Fine Arts

Authors: Abby Hoyal. Mentors: Kristen Arnold. Insitution: Weber State University. The United States is one of the most prominent locations involved in the exchange of children in human trafficking. There are very few outlets that take in recovered children help them receive the proper aftercare and help to gain an education to integrate them back into society. Research has shown that children learn most efficiently in spaces that are modular and flexible. In research conducted for habilitation centers for children, they discovered, “Planning flexibility and variability comfort children and parents, accessibility and emotionality for children visiting [these] centers.” (Kasper, Ilvitskaya, Petrova, Shulginova, 2019). It has also been found that learning levels are highest in spaces that allow the children to learn from their surroundings rather than by just the instruction alone. “An interior shall lead children to learn concepts from working with materials, rather than by direct instruction. [Interiors] should improve cognitive learning, promote independence, curiosity, decision-making, cooperation, persistence, creativity, and problem-solving.” (Manav, 2016). One of the key elements to properly educating children, as listed previously, is to promote independence. One of the ways that research has shown independence to be achieved through design is proper wayfinding elements should be implemented so that occupants do not have to rely on any other occupant to navigate the space. Researchers examined how different colors, light temperatures, and lighting brightness can provide a natural easiness to wayfinding for occupants. Results found, that “the use of cool colors and high brightness levels help people be spatially oriented.” (Hidayetoglu, Yildirim, Akalin, 2012). The Grove will be an Aftercare center that will provide refuge and educate child survivors of human trafficking in a modular environment that educates through the use of space and materials used, with an encouraging push for independence throughout the space due to proper wayfinding elements.

Authors: Morgan Watson. Mentors: Kristen Arnold. Insitution: Weber State University. In the United States, 1 child in every 26 seconds drops out of high school. Out of the 50 million children in America, that are school age, 15 million are unsupervised after school time. With children being at greatest risk between the hours of 3pm and 6pm, these statistics raise a huge red flag. This project’s purpose is to provide peace of mind to working parents, and success for children throughout their school experience and beyond. Research has proven that afterschool program environments are a successful tool used to positively shape and increase healthy behaviors and practices among children (Huang, 2013). School design can affect student behavior, development, and academic performance (Huang, 2013). A key factor in effective school environments is the appropriate use of color. Careful color application has proven to have positive effects on behavior and cognitive learning (Heliyon, 2022). In addition, children have a better school experience when given autonomy. Autonomy improves intrinsic motivation (Ford, 2016). Research suggests that wherever possible, design for autonomy be implemented as a way to support children’s growth and development (Sorensen, 2013). This can be applied through multi-purpose learning spaces and flexible classroom furnishings. Additional spaces for collaboration and creativity are needed to meet the needs of today’s students and hold their attention. Furthermore, autonomy in the classroom environment is supported by designated areas where children can express themselves and make the space their own. Potential areas for this include cubbies and classroom bulletin boards.

Authors: Madi Hancock. Mentors: Nathan Crane. Insitution: Brigham Young University. Binder Jetting (BJ), a type of additive manufacturing (3D printing), creates parts through a multi-layered process. Particles are bound together using tiny droplets of liquid binder. Binder jetting has advantages over other additive manufacturing methods including relatively low costs, fast build rates, and a variety of possible printable materials. However, porosity defects commonly seen in BJ printed parts limit the technology's usefulness in demanding industries. There are several proposed causes of these porosity defects, including poor powder compaction in printed areas, residual carbon from the binder, and powder rearrangement due to binder droplet impact. The relative importance of these factors is poorly understood. This study will compare observations of porosity in printed and unprinted regions of Stainless Steel 316 BJ samples to better understand the possible modes of porosity defects.

Authors: Lydia Beazer, Trevor Carter, Audrey Christiansen. Mentors: Larry Howell. Insitution: Brigham Young University. Outreach is the process of deliberate engagement with a range of diverse communities. It is a vital adaptation in an increasingly digital world, acting as a vehicle to extend the impact of work done in research labs. Increased exposure can attract and inspire future engineers and lead to new opportunities for research. Previously, BYU’s CMR lab invested in consistent outreach projects and collaborated with prominent social media influencers, developing a seven-step plan to connect the public with academic research. Recently, these strategies were implemented in a new collaboration with influential YouTuber Mark Rober. In preparation for the anticipated increased visibility from this project, the lab organized a team dedicated to establishing a consistent and professional digital presence. For months, this team undertook preliminary steps that included updating outdated files, designing appealing and shareable makerspace content, and expanding the archive of publicly accessible resources. Having laid this groundwork, the lab was able to influence the impact of this high-profile collaboration, resulting in measurable increase in several metrics related to exposure and positive interaction with lab research.

Authors: Tyler Hamm, Jake Numbers, Ryan Ruth, Hunter Pitchford, David Allred, Troy Munro. Mentors: David Allred. Insitution: Brigham Young University. Molten salt reactors (MSRs) are being investigated for use in clean energy to replace the common pressurized-water nuclear reactors currently in the United States. MSRs use high-temperature, low-pressure molten salt coolant to provide safer and more efficient energy production. However, many MSRs salt compounds lack tested thermophysical properties, including thermal conductivity. Our research focuses on experimentally measuring MSR salt thermal conductivities using a modified transient hot-wire technique. We use a needle probe, equipped with a thermocouple and heating wires, immersed in molten salt compounds at temperatures running from 400–700℃. Thus far, we have tested the thermal conductivity of LiCl-NaCl (eutectic and 91%LiCl composition), NaCl-KCl (eutectic), LiCl-KCl (eutectic), LiF-NaF (eutectic), and FLiNaK. These test results indicate higher than predicted thermal conductivities and consequential further investigation into the physical properties of our probe to improve the experimental design and data evaluation. This research and improved experimental method will provide accurate and precise experimental results of MSRs molten salt thermophysical properties to populate the national database used by MSR developers which will help further the possibilities and practicalities of MSR technology.

Authors: Bronwyn Brown. Mentors: Ben Felix. Insitution: Utah Valley University. Architectural Analytique of the Thomas S. Monson Center Building with the Scamozzi Order at University of Utah

Authors: Samuel Weisler, Colton Korpi, Josh Lythgoe. Mentors: Aliki Milioti. Insitution: Utah Valley University. The Venice Project addresses the challenge of blending contemporary buildings into the well-established urban environment of Venice, a city well known for its rich architectural heritage. In a city with canals in the place of streets, where motorboats and gondolas are the main mode of transportation, seasonal flooding at high tide continues to become a greater environmental concern. The research centers on the documentation and preservation of these valued characteristics through an analytical and interpretative research approach. The primary focus is on aspects such as perception, harmony of open and enclosed space, and the seamless integration into the urban and environmental fabric.The unique design constraints were taken head on in creating the one of a kind ‘Ca’Meriggiare’, a luxury hotel that enriches Venice’s heritage. In lieu of fighting against them, the design embraces the environmental challenges posed by the periodic flooding of the city and transforms them into an integral part of the design. For instance, the flooding is harnessed to create a charming, arcaded entrance exclusive to hotel guests arriving by boat, providing a unique entry sequence unphased by rising or falling water levels.The expected result of the research is a successful fusion of a contemporary hotel with the rich historical context of the city. The innovative design allowed the periodic flooding to become an integral part of the hotel that added a charm and uniqueness to the guest experience. Ca’Meriggiare stands as a testament to the harmonious integration of historical preservation with environmental adaption that honors Venice’s heritage. This luxury hotel case study offers a holistic perspective on theoretical and design considerations, emphasizing the importance of integration within the environmental dimensions. Rather than viewed as an isolated instance, it provides an overarching framework for innovation that will apply to the evolution of contemporary architecture.

Authors: Harrison Denning, Spencer Thompson. Mentors: Jeff Hill. Insitution: Brigham Young University. Traditionally, tensegrity structures have been a subject of interest for their architectural beauty and high strength-to-weight advantage. The field of tensegrity research has since grown to include robots and more complex latticed structures. More recently, tensegrity has been used to accurately model many biological systems, such as joints and spines. Part of this modeling has involved trying to better estimate these biological systems utilizing bi-stable and multi-stiffness tensegrity structures. Our research takes a closer look at how to build and optimize bi-stable tensegrity structures with multiple stiffness modes. By optimizing tensegrity geometry or spring-cable connections between rigid members it is possible to significantly change the models’ overall equivalent stiffness between stable modes. Our research delves into how changes in the shape of rigid members create differences in overall structure geometry between stable modes and a change in stiffness between the two modes. We also discuss optimal spring cable connections and optimal individual spring constants to further increase stiffness differences between stable positions. Furtherance of this work will involve building larger and more robust models to be used on the body as wearable structures. The application of this research heads towards the development of wearable tensegrity braces with the ability to switch between higher or lower stiffnesses to cater to the needs of the wearer.

