry unprotected and open to abuse. Despite many of the dangers of pornography, porn itself is not inherently evil. In proper settings, pornography can be helpful for individuals in coming to terms with their sexuality. It can also be used to help couples equalize libidos and form bonds. It can also be liberating for some performers, with some research even suggesting porn stars have higher self esteem than those not in the porn industry . The dangers of pornography come about due to lack of regulation and outside shaming forces. To truly minimize the harms of pornography (and maximize its benefits), it’s crucial that the stigmas surrounding it be reduced so that laws and regulations may be enforced to protect its performers and viewers.

Authors: Hunter Gordon, Jace Riley, McCade Larsen. Mentors: Aaron Davis. Insitution: Utah Tech University. Tracking animal populations is key to ensuring that populations are healthy and thriving. Current data collection methods, such as radio tagging, manned aerial flyovers, and camera traps, are not only time-consuming and expensive but also fail to provide accurate population estimates. This interdisciplinary research project aims to produce a more accurate and less expensive data collection system for large game populations that could potentially be used to monitor other animal populations. The planned procedure for data collection is to attach a thermal imaging device to an unmanned aerial vehicle (UAV) and perform aerial transects throughout the observation area. Using a thermal camera will allow the UAV to fly at a higher altitude, lessening the disturbance to the animals themselves. The imaging device will take images at a set time interval along transects that can be stitched into a complete data set for the area. By using image processing techniques and deep learning models, the images will be processed to show the location as well as population counts of the animals in the area. Results from similar experiments have shown that using UAVs to collect population data not only provides more accurate data but also requires less time overall to collect the data.[1] This experiment expands upon those findings by creating automatic image processing and analysis software to increase the ease of use, allowing for more data to be collected and analyzed in the same time period.

Authors: Joshua Parmley, Acacia Overono. Mentors: Acacia Overono. Insitution: Utah Valley University. Since its founding over a century ago, the American Psychological Association has adopted several distinct roles in its ongoing pursuit of legitimacy, stability, and growth for the field of psychology. Two of its most important roles include the establishment and enforcement of an ethics code and the standardization and development of undergraduate education. These two goals are related; the best way to uphold an ethics code is to ingrain that code into future researchers and practitioners. The APA Guidelines for the Undergraduate Psychology Major 3.0, which lists the APA’s ideal learning objectives for undergraduate programs, attempts to do just that. In its section on ethics training, it places a great degree of responsibility on undergraduate programs to prepare students for a world of bad actors, exploitative systems, and ethical grey areas. To achieve this, the document recognizes that students must develop an ethical skill or proficiency, not just a knowledge of the codes and principles. Unfortunately, Guidelines recommendations for achieving that goal quickly become clouded, as the document wrestles with the various concerns about teaching ethics. Primarily, the Guidelines section on ethics seems conflicted about whether instructors should give students historical examples of psychologists and psychology systems engaging in unethical behavior The Guidelines expresses the concern that presenting students with examples of ethical failures by psychologists will distract students from the learning outcomes or lead them to view the field negatively and abandon it altogether. Accordingly, the APA tentatively recommends that negative examples be shared cautiously, alongside examples of the positive achievements of the field and ethical systems in place. Are the APA’s fears that blunt ethics courses could turn students away from the major valid? Or do the Guidelines recommendations put too little faith in their instructors and students? This study seeks to examine the validity of the APA’s concerns and the efficacy of their recommendations. One hundred and fifty Intro to Psychology students will be divided into three groups. Each group will be instructed in the principles of the APA ethics code using a) no historical examples, b) a balance of negative and positive examples, or c) several negative examples. Participants will then take a survey that measures their ethical proficiency, their memory of the material, and their confidence in the field of psychology. Measurement of ethical proficiency will be adapted from APA measurement tools. We predict that the group instructed with several negative examples, without balancing positive examples, will result in greater measurements of ethical proficiency, better recall, and a slightly lowered level of confidence in the field. We predict this based on the idea that reviewing negative examples in the instructional material will provide students with a specific model of ethical decision-making.

