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Cultural humility impacts on premature termination in psychotherapy

March 21, 2024 12:00 AM
Authors: Logan Walker, Kate Coneys, Nick Tops, Russ Bailey. Mentors: Russ Bailey. Insitution: Utah Valley University. Background: Cultural humility is an influential concept in Multicultural Orientation therapeutic research (Owen et al, 2011). Cultural humility is defined as “a lifelong process of self-reflection and self-critique whereby the individual comes to value and respect cultural diversity, recognizes his or her own biases, and is open to learning from others" (Tervalon & Murray-Garcia, 1998, p. 117). Cultural humility practices attempt to address cultural differences in therapy. Therapy absent of cultural humility can run the risk of failing the needs of culturally diverse clients. Racial and ethnic minorities are more likely to experience premature termination (Owen et al., 2015). Premature termination is defined as when a client begins an intervention but discontinues prior to recovering from the problem that he or she sought treatment for against provider recommendations (Swift & Greenberg, 2012). Premature termination is a significant concern as those who do not complete an intervention are less likely to experience symptom relief or meet their treatment goals (Steinberg et al., 2019). hope to better understand the link between cultural humility and premature termination/treatment deterioration in therapy in college students. We hypothesize that lower therapist’s cultural humility will be associated with an increased likelihood to unilaterally terminate therapy prematurely or show treatment deterioration. Methods: Data was collected from students who participated in therapy at the Utah Valley University Student Health Services from 2021-2023. The sample size for this dataset is approximately 560 participants. We will use two different operationalizations of premature termination/treatment deterioration: 1. Clients who reported have attended therapy for at least 3 sessions, has at least 1 cultural humility datapoint, and reported feeling “worse than at intake” on an item of subjective progress during at the last attended session with no future sessions scheduled for at least 90 days. 2. Clients who scored worse or the same on well-being indictors (PHQ-9 and GAD-7) and reported feeling “the same as intake” or “worse as intake” on an item of subjective progress during the last attended session with no future sessions scheduled for at least 90 days.. Clients completed the Cultural Humility Scale (Hook, 2013) and microaggression items at the beginning of their 3rd session and then every 4 sessions (e.g., session 7, 11, etc.). Clients completed the PHQ-9, GAD-7, and subjective progress items at the beginning of every session. We will use logistic regression to see how cultural humility scores and microaggression frequency/impact predict premature termination/treatment deterioration. Results: Results from the analyses will be presented during the poster presentation Discussion: The results of the study will help us determine if therapist's cultural humility is related to client premature termination. If we find support for our hypothesis, this means that cultural humility is an important factor in therapeutic processes that has direct impact on the effectiveness for therapy interventions. However, rejecting our hypothesis may suggest that cultural humility is not an important aspect of premature termination. Limitations of this study include potential ceiling effects due to therapists striving to be cultural humble and thus limiting the variability in our sample.

The Influence of Political Affiliation on Academic Performance: An Examination on How Familial Political Beliefs May Impact a Child’s Educational Outcomes

March 21, 2024 12:00 AM
Authors: Ellery White. Mentors: Yi Yin. Insitution: Utah Valley University. This study will examine the influence that parental political beliefs may have on students’ academic performance. Decades of research have formed empirical links between social- contextual factors and student academic achievement, and this paper will seek to identify whether parental political values play a role in the academic achievement of their children (Lee and Shute 2010; Yamamoto and Holloway 2010). Parental attitudes, behavior, and stylistic approaches to their child’s rearing and education have been extensively studied for how they impact educational outcomes (Pandey and Thapa 2017). This study fulfills the gap in research on the association between parental political beliefs and children’s academic performance. This adds to the academic discourse on the impact parents can have on their children in an academic setting by answering the following questions: How can the political beliefs of parents impact a students’ way of thinking and therefore ability to perform in school? Does the extremity of the parents’ political beliefs make a difference in the outcome of the child’s academic performance? A survey was distributed to a random sample of UVU students which measured their academic performance and the political beliefs of their parents. The statistical program SPSS will be used to conduct a multiple regression analysis to examine the relationship between the independent variable (parents’ political beliefs) and dependent variable (students’ academic performance) to see if there is an association between the two. I hypothesize that students with parents who have strong political beliefs are more likely to be impacted in their academic performance than students with parents who do not have strong political beliefs. Discovering the impact of parental political belief will open several important avenues of inquiry related to educational outcomes of students.

Mayfly Wing Wonders: Uncovering Genetic Threads in Parthenogenic Individuals using RNA

March 21, 2024 12:00 AM
Authors: Melissa Rangel, Avery Larsen, Heath Ogden. Mentors: Heath Ogden. Insitution: Utah Valley University. Mayflies, also known as ephemeroptera, are members of the first group of insects that evolved the ability to fly(Gillott, 2005). Distinct developmental characteristics of the life stages of the mayfly are the reason that Ephemeroptera are of particular interest.The aquatic nymph stages do not have wings, but they do possess abdominal leaf-shaped gills, and in some cases gills associated to the legs. The abdominal gills can sometimes be used to help propel the insect through its environment (Eastham, 1936) in addition to performing gas exchange. Centroptilum triangulifer (family Baetidae), will be used for RNA extractions to study the developmental genetic profiles for characteristics such as wings and gills. Objectives for this research are 1). Acquire samples from different instars, subimago, and imago. 2) Perform 10 RNA extractions. 3) Use Qubit technology to ensure ≥ 20 ng/μL RNA concentration per ≥ 10 μL. 4) samples will be packaged in thermo-stable shipping boxes and sent to Novogene where samples are tested once more for quality control. 5) Once quality is confirmed, samples will be sent and processed by Novogene. 6). Using an in-house bioinformatics workflow, RNA data will be checked for quality, trimmed, aligned, and assembled. The assembled contains will be used to search for genes of interest. 7) the resulting RNA expression profiles will be compared across the installs, sub imago, and imago and to other expression profiles from other related insects.

Brain-eating amoeba rescue-of-infection model using drug combinations

March 21, 2024 12:00 AM
Authors: Karissa Bauer, Kennedy Lewis, James Belnap, Andrew Torena, Braden Freestone, Luis Montenegro Calla. Mentors: Daniel Clark. Insitution: Weber State University. In the United States, 157 documented cases of primary amoebic meningoencephalitis (PAM) have been recorded between 1962 and 2022. In the 60 years since the first reported case, only four patients have survived; a 97.5% fatality rate. The causative agent responsible for the highly fatal infections, is the free-living, single-celled parasite, Naegleria fowleri— or the “brain-eating amoeba.” The aquatic thermophiles are opportunistic pathogens that inhabit a wide range of environments; from poorly maintained pools, moist sediment, rivers, lakes, etc. Exposure to N. fowleri invasion is reliant on direct contact with a host’s upper nasal mucosa, and the active parasite. Rapid deterioration of neural tissues occurs shortly after infection.Today, there is no universally accepted treatment plan for treating PAM infections. The CDC has recommended an empirical approach to treatment, and suggests utilizing combination drug-therapies. Suggested drugs include Amphotericin B, Azithromycin, Fluconazole, Miltefosine, and Rifampin. To better understand and evaluate the effects of these drugs on N. fowleri, a rescue-of-infection model was applied to test the efficacy of single and combination-drug treatments, at various time points. The model utilized an immortalized HeLa cell line that was infected with the amoebae until roughly 50% cytotoxicity was observed. The rescue stage commenced with drug additions, and HeLa cell metabolism, and apoptosis levels were monitored using fluorescence viability assays and caspase-3 enzyme detection systems. Drug combinations that included Rifampin and Amphotericin B, provided the greatest protection for human Hela cells against N. fowleri infections. Data collected from this model provides deeper understanding of Naegleria fowleri pathogenicity. This knowledge will aid the development of more reliable and efficient treatments for this devastating infection.

Relationship with soul: a short excursion in psychology and religion

March 21, 2024 12:00 AM
Authors: Genna Howard, Cameron John. Mentors: Cameron John. Insitution: Utah Valley University. In the history of psychology, the concept of soul played a significant role informing philosophy and theory. In the late 1800’s many questioned the concept of soul in favor of measurable concepts that aligned with the scientific method, distinguished from religious classification that were deemed unworthy of pursuit. In the emergence of the science method, the concept of soul faded and the field of psychology experienced soul-loss, becoming soul-less. After nearly 150 years, there is a movement in the field advocating for soul to return to its former status by shedding light back onto the soul. For some, soul is aligned with religious thought. Religions are experiencing a loss of congregants, members engaging in faith transitions, and experiencing soul-loss. This paper will compare and contrast what happened in psychology with the experiences of some in religious organizations while looking at how the emotion of fear plays a part in both organizations. The methodology utilizes historical concepts and experiences shared by the brave souls who are challenging the current beliefs in both fields. In addition, anecdotal, informal information provided by individuals close to the authors is used to emphasize the practical application of this crisis in psychology and religions with the goal of reigniting a passion for soul.

