Fine Arts

Authors: Stone Smith, Ben Belt. Mentors: Sally Rocks. Insitution: Utah Valley University. Microplastics are particles less than 5 mm in length. These particles act as a vector for heavy metal and carcinogens, which have negative effects on human health. Often invisible to the naked eye, microplastics are extremely mobile and have been found in the most extreme parts of the planet, from the peak of Mount Everest to depths of the Mariana trench. Because microplastics are readily transported via air and water, microplastics have been found in every location exposed to wind or rain. However, one place that is sheltered from wind and rain are caves. In some subterranean environments, the earth acts as a natural filter of the water that descends into caves. It is suspected that caves will have lower levels of microplastics present in their environments compared to surface environments. However, another mechanism for microplastic introduction is human activity. Humans utilize plastics in relatively all synthetic items. Everything from clothing to packaging to regular household items employs the use of plastics, which can abrade to release microplastics. The goal of this research is to determine if caves with high levels of human visitation have higher concentrations of microplastics than caves with little to no human visitation. Analysis of subterranean water samples will reveal if microplastics are invading cave environments regardless of human activity, and through what mechanisms. A consequence of microplastic pollution in cave environments is the capture of plastic particles within mineral matrices. As mineral rich water evaporates in caves, the minerals left behind form speleothems that could capture plastic particles and thus preserve them (e.g. stalactites, stalagmites, soda straws, etc.). With a better understanding of the presence or absence of microplastics in caves, efforts can be made to minimize plastic accumulation and prevent microplastics from entering the geologic record of the cave.

Authors: Iris Cepeda, Tabitha Weir, Jonathan Cook, Honey Ordonez. Mentors: Olga Kopp. Insitution: Utah Valley University. Solanum lycopersicum (tomatoes), are beloved herbaceous crops well known for their red bearing fruits. They are best grown in well-draining soils with access to full sun and are endemic to South America but grow all across the world where the conditions allow. Tomato fruits provide a quality source of phytochemicals and antioxidants such as lycopene, vitamin C, beta-carotene, and phenolic compounds which can substantially improve one’s healthy diet. S. lycopersicum are members of the nightshade family, which are known for their production of often toxic glycoalkaloids. The presence of these compounds usually deters herbivores by increasing their concentration as a defense response. There are, however, herbivores like Manduca sexta (tobacco hornworms) that feed on members of the nightshade family by dealkylating these glycoalkaloids. It is hypothesized that plants have the ability to modulate their defensive response based on queues from the environment and from their herbivore pests. Our research objective is to determine whether a S. lycopersicum plant undergoing defoliation from M. sexta will have the same molecular response as a plant undergoing simulated herbivory. Four treatment groups will be used with each treatment group having 8 replicates. Before treatment begins, the groups will be isolated by a non-permeable surface so air-borne defense signals from one group do not elicit a response in another. In the herbivory treatment group, M. sexta will be allowed to feed for a 24-hour period to account for diurnal and nocturnal herbivory, as well as high plant enzymatic response. Damage treatment 1 will simulate herbivory using a hole-punch, avoiding the midvein. Damage treatment 2 will simulate herbivory using a single transverse cut across the leaf blade and midvein. The undamaged control will have no herbaceous or mechanical damage done. After the treatment period, half of the leaf matter from all groups will be collected and frozen in liquid nitrogen to preserve all metabolic processes and GCMS will be used to analysis metabolite differences. The other half of the leaf matter will be analyzed using UV-spectrophotometer analysis with bromocresol green to compare the glycoalkaloid expression between the treatments. It’s expected that there will be a reduced expression of the defense glycoalkaloids in the herbivory group of S. lycopersium when compared to the two defoliation groups. While the three treatments could respond similarly to their treatments by primary metabolite expression, the type of defoliation could have a greater effect on this than the herbivory group.