Authors: Matthew Hovorka. Mentors: Connie Ericksen. Insitution: Utah Valley University. This research project examines evidence of individual identity and personhood among perinates, neonates, and young children in the Tiwanaku culture (Western Bolivia, Chile, Peru, A.D. 500-1100) of the ancient Andes. A multifocal approach is taken in examining the topic, and ethnohistorical and archaeological evidence is examined to create a picture of Tiwanaku conceptions of personhood. Through a comprehensive analysis of burial practices, iconography, and ethnohistorical records, this study sheds light on the nuanced conceptions of personhood and individual identity held within the Tiwanaku culture. By delving into the socio-cultural context and material evidence, this research contributes to a deeper appreciation of the complex beliefs and societal values of this ancient Andean civilization.

Authors: Ayana Srivastava, Gliceida Galarza Fortuna, Beverly Chigarira, Emre Dal, Chadi Hage Chehade, Georges Gebrael, Arshit Narang, Neeraj Agarwal, Umang Swami, Haoran Li. Mentors: Neeraj Agarwal. Insitution: University of Utah. The utility of ChatGPT in subspecialty consultation for patients (pts) with metastatic genitourinary (GU) cancer.Ayana Srivastava, Gliceida Galarza Fortuna, Beverly Chigarira, Emre Dal, Chadi Hage Chehade, Georges Gebrael, Arshit Narang, Neeraj Agarwal, Umang Swami, Haoran LiHuntsman Cancer Institute at the University of Utah, Salt Lake City, UT;University of Kansas Cancer Center, Westwood, KSBackground:Cancer management requires a multidisciplinary approach, often requiring medical consultation from subspecialists. With the advent of artificial intelligence (AI) technologies like ChatGPT, it is hypothesized that these tools may help expedite the consultation process. This study aimed to assess the efficacy of ChatGPT in providing guideline-based sub-specialty recommendations for managing pts with metastatic GU cancer.Methods:In this single-institution, IRB-approved, retrospective, proof-of-concept study, pts with metastatic GU cancer over the past 3 years were screened. Those with at least one consultation referral to subspecialty clinics were randomly selected. ChatGPT 3.5 was given the most recent clinic note that triggered sub-specialty consultation. The AI tool was then asked to provide an assessment and plan. Two physicians independently assessed the accuracy of diagnoses made by ChatGPT and subspecialty physicians. The primary outcome was the consistency of ChatGPT recommendations with those of subspecialty physicians. Secondary Outcomes included potential time saved by using ChatGPT and comparison of medical decision-making (MDM) complexity levels between ChatGPT and subspecialty physicians.Results:A total of 39 pts were included. Their primary diagnoses included prostate cancer (51.3%), bladder cancer (23.1%) and kidney cancer (15.4%). The referred subspecialty clinic included cardiology (33.3%), hematology (17.9%), hepatology (2.6 %), hospice (10.3%), neurology (12.8%), pulmonary (15.4 %), and rheumatology (7.7%). The average waiting time for pts to be seen in subspecialty clinics was 44.9 days (SD = 42.4). Of 39 patient’s charts reviewed by ChatGPT, 30/39 (76.9%) had the same diagnosis with consultant sub-specialties. The average diagnoses made by ChatGPT were 8.2, compared with 3.4 made by sub-specialty physicians (p < 0.0001). The accuracy of diagnoses made by ChatGPT was the same, higher, and lower than human physicians in 10 (33.3%), 3 (10%), and 17 (56.7%) cases, respectively. Consistency of treatment plans between ChatGPT and physicians was found in 18 cases (46.2%). ChatGPT recommended additional workup in 32 cases (85.1%). The average number of words written in consultation notes by ChatGPT was 362.7 (SD = 72.9), which was significantly greater than subspecialty physicians (n = 224.7, p < 0.0001).Conclusions:These hypothesis-generating data suggest the potential utility of ChatGPT to assist medical oncologists in managing increasingly complex pts with metastatic cancer. Further studies are needed to validate our findings.