Change Over Time: Cultural Humility and Microaggressions in Therapy

March 21, 2024 12:00 AM
Authors: Kate Coneys, Nick Top, Russ Bailey, Logan Walker, Julia Toomey. Mentors: Russ Bailey. Insitution: Utah Valley University. Background: Past literature has emphasized the importance of practicing culturally competent care and has shown that higher clinician cultural humility, as well as a lower frequency of microaggressions occurring in therapy, have been associated with more desirable therapeutic outcomes in retrospective, cross-sectional studies. However, research has also shown that patient outcomes appear to be unrelated to their therapist's experience level (Erekson et al., 2017), suggesting that more experience may not lead to being a more effective therapist, including more culturally humble. Despite the breadth of research on the significance of cultural competence, less is known about how a therapist's cultural humility changes across treatment with a particular client, or how a therapist’s cultural humility changes over an extended period. The aim of the current study is to examine this concept by analyzing both the relationship between the number of sessions spent working with a given therapist and a client’s rating of the clinician’s cultural humility, as well as the relationship between the average cultural humility rating of the therapist and time. We will also examine how the impact of microaggressions occurring in therapy changes during the course of therapy. Hypotheses: It is hypothesized that the results of this study will show a positive association between the number of sessions with the same therapist and their cultural humility, as rated by the client, as well as a negative association between the number of sessions and the impact of microaggressions reported by the client. Additionally, it is hypothesized that the therapist’s cultural humility rating over the entire course of data collection will not change to a statistically significant degree. Methods: We used archival data collected by Utah Valley University’s Student Health Services Counseling Center from August 2021 – September 2023. This archival dataset consists of 564 clients with at least one cultural humility and microaggression data point. Beginning at the third session, and occurring again every four sessions, clients were asked to rate their clinician’s cultural humility, using The Cultural Humility Scale (Hook et al., 2013), and report the frequency and impact of microaggressions that had occurred during the last four sessions. We will analyze the data using multi-level latent growth modelling. Results: Results will be presented as part of the poster presentation. Discussion: If the results of this study agree with the hypothesis, the implications may be that cultural humility improves over time with specific clients, potentially explained by growth of therapeutic alliance over time. However, it may also imply that a therapist’s cultural humility does not improve over extended time periods, showing that cultural humility appears to be static rather than dynamic. If the results do not agree with the hypothesis, this may imply that a client’s perception of their therapist’s cultural humility is static, but that over extended periods of time a therapist’s cultural humility can improve, implying that it is trainable. Sources: Erekson, D.M., Janis, R., Bailey, R.J., Cattani, K., Pedersen, T.R. (2017). A longitudinal investigation of the impact of psychotherapist training: Does training improve client outcomes? Journal of Counseling Psychology, 64(5), 514-524. https://doi.org/10.1037/cou0000252 Hook, J.N., Davis, D.E., Owen, J., Worthington, E.L., Utsey, S.O. (2013). Cultural humility: measuring openness to culturally diverse clients. Journal of Counseling Psychology, 60(3), 353-366. https://doi.org/10.1037/a0032595

UTAH'S HERITAGE: A LITTLE KNOWN STORY OF MORMONISM AND EUGENICS

March 21, 2024 12:00 AM
Authors: Lindsay Beekman. Mentors: Olga Kopp. Insitution: Utah Valley University. This research probes the primary historical newspapers of early 1900s Utah, providing insights into the cultural atmosphere of the time and the widespread support for the Eugenics movement throughout the state. Our findings indicate that out of 72 distinct newspaper articles discussing Eugenics in Utah between 1908 and 1938, 62 conveyed a positive perspective, 9 remained neutral, and only 1 expressed opposition. Notably, from 1947 to 1996, leaders of The Church of Jesus Christ of Latter-Day Saints administered the ‘Indian Student Placement Program’ or ‘Lamanite Placement Program,’ aligning with many Eugenics principles. Utah, like many places worldwide, embraced Eugenics with open arms.

Deciphering the Impact of Pomc Mutation on Mouse Reproductive Behavior: A Comprehensive Data Analysis Approach

March 21, 2024 12:00 AM
Authors: Lauren Silvatti.. Mentors: Zoe Thompson. Insitution: Utah Valley University. Proopiomelanocortin (Pomc) deficiency, stemming from a mutation in the Pomc gene, presents a myriad of health challenges, including extreme hyperphagia, early-onset obesity, and infertility. This study leverages a mouse model exhibiting Pomc-deficiency to delve into the root causes of infertility. While the correlation between obesity and fertility is well-established, our primary focus is to discern whether the POMC mutation independently contributes to reproductive challenges. Our investigation extends into the interplay of genetics, endocrinology, and obesity through data analysis using R, with an emphasis on deploying advanced statistical models.Video recordings of sexual behavior interactions have been analyzed manually, with independent observers marking each behavior. The frequency and duration of each behavior will be compared among three groups of pairings: wildtype-wildtype, heterozygote-heterozygote, and homozygous POMC-deficient mice. Techniques will include data cleaning, timestamp manipulation, and behavior categorization in R, a programming language commonly used for data analysis. To discern patterns and variations, our analysis will also employ statistical models such as linear mixed-effects models. We can account for potential confounding variables and significant differences in durations of previously identified important reproductive behaviors. Visualization tools, including box plots and violin plots, will provide an initial glimpse into the distribution of behavior durations. Subsequently, we plan to conduct inferential statistics, employing techniques such as Analysis of Variance (ANOVA) to assess the significance of differences across multiple groups. Our investigation extends beyond descriptive statistics, with a focus on predictive modeling. Regression analyses will explore potential relationships between behavior durations and reproductive success. Machine learning algorithms will be applied to uncover complex interactions within the dataset.The anticipated results promise not only a nuanced understanding of the interplay between Pomc mutations and reproductive challenges but also the identification of potential biomarkers or predictors of successful reproduction. This comprehensive statistical approach contributes significantly to the fields of genetics, endocrinology, and obesity research, offering a robust framework for future investigations into the intricate relationship between genotype and behavior.

We Are Not Alone: Examining the Impact of a Tween-Teen Diabetes Day Camp

March 21, 2024 12:00 AM
Authors: Heidi Blaylock, Carter Leuba, Eddie Hill, Christina Aguilar, Carla Cox, Aiden Hill. Mentors: Eddie Hill. Insitution: Weber State University. Type 1 diabetes (T1D) is a chronic disease that influences all health aspects. The self-determination theory (SDT) suggests that three psychological needs of competence, autonomy, and relatedness are necessary for motivation to engage in healthy behaviors. Through medical specialty camps, these needs can be met by educating campers on how to manage T1D and realize they are not alone. The volunteer-based, five-day, inaugural REACH teen/tween camp for youth with T1D was held at WSU. Camp activities were engineered around the three basic needs described by the SDT. These needs were promoted by physical and educational activities, and meeting friends. A pre and post evaluation was given. Two measures were significant with autonomy being the greatest, (M=3.93, SD= .75) to post test (M=4.49, SD= .56), with t(25) = -6.258, p= <.001), effect size r = 1.2 Blood glucose levels were collected through an online platform that allowed staff to monitor campers’ levels. The week's average blood glucose level was 152 mg/dL. This study explored the use of SDT to examine the effectiveness of a diabetes camp for youth and hopefully result in better physical and emotional health thus mitigating the risk of complications.

Mountain Haven - Senior Capstone

March 21, 2024 12:00 AM
Authors: Brandon Rojas. Mentors: Kristen Arnold. Insitution: Weber State University. The Mountain Haven building in Salt Lake City, a mixed-use facility, offers a wide range of services, including housing, childcare, and healthcare. The Single Parent Project, a non-profit organization, is working to provide suitable housing solutions for single parents and their children. The primary challenge is creating a secure, private, and healthy living environment that supports the emotional, financial, and mental growth of single parents and their children. To address this challenge, the Mountain Haven building must be designed to meet the unique needs of single-parent families. This includes providing access to quality childcare, flexible work arrangements, and efficient household management solutions. It is also important to foster a sense of community and neighborliness and to ensure that single parents have access to affordable and safe housing in a safe neighborhood (Johnson, 1986). Additionally, the arrangement of the home should be flexible to accommodate both work and childcare responsibilities. The design of the Mountain Haven building should also consider the latest research on daycare centers, healthcare facilities, and interior design. This includes using materials that are free of harmful substances, creating spaces that promote learning and nurture children's development (Monoko, Mukurunge, Bhila, 2021), and using light and color to create a healing environment for parents and children to reduce stress and strengthen the mood, mind, and body. (Khaleghimoghaddam, 2023) By incorporating these principles into its design, the Mountain Haven building can provide single parents and their children with a safe, supportive, and nurturing environment in which to thrive.

Investigating the Utility of Avatars and Actors in Undergraduate Psychology Role-Play Exercises

March 21, 2024 12:00 AM
Authors: Kennedy Parker. Mentors: Melinda Russell Stamp. Insitution: Weber State University. Role-playing is considered one of the most effective teaching strategies for training undergraduate clinical psychology students because it fosters active learning in a supportive and safe environment (Gibbs, 2019; Osborn & Costas, 2013). The literature is replete with studies that document the benefits of role-playing for clinical training. These benefits include the development of active-listening, perspective-taking, empathy, critical thinking, communication, collaboration, problem-solving, and increased enjoyment of course material (Dawood, 2013; Gibbs, 2019; Grose-Fifer, 2017). A challenge associated with classroom role-plays involves student reluctance to participate due to feelings of self-consciousness and anxiety associated with being negatively evaluated (Mallonee, 2018). There is limited research on the effectiveness of different role-play modalities which may include classmates role-playing with one another; as well as actors and virtual avatars role-playing with students. There have been mixed findings in the literature regarding whether students are more comfortable disclosing to virtual avatars or to actors in face-to-face encounters (Rogers et al., 2022; Roster & Chen, 2016). To date, there are no research studies comparing student perceptions of role-plays with virtual avatars to face-to-face interactions with actors in undergraduate psychology classes. For the current study, 60 undergraduate Psychology students were randomly assigned to either role-play with an avatar or an actor. Following the 25-minute role-plays, students completed surveys assessing perceived utility value, self-efficacy, level of anxiety, engagement, and future interest in role-playing. This preliminary study will compare the effectiveness of avatar and actor role-play modalities which will help educators better structure training for undergraduate students in clinical psychology.