Authors: Joshua Stubbs, Annette Lewis, Kendall Holcomb, Barbara Suassuna Schincariol. Mentors: Geoffery Zhan. Insitution: Utah Valley University. Does Greater Phylogenetic Distance Affect Competition Outcomes in Fungal Communities?Annette Lewis, Kendall Holcomb, Bárbara Suassuna Schincariol, Josh Stubbs, Geoffery Zahn PhDFungi play a critical role in decomposition, affecting nutrient cycling at a global scale. Saprotrophic fungi competitively decompose dead organic matter. However, the role of phylogenetic relatedness on interspecific competition in fungal communities has not received much attention. The theory of phylogenetic over-dispersion suggests that species within a community tend to be less related than expected by chance, therefore limiting competition due to functional redundancy. Similarly, Darwin’s naturalization hypothesis suggests that taxonomically distinct invaders might experience reduced competition and resistance. In this study, we chose three different saprotrophic fungal species with varying relatedness: Aspergillus niger and Fusarium keratoplasticum (from the same family), and Pleurotus ostreatus (from a different phylum). These species were chosen based on decomposition abilities and phylogenetic distances. These species were cultured and placed in seven combinations to assess their ability to decompose and compete as individual fungal populations and as combined communities (e.g., A, B, A+B, B+C, and A+B+C). Each species was placed near a sterilized piece of paper such that competition was evaluated by analyzing the paper coverage in Petri dishes over three weeks. Each Petri dish was analyzed individually based on the average percentage of paper covered and, within combined communities, the percentage of paper each species covered. Interactions between each species and the percentage of the paper covered was recorded for further analysis. Assessing paper coverage allows for the observation of any potential competitive inhibition of decomposition. We hypothesize that decomposing and competitive abilities would be the strongest with A. niger. Despite the fast growth rate of A. niger individually, preliminary results suggest that it was outcompeted when paired with other species. This research highlights the potential nuances in fungal community interactions influenced by phylogenetic relationships, shedding light on the principles of phylogenetic overdispersion and Darwin’s naturalization hypothesis.

Authors: Elise Bennett, Megan Frisby, Rob Hess, Max Taylor, Erin Riggs, Alma Laney. Mentors: Alma Laney. Insitution: Utah Valley University. Beet curly top virus (BCTV) is a highly destructive virus found in most of the Western United States characterized by curling of the leaves, yellowing, and lack of produce. Historically, BCTV was a key factor in the decline of sugar beet production in Utah, formerly a large factor in the state economy. Commonly found in beets, tomatoes, and peppers, BCT is vectored by the beet leafhopper, Circulifer tenellus, as it feeds on the phloem tissue. Novel hosts of this virus found in Utah include sunflower, mallow, eggplant, hemp, big sagebrush, and Solanum jamesii, the Four Corners potato. Sunflowers showed symptoms of curled leaves and stunting. Similarly, the eggplant developed shriveled leaves and a mosaic at the leaf tips. Four Corners potato, a staple food source of the Indigenous peoples of the Southwest, displayed strong symptoms including stunting, leaf yellowing, and leaf curling. Weedy species and other garden plants displaying various viral symptoms were also collected for testing. Detection was done using published BCTV detection primers and rolling circle amplification with the use of Phi29 polymerase to prepare the genome for whole genome sequencing. A 518 bp region was sequenced to 4x coverage from eight host samples. To ensure the validity of diagnosis, a triple antibody sandwich ELISA was performed confirming the results. Considering the history of BCTV in relation to the agriculture industry of Utah, the discovery of novel hosts is cause for concern since this virus is adaptable and limits crop yield. The diagnosis of BCTV in native plants, such as the Four Corners potato and the big sagebrush, is pressing as it these local flora are unique to the region. The verification of BCTV in these novel hosts allows for the agriculture industry of Utah to prepare prevention methods including the monitoring of beet leafhoppers and increased integrated pest management strategies are needed to protect our local environment.

Authors: Hayley Karns, Dylan Bryant, Noah Christensen, David Kirby, Sarah Marchant, Sarah Nicholas, Emmaline Saunders, Hannan Thomas, Nathan Smith, Dan Cavan, Daniele Ghiretti. Mentors: Alessandro Zanazzi. Insitution: Utah Valley University. Arthropods play a crucial role within terrestrial ecosystems by virtue of their substantial consumption of plant matter, their status as primary prey for various organisms, their function as key recyclers of organic material and nutrients in ecosystems, and their pivotal role as primary pollinators. Ground beetles (Coleoptera: Carabidae, or “Carabids”) represent a prominent and highly diverse taxonomic assemblage among arthropods inhabiting temperate forest floors. Despite their significance in these ecosystems, little is known about their trophic structure and level. These knowledge gaps primarily arise from the limitations associated with traditional investigative methods. For instance, the observation of feeding behavior in carabids proves challenging due to their diminutive size and predominantly nocturnal foraging habits. These uncertainties hinder the attainment of an understanding of community structure and trophic interactions both within the arthropod realm and with the vertebrates in their ecosystem. As a result, this research aims to gain a greater knowledge of the trophic structure of carabids using 15N/14N and 13C/12C isotopic analysis. Ground beetles were collected using pitfall traps placed near Big Springs Park (Provo Canyon, northern Utah) in September 2023. The traps were made with two plastic cups: a smaller cup was suspended inside a bigger cup. The smaller cup was partly filled with vinegar to attract the insects whereas the bigger cup was partly filled with monoethylene glycol, a compound that has been shown to preserve the insects without affecting their isotopic composition. Insect samples were collected over a period of four weeks. The beetles were then cleaned with a 2:1 mixture chloroform and methanol, dried, ground in an agate mortar and pestle, and placed in tin capsules for the isotopic analyses. Once the results are obtained, mixing model analysis will allow us to estimate the proportions of different food items based on the stable isotope ratios of consumers and food items and on known fractionations. This knowledge will contribute to a greater understanding of trophic structures and carbon pipelines in local ecosystems of northern Utah, and to a better prediction of ground beetle response to ongoing global warming and the associated changes in forest environments.