Authors: Patrick R Davies II. Mentors: Acacia Overono. Insitution: Utah Valley University. While there is a dedicated area of the brain for recognizing faces, the efficacy of recognition tasks can vary depending on the context under which they’re performed. The existing literature is undecided on whether anxiety helps or harms tasks relating to emotional content recognition, and some studies have taken steps into researching memory as an additional layer to these recognition tasks. A preliminary study was performed with 16 participants using virtual reality to study the effects of acute anxiety on the ability to correctly identify faces. Participants played either a brief horror game or a brief virtual sandbox in order to induce a desired emotional state, then were asked to complete a brief paired-face identification paradigm. Initial results show that anxiety strengthens identification of neutral emotionally-valent face pairs. Further results may clarify the relationship between anxiety and face identification processes.

Authors: Jared Barrot, Mackenzie Burr, Isaac Packer, Peyton Worley, Delaney Anderson, Jack Davis, Jeffrey Okojie, Ken Dixon. Mentors: Jared Barrott. Insitution: Brigham Young University. In cancer diagnosis, drug testing, and treatment, analysis is done almost exclusively on formalin-fixed paraffin-embedded (FFPE) tissue samples, a process known to cause chemical cross-linking, DNA fragmentation, and degradation. With the push for genetic testing and personalized medicine, cryopreservation of tissue samples has started to gain traction in the research community. We utilized FFPE samples and cryopreserved samples, extracted DNA, and compared the samples using three criteria: DNA purity, quality, and yield. DNA yield, measured on nanograms of DNA to milligrams of tissue basis, demonstrated a five times the yield in cryopreserved samples than in FFPE. DNA purity did not show significant differences between a cryopreserved tissue sample versus a FFPE tissue sample, confirming valid use of FFPE storage methods if purity is the only factor; however, DNA quality was significantly better in cryopreserved tissue samples, with nearly twice the DNA quality number (DQN) than FFPE samples demonstrated, with significant portions of longer base pair DNA fragments present, widening the range of molecular applications of the sample. These cryopreserved tissues provide higher DNA quality, a higher percentage of significantly longer DNA fragments, and increased DNA yield from individual samples, confirming that cryopreservation is a better option than FFPE when it comes to choosing tissue preservation and storage methods. More molecular assays and tests can be run, and smaller samples are necessary when utilizing cryopreservation, proving it to be a better option than the “gold standard” of FFPE, particularly in regards to cancer diagnosis, drug testing, and treatment exploration.

Authors: Danielle Alvarado, Kasidy Weber, Shannon Buckley. Mentors: Shannon Buckley. Insitution: University of Utah. The ubiquitin-proteasome system (UPS) plays a critical role in cancer by regulating the degradation of key proteins involved in cell cycle control, DNA repair, apoptosis, and signaling pathways, making it a crucial mechanism for maintaining cellular homeostasis and preventingtumor development and progression. The expression levels of F-box ubiquitin E3 ligase, FBXO21, correlates with patient survival in several types of cancer, suggesting a role in tumorgenesis. We have previously shown silencing FBXO21 in acute myeloid leukemia (AML) cell lines and patient samples leads to differentiation, slowed tumor progression, increased chemotherapy sensitivity, and altered cytokine signaling. Further, we found that FBXO21 ubiquitinates p85α, a regulatory part of the phosphoinositide 3-kinase (PI3K) pathway, for degradation resulting in altered PI3K signaling. Although PI3K inhibitors are used in cancertreatment, issues like off-target effects, dose-limiting toxicities, and resistance necessitate further research and development for optimized efficiency and minimal negative effects in targeting the PI3K pathway. To further understand the role of FBXO21 in cancer, we utilized shRNAs targeting FBXO21 in various cancer cell lines to identify the role of FBXO21 in cellular proliferation, apoptosis, and alteration of the PI3K pathway. The PI3K signaling pathway is initiated through p85α binding to the catalytic subunit, p110, leading to downstream AKT and mTOR activation. We found knockdown of FBXO21 stabilizes p85α, leading to dimerization of free p85 and decreased PI3K pathway activation. Therefore, we hypothesize that FBXO21 may function as a novel regulator of PI3K signaling, offering an alternative approach to PI3K inhibitors. Our findings suggest that FBXO21 may represent a novel therapeutic target acrossvarious cancer subtypes to target the overactive PI3K pathway. Targeting FBXO21 may enhance the efficacy of current therapies and provide potential benefits for improving patient outcomes in the treatment of cancer, including but not limited to AML