Authors: Roya Adham. Mentors: Kristen Arnold. Insitution: Weber State University. Given the frequency and severity of natural disasters and humanitarian crises, around the globe, the current refuge center at Salt Lake City faces critical challenges in providing sufficient support for its demand.Security and efficiency of indoor space are critical matters in designing a refuge center.the absence of confidentiality can lead to information leakage and compromise the security of the building. (Reinten, et al 2017). The room acoustics will impact the creation of positive communication and interaction for individuals who seek solitude and peace inside the building. Oasis Haven promised to serve as a haven to those in need, helping them to nurture and heal from their past traumas. According to recent research, organizing and laying out spaces, and putting directional signage and informational labels to guide individuals who don't know the language will effectively increase their satisfaction level with the space Jamshidi et al. (2020). Accessibility for all individuals in a space is an ultimate goal of the designers and we must ensure cohesive visual language and consistency throughout the entire design.additionally, it studied and proved the effects of color schemes on employees' task performance due to the healing effects of colors Öztürk, Yilmazer, and Ural (2012). color plays a significant role in creating a space that helps refugees feel more at home and provides a sense of belonging to them. certain colors may symbolize peace, warmth, or security in one culture, while they might have different connotations in another Öztürk, Yilmazer, and Ural (2012) we try to incorporate all those principles and get help from other elements such as natural and artificial light to create space that feels like a true home and provide a sense of stability and comfort to refugees during the times of transition and adjustment.

Authors: Jakob G Bates, Matthew B Snyder, Porter Adelman. Mentors: Christopher R Dillon. Insitution: Brigham Young University. High intensity focused ultrasound (HIFU) is a non-invasive medical procedure that uses ultrasound waves to heat up and destroy harmful cells such as tumors. In order to accurately perform this procedure the ultrasound waves must reach and accumulate at a specified target location. This can be difficult to predict because of the way that ultrasound waves reflect, refract, and lose energy within the human body. Tests are run using simulations in order to ensure successful targeting of the ultrasonic transducers. These simulations use data collected from human tissue samples to provide the best results. Unfortunately it is difficult to obtain real human tissue samples from histology labs of hospitals.Our objective is to create a portable measurement device that will allow us to enter histology labs and collect necessary data on site. We propose to design and build an acoustic property measurement device that integrates acoustic transducers with digital calipers. The portability of this measurement device will enable access to a greater number of tissue samples and provide more accurate property measurements. This will lead to better simulations of the HIFU therapy and therefore improve the overall safety and success of the medical procedure.

Authors: Andrea Tyler. Mentors: Kristen Arnold. Insitution: Weber State University. The golden thread that runs through this project is ART. The texture, color, shapes, movement, contrast and emphasis which exists in ART will be carried into the interiors. Through this adaptive reuse project, the interior emphasis will be on the historical architecture and will draw on the textures and details as the backdrop. By tapping into these details, the overall aesthetic will ground the visitor to the experience within the space. The ARTshare Project mission is to uplift artists, gather and unite community, and empower human beings through ART. According to research (Pilegaard, 2023) “exhibitions might be said to produce an atmosphere of its own, neither is detached from the architectural interiors’ existing atmosphere. Indeed, they are dependent on it.” Atmosphere, distance and proximity in museum spaces is how we experience space. Access to light, combined with color, improves alertness and affects the soul and spirit. Combinations of color and natural light by using different types of glass throughout the design will stimulate moods and will be incorporated into this environment. Each loop in the golden thread of this project will represent an area of commercial, hospitality, healthcare and residential. Baniyamin, 2020 says, “Researchers have shown that visual stimulation of nature, natural lighting, artwork, relaxing colours and therapeutic sound can greatly accelerate the therapeutic process”. The art therapy rooms place a high priority on privacy while providing a healing environment with light, biophilia and art. Just like artists rely on nature for inspiration, so will the residential condos. With access to views, air and light, the design will connect the resident with nature. The bistro is the air that the interior breaths, inhaling and exhaling, healing and connecting.

Authors: Mikayla Buckway. Mentors: Kristen Arnold. Insitution: Weber State University. Women represent half of the world’s population, and half of the worlds potential. Many of those women face hardships and issues with nowhere to turn for help. The adaptive reuse design build project was created in response to the non profit organization Haven and their need for a women’s center. The selected area and building are of historic beginnings and are centrally located for the best optimization of the center. The center is needed to have multiple different functions and uses. From safe temporary living spaces to counseling offices the main focus will be to cultivate healing, rejuvenation and learning in the space. Studies show that the built environment we chose to surround ourselves with impacts our wellbeing and can be the catalyst for a hastened or slowed recovery (Sternberg 2020). A range of different design elements will need to be incorporated in the space to ensure the goals of the facility are met. The use of nature, biophilia, color and acoustics in designing the physical environment has been shown to optimize health, wellbeing and performance of tenants. Utilizing these elements in the Haven women’s center will be a crucial part of creating the desired space. Using these positive stimuli will also assist in creating a less institutionalized space and more of a home for healing.

Authors: Tyler Peterson, Anna Jensen, Daniel Orr, Anton E Bowden. Mentors: Anton E Bowden. Insitution: Brigham Young University. Prior to approval for usage, spinal disc replacements undergo rigorous and costly tests to ensure that they will perform as designed once implanted. Wear testing, specifically, is one of the most important tests as any loose foreign particles can cause osteolysis of the surrounding tissue. Due to this, minimizing the wear experienced by a joint replacement is of extreme importance, however, wear testing is often done in latter stages of the design process as a functioning prototype is needed. These tests can be prohibitively expensive and adjustments to the prototype only add to the costs, thus a more accessible test to get an initial estimate of the wear is desirable. There are current methods employed to perform these preliminary test results, including the use of a Tribometer, which tests friction and wear. Benefits of using a tribometer are that it can control environmental factors that parallel the ISO testing standards, however, these devices cost thousands of dollars. In the present work, we designed a cost-effective preliminary testing apparatus that accurately recreates loadings that the implant will experience in flexion and extension, as well as lateral bending. The machine consists of a roller that can be loaded axially by compressing a spring to mimic realistic compressive loading conditions, and a motor that will move the roller to replicate spinal bending. The design incorporates a spring element to ensure that contact between the roller and the implant is preserved. Predictable, repeated motions achieved with this machine can provide early insights into the implant wear modes, which can facilitate iterative design improvements. Such knowledge early in the design process can save costs by reducing the need for major design changes in the late stages of product development.

Authors: Roya Adham. Mentors: Kristen Arnold. Insitution: Weber State University. Given the frequency and severity of natural disasters and humanitarian crises, around the globe, the current refuge center at Salt Lake City faces critical challenges in providing sufficient support for its demand.Security and efficiency of indoor space are critical matters in designing a refuge center.the absence of confidentiality can lead to information leakage and compromise the security of the building. (Reinten, et al 2017). The room acoustics will impact the creation of positive communication and interaction for individuals who seek solitude and peace inside the building. Oasis Haven promised to serve as a haven to those in need, helping them to nurture and heal from their past traumas. According to recent research, organizing and laying out spaces, and putting directional signage and informational labels to guide individuals who don't know the language will effectively increase their satisfaction level with the space Jamshidi et al. (2020). Accessibility for all individuals in a space is an ultimate goal of the designers and we must ensure cohesive visual language and consistency throughout the entire design.additionally, it studied and proved the effects of color schemes on employees' task performance due to the healing effects of colors Öztürk, Yilmazer, and Ural (2012). color plays a significant role in creating a space that helps refugees feel more at home and provides a sense of belonging to them. certain colors may symbolize peace, warmth, or security in one culture, while they might have different connotations in another Öztürk, Yilmazer, and Ural (2012) we try to incorporate all those principles and get help from other elements such as natural and artificial light to create space that feels like a true home and provide a sense of stability and comfort to refugees during the times of transition and adjustment.

Authors: Aksel Anderson, Lindsay Rupp, Anna Busatto, Rob MacLeod. Mentors: Rob MacLeod. Insitution: University of Utah. Cardiovascular disease is the leading cause of death globally, and one of the most impactful subsets is coronary artery disease (CAD). CAD occurs when an obstruction(s) in the arteries fails to supply the heart with sufficient blood flow, ultimately resulting in tissue death. Understanding the geometric structure of the heart’s vasculature can provide insight into the development of CAD. However, previous research has only captured vasculature geometries for the main coronary branches, neglecting the downstream vasculature. Therefore, capturing the downstream vasculature would offer researchers a more comprehensive model to study CAD. Our study developed a method to efficiently obtain subject-specific, comprehensive vasculature geometries. First, we obtained five computed tomography (CT) scans of explanted porcine hearts with the coronaries highlighted via a contrast agent. From these CT images, we developed a novel method to efficiently capture the vasculature geometry of each subject. Once we obtained the final geometries, we computed two metrics to determine the extent of the captured vasculature: (1) the number of vessel segments and (2) the smallest vessel radius. We obtained an average vessel segment count of approximately 169 +/- 63 vessels and a smallest vessel radius of approximately 0.44 +/- 0.15 mm. We were able to successfully capture vessels over 85% smaller than the largest porcine coronary artery with a radius of approximately 3.5 mm. Our methodology will help researchers and clinicians obtain comprehensive vascular geometries to enhance the study and treatment of CAD.

