Fine Arts

Authors: Ellie P. Evans, Mmadili Ilozumba, Sheetal Hardikar, Neli Ulrich, Jennifer Ose. Mentors: Mmadili Ilozumba. Insitution: Utah Valley University. Cachexia is a complex syndrome characterized by severe weight loss and muscle wasting. It is also a prevalent complication in various chronic diseases, including cancer and acquired immunodeficiency syndrome. Recent research has underscored the pivotal role of the gut microbiome in systemic well-being. Furthermore, it has been shown that cancer patients suffering from cachexia have unique bacteria composition in the gut compared to that of non-cachectic patients. In the absence of stool samples, there are a few metabolites in the circulatory system that can be directly linked to gut microbiota. Examples include bile acids, and short chain fatty acids as metabolites produced by specific Phylum. The presence of these gut microbiota derived metabolites (GMDM) in the circulation indicate a compromise in the integrity of tight junctions in the intestines. These results prompted an investigation of GMDM as an indicator of gut permeability. Our hypothesis is that an increased concentration of GMDM in the circulation is linked to gut permeability, leading to the development of cachexia in patients with colorectal cancer.  This literature review provides a comprehensive analysis of the relationship between gut permeability and cachexia, seeking to identify which GMDMs are the most accurate indicators of specific bacteria linked to gut permeability, and subsequently, are accurate projectors of cachexia.  Acetic acid, a short chain fatty acid (SCFA) is a GMDM detected in the plasma of patients with gut permeability. Acetic acid makes up about 60% of the SCFAs produced by a healthy gut flora and 90% of it is produced by the genus Bifidobacterium. Our literature review will cover how closely a measurement of acetic acid and other GMDMs measured in plasma can be linked to gut permeability and to Bifidobacterium levels in the gut. Similarly, Indole is a GMDM produced by Escherichia coli and other indole-producing bacteria. Indole isn’t produced naturally anywhere else in the body. When it is naturally absorbed into the liver through the portal vein, it’s mostly converted into indoxyl sulfate. The heightened presence indole in plasma in sulfate-free forms could indicate that indole is being absorbed through holes in the tight junctions of the intestines, rather than through the portal vein. This information would allow cohorts that have collected plasma samples in the past to measure gut permeability by proxy, even if they’re unable to investigate the gut microbiota and metabolites present from stool samples. These connections found could further help us to use-already collected samples to find accurate biomarkers for early cachexia development in cancer patients. If an oral presentation is unavailable, a poster presentation would be great.

Authors: Isabella Scalise. Mentors: Conan Kinsey. Insitution: University of Utah.

Authors: Allison Stevens, Gennie Parkman. Mentors: Gennie Parkman. Insitution: Weber State University. The principal genes that are responsible for the conversion of benign nevi, or pigmented lesions, to cancerous melanomas have been discovered and validated. However, we are still identifying the additional genetic alterations that are responsible for the migration and invasion of melanoma cells and ultimately metastasis of these melanomas as that is where treatment needs remain. (Davies, et al., 2002)One gene that has been identified to potentially play a role in the metastasis of melanoma is thymocyte selection associated high mobility group box, or TOX. Based on one study, the levels of TOX gene expression were found to be elevated in melanoma brain metastases versus extracranial metastases (Bierman et al., 2022). These findings have led us to hypothesize that TOX plays a role in the migration and invasion of melanoma into the brain. Preliminary work has been completed to clone our gene of interest, TOX, into an expression vector and transduce mammalian melanoma cells to evaluate their effects on melanoma cell rate growth and migration. These experiments will identify the effect of TOX on the initiation and progression of melanoma cells through in vitro melanoma cell culture and experimentation before potentially progressing to well-validated mouse models of melanoma.

Authors: Matthew Stecher, Terungwa Iorkula, Matt A. Peterson. Mentors: Matt A. Peterson. Insitution: Brigham Young University. Tropomyosin Receptor Kinase (TRK) inhibition has become an effective therapeutic approach for treatment of a variety of cancers including soft tissue sarcoma, pilocytic astrocytoma, non-small cell lung cancers, colorectal cancers, glioblastomas, pediatric gliomas, papillary thyroid cancers, and secretory breast carcinoma. Despite the successful use of first-generation TRK inhibitors Larotrectinib and Entrectinib which have shown significant therapeutic response in patients, acquired resistance to these drugs has emerged and is creating serious barriers to maintaining long-term therapeutic efficacy. A common mechanism for acquired resistance is associated with the emergence of mutations. Hence, mutation-induced drug resistance continues to be a major challenge for both first- and second-generation TRK inhibitors. Here, we present the design of novel macrocyclic 3,5-diaminated pyrazolo[1,5-a] pyrimidine ligands with high potential for combating drug-resistant disease.

Authors: . Mentors: Jared Nielsen. Insitution: Brigham Young University. Objective: This study examines the role of positive reframing as a strategy to mitigate suicidality and enhance positive emotions. Background: Emotional regulation difficulties have been identified in numerous psychiatric populations, potentially leading to stress, incarceration, and tragically, suicide. Amidst the rising rates of suicidality among at-risk populations, this study aims to investigate the pivotal behavioral factors influencing suicidal thoughts and actions in autistic and socially anxious populations. Methods: Our investigation employed a longitudinal ecological momentary assessment study to explore the impact of various emotional regulation skills on positive emotion, negative emotion, and suicidality. We recruited 95 participants, 72 of which met criteria for inclusion, to answer daily surveys administered through a smartphone app that tracked emotional regulation use, positive emotions, negative emotions, and suicidal tendencies over time. Results: Our results indicate that for the social anxiety group, enjoying the moment significantly affects positive emotions (E=2.14, p<.05), but this effect is not observed in the autism group. Furthermore, both groups demonstrated that accepting feelings (E=4.40, p<.001; E=2.63, p<.01) and acknowledging that feelings don’t last (E=1.69, p<.05; E=3.13, p<.001) significantly increased daily positive emotions. Conversely, hiding feelings significantly decreased daily positive emotions in the social anxiety group (E=-1.76, p<.05). Self-blame increased negative emotions in both groups by, on average, four points. Regarding the reduction of daily average suicide composite scores, accepting feelings emerged as a significant factor for both groups (E=-1.88, p<.01; E=-2.15, p<.001). On the other hand, using self-blame significantly increased average suicide composite scores for social anxiety, mirroring its effect on negative emotions (E=1.42, p<.01). Conclusion: Positively reframing one's emotions is associated with increased daily positive feelings and reduced daily suicidal tendencies. This research sheds light on the complex interplay between emotional regulation strategies and their impact on mental health and emotions in these at-risk populations.

Authors: Robert Macdonald. Mentors: Troy Munro. Insitution: Brigham Young University. Due to the recent developments in the precision of small scale 3-D printing, the use of complex multi-dimensional heating geometries is now very feasible in microfluidics. Small channels can be filled with liquid metal and can become a heat source by passing an electrical current through the liquid metal. There is a desire for the creation of isothermal areas within microfluidic chips for processes such as polymerase chain reaction and melt curve analysis of DNA to detect mutations. One difficulty of designing isothermal areas in microfluidics is the complexity of the geometries involved and the precision needed to be useful. These two problems were addressed by using a finite element software COMSOL to simulate the microfluidic chip to aid in design iteration. Quick evaluation of the performance of different heating geometries in COMSOL allows for faster evolution of our design. Using these principles, a novel microfluidic chip design was developed with a simulated isothermal volume of 5 microliters and a temperature variation of <0.1 degrees Celsius.