Authors: Jude Werth. Mentors: Alex Shcheglovitov. Insitution: University of Utah. Glioblastoma (GBM) is the deadliest brain tumor that affects more than 10,000 people a year. Unfortunately, our understanding of the mechanisms disrupted by GBM is extremely limited. We are unable to safely manipulate brain cells in live patients, and the complexity of human brain networks are difficult to recapitulate in animals. This study employs brain organoids, derived from induced Pluripotent Stem Cells (iPSCs), to model the impact of GBM on neural activity. Organoid batches, grown under normal conditions and co-cultured with GBM cells, were monitored using microelectrode arrays (MEA) to record power in frequency bands. Over time, drugs influencing neural activity were introduced. Significant behavioral distinctions were observed between control and GBM-cultured organoids. Under Bicuculline and Tetrodotoxin, power in GBM organoids exhibited dramatic changes compared to the minimal difference in control organoids. Conversely, 4-Aminopyridine induced increased high-frequency activity exclusively in control organoids. Correlating power in frequency bands with spike activity, this study provides insight on how brain tumors influence neural networks, furthering disease comprehension to eventually develop effective treatment for patients.

Authors: Trenton M. Gibson, Brett E Pickett. Mentors: Brett E Pickett. Insitution: Brigham Young University. Lynch Syndrome, formerly known as Hereditary Non-Polyposis Colorectal Cancer, is an autosomal dominant condition characterized by non-functional DNA mismatch repair genes, leading to mismatch repair deficiency (dMMR). Individuals with dMMR have an increased risk of developing cancerous tumors with microsatellite instability (MSI). The aim of this study was to investigate significant perturbations in gene expression among different dMMR variants within Lynch Syndrome, specifically focusing on variants in the PMS2, MLH1, and MSH2 loci. We obtained 55 public RNA-seq human colorectal tumor samples with specific germline dMMR mutations and 20 samples of healthy colorectal epithelium from the GENE Expression Omnibus (GEO). These samples were subjected to a robust RNA-seq data preprocessing and analytical workflow, which involved quality control, trimming, read pseudomapping and quantification using Salmon, identification of significant differentially expressed genes using edgeR, and gene ontology enrichment analysis using Camera. Signaling pathway impact analysis (SPIA) was employed to assess gene pathway perturbations. Our results revealed that tumor samples from individuals with germline mutations in the PMS2 MMR gene exhibited transcriptomic profiles indicating upregulation in pathways related to intrinsic and extrinsic prothrombin activation, fibrinolysis, and uPA/uPAR-mediated signaling, in comparison to other tumor and healthy samples. Previous research has established a correlation between these pathways and tumor growth, invasiveness, and metastasis. These findings provide a foundation for further research to explore the interactions between PMS2 and these pathways in influencing tumor development.

Authors: Bryce Tolman, Jasper Terungwa Iorkula , Justin Singleton, Matt Peterson. Mentors: Matt Peterson. Insitution: Brigham Young University. An efficient method for preparing C-3 aminated pyrazolo[1,5-a]pyrimidines was developed. The method consisted of treating 3-bromopyrazolo[1,5-a]pyrimidine with a wide range of 1° or 2° alkylamines with CuI (20 mol%), L-proline (40 mol%), and Et 3 N (2 equiv) in DMSO under microwave heating conditions (130 °C, 4 hours). C-3 aminated products were obtained in good to excellent isolated yields (54–90%; ave. yield = 77%), and represents the most broadly applicable, non-palladium-catalyzed amination of 3-bromopyrazolo[1,5-a]pyrimidine ever reported. The coupling worked well for unfunctionalized 1° alkylamines such as butylamine, benzylamine, isopropylamine or cyclohexylamine, and more densely functionalized 1° amines with CH 2 - or CH 2 CH 2 -linked heterocycles were also well tolerated (e.g. pyridine-, indole-, thiophene-, and furan-linked methyl- or ethylamine derivatives). 2° Alkylamines such as morpholine, pyrrolidine, and piperidine also reacted well under these conditions. Heating under conventional oil-bath conditions required a more extended reaction time (40 h), but also gave products in good yield (72-76%) for both 100-mg and gram-scale reactions. A pegylated azide (11-azido-3,6,9-trioxaundecan-1-amine) was also coupled in good yield (65% yield microwave), demonstrating compatibility of this methodology for potential click-type applications. Our method is simple and efficient, and avoids drawbacks associated with alternative methods, including long reaction times, limited substrate scope, and required use of air-sensitive/extremely expensive palladium catalysts and specialized synthetic ligands.

