Fine Arts

Authors: Matthew Hovorka. Mentors: Connie Ericksen. Insitution: Utah Valley University. This research project examines evidence of individual identity and personhood among perinates, neonates, and young children in the Tiwanaku culture (Western Bolivia, Chile, Peru, A.D. 500-1100) of the ancient Andes. A multifocal approach is taken in examining the topic, and ethnohistorical and archaeological evidence is examined to create a picture of Tiwanaku conceptions of personhood. Through a comprehensive analysis of burial practices, iconography, and ethnohistorical records, this study sheds light on the nuanced conceptions of personhood and individual identity held within the Tiwanaku culture. By delving into the socio-cultural context and material evidence, this research contributes to a deeper appreciation of the complex beliefs and societal values of this ancient Andean civilization.

Authors: Ayana Srivastava, Gliceida Galarza Fortuna, Beverly Chigarira, Emre Dal, Chadi Hage Chehade, Georges Gebrael, Arshit Narang, Neeraj Agarwal, Umang Swami, Haoran Li. Mentors: Neeraj Agarwal. Insitution: University of Utah. The utility of ChatGPT in subspecialty consultation for patients (pts) with metastatic genitourinary (GU) cancer.Ayana Srivastava, Gliceida Galarza Fortuna, Beverly Chigarira, Emre Dal, Chadi Hage Chehade, Georges Gebrael, Arshit Narang, Neeraj Agarwal, Umang Swami, Haoran LiHuntsman Cancer Institute at the University of Utah, Salt Lake City, UT;University of Kansas Cancer Center, Westwood, KSBackground:Cancer management requires a multidisciplinary approach, often requiring medical consultation from subspecialists. With the advent of artificial intelligence (AI) technologies like ChatGPT, it is hypothesized that these tools may help expedite the consultation process. This study aimed to assess the efficacy of ChatGPT in providing guideline-based sub-specialty recommendations for managing pts with metastatic GU cancer.Methods:In this single-institution, IRB-approved, retrospective, proof-of-concept study, pts with metastatic GU cancer over the past 3 years were screened. Those with at least one consultation referral to subspecialty clinics were randomly selected. ChatGPT 3.5 was given the most recent clinic note that triggered sub-specialty consultation. The AI tool was then asked to provide an assessment and plan. Two physicians independently assessed the accuracy of diagnoses made by ChatGPT and subspecialty physicians. The primary outcome was the consistency of ChatGPT recommendations with those of subspecialty physicians. Secondary Outcomes included potential time saved by using ChatGPT and comparison of medical decision-making (MDM) complexity levels between ChatGPT and subspecialty physicians.Results:A total of 39 pts were included. Their primary diagnoses included prostate cancer (51.3%), bladder cancer (23.1%) and kidney cancer (15.4%). The referred subspecialty clinic included cardiology (33.3%), hematology (17.9%), hepatology (2.6 %), hospice (10.3%), neurology (12.8%), pulmonary (15.4 %), and rheumatology (7.7%). The average waiting time for pts to be seen in subspecialty clinics was 44.9 days (SD = 42.4). Of 39 patient’s charts reviewed by ChatGPT, 30/39 (76.9%) had the same diagnosis with consultant sub-specialties. The average diagnoses made by ChatGPT were 8.2, compared with 3.4 made by sub-specialty physicians (p < 0.0001). The accuracy of diagnoses made by ChatGPT was the same, higher, and lower than human physicians in 10 (33.3%), 3 (10%), and 17 (56.7%) cases, respectively. Consistency of treatment plans between ChatGPT and physicians was found in 18 cases (46.2%). ChatGPT recommended additional workup in 32 cases (85.1%). The average number of words written in consultation notes by ChatGPT was 362.7 (SD = 72.9), which was significantly greater than subspecialty physicians (n = 224.7, p < 0.0001).Conclusions:These hypothesis-generating data suggest the potential utility of ChatGPT to assist medical oncologists in managing increasingly complex pts with metastatic cancer. Further studies are needed to validate our findings.

Authors: Patrick R Davies II. Mentors: Acacia Overono. Insitution: Utah Valley University. While there is a dedicated area of the brain for recognizing faces, the efficacy of recognition tasks can vary depending on the context under which they’re performed. The existing literature is undecided on whether anxiety helps or harms tasks relating to emotional content recognition, and some studies have taken steps into researching memory as an additional layer to these recognition tasks. A preliminary study was performed with 16 participants using virtual reality to study the effects of acute anxiety on the ability to correctly identify faces. Participants played either a brief horror game or a brief virtual sandbox in order to induce a desired emotional state, then were asked to complete a brief paired-face identification paradigm. Initial results show that anxiety strengthens identification of neutral emotionally-valent face pairs. Further results may clarify the relationship between anxiety and face identification processes.

Authors: Tara Caplin, Tiffanee Suhaka, Maria Balaceanu, Peg Bird, Dannelle Larsen-Rife. Mentors: Dannelle Larsen-Rife. Insitution: Utah Tech University. Early parent-child interactions have a profound impact on the developing brain, influencing lifelong emotional and social well-being. This review evaluates the Newborn Behavioral Observation (NBO), a relationship-based neurobehavioral approach for families and infants three months old and younger. The NBO supports newborns, caregiver’s, and families by providing individualized information on their infants' behavior and competencies. It facilitates communication and intersubjectivity between infants and caregivers during a critical developmental period, while reducing maternal postpartum depression (PPD). Research on the association between the NBO, PPD, and infant neurodevelopment is reviewed and evaluated in high-risk populations. Although evidence supports approaches like the NBO, they are not yet standard in early intervention due to clinicians' lack of training in infant mental health. The NBO is a foundational tool for cultivating positive early relationships with beneficial implications for clinician standard care of newborns and families. Keywords: Newborn Behavioral Observation (NBO), early relationships, postpartum depression (PPD), infant development, relationship-based, early intervention.

Authors: Maria Balaceanu, Dannelle Larsen-Rife, Tara Caplin, Peg Bird, Grace Carsey, Tiffanee Suhaka. Mentors: Dannelle Larsen-Rife. Insitution: Utah Tech University. Attachment theory provides a framework for understanding how early experiences with caregivers influence individuals' emotional and relational development throughout their lives. In addition to understanding how an individual interacts with close others, attachment predicts relationship satisfaction (Gardner et al., 2020). Individuals with secure attachments demonstrate greater adaptability in coping and regulating their emotions, leading to improved socio-emotional well-being. In contrast, those with insecure attachment styles tend to encounter difficulties in socio-emotional adjustment (Gardner et al., 2020). This study will examine how insecure attachment is associated with romantic relationship dissatisfaction, specifically those lasting five years or longer. We focus exclusively on romantic relationships lasting five years or more because in adult relationships, each partner serves as an attachment figure for the other (Diamond et al., 2008). Therefore, spouses have the potential to increase each other's attachment security. This means an initially insecure individual can develop earned secure attachment with their spouse's support. Approximately 300 diverse participants over the age of 18 will be recruited for the study. The Relationship Structures Questionnaire (ECR-RS; Fraley et al., 2011) will assess insecure attachment. The Relationship Assessment Scale (Hendrick, 1988) will assess satisfaction. It is expected insecure attachment will be correlated with lower relationship satisfaction with a romantic partner of five years or longer. Results of the analyses will be presented.

Authors: Jared Barrot, Mackenzie Burr, Isaac Packer, Peyton Worley, Delaney Anderson, Jack Davis, Jeffrey Okojie, Ken Dixon. Mentors: Jared Barrott. Insitution: Brigham Young University. In cancer diagnosis, drug testing, and treatment, analysis is done almost exclusively on formalin-fixed paraffin-embedded (FFPE) tissue samples, a process known to cause chemical cross-linking, DNA fragmentation, and degradation. With the push for genetic testing and personalized medicine, cryopreservation of tissue samples has started to gain traction in the research community. We utilized FFPE samples and cryopreserved samples, extracted DNA, and compared the samples using three criteria: DNA purity, quality, and yield. DNA yield, measured on nanograms of DNA to milligrams of tissue basis, demonstrated a five times the yield in cryopreserved samples than in FFPE. DNA purity did not show significant differences between a cryopreserved tissue sample versus a FFPE tissue sample, confirming valid use of FFPE storage methods if purity is the only factor; however, DNA quality was significantly better in cryopreserved tissue samples, with nearly twice the DNA quality number (DQN) than FFPE samples demonstrated, with significant portions of longer base pair DNA fragments present, widening the range of molecular applications of the sample. These cryopreserved tissues provide higher DNA quality, a higher percentage of significantly longer DNA fragments, and increased DNA yield from individual samples, confirming that cryopreservation is a better option than FFPE when it comes to choosing tissue preservation and storage methods. More molecular assays and tests can be run, and smaller samples are necessary when utilizing cryopreservation, proving it to be a better option than the “gold standard” of FFPE, particularly in regards to cancer diagnosis, drug testing, and treatment exploration.