Authors: Tanner Frahm, Eugene Kwan, Ravi Ranjan. Mentors: Ravi Ranjan. Insitution: University of Utah. Introduction:Stereotactic body radiation therapy (SBRT) is a non-invasive treatment for ventricular tachycardia (VT) that acts as a promising alternative to the current gold-standard treatment, catheter ablation. Preliminary clinical successes indicate the promise of SBRT while subsequent animal studies suggest that the mechanism of SBRT is novel and unknown. We propose SBRT may alter Connexin-43 (Cx43) gap-junction distribution in cardiac myocyte walls, affecting electrical conduction and reducing VT episodes. A large animal model was used to evaluate Cx43 changes post-SBRT, aiming to elucidate its mechanism of action. Understanding this could expand SBRT's use in arrhythmia management and explore myocyte reprogramming.Methods:A large animal model (n=3) underwent cardiac SBRT on portions of their heart. Each animal provided control, untreated tissue, and treated tissues. Post-treatment, the hearts were excised, perfused, and preserved. Transmural sections were processed for histological and confocal microscopy analyses to assess scarring and Cx43 distribution. An algorithm for semi-automatic myocyte segmentation and Cx43 distribution analysis was developed and used. Two-sample student t-tests were conducted to compare Cx43 distribution between treated and control regions. All image and statistical analysis were performed in MATLAB.Results/Discussion:Preliminary results for the three preliminary animals indicate that treated regions of the myocardium had statistically significant differences in Cx43 distribution (p-values < 0.05). Treated values (mean  standard deviation) were 48%  25%, 62%  26%, and 64%  17% of the distribution of Cx43 on the longitudinal ends while the untreated were 42%  30%, 44%  25% and 26% 16%. The proportion of Cx43 distributed along the longitudinal axis (the short ends of the myocyte) increased in all three animals. The results build on the findings of previous animal studies which suggested that the scarring like ablation was not the mechanism, but that Cx43 or other cellular components could be changing in response to therapy. Conclusions:The findings support the hypothesis that Cx43 distribution changes in response to SBRT indicating that the radiation could be inducing remodeling at the cellular level. Evidence of non-invasive cardiac reprogramming provides basic science credence to the preliminary and ongoing clinical studies evaluating the treatment in patients. The findings further inform and motivate more animal studies and analysis of changes to other cellular components.

Authors: Natalie Welsh, Lauren Brooks, Skyler Belka. Mentors: Natalie Welsh, Lauren Books. Insitution: Utah Valley University. When bacteria gain resistance to antimicrobial substances, they become a public health threat due to an inability to treat infections and control outbreaks. Staphylococcus aureus is a common element of the human microbiome that is widely associated with antibiotic resistance. While MRSA (or methicillin-resistant S. aureus) has long been prevalent, new types of antibiotic resistance, including VRSA (or vancomycin-resistant S. aureus), have become an increasing threat in recent years. This research aims to collect samples and use polymerase chain reaction (PCR) to screen for genes associated with resistance to common antibiotics, such as erythromycin, tetracycline, methicillin, and vancomycin. Molecular biology techniques are used to test various resistant genes. First, sample collection uses FloqSwabs dipped in phosphate-buffered saline and swabbed on various locations on the UVU campus (e.g., door handles/knobs, vending machines, elevators, stair rails, water fountains, cash registers and keypads, countertops). Then, DNA from collected swabs was extracted using the DNeasy PowerSoil Kit. PCR was used to amplify the 16S rRNA gene to act as a positive control and confirm the methodology captured bacteria. Once confirmed, we also examined the samples for various antibiotic-resistant genes (e.g., ErmA, ErmC, TetM, TetT, etc.). Lastly, amplified DNA is visualized using electrophoresis gels, observing under UV light to verify amplification. Understanding the genes found on campus and where they are most prevalent will help elucidate the risks to students on UVU’s campus, providing valuable public health knowledge.

Authors: Annika Wood. Mentors: Michael Rotter. Insitution: Utah Valley University. The Asteraceae is the most common plant family in Utah County, Utah. With over 79 genera and 238 species, it is also the most diverse family. Due to this substantial size and diversity, the genera of the Asteraceae have traditionally been placed into tribes. This confusing taxonomic situation has made the Asteraceae a noticeable but difficult group to identify. In particular the beginning botany students and non-technically trained citizens often can recognize the Asteraceae but have trouble distinguishing the many species. To help with this issue, we are creating a guide to the tribes and genera of the Asteraceae of Utah County. This key will provide a general view of which genera are connected and which are not. Knowing these patterns and differences could prove to be useful information for those who wish to know more about the fantastic plants that grow within Utah County. The course of action for creating these keys include research of the specimens in Utah Valley University's herbarium as well as other online herbariums. For the tribes we are specifically researching the distinct taxonomic classifications for each tribe individually as well as what connects them together. When those connections and distinctions are laid out in an understandable key, we will further create keys for each genera in the tribes. The end result we hope will be a useful and informative tool for identifying plants in the Asteraceae family.

Authors: Brayden Roberts, Joshua Lothringer, Denise Stephens. Mentors: Joshua Lothringer. Insitution: Utah Valley University. We present theoretical model fits to the spectra of two complex cloudy L-type brown dwarfs, VHS 1256b (L8) and J0624 (L5). Using the James Webb Space Telescope’s (JWST) Near Infrared Spectrograph (NIRSpec) and Mid-Infrared Instrument (MIRI), we get the complete spectra from 0.6-28 microns. We first fit the data using two forward model grids from the Sonora group to estimate the temperature and cloud properties of our object. Then, we apply a modified retrieval fitting code called PICASO, to make more complex fits to our observations. We conclude that forward models struggle to accurately fit our objects, while PICASO’s complex modeling can help us better understand what physics and chemistry could be lacking from our forward models.

Authors: Travis Christensen, Geoff Zahn, Alma Laney. Mentors: Alma Laney. Insitution: Utah Valley University. In July 2022, green cabbage plants (Brassica oleracea) were observed with severe viral symptoms in a local community garden in Utah County. These plants were observed to be covered with cabbage aphids (Brevicoryne brassicae). Symptoms observed on the cabbage plants included yellowing, necrotic lesions, and brittle necrosis of the leaves. These symptoms have been found to be transmissible by the cabbage aphid to broccoli, cabbage, and kale, although symptoms are delayed in kale. Work is being done to find other transmissible plants within the brassica family. Sequencing of RNA yielded no results, however, amplification of viral DNA via rolling circle amplification (RCA) has yielded positive results. A sample obtained through maintenance of the virus and cabbage aphid is currently being subjected to RNAseq using the NovaSeq Illumina platform. Through several virological methods we are able to conclude the presence of a putative geminivirus transmissible through cabbage aphid in our affected plants. To our knowledge, this is the first report of an aphid-transmissible DNA virus of brassicas in Utah. Further work is being done to further identify, classify, and build the genome of this specific virus using Illumina sequencing.

Authors: Ezra Wylie, Merrill Halling. Mentors: Merrill Halling. Insitution: Utah Valley University. Various parts of Cercocarpus ledifolius, or curled leaf mountain mahogany, was used for medicinal purposes by Native Americans for a wide variety of uses including tuberculosis, syphilitic sores, and venereal diseases. Symbiotic microorganisms, called endophytes, often produce these compounds for defense of the tree. Novel compounds have been found in plants which have demonstrated antibiotic, antifungal, or other biological activities. Endophytes were grown from various parts of the tree, including roots, stems, branches, and leaves. The bioactive compounds were isolated and identified using chromatographic and spectroscopic methods.

Authors: Megan Wesenberg, Britain Jeppesen, Brianna McDaniel. Mentors: Jodie Lopez. Insitution: Utah Valley University. Abstract: Hydroxyapatite is a natural substance found in bones and the enamel of teeth. When hydroxyapatite is compromised, demineralization begins to occur which can eventually lead to cavities. There are many fluorides on the dental market, some having negative connotations associated with them. Hydroxyapatite products have been explored as an alternative for traditional fluorides to create similar effects without the potential for toxicity. Fluorosis is the main toxicity point addressed. Many patients are apprehensive of the use of fluorides due to possible overconsumption and uncontrolled levels within the local water supply. Because Hydroxyapatite is very biocompatible, access to it does not pose any toxic concerns. Using this alternate material allows these patients access to similar benefits in the oral cavity that fluoride would provide them while also being considerate of their personal beliefs and concerns. Caries are an extremely prevalent issue within the dental office, accounting for pain in patients and a majority of treatment done daily. Caries are very prevalent in the adult population. Hydroxyapatite is a useful substance in caries prevention, another similarity that it has in common with fluorides. Remineralization of these early carious lesions through the aid of hydroxyapatite leads to avoidance of more invasive treatment at a later date. A common use for fluoride is limiting sensitivity of teeth that can be felt from a variety of different stimulants within the oral cavity. Hydroxyapatite can also be used as a desensitizing agent. Hydroxyapatite enters dentin tubules and blocks them, limiting nerve stimulation within the dental pulp.