Authors: Kendra Johnson. Mentors: Dannelle Larsen-Rife. Insitution: Utah Tech University. This study's objective is to examine how sleep quality is related to attachment and any moderating effect from social support. Sleep quality affects multiple aspects of life including physical and mental health. About 40% of the population is affected by sleep complaints (Palagini et al., 2018). Over time, disturbed sleep can become a risk factor for mental illness and substance use disorders (Adams et al., 2014). According to Gur-Yaish et al., (2020), negative interactions related to attachment disturbances negatively affected sleep quality. Individuals with insecure attachment may be more prone to insomnia and sleep disturbances (Palagini et al., 2018). People with anxious attachment have difficulty trusting that others will be there for them when they are in need. Insecure attachment is measured on two scales: anxious and avoidant. Because of this fear, people with insecure attachment may engage in behaviors intended to reassure them of the other person’s trustworthiness. An individual with avoidant attachment tends to resist close relationships, fearing that others are not reliable (Bowlby 1982). Adults in romantic relationships tend to behave similarly to children and mothers when it comes to attachment pairing (Fraley & Shaver, 1998). When people have insecure attachment, they may be highly activated by relationship ruptures, leaving them anxious and vulnerable to poor sleep quality. The resulting poor sleep quality may be moderated by high levels of outside social support, though with high attachment avoidance, the benefits of social support are less influential. Participants (N = 94) completed the Experiences in Close Relationships-Revised (ECR-RS) scale to measure attachment, the Pittsburgh Sleep Quality Index (PSQI), and the Social Support Questionnaire-Short Form (SSQ6). Findings reveal that though the correlation between insecure attachment and sleep was small, the addition of social support revealed some interesting patterns that give insight to the relationship between attachment, sleep, and the effects of social support.

Authors: Hunter Mitchell, Grace Carsey, Maria Balaceanu, Peg Bird, Bridger Lisonbee, Dannelle Larsen-Rife. Mentors: John Jones. Insitution: Utah Tech University. Early experiences in the home have long-term effects on health and well-being. This study will examine the connections between parenting styles and coping mechanisms, shedding light on their implications for child development and adult mental health and well-being. Parenting styles looked at in this study, specifically authoritarian has varied views on expectations, discipline, and rewards (Doepke & Zilibotti, 2017). Parenting styles have been shown to impact how children express themselves, and more specifically their expression of anger (Radomir-Beliţoiu, 2019). The expression of anger may indicate a deficiency in healthy coping skills. When individuals are found without healthy ways of coping, they become increasingly vulnerable to mental health disorders and poor relationship satisfaction. Based on our current understanding we believe that an authoritarian parenting style will be positively correlated with unhealthy coping mechanisms such as avoidance, substance use, and self-criticism. Adult participants (N = 100) varying in ethnicity and age will be recruited to complete the Brief Cope Questionnaire (Carver, 1997) and an adaptive version of the Parenting Styles and Dimensions Questionnaire (PSDQ; Robinson, Mandleco, Olsen, & Hart, 2001) to assess their parent's style of parenting and their ability to cope with challenges. We will run an ANOVA on the collected data using SPSS. We expect to see a correlation between parenting styles and children’s coping skills. It is anticipated that there will be a strong positive correlation in unhealthy coping skills and parental strategies. This study may suggest the importance of educating children and young adults in healthier coping skills and adults in healthy parenting strategies. This education may aid in reducing the frequency and intensity of mental health crisis in adults.

Authors: Willow Norton, Kaleb Cox, Jaycen Sunderman, Jessalyn Warhurt. Mentors: Kirsten L. Graham. Insitution: Southern Utah University. Positive awe is a feeling of wonder, insignificance, reverence, etc., that is stimulated by man-made or natural events, objects, or circumstances. Monroy & Keltner list the five primary domains of awe as nature, spiritual engagement, music, dance, and psychedelics, but a scale determining the exact origin points of awe has not yet been developed. Upon researching awe more and determining that it is a universally felt emotion, we started looking at where awe may stem from, or what causes it. We have found that there are different types of awe, or that different populations may be able to feel it differently, but we could not find a measure of where it originates. In our study, we attempted to determine the sources from which people experience positive awe by developing the Positive Awe Origin Scale (PA-OS). While developing the PA-OS, we delivered our scale to five peers who tested the face value of our scale about readability and subject matter, and we then edited our scale based on the feedback we received. In the present study, participants will be recruited through social media and MTurk to complete an online survey, via Qualtrics. This survey will include likert scale items rated 1 to 5, with 1 being “strongly disagree” and 5 being “strongly agree.” Psychometric evaluation of results will include a confirmatory factor analysis and an internal consistency measure using Cronbach’s alpha. Discriminant validity will be measured as we correlate our scores with other, unrelated, scales included in the Qualtrics survey, which measure different constructs. Convergent validity will be measured as participants complete an awe subscale of the Dispositional Positive Emotion Scale (DPES). We anticipate that the PA-OS will help further identify the various sources of awe and lead to improvements in the research.