Authors: Natalia Rubio, Madison Farrer. Mentors: Christopher Anderson. Insitution: Utah Valley University. Functional Near-Infrared Spectroscopy (fNIRS) is a neuroimaging technique used to monitor brain activity by measuring the changes in blood oxygenation levels, especially when presented with stimuli or given tasks. This is due to the principle that active brain regions require more oxygen, resulting in changes of blood flow and concentrations of hemoglobin. These observable results are achieved by diodes placed on the surface of the scalp that emit near-infrared (NIR) light into the skull. The absorption of this light by the hemoglobin in the brain’s blood vessels is then detected, localizing neural activity. Due to its portability and cost-efficient nature, fNIRS is being widely used across various fields of research and clinical applications whereas other neuroimaging methods might be less feasible or practical. Psychologists and neuroscientists are able to utilize fNIRS to understand how different regions of the brain are involved in various cognitive processes. It has been used in studying conditions like Alzheimer's disease, stroke, traumatic brain injury, autism, schizophrenia, mood disorders, and dementia. In the context of dementia research, fNIRS has emerged as a promising tool . While fNIRS has a low spatial resolution with measurements restricted to the outer cortex of the brain, it enables the study of the progression of dementia by providing real-time data and offering relative tolerance to subject motion. In fact, a neuro-clinic in Provo, Utah (Cognitive FX) has been studying the use of this fNIRS technique on patients with dementia while they perform language tasks. Although no patient data will be used in this study, the use of fNIRS as a technique to learn about the progression of dementia is noteworthy. Therefore, following IRB approval I will perform semi-structured qualitative interviews with two neuro-technicians using fNIRS in this clinic. Items will focus on the strengths and challenges of using fNIRS with dementia patients. Additionally, I will shadow these technicians to gain hands-on experience. Qualitative results will be analyzed and presented. It is anticipated that this research project will raise awareness of the use of fNIRS as a technique in neuroscience as well as its potential to benefit those with dementia.