Authors: Tyler R Shaver, Lainey S Smith, Michael T Stevens. Mentors: Michael T Stevens. Insitution: Utah Valley University. Clay phacelia (Phacelia argillacea) was placed on the endangered plant list in 1978; at that time there were less than a dozen specimens alive at a single location on private land. This year, that same location, a steep hillside in Spanish Fork Canyon, had over 300 individuals. For the past four years, at suitable sites on public land nearby, we have been planting clay phacelia seeds and monitoring seedlings to understand the factors that will lead to successful establishment of this plant on public land. Following the planting of 32,400 seeds in 2020 and 13,500 in 2021, germinants that emerged were monitored and counted in the spring, summer, and fall each year between 2021 and 2023. In 2021, ten seedlings emerged, and of those six survived to produce seed. In 2022, 110 seedlings emerged, and of those 3 survived to produce seed. In 2023, 30 seedlings emerged, and of those four have survived as of the last monitoring trip. It is interesting to note that although emergence across the three years varied by as much as 11-fold, the number of plants that survived to reproduce was uniform and consistently low. The majority of seedlings that survived to produce seeds emerged in the late summer and fall. It is likely that monsoonal rains that occur in the late summer and fall make clay phacelia seed production more probable.

Authors: Noah Christensen, Matt Olson. Mentors: Matt Olson. Insitution: Utah Valley University. Geologic maps serve as a valuable tool with diverse applications, one of which is resource exploration. Understanding available resources in America is beneficial for enhancing energy security, environmental sustainability, and economic growth. As political discourse rises, it is crucial to address national security concerns and head towards short- and long-term goals of energy independence. Traditional geologic mapping is characterized by time-intensive and high-cost fieldwork, but through multispectral and hyperspectral remotely sensed imagery it is possible to instantly map extensive areas using unique absorption patterns of minerals in the reflected spectral signature of the electromagnetic spectrum. This study explores an intercomparison of mineral mapping using Hyperion (EO-1), ASTER, and AVIRIS imagery in the Marysvale Volcanic Field, Utah. The satellites and high-altitude aircraft were chosen due to their varying number of spectral channels, spectral ranges, and spatial resolutions, allowing for a cross-analysis of their strengths, weaknesses, and overall capabilities. While previous studies have utilized AVIRIS and ASTER imagery within the Marysvale Volcanic Field, no prior research has explored a comparison of mineralogical maps using these sensors at a specific location. All analysis will be conducted through open-source applications to promote accessibility in future research and reproducibility of image generation through the sharing of R code. This research will enhance our comprehension of the necessary spectral and spatial resolutions for generating accurate mineral identification. Published geologic maps and in-situ field samples were used to validate the generated maps. We expect to find one of two outcomes: a strong contrast in classified mineralogy depending on the image source used, pointing towards a need for higher spatial and spectral resolutions to achieve accurate mapping, or minimal variation in classification, indicative of an unnecessary number of spectral bands. Developing accurate and accessible mineral mapping tools may be the next step in strengthening our knowledge of resource availability, without the need for rigorous traditional mapping methods.

Authors: Austen Miller, Josh Leon, Geoffrey Zahn. Mentors: Geoffrey Zahn. Insitution: Utah Valley University. The Pando aspen grove in Central Utah is a remarkable organism comprising a clonal colony of Quaking Aspen (Populus tremuloides) in Fishlake National Forest. While Pando has garnered plenty of attention, the role of the fungal microbiome within this clonal system remains relatively uncharted. This research project delves into the endophytic and epiphytic fungal communities inhabiting the leaves of this giant single organism. The clonal nature of Pando means that plant genotype is consistent across a broad area, allowing us to test hypotheses about how plant-associated fungi disperse and associate with their hosts. In particular, we are testing whether proximity to a forest edge affects foliar fungal community structure. This research will enhance our understanding of how forest patch size and location contribute to plant-fungus community formation. These findings may also have implications with the potential to affect the health and resilience of this aspen clonal colony, which could in turn inform conservation strategies and forest management in the face of environmental challenges and the shifting ranges of aspens and fungal symbionts.

Authors: Kayla Knaperek, Megan Frisby, Geoffery Zahn, Alma Laney. Mentors: Alma Laney. Insitution: Utah Valley University. In February 2021, mycelial growth was found on blueberries, leading to the obtention of a fungal isolate using potato dextrose agar (PDA) after surface sterilization of the blueberries. The rapid growth of white mycelium and sclerotia was observed on the medium. Inoculation of basil plants with spores from surface-sterilized sclerotia in potato-dextrose broth resulted in necrosis and spreading in terminal buds, ultimately leading to plant death. Further isolation of the identical fungus from the diseased basil tissue confirmed its pathogenicity. Analysis of the isolate's ITS region sequencing revealed a 90% nucleotide identity to the closest relative, Botrytis cinerea. A project was initiated to further characterize the fungus, pathogenicity, life cycle, and morphological traits. The growth and development of the fungus were observed on V8, cornmeal, blueberry, and potato dextrose agars to characterize its nutritional requirements and growth patterns. Host range testing involved the systematic inoculation of various plant species (which were used as growth media), followed by careful symptom monitoring and photographed documentation. Additionally, Koch’s Postulates will be conducted to establish the causal relationship between fungal infection and the observed disease symptoms in the host plants. Seven days after inoculation, necrosis, wilting, and leaf discoloration were observed on select leaves of Lavender var. Oregano, suggesting possible infection by the blueberry fungus. Considering potential host plant immaturity during the initial inoculation, a re-inoculation is planned to ensure successful infection and to obtain conclusive results. Further investigation is warranted to confirm the pathogenic impact of the fungus on host plants.

Authors: Hayley Karns, Dylan Bryant, Noah Christensen, David Kirby, Sarah Marchant, Sarah Nicholas, Emmaline Saunders, Hannan Thomas, Nathan Smith, Dan Cavan, Daniele Ghiretti. Mentors: Alessandro Zanazzi. Insitution: Utah Valley University. Arthropods play a crucial role within terrestrial ecosystems by virtue of their substantial consumption of plant matter, their status as primary prey for various organisms, their function as key recyclers of organic material and nutrients in ecosystems, and their pivotal role as primary pollinators. Ground beetles (Coleoptera: Carabidae, or “Carabids”) represent a prominent and highly diverse taxonomic assemblage among arthropods inhabiting temperate forest floors. Despite their significance in these ecosystems, little is known about their trophic structure and level. These knowledge gaps primarily arise from the limitations associated with traditional investigative methods. For instance, the observation of feeding behavior in carabids proves challenging due to their diminutive size and predominantly nocturnal foraging habits. These uncertainties hinder the attainment of an understanding of community structure and trophic interactions both within the arthropod realm and with the vertebrates in their ecosystem. As a result, this research aims to gain a greater knowledge of the trophic structure of carabids using 15N/14N and 13C/12C isotopic analysis. Ground beetles were collected using pitfall traps placed near Big Springs Park (Provo Canyon, northern Utah) in September 2023. The traps were made with two plastic cups: a smaller cup was suspended inside a bigger cup. The smaller cup was partly filled with vinegar to attract the insects whereas the bigger cup was partly filled with monoethylene glycol, a compound that has been shown to preserve the insects without affecting their isotopic composition. Insect samples were collected over a period of four weeks. The beetles were then cleaned with a 2:1 mixture chloroform and methanol, dried, ground in an agate mortar and pestle, and placed in tin capsules for the isotopic analyses. Once the results are obtained, mixing model analysis will allow us to estimate the proportions of different food items based on the stable isotope ratios of consumers and food items and on known fractionations. This knowledge will contribute to a greater understanding of trophic structures and carbon pipelines in local ecosystems of northern Utah, and to a better prediction of ground beetle response to ongoing global warming and the associated changes in forest environments.

Authors: Cori Bailie, Arlene Majers, Katherine Judge. Mentors: Olga Kopp. Insitution: Utah Valley University. Ever since antibiotics have been in use, bacteria have been evolving to develop resistance against them. Each year, the number of people who die from previously treatable infections increases, showcasing the seriousness of this global issue. According to the CDC, facilities were overwhelmed during the 2020 pandemic, decreasing antibiotic production. By refocusing on discovering and producing new antibiotics, resistance can once again begin to be combatted. Bacteria found in soil samples were used to test for antibiotic production, using safe relatives to the ESKAPE pathogens. Starting with three master plates on Tryptic Soy Agar, 39 total bacterial colonies were studied. Colonies of interest were then PCR tested for DNA sequencing, and will be run through multiple different chemical tests for classification. Results showed that three different colonies displayed antibiotic activity. Colony 7 on master plate A produced antibiotics against Bacillus subtilis. Colonies 6 and 13 on master plate B, both produced antibiotics against Mycobacterium smegmatis, and Staphylococcus aureus. The results suggest that there are more soil bacteria to be discovered that may be able to produce antibiotics for common infections that are getting harder to treat. Further testing needs to be done to determine whether these colonies are safe for human therapy.