Authors: Danielle Alvarado, Kasidy Weber, Shannon Buckley. Mentors: Shannon Buckley. Insitution: University of Utah. The ubiquitin-proteasome system (UPS) plays a critical role in cancer by regulating the degradation of key proteins involved in cell cycle control, DNA repair, apoptosis, and signaling pathways, making it a crucial mechanism for maintaining cellular homeostasis and preventingtumor development and progression. The expression levels of F-box ubiquitin E3 ligase, FBXO21, correlates with patient survival in several types of cancer, suggesting a role in tumorgenesis. We have previously shown silencing FBXO21 in acute myeloid leukemia (AML) cell lines and patient samples leads to differentiation, slowed tumor progression, increased chemotherapy sensitivity, and altered cytokine signaling. Further, we found that FBXO21 ubiquitinates p85α, a regulatory part of the phosphoinositide 3-kinase (PI3K) pathway, for degradation resulting in altered PI3K signaling. Although PI3K inhibitors are used in cancertreatment, issues like off-target effects, dose-limiting toxicities, and resistance necessitate further research and development for optimized efficiency and minimal negative effects in targeting the PI3K pathway. To further understand the role of FBXO21 in cancer, we utilized shRNAs targeting FBXO21 in various cancer cell lines to identify the role of FBXO21 in cellular proliferation, apoptosis, and alteration of the PI3K pathway. The PI3K signaling pathway is initiated through p85α binding to the catalytic subunit, p110, leading to downstream AKT and mTOR activation. We found knockdown of FBXO21 stabilizes p85α, leading to dimerization of free p85 and decreased PI3K pathway activation. Therefore, we hypothesize that FBXO21 may function as a novel regulator of PI3K signaling, offering an alternative approach to PI3K inhibitors. Our findings suggest that FBXO21 may represent a novel therapeutic target acrossvarious cancer subtypes to target the overactive PI3K pathway. Targeting FBXO21 may enhance the efficacy of current therapies and provide potential benefits for improving patient outcomes in the treatment of cancer, including but not limited to AML

Authors: Jude Werth. Mentors: Alex Shcheglovitov. Insitution: University of Utah. Glioblastoma (GBM) is the deadliest brain tumor that affects more than 10,000 people a year. Unfortunately, our understanding of the mechanisms disrupted by GBM is extremely limited. We are unable to safely manipulate brain cells in live patients, and the complexity of human brain networks are difficult to recapitulate in animals. This study employs brain organoids, derived from induced Pluripotent Stem Cells (iPSCs), to model the impact of GBM on neural activity. Organoid batches, grown under normal conditions and co-cultured with GBM cells, were monitored using microelectrode arrays (MEA) to record power in frequency bands. Over time, drugs influencing neural activity were introduced. Significant behavioral distinctions were observed between control and GBM-cultured organoids. Under Bicuculline and Tetrodotoxin, power in GBM organoids exhibited dramatic changes compared to the minimal difference in control organoids. Conversely, 4-Aminopyridine induced increased high-frequency activity exclusively in control organoids. Correlating power in frequency bands with spike activity, this study provides insight on how brain tumors influence neural networks, furthering disease comprehension to eventually develop effective treatment for patients.

Authors: Trenton M. Gibson, Brett E Pickett. Mentors: Brett E Pickett. Insitution: Brigham Young University. Lynch Syndrome, formerly known as Hereditary Non-Polyposis Colorectal Cancer, is an autosomal dominant condition characterized by non-functional DNA mismatch repair genes, leading to mismatch repair deficiency (dMMR). Individuals with dMMR have an increased risk of developing cancerous tumors with microsatellite instability (MSI). The aim of this study was to investigate significant perturbations in gene expression among different dMMR variants within Lynch Syndrome, specifically focusing on variants in the PMS2, MLH1, and MSH2 loci. We obtained 55 public RNA-seq human colorectal tumor samples with specific germline dMMR mutations and 20 samples of healthy colorectal epithelium from the GENE Expression Omnibus (GEO). These samples were subjected to a robust RNA-seq data preprocessing and analytical workflow, which involved quality control, trimming, read pseudomapping and quantification using Salmon, identification of significant differentially expressed genes using edgeR, and gene ontology enrichment analysis using Camera. Signaling pathway impact analysis (SPIA) was employed to assess gene pathway perturbations. Our results revealed that tumor samples from individuals with germline mutations in the PMS2 MMR gene exhibited transcriptomic profiles indicating upregulation in pathways related to intrinsic and extrinsic prothrombin activation, fibrinolysis, and uPA/uPAR-mediated signaling, in comparison to other tumor and healthy samples. Previous research has established a correlation between these pathways and tumor growth, invasiveness, and metastasis. These findings provide a foundation for further research to explore the interactions between PMS2 and these pathways in influencing tumor development.

Authors: Bryce Tolman, Jasper Terungwa Iorkula , Justin Singleton, Matt Peterson. Mentors: Matt Peterson. Insitution: Brigham Young University. An efficient method for preparing C-3 aminated pyrazolo[1,5-a]pyrimidines was developed. The method consisted of treating 3-bromopyrazolo[1,5-a]pyrimidine with a wide range of 1° or 2° alkylamines with CuI (20 mol%), L-proline (40 mol%), and Et 3 N (2 equiv) in DMSO under microwave heating conditions (130 °C, 4 hours). C-3 aminated products were obtained in good to excellent isolated yields (54–90%; ave. yield = 77%), and represents the most broadly applicable, non-palladium-catalyzed amination of 3-bromopyrazolo[1,5-a]pyrimidine ever reported. The coupling worked well for unfunctionalized 1° alkylamines such as butylamine, benzylamine, isopropylamine or cyclohexylamine, and more densely functionalized 1° amines with CH 2 - or CH 2 CH 2 -linked heterocycles were also well tolerated (e.g. pyridine-, indole-, thiophene-, and furan-linked methyl- or ethylamine derivatives). 2° Alkylamines such as morpholine, pyrrolidine, and piperidine also reacted well under these conditions. Heating under conventional oil-bath conditions required a more extended reaction time (40 h), but also gave products in good yield (72-76%) for both 100-mg and gram-scale reactions. A pegylated azide (11-azido-3,6,9-trioxaundecan-1-amine) was also coupled in good yield (65% yield microwave), demonstrating compatibility of this methodology for potential click-type applications. Our method is simple and efficient, and avoids drawbacks associated with alternative methods, including long reaction times, limited substrate scope, and required use of air-sensitive/extremely expensive palladium catalysts and specialized synthetic ligands.

Authors: Natalia Rubio, Madison Farrer. Mentors: Christopher Anderson. Insitution: Utah Valley University. Functional Near-Infrared Spectroscopy (fNIRS) is a neuroimaging technique used to monitor brain activity by measuring the changes in blood oxygenation levels, especially when presented with stimuli or given tasks. This is due to the principle that active brain regions require more oxygen, resulting in changes of blood flow and concentrations of hemoglobin. These observable results are achieved by diodes placed on the surface of the scalp that emit near-infrared (NIR) light into the skull. The absorption of this light by the hemoglobin in the brain’s blood vessels is then detected, localizing neural activity. Due to its portability and cost-efficient nature, fNIRS is being widely used across various fields of research and clinical applications whereas other neuroimaging methods might be less feasible or practical. Psychologists and neuroscientists are able to utilize fNIRS to understand how different regions of the brain are involved in various cognitive processes. It has been used in studying conditions like Alzheimer's disease, stroke, traumatic brain injury, autism, schizophrenia, mood disorders, and dementia. In the context of dementia research, fNIRS has emerged as a promising tool . While fNIRS has a low spatial resolution with measurements restricted to the outer cortex of the brain, it enables the study of the progression of dementia by providing real-time data and offering relative tolerance to subject motion. In fact, a neuro-clinic in Provo, Utah (Cognitive FX) has been studying the use of this fNIRS technique on patients with dementia while they perform language tasks. Although no patient data will be used in this study, the use of fNIRS as a technique to learn about the progression of dementia is noteworthy. Therefore, following IRB approval I will perform semi-structured qualitative interviews with two neuro-technicians using fNIRS in this clinic. Items will focus on the strengths and challenges of using fNIRS with dementia patients. Additionally, I will shadow these technicians to gain hands-on experience. Qualitative results will be analyzed and presented. It is anticipated that this research project will raise awareness of the use of fNIRS as a technique in neuroscience as well as its potential to benefit those with dementia.