Authors: Korina Ziegler, Aaron McKenzie, Wesley Ziegler, Spencer Maxwell, Bryson Carrier, Charli Aguilar, Alexandra Routsis, Talon Thornton, Jae Bovell, Setareh Star Zarei, Devin Green, Amanda Hawkes, Jeffrey C Cowley, Merrill Funk, James Navalta, Marcus M Lawrence. Mentors: Marcus Lawrence. Insitution: Southern Utah University. ACSM has again determined that resistance training (RT) and outdoor activities are two of the top ten worldwide fitness trends for 2023. We previously found that RT outdoors had a significantly lower perception of effort (RPE) compared to indoor RT, despite no physiological differences in heart rate (HR) and energy expenditure (EE). However, no study has examined other feelings during RT in indoor or outdoor settings. PURPOSE: To determine how indoor or outdoor environments effect perceptions of fatigue and physical activity enjoyment following RT in recreationally resistance trained adults. METHODS: Twenty-three adult participants (n=10 female, n=13 male) completed this study. The Visual Analog Scale Fatigue (VAS-F) measured perceived fatigue and the Physical Activity Enjoyment Scale – Short Version (PACES-S) measured PA enjoyment, and both were measured at baseline and then immediately following an acute session of indoor or outdoor RT. HR was obtained from a chest strap (Polar H10) and EE from a Portable Metabolic Cart (COSMED K5). Randomly in indoor and outdoor settings, participants completed 4 supersets of the reverse lunge and shoulder press exercises using dumbbells at a light (2 sets) and moderately heavy (2 sets) intensity with 1 superset of 6 repetitions per exercise and 1 min rest between supersets. A paired T-test (for HR & EE comparisons) or one-way repeated measures ANOVA with Sidak post-hoc test (for VAS-F & PACES-S comparisons) were used to determine differences (p<0.05). RESULTS: No significant differences were observed between indoor and outdoor RT for the physiological variables of average HR (129.4±17.2 and 127.75±23.3 bpm, respectively, p=0.66) and EE (30.6±11.5 and 28.3±9.9 kcals, respectively, p=0.06). Perceived fatigue significantly (p<0.0001) increased from baseline (1.13±0.94 arbitrary units, AU’s) following indoor (4.54±1.91 AU’s) and outdoor (3.99±1.54 AU’s) RT, but no environmental differences (p=0.36) were observed. PA enjoyment was not significantly (p range: 0.27-0.93) different between baseline (18.73±1.83 AU’s) and following indoor (18.18±1.99 AU’s) or outdoor (18.36±1.99 AU’s) RT. CONCLUSION: In recreationally resistance trained adults, moderately heavy superset RT in indoor or outdoor settings does not alter perceived fatigue or physical activity enjoyment.

Authors: Gabe Matthews, Easton Eddie. Mentors: Jennifer Meyer. Insitution: Utah Tech University. Diabetes is a prevalent chronic health condition associated with significant complications, including diabetic kidney disease. The accumulation of elevated glucose levels in cells triggers an upregulation of NADPH Oxidase (NOX) expression, contributing to diabetic kidney disease. NOX activation results in an increased production of reactive oxygen species (ROS), inducing oxidative stress and cellular proliferation. Pterostilbene, recognized for its natural antioxidant properties, has demonstrated efficacy in reducing oxidative stress across various cell types. This study focuses on elucidating the NOX pathways in endothelial cells exposed to hyperglycemic conditions and assessing the extent of oxidative stress reduction with the introduction of pterostilbene. To quantify cellular oxidative stress, we will employ an Amplex Red assay to measure superoxide and hydrogen peroxide levels within the cells. Quantitative polymerase chain reaction (qPCR) will also be utilized to assess NOX protein gene expression at the mRNA level. To complement these methods, a western blot analysis is conducted to quantify NOX protein concentrations under distinct environmental conditions. Our research aims to shed light on the potential of pterostilbene as a therapeutic agent in mitigating oxidative stress associated with hyperglycemic conditions in endothelial cells.

Authors: Michaela Lovejoy, Rylee Udom, Lindsey Fry, Hailey Roubidoux, Makayla Dunbar, Kali Hunt, Mandy Gibbs, Lisa Welch. Mentors: Lisa Welch. Insitution: Utah Tech University. Traditional dental radiography education necessitates the exposure of both clinician and patients to radiation to build clinician skill. Virtual reality (VR) may enable practitioners to learn valuable hands on skills without the use of expensive equipment and without patient or operator exposure to radiation. The purpose of the research is to determine if access to practice in virtual reality (VR) using a specifically designed application, builds skill competency in dental radiology compared with traditional practice methods. A convenience sample of 24 first year dental hygiene students at Utah Tech University were recruited and randomly assigned to the test and control groups. Test group participants were given access to a VR headset with a dental radiology skill practice application installed. Control group participants were given access to traditional dental radiography skill practice methods. To encourage participation, an incentive of a $10 Amazon gift card upon completion of the study was offered. Data will be collected via skill competency evaluation at two-months, mid-term grade scores and final grade scores from the participants’ dental radiology laboratory course. To increase internal validity and reliability, investigators responsible for skill competency evaluation will be blinded and undergo both intra and inter-rater reliability testing to 95% agreement. Upon completion of the Fall semester 2023, data will be analyzed using ANOVA repeated measures between factors accounting for the two independent variables of traditional practice and practice in VR and the dependent variables of competency assessment scores, mid-term and final clinical course grades. Alpha will be set at 0.05. Should it be determined that practice in VR contributes to student learning, VR may enable students to practice additional health science skills in a safe, low stakes environment; thus, increasing student experiential learning and contributing to increased student and patient safety.

Authors: Marcus M Lawrence, Merrill Funk, Jeffrey C Cowley, Amanda Hawkes, Aaron McKenzie, Alexandra Routsis, Wesley Ziegler, Talon Thornton, Spencer Maxwell, Korina Ziegler, James Navalta. Mentors: Marcus Lawrence. Insitution: Southern Utah University. American College of Sports Medicine has again found that wearable technology and resistance training (RT) are two of the top 5 fitness trends in 2023. Our lab recently found that the bicep-worn Polar Verity device was valid and reliable for measuring average and maximal heart rate (HR) during light intensity circuit RT. However, no study has examined other bicep worn devices during RT while also examining heavier intensities. PURPOSE: To determine the concurrent validity of identical Polar OH1 (x2) and Verity Sense (x2) bicep-worn devices in recording average and maximal HR following moderately heavy RT. METHODS: Twenty-one adult participants completed this study (n=10 female, n=11 male; age: 26.1±9.2 yrs; height: 171.3±9.4 cm; mass: 71.4±18.2 kg; RT experience: 5.7±4.9 yrs). The four bicep devices (Polar OH1 x2 and Polar Verity Sense x2) were worn along with the Polar H10 chest strap, criterion for HR. Participants completed 8 supersets of the reverse lunge and shoulder press exercises using dumbbells at a light (4 sets) and moderately heavy (4 sets) intensity with 1 superset of 6 repetitions per exercise (12 repetitions per superset) and 1 min rest between supersets. Data was analyzed for validity (Mean Absolute Percent Error [MAPE] and Lin’s Concordance Coefficient [CCC]), with predetermined thresholds of MAPE<10% and CCC>0.70. Paired t-tests were used to determine differences (p<0.05). RESULTS: For average or maximal HR, neither the Polar Verity Sense 1 (127.2±17.8 or 151.5±16.7bpm) or 2 (125.7±18.8 or 147.9±18.9bpm) or the Polar OH1 1 (128.7±18.5 or 154.5±18.1bpm) or 2 (129.5±18.2 or 156.4±17.4bpm) were significantly (p range: 0.14-0.97) different than the criterion (128.6±19.2 or 149.3±18.0bpm). However, the Polar Verity 1 and 2 were not considered valid for average HR (MAPE range:16.17-17.57%; CCC range: 0.07-0.13) or maximal HR (MAPE range: 11.60-13.33%; CCC range: 0.02-0.29). The Polar OH1 1 and 2 devices were not considered valid, either, for average HR (MAPE range: 17.22-17.25%; CCC range: 0.08-0.09) or maximal HR (MAPE range: 13.24-13.92%; CCC range: .024-0.27). CONCLUSION: Despite our lab previously finding the Polar Verity as valid for HR measurements during light intensity RT, the current bicep-worn devices should not be utilized during heavier intensity RT for accurate HR measurements. Individuals resistance training and utilizing bicep-worn devices for heart rate should use them cautiously.

Authors: Anthony Cornwall, Jake Rhea, Travis Ficklin. Mentors: Travis Ficklin. Insitution: Utah Tech University. The purpose of this study was to examine the correlation among various barbell tracking methods for both validity and between-method variability. Back squat and snatch lifts were simultaneously tracked using three systems: a corded linear transducer system (Tendo), a laser-based system (Flex), and a reflective marker multi-camera motion capture system (3D). The 3D system was considered a gold standard for validation. Nine repetitions each of barbell back squat and a snatch lifts were executed. Each system simultaneously tracked peak velocity, peak power, mean velocity, mean power, and displacement of the barbell for both lifts. All correlations were tested using Pearson Product Moment, with alpha set a priori at 0.05. Correlations among tracking methods were high for the back squat, and considerably lower for the snatch lift. Correlations for displacement were poor among all methods (-0.63 < r < 0.65). Correlations for velocities and powers were strong in the back squat (0.79 < r < 0.99), but weak-to-moderate for the snatch (-0.31 < r < 0.63). A discussion will be presented of tracking methods and lift mechanics, which can explain some, but not all, disparities among methods.