Authors: Olivia Frary, Carl Hjelmen. Mentors: Carl Hjelmen. Insitution: Utah Valley University. Previous work has shown that there are significant differences in lifespan between sexes of the same species. Whether it is an XY or ZW system, the heterogametic sex dies earlier. There are thought to be many factors that contribute to the difference in lifespan between the sexes. This research aims to investigate this relationship further by digging deeper into the degree of differences between the heteromorphic sex chromosomes. We want to determine whether the degree of differentiation correlates with the lifespan differences in species. The comparison of total genome size between the sexes of multiple species are used to find the difference in the size of their sex chromosomes, which is then compared to longevity data on the species to determine a relationship. This genome size data comes from publicly available databases and literature. These comparisons are made using comparative phylogenetic methods in order to account for evolutionary relatedness utilizing a newly constructed phylogenetic tree. All analyses are completed in R and include PGLS (Phylogenetic Generalized Least Squares) and measures of phylogenetic signal (Pagel’s lambda, Blomberg’s K). We will discuss the results of the comparative phylogenetic analyses and any patterns seen regarding longevity.

Authors: Barbara Jetton, Carl E Hjelmen. Mentors: Carl E Hjelmen. Insitution: Utah Valley University. This project’s emphasis is the creation of an accessible and reusable tool to be used in broader scientific inquires of evolutionary relatedness. As of January 2023, GenBank contains 2.9 billion nucleotide sequences representing 504,000 distinct species. Despite this abundance of data, comprehensive and up to date phylogenies are lacking, impeding investigation into genetic histories and trait evolution. To address this problem, I am developing an open-source pipeline to expedite the construction of these evolutionary trees. I have a specific aim of creating a phylogeny for the order Diptera (flies) in order to investigate the evolution of the chromosome numbers for over 2500 species with chromosome count data on karyotype.org. I use R code, and the packages “reutils”, “ape”, and “seqinr”, to create reusable universal scripts which pull accession numbers from NCBI GenBank for each species based on the requested gene names. A second script was built to use the curated accession numbers to pull FASTA sequence data for each gene and write a multi-FASTA file for each gene, resulting in a comprehensive dataset necessary for alignment and phylogenetic tree construction. This effort will result in updated insights on the evolutionary history of Diptera related to chromosome numbers and can be used in further research in comparative biology. Additionally, these scripts can be used to investigate and reconstruct phylogenetic information for any species group with sequence data available on GenBank.

Authors: Michelle Stanley. Mentors: Baktybek Abdrisaev. Insitution: Utah Valley University. Due to the Russian invasion of Ukraine in 2022, much of Europe has been striving to reduce their dependence on Russian energy. Lithuania, however, began this process 11 years ago when Russia raised the price of Russian gas transported through Gazprom. Lithuanian leadership recognized the potential danger of being dependent on Russia and with the Russian invasion of Ukraine, those concerns proved to be well-founded. Through its history, Lithuania has experienced Russian oppression and has fought for its sovereignty. The history of Lithuania’s energy relationship with Russia from its declared independence from the Soviet Union in March 1990, its complete dependence on Russia for gas, and the process of becoming the first European Union country to become independent from Russian gas in May 2022. This is a case study which shows that: independence from Russian energy removes Russia’s coercive power over that country, the current European energy crisis is the culmination of long-standing European dependence on Russian energy, and that eventually European countries will have to reconcile with Russia. The article will outline three significant periods of time in Lithuania’s energy relationship with Russia: the Lithuania-Soviet Union energy relationship immediately before the fall of the Soviet Union, the process whereby Lithuania has pursued its energy independence from Russia since 1991, and Lithuania’s efforts to achieve full energy independence from Russia following the Russian invasion of Ukraine.

Authors: Cassandra Nelson, Braden Richards, Grace Van Buren, Fernando Gonzalez, Ian Kirkpatrick, Paul Dunn. Mentors: Paul Dunn. Insitution: Utah Valley University. Many organisms experience a life history pattern called ontogenescence, a high but declining mortality risk during early life. Ontogenescence can have a large effect on a population’s growth by limiting the number of new recruits to the population. Clearly, and yet paradoxically, death before reproductive maturity should be selected against, yet ontogenescence persists across many otherwise dissimilar organisms. Some intriguing hypotheses to explain this pattern have been proposed but have not been thoroughly tested. One of these hypotheses, acquisition of robustness as a function of age, is perhaps the most intuitive. The goal of our current research is to examine the validity of this hypothesis by using brine shrimp (Artemia franciscana) as a model organism.Tracking life-history traits like ontogenescence often requires the researcher to be able to follow an individual throughout its lifespan. In aquatic invertebrates, this means culturing animals individually, which can be quite difficult. Here we report on progress we are making with these culturing methods by testing how food availability and variety affect the early life mortality and development rate of brine shrimp in small-volume culture. Our null hypotheses are that neither of these variables will affect mortality or age to maturity in the animals. Our alternative hypotheses are that increased food availability and variety should lower mortality risk and age to maturity. To test our hypotheses, we hatched brine shrimp cysts in artificial seawater and placed individual artemia into wells of 6-well cell-culture plates filled with artificial seawater. We fed the shrimp one of several food treatments to see how diet affects development and mortality. In our first round of trials, we provided our animals with two different food concentrations (high food vs. low food) of the three food types we evaluated: a unicellular alga (Nannochloropsis), a cyanobacterium (Spirulina), and brewer’s yeast (Saccharomyces cerevisiae). In our second set of trials, we provided the shrimp with less variety in their diet (only two of the three food types). As the artemia developed, we conducted regular water changes and monitored development and mortality. We tracked the fate of the artemia until all individuals had either died during development or reached adulthood. Our food availability trials resulted in no significant difference in either mortality risk during development or age at maturity based on food concentrations. Trials examining the effects of limiting the variety of food types are ongoing.

Authors: Noah James, Rebekah Hakala, Todd Spencer. Mentors: Todd Spencer. Insitution: Utah Valley University. The Relationship Among Attachment Styles, Couple Satisfaction, and Perceived StressNoah James, Rebekah Hakala, Todd SpencerIntroductionAttachment styles play a pivotal role in romantic relationships. According to existing research, individuals with fearful, dismissive, or preoccupied attachment styles tend to experience elevated stress levels (Candel & Turliuc, 2021; Waring et al., 2019). Conversely, those with secure or preoccupied attachment styles report greater relationship satisfaction (Candel & Turliuc, 2021). By exploring the relationship between attachment styles, stress, and relationship satisfaction we hope to foster healthier relationships and deepen our understanding of the interaction between attachment orientations and emotional well-being (Candel & Turliuc, 2021; Waring et al., 2019).AnalysisThe purpose of the present study is to examine the relationship among attachment styles and couple satisfaction and stress. Our sample consists of 411 married individuals. Participants completed the Perceived Stress Scale - short form (PSS-4; Cohen, Kamarck, & Mermelstein, 1983), The Relationship Questionnaire (RQ; Bartholomew & Horowitz, 1991), and Couple Satisfaction Index (CSI-4; Funk & Rogge, 2000). Results of the one way Anova were significant F (3,407) = 10.48, p=<.001. There was a significant difference in levels of relationship satisfaction among attachment styles. Secure attachment (M=15.66, SD = 2.44), fearful attachment (M=14.09, SD = 3.30), preoccupied (M=15.75, SD = 2.35), and dismissing (M=14.05, SD = 3.54). Results of the Bonferroni Post-hoc analysis indicated a significantly higher levels of couple satisfaction than fearful and dismissing attachment styles (p.<.001). There was no significant difference in level of relationship satisfaction between Secure and preoccupied attachment styles. Additionally, there was a significant difference in levels of perceived stress among attachment styles. Secure attachment (M=11.79, SD = 2.47), fearful attachment (M=13.91, SD = 2.55), preoccupied (M=13.20, SD = 3.35), and dismissing (M=11.81, SD = 3.54). Results of the Bonferroni Post-hoc analysis indicated a significantly lower levels of stress among securely attached compared to fearful and pre-occupied attachment styles (p.<.001). There was no significant difference in level of stress between Secure and dismissing attachment styles. Results provide empirical support that secure attachment styles tend to be beficial for both relationship satisfaction and perceptions of stress. Further, dismissive attachment styles reported similar levels of stress as securely attached participants but had the lowest level of relationship satisfaction.