Authors: Peg Bird, Maria Balaceanu, Grace Carsey, Tiffanee Suhaka, Hunter Mitchell, Ives Hong, Tara Caplin, Ed Wu, John T Jones, Dannelle Larsen-Rife. Mentors: John T Jones. Insitution: Utah Tech University. This study examines the relationship between perfectionism and self-compassion. Perfectionism may be beneficial, but often promotes unrealistically high self-imposed standards, a preoccupation with making mistakes, and a compulsivity toward order, leading to self-criticism and self-loathing. Perfectionism results in persistent feelings of failure, indecision, procrastination, and shame, which negatively impact relationships, work-life satisfaction, and overall well-being (Enns et al., 2001; Frost et al., 1993). Self-compassion is directing warmth and care toward oneself during times of suffering and comprises three core components: self-kindness (offering oneself warmth during adversity), common humanity (acknowledging shared suffering), and mindfulness (approaching the experience of pain with presence and curiosity) (Neff, 2003). Self-compassion is associated with mental health and well-being, including a stable sense of self-worth, increased resilience, life satisfaction, and perspective-taking. Furthermore, self-compassion negatively correlates with anxiety and depression, reduced fear of failure, perfectionism, and self-criticism (Barnard et al., 2011; Neff, 2003; Neff et al., 2006). This correlational study will recruit approximately 100 adult participants to evaluate associations between perfectionism and self-compassion. Participants will complete the Frost Multidimensional Perfectionism Scale and the Self-Compassion Scale (Frost et al., 1990; Neff, 2003). We anticipate a negative correlation between self-compassion and perfectionism, with higher levels of self-compassion corresponding with lower levels of perfectionism. If this prediction is supported by the data, the study’s outcomes will extend existing research on the potentially deleterious effects of perfectionism and pave the way for the development of interventions aimed at improving mental health outcomes among individuals adversely impacted by this trait.

Authors: Samantha Denbow, Katie Finlinson, Carley Grube, Joseph Johnson, Tanner Dougherty. Mentors: Kirsten L Graham. Insitution: Southern Utah University. Religious affiliation is rapidly declining (Engelman, 2019). Given that religions often perpetuate an in-group out-group dynamic, bidirectional prejudices can be seen between both people who are a part of and people who are not a part of religion (Jackson & Hunsberger, 1999). One explanation for this phenomena of leaving religion and increased prejudice is that there is a shift in attitudes towards religion. Attitudes affect behavior and may explain why people are leaving religion and becoming more prejudiced towards religious and non-religious groups. Our scale Attitudes Towards Religion (ATR) aims to measure attitudes towards religion to better understand why these cultural shifts are happening. We have defined attitudes as “a mostly enduring organization of thoughts, feelings, and behavioral tendencies directed towards objects, groups, events, or symbols” (Breckler, 1984; Hogg & Vaughan, 2005). Little research has been done to determine attitudes as a three part model (affect, cognition, and behavior) when examining religion. The current measures used to examine attitudes towards religion are less defined and fail to address the tripartite model of attitudes by relying more on affective and cognitive components of attitudes (Breckler, 1984). One such scale is called the Astley–Francis Scale of Attitude Towards Theistic Faith, which measures attitudes through affective items on a Likert scale. As demonstrated through this scale and others, many measures are also limited to attitudes of theistic, western religions, rather than encompassing nontheistic religions (Astley et al., 2012; Szydłowski et al., 2021). Attitudes are important to research because they impact daily choices in every individual’s life. Our study sample will be recruited through the Amazon Mechanical Turk survey platform and researchers’ social media pages. We predict our scale will measure attitudes towards religion to further inform current literature concerning religion and religious exit. We will examine reliability in our scale by testing Cronbach's alpha to ensure internal consistency. A small pilot group of 5 undergraduate peers reviewed the items for clarity, readability, and face validity. We then made changes to the items based on the feedback. Because our measure was developed according to a well-established tripart theory of attitude, a confirmatory factor analysis will be used to assess initial factor structure and how well our model fits the data. In addition, we will examine convergent validity by comparing the Astley–Francis Scale of Attitude Towards Theistic Faith with our scale. Discriminant validity will be examined by comparing other non-related scales that measure different constructs with our scale. With this information, further research can be done to understand the correlation between attitudes towards religion and religious participation/exit. The authors for this project are Samantha Denbow, Katie Finlinson, Joseph Johnson, Tanner Dougherty, and Carley Grube. This information will be presented in a poster format.