Transdermal antiseptic products as a method to decrease skin bioburden prior to surgery

March 21, 2024 12:00 AM
Authors: Kiersten Gardner, Hannah Duffy, Abbey Blair, Nicholas Ashton, Porter Stulce, Dustin Williams. Mentors: Dustin Williams. Insitution: University of Utah. Transdermal antiseptic products as a method to decrease skin bioburden prior to surgeryKiersten Gardner(1,2), Hannah Duffy(1,2), Abbey Blair(2), Nicholas Ashton(2), Porter Stulce1(2), Dustin Williams(1,2,3,4)1 Department of Biomedical Engineering, University of Utah, Salt Lake City, UT2 Department of Orthopaedics, University of Utah, Salt Lake City, UT3 Department of Pathology, University of Utah, Salt Lake City, UT4 Department of Physical Medicine and Rehabilitation, Uniformed Services University, Bethesda MDIntroduction: Preoperative skin preparation (PSP) kits are used before surgery to prevent surgical site infection (SSI). These kits consist of alternating scrubs of alcohol and Chlorohexidine Gluconate (CHG) or Povidone Iodine (PVP-I). Transdermal antiseptic products like Ioban, Tegaderm, and Surgiclear are also used clinically to eradicate skin bacteria by releasing antiseptic over time. Despite these precautions, infections often occur, usually stemming from a patient’s endogenous skin flora. The bacteria causing SSI reside deep in dermal sweat glands and hair follicles, untouched by traditional PSP. To eliminate these bacteria, antiseptics must diffuse deeper into the skin at concentrations above the minimum inhibitory concentration (MIC). We screened FDA-approved topical antiseptic products using a modified Kirby Bauer Assay to assess bacterial kill over time. We hypothesized that extended use of topical antiseptic products prior to surgery would kill deep-dwelling skin bacteria. We tested the extended use of these products on pig backs in conjunction with a CHG PSP to evaluate remaining bioburden. Methods:We determined the minimum inhibitory concentrations (MIC) for CHG and PVP-I against three common skin bacterial strains. We then took 6 mm biopsy punches of Tegaderm, Surgiclear, and Ioban and placed them on fresh bacterial lawns daily for 3 days. We measured the zones of inhibition (ZOI). Lastly, we applied the products to the backs of 4 Yorkshire pigs (n=4 products per test). After 48 h, we removed the products, performed a PSP- CHG scrub, and excised skin sections underneath the products. We homogenized the skin and quantified colony forming units (CFU)/g tissue. We analyzed the outcomes statistically using a mixed effects linear regression to determine significance.Results:The average MIC values for CHG and PVP-I were 2 and 2,000 ug/mL for Staphylococcus aureus, respectively. The average 24 h ZOIs for S. aureus were 7.9, 22.4, and 10.1 mm for Ioban, Tegaderm, and Surgiclear, respectively. The average log10 reduction for the CHG PSP, Ioban, Tegaderm, and Sugiclear were 1.87 ± 0.232, 1.65 ± 0.192, 1.69 ± 0.222, and 1.77 ± 0.316 CFU/cm2, respectively. The p values between the CHG PSP and Ioban, Tegarderm, and Surgiclear were 0.491, 0.572, and 0.746, respectively. Discussion:The benchtop data indicated adequate antiseptic diffusion and kill. In the porcine model, however; the presence of any of the products resulted in a statistically insignificant log reduction. Extended use of FDA-approved transdermal antiseptic products does not appear to decrease the skin’s bioburden. It appears that the products are not releasing high enough concentrations of the antiseptic to kill bacteria.

Re-Membering and the Role of Community In Exorcism In Toni Morrison’s Beloved

March 21, 2024 12:00 AM
Authors: Jen Hansen. Mentors: Nicole Dib. Insitution: Southern Utah University. Toni Morrison’s contribution to Gothic horror with Beloved expands the tradition into the history of human enslavement, specifically in the context of American history. She reconceptualized ‘remembering’ as both the conscious awareness of the past and the literal reassembling of members of the body, and by extension the family and the home. Morrison coined the term “rememory” in reference to the intentional act of recollection performed by an entire community. The characters of the novel are haunted in several ways and each haunting is only exorcized or overcome through acts of communion, or rememory — many of which are symbolically religious even if not sanctioned as such. The main character is a woman named Sethe, who is haunted by the ghost of the daughter she murdered rather than allow to be taken as a slave. The ghost of Beloved represents the return of the repressed trauma of her death and the connection to Sethe’s previous life in captivity. In order to exorcize Beloved’s ghost and free themselves from her oppressive presence, Sethe and her living daughter, Denver, must re-member the broken family structure within their home, and rememory the traumatic past with the support of the community in order to heal. American Gothic traditions in literature have long been used to reflect on anxiety, discrimination, and disempowerment related to the Other. In this novel, Morrison uses that tradition to give shape to the culturally specific legacy of slavery in America. In this presentation I examine the novel’s Gothic elements and the ways the community plays the part of the exorcist as an essential advocate for the physical and emotional survival of Sethe and Denver.

Artificial black holes: are they a threat to humanity?

March 21, 2024 12:00 AM
Authors: Tate Thomas. Mentors: Alexander M Panin. Insitution: Utah Valley University. We wanted to see if accidentally creating mini black holes in high energy particle collisions posed a real threat to humanity. To do this, we calculated some properties of such a black hole, such as its life span, radius, density, and minimum energy required. We found that it is unlikely to exist, let alone destroy the planet. Furthermore, we calculated what would happen if it were to exist, finding that it would move through the Earth with little resistance and with a small amount of Earth matter absorbed. Depending on initial velocity, the black either quickly escapes Earth or would settle orbiting it with the orbit part of which passes via Earth. It is interesting that in a simplified model of Earth as of a sphere of uniform density, the inner part of the orbit of black hole is also elliptical (as the outer is) but not Keplerian - with Earth center not at the focus but at the center of another ellipse. In the case of small initial velocity when entire orbit is inside Earth, the period of such inner orbit is constant regardless of birth location and initial velocity of black hole. The goal of this presentation is to discuss the results of our calculations and to explore potential applications to our understanding of interaction of mini black holes with ordinary atomic matter.

Quantifying Approximation Errors in the Flash Thermal Diffusivity Measurement Technique Using High Fidelity Simulations

March 21, 2024 12:00 AM
Authors: Tage T Burnett, Jakob G Bates, Christopher R Dillon, Matthew R Jones. Mentors: Christopher R Dillon. Insitution: Brigham Young University. In recent years, modeling and simulation have become more prominent in solving heat transfer problems. The accuracy and predictive power of heat transfer simulations is limited by the quality of the thermal properties used within the model. Thus, one method for improving computational accuracy is measuring thermal properties more precisely. Additionally, increased precision of thermal properties benefits other aspects of engineering including design and analysis. This research focuses on quantifying approximation errors in the widely adopted flash method for measuring thermal diffusivity. The flash method leverages several approximations to make it simple and easy to use; however, these approximations do not reflect reality and introduce measurement errors. Understanding these errors is critical for developing high-precision thermal diffusivity measurement techniques.In the flash method, the top surface of a small, cylindrical disc of material is subjected to a short pulse from a laser or flash lamp and the time-dependent temperature at the opposite surface is recorded. The thermal diffusivity is calculated using those temperature measurements in combination with a mathematical model. The accuracy of the flash method depends upon the accuracy of the mathematical model. One common mathematical model is the Parker Model. This model assumes that all of the energy from the pulse is deposited in an infinitesimally thin layer at the surface of the material and negligible heat is lost to the environment. These assumptions simplify the model, making it easy to use, but introduce errors into the measured thermal diffusivity.Computational methods can quantify these inaccuracies. Factors including heat lost to the environment, the temporal profile of the laser pulse, and the spatial distribution of the deposited energy can be incorporated into heat transfer simulations. Higher fidelity mathematical models can also be developed to account for these complexities. This project includes these and other factors to make simulations as realistic as possible. Various mathematical models, such as the Parker Model and higher fidelity models, are then used to calculate the thermal diffusivity from the resulting time-dependent temperature profiles and their measurements are compared to the simulated material’s true thermal diffusivity. Repeating this process for several material types will allow the precision of the models to be analyzed for each case. This analysis will be summarized at the conclusion of this project, providing a framework for developing more precise thermal diffusivity measurement techniques.

Subtle Force Communication for Intuitive Human-Robot Co-manipulation

March 21, 2024 12:00 AM
Authors: Samuel Charles. Mentors: Marc Killpack. Insitution: Brigham Young University. Robots have an incredible potential to help humans in extreme or dangerous situations due to their significant and consistent durability, strength, endurance, replaceability, etc. However, humans and robots move very differently, leading to difficulties working intuitively with a robot partner when completing a task such as lifting a heavy object. We recently conducted studies in which human subjects moved a 60-lb table to several different positions in a room; we recorded force and torque data, along with many other aspects of the movement. In these studies of human-human co-manipulation, we noticed a trend during particularly difficult maneuvers; when lifting the table to high positions or acute angles, subjects switched their hand holds on the table’s handles. This was likely an easier method of holding the table, but it may have also communicated placement, stability, understanding, and strength to the other partner, leading to a smoother and more intuitive movement and experience overall. If this is the case, this data could be used to help a co-manipulation robot both effectively understand the subtle commands in human movement and intuitively communicate needed movement to the human partner. This is particularly useful in emergencies like natural disaster sites and war zones, in which immediate help is needed, but there is no time to troubleshoot an unclear or unintuitive robot.