Authors: Alley Fausett, Megan Ritter Martin, Kristin Davenport, Malisa Brooks, Kent Hinkson. Mentors: Kent Hinkson, Malisa Brooks. Insitution: Utah Valley University. Background: University students oftentimes face challenges with well-being and mental health, including depression, anxiety, sleep problems, PTSD, and suicide ideation/behaviors. However, previous research in some areas such as depression and its impact on student’s academic performance has remained generally inconclusive because of the contradictory findings that have often been reported. For example, one study will find a moderate association between grade point average (GPA) and depression, while another will in turn fail to find a significant relationship. Thus, more research is needed beyond simple correlations between GPA and depression to understand possible mechanisms and pathways. The current research hypothesizes that depression symptoms will either not be related or be weakly related to GPA but will be significantly associated with several possible mediating variables such as “turning in assignments late”, “having withdrawn from a class”, and “being placed on academic probation.”Methods: University students (n = 1,359) were recruited to participate in this study from dozens of universities across the United States in 2021. Data was collected anonymously and confidentially through a targeted online survey. Students were compensated with a $10 Amazon gift card for their participation. Zero order correlations were conducted to examine the relationship between variables of interest, which may lead to possible mediating pathways.Results: No statistically significant correlation was observed between depression symptoms and academic performance among students. Yet, several other measures of academic performance beyond GPA were found to have negative relationships with depression including five questions of particular academic activity and items such as withdrawing from class, being placed on academic probation, etc. Further, the strength and significance of the relationship between depression symptoms and these measures of academic performance were stronger and more pronounced in those in their Freshman and Sophomore years. Additionally, the relationships were weaker during their Junior and Senior years, and practically non-existent in graduate programs.Conclusion: In summary, our study contributes to the growing number of often-contradictory studies demonstrating a sometimes-yes, sometimes-no relationship between depression and GPA. However, our study goes beyond this binary comparison to explore other variables of academic performance and found several significant relationships that can help the field better understand the impacts of depression on student’s performance. These findings emphasize the importance of addressing mental health for better academic outcomes for university students. Implementing targeted support systems and interventions within educational institutions could benefit students’ overall well-being and improve academic performance.

Authors: Audrey French, Carl E Hjelmen. Mentors: Carl E Hjelmen. Insitution: Utah Valley University. Chromosome numbers are often one of the first pieces of genetic information we collect about the genome of an organism. These chromosome numbers vary widely across the tree of life and will change due to fission and fusion events, where the number increases or decreases, respectively (Blackmon and Demuth 2015, Perkins et al. 2019, Sylvester et al. 2020, Morelli et al. 2022). As the Drosophila species group has been a model for more than 100 years, there is a plethora of information on their karyotypes, including number, chromosome shapes, and sex chromosome systems. The shapes of chromosomes vary and can be described as metacentric (v), submetacentric (j), telocentric (rod), and small and heterochromatic (dot). In the Drosophila genus, sex chromosome systems can be neo-sex, XY, and X0. The numerous species of Drosophila can be compiled into the major subgenera of Drosophila and Sophophora, which have diverged for around 40-65 million years. The karyotype data of the Drosophila genus has been compiled into an open-source database. I am using this database to find the rates of chromosome number change in the Drosophila genus. This analysis is done through the chromePlus package in R (Blackmon et al., 2019) which will perform a comparative phylogenetic analysis, and a phylogeny of 152 species in the Drosophila genus (Hjelmen et al., 2019). Using these available tools, I can compare rates of fusion and fission between sex chromosome systems and subgenera. This could expand the knowledge on evolution of chromosomes and genomes.

Authors: Nathan Klundt, Violeta Vasilevska. Mentors: Violeta Vasilevska. Insitution: Utah Valley University. In electrical power networks, phase measurement units (PMUs) are sensors used to monitor the network. However, these PMUs are very costly, hence the electric company are interested in using the minimum number of PMUs that will ensure that they can observe the whole network. This real-life problem is modeled in graph theory as a graph coloring game. Namely, the power domination problem [2, 3] in graph theory is concerned with finding a minimum number of these sensors needed to color (observe) the entire graph (network) according to a set of rules. We consider two variants of this coloring problem. The k-fault-tolerant power domination [3] is asking to find minimum number of PMUs needed to observe (color) the whole network (graph) even when k number of the PMUs are faulty but allows only one PMU to be placed on an electric node (vertex). The other variant, called robust power domination [1], asks the same as fault tolerant power domination, but allows for multiple PMUs to be placed on the same electric node (vertex). In this presentation, we introduce these coloring problems through examples, and provide some theoretical bounds on the minimum number of PMUs needed for various families of graphs for both k-fault-tolerant and robust power domination problems. References: [1] Beth Bjorkman and Esther Conrad. (2023). Introduction to Robust Power Domination. arXiv:2305.13430. [2] Dennise J. Brueni and Lenwood S. Heath. The PMU placement problem. SIAM Journal on Discrete Mathematics, 19(3): 744-761, 2005. [2] Teresa W. Haynes, Sandra M. Hedetniemi, Stephen T. Hedetniemi, and Michael A. Henning. Domination in graphs applied to electric power networks. SIAM Journal on Discrete Mathematics, 15(4): 519-529, 2002. [3] Kung-Jui Pai, Jou-Ming Chang, and Yue-Li Wang. Restricted power domination and fault-tolerant power domination on grids, Discrete Applied Mathematics, 158(10):1079–1089, 2010.

Authors: Mikaela Cowles, Joseph Jensen. Mentors: Joseph Jensen. Insitution: Utah Valley University. We are developing a newly calibrated distance ladder based on surface brightness fluctuations (SBF) to decrease systematic uncertainties in the current measurement of the Hubble Constant. James Webb Space Telescope (JWST) observations of 14 nearby elliptical galaxies are now being observed to measure a new zero point for SBF distances independent of Cepheids. We have recently acquired 69 images of massive early-type galaxies using the Hubble Space Telescope (HST). These HST distances have doubled the number of galaxy distances measured using SBF and will be combined with past SBF surveys to calculate the Hubble Constant to help resolve the Hubble tension. These distances will be added to the Cosmicflows-4 database to help map the mass distribution in the local universe. This project will continue with the allocation of an additional 123 HST orbits, from which we expect to add ~40 galaxies in 2024.

Authors: Asher Mitchell, Neil Chilson. Mentors: Neil Chilson. Insitution: Utah State University. With the rapid adoption of Artificial Intelligence marked by the release of OpenAI’s GPT model and a number of similar models, concerns are being raised about the potential for centralization of control over the technology, which could have implications like control of user data, increased political influence, lack of user choice, and several other potential issues.This paper focuses on an alternative to the currently proposed solutions to this threat; creating a separate regulatory agency for the technology, or legislating conformity to specific standards. We focus instead on different interoperability-based solutions that allow end users to tap into the source code, giving them the ability to ensure control of their data and allowing for competition in the AI market.

Authors: Kacey Rowe, Rachel Arocho, Allison Ensign. Mentors: Rachel Arocho, Allison Ensign. Insitution: Utah Valley University. Mental health amongst IBD patients is often overlooked and forgotten when they are dealing with their personal physical symptoms. There is little research on the benefits of social support for IBD patients. In online forums, it appears some IBD patients report feeling depressed and having negative thoughts about living life on a day-to-day basis. Indeed, prior research has found associations between IBD and mental health; for example, one study found that 17.3% of IBD patients had lifetime suicidal ideation, which is much higher than the general population at a 4.0% rate (Xiong et al., 2022). Another study did physical and mental exams and found on average, people with higher depression symptoms were six times more likely to have their disease be more active (Marrie et al., 2021, para. 16). Due to the high rate of depression, it would be beneficial to design a website to track symptoms and create online forums giving people the opportunity to connect with peers who have IBD. IBD can drastically affect a person’s life, even in a mild case. According to the CDC, IBD is estimated to affect 3 million US adults, a little over 1% of all Americans (Xu et al, 2018). Few people in the 1% category who struggle with IBD have connected online with others who are experiencing the same autoimmune disease. Although prior research has identified the association between physical IBD symptoms and mental health, little has been proposed to help these patients. Our goal is to create a form of social support for people who have been diagnosed with IBD. For 30 days, we will take 20 participants and have them record their symptoms and thoughts through quantitative and qualitative data. To do this, the participants will be asked to use a website which we have created from scratch. They will be using the Patient Health Questionnaire-9 (PHQ-9) and the Short Inflammatory Bowel Disease Questionnaire (SIBDQ). The PHQ-9 will be used to check in on mental health, while the SIBDQ will focus on the physical symptoms of IBD. Each week, they will participate in forums on the website, which will allow them to connect online and anonymously with others who share their experiences. At the start of the month, daily throughout the study, and at the end of the month, participants will report on their health symptoms through a combination of the measures named above and short tracker questions. (Note: Project is still under IRB approval, we aim to start participants by the end of the year.)