Authors: Ellie P. Evans, Mmadili Ilozumba, Sheetal Hardikar, Neli Ulrich, Jennifer Ose. Mentors: Mmadili Ilozumba. Insitution: Utah Valley University. Cachexia is a complex syndrome characterized by severe weight loss and muscle wasting. It is also a prevalent complication in various chronic diseases, including cancer and acquired immunodeficiency syndrome. Recent research has underscored the pivotal role of the gut microbiome in systemic well-being. Furthermore, it has been shown that cancer patients suffering from cachexia have unique bacteria composition in the gut compared to that of non-cachectic patients. In the absence of stool samples, there are a few metabolites in the circulatory system that can be directly linked to gut microbiota. Examples include bile acids, and short chain fatty acids as metabolites produced by specific Phylum. The presence of these gut microbiota derived metabolites (GMDM) in the circulation indicate a compromise in the integrity of tight junctions in the intestines. These results prompted an investigation of GMDM as an indicator of gut permeability. Our hypothesis is that an increased concentration of GMDM in the circulation is linked to gut permeability, leading to the development of cachexia in patients with colorectal cancer.  This literature review provides a comprehensive analysis of the relationship between gut permeability and cachexia, seeking to identify which GMDMs are the most accurate indicators of specific bacteria linked to gut permeability, and subsequently, are accurate projectors of cachexia.  Acetic acid, a short chain fatty acid (SCFA) is a GMDM detected in the plasma of patients with gut permeability. Acetic acid makes up about 60% of the SCFAs produced by a healthy gut flora and 90% of it is produced by the genus Bifidobacterium. Our literature review will cover how closely a measurement of acetic acid and other GMDMs measured in plasma can be linked to gut permeability and to Bifidobacterium levels in the gut. Similarly, Indole is a GMDM produced by Escherichia coli and other indole-producing bacteria. Indole isn’t produced naturally anywhere else in the body. When it is naturally absorbed into the liver through the portal vein, it’s mostly converted into indoxyl sulfate. The heightened presence indole in plasma in sulfate-free forms could indicate that indole is being absorbed through holes in the tight junctions of the intestines, rather than through the portal vein. This information would allow cohorts that have collected plasma samples in the past to measure gut permeability by proxy, even if they’re unable to investigate the gut microbiota and metabolites present from stool samples. These connections found could further help us to use-already collected samples to find accurate biomarkers for early cachexia development in cancer patients. If an oral presentation is unavailable, a poster presentation would be great.

Authors: Isabella Scalise. Mentors: Conan Kinsey. Insitution: University of Utah.

Authors: Allison Stevens, Gennie Parkman. Mentors: Gennie Parkman. Insitution: Weber State University. The principal genes that are responsible for the conversion of benign nevi, or pigmented lesions, to cancerous melanomas have been discovered and validated. However, we are still identifying the additional genetic alterations that are responsible for the migration and invasion of melanoma cells and ultimately metastasis of these melanomas as that is where treatment needs remain. (Davies, et al., 2002)One gene that has been identified to potentially play a role in the metastasis of melanoma is thymocyte selection associated high mobility group box, or TOX. Based on one study, the levels of TOX gene expression were found to be elevated in melanoma brain metastases versus extracranial metastases (Bierman et al., 2022). These findings have led us to hypothesize that TOX plays a role in the migration and invasion of melanoma into the brain. Preliminary work has been completed to clone our gene of interest, TOX, into an expression vector and transduce mammalian melanoma cells to evaluate their effects on melanoma cell rate growth and migration. These experiments will identify the effect of TOX on the initiation and progression of melanoma cells through in vitro melanoma cell culture and experimentation before potentially progressing to well-validated mouse models of melanoma.

Authors: Ana Paulina Medellin-Alvarez, Jared Almazan, Tursun Turapov, David A. Kircher, Karly A. Stanley, Gennie L. Parkman, MiKaela N. Field, Katie M. Culver, Silvia Coma, Jonathan A. Pachter, Howard Colman, Sheri L. Holmen. Mentors: Sheri Holmen. Insitution: University of Utah. Despite promising results from recent FDA-approved therapies, many advanced melanoma patients develop resistance to both immunotherapy and targeted therapy. A common resistance mechanism to targeted therapy is upregulation of the PI3K/AKT signaling pathway, which has also been shown to promote the development of melanoma brain metastases. Historically, AKT inhibitors have failed in the clinic due to their limited efficacy or intolerable toxicity. Proteomic analysis comparing non-metastatic vs brain metastatic primary tumors in mice revealed focal adhesion kinase (FAK) as an AKT1 specific effector and a potential alternative therapeutic target. FAK is a non-receptor tyrosine kinase that localizes primarily to focal adhesions to regulate cell migration. To determine whether targeting FAK alone or in combination with the RAF/MEK inhibitor avutometinib reduces brain metastases and prolongs survival, we utilized both autochthonous and syngeneic melanoma mouse models. Mice with either subcutaneous tumors or established brain metastases were treated with FAK inhibitor, RAF/MEK inhibitor, or the combination of FAK and RAF/MEK inhibitors. Each cohort was assessed for tumor onset, growth, metastasis, and survival. Our results show that combined RAF/MEK/FAK inhibition significantly delays tumor onset, causes regression of established tumors, prevents the development of brain metastases, promotes the regression of established brain metastases, and prolongs survival. In addition, patient-derived BRAF V600E melanoma xenograft mouse models resistant to the BRAF inhibitor dabrafenib and the MEK inhibitor trametinib, were sensitive to combined RAF/MEK/FAK blockade. The addition of the BRAF inhibitor encorafenib to these models further enhanced the effect on tumor growth. These results support the initiation of a clinical trial evaluating the efficacy of the RAF/MEK inhibitor avutometinib in combination with the FAK inhibitor defactinib in patients with brain metastases from cutaneous melanoma. Additionally, we are assessing non-canonical roles of FAK in modulating the tumor microenvironment to determine whether avutometinib and defactinib also enhance the efficacy of immune checkpoint inhibition in this disease.

Authors: Matthew Stecher, Terungwa Iorkula, Matt A. Peterson. Mentors: Matt A. Peterson. Insitution: Brigham Young University. Tropomyosin Receptor Kinase (TRK) inhibition has become an effective therapeutic approach for treatment of a variety of cancers including soft tissue sarcoma, pilocytic astrocytoma, non-small cell lung cancers, colorectal cancers, glioblastomas, pediatric gliomas, papillary thyroid cancers, and secretory breast carcinoma. Despite the successful use of first-generation TRK inhibitors Larotrectinib and Entrectinib which have shown significant therapeutic response in patients, acquired resistance to these drugs has emerged and is creating serious barriers to maintaining long-term therapeutic efficacy. A common mechanism for acquired resistance is associated with the emergence of mutations. Hence, mutation-induced drug resistance continues to be a major challenge for both first- and second-generation TRK inhibitors. Here, we present the design of novel macrocyclic 3,5-diaminated pyrazolo[1,5-a] pyrimidine ligands with high potential for combating drug-resistant disease.

Authors: Corin Marsh, Acacia Overono. Mentors: Acacia Overono. Insitution: Utah Valley University. A variety of memorization techniques have been shown to enhance the ability to recall important information. While many of these techniques focus on repetition and retrieval of verbal information, the drawing superiority effect (DSE, Wammes et al., 2016) is a phenomenon where drawing leads to better recall compared to re-writing. The present study investigates the extent of these findings by combining the DSE with retrieval practice. This experiment utilized a within-subjects, single-blind design consisting of 32 undergraduate students ranging in age from 18 to 42. Participants were tasked with learning 20 vocabulary terms through a guided slideshow, then studied terms through; reading definitions (verbal restudy), viewing images of the definitions (visual restudy), writing definitions (verbal retrieval), and drawing definitions from memory (DSE with retrieval). After a brief distractor task, participants were tested on their recall of the definitions. Finally, subjects completed a shortened version of the Vividness of Visual Imagery Questionnaire (VVIQ) and a funnel debrief. Preliminary analyses using a repeated measures ANOVA revealed that re-reading was the study method that resulted in the highest recall scores (M = .57) compared to all other conditions (F (3, 93) = 14.9, p < .001, ηp2 = .324). Further — VVIQ ability showed no significant correlation with participant scores for any of the study methods. These results indicate that re-reading was a more effective study strategy for memorizing vocabulary terms with a short delay when compared to viewing images, writing definitions, and drawing definitions from memory. Future work will examine how longer delays and providing feedback influence may modulate the benefits of re-reading, drawing, and retrieval as study strategies. Keywords: drawing superiority effect, retrieval practice, memory, VVIQ

Authors: Michaela Thackeray, Cicilee Petersen, Scott Wiltbank, Emma Newman, Enoch Thackeray. Mentors: Matt Draper. Insitution: Utah Valley University. Abstract: Unlocking Well-Being, Workplace Morale, and Employee Retention: A Tech Company’s Success Story This qualitative study, titled "Unlocking Well-Being, Workplace Morale, and Employee Retention: A Tech Company’s Success Story," explores the experiences of employees and leaders at a Utah-based tech company with an unusually low turnover rate. Motivated by the National Institute of Mental Health's call for workplace well-being focus and exacerbated mental health issues during the COVID-19 pandemic, this research investigates the factors contributing to the company's low turnover and high employee satisfaction. The study employs a series of case studies to delve deeply into individual experiences, utilizing phenomenological methods to uncover and describe the depth and breadth of participants' experiences. The research question guiding the study is, “What experiences inform the mental health and well-being of employees and leaders at this low-turnover company individually and collectively?” Qualitative data collection involved interviews with executives, managers, and employees, facilitated through Microsoft Teams software or on-site at the company campus. The data was transcribed, blocked, and analyzed using Giorgi’s Phenomenological Method, a descriptive qualitative approach, supplemented by Nvivo 12 Plus or similar software for a broader thematic examination across participants. The study aims to provide insights into the workplace dynamics that contribute to employee well-being, satisfaction, and retention in the context of a successful tech company. We will present our findings by poster presentation and are open to presenting orally. Thank you for your consideration!