Exploring the Differences Among Attachment Styles with Cognitive Appraisal and Emotional Suppression

March 21, 2024 12:00 AM
Authors: Rebekah Hakala, Moroni Black. Mentors: Todd Spencer. Insitution: Utah Valley University. Over the years countless studies have delved into the theory of attachment due to its influence in the field of child development. Attachment theory is the idea that the sensitivity of parents or caregivers to a child’s bids for attention affect how the child will bond in relationships (Bretherton, 1992; Wilson-Ali et al., 2019). The style of attachment that a child has can affect them long-term and may influence other relational and developmental characteristics (Kurth, 2013). Due to the influence of attachment, our study investigates the relationship it has with cognitive reappraisal. Cognitive reappraisal is the ability to regulate one’s emotional states and the idea that a change in these thoughts is necessary to change negative emotions (Troy et al., 2017; Clark, 2022). With its ability to process and regulate emotion, cognitive reappraisal is another influential characteristic to the human psyche. Our results and analysis of the relationship between attachment theory and cognitive reappraisal come from a quantitative survey.The purpose of the present study is to examine the relationship among attachment styles and Cognitive Reappraisal. Our sample consists of 411 married individuals. Participants completed The Relationship Questionnaire (RQ; Bartholomew & Horowitz, 1991) and Emotion Regulation Questionnaire (ERQ; Gross & John, 2003). Results of the one way Anova were significant F (3,407) = 3.36, p=<.01. There was a significant difference in levels of cognitive reappraisal among attachment styles. Secure attachment (M=29.95, SD = 6.41), fearful attachment (M=27.27, SD = 5.92), preoccupied (M=27.99, SD = 7.27), and dismissing (M = 28.78, SD = 7.54). Results of the Bonferroni Post-hoc analysis indicated a significantly higher levels of cognitive reappraisal than attachment styles (p.<.001). Results provide empirical support that secure attachment styles tend to be beneficial for cognitive reappraisal.

Ransomware Resilience and Ethical Dilemmas: A Comprehensive Review of Threat Landscape, Impact, and Mitigation Strategies

March 21, 2024 12:00 AM
Authors: Asmaa Alsharif. Mentors: Sayeed Sajal. Insitution: Utah Valley University. Ransomware is malicious software which restricts users from accessing their computer and personal data through encryption. Ransomware attacks target a variety of victims including individuals, organizations, businesses, and governments. Attackers take control of this sensitive data and hold it hostage until a ransom is paid. Whether or not ransom is paid, the attackers’ damage can be irreparable. It includes but is not limited to exposing personal information, identity theft, blackmail, permanent deletion or corruption of valuable data, and Distributed Denial-of-service (DDos). Moreover, the financial implications of ransomware attacks are substantial. Victims face not only the direct costs of ransom payments, but also the expenses associated with system restoration and the potential fines for data protection violations. Furthermore, an ethical dilemma emerges from the response to extortion, as victims must grapple with the moral implications of potentially supporting criminal activities by paying ransoms to recover their data. This raises questions about the broader responsibility of stakeholders in cybersecurity and their duty to protect data privacy. The first ransomware attack emerged in 1989 and since then, ransomware attackers have persisted as a cybersecurity threat, striking at the core of data privacy. This paper delves into the multifaceted impact of ransomware incidents on data security, current ransomware techniques and strategies, and the ethical dilemmas they raise.Because ransomware attackers are constantly evolving their methods, it is important to routinely review the current literature to fully understand the tactics and techniques used by these cybercriminals. This research paper will review, analyze, and synthesize the existing literature about ransomware. It is crucial for individuals and organizations to be proactive, understand the nature of the threat, and take precautions to protect themselves and their data. This study encourages further research and discourse on the multifaceted impact of ransomware attacks on data privacy and the pursuit for effective countermeasures.

MRSA induced biofilm clearance by bacteriophage and antibiotic.

March 21, 2024 12:00 AM
Authors: Rainey Hughes, Avalon Marker, Elizabeth Bouwhuis, Yeshaswini Dudde, Bryan Dopp, Scot Carington, Jared Nelson. Mentors: Daniel Clark. Insitution: Weber State University. Antibiotic resistance is a pressing concern within the medical community as bacteria's resistance to antibiotics is escalating alongside the increased usage of antibiotics. According to the CDC, there are close to 2.8 million antibiotic resistant infections every year, with about 35,000 of them resulting in death. This issue has prompted antibiotic stewardship programs in clinics and hospitals to avoid adding to the list of resistant bacteria. Staphylococcus aureus, including the formidable methicillin-resistant S. aureus (MRSA) strain, poses a grave threat due to its antibiotic resistance. The challenges stemming from this resistance become even more formidable when these infecting bacteria assemble into biofilms. Biofilms are robust, adhesive layers composed of bacteria and their extracellular matrices of polysaccharides, proteins, and DNA. In clinical environments like hospitals, biofilms frequently develop on medical devices such as stents, catheters, and IV lines, as well as on metal and plastic surfaces of medical equipment. These biofilms exacerbate antibiotic treatments due to incomplete eradication; the most resilient bacteria persist after exposure. There is evidence indicating that bacteriophages, which are viruses that will a target particular species or strain of bacteria, have the ability to encode depolymerases. These depolymerases can identify biofilms, adhere to them, and subsequently break down extracellular polymeric substances. Furthermore, bacteriophages can produce lysins, which induce bacterial cell death through cellular lysis. These characteristics can potentially render the bacteria more susceptible to antibiotics. The use of bacteriophages can also be beneficial when it comes to the concern of opportunistic infections. Due to its selectivity to specific bacteria, it can attack the target hosts and leave the natural flora intact.In our research, we have induced biofilms in our bioreactor. With these biofilms we have been able to test different concentrations of multiple antibiotics, including Vancomycin, Oxacillin, and Carbenicillin in combination with phage K at different concentrations. Our research is aimed at showing a synergistic relationship between phage K and antibiotics, that will allow a subinhibitory concentration of both, in combination, to induce a complete kill and clearance. We have measured this by evaluating bacterial growth via absorbance measurements at 600nm in a Tecan plate reader. We have also measured biofilm clearance using the plate reader and measuring fluorescence at 630nm with a biofilm tablet assay. It was found that a subinhibitory concentration of antibiotic alone did not induce a complete kill and clearing, and that a subinhibitory concentration of phage alone did not induce a complete kill and clearing. However, once these concentrations were used in combination with each other, the complete clearing and killing of MRSA was achieved, and furthermore, was achieved with the antibiotic that the staphylococcus aureus is resistant to. Leading us to believe that we have found a renewed use for a currently ineffective defense mechanism.

Ethical Analysis of Web3 and Decentralized Internet

March 21, 2024 12:00 AM
Authors: Karli Kallas. Mentors: Jared Colton. Insitution: Utah State University. For this presentation, I will present a care ethics analysis of Web3 and decentralized internet, with a specific focus on privacy concerns and the increase of internet scams due to the lack of regulation and accountability. Ethics of care argues that there is moral importance in promoting the well being of care givers and receivers in a network of social relations, recognizing that all human life is co-dependent. It is important to note that care ethics was not designed to engage with such large networks of relationships, I will be expanding it to apply to the situation, so it gives us one new way of viewing the situation. I will expand on this ethical lens by including points from care ethics such as the empathy in the design of Web3, and responsibility for online communities. My research explores the intricate web of relationships and responsibilities in the decentralized environment, aiming to shed light on the vulnerabilities within internet scams in contrast to the benefits of cryptocurrency. I examine the moral dimensions of these scams, considering the impact on individuals and the broader digital community. The emphasis on care ethics demonstrates the interconnectedness of actors within Web3, as well as the need for a collective commitment to certain forms of innovation.

Efficiently and Accurately Simulating Coupled Nonlinear Schrödinger Equations with Exponential Time Differencing and Fourier Spectral Methods

March 21, 2024 12:00 AM
Authors: Nate Lovett. Mentors: Harish Bhatt. Insitution: Utah Valley University. Coupled nonlinear Schrödinger equations (CNLSEs) are an extension of the nonlinear Schrödinger equation (NLSE) that applies to multiple interacting wave systems. They occur naturally in many physical systems, including nonlinear optics, multi-component Bose-Einstein condensates, and shallow water waves. Solitons, which are self-contained, localized wave packets that preserve their shape and speed during propagation, are a significant application of CNLSEs. Solitons are prevalent in nonlinear systems and play a critical role in long-distance information transmission in telecommunications. Despite their widespread use in various fields, solving CNLSEs analytically is challenging, and numerical approximations are necessary. However, solving CNLSEs numerically is a difficult task because of their high nonlinearity.To overcome this challenge, in this presentation, we will introduce, analyze, and implement an established fourth-order Exponential Time Differencing scheme in combination with the Fourier spectral method for simulating one-dimensional CNLSEs. In order to check the performance of this method in terms of accuracy, efficiency, and stability, we will present simulation results on CNLSEs. Our results will consider single, two, and four soliton interactions for homogeneous Neumann, homogeneous Dirichlet, and periodic boundary conditions. The numerical results will show that the proposed method is able to preserve energy and mass for a long time simulation in soliton interactions, as well as preserve the expected order of convergence for the proposed method.

Rotational Dynamics of Thrown Food Items and Their Impact on Canine Catching Success

March 21, 2024 12:00 AM
Authors: Caleb Slade, Evelyn Davis, Michael Orr, Brevin Bell. Mentors: Vinodh Chellamuthu. Insitution: Utah Tech University. Our study examines a viral video featuring a dog named Fritz who consistently fails to catch thrown food items. This research aims to determine whether Fritz's inability to catch is a result of his owner's throwing technique or Fritz's own inherent clumsiness. To investigate this, we employ a mathematical model based on kinematic differential equations to analyze the rotational dynamics of various geometrical shapes that simulate the thrown food items. By pinpointing moments in the projectile trajectory where the food items are most easily caught, we provide empirically-based recommendations aimed at improving Fritz's success rate.

Using Ecological Niche Modeling to better understand Pediomelum aromaticum, a threatened Utah plant

March 21, 2024 12:00 AM
Authors: Ian Eggleston, Ashley N Egan. Mentors: Ashley N Egan. Insitution: Utah Valley University. Ecological Niche Modeling (ENM) is a very useful technique that gives us insight into a species’ present and possible future ranges, habitats, and niches. ENM has applications within conservational biology as models can be used to understand the extent to which climate change may impact a species. Additionally, ENM can be useful for prospecting for and propagation of rare plant species. This project will use ENM to create predictive range models for a rare plant species, Pediomelum aromaticum, with the goal of defining an ecological niche, determining impact of climate change, and general conservation of P. aromaticum. Here, we will compare 19 bioclimatic variables using correlation analysis and ecological niche modeling to determine which are the most impactful on the range of P. aromaticum. Additionally, ENMs will be created using the MAXENT algorithm from historical and predicted future climate data. By comparing these models, we can hypothesize as to how climate change may impact P. aromaticum. Finally, models will be compared between predicted climate futures defined as shared socio-economic pathways or SSP. SSP models estimate the impacts of human interactions within the scope of environment, governments, and each other to estimate the impact of varying sets of human interactions as defined by modified global behaviors within humanity and the impacts on global climate change. Different ENM models will be created with different SSP climate models so that we can understand how actions taken by humanity right now may impact the critically imperiled species P. aromaticum.