Authors: Aubrilin Johnson. Mentors: Yu Ya Liang. Insitution: Utah Valley University. Drought stress negatively affects the growth, survival, and production of crops. Developing drought-tolerant cultivars is an important strategy for maintaining crop yields under undesirable water conditions. Tomatoes are an important crop both grown worldwide and here in Utah. Improving drought tolerance in tomato plants can increase yearly yields and reduce the amount of crops lost during the periodic droughts that Utah has. A way to develop drought-tolerant tomatoes may be through identifying and utilizing genes that enhance survival and growth rates under stressful water conditions. In this study, we will use two varieties of tomato, including drought-tolerant wild species, and susceptible commercial varieties. According to previous studies, drought during late the vegetative stage will significantly reduce the yield, therefore this study will focus on that. Using RNA-seq to find the difference in gene expression between the control and drought groups. Identifying differences will facilitate in finding candidate genes that may have a potential role in drought resistance. Then take the top cultivar and find the specific drought-resistant gene, which can then be used by Utah tomato growers to yield more resistant crops.

Authors: Erica Webb, Joseph Price. Mentors: Joseph Price. Insitution: Brigham Young University. Blocking is a strategy used in machine learning to reduce the number of comparisons that need to be considered. In this paper, we develop a blocking strategy based on the characteristics of two people in the same household. We apply this pair blocking approach to link US census records and show that it dramatically reduces the number of possible matches to consider and can directly identify millions of unique matches at the blocking step. We apply our method to linking records for a few groups that have been harder to link using previous methods, including inter-racial couples and German-born Americans. Both of these are groups that have changed how they report aspects of their identity (race of birthplace) over time. Our approach allows us to dramatically increase the match rates for these couples across adjacent census years.

Authors: Grace McFarlane. Mentors: Michael Rotter. Insitution: Utah Valley University. Thimbleberry (Rubus parviflorus) is a native shrub to North America, and can be an important component in restoring native plant communities. Members of this genus tend to have physically hard seeds and low germination rates, resulting in a lack of ability to grow this plant by seeds and increasing genetic diversity in restoration sites. We investigated six different methods in order to produce higher germination rates, tracking them over time. Using seeds from three different populations we planted these thimbleberry seeds in groups of 50 for each germination method, monitoring the rates of growth and the success rate percent. These methods included standard planting as our control, cold stratification using a fridge, scarification with sandpaper, hot water bath by boiling the seeds, acid bath with sulfuric acid, and a room temperature soak in water overnight. Our goal is to identify a standard procedure for high germination rates and success.

Authors: Eliya Rodrigues. Mentors: Grant Corser. Insitution: Southern Utah University. Does an Insecure Attachment (Anxious and/or Avoidant) to God and the Contextual Perception of God (Belief that God’s Character is Consistent with what the Bible says about God’s Character) Affect Levels of Distress? People who identify as spiritual/religious that have an attachment to God are associated with having lower levels of depression, while people that have an insecure attachment to God, and a judgmental perception of God have higher rates of depression (Bonhag, R., & Upenieks, L. (2021). The purpose of this research is to investigate if there is a relationship between the style of attachment to God, the contextual perception of God, and levels of distress. The perception element of this research question will be analyzed through the contextual perception of God as defined in appendix D. Data will be collected using Qualtrics and will have three different scales (Attachment to God Inventory, Kessler Psychological Distress Scale, and Belief of the Character of God Through a Contextual Perspective) consisting of qualitative questions and a few open-ended questions regarding the subject. Data will be analyzed through correlational statistics on SPSS. It is hypothesized that a low score of an insecure attachment to God and regarding scripture in the bible as truth for God's character will decrease levels of distress. This research needs to be conducted because it could be a way for people to evaluate their own attachment to God; the way people view God could lead them to understanding that if part of the distress they experience in their lives is from their own perception and/or attachment to God.

Authors: Lisa Bell, Kelsey Hixon Bowles, Joni Hayward Marcum, Maren Neild, Asmaa Alsharif. Mentors: Lisa Bell. Insitution: Utah Valley University. With the development of artificial intelligence and its breakthrough into the academic sphere, some might argue that artificial intelligence is a better and more effective tutor than human tutors. However, it’s important to acknowledge that peer tutoring itself aligns with High-Impact Practice (HIPs) as coined by George Kuh, and it effectively leverages each core element of HIPS. As a high-impact practice, tutoring enhances student engagement, critical thinking skills, and overall academic and personal development and success. Although evidently peer tutoring aligns with HIPs criteria, it is severely underrepresented in HIPs research and discussions, warranting a closer examination and recognition in the academic community. By integrating writing center research with High-Impact Practices (HIPs) and original Institutional Review Board (IRB) research, this presentation establishes peer tutoring as a High-Impact Practice (HIP) and underscores its significance in education, even in the era of advancing artificial intelligence.

Authors: Viridian Hellebore, Isabella Rodriguez, Stockton Palmer, Sim Perry, Justine Padilla. Mentors: Kirsten L Graham. Insitution: Southern Utah University. The examination of attitudes towards the LGBTQ+ community presents a newly found psychological concept that plays an important role in fostering social inclusion, reducing discrimination, and promoting a more accepting society. The purpose of our study is to create a measure that will assess a person's general attitudes towards the LGBTQ+ community. In the current social and political climate of the US, there are a multitude of differing thoughts, opinions, and feelings regarding the community- some that are positive, and some that are negative. Current and past research generally only focus on sexuality, requiring the need for a scale that includes all members of the LGBTQ+ community. Such past works include Attitudes Toward Lesbians and Gays Scale (Herek 1988) as well as the Index of Attitudes Toward Homosexuals (Hudson & Ricketts 1980). The Attitudes regarding Gender and Sexuality Scale (AGSS) was created to provide a single scale that will measure a variety of attitudes towards the LGBTQ+ community. The AGSS covers fear, understanding, and tolerance- related aspects of these attitudes. Before releasing the scale to the general public, the scale was given to five subjects to assess for face validity. Participants will be recruited from MTurk and social media, and will be asked to respond to 21 questions that envelop the above aspects of attitudes. The participants will answer the “Gender/Sex Diversity Scale'' (Sudson, 2022) alongside AGSS to test for construct validity. The participants will also be asked to answer two scales that are measuring different constructs to test for discriminant validity. Once all of the data has been collected a confirmatory factor analysis will be ran. To test for reliability, we will be using the internal consistency method. For this we will be testing for Cronbach’s coefficient alpha since our scale using a 7-point Likert scale. We hypothesize that AGSS will be able to measure both negative and positive attitudes that are directed towards the LGBTQ+ community. With these results further research could be conducted to evaluate the effectiveness of anti-discrimination laws, the reasoning behind these attitudes, and how the attitudes affect the wellbeing of the LGBTQ+ community.

Authors: Brandon Barlow, Ethan Reese, Victoria Vazquez, Abbi Stark, Monica Sanchez, Alex Wilson, Rain Evans, Josef Becker, Amy Chevrier, James Taylor. Mentors: James Taylor. Insitution: Utah Valley University. This study focuses on memory and learning observed in Madasgascar Hissing Cockroaches (MHC). Research on insects can be used to inform our understanding of behavior and cognitive processes and add to our existing knowledge about the studied species. While there is existing literature focused on memory and learning in many insects, cockroaches are often chosen as a model due to their adaptability, resilience, and availability. Little research on MHC currently exists, especially in regard to memory and learning or drug seeking behaviors. This study uses a mixed model design, looking at arm choice percentages and latency to choose, between the control and experimental groups and comparing trials for each subject. In earlier trials, MHC demonstrated behavior that indicated they had learned not to fear light, a naturally aversive stimulus for cockroaches. Building on data collected in earlier trials, we developed a new protocol to evaluate the MHC associative learning behaviors and to test drug-seeking as a motivated behavior. Subjects are individually tested in a T-shaped apparatus in which they have to choose an arm to go into or are timed out. Each arm has a specific reward, either a sucrose and ethanol solution (experimental group) or plain sucrose solution (control group) in the left arm, and plain sucrose solution (experimental group) or water (control group) in the right arm. Each subject is run 2 days (6 trials per day) in these conditions, and then 3 days with the left arm lengthened to test if the subjects will seek their preferred reward even if it requires traveling a greater distance.