Authors: Ellery White. Mentors: Yi Yin. Insitution: Utah Valley University. This study will examine the influence that parental political beliefs may have on students’ academic performance. Decades of research have formed empirical links between social- contextual factors and student academic achievement, and this paper will seek to identify whether parental political values play a role in the academic achievement of their children (Lee and Shute 2010; Yamamoto and Holloway 2010). Parental attitudes, behavior, and stylistic approaches to their child’s rearing and education have been extensively studied for how they impact educational outcomes (Pandey and Thapa 2017). This study fulfills the gap in research on the association between parental political beliefs and children’s academic performance. This adds to the academic discourse on the impact parents can have on their children in an academic setting by answering the following questions: How can the political beliefs of parents impact a students’ way of thinking and therefore ability to perform in school? Does the extremity of the parents’ political beliefs make a difference in the outcome of the child’s academic performance? A survey was distributed to a random sample of UVU students which measured their academic performance and the political beliefs of their parents. The statistical program SPSS will be used to conduct a multiple regression analysis to examine the relationship between the independent variable (parents’ political beliefs) and dependent variable (students’ academic performance) to see if there is an association between the two. I hypothesize that students with parents who have strong political beliefs are more likely to be impacted in their academic performance than students with parents who do not have strong political beliefs. Discovering the impact of parental political belief will open several important avenues of inquiry related to educational outcomes of students.

Authors: John Walton, Dallin Durfee. Mentors: Dallin Durfee. Insitution: Utah Valley University. We are developing an inexpensive optical power meter to measure lasers in our lab. In the device the signal from a photodiode is directed through a transimpedance amplifier to provide a voltage proportional to the power of the incident light. We will use an Arduino and a 16 bit ADC to collect the voltage measurement. The Arduino will also control a switch, allowing us to change the gain of the transimpedance amplifier for measurements over a larger range of powers. The Arduino will be programmed with the responsivity curve of the photodiode so that the correct power can be calculated for a given wavelength.

Authors: Genna Howard, Cameron John. Mentors: Cameron John. Insitution: Utah Valley University. In the history of psychology, the concept of soul played a significant role informing philosophy and theory. In the late 1800’s many questioned the concept of soul in favor of measurable concepts that aligned with the scientific method, distinguished from religious classification that were deemed unworthy of pursuit. In the emergence of the science method, the concept of soul faded and the field of psychology experienced soul-loss, becoming soul-less. After nearly 150 years, there is a movement in the field advocating for soul to return to its former status by shedding light back onto the soul. For some, soul is aligned with religious thought. Religions are experiencing a loss of congregants, members engaging in faith transitions, and experiencing soul-loss. This paper will compare and contrast what happened in psychology with the experiences of some in religious organizations while looking at how the emotion of fear plays a part in both organizations. The methodology utilizes historical concepts and experiences shared by the brave souls who are challenging the current beliefs in both fields. In addition, anecdotal, informal information provided by individuals close to the authors is used to emphasize the practical application of this crisis in psychology and religions with the goal of reigniting a passion for soul.

Authors: Kate Coneys, Nick Top, Russ Bailey, Logan Walker, Julia Toomey. Mentors: Russ Bailey. Insitution: Utah Valley University. Background: Past literature has emphasized the importance of practicing culturally competent care and has shown that higher clinician cultural humility, as well as a lower frequency of microaggressions occurring in therapy, have been associated with more desirable therapeutic outcomes in retrospective, cross-sectional studies. However, research has also shown that patient outcomes appear to be unrelated to their therapist's experience level (Erekson et al., 2017), suggesting that more experience may not lead to being a more effective therapist, including more culturally humble. Despite the breadth of research on the significance of cultural competence, less is known about how a therapist's cultural humility changes across treatment with a particular client, or how a therapist’s cultural humility changes over an extended period. The aim of the current study is to examine this concept by analyzing both the relationship between the number of sessions spent working with a given therapist and a client’s rating of the clinician’s cultural humility, as well as the relationship between the average cultural humility rating of the therapist and time. We will also examine how the impact of microaggressions occurring in therapy changes during the course of therapy. Hypotheses: It is hypothesized that the results of this study will show a positive association between the number of sessions with the same therapist and their cultural humility, as rated by the client, as well as a negative association between the number of sessions and the impact of microaggressions reported by the client. Additionally, it is hypothesized that the therapist’s cultural humility rating over the entire course of data collection will not change to a statistically significant degree. Methods: We used archival data collected by Utah Valley University’s Student Health Services Counseling Center from August 2021 – September 2023. This archival dataset consists of 564 clients with at least one cultural humility and microaggression data point. Beginning at the third session, and occurring again every four sessions, clients were asked to rate their clinician’s cultural humility, using The Cultural Humility Scale (Hook et al., 2013), and report the frequency and impact of microaggressions that had occurred during the last four sessions. We will analyze the data using multi-level latent growth modelling. Results: Results will be presented as part of the poster presentation. Discussion: If the results of this study agree with the hypothesis, the implications may be that cultural humility improves over time with specific clients, potentially explained by growth of therapeutic alliance over time. However, it may also imply that a therapist’s cultural humility does not improve over extended time periods, showing that cultural humility appears to be static rather than dynamic. If the results do not agree with the hypothesis, this may imply that a client’s perception of their therapist’s cultural humility is static, but that over extended periods of time a therapist’s cultural humility can improve, implying that it is trainable. Sources: Erekson, D.M., Janis, R., Bailey, R.J., Cattani, K., Pedersen, T.R. (2017). A longitudinal investigation of the impact of psychotherapist training: Does training improve client outcomes? Journal of Counseling Psychology, 64(5), 514-524. https://doi.org/10.1037/cou0000252 Hook, J.N., Davis, D.E., Owen, J., Worthington, E.L., Utsey, S.O. (2013). Cultural humility: measuring openness to culturally diverse clients. Journal of Counseling Psychology, 60(3), 353-366. https://doi.org/10.1037/a0032595

Authors: Lindsay Beekman. Mentors: Olga Kopp. Insitution: Utah Valley University. This research probes the primary historical newspapers of early 1900s Utah, providing insights into the cultural atmosphere of the time and the widespread support for the Eugenics movement throughout the state. Our findings indicate that out of 72 distinct newspaper articles discussing Eugenics in Utah between 1908 and 1938, 62 conveyed a positive perspective, 9 remained neutral, and only 1 expressed opposition. Notably, from 1947 to 1996, leaders of The Church of Jesus Christ of Latter-Day Saints administered the ‘Indian Student Placement Program’ or ‘Lamanite Placement Program,’ aligning with many Eugenics principles. Utah, like many places worldwide, embraced Eugenics with open arms.

Authors: Greg Avalos. Mentors: Sierra Warner. Insitution: Southern Utah University. The primary objective of this study is to explore caffeine's impact on explicit memory, particularly during the early morning when cognitive performance tends to be at its lowest. We aim to investigate how caffeine modulates attention, memory recall, and consolidation, shedding light on the less-understood psychological effects of caffeine in a real-world context. Participants will be assigned either the caffeine condition or the noncaffeine condition and will be tested on their explicit memory recall using the Craft 21 story recall test. Expected results are that those in the caffeine condition will score higher on the Craft 21 story recall test compared to those who didn't drink caffeine before the study.