Authors: Dylan Snook, Anthony Cornwall, Travis Ficklin. Mentors: Travis Ficklin. Insitution: Utah Tech University. The purposes of this study were to (a) experimentally measure the moment of inertia of a regulation baseball and (b) use the same method to measure the new moment of inertia of a modified baseball about orthogonal axes. The moment of inertia (I) of a rolling sphere follows the form I = k*m*r2, where m = ball mass, r = ball radius, and k represents a constant specific to the ball. Because baseballs are multi-layered of varying densities, they were not treated as a solid spheres. The balls were rolled multiple times down a plane inclined at 4.55 degrees and acceleration was derived by video tracking the ball using a camera with optical axis perpendicular to the plane of the ball’s motion. Position and velocity were derived during the rolling acceleration, and the slope of velocity was taken to be the rolling acceleration (acc). This was then used in the equation: k = (g sin(theta)/acc) – 1, where g is the acceleration due to gravity and theta is the angle of inclination of the plane. The variable k was calculated based upon average acceleration over many rolling trials and then used to finally calculate I for each ball. Results for the regulation ball and for the modified balls will be presented for multiple axes.

Authors: Nathan McCoy. Mentors: Jeff Mason. Insitution: Utah State University. Aging-associated changes in motor function often leads to the development of musculoskeletal tremors. In women, the development/severity of tremors is causally related to ovarian failure atmenopause. In the laboratory, mice can serve as an effective model for the development of aging-associated tremors. Based on our previous studies, ovarian somatic tissues transplanted from young mice to old mice significantly decreased the tremor amplitudes and lowered levels ofgliosis in the brains of the older recipient mice, compared to age-matched control mice. The study was carried out using both germ-cell-containing and germ-cell-depleted ovarian tissue. Neurological improvement and overall health were achieved using both types of tissue with similar results indicating that it may be a non-hormonal influence that is responsible for this phenomenon. This study is aimed to identify which properties of ovarian tissue causes these neurological health benefits to occur. Ovarian tissues excrete exosomes, vesicles that can befilled with miRNA which are transported throughout the body. We aim to isolate these exosomes from ovarian tissues using density gradient based centrifugation and have them introduced via injection intraperitoneally into mice to see if the same neurological improvements are achievedas it was done in mice with ovarian somatic tissue transplants. If such improvements are corroborated then ovarian exosomes will be sequenced to identify which miRNA sequences signal the body to undergo these health improvements.

Authors: Wesley Ziegler, Spencer Maxwell, Aaron McKenzie, Talon Thornton, Alexandra Routsis, Korina Ziegler, Jae Bovell, Devin Green, Bryson Carrier, James Navalta, Setareh Star Zarei, Kaye Lavin, Jeffrey C Cowley, Amanda Hawkes, Merrill Funk, Marcus M Lawrence, Charli Aguilar. Mentors: Marcus Lawrence. Insitution: Southern Utah University. Wearable technology and strength training with free weights are two of the top 5 fitness trends worldwide. However, minimal physiological research has been conducted on the two together and none have measured the accuracy of devices measuring repetition counts across exercises. PURPOSE: The purpose of this study was to determine the concurrent validity of four wrist-worn Garmin devices, Instinct (x2), Fenix 6 Pro, and Vivoactive 3, to record repetition counts while performing 4 different exercises during circuit resistance training. METHODS: Twenty participants (n=10 female, n=10 male; age: 23.2  7.7 years) completed this study. Participants completed 4 circuits of 4 exercises (front squat, reverse lunge, push-ups, and shoulder press) using dumbbells at a light intensity with 1 set of 10 repetitions per exercise and 30 seconds rest between exercises and 1-1.5 min rest between circuits. Mean absolute percent error (MAPE, ≤10%) and Lin’s Concordance Coefficient (CCC, ρ≥0.7) were used to validate the device’s repetitions counts in all exercises compared to the criterion reference manual count. Dependent T-tests determined differences (p≤0.05). RESULTS: No devices were considered valid (meeting both the threshold for MAPE and CCC) for measuring repetition counts during front squats (MAPE range: 3.0-18.5% and CCC range: 0.27-0.68, p value range: 0.00-0.94), reverse lunge (MAPE range: 44.5-67.0% and CCC range: 0.19-0.31, p value range: 0.00-0.28), push-ups (MAPE range: 12.5-67.5% and CCC range: 0.10-0.34, p value range: 0.07-0.83), and shoulder press (MAPE range: 18.0-51.0% and CCC range: 0.11-0.43, p value range: 0.00-0.79) exercises. CONCLUSION: The wearable wrist-worn devices were not considered accurate for repetition counts and thus manual counting should be utilized. People who strength train using free weights will need to wait for either improved repetition counting algorithms or increased sensitivity of devices before this measure can be obtained with confidence.

Authors: Kade Robison, David Britt, Elizabeth Vargis. Mentors: David Britt. Insitution: Utah State University. Cytomegalovirus (CMV) is the leading cause of sensorineural hearing loss, the most prevalent form of permanent hearing loss, worldwide. CMV treatment requires long term administration of nucleoside analog antivirals such as ganciclovir (GCV). Although ganciclovir effectively inhibits CMV, it also inhibits neutrophils, an essential component of the immune system, reducing optimal treatment duration. Previous studies have demonstrated that ganciclovir toxicity can be reduced while maintaining effective CMV inhibition by combining subtherapeutic doses of ganciclovir with quercetin, an FDA approved hydrophobic flavonoid with antiviral properties, solubilized with a mitochondria-targeting drug delivery vehicle, Poloxamer 188 (P188). Further efforts have been made to optimize the combinatorial ganciclovir with quercetin encapsulated in P188 (GCV-QP188) treatment by exploring the potential benefits of adding antioxidant vitamins to the GCV-QP188 treatment. One of the pathways by which CMV induces hearing loss is the generation of excess reactive oxygen species, specifically in the mitochondria. Current literature suggests that the toxic effects of the reactive oxygen species produced by CMV in the could be reduced via natural vitamin antioxidant treatments. Ascorbic acid, also known as vitamin C, was the first antioxidant vitamin investigated due to its synergistic antiviral properties when paired with quercetin to treat SARS-CoV-2. Yet, the addition of ascorbic acid into the combinatorial treatment was more toxic than the existing GCV-QP188 treatment. Current efforts are concentrated on assessing the effect of selectively delivering hydrophobic antioxidants to the mitochondria of CMV infected mouse fibroblast cells as targeted antioxidant delivery will require lower antioxidant concentrations, reducing associated toxicity. The addition of hydrophobic antioxidants retinol and alpha-tocopherol, vitamins A and E respectively, delivered via mitochondria-targeting P188 to the existing GCV-QP188 treatment is being investigated to determine if it will significantly improve GCV-QP188 treatment efficacy.

Authors: Anya Weglarz. Mentors: Melissa Watt. Insitution: University of Utah. AbstractBackgroundWomen living with HIV (WLHIV) commonly experience internalized HIV stigma, which refers to how they feel about themselves as a person living with HIV. Internalized stigma interferes with HIV care seeking behavior and may be particularly heightened during the pregnancy and postpartum periods. This thesis aimed to describe internalized HIV stigma among WLHIV giving birth, identify factors associated with internalized HIV stigma, and examine qualitatively the impacts of internalized HIV stigma on the childbirth experience.MethodsPostpartum WLHIV (n=103) were enrolled in the study between March and July 2022 at six clinics in the Kilimanjaro Region, Tanzania. Participants completed a survey within 48 hours after birth, prior to being discharged. The survey included a 13-item measure of HIV-related shame, which assessed levels of internalized HIV stigma (Range: 0-52). Univariable and multivariable regression models examined factors associated with internalized HIV stigma. Qualitative in-depth interviews were conducted with pregnant WLHIV (n=12) and postpartum WLHIV (n=12). Thematic analysis, including memo writing, coding, and synthesis, was employed to analyze the qualitative data.ResultsThe survey sample had a mean age of 29.1 (SD = 5.7), and 52% were diagnosed with HIV during the current pregnancy. Nearly all participants (98%) endorsed at least one item reflecting internalized HIV stigma, with an average endorsement of 9 items (IQR = 6). The most commonly endorsed items were: “I hide my HIV status from others” (87%), “When others find out I have HIV, I expect them to reject me” (78%), and “When I tell others I have HIV, I expect them to think less of me” (75%). In the univariable model, internalized stigma was associated with two demographic characteristics: being Muslim vs. Christian (ß = 7.123; 95%CI: 1.435, 12.811), and being in the poorest/middle national wealth quintiles (ß = 5.266; 95%CI: -0.437, 10.969). Internalized stigma was associated with two birth characteristics: having first birth vs. having had previous births (ß = 4.742; 95%CI: -0.609, 10.093), and attending less than four antenatal care appointments (ß = 5.113; 95%CI: -0.573, 10.798). Internalized stigma was associated with two HIV experiences: being diagnosed with HIV during the current pregnancy vs. diagnosis in a prior pregnancy (ß = 5.969; 95%CI: -1.196, 10.742), and reporting experiences of HIV stigma in the health system (ß = 0.582; 95%CI: 0.134, 1.030). In the final multivariable model, internalized stigma was significantly associated with being Muslim vs. Christian (ß = 6.80; 95%CI: 1.51, 12.09), attending less than four antenatal care appointments (ß = 5.30; 95%CI: 0.04, 10.55), and reporting experiences of HIV stigma in the health system (ß = 0.69; 95%CI: 0.27, 1.12). Qualitative discussions revealed three key themes regarding the impact of internalized HIV stigma on the childbirth experience: reluctance to disclose HIV status, suboptimal adherence to care, and the influence on social support networks.ConclusionWLHIV giving birth in this sample experience high rates of internalized HIV stigma. This stigma was significantly associated with being Muslim, as opposed to being Christian, attending less than four ANC appointments, and reporting experiences of HIV stigma in the healthcare setting. Other factors that were correlated to higher levels of internalized stigma were socioeconomic status, parity, and timing of HIV diagnosis, all of which can impact access to and engagement in healthcare services during the intrapartum and postpartum periods. Internalized HIV stigma impacts the childbirth experience for WLHIV, making the labor and delivery setting an important site for intervention and support.