Authors: Benjamin Holt. Mentors: York Young. Insitution: Utah Valley University. Our research group aims to create an optical system which generates tunable longwave infrared (LWIR) light as a tool for early detection of disease-indicating molecules in human tissue. This system begins with our newly constructed Nd:YVO4-type laser which generates between five and seven watts of continuous wave optical power in the near IR spectrum. This laser light will be used to drive the subsystem responsible for generating LWIR light: an optical parametric oscillator. In order for wavelength conversion to occur via a non-linear process, the near IR laser power serving as an optical drive must have an intensity on the order of tens of MW/cm2. To achieve such intensities the laser power must be concentrated into high intensity nanosecond length pulses by Q-switching the laser. Our method of Q-switching the Nd:YVO4 laser will be via an acousto-optic modulator in the laser cavity. My work to design the modulator’s kinematic mount, cooling system, and RF driver (as well as characterizing the performance of the modulator for use as a laser Q-switch) is described in this presentation.

Authors: Samantha Curnow, Carl Hjelmen. Mentors: Carl Hjelmen. Insitution: Utah Valley University. The genome sizes of insects vary immensely; some insects have only 100 million base pairs, while others reach nearly 18 billion base pairs. Many selective pressures can have an influence on genomes, but we still lack a clear understanding of which selective pressures cause such variation in genome size even within well-studied groups. To determine the influence of environmental factors on this variation, we investigate the relationship between genome size and the average natural environment of 152 Drosophila species. The nature of the relationship between genome size and environmental factors is investigated using phylogenetic models and linear analyses such as phylogenetic generalized least squares analysis. We collected data from peer-reviewed papers and publicly available datasets, such as karyotype.org and WorldClim, to compare the genome size of each species to the temperature, altitude, humidity, etc. of their natural environments using linear phylogenetic models. The distribution of these species was also visualized using geographic and biological analysis packages in R, such as geodata, to extract environmental data of specified regions, and phytools in combination with caper to run phylogenetic comparative analyses.

Authors: Roshan Abraham, Parker Jorgenson. Mentors: Daren Nelson. Insitution: Utah Valley University. Hobble Creek is an essential and valuable stream that the City of Springville and other local entities utilize for their water supply. However, the stream is also vital to the local environment and ecosystem since it one of the main streams that the June Sucker (an endangered endemic fish) spawn in each year. Numerous environmental projects and studies have been done in the lower section of Hobble Creek due to this importance. There are many springs in the region that provide water to the stream but there is a lack of consistent monitoring of the flow throughout the stream to determine how the stream is interacting with groundwater sources. Groundwater models completed by the United States Geological Survey in 2005 for the Utah Lake Watershed suggest locations where groundwater interactions should be either discharging or recharging the local aquifer. However, more recent data has suggested that many of these water interaction maps created by the USGS model are inaccurate. Using a water budget analysis, streamflow in the creek is being measured at 10 sites throughout the Hobble Creek Watershed to determine how the flow in the creek changes as it flows towards Utah Lake. Data gathered from this and previous studies show the zones of discharge and recharge shift seasonally and are not as consistent as the USGS model suggests. Recent data does confirm that Hobble Creek is gaining flow upstream and losing flow downstream, but there are portions of the river that also may be perched (do not interact with the groundwater). Further measurements and continued site investigation will be necessary to help us better manage this vital water resource.

Authors: Feyan Hoffman, Roshan Abraham, Christine Fowles, Abi Conger, Scott Jenson. Mentors: Daren Nelson. Insitution: Utah Valley University. One of the least understood part of our local water budget is the interaction of groundwater into our lakes and streams. Groundwater models completed by the United States Geological Survey in 2005 for the Utah Lake Watershed suggest zones where groundwater interactions should be either discharging or recharging into our local streams, wetlands, and other water bodies. However, more recent data has suggested that many of these water interaction maps created by the model are inaccurate, and we need a better understanding of how groundwater interacts with these water bodies. Using a water budget analysis, groundwater and surface water interactions are measured by collecting discharge measurements using flow meters at different cross sections along the Provo River and its tributaries. A gaining or losing stream may swap depending on seasonal changes, such as a particular water year being dry or wet–a stream that is typically gaining may be recharging the aquifer during a dry year. The USGS model is a good basic representation of interactions between groundwater and surface water. Our data suggests that due to highly variable water levels of Utah Lake and local groundwater levels, some discharge and recharge zones in the Provo River subbasin are likely to change over time. In addition, some sections of the river may be perched and not interact with the groundwater system at all. In order for us to better manage these important resources, further analysis of flow behaviors in the Provo River and its tributaries need to be completed. This study is part of a larger study that includes adding two new permanent flow gauging stations to provide a long term record that will enable water managers to better decipher why the groundwater and surface interactions are not fitting the USGS model.

Authors: Jacob Sampson, Joshua Goates, Brian Patchett, Abolfazl Amin, Bonnie Andersen. Mentors: Bonnie Andersen. Insitution: Utah Valley University. Noise from an interstate can reach extreme levels. The OSHA limit for when sound becomes dangerous for the health of individuals is 85 dB. At UVU there is a pedestrian bridge that goes over I-15 and the students and other pedestrians that use this bridge are exposed to sound levels that could be potentially dangerous for their hearing, along with other adverse health effects due to sound on a regular basis. Sound levels have been measured on the bridge using two different measuring devices. The first being an Extech noise dosimeter, that was worn on the shoulder of the measurer as they walked across the bridge. The second instrument used was a Larson David sound level meter. That was placed on several places along the length of the bridge at set to record for several minutes to get the sound levels at those places. Sound levels that have been recorded have ranged form 79 dB to 102 dB. The OSHA limit for occupational exposure at 100 dB is two hours while for non-occupational exposure it is only 15 minutes. This research seeks to better understand and quantify the noise exposure pedestrians experience on the bridge. Also this research hopes to find out what facts influence the sound levels on the bridge and we hope to compare our experimental results to an ANSYS model of the bridge to confirm our findings.

Authors: Spencer Brown, Shawn Pepper, Carson Draughon, Ulises Thornock, Dustin Shipp. Mentors: Dustin Shipp. Insitution: Utah Valley University. In the shadow of the 2021-2023 global energy crisis, the need for alternative sources of energy has reached an all time high. To combat this, we have developed a prototype method of harvesting kinetic energy from a door swing, converting that energy into electricity, and storing it inside a battery for future use. Our experiments have demonstrated the capability to use the energy from one door to charge a cellular phone, but we believe this method could be applied on a much larger scale. In this poster we will discuss the method, design, and measurements from our device, as well as possible future applications.

Authors: Miranda Rose, Daniel James. Mentors: Daniel James. Insitution: Utah Valley University. In this study, we investigated mechanical vibrations as a mechanism for creating uniform droplets in a free liquid jet. A stream of liquid naturally breaks into irregular droplets as it falls due to the Plateau-Rayleigh instability. In this work, droplet break-up was triggered by utilizing a motor with Arduino-based frequency control and stroboscopic visualization. The project achieved uniform droplet sizes for multiple liquids of different viscosities. Notably, this method demonstrated the creation of Dippin Dots-like confections at a STEM fair, engaging learners in the principles of physics and engineering. Future work will focus on the original goal of utilizing acoustics for droplet formation, aiming to refine the process and expand its industrial applicability.