Authors: Megara Jensen, Matthew Hatch, Brock Hughes, Madeline Gillies, Spencer Myres, Maddie Blackham, Autumn Gould, Kate Leigh, Nate Lieshman. Mentors: Rebecca A. Lundwall. Insitution: Brigham Young University. Parenting is stressful. Of all children with special needs, parents of children on the autism spectrum report spending the most time at educational, medical, psychological, and behavioral appointments. They also have significant financial expenses. Therefore, we set out to determine if parents on the autism spectrum are more stressed than parents of neurotypical children. We recruited participants using fliers and posts on social media autism support groups and on bulletin boards across campus. The parents (all mothers) had children between 8- and 32-months-old. For this analysis, we divided families into the ASD-sib group if the toddler had an older sibling with autism and the neurotypical group if they had no first-, second-, or third-degree relatives with autism. We asked them to complete surveys using Qualtrics regarding Family Quality of Life, Parenting Daily Hassles, and Parenting Stress measures. Twenty-three parents completed all surveys (n = 7 in the ASD group and n = 16 in the neurotypical group). We performed an ANCOVA with Parenting Stress as the outcome variable, presence of ASD in the family as the grouping variable, and Daily Hassles and Family Quality of Life as predictors. Age was entered as a control variable. We found a trend for the significance for Family Quality of Life predicting Parent Stress (F [17, 23] = 3.80, p = .083, eta2 = .17). No other variables showed a trend for significance. This suggests that parents of children on the autism spectrum are indeed more stressed. Although we need to collect more data, this motivates us in a larger study we are conducting regarding the effects of mindfulness on parental stress levels.

Authors: Heidi Blaylock, Carter Leuba, Eddie Hill, Christina Aguilar, Carla Cox, Aiden Hill. Mentors: Eddie Hill. Insitution: Weber State University. Type 1 diabetes (T1D) is a chronic disease that influences all health aspects. The self-determination theory (SDT) suggests that three psychological needs of competence, autonomy, and relatedness are necessary for motivation to engage in healthy behaviors. Through medical specialty camps, these needs can be met by educating campers on how to manage T1D and realize they are not alone. The volunteer-based, five-day, inaugural REACH teen/tween camp for youth with T1D was held at WSU. Camp activities were engineered around the three basic needs described by the SDT. These needs were promoted by physical and educational activities, and meeting friends. A pre and post evaluation was given. Two measures were significant with autonomy being the greatest, (M=3.93, SD= .75) to post test (M=4.49, SD= .56), with t(25) = -6.258, p= <.001), effect size r = 1.2 Blood glucose levels were collected through an online platform that allowed staff to monitor campers’ levels. The week's average blood glucose level was 152 mg/dL. This study explored the use of SDT to examine the effectiveness of a diabetes camp for youth and hopefully result in better physical and emotional health thus mitigating the risk of complications.

Authors: Brooklyn Matthews, Andrew Rowley, Michael Salas, Shaun Kader. Mentors: Michele Culumber, Daniel Clark. Insitution: Weber State University. Paucilactobacillus wasatchensis causes significant product loss in the dairy industry by causing gas build-up in packaging, and slit-crack defects in the cheese. By invitation, we tested a cheese manufacturing plant in northern Utah for Plb. wasatchensis in their creamery based on reports of puffed packages of aged cheese. We were allowed to swab several locations in the production area where contamination with Plb. wasatchensis was possible. Swabbed areas included the areas exposed to milk before and after pasteurization, floor drains, the cheese vat, curd cutters, cheddaring table, and packaging environment. Samples of raw milk, fresh curds, and gassy cheese were also collected. Swabs taken from surface samples were vortexed in 1 mL sterile saline solution. The solution was diluted and plated on deMan, Rogosa, and Sharpe agar with 1% ribose (MRS+R) agar. All agar plates were incubated anaerobically for 1 week. Enrichment cultures for Pbl. wasatchensis were also made by inoculating 0.1 mL of the saline rinse from each swab in MRS+R broth, and incubated for 2 days. After growth, were diluted and plated on MRS+R agar. After 1 week of incubation, we selected small (~1 mm), slow-growing colonies that were morphologically similar to Pbl. wasatchensis. The 16S rRNA gene and N-acetyltrasferase gene were amplified using PCR primers specific for Pbl. wasatchensis. Positive Pbl. wasatchensis isolates were found on the handle of the cheese cutter and in the final cheese product showing a gas defect. Our finding suggests that Pbl. wasatchensis is a potential contaminant in the creamery, and though only found in one location, may exist throughout the facility at unculturable levels. Further studies will use molecular techniques to identify Pbl. wasatchensis throughout the facility to identify bacterial DNA instead of live cells, which may more accurately represent the presence of this fastidious organism.

Authors: Amanda Lee, Alex Phillips, Bonnie Baxter, Amy Schmid. Mentors: Bonnie Baxter. Insitution: Westminster College. The Great Salt Lake is home to a massive variety of microorganisms and insights into these populations will aid in understanding the ecosystem. The Union Pacific Railroad’s causeway has divided the lake into two distinct sections and over time caused the North Arm to increase in salinity over the South Arm and gain its hallmark pink color due to restricted freshwater input. Terminal rivers flow into the South Arm while the North Arm’s water evaporates, leaving behind accumulating salty brine, and the two very different waters have nurtured two accordingly distinctive ecosystems. Breaches periodically opened in the causeway allow the waters to mix and provide opportunity for the microbial communities of the two arms to intermingle. In a collaboration between the Great Salt Lake Institute at Westminster University and the Schmid Lab at Duke University, this project focuses on procuring a picture of the North Arm’s microbial diversity and the extent that it adapts to new environments over the four seasons, as well as what happens to the microbes and their nutrient exchange at that breach mixing point. Halophilic archaea and algae comprise the majority of biodiversity in the lake, and we hypothesize that those taxonomic compositions of the North Arm are stable over the different seasons, due to their extremophilic nature. Every season, we have collected water samples from the causeway, breach, and Spiral Jetty to grow and isolate bacterial colonies for DNA extraction and imaging. We also collect biofilm on filters to analyze RNA gene expression and metabolite usage as the environment changes. Over nearly two years of sampling, the hypothesis has been supported and we are seeing stability in community compositions in comparison to the less-salty South Arm. We are still interpreting results from the mixed breach samples, but thus far the microbes appear to be similar to those in the North Arm.

Authors: Brandon Rojas. Mentors: Kristen Arnold. Insitution: Weber State University. The Mountain Haven building in Salt Lake City, a mixed-use facility, offers a wide range of services, including housing, childcare, and healthcare. The Single Parent Project, a non-profit organization, is working to provide suitable housing solutions for single parents and their children. The primary challenge is creating a secure, private, and healthy living environment that supports the emotional, financial, and mental growth of single parents and their children. To address this challenge, the Mountain Haven building must be designed to meet the unique needs of single-parent families. This includes providing access to quality childcare, flexible work arrangements, and efficient household management solutions. It is also important to foster a sense of community and neighborliness and to ensure that single parents have access to affordable and safe housing in a safe neighborhood (Johnson, 1986). Additionally, the arrangement of the home should be flexible to accommodate both work and childcare responsibilities. The design of the Mountain Haven building should also consider the latest research on daycare centers, healthcare facilities, and interior design. This includes using materials that are free of harmful substances, creating spaces that promote learning and nurture children's development (Monoko, Mukurunge, Bhila, 2021), and using light and color to create a healing environment for parents and children to reduce stress and strengthen the mood, mind, and body. (Khaleghimoghaddam, 2023) By incorporating these principles into its design, the Mountain Haven building can provide single parents and their children with a safe, supportive, and nurturing environment in which to thrive.

Authors: Kennedy Parker. Mentors: Melinda Russell Stamp. Insitution: Weber State University. Role-playing is considered one of the most effective teaching strategies for training undergraduate clinical psychology students because it fosters active learning in a supportive and safe environment (Gibbs, 2019; Osborn & Costas, 2013). The literature is replete with studies that document the benefits of role-playing for clinical training. These benefits include the development of active-listening, perspective-taking, empathy, critical thinking, communication, collaboration, problem-solving, and increased enjoyment of course material (Dawood, 2013; Gibbs, 2019; Grose-Fifer, 2017). A challenge associated with classroom role-plays involves student reluctance to participate due to feelings of self-consciousness and anxiety associated with being negatively evaluated (Mallonee, 2018). There is limited research on the effectiveness of different role-play modalities which may include classmates role-playing with one another; as well as actors and virtual avatars role-playing with students. There have been mixed findings in the literature regarding whether students are more comfortable disclosing to virtual avatars or to actors in face-to-face encounters (Rogers et al., 2022; Roster & Chen, 2016). To date, there are no research studies comparing student perceptions of role-plays with virtual avatars to face-to-face interactions with actors in undergraduate psychology classes. For the current study, 60 undergraduate Psychology students were randomly assigned to either role-play with an avatar or an actor. Following the 25-minute role-plays, students completed surveys assessing perceived utility value, self-efficacy, level of anxiety, engagement, and future interest in role-playing. This preliminary study will compare the effectiveness of avatar and actor role-play modalities which will help educators better structure training for undergraduate students in clinical psychology.