Effect of Print Parameters on tensile and flexure strength of 3D printed plastic

March 21, 2024 12:00 AM
Authors: Baylee Schumacher, Ryley Horrocks, Divya Singh. Mentors: Divya Singh. Insitution: Utah Tech University. 3D printed plastics have gained immense popularity in the recent times given the direct integration with CAD based software platform as well as ease of manufacturing. In addition, use of 3D printed parts can be more economical and less labor intensive.However, the mechanical behavior of these plastics is not well defined. There are many factors that affect the integrity of 3D printed plastic – extrusion temperature, infill pattern and infill percentage being a few of them.In this work, authors have attempted to study the effect of these factors on the tensile and flexure (bending) strength of 3D printed plastics. Firstly, tensile and bending samples are prepared by varying on more of the following – extrusion temperature, infill patter and infill percentage. Further, the specimens are tested in a destructive manner for tensile and flexure strength following the standard tensile and three-point bending technique on a Materials Testing System. The results on the variation in the strength as a function of print parameters provides an insight on the importance of standardizing these parameters to maximize strength and minimize cost.Keywords: additive manufacturing, 3D printed plastic, tensile strength, flexure strength

Effect of a supernova explosion on the mechaincal and thermal stability of orbiting planets

March 21, 2024 12:00 AM
Authors: Alexander Panin, Benjamin Miera. Mentors: Alexander Panin. Insitution: Utah Valley University. Recent searches for extrasolar planets have brought a surprising discovery – almost any star seems to have a planetary system around it. We know that massive stars end their lives in a violent supernova explosion, during which an extremely large amount of energy (~3x10^46 J) is released from the star in a very short time. In this presentation, we analyze the effect of this explosion – primarily the impact of the neutrino flash, the gamma ray flash, and the expanding plasma shell - on the mechanical and thermal stability of an orbiting planet. Our calculations show that a planet's orbit can be significantly disrupted by the momentum of the exploding star shell (depending on the planet's mass and proximity to the host star), but the radiation pressure from the explosion has a much weaker effect. If a star loses too much mass to the expanding shell, any previously stable orbit will become unstable, causing the planets to escape. Additionally, we found that the gamma ray flash and the plasma shell incident on the planet can cause significant heating, while the neutrino flash would have virtually no effect. Also, if a star’s collapse is asymmetric then the star itself can leave the planetary system due to the momentum of the asymmetric neutrino radiation. The sequence of events during a supernova explosion and how they influence such a planet is discussed in the presentation.

Detecting Antibiotic Resistance Genes in The Environment

March 21, 2024 12:00 AM
Authors: Natalie Welsh, Lauren Brooks, Skyler Belka. Mentors: Natalie Welsh, Lauren Books. Insitution: Utah Valley University. When bacteria gain resistance to antimicrobial substances, they become a public health threat due to an inability to treat infections and control outbreaks. Staphylococcus aureus is a common element of the human microbiome that is widely associated with antibiotic resistance. While MRSA (or methicillin-resistant S. aureus) has long been prevalent, new types of antibiotic resistance, including VRSA (or vancomycin-resistant S. aureus), have become an increasing threat in recent years. This research aims to collect samples and use polymerase chain reaction (PCR) to screen for genes associated with resistance to common antibiotics, such as erythromycin, tetracycline, methicillin, and vancomycin. Molecular biology techniques are used to test various resistant genes. First, sample collection uses FloqSwabs dipped in phosphate-buffered saline and swabbed on various locations on the UVU campus (e.g., door handles/knobs, vending machines, elevators, stair rails, water fountains, cash registers and keypads, countertops). Then, DNA from collected swabs was extracted using the DNeasy PowerSoil Kit. PCR was used to amplify the 16S rRNA gene to act as a positive control and confirm the methodology captured bacteria. Once confirmed, we also examined the samples for various antibiotic-resistant genes (e.g., ErmA, ErmC, TetM, TetT, etc.). Lastly, amplified DNA is visualized using electrophoresis gels, observing under UV light to verify amplification. Understanding the genes found on campus and where they are most prevalent will help elucidate the risks to students on UVU’s campus, providing valuable public health knowledge.

Detection of Beet curly top virus in Utah plants

March 21, 2024 12:00 AM
Authors: Elise Bennett, Megan Frisby, Rob Hess, Max Taylor, Erin Riggs, Alma Laney. Mentors: Alma Laney. Insitution: Utah Valley University. Beet curly top virus (BCTV) is a highly destructive virus found in most of the Western United States characterized by curling of the leaves, yellowing, and lack of produce. Historically, BCTV was a key factor in the decline of sugar beet production in Utah, formerly a large factor in the state economy. Commonly found in beets, tomatoes, and peppers, BCT is vectored by the beet leafhopper, Circulifer tenellus, as it feeds on the phloem tissue. Novel hosts of this virus found in Utah include sunflower, mallow, eggplant, hemp, big sagebrush, and Solanum jamesii, the Four Corners potato. Sunflowers showed symptoms of curled leaves and stunting. Similarly, the eggplant developed shriveled leaves and a mosaic at the leaf tips. Four Corners potato, a staple food source of the Indigenous peoples of the Southwest, displayed strong symptoms including stunting, leaf yellowing, and leaf curling. Weedy species and other garden plants displaying various viral symptoms were also collected for testing. Detection was done using published BCTV detection primers and rolling circle amplification with the use of Phi29 polymerase to prepare the genome for whole genome sequencing. A 518 bp region was sequenced to 4x coverage from eight host samples. To ensure the validity of diagnosis, a triple antibody sandwich ELISA was performed confirming the results. Considering the history of BCTV in relation to the agriculture industry of Utah, the discovery of novel hosts is cause for concern since this virus is adaptable and limits crop yield. The diagnosis of BCTV in native plants, such as the Four Corners potato and the big sagebrush, is pressing as it these local flora are unique to the region. The verification of BCTV in these novel hosts allows for the agriculture industry of Utah to prepare prevention methods including the monitoring of beet leafhoppers and increased integrated pest management strategies are needed to protect our local environment.

Utah Valley Flora Asteraceae Treatment

March 21, 2024 12:00 AM
Authors: Annika Wood. Mentors: Michael Rotter. Insitution: Utah Valley University. The Asteraceae is the most common plant family in Utah County, Utah. With over 79 genera and 238 species, it is also the most diverse family. Due to this substantial size and diversity, the genera of the Asteraceae have traditionally been placed into tribes. This confusing taxonomic situation has made the Asteraceae a noticeable but difficult group to identify. In particular the beginning botany students and non-technically trained citizens often can recognize the Asteraceae but have trouble distinguishing the many species. To help with this issue, we are creating a guide to the tribes and genera of the Asteraceae of Utah County. This key will provide a general view of which genera are connected and which are not. Knowing these patterns and differences could prove to be useful information for those who wish to know more about the fantastic plants that grow within Utah County. The course of action for creating these keys include research of the specimens in Utah Valley University's herbarium as well as other online herbariums. For the tribes we are specifically researching the distinct taxonomic classifications for each tribe individually as well as what connects them together. When those connections and distinctions are laid out in an understandable key, we will further create keys for each genera in the tribes. The end result we hope will be a useful and informative tool for identifying plants in the Asteraceae family.

Atmospheric Modeling of Brown Dwarfs With PICASO, SONORA, and JWST​

March 21, 2024 12:00 AM
Authors: Brayden Roberts, Joshua Lothringer, Denise Stephens. Mentors: Joshua Lothringer. Insitution: Utah Valley University. We present theoretical model fits to the spectra of two complex cloudy L-type brown dwarfs, VHS 1256b (L8) and J0624 (L5). Using the James Webb Space Telescope’s (JWST) Near Infrared Spectrograph (NIRSpec) and Mid-Infrared Instrument (MIRI), we get the complete spectra from 0.6-28 microns. We first fit the data using two forward model grids from the Sonora group to estimate the temperature and cloud properties of our object. Then, we apply a modified retrieval fitting code called PICASO, to make more complex fits to our observations. We conclude that forward models struggle to accurately fit our objects, while PICASO’s complex modeling can help us better understand what physics and chemistry could be lacking from our forward models.

Your Tooths Appetite for Hydroxyapatite

March 21, 2024 12:00 AM
Authors: Megan Wesenberg, Britain Jeppesen, Brianna McDaniel. Mentors: Jodie Lopez. Insitution: Utah Valley University. Abstract: Hydroxyapatite is a natural substance found in bones and the enamel of teeth. When hydroxyapatite is compromised, demineralization begins to occur which can eventually lead to cavities. There are many fluorides on the dental market, some having negative connotations associated with them. Hydroxyapatite products have been explored as an alternative for traditional fluorides to create similar effects without the potential for toxicity. Fluorosis is the main toxicity point addressed. Many patients are apprehensive of the use of fluorides due to possible overconsumption and uncontrolled levels within the local water supply. Because Hydroxyapatite is very biocompatible, access to it does not pose any toxic concerns. Using this alternate material allows these patients access to similar benefits in the oral cavity that fluoride would provide them while also being considerate of their personal beliefs and concerns. Caries are an extremely prevalent issue within the dental office, accounting for pain in patients and a majority of treatment done daily. Caries are very prevalent in the adult population. Hydroxyapatite is a useful substance in caries prevention, another similarity that it has in common with fluorides. Remineralization of these early carious lesions through the aid of hydroxyapatite leads to avoidance of more invasive treatment at a later date. A common use for fluoride is limiting sensitivity of teeth that can be felt from a variety of different stimulants within the oral cavity. Hydroxyapatite can also be used as a desensitizing agent. Hydroxyapatite enters dentin tubules and blocks them, limiting nerve stimulation within the dental pulp.