Authors: Parker Graves, Landon Deru, Bruce Bailey. Mentors: Bruce Bailey. Insitution: Brigham Young University. Chronic illness such as strokes, heart disease and diabetes all rank among the leading causes of death in the United States. Recently, fasting has gained popularity as a means of preventing and treating chronic illness. PURPOSE: Fasting produces multiple beneficial physiological responses that have been shown to aid in chronic disease prevention, one of which is observed in relation to incretin hormones such as glucose-dependent insulin tropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1). These incretin hormones are released by the gut to augment the secretion of insulin to regulate postprandial glucose levels. During a fast, the decrease in incretin hormones, and resultant insulin levels can aid the body to regain insulin sensitivity. This can lead to more effective blood glucose management and chronic illness prevention. The purpose of the study was to determine the impact of an acute 24-hour fast started with either a high fat (HF) or high carbohydrate (HC) meal on plasma GIP and GLP-1. METHODS: Subjects were over the age of 55, had a BMI equal to or greater than 27, and had no diagnosed metabolic disorders or some other disqualifying medical issues. Using a randomized crossover design, each participant performed two 24-hour fasts. One fast beginning with a high carbohydrate meal and the other a high fat meal, both of equal calories. Venous blood draws were taken at 0, 1, 24, and 48 hours. RESULTS: GIP and GLP-1 (P < 0.001) were both elevated 1 hour after consuming the pre-fast meal in both conditions. In addition, both GIP (P = 0.0122) and GLP-1 (P = 0.0068) were higher in the high fat condition compared to the high carbohydrate condition at 1 hour. There were no significant differences between conditions for either GIP or GLP-1 at any other time point. CONCLUSION: As expected, both incretin hormones spiked postprandially. We did find that GIP and GLP-1 levels were significantly higher at 1-hour postprandial for the HF meal compared to the HC meal. This could give evidence to show how macronutrient levels can affect incretin secretion and alter sensitivity to insulin. However, the impact of the pre-fast meal on GLP-1 and GIP did not persist throughout the fast.

Authors: Leila Moore, Marin Farnsworth. Mentors: Tyson Harmon. Insitution: Brigham Young University. Background:Facial expressions are crucial for understanding human emotions in communication. Coding and quantifying these expressions, however, have often been subjective, leading to issues with reliability and consistency (Kring and Sloan, 2007). To address this, we have modified the Facial Expression Coding System (FACES) protocol (Kring and Sloan, 2007), which traditionally relies on subjective interpretation. Our modified protocol employs a more objective approach by quantifying facial expressions based on the counting of facial units (e.g., eyes, corners of the mouth, eyebrows, and teeth). Each intensity rating corresponds to a certain number of facial units. For example, an expression involving one facial unit would receive an intensity rating of one whereas an expression involving two units would receive an intensity rating of two. Multiple studies have found that women are more emotionally expressive than men overall when considering gestures, body language, facial expressions, and tone of voice (Ashmore, 1990; Brody & Hall, 1993; Hall, 1984). Rather than focusing on overall emotional expressiveness, though, the present study aims to understand how gender impacts the production of emotional facial expressions specifically. Method:The proposed study aims to compare the frequency and intensity of facial expressions produced by men versus women when recounting personal narratives. Participants. Video footage from participants with and without aphasia producing personal narrative discourse will be obtained for equal numbers of male and female participants. Samples will include two personal narratives in response to prompts from the interviewer. The first prompt is to speak of an experience the participant has had with illness/injury/stroke, and the second prompt is to talk about an important event that has happened in their life. Procedure. A modified FACES protocol will be used to code facial expressions in videos obtained from AphasiaBank. The coding process is conducted with the audio muted to help eliminate distractions. The modified FACES protocol includes specific, operationalized criteria for what qualifies as a facial expression. This protocol does not include coding non-facial gestures or eye movements. However, we do take into account the narrowing and widening of eyes in addition to instances when the eyes are opened or closed with intention. A critical question we ask during coding is whether the facial expression conveys emotional content. We have established standards for intensity ratings and will outline how we arrived at these standards, as well as the distinctions between intensity ratings one, two, three, and four. The analysis process includes establishing a baseline expression for each participant and coding for 20 minutes before taking a break to maintain energy and productivity and to ensure accuracy in data collection. Additionally, secondary coding and a final pass for gestalt ratings are performed to ensure comprehensive analysis.Data Analysis. To address our research questions, we will conduct a comprehensive analysis, focusing on our participants' facial expressiveness during personal narrative storytelling. The analysis will involve quantifying the frequency of facial expressions exhibited by the participants. Additionally, we will consider the valence of these expressions, distinguishing between positive and negative emotional content. We will also assess the intensity of facial expressions by counting the number of facial units engaged during each expression. This examination of facial data will allow us to discern not only the prevalence of expressions but also emotional content and intensity, ultimately providing a more in-depth understanding of the gender differences in non-verbal communication during personal narratives.Anticipated Results:Our study aims to explore if there is a statistically significant difference in the quantity, valence, and intensity of facial expressions between men and women. By using a standardized approach for measuring emotional facial expression production, we hope to shed light on the nuances of non-verbal communication during personal narratives and contribute to a better understanding of gender differences in emotional expression. Consistent with previous research, we anticipate that females will produce more frequent and intense emotional facial expressions than males (Ashmore, 1990; Brody & Hall, 1993; Hall, 1984).References:Ashmore, R. D. (1990). Sex, gender, and the individual. In L. A. Pervin (Ed.), Handbook of personality: Theory and research (pp. 486-526). New York: Guilford Press.Brody, L. R., & Hall, J. A. (1993). Gender and emotion. In M. Lewis & J. M. Haviland (Eds.), Handbook of emotions (pp. 447-460). New York: Guilford Press.Hall, J. A. (1984). Nonverbal sex differences: Communication accuracy and expressive style. Baltimore: Johns Hopkins University Press.Kring, A. M., & Sloan, D. M. (2007). The facial expression coding system (FACES): Development, validation, and utility. Psychological Assessment, 19(2), 210–224. https://doi.org/10.1037/1040-3590.19.2.210.