Authors: Brandon Barlow, Ethan Reese, Victoria Vazquez, Abbi Stark, Monica Sanchez, Alex Wilson, Rain Evans, Josef Becker, Amy Chevrier, James Taylor. Mentors: James Taylor. Insitution: Utah Valley University. This study focuses on memory and learning observed in Madasgascar Hissing Cockroaches (MHC). Research on insects can be used to inform our understanding of behavior and cognitive processes and add to our existing knowledge about the studied species. While there is existing literature focused on memory and learning in many insects, cockroaches are often chosen as a model due to their adaptability, resilience, and availability. Little research on MHC currently exists, especially in regard to memory and learning or drug seeking behaviors. This study uses a mixed model design, looking at arm choice percentages and latency to choose, between the control and experimental groups and comparing trials for each subject. In earlier trials, MHC demonstrated behavior that indicated they had learned not to fear light, a naturally aversive stimulus for cockroaches. Building on data collected in earlier trials, we developed a new protocol to evaluate the MHC associative learning behaviors and to test drug-seeking as a motivated behavior. Subjects are individually tested in a T-shaped apparatus in which they have to choose an arm to go into or are timed out. Each arm has a specific reward, either a sucrose and ethanol solution (experimental group) or plain sucrose solution (control group) in the left arm, and plain sucrose solution (experimental group) or water (control group) in the right arm. Each subject is run 2 days (6 trials per day) in these conditions, and then 3 days with the left arm lengthened to test if the subjects will seek their preferred reward even if it requires traveling a greater distance.

Authors: Benjamin Holt. Mentors: York Young. Insitution: Utah Valley University. Our research group aims to create an optical system which generates tunable longwave infrared (LWIR) light as a tool for early detection of disease-indicating molecules in human tissue. This system begins with our newly constructed Nd:YVO4-type laser which generates between five and seven watts of continuous wave optical power in the near IR spectrum. This laser light will be used to drive the subsystem responsible for generating LWIR light: an optical parametric oscillator. In order for wavelength conversion to occur via a non-linear process, the near IR laser power serving as an optical drive must have an intensity on the order of tens of MW/cm2. To achieve such intensities the laser power must be concentrated into high intensity nanosecond length pulses by Q-switching the laser. Our method of Q-switching the Nd:YVO4 laser will be via an acousto-optic modulator in the laser cavity. My work to design the modulator’s kinematic mount, cooling system, and RF driver (as well as characterizing the performance of the modulator for use as a laser Q-switch) is described in this presentation.

Authors: Juan Palencia, Tanner Probst, Clair Yeaman. Mentors: Machiel Van Frankenhuijsen. Insitution: Utah Valley University. Abstract: The ABC conjecture is an unsolved problem in mathematics first formulated by Joseph Oesterlé and David Masser. The conjecture attempts to show an analogy between polynomials and integers. This insight arises from the Stothers-Mason Theorem (also known as Mason’s Theorem). The theorem states that the maximum degree of a polynomial is always equal to or less than the number of distinct roots minus one. The ABC conjecture attempts to bridge this analogy by defining the height as the maximum algebraic number in the sum a + b = c where a, b and c are relatively prime. Masser and Oesterle define the radical as the sum of log p where p divides abc. Mason’s theorem states that the height is less than the radical minus one. It turns out that this is not true for the integers. Thus Masser and Oesterle formulated a conjecture which may be true. Over the past decades, this inequality has been strengthened and reformulated. Shinichi Mochizuki has recently published what he claims to be a proof of the conjecture, but many mathematicians are currently still working to understand it. The aim of our research is to better understand the height and radical in the ABC conjecture. In addition, we intend to explore the possibilities of expanding the Stothers-Mason Theorem. Since the ABC conjecture is concerned with an analogy between commutative rings (integers and polynomials), we are interested in seeing whether Mason’s Theorem for polynomials also holds for non-commutative rings, such as matrix rings and the ring of quaternions. In addition, we intend to explore the connection between the factorization of polynomials and finite Abelian groups.

Authors: Samantha Curnow, Carl Hjelmen. Mentors: Carl Hjelmen. Insitution: Utah Valley University. The genome sizes of insects vary immensely; some insects have only 100 million base pairs, while others reach nearly 18 billion base pairs. Many selective pressures can have an influence on genomes, but we still lack a clear understanding of which selective pressures cause such variation in genome size even within well-studied groups. To determine the influence of environmental factors on this variation, we investigate the relationship between genome size and the average natural environment of 152 Drosophila species. The nature of the relationship between genome size and environmental factors is investigated using phylogenetic models and linear analyses such as phylogenetic generalized least squares analysis. We collected data from peer-reviewed papers and publicly available datasets, such as karyotype.org and WorldClim, to compare the genome size of each species to the temperature, altitude, humidity, etc. of their natural environments using linear phylogenetic models. The distribution of these species was also visualized using geographic and biological analysis packages in R, such as geodata, to extract environmental data of specified regions, and phytools in combination with caper to run phylogenetic comparative analyses.

Authors: Roshan Abraham, Parker Jorgenson. Mentors: Daren Nelson. Insitution: Utah Valley University. Hobble Creek is an essential and valuable stream that the City of Springville and other local entities utilize for their water supply. However, the stream is also vital to the local environment and ecosystem since it one of the main streams that the June Sucker (an endangered endemic fish) spawn in each year. Numerous environmental projects and studies have been done in the lower section of Hobble Creek due to this importance. There are many springs in the region that provide water to the stream but there is a lack of consistent monitoring of the flow throughout the stream to determine how the stream is interacting with groundwater sources. Groundwater models completed by the United States Geological Survey in 2005 for the Utah Lake Watershed suggest locations where groundwater interactions should be either discharging or recharging the local aquifer. However, more recent data has suggested that many of these water interaction maps created by the USGS model are inaccurate. Using a water budget analysis, streamflow in the creek is being measured at 10 sites throughout the Hobble Creek Watershed to determine how the flow in the creek changes as it flows towards Utah Lake. Data gathered from this and previous studies show the zones of discharge and recharge shift seasonally and are not as consistent as the USGS model suggests. Recent data does confirm that Hobble Creek is gaining flow upstream and losing flow downstream, but there are portions of the river that also may be perched (do not interact with the groundwater). Further measurements and continued site investigation will be necessary to help us better manage this vital water resource.

Authors: Feyan Hoffman, Roshan Abraham, Christine Fowles, Abi Conger, Scott Jenson. Mentors: Daren Nelson. Insitution: Utah Valley University. One of the least understood part of our local water budget is the interaction of groundwater into our lakes and streams. Groundwater models completed by the United States Geological Survey in 2005 for the Utah Lake Watershed suggest zones where groundwater interactions should be either discharging or recharging into our local streams, wetlands, and other water bodies. However, more recent data has suggested that many of these water interaction maps created by the model are inaccurate, and we need a better understanding of how groundwater interacts with these water bodies. Using a water budget analysis, groundwater and surface water interactions are measured by collecting discharge measurements using flow meters at different cross sections along the Provo River and its tributaries. A gaining or losing stream may swap depending on seasonal changes, such as a particular water year being dry or wet–a stream that is typically gaining may be recharging the aquifer during a dry year. The USGS model is a good basic representation of interactions between groundwater and surface water. Our data suggests that due to highly variable water levels of Utah Lake and local groundwater levels, some discharge and recharge zones in the Provo River subbasin are likely to change over time. In addition, some sections of the river may be perched and not interact with the groundwater system at all. In order for us to better manage these important resources, further analysis of flow behaviors in the Provo River and its tributaries need to be completed. This study is part of a larger study that includes adding two new permanent flow gauging stations to provide a long term record that will enable water managers to better decipher why the groundwater and surface interactions are not fitting the USGS model.

Authors: Fatoumata N'diaye, Kevin Shurtleff. Mentors: Kevin Shurtleff. Insitution: Utah Valley University. The use of solar energy for water purification purposes has proven to be an efficient tool with promising results which could help tackle the issue of water scarcity in developing regions of the world. The low-cost, solar water purification system built previously will be improved through increasing thermal conduction, enhancement of the water flow uniformity, strengthening of the piping system, increasing airflow inside the water purification system, and thus maximizing the clean water production rate. We used aluminum foil as the condensation surface with silicone caulk as a binding agent between the materials. We used ultra-violet resistant greenhouse plastic film to protect the purifier overall from sunlight damage. We measured the incident solar irradiance, airflow inside the purifier, temperature of the evaporation side, temperature of the condensation side, ambient temperature outside, conductivity of the dirty and clean water, and clean water quality/taste. Finally, we compared this data to previously collected data.

Authors: Euiyoung Kim, Bonnie Anderson. Mentors: Bonnie Anderson. Insitution: Utah Valley University. A thermoacoustic engine is designed to transfer heat energy into sound energy. Heat engines have a hot side (hot heat exchanger) and a cold side (cold heat exchanger) and use part of the heat flowing between them to do work. The engine in this study is shaped like a bottle with copper wire mesh heat exchangers in the neck. A heating element wrapped around the top of the neck supplies heat to the hot side and the base of the bottle is made of aluminum to keep the cold side at room temperature. When the temperature on the hot side reaches a threshold the sound wave is created. The heat goes from the mesh of the hot side into the “stack” (steel wool, in this case) and down to the cold mesh. The motion of air as it picks up and drops off heat along the stack excites the engine into one of its resonant frequencies. Different mesh configurations of the heat exchangers were tested to determine the impact on the acoustic properties of the engine. Principal Component Analysis was used to determine which parameters of the mesh affected the acoustic pressure the most. The outcomes of this research not only contribute to the emerging field of thermoacoustics but also provide valuable knowledge for the development of thermal sensors and electric energy in remote locations.