Authors: Ellie P. Evans, Mmadili Ilozumba, Sheetal Hardikar, Neli Ulrich, Jennifer Ose. Mentors: Mmadili Ilozumba. Insitution: Utah Valley University. Cachexia is a complex syndrome characterized by severe weight loss and muscle wasting. It is also a prevalent complication in various chronic diseases, including cancer and acquired immunodeficiency syndrome. Recent research has underscored the pivotal role of the gut microbiome in systemic well-being. Furthermore, it has been shown that cancer patients suffering from cachexia have unique bacteria composition in the gut compared to that of non-cachectic patients. In the absence of stool samples, there are a few metabolites in the circulatory system that can be directly linked to gut microbiota. Examples include bile acids, and short chain fatty acids as metabolites produced by specific Phylum. The presence of these gut microbiota derived metabolites (GMDM) in the circulation indicate a compromise in the integrity of tight junctions in the intestines. These results prompted an investigation of GMDM as an indicator of gut permeability. Our hypothesis is that an increased concentration of GMDM in the circulation is linked to gut permeability, leading to the development of cachexia in patients with colorectal cancer.  This literature review provides a comprehensive analysis of the relationship between gut permeability and cachexia, seeking to identify which GMDMs are the most accurate indicators of specific bacteria linked to gut permeability, and subsequently, are accurate projectors of cachexia.  Acetic acid, a short chain fatty acid (SCFA) is a GMDM detected in the plasma of patients with gut permeability. Acetic acid makes up about 60% of the SCFAs produced by a healthy gut flora and 90% of it is produced by the genus Bifidobacterium. Our literature review will cover how closely a measurement of acetic acid and other GMDMs measured in plasma can be linked to gut permeability and to Bifidobacterium levels in the gut. Similarly, Indole is a GMDM produced by Escherichia coli and other indole-producing bacteria. Indole isn’t produced naturally anywhere else in the body. When it is naturally absorbed into the liver through the portal vein, it’s mostly converted into indoxyl sulfate. The heightened presence indole in plasma in sulfate-free forms could indicate that indole is being absorbed through holes in the tight junctions of the intestines, rather than through the portal vein. This information would allow cohorts that have collected plasma samples in the past to measure gut permeability by proxy, even if they’re unable to investigate the gut microbiota and metabolites present from stool samples. These connections found could further help us to use-already collected samples to find accurate biomarkers for early cachexia development in cancer patients. If an oral presentation is unavailable, a poster presentation would be great.

Authors: Isabella Scalise. Mentors: Conan Kinsey. Insitution: University of Utah.

Authors: Ana Paulina Medellin-Alvarez, Jared Almazan, Tursun Turapov, David A. Kircher, Karly A. Stanley, Gennie L. Parkman, MiKaela N. Field, Katie M. Culver, Silvia Coma, Jonathan A. Pachter, Howard Colman, Sheri L. Holmen. Mentors: Sheri Holmen. Insitution: University of Utah. Despite promising results from recent FDA-approved therapies, many advanced melanoma patients develop resistance to both immunotherapy and targeted therapy. A common resistance mechanism to targeted therapy is upregulation of the PI3K/AKT signaling pathway, which has also been shown to promote the development of melanoma brain metastases. Historically, AKT inhibitors have failed in the clinic due to their limited efficacy or intolerable toxicity. Proteomic analysis comparing non-metastatic vs brain metastatic primary tumors in mice revealed focal adhesion kinase (FAK) as an AKT1 specific effector and a potential alternative therapeutic target. FAK is a non-receptor tyrosine kinase that localizes primarily to focal adhesions to regulate cell migration. To determine whether targeting FAK alone or in combination with the RAF/MEK inhibitor avutometinib reduces brain metastases and prolongs survival, we utilized both autochthonous and syngeneic melanoma mouse models. Mice with either subcutaneous tumors or established brain metastases were treated with FAK inhibitor, RAF/MEK inhibitor, or the combination of FAK and RAF/MEK inhibitors. Each cohort was assessed for tumor onset, growth, metastasis, and survival. Our results show that combined RAF/MEK/FAK inhibition significantly delays tumor onset, causes regression of established tumors, prevents the development of brain metastases, promotes the regression of established brain metastases, and prolongs survival. In addition, patient-derived BRAF V600E melanoma xenograft mouse models resistant to the BRAF inhibitor dabrafenib and the MEK inhibitor trametinib, were sensitive to combined RAF/MEK/FAK blockade. The addition of the BRAF inhibitor encorafenib to these models further enhanced the effect on tumor growth. These results support the initiation of a clinical trial evaluating the efficacy of the RAF/MEK inhibitor avutometinib in combination with the FAK inhibitor defactinib in patients with brain metastases from cutaneous melanoma. Additionally, we are assessing non-canonical roles of FAK in modulating the tumor microenvironment to determine whether avutometinib and defactinib also enhance the efficacy of immune checkpoint inhibition in this disease.