Authors: Korina Ziegler, Aaron McKenzie, Wesley Ziegler, Spencer Maxwell, Bryson Carrier, Charli Aguilar, Alexandra Routsis, Talon Thornton, Jae Bovell, Setareh Star Zarei, Devin Green, Amanda Hawkes, Jeffrey C Cowley, Merrill Funk, James Navalta, Marcus M Lawrence. Mentors: Marcus Lawrence. Insitution: Southern Utah University. ACSM has again determined that resistance training (RT) and outdoor activities are two of the top ten worldwide fitness trends for 2023. We previously found that RT outdoors had a significantly lower perception of effort (RPE) compared to indoor RT, despite no physiological differences in heart rate (HR) and energy expenditure (EE). However, no study has examined other feelings during RT in indoor or outdoor settings. PURPOSE: To determine how indoor or outdoor environments effect perceptions of fatigue and physical activity enjoyment following RT in recreationally resistance trained adults. METHODS: Twenty-three adult participants (n=10 female, n=13 male) completed this study. The Visual Analog Scale Fatigue (VAS-F) measured perceived fatigue and the Physical Activity Enjoyment Scale – Short Version (PACES-S) measured PA enjoyment, and both were measured at baseline and then immediately following an acute session of indoor or outdoor RT. HR was obtained from a chest strap (Polar H10) and EE from a Portable Metabolic Cart (COSMED K5). Randomly in indoor and outdoor settings, participants completed 4 supersets of the reverse lunge and shoulder press exercises using dumbbells at a light (2 sets) and moderately heavy (2 sets) intensity with 1 superset of 6 repetitions per exercise and 1 min rest between supersets. A paired T-test (for HR & EE comparisons) or one-way repeated measures ANOVA with Sidak post-hoc test (for VAS-F & PACES-S comparisons) were used to determine differences (p<0.05). RESULTS: No significant differences were observed between indoor and outdoor RT for the physiological variables of average HR (129.4±17.2 and 127.75±23.3 bpm, respectively, p=0.66) and EE (30.6±11.5 and 28.3±9.9 kcals, respectively, p=0.06). Perceived fatigue significantly (p<0.0001) increased from baseline (1.13±0.94 arbitrary units, AU’s) following indoor (4.54±1.91 AU’s) and outdoor (3.99±1.54 AU’s) RT, but no environmental differences (p=0.36) were observed. PA enjoyment was not significantly (p range: 0.27-0.93) different between baseline (18.73±1.83 AU’s) and following indoor (18.18±1.99 AU’s) or outdoor (18.36±1.99 AU’s) RT. CONCLUSION: In recreationally resistance trained adults, moderately heavy superset RT in indoor or outdoor settings does not alter perceived fatigue or physical activity enjoyment.