Authors: Silvi Rouskin, Matthew Allan, Dragui Salazar. Mentors: Merrill Halling. Insitution: Utah Valley University. A 3’ untranslated region (UTR) is a non-coding region between the stop codon and the 3’ end of an mRNA. 3’ UTR can regulate post-transcriptional gene expression by influencing mRNA stability, translation, and localization. They fold into complex structures that contain elements and binding sites that interact with various molecules, including proteins and microRNAs (miRNAs). Despite the recognized importance of 3’ UTRs and their structural features, the vast majority of their structures in humans remain unknown. Indeed, the structures of long RNAs in general have been difficult to solve due to their heterogeneity and to the paucity of known, ground-truth RNA structures for training and validating models. This project aims to circumvent these limitations by characterizing the structures of 3,000 to 4,000 human 3’ UTRs. The project can be simply described as follows. The cDNA is received and prepared for PCR with the needed primers. After the PCR, genetic material is transcribed into RNA where dimethyl sulfate mutational profiling with sequencing (DMS-MapSeq) will be introduced. After this, it is reverse transcribed and prepared for sequencing. The project implements thousands of primers to facilitate the comprehensive identification of genes. The vast dataset of structure profiles will be used to develop an advanced machine learning algorithm to predict first the DMS-MapSeq results and eventually the structure of an RNA solely from its sequence. The preliminary results show that it is possible to determine hundreds, even thousands of 3’ UTR structures using DMS-MapSeq and the creating an accurate image of such structure. These results also contain druggable pockets that can be used in RNA based therapeutics in a near future.

Authors: Tyler O'Loughlin, Tyler Daynes, Erik Prazak. Mentors: Dustin Shipp. Insitution: Utah Valley University. In the realm of photon interactions with surfaces, the reflection and refraction of light depend significantly on the micro and macrostructures of the surfaces. Despite the use of various methods to attenuate light, their effectiveness remains a subject of interest. In this study, we delve into the properties that render super-black coatings, such as Vantablack, exceptionally dark compared to coatings with distinct surface characteristics but significantly altered amplitudes of reflected intensity. Our research focuses on understanding the optical properties of Vantablack; in particular, we investigate the role of the carbon nanotube microstructure in achieving its unique light-absorbing properties. Through a comprehensive analysis of our samples, we have uncovered that the carbon nanotube microstructure of Vantablack contributes to its remarkably low reflective intensity and exceptional absorption across all wavelengths of light. Notably, Vantablack displays such a high optical density that even at Brewster's angle, a reflection is virtually undetectable. This research sheds light on the intriguing properties of Vantablack and has implications for a wide range of applications where light absorption and reflection play a critical role.

Authors: Jaylee Montague, Skylar Belka, Gabe Espinoza, Geoff Zahn, Alma Laney. Mentors: Alma Laney. Insitution: Utah Valley University. In May of 2022, a several unidentified trees on Utah Valley University (UVU) campus property exhibited severe virus-like symptoms including: mosaic patterning; yellowing, curling, deformed leaves; and flaking bark. Due to the presence of unidentified aphids and after a literature search, we began investigating the sample for a +ssRNA virus. Extensive testing and RNA sequencing using the MinION v9.4.1 and the cDNA PCR Barcoding kit revealed that neither a ssRNA nor a dsRNA virus were present in the samples, thus, it was suspected that a DNA virus could be the cause. We utilized Rolling Circle Amplification (RCA) on extracted DNA. Following the RCA, gel electrophoresis revealed a strong amplicon larger than 10 kb, suggesting the presence of a DNA virus. Sequencing of the viral genome from the RCA product using the MinION vs10.4.1 chip showed that the mulberry did contain baculoviruses and large DNA viruses. Unfortunately, though, the MinION did not get results for DNA plant viruses, so we sent samples for RNAseq on the Illumina NovaSeq, with results still pending. This is significant since it is likely that the other symptomatic plants near these trees—including redbud and spirea—were infected with the same virus due to RCA amplification in those samples and the presence of the same potential aphid vector. Mechanical inoculations to several herbaceous hosts were done using infected spirea tissue. Furthermore, to our knowledge this is the first report of a DNA virus infecting spirea and redbud in the United States.

Authors: Kaycie A Melville, Ashley N Egan, Yu Ya Liang. Mentors: Ashley N. Egan. Insitution: Utah Valley University. Phaseolus vulgaris (common bean) and P. lunatus (lima bean) provide protein and nutrition for millions of people across the globe. Native to the New World, these cultivated species present unique histories of multiple and independent domestications in Mesoamerica and the Andes. Brazil is the leading producer globally of these beans. With both wild and cultivated landraces present, Brazil may act as a secondary center of domestication for these two economically significant leguminous crops. Brazil's indigenous populations’ cultivation efforts may include both wild and cultivated germplasm, potentially contributing to the continued domestication of these crops to create novel shapes, sizes, and colors that are grown and hand-selected by the natives in these regions. To investigate Brazil as a secondary domestication center of beans, this research addresses several key questions: Are signals of past and current domestication processes discernible in current Brazilian cultivars of these species? Can we unravel the relationships between these Brazilian crops and their primary centers of domestication? What genetic differences are underpinning domestication? How diverse are Brazilian cultivars? How do they relate to wild and related material and species? This work focuses on 48 accessions of P. lunatus and P. vulgaris from Brazil, including wild material and two accessions from two related species, P. polystachios and P. filiformis from North America. Whole genomes were resequenced using Illumina sequencing and compared against the published genome of Phaseolus vulgaris to identify SNPs in each accession. Whole chloroplast genomes were also assembled and compared. Phylogenetic analyses, network analyses, population structure, and tests for neutral and positively selected mutations were conducted, revealing distinct clades for both P. vulgaris and P. lunatus with clear links to their Andean and Mesoamerican origins. Genetic diversity was assessed both within (cultivated vs wild) and between P. vulgaris and P. lunatus, demonstrating significant variations, not only between species but also within each species, suggesting the influence of diverse selection pressures and local adaptation during the domestication process. McDonald-Kreitman tests analyze and locate possible domestication genes from the library of SNP’s between outgroups (Phaseolus filiformis and Phaseolus polystachios) and between species. Overall, this research advances our understanding of the intricate process of domestication in P. vulgaris and P. lunatus, highlighting potential ongoing domestication in Brazilian cultivars, unraveling their genetic relationships, and shedding light on the complex interplay of genes that underpin domestication and diversity. These findings contribute to the broader understanding of crop evolution and have practical implications for crop improvement and conservation.

Authors: Joshua Stubbs, Annette Lewis, Kendall Holcomb, Barbara Suassuna Schincariol. Mentors: Geoffery Zhan. Insitution: Utah Valley University. Does Greater Phylogenetic Distance Affect Competition Outcomes in Fungal Communities?Annette Lewis, Kendall Holcomb, Bárbara Suassuna Schincariol, Josh Stubbs, Geoffery Zahn PhDFungi play a critical role in decomposition, affecting nutrient cycling at a global scale. Saprotrophic fungi competitively decompose dead organic matter. However, the role of phylogenetic relatedness on interspecific competition in fungal communities has not received much attention. The theory of phylogenetic over-dispersion suggests that species within a community tend to be less related than expected by chance, therefore limiting competition due to functional redundancy. Similarly, Darwin’s naturalization hypothesis suggests that taxonomically distinct invaders might experience reduced competition and resistance. In this study, we chose three different saprotrophic fungal species with varying relatedness: Aspergillus niger and Fusarium keratoplasticum (from the same family), and Pleurotus ostreatus (from a different phylum). These species were chosen based on decomposition abilities and phylogenetic distances. These species were cultured and placed in seven combinations to assess their ability to decompose and compete as individual fungal populations and as combined communities (e.g., A, B, A+B, B+C, and A+B+C). Each species was placed near a sterilized piece of paper such that competition was evaluated by analyzing the paper coverage in Petri dishes over three weeks. Each Petri dish was analyzed individually based on the average percentage of paper covered and, within combined communities, the percentage of paper each species covered. Interactions between each species and the percentage of the paper covered was recorded for further analysis. Assessing paper coverage allows for the observation of any potential competitive inhibition of decomposition. We hypothesize that decomposing and competitive abilities would be the strongest with A. niger. Despite the fast growth rate of A. niger individually, preliminary results suggest that it was outcompeted when paired with other species. This research highlights the potential nuances in fungal community interactions influenced by phylogenetic relationships, shedding light on the principles of phylogenetic overdispersion and Darwin’s naturalization hypothesis.