Establishing and Monitoring New Populations of an Endangered Plant (Phacelia argillacea) Over Several Years

March 21, 2024 12:00 AM
Authors: Tyler R Shaver, Lainey S Smith, Michael T Stevens. Mentors: Michael T Stevens. Insitution: Utah Valley University. Clay phacelia (Phacelia argillacea) was placed on the endangered plant list in 1978; at that time there were less than a dozen specimens alive at a single location on private land. This year, that same location, a steep hillside in Spanish Fork Canyon, had over 300 individuals. For the past four years, at suitable sites on public land nearby, we have been planting clay phacelia seeds and monitoring seedlings to understand the factors that will lead to successful establishment of this plant on public land. Following the planting of 32,400 seeds in 2020 and 13,500 in 2021, germinants that emerged were monitored and counted in the spring, summer, and fall each year between 2021 and 2023. In 2021, ten seedlings emerged, and of those six survived to produce seed. In 2022, 110 seedlings emerged, and of those 3 survived to produce seed. In 2023, 30 seedlings emerged, and of those four have survived as of the last monitoring trip. It is interesting to note that although emergence across the three years varied by as much as 11-fold, the number of plants that survived to reproduce was uniform and consistently low. The majority of seedlings that survived to produce seeds emerged in the late summer and fall. It is likely that monsoonal rains that occur in the late summer and fall make clay phacelia seed production more probable.

Remote sensing mineral identification: an intercomparison of Hyperspectral and multispectral imagery using open-source applications in the Marysvale Volcanic Field.

March 21, 2024 12:00 AM
Authors: Noah Christensen, Matt Olson. Mentors: Matt Olson. Insitution: Utah Valley University. Geologic maps serve as a valuable tool with diverse applications, one of which is resource exploration. Understanding available resources in America is beneficial for enhancing energy security, environmental sustainability, and economic growth. As political discourse rises, it is crucial to address national security concerns and head towards short- and long-term goals of energy independence. Traditional geologic mapping is characterized by time-intensive and high-cost fieldwork, but through multispectral and hyperspectral remotely sensed imagery it is possible to instantly map extensive areas using unique absorption patterns of minerals in the reflected spectral signature of the electromagnetic spectrum. This study explores an intercomparison of mineral mapping using Hyperion (EO-1), ASTER, and AVIRIS imagery in the Marysvale Volcanic Field, Utah. The satellites and high-altitude aircraft were chosen due to their varying number of spectral channels, spectral ranges, and spatial resolutions, allowing for a cross-analysis of their strengths, weaknesses, and overall capabilities. While previous studies have utilized AVIRIS and ASTER imagery within the Marysvale Volcanic Field, no prior research has explored a comparison of mineralogical maps using these sensors at a specific location. All analysis will be conducted through open-source applications to promote accessibility in future research and reproducibility of image generation through the sharing of R code. This research will enhance our comprehension of the necessary spectral and spatial resolutions for generating accurate mineral identification. Published geologic maps and in-situ field samples were used to validate the generated maps. We expect to find one of two outcomes: a strong contrast in classified mineralogy depending on the image source used, pointing towards a need for higher spatial and spectral resolutions to achieve accurate mapping, or minimal variation in classification, indicative of an unnecessary number of spectral bands. Developing accurate and accessible mineral mapping tools may be the next step in strengthening our knowledge of resource availability, without the need for rigorous traditional mapping methods.

Spatial Dynamics of Foliar Fungal Community Structure in the Pando Aspen Clone

March 21, 2024 12:00 AM
Authors: Austen Miller, Josh Leon, Geoffrey Zahn. Mentors: Geoffrey Zahn. Insitution: Utah Valley University. The Pando aspen grove in Central Utah is a remarkable organism comprising a clonal colony of Quaking Aspen (Populus tremuloides) in Fishlake National Forest. While Pando has garnered plenty of attention, the role of the fungal microbiome within this clonal system remains relatively uncharted. This research project delves into the endophytic and epiphytic fungal communities inhabiting the leaves of this giant single organism. The clonal nature of Pando means that plant genotype is consistent across a broad area, allowing us to test hypotheses about how plant-associated fungi disperse and associate with their hosts. In particular, we are testing whether proximity to a forest edge affects foliar fungal community structure. This research will enhance our understanding of how forest patch size and location contribute to plant-fungus community formation. These findings may also have implications with the potential to affect the health and resilience of this aspen clonal colony, which could in turn inform conservation strategies and forest management in the face of environmental challenges and the shifting ranges of aspens and fungal symbionts.

Host range testing and pathogenicity confirmation of potentially new blueberry fungus

March 21, 2024 12:00 AM
Authors: Kayla Knaperek, Megan Frisby, Geoffery Zahn, Alma Laney. Mentors: Alma Laney. Insitution: Utah Valley University. In February 2021, mycelial growth was found on blueberries, leading to the obtention of a fungal isolate using potato dextrose agar (PDA) after surface sterilization of the blueberries. The rapid growth of white mycelium and sclerotia was observed on the medium. Inoculation of basil plants with spores from surface-sterilized sclerotia in potato-dextrose broth resulted in necrosis and spreading in terminal buds, ultimately leading to plant death. Further isolation of the identical fungus from the diseased basil tissue confirmed its pathogenicity. Analysis of the isolate's ITS region sequencing revealed a 90% nucleotide identity to the closest relative, Botrytis cinerea. A project was initiated to further characterize the fungus, pathogenicity, life cycle, and morphological traits. The growth and development of the fungus were observed on V8, cornmeal, blueberry, and potato dextrose agars to characterize its nutritional requirements and growth patterns. Host range testing involved the systematic inoculation of various plant species (which were used as growth media), followed by careful symptom monitoring and photographed documentation. Additionally, Koch’s Postulates will be conducted to establish the causal relationship between fungal infection and the observed disease symptoms in the host plants. Seven days after inoculation, necrosis, wilting, and leaf discoloration were observed on select leaves of Lavender var. Oregano, suggesting possible infection by the blueberry fungus. Considering potential host plant immaturity during the initial inoculation, a re-inoculation is planned to ensure successful infection and to obtain conclusive results. Further investigation is warranted to confirm the pathogenic impact of the fungus on host plants.

Using Carbon and Nitrogen Stable Isotopes to Determine the Diet Composition of Ground Beetles (Coleoptera: Carabidae) in Provo Canyon, Utah

March 21, 2024 12:00 AM
Authors: Hayley Karns, Dylan Bryant, Noah Christensen, David Kirby, Sarah Marchant, Sarah Nicholas, Emmaline Saunders, Hannan Thomas, Nathan Smith, Dan Cavan, Daniele Ghiretti. Mentors: Alessandro Zanazzi. Insitution: Utah Valley University. Arthropods play a crucial role within terrestrial ecosystems by virtue of their substantial consumption of plant matter, their status as primary prey for various organisms, their function as key recyclers of organic material and nutrients in ecosystems, and their pivotal role as primary pollinators. Ground beetles (Coleoptera: Carabidae, or “Carabids”) represent a prominent and highly diverse taxonomic assemblage among arthropods inhabiting temperate forest floors. Despite their significance in these ecosystems, little is known about their trophic structure and level. These knowledge gaps primarily arise from the limitations associated with traditional investigative methods. For instance, the observation of feeding behavior in carabids proves challenging due to their diminutive size and predominantly nocturnal foraging habits. These uncertainties hinder the attainment of an understanding of community structure and trophic interactions both within the arthropod realm and with the vertebrates in their ecosystem. As a result, this research aims to gain a greater knowledge of the trophic structure of carabids using 15N/14N and 13C/12C isotopic analysis. Ground beetles were collected using pitfall traps placed near Big Springs Park (Provo Canyon, northern Utah) in September 2023. The traps were made with two plastic cups: a smaller cup was suspended inside a bigger cup. The smaller cup was partly filled with vinegar to attract the insects whereas the bigger cup was partly filled with monoethylene glycol, a compound that has been shown to preserve the insects without affecting their isotopic composition. Insect samples were collected over a period of four weeks. The beetles were then cleaned with a 2:1 mixture chloroform and methanol, dried, ground in an agate mortar and pestle, and placed in tin capsules for the isotopic analyses. Once the results are obtained, mixing model analysis will allow us to estimate the proportions of different food items based on the stable isotope ratios of consumers and food items and on known fractionations. This knowledge will contribute to a greater understanding of trophic structures and carbon pipelines in local ecosystems of northern Utah, and to a better prediction of ground beetle response to ongoing global warming and the associated changes in forest environments.

Survey of Blow Fly (Diptera: Calliphoridae) Species Across Utah and Salt Lake Counties

March 21, 2024 12:00 AM
Authors: Haylee Beck, Lauren Weidner, Carl E Hjelmen. Mentors: Carl E Hjelmen. Insitution: Utah Valley University. Blow flies, scientifically known as family Calliphoridae, are important primary colonizers of remains that play a crucial role in decomposition, making them useful in forensic investigations for estimating a time of colonization (TOC). Unfortunately, there is little information on the species and distributions of blow flies present in Utah. Our research seeks to address this issue through collection, preservation, and identification of these blow flies. To collect the specimens, traps baited with chicken liver were set up in various locations across Salt Lake and Utah Counties. While we have only collected from these two counties thus far, our intention is to gather flies across the state, spanning both rural and urban environments. We will then use standard metrics of diversity to compare the species makeup in each location. The results of our research offer significant insights into the biodiversity, distribution, and ecological functions of these flies and their development in different ecosystems. In addition, this research has far-reaching implications in the field of forensics. These findings will contribute significantly to our understanding of blow fly distribution and behavior, providing valuable information for forensic investigations in our region.