Authors: Ken Dixon, Jack Davis, DeLayney Anderson, Mackenzie Burr, Peyton Worley, Isaac Packer, Bridger Kearns, Jeffrey Okojie. Mentors: Jared Barrott. Insitution: Brigham Young University. IntroductionPersonalized cancer care requires molecular characterization of neoplasms. While the research community accepts frozen tissues as the gold standard analyte for molecular assays, the source of tissue for all testing of tumor tissue in clinical cancer care comes almost universally from formalin-fixed, paraffin-embedded tissue (FFPE). Specific to genomics assays, numerous studies have shown significant discordance in genetic information obtained from FFPE samples and cryopreserved samples. To explain the discordance between FFPE samples and cryopreserved samples, a head-to-head comparison between FFPE and cryopreserved tissues was performed to analyze the DNA yield, DNA purity, and DNA quality in terms of DNA length.MethodsHuman (n = 48) and murine tissues (n = 10) were processed by traditional formalin fixation and paraffin embedding or placed in cryovials containing HypoThermosol solution. 19 human-matched samples were included. These cryovials were cooled to -80°C slowly and stored in liquid nitrogen until the time of the study. DNA was extracted using the same protocol for both tissue types except that tissues embedded in paraffin were first dewaxed using a xylene substitute followed by a multistep rehydration protocol using ethanol and water. Samples were weighed and calibrated to have the same starting mass. After the column purification, samples were eluted in 20 µL and concentration and purity were measured on a Nanodrop. Purity was determined by calculating the 260/280 ratio. DNA fragment length was measured on an Agilent Fragment Microelectrophoresis Analyzer.ResultsGraded amounts of tumor tissue (5- >50 mg) were used to determine the lowest starting material needed to extract 40 ng/mg of DNA. The average for both sample types reached the minimal threshold of 40 ng/mg. However, 74% of FFPE specimens failed to meet the minimum 40 ng/mg, whereas only 21% were below the threshold in the cryopreserved samples (Figure 1). In the cryopreserved group, the average DNA yield was 222.1 ng/mg, whereas 52.8 ng/mg was obtained from FFPE tissue. For DNA purity in cryopreserved tissues, the 260/280 ratio range was 1.09-2.13 with a mean of 1.79. The 260/280 ratio range in FFPE tissues was 0.85-2.76 with a mean of 1.65 (Figure 2). The DNA Quality Number(DQN) is a measurement of DNA fragment length and the percentage that exceeds the threshold of 300 bp. For FFPE, the DQN was 4.4 compared to a DQN of 9.8 for the cryopreserved samples (Figure 3). Setting a higher threshold of DNA length to 40,000 bp and measuring the area under the curve (AUC), it was observed that cryopreserved samples were 9-fold higher in fragments greater than 40,000 bp (Figure 4).ConclusionsCryopreserved cancer tissue provides superior quality assurance measurements of DNA over FFFPE. Treatment decisions based on molecular results demand accuracy and validity. The pathology community should support efforts to cryopreserve cancer biospecimens in the clinical setting to provide valid molecular testing results. The automatic pickling of tumor specimens in formalin is no longer an acceptable default.

Authors: Ashley Allan, Mikayla Kimball, Noah Bezzant, Brent Feland, Josh Sponbeck. Mentors: Brent Feland. Insitution: Brigham Young University. BACKGROUND: Recent studies have shown that there are differences bilaterally in the cross sectional area of the patellar tendon for lead vs non lead extremities of athletes. Yet, little research can be found as to whether there is a difference that develops over one’s lifetime between the stiffness of the patellar tendons in the dominant vs non-dominant legs. Reliability has not yet been established for elastography in the patellar tendons, so we are continuously striving to gather more reliable data on shear-wave elastography of the patellar tendon. PURPOSE: The aim of this study was to assess whether there is a difference in the average patellar tendon stiffness as measured by ultrasound elastography using a pad vs no pad and differences between self reported non-dominant vs dominant knee of senior athletes over the age of 50. Dominance taken as reported in a modified KOOS (Knee Injury and Osteoarthritis Outcome Score) survey. METHODS: Data was collected from 15 active, senior aged volunteers at the Huntsman World Senior Games in St George, Utah, 2023. All subjects (mean age= 67.29 ± 6.26 yrs, height=175.44 ± 8.18 cm, weight=87.40 ± 12.21 kg) signed an approved consent and completed a modified KOOS survey. Following, they were seated on a table, with their backs against the wall directly behind them. They were seated so that their lower legs were hanging off of the table in a relaxed position. The patellar tendon was then imaged with a long axis view using ultrasound elastography. ANALYSIS: All data were analyzed using JMP ver16.2 with a repeated measured analysis of variance (ANOVA) to determine if differences existed between pad and no pad and between dominant and non- dominant legs. RESULTS AND CONCLUSION: There was a significant difference between (p=.0423) pad and no pad patellar tendon measurements, but no significant difference when comparing sides combined with pad and no pad, between dominant and non-dominant legs, although a trend for significance did exist, and we suspect that with more subjects analyzed, we will get more significance.