Authors: Anna Wilkinson, Feyan Hoffman, Roshan Abraham. Mentors: Daren Nelson. Insitution: Utah Valley University. Remote sensing technologies, involving drone and satellite imagery, have recently begun to be used in hydrologic research with various applications. The technology is relatively easy to obtain, is noninvasive, and helps minimize cost, access, and safety concerns related to the study of wetlands. The thermal and multispectral imagery can provide unique datasets that expand understanding into groundwater and surface water interactions. As part of this project, we are exploring remote sensing applications within the hydrology of wetlands surrounding Utah Lake, a large freshwater lake within Utah Valley. The project has involved the collection of thermal and multispectral drone imagery from 2021 to 2023. Thermal imagery collected in these areas has assisted in the determining of hot and cold spring locations and how these springs have shifted due to changes in groundwater levels and lake levels. In addition, this imagery has shown how physical anthropogenic influences have also impacted the location and stream channels associated with these springs. Multispectral imagery is used to understand the health of phreatophytes near the lake during dry and wet years to be used as an indicator for groundwater level changes. Even though much of the data is still preliminary and part of a long-term study these remote sensing technologies are already showing how these groundwater systems adapt to seasonal and anthropogenic changes.

Authors: Cole Statham, Katelyn Thomas. Mentors: Michael C Rotter. Insitution: Utah Valley University. Datura (Solanaceae) is a potent hallucinogenic genus of plants that has varied uses across cultures. Tropane alkaloids in these plants are potent compounds that, depending on dosage, can offer the user a psychoactive experience or cause severe reactions including death. For some, Datura is little more than an ornamental flower or an ideal source to have a psychedelic experience. While for others it is a sacred plant, used on special occasions, and only permitted to be handled by those who have been prepared and trained to do so. An explanation for these cultural differences could be that cultures with taboos surrounding this plant are likely those that have species of Datura with variable levels of tropane alkaloids, while cultures without taboos are likely to have species with consistent levels of tropane alkaloids. Here we focus on six species of Datura using literature reports of tropane alkaloid contents and compare the variability in these phytochemicals to cultural uses of these plants. We predict that societies with cultural taboos will be more likely to be those with species that have higher variability in tropane alkaloids.

Authors: Vy Dao, Devin Taylor, Danielle Taylor. Mentors: Devin Taylor, Danielle Taylor. Insitution: Utah Valley University. Epilepsy is one of the most common neurologic disorders affecting about 60 million people worldwide (Chen et al., Poduri and Lowenstein, 2011). Currently in the field, there are various methods being used for mechanical seizure induction using Drosophila melanogaster. This variability makes it difficult to compare findings from different research studies. We employed various mechanical seizure induction techniques currently used in Drosophila epilepsy research. We focused on both group testing as well as individual testing using different anesthesia parameters. For individual testing, epileptiform activity and locomotor activity were recorded in adult male Drosophila through mechanical seizure induction using a vortex assay at either 24-hours post- or 1-hour post-CO2 anesthesia. We also evaluated the time for 50% of adult drosophila recovery as indicated by a group climbing assay (10 flies per group) following mechanical seizure induction using a vortex assay at either 24-hours post- or 1-hour post-CO2 anesthesia. Finally, for each of the above testing parameters, we not only tested wild-type (WT) flies, but also multiple positive control seizure phenotype flies commonly used in mechanical seizure induction studies using Drosophila melanogaster. We found significant effects in both CO2 induction methods as well as in different genetically induced seizure phenotypes.

Authors: Jacob Prescott. Mentors: Zoe Thompson. Insitution: Utah Valley University. The pro-opiomelanocortin (Pomc) gene encodes POMC, which is differentially processed to produce adrenocorticotropic hormone (ACTH), beta-endorphin, and three melanocyte-stimulating hormones, among other peptides. POMC neurons are principally located in the arcuate nucleus (Arc) of the hypothalamus, where they are essential in the control of food intake, energy expenditure and body weight. Several different mutations in the POMC gene have been shown to cause early-onset obesity and adrenal cortical insufficiency in humans. We are working with a mouse model with a hypothalamic-specific POMC deficiency. These mice exhibit hyperphagia, early-onset obesity, and also seem to be infertile. We are interested in examining potential differences in pubertal development, as well as reproductive function. Specifically, we will examine day of vaginal opening, day of first estrus, and estrus cycling in juvenile female POMC-deficient mice. The estrus cycle has four stages: proestrus, estrus, metestrus and diestrus. Although hormone levels in the blood differ during the four stages, these are difficult and expensive to measure. A less invasive measurement is to take vaginal cell samples each day and examine them under the microscope. Three different types of cells are present in different ratios depending on the stage of the cycle: nucleated epithelial cells, leukocytes, and cornified epithelial cells. We will track estrus cycle changes during pubertal development and after to see if there are differences between wildtype, heterozygous, and homozygous POMC-deficient mice.Learning more about how POMC-deficiency affects reproductive function may help us understand more about the link between obesity and infertility.

Authors: Cora Rasmuson, Carly Biedul, David Herbst. Mentors: Carly Biedul. Insitution: Westminster College. Great Salt Lake (GSL) is a unique ecosystem known for its elevated saline conditions, supporting diverse biological communities. However, GSL's vital habitats for birds and invertebrates are diminishing due to climate variability and water diversions. The keystone species, Ephydra gracilis, known as brine flies, is central to this ecosystem's food web.Microbialite mounds of haloarchaea, algae, and cyanobacteria sustain E. gracilis, but receding shorelines and record-low lake levels intensify salinity, reducing food sources and brine fly survivability. This decline has far-reaching consequences, as E. gracilis accounts for over 50% of the diet for 10 million migratory birds at GSL.Ongoing research is focused on refining methods for monitoring populations of Ephydra species in response to changing salinity and elevation levels. This research is vital for the preservation of the lake's ecosystem and biodiversity.

Authors: Heidi Kipp, Annika Jones, . T. Heath Ogden. Mentors: T. Heath Ogden. Insitution: Utah Valley University. The purpose of this research is to identify and document mayfly instars from a parthenogenetic population of individuals being raised in the lab. Instars are the developmental stages of an arthropod between molts. Mayflies (and other non holometabolous insects) go through incomplete metamorphosis, moving from egg to nymph stages, and then to subimago (unique to mayflies), and imago (or adult). Previous research indicates the number of instars to be between 12-45 depending on the species, and the species we studied is estimated to have around 15. During each instar, mayflies shed their exoskeleton. The difference between each instar is vital to understanding the changes in gene expression and overall life cycle of a species. Instars have subtle differences, and identifying them will help further research into which genes are involved in producing specific morphological characteristics. Defining these structures, such as the forming of eyes or wings, are of particular interest in evolutionary phylogenetics. We raised Centroptilum trianguilfer (Ephemeroptera: Baetidae) in a controlled water tank, collecting and dating egg samples from mature imagos. Water is maintained with standard ion composition with 130-400 conductivity. Once the eggs hatched, we performed daily inspections and measurements using a 40x stereomicroscope microscope and Lumenera Infinity Analyze. We are using basic appearance, size and morphology) to separate instars. We will identify and photograph each instar. Samples from the different instars, subimagos, and adults will be acquired and used to generate transcriptome data. We propose that our research will aid in the investigation of mayfly evolution and their genetic mechanisms of control.

Authors: Akir Rowe, Hali Hutchinson, Kingdom Wanjoku. Mentors: Katrina Twing. Insitution: Weber State University. Polychlorinated biphenyls (PCBs) are toxic chemicals popularly used in electrical manufacturing companies from the 1930s - 1970s. PCBs, colloquially known as ‘forever chemicals’, are known to cause significant health concerns and are notoriously difficult to remove from an environment. PCBs are currently banned in the US; however, they can still be found in the environment due to improper disposal methods. This research aims to better understand ecological networks, genomic novelties, and potential bioremediation of microbes from PCB-contaminated environments. Microcosm cultures were developed to resemble the original conditions of the environmental samples, containing PCB-contaminated mud from Woods Pond, Lenox, Massachusetts, and filtered pond water with three different treatments: aerobic, anaerobic, and anaerobic with the addition of sulfate. These cultures have been growing for five months, showing signs of active microbial metabolism (e.g., rust patches in sediment gas production). The microcosms were used as inoculum for agar containing PCBs, and 30 PCB-tolerant colonies were isolated, with some belonging to known PCB-degrading taxa of Paenibacillus, Clostridium, Rhizobium, Methylversatalis, and Sphingobacteria. Future analyses will be conducted to (a) identify the microbial diversity within the microcosms via 16S rRNA amplicon sequencing, (b) explore the metabolisms within the microcosms, and (c) further characterize the isolated PCB-tolerant bacteria. This data will aid further research to determine if and how these microbes metabolize PCB compounds.