Authors: Matthew Stecher, Terungwa Iorkula, Matt A. Peterson. Mentors: Matt A. Peterson. Insitution: Brigham Young University. Tropomyosin Receptor Kinase (TRK) inhibition has become an effective therapeutic approach for treatment of a variety of cancers including soft tissue sarcoma, pilocytic astrocytoma, non-small cell lung cancers, colorectal cancers, glioblastomas, pediatric gliomas, papillary thyroid cancers, and secretory breast carcinoma. Despite the successful use of first-generation TRK inhibitors Larotrectinib and Entrectinib which have shown significant therapeutic response in patients, acquired resistance to these drugs has emerged and is creating serious barriers to maintaining long-term therapeutic efficacy. A common mechanism for acquired resistance is associated with the emergence of mutations. Hence, mutation-induced drug resistance continues to be a major challenge for both first- and second-generation TRK inhibitors. Here, we present the design of novel macrocyclic 3,5-diaminated pyrazolo[1,5-a] pyrimidine ligands with high potential for combating drug-resistant disease.

Authors: Corin Marsh, Acacia Overono. Mentors: Acacia Overono. Insitution: Utah Valley University. A variety of memorization techniques have been shown to enhance the ability to recall important information. While many of these techniques focus on repetition and retrieval of verbal information, the drawing superiority effect (DSE, Wammes et al., 2016) is a phenomenon where drawing leads to better recall compared to re-writing. The present study investigates the extent of these findings by combining the DSE with retrieval practice. This experiment utilized a within-subjects, single-blind design consisting of 32 undergraduate students ranging in age from 18 to 42. Participants were tasked with learning 20 vocabulary terms through a guided slideshow, then studied terms through; reading definitions (verbal restudy), viewing images of the definitions (visual restudy), writing definitions (verbal retrieval), and drawing definitions from memory (DSE with retrieval). After a brief distractor task, participants were tested on their recall of the definitions. Finally, subjects completed a shortened version of the Vividness of Visual Imagery Questionnaire (VVIQ) and a funnel debrief. Preliminary analyses using a repeated measures ANOVA revealed that re-reading was the study method that resulted in the highest recall scores (M = .57) compared to all other conditions (F (3, 93) = 14.9, p < .001, ηp2 = .324). Further — VVIQ ability showed no significant correlation with participant scores for any of the study methods. These results indicate that re-reading was a more effective study strategy for memorizing vocabulary terms with a short delay when compared to viewing images, writing definitions, and drawing definitions from memory. Future work will examine how longer delays and providing feedback influence may modulate the benefits of re-reading, drawing, and retrieval as study strategies. Keywords: drawing superiority effect, retrieval practice, memory, VVIQ

Authors: Ty Wilson. Mentors: Amita Kaundal. Insitution: Utah State University. Climate-induced environmental stresses and the increased use of fertilizers to meet the increasing demand for food significantly threaten food production and soil health. In natural environments, microbes are vital to the growth and development of plants. Their presence in the soil allows for enormous flexibility and nutritional availability for plants, particularly those living in harsh conditions. A plant’s microbiome, especially the underground, plays a significant role in its growth and development and mitigates environmental stresses. Idaho and Utah are particularly challenging environments due to extreme temperatures and arid soil. However, despite these difficult environmental factors, the native plants of Utah and Idaho can thrive. Here, we are exploring the rhizosphere microbiome of Shepherdia x utahensis ‘Torrey,’ a hybrid of two highly drought-tolerant buffaloberry species, S. argentea and S. rotundifolia native to the Intermountain West region of the US to isolate plant growth promoting bacteria. In this study, we isolated drought-resistant plant growth-promoting microbes from the rhizosphere of hybrid buffaloberry. Root samples of hybrid buffaloberry were collected from three locations in Utah: the USU campus, Greenville Farm USU, and the USU botanical garden, Kaysville. The rhizosphere was removed from these root samples and pooled together. The bacteria were isolated on five different bacterial growth media twice. The isolated microbes were then purified using the streak plate method. Forty-six unique isolates were selected based on morphological characteristics and tested for four plant growth-promoting traits. Fourteen bacteria could show the ability to fix nitrogen fix, 18 produced a siderophore, 17 could solubilize phosphate, and 11 produced catalases. IAA production, ACC deaminase, and protease activity assays are in progress. All isolates will be identified by 16S rRNA Sanger sequencing.