Authors: Syrus Miner, Ryland Day, Justin LeClair, Adam Dimaio. Mentors: Randy Klabacka. Insitution: Utah Tech University. Accurate measurement of the knee joint angle is important for diagnosis, treatment, and rehabilitation. With the advancement of wearable technology the measurement of range of motion can now be measured dynamically during exercises. The primary purpose of this research project is to provide a mechanism by which physical therapy outcomes can be improved after knee replacement surgery. This will be accomplished by creating a knee brace attachment that will employ motion detecting sensors to chart the post-op and/or post-injury progression of the movement of the knee joint. It is estimated that approximately 5% of the population over the age of 50 in the United States are currently living with a knee replacement. It has been shown that knee pain has been coupled with depression, low self-esteem, eating disorders, and an overall lower satisfaction in life. Improved knee function has reduced the dissatisfaction experienced in life as daily activities are able to be retained. While physical therapy helps recovery of range of motion for patients, the incentive for patients to complete tasks at home and the lack of standardized data collection may impede patient recovery times. The desired outcome of this research is to create a device that reports the effectiveness of a patient's treatment by using off-the-shelf electronics to accurately measure knee joint range of motion and communicate it to physicians.

Authors: ANNA EDLER, RYAN KUNKLER, CASEY WEBB, JACOB MANNING, MARCUS M LAWRENCE. Mentors: Marcus Lawrence. Insitution: Southern Utah University. Climbing (sport and bouldering) has become a very popular hobby for people all over the globe.Now that climbing is an Olympic sport the need for understanding best training practices through evidence, not anecdotal experience, has grown. Using the International Rock Climbing Research Association (IRCRA) individual grading scale based on route completion difficulty,some research has shown that upper limb strength is important for individuals to progress from recreational/intermediate to more advanced/elite levels. However, many studies use non-sportspecific measurements (i.e., hand dynamometer versus a finger climbing hold) and none have assessed rate of force development (RFD) or lower body strength contributions. PURPOSE: To test the hypothesis that compared to recreational/intermediate climbers advanced climbers would have greater dominant and non-dominant upper-body strength and finger RFD as well as lower body compound strength. METHODS: Nineteen subjects (n=8 female and n=11 male;age: 24.7±7.5 yrs; height: 177.6±7.8 cm; mass: 76.0±14.9 kg; IRCRA Sport Grade: 14.1±6.7; n=10 intermediate, n=9 advanced) completed this study. During a single session, following a standardized 3-5 min. warm-up all participants dominant and non-dominant finger strength andRFD (using a Tindeq dynamometer load cell attached via static rope to a 20mm edge) as well as shoulder strength (using the same Tindeq load cell with a static rope attached to an olympic ring), and lower-body compound strength (isometric mid-thigh pull using G-strengthdynamometer load cell attached to a straight bar with a static rope) were assessed. Three trials were done on each measurement with 1 min. between trials and 3-5 min. between tests. Unpaired t-tests determined differences, p<0.05. RESULTS: Across every measurementadvanced climbers had significantly (p<0.05) higher values for absolute and relative (normalized to body weight, BW) measurements. As absolute and non-dominant results were similar we onlyreport dominant relative results, where appropriate. Indeed, compared to intermediate climbers advanced climbers had significantly higher relative dominant finger RFD (9.9±3.7 vs 20.8±9.4N/s*BW -1 ), finger strength (0.5±0.1 vs 0.7±0.3 kg/BW), shoulder strength (0.7±0.2 vs 0.9±0.2 kg/BW), as well as relative compound strength (1.8±0.4 vs 2.6±0.7 kg/BW, respectively). CONCLUSION: Advanced climbers have larger absolute and relative RFD in their fingers,stronger dominant and non-dominant fingers and shoulders, as well as stronger lower body compound strength. Thus, individuals looking to progress from recreational/intermediate climbing grades to advanced/elite should focus on improving total body absolute and relative strength as well as finger RFD.

Authors: Caroline Nielson, Connor Baird. Mentors: Robert Kagabo. Insitution: Utah Tech University. Opioid Use Disorders Treatment Related Disparities in Patients with Psychiatric IllnessAuthors: Caroline Nielson, Connor Baird, Robert Kagabo, PhD, MSW, MPHAbstractBackgroundThis study is a review of peer-reviewed articles of Opioid Use Disorders (OUD) treatment among individuals with psychiatric illness. OUD is a subset of substance use disorders (SUD) that is chronic and a growing public health concern. There were 47,000 opioid-related deaths in 2018 in the US; OUD is responsible globally for 68% of the drug-related deaths. OUD can result from either prescribed or non-prescribed opioid use. Some populations such as individuals with psychiatric illness have high rates of prescription opioids yet face opioid use treatment and research-related disparities. This review study examines OUD treatment and research-related disparities among patients with psychiatric illness with the goal to improve treatment among this vulnerable population.MethodsUsing PubMed and PsycINFO databases, we performed a search of journal articles regarding OUD treatment which were published between 2010 and 2023. We then completed a review of the journal articles using narrative overview guidelines. All articles reviewed were from randomized controlled trial (RCT) studies. Examples of search terms used include OUD treatment and mental health; OUD and psychiatric illness RCT studies; and opioid use treatment and mental health. Studies included were those of OUD treatment among individuals with psychiatric illness and or with a DSM5 or DSM4 diagnosis. Any studies that did not include OUD treatment and, or a DSM diagnosis, or RCT design were excluded. To avoid duplication, only one journal article from a study was included. ResultsThere were 18 journal articles reviewed that met the inclusion criteria. Treatment periods ranged from 8-24 weeks. Generally, studies excluded individuals with diagnoses such as schizophrenia, schizoaffective disorders, or other serious mental health diagnoses. Several studies included participants with a DSM5 or DSM4 diagnosis of OUD, while excluding individuals with other psychiatric or substance use disorders. Most of the studies used pharmacological treatments and only a few studies had combined pharmacological and behavioral treatments. The common medicines in pharmacological treatments were methadone, buprenorphine, and naltrexone.ConclusionOUD treatment and research related disparities exist among individuals with psychiatric illness, yet this is the population who suffer most from OUD. Additional studies regarding OUD treatment among individuals with psychiatric illness are needed to improve OUD treatments and reduce OUD-related morbidity and mortality rates.

Authors: Kylee Fernandez. Mentors: Gennie L Parkman. Insitution: University of Utah. Evaluating the role of LRRC1 in melanoma growth and metastasisThe development of brain metastases is a critical problem in Utah, regardless of the novel therapies created for melanoma. The melanoma mortality rates in Utah are 31% higher than the national average and the melanoma incidences are 80% higher. To lower these incidences, our research is aimed at investigating targets in melanoma that play a crucial role in brain metastasis and can be identified as therapeutic interventions.LRRC1 is a gene known as Leucine Rich Repeat Containing 1. LRRC1 is involved in early-stage adipocytic differentiation. Altered gene and protein expression of LRRC1 is observed in melanoma potentially due to the vital role LRRC1 plays in adipose stem cells that differentiate by adopting the phenotypic, biochemical, and functional properties of more terminally differentiated cells. To evaluate the effect of LRRC1 on melanoma growth and metastasis, we are using an established autochthonous in vitro and in vivo model of melanoma based on the RCAS/TVA avian retroviral system. The results of this study will have tremendous potential to contribute to the understanding of the genetics of this heterogeneous disease.

Authors: Adriana Bibo, Nicole Losurdo, Nichole Link. Mentors: Nichole Link, Cindy Greaves. Insitution: University of Utah. Microcephaly is a neurodevelopmental disorder characterized by occipital frontal circumference (OFC) more than two standard deviations below the mean. It can be accompanied by comorbidities including intellectual disabilities, seizures, and other developmental phenotypes. Microcephaly is attributed to malnutrition and exposure to toxins or infection during pregnancy, but genetic mutations are also a leading cause. To investigate the genetic mechanisms behind microcephaly, our lab collaborates with a physician who has completed whole exome sequencing for two hundred microcephaly patients. He identified potentially pathogenic human variants, which we are studying using Drosophila melanogaster as our model organism. We screened for loss of function phenotypes through RNAi knockdown of fly orthologs to determine if these genes are necessary for brain development. In our primary screen, we found that RNAi knockdown of Bx42 in neural stem cells or post-mitotic neurons causes significantly reduced brain lobe volume. In our current study, we are assessing potential causes for reduced brain lobe volume. We seek to determine if RNAi knockdown of Bx42 in neural stem cells or neurons affects cell number, proliferation, or death in developing brains.