Production of Antibiotics in Soil Bacteria against ESKAPE Pathogens

March 21, 2024 12:00 AM
Authors: Cori Bailie, Arlene Majers, Katherine Judge. Mentors: Olga Kopp. Insitution: Utah Valley University. Ever since antibiotics have been in use, bacteria have been evolving to develop resistance against them. Each year, the number of people who die from previously treatable infections increases, showcasing the seriousness of this global issue. According to the CDC, facilities were overwhelmed during the 2020 pandemic, decreasing antibiotic production. By refocusing on discovering and producing new antibiotics, resistance can once again begin to be combatted. Bacteria found in soil samples were used to test for antibiotic production, using safe relatives to the ESKAPE pathogens. Starting with three master plates on Tryptic Soy Agar, 39 total bacterial colonies were studied. Colonies of interest were then PCR tested for DNA sequencing, and will be run through multiple different chemical tests for classification. Results showed that three different colonies displayed antibiotic activity. Colony 7 on master plate A produced antibiotics against Bacillus subtilis. Colonies 6 and 13 on master plate B, both produced antibiotics against Mycobacterium smegmatis, and Staphylococcus aureus. The results suggest that there are more soil bacteria to be discovered that may be able to produce antibiotics for common infections that are getting harder to treat. Further testing needs to be done to determine whether these colonies are safe for human therapy.

Do the differences in size between heteromorphic sex chromosomes influence organism longevity?

March 21, 2024 12:00 AM
Authors: Olivia Frary, Carl Hjelmen. Mentors: Carl Hjelmen. Insitution: Utah Valley University. Previous work has shown that there are significant differences in lifespan between sexes of the same species. Whether it is an XY or ZW system, the heterogametic sex dies earlier. There are thought to be many factors that contribute to the difference in lifespan between the sexes. This research aims to investigate this relationship further by digging deeper into the degree of differences between the heteromorphic sex chromosomes. We want to determine whether the degree of differentiation correlates with the lifespan differences in species. The comparison of total genome size between the sexes of multiple species are used to find the difference in the size of their sex chromosomes, which is then compared to longevity data on the species to determine a relationship. This genome size data comes from publicly available databases and literature. These comparisons are made using comparative phylogenetic methods in order to account for evolutionary relatedness utilizing a newly constructed phylogenetic tree. All analyses are completed in R and include PGLS (Phylogenetic Generalized Least Squares) and measures of phylogenetic signal (Pagel’s lambda, Blomberg’s K). We will discuss the results of the comparative phylogenetic analyses and any patterns seen regarding longevity.

Creating a universal framework for reconstructing phylogenies: Building trees at your fingertips using R

March 21, 2024 12:00 AM
Authors: Barbara Jetton, Carl E Hjelmen. Mentors: Carl E Hjelmen. Insitution: Utah Valley University. This project’s emphasis is the creation of an accessible and reusable tool to be used in broader scientific inquires of evolutionary relatedness. As of January 2023, GenBank contains 2.9 billion nucleotide sequences representing 504,000 distinct species. Despite this abundance of data, comprehensive and up to date phylogenies are lacking, impeding investigation into genetic histories and trait evolution. To address this problem, I am developing an open-source pipeline to expedite the construction of these evolutionary trees. I have a specific aim of creating a phylogeny for the order Diptera (flies) in order to investigate the evolution of the chromosome numbers for over 2500 species with chromosome count data on karyotype.org. I use R code, and the packages “reutils”, “ape”, and “seqinr”, to create reusable universal scripts which pull accession numbers from NCBI GenBank for each species based on the requested gene names. A second script was built to use the curated accession numbers to pull FASTA sequence data for each gene and write a multi-FASTA file for each gene, resulting in a comprehensive dataset necessary for alignment and phylogenetic tree construction. This effort will result in updated insights on the evolutionary history of Diptera related to chromosome numbers and can be used in further research in comparative biology. Additionally, these scripts can be used to investigate and reconstruct phylogenetic information for any species group with sequence data available on GenBank.

Identifying and photographing the instars of a parthenogenetic mayfly (Centroptilum trianguilfer; Ephemeroptera)

March 21, 2024 12:00 AM
Authors: Heidi Kipp, Annika Jones, . T. Heath Ogden. Mentors: T. Heath Ogden. Insitution: Utah Valley University. The purpose of this research is to identify and document mayfly instars from a parthenogenetic population of individuals being raised in the lab. Instars are the developmental stages of an arthropod between molts. Mayflies (and other non holometabolous insects) go through incomplete metamorphosis, moving from egg to nymph stages, and then to subimago (unique to mayflies), and imago (or adult). Previous research indicates the number of instars to be between 12-45 depending on the species, and the species we studied is estimated to have around 15. During each instar, mayflies shed their exoskeleton. The difference between each instar is vital to understanding the changes in gene expression and overall life cycle of a species. Instars have subtle differences, and identifying them will help further research into which genes are involved in producing specific morphological characteristics. Defining these structures, such as the forming of eyes or wings, are of particular interest in evolutionary phylogenetics. We raised Centroptilum trianguilfer (Ephemeroptera: Baetidae) in a controlled water tank, collecting and dating egg samples from mature imagos. Water is maintained with standard ion composition with 130-400 conductivity. Once the eggs hatched, we performed daily inspections and measurements using a 40x stereomicroscope microscope and Lumenera Infinity Analyze. We are using basic appearance, size and morphology) to separate instars. We will identify and photograph each instar. Samples from the different instars, subimagos, and adults will be acquired and used to generate transcriptome data. We propose that our research will aid in the investigation of mayfly evolution and their genetic mechanisms of control.

"Cognitive Insights into Madagascar Hissing Cockroaches: Memory, Learning, and Motivated Behavior"

March 21, 2024 12:00 AM
Authors: Brandon Barlow, Ethan Reese, Victoria Vazquez, Abbi Stark, Monica Sanchez, Alex Wilson, Rain Evans, Josef Becker, Amy Chevrier, James Taylor. Mentors: James Taylor. Insitution: Utah Valley University. This study focuses on memory and learning observed in Madasgascar Hissing Cockroaches (MHC). Research on insects can be used to inform our understanding of behavior and cognitive processes and add to our existing knowledge about the studied species. While there is existing literature focused on memory and learning in many insects, cockroaches are often chosen as a model due to their adaptability, resilience, and availability. Little research on MHC currently exists, especially in regard to memory and learning or drug seeking behaviors. This study uses a mixed model design, looking at arm choice percentages and latency to choose, between the control and experimental groups and comparing trials for each subject. In earlier trials, MHC demonstrated behavior that indicated they had learned not to fear light, a naturally aversive stimulus for cockroaches. Building on data collected in earlier trials, we developed a new protocol to evaluate the MHC associative learning behaviors and to test drug-seeking as a motivated behavior. Subjects are individually tested in a T-shaped apparatus in which they have to choose an arm to go into or are timed out. Each arm has a specific reward, either a sucrose and ethanol solution (experimental group) or plain sucrose solution (control group) in the left arm, and plain sucrose solution (experimental group) or water (control group) in the right arm. Each subject is run 2 days (6 trials per day) in these conditions, and then 3 days with the left arm lengthened to test if the subjects will seek their preferred reward even if it requires traveling a greater distance.

Characterization of an Acousto-optic Modulator for Implementation in a Q-switched Nd:YVO4 Laser

March 21, 2024 12:00 AM
Authors: Benjamin Holt. Mentors: York Young. Insitution: Utah Valley University. Our research group aims to create an optical system which generates tunable longwave infrared (LWIR) light as a tool for early detection of disease-indicating molecules in human tissue. This system begins with our newly constructed Nd:YVO4-type laser which generates between five and seven watts of continuous wave optical power in the near IR spectrum. This laser light will be used to drive the subsystem responsible for generating LWIR light: an optical parametric oscillator. In order for wavelength conversion to occur via a non-linear process, the near IR laser power serving as an optical drive must have an intensity on the order of tens of MW/cm2. To achieve such intensities the laser power must be concentrated into high intensity nanosecond length pulses by Q-switching the laser. Our method of Q-switching the Nd:YVO4 laser will be via an acousto-optic modulator in the laser cavity. My work to design the modulator’s kinematic mount, cooling system, and RF driver (as well as characterizing the performance of the modulator for use as a laser Q-switch) is described in this presentation.

Investigating the relationship between natural environment and drosophilid genome size.

March 21, 2024 12:00 AM
Authors: Samantha Curnow, Carl Hjelmen. Mentors: Carl Hjelmen. Insitution: Utah Valley University. The genome sizes of insects vary immensely; some insects have only 100 million base pairs, while others reach nearly 18 billion base pairs. Many selective pressures can have an influence on genomes, but we still lack a clear understanding of which selective pressures cause such variation in genome size even within well-studied groups. To determine the influence of environmental factors on this variation, we investigate the relationship between genome size and the average natural environment of 152 Drosophila species. The nature of the relationship between genome size and environmental factors is investigated using phylogenetic models and linear analyses such as phylogenetic generalized least squares analysis. We collected data from peer-reviewed papers and publicly available datasets, such as karyotype.org and WorldClim, to compare the genome size of each species to the temperature, altitude, humidity, etc. of their natural environments using linear phylogenetic models. The distribution of these species was also visualized using geographic and biological analysis packages in R, such as geodata, to extract environmental data of specified regions, and phytools in combination with caper to run phylogenetic comparative analyses.