Authors: Tyler O'Loughlin, Tyler Daynes, Erik Prazak. Mentors: Dustin Shipp. Insitution: Utah Valley University. In the realm of photon interactions with surfaces, the reflection and refraction of light depend significantly on the micro and macrostructures of the surfaces. Despite the use of various methods to attenuate light, their effectiveness remains a subject of interest. In this study, we delve into the properties that render super-black coatings, such as Vantablack, exceptionally dark compared to coatings with distinct surface characteristics but significantly altered amplitudes of reflected intensity. Our research focuses on understanding the optical properties of Vantablack; in particular, we investigate the role of the carbon nanotube microstructure in achieving its unique light-absorbing properties. Through a comprehensive analysis of our samples, we have uncovered that the carbon nanotube microstructure of Vantablack contributes to its remarkably low reflective intensity and exceptional absorption across all wavelengths of light. Notably, Vantablack displays such a high optical density that even at Brewster's angle, a reflection is virtually undetectable. This research sheds light on the intriguing properties of Vantablack and has implications for a wide range of applications where light absorption and reflection play a critical role.

Authors: Kaycie A Melville, Ashley N Egan, Yu Ya Liang. Mentors: Ashley N. Egan. Insitution: Utah Valley University. Phaseolus vulgaris (common bean) and P. lunatus (lima bean) provide protein and nutrition for millions of people across the globe. Native to the New World, these cultivated species present unique histories of multiple and independent domestications in Mesoamerica and the Andes. Brazil is the leading producer globally of these beans. With both wild and cultivated landraces present, Brazil may act as a secondary center of domestication for these two economically significant leguminous crops. Brazil's indigenous populations’ cultivation efforts may include both wild and cultivated germplasm, potentially contributing to the continued domestication of these crops to create novel shapes, sizes, and colors that are grown and hand-selected by the natives in these regions. To investigate Brazil as a secondary domestication center of beans, this research addresses several key questions: Are signals of past and current domestication processes discernible in current Brazilian cultivars of these species? Can we unravel the relationships between these Brazilian crops and their primary centers of domestication? What genetic differences are underpinning domestication? How diverse are Brazilian cultivars? How do they relate to wild and related material and species? This work focuses on 48 accessions of P. lunatus and P. vulgaris from Brazil, including wild material and two accessions from two related species, P. polystachios and P. filiformis from North America. Whole genomes were resequenced using Illumina sequencing and compared against the published genome of Phaseolus vulgaris to identify SNPs in each accession. Whole chloroplast genomes were also assembled and compared. Phylogenetic analyses, network analyses, population structure, and tests for neutral and positively selected mutations were conducted, revealing distinct clades for both P. vulgaris and P. lunatus with clear links to their Andean and Mesoamerican origins. Genetic diversity was assessed both within (cultivated vs wild) and between P. vulgaris and P. lunatus, demonstrating significant variations, not only between species but also within each species, suggesting the influence of diverse selection pressures and local adaptation during the domestication process. McDonald-Kreitman tests analyze and locate possible domestication genes from the library of SNP’s between outgroups (Phaseolus filiformis and Phaseolus polystachios) and between species. Overall, this research advances our understanding of the intricate process of domestication in P. vulgaris and P. lunatus, highlighting potential ongoing domestication in Brazilian cultivars, unraveling their genetic relationships, and shedding light on the complex interplay of genes that underpin domestication and diversity. These findings contribute to the broader understanding of crop evolution and have practical implications for crop improvement and conservation.

Authors: Joshua Stubbs, Annette Lewis, Kendall Holcomb, Barbara Suassuna Schincariol. Mentors: Geoffery Zhan. Insitution: Utah Valley University. Does Greater Phylogenetic Distance Affect Competition Outcomes in Fungal Communities?Annette Lewis, Kendall Holcomb, Bárbara Suassuna Schincariol, Josh Stubbs, Geoffery Zahn PhDFungi play a critical role in decomposition, affecting nutrient cycling at a global scale. Saprotrophic fungi competitively decompose dead organic matter. However, the role of phylogenetic relatedness on interspecific competition in fungal communities has not received much attention. The theory of phylogenetic over-dispersion suggests that species within a community tend to be less related than expected by chance, therefore limiting competition due to functional redundancy. Similarly, Darwin’s naturalization hypothesis suggests that taxonomically distinct invaders might experience reduced competition and resistance. In this study, we chose three different saprotrophic fungal species with varying relatedness: Aspergillus niger and Fusarium keratoplasticum (from the same family), and Pleurotus ostreatus (from a different phylum). These species were chosen based on decomposition abilities and phylogenetic distances. These species were cultured and placed in seven combinations to assess their ability to decompose and compete as individual fungal populations and as combined communities (e.g., A, B, A+B, B+C, and A+B+C). Each species was placed near a sterilized piece of paper such that competition was evaluated by analyzing the paper coverage in Petri dishes over three weeks. Each Petri dish was analyzed individually based on the average percentage of paper covered and, within combined communities, the percentage of paper each species covered. Interactions between each species and the percentage of the paper covered was recorded for further analysis. Assessing paper coverage allows for the observation of any potential competitive inhibition of decomposition. We hypothesize that decomposing and competitive abilities would be the strongest with A. niger. Despite the fast growth rate of A. niger individually, preliminary results suggest that it was outcompeted when paired with other species. This research highlights the potential nuances in fungal community interactions influenced by phylogenetic relationships, shedding light on the principles of phylogenetic overdispersion and Darwin’s naturalization hypothesis.

Authors: Anna Wilkinson, Feyan Hoffman, Roshan Abraham. Mentors: Daren Nelson. Insitution: Utah Valley University. Remote sensing technologies, involving drone and satellite imagery, have recently begun to be used in hydrologic research with various applications. The technology is relatively easy to obtain, is noninvasive, and helps minimize cost, access, and safety concerns related to the study of wetlands. The thermal and multispectral imagery can provide unique datasets that expand understanding into groundwater and surface water interactions. As part of this project, we are exploring remote sensing applications within the hydrology of wetlands surrounding Utah Lake, a large freshwater lake within Utah Valley. The project has involved the collection of thermal and multispectral drone imagery from 2021 to 2023. Thermal imagery collected in these areas has assisted in the determining of hot and cold spring locations and how these springs have shifted due to changes in groundwater levels and lake levels. In addition, this imagery has shown how physical anthropogenic influences have also impacted the location and stream channels associated with these springs. Multispectral imagery is used to understand the health of phreatophytes near the lake during dry and wet years to be used as an indicator for groundwater level changes. Even though much of the data is still preliminary and part of a long-term study these remote sensing technologies are already showing how these groundwater systems adapt to seasonal and anthropogenic changes.

Authors: Cole Statham, Katelyn Thomas. Mentors: Michael C Rotter. Insitution: Utah Valley University. Datura (Solanaceae) is a potent hallucinogenic genus of plants that has varied uses across cultures. Tropane alkaloids in these plants are potent compounds that, depending on dosage, can offer the user a psychoactive experience or cause severe reactions including death. For some, Datura is little more than an ornamental flower or an ideal source to have a psychedelic experience. While for others it is a sacred plant, used on special occasions, and only permitted to be handled by those who have been prepared and trained to do so. An explanation for these cultural differences could be that cultures with taboos surrounding this plant are likely those that have species of Datura with variable levels of tropane alkaloids, while cultures without taboos are likely to have species with consistent levels of tropane alkaloids. Here we focus on six species of Datura using literature reports of tropane alkaloid contents and compare the variability in these phytochemicals to cultural uses of these plants. We predict that societies with cultural taboos will be more likely to be those with species that have higher variability in tropane alkaloids.

Authors: Vy Dao, Devin Taylor, Danielle Taylor. Mentors: Devin Taylor, Danielle Taylor. Insitution: Utah Valley University. Epilepsy is one of the most common neurologic disorders affecting about 60 million people worldwide (Chen et al., Poduri and Lowenstein, 2011). Currently in the field, there are various methods being used for mechanical seizure induction using Drosophila melanogaster. This variability makes it difficult to compare findings from different research studies. We employed various mechanical seizure induction techniques currently used in Drosophila epilepsy research. We focused on both group testing as well as individual testing using different anesthesia parameters. For individual testing, epileptiform activity and locomotor activity were recorded in adult male Drosophila through mechanical seizure induction using a vortex assay at either 24-hours post- or 1-hour post-CO2 anesthesia. We also evaluated the time for 50% of adult drosophila recovery as indicated by a group climbing assay (10 flies per group) following mechanical seizure induction using a vortex assay at either 24-hours post- or 1-hour post-CO2 anesthesia. Finally, for each of the above testing parameters, we not only tested wild-type (WT) flies, but also multiple positive control seizure phenotype flies commonly used in mechanical seizure induction studies using Drosophila melanogaster. We found significant effects in both CO2 induction methods as well as in different genetically induced seizure phenotypes.