Authors: Grace Moore. Mentors: Aaron Olsen. Insitution: Utah State University. AbstractAnimal care technicians for research labs have many responsibilities in order to maintain the integrity of a research project. However, it is difficult to determine what tasks will require most of a technician’s time and effort. Thus, it is proposed that tracking what each individual does during a given shift can allow supervisors and technicians to create an efficient schedule of how they will work and provide a standard for individuals to meet every day. This project consists of tracking in detail what an animal care technician does in a given “shift”, including observation hours, handling and restraining, working in different biosafety levels, cleaning and prepping caging, and changing the caging. This data collection, over several months of work, finds relationships between tasks, the frequencies of being completed, and the time they take. The key tasks of an animal care technician are observing, providing nourishment, maintaining clean environments for both the researchers and the subjects, and maintaining supplies and accurate records. Additionally, many hours are dedicated solely to observing the animals to ensure their health and safety. Moreover, cleaning and prepping of caging or supplies are completed more than half of the time during a given shift. Finally, working with a given species may require more time to complete a task (such as cage changing or cage preparation) compared to another species despite the task being the same. If each technician were to track their tasks and create a profile of personal statistics, they would be able to easily determine how long a task should take. Moreover, a supervisor would be able to determine if a technician is working more efficiently or even falling behind during daily tasks. Overall, these findings could be used to incorporate better time management or planning for institutions to know what more time or resources may need to be devoted to in order to maintain a safe environment, provide exceptional care for the subjects, and follow every protocol.

Authors: Sylvia Lee, Ainsley Parkins, Rodolfo S Probst. Mentors: Rodolfo S Probst. Insitution: University of Utah. Mutualisms between ants and plants are ubiquitous interactions and have been evolving for over 100 million years. Studies have been done on systems wherein the plants provide nutritional resources for ants, but the dynamics of mutualistic interactions in environments where host plants do not provide obvious nutritional rewards continue to remain elusive, raising questions about their co-evolutionary histories. In these circumstances, associated fauna, such as mealybugs and soft scales (Hemiptera: Coccoidea: Pseudococcidae and Coccidae), serve as intermediaries of the association, providing honeydew (enhanced plant sap) as a main food source to their host ants. However, the identity of the insects involved in these associations and their co-evolutionary history with ants is poorly understood. By focusing on samples collected in Costa Rica from 33 colonies of the Neotropical plant-ant genus Myrmelachista (Formicinae: Myrmelachistini), we sequenced DNA barcodes (cytochrome oxidase subunit 1 [CO1]) using Oxford Nanopore Technology and employed Next-Generation Sequencing (NGS) for both the Myrmelachista ants and their associated fauna. We aimed to identify their taxonomy, phylogenetic relationships and identify unique interactions between ants and their associated fauna. We recovered DNA barcodes for eleven Myrmelachista species and seven putative species of mealybugs (n=5) and soft scales (n=2). For the seven putative species of Coccoidea, our DNA barcodes, when compared to available sequences from the NCBI database, recovered an average of 85% match similarity. The closest matches associated our putative species to Old World Coccoidea taxa, reinforcing the need for taxonomic studies of this superfamily for the Neotropical region. Each putative species of soft scales was found uniquely associated with two Myrmelachista species (M. flavoguarea and M. cooperi). For all eleven Myrmelachista species, five were associated with two putative mealybug species. One putative mealybug species was associated with five different Myrmelachista species; one was uniquely associated with M. nigrocotea. Same associations were found between the associated fauna of M. flavocotea and M. lauropacifica; M. haberi and M. RSP027; M. plebecula, M. meganaranja, and M. osa, respectively. These results suggest that there may be unique associations between some Myrmelachista species and their Coccoidea associates, unconstrained by the ant phylogeny. Our findings also provide insights on Coccoidea taxonomy, emphasizing NGS DNA barcoding as a tool to illuminate how ant-plant associations might have evolved.

Authors: Ainsley E Parkins, Sylvia Lee, Elizabeth J Heath, Thomas Petitjean, Kyle Cink, Kyle Kittelberger, Cagan Sekercioglou, Rodolfo S Probst. Mentors: Rodolfo S Probst. Insitution: University of Utah. Biodiversity loss poses a significant threat, requiring accurate species assessment and conservation strategies. Non-invasive and cost-effective techniques should be prioritized, especially when dealing with threatened vertebrate fauna. By focusing on cryptic Eastern European species within the genus Emberiza (Passeriformes: Emberizidae), we designed an affordable molecular pipeline (including DNA primers and bioinformatics) integrating DNA barcoding with Nanopore sequencing to address species boundaries and provide conservation information for avifauna diversity. We conducted non-invasive DNA extractions from individual feathers of 192 specimens collected in the Aras River Riparian Corridor (ARRC), an important ecological hotspot in Turkey. By targeting the cytochrome oxidase subunit I (CO1) region, our goal was to differentiate Emberiza taxa and identify potential cryptic lineages while using cutting-edge next-generation sequencing (NGS) of multiplexed specimens with portable Oxford Nanopore Technologies. Our focal study examined boundaries in Emberiza subspecies (E. shoeniclus shoeniclus and E. shoeniclus caspia) with subtle morphological differences, utilizing DNA barcoding and Nanopore sequencing. We obtained 182 Emberiza DNA barcodes. Clustering analysis recovered a paraphyletic assemblage (n=32) and a clade, the latter recovering majority of the barcodes (n=150), separated by at least 8% genetic difference. In both cases, we observed a mixture of barcodes from the nominal subspecies and Caspian subspecies, suggesting that cryptic morphological characteristics are not reflected in genetic separation. Our results also suggest putative cryptic Emberiza species, highlighting the importance of the ARRC for conserving avian diversity. We demonstrate that our pipeline, integrating real-time Nanopore sequencing with non-invasive DNA barcoding, can be used for conservation initiatives (particularly aiding cryptic species identification). This study expands our knowledge of avian diversity and highlights the potential of NGS DNA barcodes as a powerful tool for aiding conservation efforts amidst biodiversity loss.

Authors: Alexander Romano, Matthew Hesterman, Rachel Hill, Melodie Weller. Mentors: Melodie Weller. Insitution: University of Utah. Sjogren’s Disease (SjD) is an autoimmune disease diagnosed by symptoms of reduced tear and saliva excretion, accumulation of lymphocyte foci in the salivary glands, and the occurrence of antibodies against Ro (SSA), La (SSB), and nuclear proteins. The cause of SjD is unknown, though previous studies have detected the sequence and antigens of Hepatitis Delta Virus (HDV) in the minor salivary gland acinar, ductal, and adipose cells of patients with SjD; without the typical presence of a Hepatitis B coinfection. In this study, murine models were transduced with Adeno-associated virus containing expression cassettes for Luciferase (control), small, large, or a combination of both small and large HDV antigens to evaluate the impact of HDV antigen expression on salivary gland function and SjD autoimmune disease development. After a ten- or four-month period, the models were analyzed. Findings included a significant increase of inflammation for samples expressing both small and large HDV antigens, a significant increase of anti-SSA(La) antibodies in samples expressing the short HDV antigen, and a correlation between increased overall inflammation and decreased overall saliva flow. Performing qPCR methods verified the amount of HDV in the submandibular glands and Illumina sequencing portrayed an increase in glycolysis and beta oxidation metabolism in models with detectible HDV sequence. The models showed significantly increased IgM expression in the HDV exposed murine models, without significant change of other antibodies. Future plans include an ELISA diagnostic assay to verify the antibody levels and further sequencing analysis. With this information we can build a better picture of the direct mechanisms of HDV-mediated changes in salivary gland dysfunction and determine the extent that HDV can inducing systemic SjD symptoms.

Authors: Bradley Lawson, Lukas Keller, Marshall Burrows, Sam Perkins, Emery Wheeler. Mentors: Ronald Sims. Insitution: Utah State University. Subcritical water hydrolysis (SWH) is a developing method of processing organic materials/waste without the use of acids and alkali materials. SWH lyses peptides differently than acid hydrolysis and can produce Maillard products, which have various effects in cell culture. In this study, a batch-mode pressure vessel with a semi-continuous sampling apparatus will be used to optimize temperature and retention time for the hydrolysis of Chlorella vulgaris biomass for the development of serum-free (or low-serum) cell culture media. Hydrolysis will be done at temperatures from 140°C to 300°C and retention times from 5 minutes to 30 minutes. The SWH products will be assessed for their general protein content, lipid content, bioactivity, and carbohydrate content. These efforts will be of use to Upside Foods in its efforts to create sustainable and ethical cultured meat products.