Kynurenine Deamination and Synthesis of Xanthommatin

March 21, 2024 12:00 AM
Authors: Stephen Chamberland, Craig D. Thulin, Katherine Solorzano, Cassandra Beddes, Nathan Twitchell, Abram Caruso. Mentors: Steve Chamberland, Craig D. Thulin. Insitution: Utah Valley University. This investigative procedure is a continuation of an experiment done by UVU undergraduate student Kyra Banks and her mentor, UVU Professor of Chemistry Craig Thulin, to determine the structure of xanthommatin. Xanthommatin is one of the primary pigments found in monarch butterflies, and many other insects. Data obtained during the mass spectrometric analysis was observed to indicate a possible deamination of xanthommatin, however the quantity of deaminated xanthommatin observed was too small and so the structure could not be determined. The research investigates the precedented deamination of kynurenine as a model to develop a procedure for the deamination of 3-hydroxykynurenine, primarily due to the fact that it is cheaper in price to xanthommatin, as well as a chemical precursor that can be used for the synthesis of xanthommatin. The implications of this suggest that the deamination procedure developed for 3-hydroxykynurenine could be successfully replicated with xanthommatin. After the deamination procedure has been successfully repeated, xanthommatin will be synthesized using one of a few procedures developed by Kumar, A., et al1. Once a sufficient sample of xanthommatin has been successfully synthesized, the deamination procedure will be repeated on samples of xanthommatin to determine the structure of deaminated xanthommatin using NMR spectroscopy. The research conducted here is beneficial not only because it is a continuation of other UVU student’s and staff’s work, but also to those whose work is in the animal biology realm of science. The information that will be extracted from this research will spearhead new research pathways and questions to be explored.Xanthommatin-Based Electrochromic Displays Inspired by Nature (acs.org)Biomimetic Colorants and Coatings Designed with Cephalopod-Inspired Nanocomposites (acs.org)

Groundwater and Surface Water Interactions on the Hobble Creek Watershed

March 21, 2024 12:00 AM
Authors: Roshan Abraham, Parker Jorgenson. Mentors: Daren Nelson. Insitution: Utah Valley University. Hobble Creek is an essential and valuable stream that the City of Springville and other local entities utilize for their water supply. However, the stream is also vital to the local environment and ecosystem since it one of the main streams that the June Sucker (an endangered endemic fish) spawn in each year. Numerous environmental projects and studies have been done in the lower section of Hobble Creek due to this importance. There are many springs in the region that provide water to the stream but there is a lack of consistent monitoring of the flow throughout the stream to determine how the stream is interacting with groundwater sources. Groundwater models completed by the United States Geological Survey in 2005 for the Utah Lake Watershed suggest locations where groundwater interactions should be either discharging or recharging the local aquifer. However, more recent data has suggested that many of these water interaction maps created by the USGS model are inaccurate. Using a water budget analysis, streamflow in the creek is being measured at 10 sites throughout the Hobble Creek Watershed to determine how the flow in the creek changes as it flows towards Utah Lake. Data gathered from this and previous studies show the zones of discharge and recharge shift seasonally and are not as consistent as the USGS model suggests. Recent data does confirm that Hobble Creek is gaining flow upstream and losing flow downstream, but there are portions of the river that also may be perched (do not interact with the groundwater). Further measurements and continued site investigation will be necessary to help us better manage this vital water resource.

Groundwater and Surface Water Interactions in the Provo River Watershed

March 21, 2024 12:00 AM
Authors: Feyan Hoffman, Roshan Abraham, Christine Fowles, Abi Conger, Scott Jenson. Mentors: Daren Nelson. Insitution: Utah Valley University. One of the least understood part of our local water budget is the interaction of groundwater into our lakes and streams. Groundwater models completed by the United States Geological Survey in 2005 for the Utah Lake Watershed suggest zones where groundwater interactions should be either discharging or recharging into our local streams, wetlands, and other water bodies. However, more recent data has suggested that many of these water interaction maps created by the model are inaccurate, and we need a better understanding of how groundwater interacts with these water bodies. Using a water budget analysis, groundwater and surface water interactions are measured by collecting discharge measurements using flow meters at different cross sections along the Provo River and its tributaries. A gaining or losing stream may swap depending on seasonal changes, such as a particular water year being dry or wet–a stream that is typically gaining may be recharging the aquifer during a dry year. The USGS model is a good basic representation of interactions between groundwater and surface water. Our data suggests that due to highly variable water levels of Utah Lake and local groundwater levels, some discharge and recharge zones in the Provo River subbasin are likely to change over time. In addition, some sections of the river may be perched and not interact with the groundwater system at all. In order for us to better manage these important resources, further analysis of flow behaviors in the Provo River and its tributaries need to be completed. This study is part of a larger study that includes adding two new permanent flow gauging stations to provide a long term record that will enable water managers to better decipher why the groundwater and surface interactions are not fitting the USGS model.

Sound Levels on the UVU Pedestrian Bridge

March 21, 2024 12:00 AM
Authors: Jacob Sampson, Joshua Goates, Brian Patchett, Abolfazl Amin, Bonnie Andersen. Mentors: Bonnie Andersen. Insitution: Utah Valley University. Noise from an interstate can reach extreme levels. The OSHA limit for when sound becomes dangerous for the health of individuals is 85 dB. At UVU there is a pedestrian bridge that goes over I-15 and the students and other pedestrians that use this bridge are exposed to sound levels that could be potentially dangerous for their hearing, along with other adverse health effects due to sound on a regular basis. Sound levels have been measured on the bridge using two different measuring devices. The first being an Extech noise dosimeter, that was worn on the shoulder of the measurer as they walked across the bridge. The second instrument used was a Larson David sound level meter. That was placed on several places along the length of the bridge at set to record for several minutes to get the sound levels at those places. Sound levels that have been recorded have ranged form 79 dB to 102 dB. The OSHA limit for occupational exposure at 100 dB is two hours while for non-occupational exposure it is only 15 minutes. This research seeks to better understand and quantify the noise exposure pedestrians experience on the bridge. Also this research hopes to find out what facts influence the sound levels on the bridge and we hope to compare our experimental results to an ANSYS model of the bridge to confirm our findings.

Mechanical to Electrical Energy Conversion of a Door Swing

March 21, 2024 12:00 AM
Authors: Spencer Brown, Shawn Pepper, Carson Draughon, Ulises Thornock, Dustin Shipp. Mentors: Dustin Shipp. Insitution: Utah Valley University. In the shadow of the 2021-2023 global energy crisis, the need for alternative sources of energy has reached an all time high. To combat this, we have developed a prototype method of harvesting kinetic energy from a door swing, converting that energy into electricity, and storing it inside a battery for future use. Our experiments have demonstrated the capability to use the energy from one door to charge a cellular phone, but we believe this method could be applied on a much larger scale. In this poster we will discuss the method, design, and measurements from our device, as well as possible future applications.

Droplet Breakup: 'It's Not You, It's My Vibe' – Achieving Uniformity in Free Jets Through Vibrational Tactics

March 21, 2024 12:00 AM
Authors: Miranda Rose, Daniel James. Mentors: Daniel James. Insitution: Utah Valley University. In this study, we investigated mechanical vibrations as a mechanism for creating uniform droplets in a free liquid jet. A stream of liquid naturally breaks into irregular droplets as it falls due to the Plateau-Rayleigh instability. In this work, droplet break-up was triggered by utilizing a motor with Arduino-based frequency control and stroboscopic visualization. The project achieved uniform droplet sizes for multiple liquids of different viscosities. Notably, this method demonstrated the creation of Dippin Dots-like confections at a STEM fair, engaging learners in the principles of physics and engineering. Future work will focus on the original goal of utilizing acoustics for droplet formation, aiming to refine the process and expand its industrial applicability.

Assessing Genomic Diversity Via whole genome resequencing in bean cultivars (Phaseolus L. spp.) from Brazil, a putative secondary center of diversity.

March 21, 2024 12:00 AM
Authors: Kaycie A Melville, Ashley N Egan, Yu Ya Liang. Mentors: Ashley N. Egan. Insitution: Utah Valley University. Phaseolus vulgaris (common bean) and P. lunatus (lima bean) provide protein and nutrition for millions of people across the globe. Native to the New World, these cultivated species present unique histories of multiple and independent domestications in Mesoamerica and the Andes. Brazil is the leading producer globally of these beans. With both wild and cultivated landraces present, Brazil may act as a secondary center of domestication for these two economically significant leguminous crops. Brazil's indigenous populations’ cultivation efforts may include both wild and cultivated germplasm, potentially contributing to the continued domestication of these crops to create novel shapes, sizes, and colors that are grown and hand-selected by the natives in these regions. To investigate Brazil as a secondary domestication center of beans, this research addresses several key questions: Are signals of past and current domestication processes discernible in current Brazilian cultivars of these species? Can we unravel the relationships between these Brazilian crops and their primary centers of domestication? What genetic differences are underpinning domestication? How diverse are Brazilian cultivars? How do they relate to wild and related material and species? This work focuses on 48 accessions of P. lunatus and P. vulgaris from Brazil, including wild material and two accessions from two related species, P. polystachios and P. filiformis from North America. Whole genomes were resequenced using Illumina sequencing and compared against the published genome of Phaseolus vulgaris to identify SNPs in each accession. Whole chloroplast genomes were also assembled and compared. Phylogenetic analyses, network analyses, population structure, and tests for neutral and positively selected mutations were conducted, revealing distinct clades for both P. vulgaris and P. lunatus with clear links to their Andean and Mesoamerican origins. Genetic diversity was assessed both within (cultivated vs wild) and between P. vulgaris and P. lunatus, demonstrating significant variations, not only between species but also within each species, suggesting the influence of diverse selection pressures and local adaptation during the domestication process. McDonald-Kreitman tests analyze and locate possible domestication genes from the library of SNP’s between outgroups (Phaseolus filiformis and Phaseolus polystachios) and between species. Overall, this research advances our understanding of the intricate process of domestication in P. vulgaris and P. lunatus, highlighting potential ongoing domestication in Brazilian cultivars, unraveling their genetic relationships, and shedding light on the complex interplay of genes that underpin domestication and diversity. These findings contribute to the broader understanding of crop evolution and have practical implications for crop improvement and conservation.