Authors: Logan McMaster, Joshua Sponbeck, Malorie Wilwand, A. Wayne Johnson. Mentors: A Wayne Johnson. Insitution: Brigham Young University. In the dynamic game of professional football, where split-second decisions and explosive movements often determine the outcome, players train all year round for optimal athletic performance. Paramount to this performance are the hamstring muscles including the semimembranosus (SM), semitendinosus (ST), and both the long and short head of the biceps femoris (BFLH and BFSH). They play pivotal roles in sprints, agility, jumping, and overall lower-body strength. Because of the critical nature of the hamstrings during functional activity, this study sought to determine which of these three muscles contributes the most to eccentric hamstring strength. Recognizing the correlation between muscle volume and muscle strength, this study compared maximal eccentric hamstring strength with the proportion of each isolated hamstring muscle's volume relative to the total hamstring muscle group volume. Doing so would identify which specific muscle contributes the most to eccentric hamstring strength. This study incorporated 74 male, collegiate football players representing all positions on the team. Maximal strength was assessed through Nordic hamstring curls and overall muscle volume was quantified using magnetic resonance imaging (MRI). Our results show no correlation (R^2<0.2) between maximal force output and proportional muscle volume for the ST, and the BFSH. Additionally, the correlations for these muscles were not statistically significant (P>0.05). The SM muscles had a weak negative correlation (R^2= -0.25) with eccentric hamstring strength that was statistically significant (P = 0.03). The BFLH proportional volume was positively weakly correlated (R^2= 0.22) with eccentric hamstring strength. This correlation was statistically non-significant (P = 0.06). This information shows that no hamstring muscle contributes more to eccentric hamstring strength than any other. Knowledge of this can be invaluable for clinicians and researchers to design comprehensive strength training programs that enhance the collective strength of the entire hamstring muscle group, ultimately optimizing the performance of football players.

Authors: Marcus M Lawrence, Merrill Funk, Jeffrey C Cowley, Amanda Hawkes, Aaron McKenzie, Alexandra Routsis, Wesley Ziegler, Talon Thornton, Spencer Maxwell, Korina Ziegler, James Navalta. Mentors: Marcus Lawrence. Insitution: Southern Utah University. American College of Sports Medicine has again found that wearable technology and resistance training (RT) are two of the top 5 fitness trends in 2023. Our lab recently found that the bicep-worn Polar Verity device was valid and reliable for measuring average and maximal heart rate (HR) during light intensity circuit RT. However, no study has examined other bicep worn devices during RT while also examining heavier intensities. PURPOSE: To determine the concurrent validity of identical Polar OH1 (x2) and Verity Sense (x2) bicep-worn devices in recording average and maximal HR following moderately heavy RT. METHODS: Twenty-one adult participants completed this study (n=10 female, n=11 male; age: 26.1±9.2 yrs; height: 171.3±9.4 cm; mass: 71.4±18.2 kg; RT experience: 5.7±4.9 yrs). The four bicep devices (Polar OH1 x2 and Polar Verity Sense x2) were worn along with the Polar H10 chest strap, criterion for HR. Participants completed 8 supersets of the reverse lunge and shoulder press exercises using dumbbells at a light (4 sets) and moderately heavy (4 sets) intensity with 1 superset of 6 repetitions per exercise (12 repetitions per superset) and 1 min rest between supersets. Data was analyzed for validity (Mean Absolute Percent Error [MAPE] and Lin’s Concordance Coefficient [CCC]), with predetermined thresholds of MAPE<10% and CCC>0.70. Paired t-tests were used to determine differences (p<0.05). RESULTS: For average or maximal HR, neither the Polar Verity Sense 1 (127.2±17.8 or 151.5±16.7bpm) or 2 (125.7±18.8 or 147.9±18.9bpm) or the Polar OH1 1 (128.7±18.5 or 154.5±18.1bpm) or 2 (129.5±18.2 or 156.4±17.4bpm) were significantly (p range: 0.14-0.97) different than the criterion (128.6±19.2 or 149.3±18.0bpm). However, the Polar Verity 1 and 2 were not considered valid for average HR (MAPE range:16.17-17.57%; CCC range: 0.07-0.13) or maximal HR (MAPE range: 11.60-13.33%; CCC range: 0.02-0.29). The Polar OH1 1 and 2 devices were not considered valid, either, for average HR (MAPE range: 17.22-17.25%; CCC range: 0.08-0.09) or maximal HR (MAPE range: 13.24-13.92%; CCC range: .024-0.27). CONCLUSION: Despite our lab previously finding the Polar Verity as valid for HR measurements during light intensity RT, the current bicep-worn devices should not be utilized during heavier intensity RT for accurate HR measurements. Individuals resistance training and utilizing bicep-worn devices for heart rate should use them cautiously.