Authors: Jane Damstedt, Kasey Cole. Mentors: Kasey Cole. Insitution: University of Utah. In this project, we are analyzing faunal remains recovered from Utah’s House Mountain Range in the Tubafore cave system to create a paleoecological baseline of life in that location over the past 1000 years. All animals have relatively consistent ecological tolerances and preferences, so comparing the data from the information obtained in this study with contemporary accounts of mammalian ecology in the same area will be useful in evaluating the role of anthropogenic climate change in mammalian ecosystems. In this project, I am identifying the animal remains to the lowest level of taxonomic classification possible to determine what kinds of species richness and environment were present at the time in which the animal was alive. Creating an accurate record of the animals found in this cave assemblage is critical because very little is known about the species richness of the Great Basin area. This data will then be stored and compared to modern zoological data, which came from online databases Actos and Vertnet. The information gathered from this project can be used to inform conservation and sustainability efforts aiming at preserving biodiversity in Central Utah’s Mountain Ranges.

Authors: Ada Cottam Allen, Mikhael T Semaan. Mentors: Mikhael T Semaan. Insitution: University of Utah. Regularly buoyed from thermodynamic equilibrium by complex and fluctuating environments, living systems must continually respond and adapt to external stimuli. Recent tools from stochastic thermodynamics place concrete energetic and entropic bounds on these processes, for systems arbitrarily far from equilibrium both in and out of steady-state conditions, and thus reveal mechanisms by which these systems absorb and dissipate energy to function. Here, we leverage these tools to simulate and calculate the thermodynamic signatures of several models of sodium and potassium ion channels---channels crucial to propagating action potentials in mammalian neurons---as driven by biologically-realistic action potential spiking protocols. Thus uncovering their detailed energetic responses to such complex environmental conditions, these results enable detailed comparison between the thermodynamic functionality of each channel type with driving one would expect in-vivo. When applied to competing models of the same channel, these tools also provide additional criteria for model selection; to this end, we close by calculating expected ionic currents, directly facilitating comparison with dynamically-driven patch-clamp experiments.

Authors: G. Colton Gardner, Lynasi Gapelu, Sohom Mookherjee, Sihem Boudina, J. David Symons. Mentors: J. David Symons. Insitution: University of Utah. With the prevalence of cardiovascular disease increasing world wide and its precise mechanisms remaining elusive, the need for novel treatments is urgent. Autophagy is a conserved process whereby dysregulated intracellular proteins are escorted to the lysosome for recycling. Emerging evidence indicates that autophagy is important in maintaining endothelial cell (EC) proteostasis and function. My laboratory showed that autophagy repression in adult mice ECs produces arterial dysfunction, and indexes of autophagy are repressed in ECs of older mice and humans which display concurrent EC dysfunction. Sequestosome 1 (p62) tethers proteins and enables their entry into the autophagic pathway. The contribution from EC p62 to cardiovascular and metabolic function is unknown and experiments are needed to investigate this. Thus, p62 will be depleted from ECs of adult male and female mice (p62iEC-/-) via tamoxifen regimen, and results will be compared to animals wherein EC p62 is intact (CON). The efficacy and specificity of EC p62 depletion will be evaluated in ECs and vascular smooth muscle by qPCR and immunoblotting. Three adjacent 1 mm thoracic aorta sections will be obtained 14 days after the tamoxifen regimen. The central aorta section will be used to measure intrinsic mechanical stiffness by wire myography, with the other two used to find lumen diameter and intimal medial thickness. These measurements will help create Stress vs. Strain curves and the Elastic Modulus, which will be compared between the groups to show the differences caused by p62 depletion. We expect p62 depletion from ECs to increase intrinsic aortic stiffness.

Authors: Sydney Larsen. Mentors: Joshua Steffen. Insitution: University of Utah. Pollen preserves are crucial to maintaining Apis mellifera’s hive health. This pollen provides bees with a source of protein, amino acids, lipids, and other nutrients that are vital for larvae development (Dharampal et. Al, 2019). In addition to providing these essential nutrients, bee collected pollen also provides the hive with an excellent source of external pollen-borne microorganisms. These microbes provide a variety of benefits including serving as a major dietary requirement for larvae, assisting in biochemical processes such as bee bread fermentation, and assisting in bee resistance to pathogens (Gilliam, 1997). Despite the importance that these organisms play on bee health, there is currently little research on how the microbial composition of bee foraged pollen changes throughout the foraging season in response to abiotic and biotic factors. Rapid advancements in DNA metabarcoding approaches and DNA sequencing technologies have made the assessment of hive level foraging patterns easily accessible. We attempt to leverage the benefits of these new approaches to characterize how microbial composition in pollen provisions changes in relation to shifts in foraging preferences. Here we describe general patterns plant foraging behaviors for two hives across a single foraging season. In addition, we describe the variability of microbial diversity associated with pollen across this same time frame. Our initial description plant and microbial communities present in pollen provisions suggest that pollinator foraging preferences play a significant role in pollen associated microbial communities. This will allow for further research into how the presence of specific beneficial microbes is affected by changes in climate, floral availability, pathogen presence, etc..ReferencesDharampal, P. S., Carlson, C., Currie, C. R., & Steffan, S. A. (2019). Pollen-borne microbes shape bee fitness. Proceedings of the Royal Society B, 286(1904), 20182894.Gilliam, M. (1997). Identification and roles of non-pathogenic microflora associated with honey bees. FEMS Microbiology Letters, 155(1), 1–10. https://doi.org/10.1016/s0378-1097(97)00337-6

Authors: My Nha Quyen Tran, Cindy Greaves. Mentors: Martin Horvath, Cindy Greaves. Insitution: University of Utah. I have been studying the DNA repair enzyme MutY found at the Lost City thermal field 3000 feet deep in the Atlantic Ocean with conditions similar to those that may have shaped the origin of life. With no sunlight and very little oxygen, life at the Lost City is driven by chemical transformations in rocks at extremely high levels of pH and temperature. DNA in all organisms strictly follows base pairing rules in which A pairs with T and G pairs with C. We all think of oxygen as beneficial, but it can cause damage to DNA. Oxidized guanine (OG) violates the base pairing rules and pairs with A. This causes mutations, which can lead to changes in the genetic information. The mutagenic potential of OG was a challenge that had to be solved early in the emergence of DNA-encoded life. The enzyme MutY functions in suppressing mutations and therefore protects us from developing cancer by finding and removing A from OG:A mispairs. In my effort to replicate conditions at the Lost City so as to explore how the DNA repair enzyme MutY evolved to function in this strange environment I need to purify the enzyme. I used DNA cloning to attach the MutY-encoding genes to a soluble bacterial protein called GST that hopefully will make it easier to purify the MutY proteins. Encouragingly, these fusion proteins expressed well in bacteria and appeared to be soluble. I am now exploring different conditions looking for optimal enzyme activity, which I predict to be different from what familiar bacteria can tolerate. Basic science research on how DNA repair enzymes adapted to the strange environment at the Lost City may inform future translational applications to treat and cure cancer and thus ensure a healthy society in Utah.

Authors: William Lee, Rishab Nayak, Cecilia Knapp, Camila Soderholm. Mentors: Ryan Stolley. Insitution: University of Utah. Recent publications have shown pyridine carboxylate functionalized aza-crown ethers coordinate strongly to a number of rare earth elements. Considering their strong coordination, we have modified these crown-ethers with a number of hydrophobic moieties to allow for adsorption to non-polar substrates to enable packed bed isolation of these elements to address the broad use and limited availability of these elements.

Authors: Nathan Jackson, Ryan James, James Crutchfield. Mentors: Mikhael Semaan. Insitution: University of Utah. Symbolic dynamics allows for modeling---and designing for---the effects of imperfectly measuring a time series of data, by partitioning into a finite number of possibilities. The resulting time series of discrete symbols is then made especially amenable to information-theoretic methods for understanding its temporal structure and correlation. In particular, as long as the partitioning scheme is generating, the resulting estimations for entropy rate---the rate at which the process creates information---converge to a measure of chaos in the underlying system, a dynamical invariant.However, the entropy rate is not sensitive to what kind of generating partition: colloquially, an instrument must be at least accurate enough, but can be more fine-grained as desired. In contrast, its breakdown into a piece which affects future measurements (“bound”) and a piece which does not (“ephemeral”) depends quite dramatically on the choice of generating partition. We ask, then: is there a canonical partitioning scheme for which the full suite of information measures relate to dynamical invariants?To tackle this, we simulate the well-known tent and logistic maps, sweeping over all two-boundary partitions, calculating the full information anatomies for each. We find that ephemeral and bound information are extremized by the single-boundary coarsest generating partition, suggesting a canonical role for the simplest “good enough” instrument. The remaining multivariate measures, meanwhile, reveal hitherto-unseen structure in the process of imperfect measurement.

Authors: Sebastian Partida. Mentors: Jamie Spinney. Insitution: Southern Utah University. Lighting levels have an important role in influencing the ambiance and functionality of various land uses. To achieve specified goals while optimizing energy efficiency and sustainability, lighting intensity must be balanced. The purpose of this study was to compare light intensity for different land uses (e.g., residential, commercial, industrial, and recreational). This study measured light intensity (i.e., brightness) for a random sample of different land uses using a digital light meter within Cedar City, UT. GPS coordinates were also acquired in order to map the brightness data. As expected, the results revealed that residential land uses were the darkest, whereas commercial and industrial land uses were the brightest. The findings indicate communities have to maintain a balance between creating a warm and pleasant atmosphere and ensuring appropriate illumination for safety and security purposes. Energy-efficient LED fixtures and smart lighting systems, for example, can provide adjustable and programmable illumination settings that meet both aesthetic and safety requirements.

Authors: Daniel Davis, Paul B Frandsen. Mentors: Paul B. Frandsen. Insitution: Brigham Young University. The nut and acorn weevils of the genus Curculio (Coleoptera: Curculionidae) are a diverse group of beetles with a unique life history. A female weevil uses her rostrum (snout) that is about the length of her body to dig into the flesh of a developing hard-shelled seed and lay her eggs inside where they can safely develop into larvae. After the grown larvae exit their seeds, they spend one or more years burrowed in the soil near host trees. During this time they enter diapause, a state of suspended development to minimize the energy that they expend. Studies indicate that a major purpose of this behavior is to align their adult emergence with masting events (large scale seed production every 2-5 years) of their host trees. Between, and even within, Curculio species, there is significant variation in diapause lengths and behaviors (Higaki, 2016). This wide array of adaptive behaviors is a result of the coevolution between these insects and their various hosts. Here, we present a high quality genome of a Curculio species. With this genome, we discuss the genetic and evolutionary factors that have given rise to this unique life history of Curculio and future plans to compare the genomes of multiple Curculio to further unravel this mystery.

Authors: Erika Frandsen. Mentors: C. Riley Nelson. Insitution: Brigham Young University. The Timpanogos Cave System consists of three caves in American Fork, Utah. In 2003, the National Park Service (NPS) commissioned Dr. Riley Nelson and laboratory students to conduct a survey of the diversity and abundance of terrestrial arthropod species found within the caves. Over the two years they sampled in Timpanogos Caves, they identified 55 species of macroinvertebrates in an unpublished report.In the 20 years since the last sampling, many environmental factors could’ve changed in these caves, contributing to an overall decrease in both abundance and biodiversity of fauna. Therefore, we repeated the sampling done by the Nelson lab in 2003-2004 for the duration of May 2023-October 2023 to compare the two time periods’ macroinvertebrate communities.The six taxonomic families found most abundantly in 2003-2004 continue to be the most abundant families found in 2023. Additionally, a new taxon of cave beetle Rhadine sp. not found 20 years ago was discovered early in our sampling of 2023. Despite the increase in visitation over the past 20 years, at this stage in the analysis, the macroinvertebrate community within the caves seems to have almost no loss of diversity. Additionally, the appearance of new taxa indicates that there is still more life and diversity within the caves left to discover.

Authors: Nathaniel Call. Mentors: Chelsea Romney. Insitution: Brigham Young University. New college students may experience stressors like difficult academic work, new social environments, and living on their own for the first time. Students of color may experience additional stressors due to minority stress, experiences of racism, and unfamiliar social contexts. We collected self-reported depression, anxiety, and academic self-efficacy measures from 742 first-year college students. We found that students of color reported higher levels of depression, F(1, 733) = 11.04, p < .001, and anxiety F(1, 734) = 14.91, p < .001, compared to white students. Further, we found that white students reported higher academic self-efficacy, F(1, 742) = 9.97, p = .002, compared to students of color. This suggests that lower confidence in academic work is related to higher depression and anxiety in nonwhite students, r(661) = -.32, p < .001. Our study builds on previous research by providing a possible pathway through which students of color develop more depression and anxiety through lower academic self-efficacy compared to white students.

Authors: Nicholas Poll. Mentors: Moriel Zelikowsky. Insitution: University of Utah. Social interaction is one of the chief drivers of evolution and natural selection. Animal interactions shape the ecological world and have shaped the world’s evolutionary timeline. Of the many types of animal interactions, one of the most influential to behavior and development is aggression. Many animals' aggressive behaviors and mechanisms are so distinct that their origins can be traced back to specific selective pressures. These pressures range from reproductive competition to predator-prey interactions and have apparent effects on many species’ developments. There are many stressors that can increase aggressive tendencies found in mammals, yet it’s clear that the neural bases that connect these stressors and their behavioral responses are understudied. The targeted brain region in this project is the bed nucleus of the stria terminalis (BNST). The BNST is a neurochemically diverse node of the extended amygdala that has been implicated in mediating stress and sensory information. It anatomically acts as a relay station between a number of different structures including the hypothalamus and amygdala. The BNST has been implicated to have a role in stressor-induced aggressive behaviors; however, the neural mechanisms that connect this region and these behaviors are relatively understudied. Corticotropin-releasing Hormone (CRH) in mammals displays upregulation following the introduction of a stressor and has been found to mediate aggression and anxiety. Similarly, tachykinin-expressing neurons have been shown to have a role in mediating aggression in mammals. While CRH and tachykinin’s link to anxiety and aggression is well supported, it is not clear to what extent it has on BNST. This project aims to provide data to support the role that CRH and Tachykinin 2 (Tac2) in the BNST have in mediating aggression in mice through optogenetic stimulation.

Authors: Jared Carter. Mentors: Jeff Tessem. Insitution: Brigham Young University. Type 2 diabetes mellitus (T2D) is a chronic condition affecting nearly half a billion people worldwide. Symptoms of T2D include impaired glucose tolerance, decreased insulin secretion and significant weight gain. While the symptoms of T2D are well-documented, the underlying pathology remains unclear. Recent research has indicated the critical role of the nuclear receptor Nr4a3 in the development of glucose intolerance and weight gain. In individuals with T2D, the Nr4a3 promoter is hypermethylated, leading to decreased Nr4a3 expression. Elucidating the role of Nr4a3 in mitochondrial respiration in adipose will help define the mechanism of T2D onset and treatment. I studied mice with full body knockout (KO) for Nr4a3. These mice exhibited T2D-like symptoms, including impaired glucose tolerance, reduced insulin secretion and increased adiposity. I measured mitochondrial respiration in muscle, liver, kidney, and adipose tissue, with impaired respiration only observed in adipose tissue. This impairment in adipose tissue respiration correlated with an increase in the size of all adipose deposits, larger adipocytes, and expanded lipid droplets. Intriguingly, the analysis of electron transport chain and tricarboxylic acid complex proteins revealed no significant differences compared to control samples. Instead, the change in respiration was attributed to a reduction in active DRP1 protein, responsible for mitochondrial fission and maintenance. These findings have important implications for our understanding of T2D and its potential treatment strategies, which will be discussed in more detail.

Authors: Dallin Oyler, Ana Alfaro, Kailee Myxter, Ben Brooks, Amanda Brooks. Mentors: Jessica Pullan. Insitution: Southern Utah University. Nausea and vomiting are common complications that occur in 70% of pregnancies. Hyperemesis Gravidarum (HG) is the most severe form of these symptoms and is estimated to be prevalent in 0.3-2% of pregnancies. Due to the fetal and maternal morbidity associated with HG, identifying the cause and treatment options for these women is a critical task in obstetrics. Research regarding the etiology of HG has been fairly recent and is still ongoing, however, evidence had directed to a positive correlation between increased levels of the serum protein GDF15 and HG symptoms. We hypothesize that polymorphisms in both maternal and fetal DNA plays a role in the upregulated GDF-15 seen in mothers experiencing symptoms of HG during pregnancy. The DNA of 2 mothers and their corresponding children were sequenced and analyzed. The DNA was obtained through buccal swabs from the epithelial cells of the inner cheek, and then purified and ran through PCR. We employed 3 distinct primers that correspond to mutations in the genome that account for the elevated levels of circulating GDF-15 in the mother. As of current, the mother-daughter DNA is still under analysis for single-nucleotide polymorphisms, however recently published literature has suggested results similar to our hypothesis. M. Fejzo et al. shows that upregulated serum GDF-15 is primarily of fetal origin, and that maternal sensitivity to GDF15 increases the risk for developing HG. Additionally, the DNA coding variant GDF15 C211G was shown to elevate the risk of HG

Authors: Auriana Dunn. Mentors: Kathryn Sokolowski. Insitution: University of Utah. Reconstructing the diet of extinct animals can help us understand how living species are suited to their environments, which can inform paleoecological and zooarchaeological research as well as modern species conservation. Seasonal variation in animals’ diets can further be used to reconstruct seasonality patterns in the past and understand how animals may vary their diet under future climate change. However, determining the diet of extinct species is challenging, and detecting seasonal changes in diet is even more so. Dental microwear texture analysis (DMTA) can be a tool to solve this problem. DMTA analyzes dental microwear by measuring the pits and scratches on teeth to determine what an animal consumed in the days and weeks before it died. This project examines the DMTA of modern African Bovidae species to determine if seasonal dietary changes can be observed. If a difference can be found in the dental microwear of specimens that died during different seasons, seasonal changes in diet can be inferred. Here, I gathered dental microwear data from 407 specimens across five modern studies and matched the data to the museum specimens’ date of death to determine whether it died in a wet or dry season. The analysis found that four of the Bovidae species examined in our study showed evidence of seasonal diet shifts in their DMTA data. Dental microwear can be useful in determining seasonal diets and season of death for specimens. This method can be applied to extinct Bovidae species to understand ancient ecosystems and the evolution of Bovidae dietary strategies.