Fine Arts

Authors: Spenser A Clark. Mentors: Acacia Overono. Insitution: Utah Valley University. It is not uncommon for a college student to see a list of learning objectives when skimming through a syllabus for a program or course. The goal of learning objectives is to set reasonable expectations for student learning and guide their study, as well as providing a framework for the development of teaching practices and assessment. Part of the inception of learning objectives as a means of organizing teaching and assessment in the classroom may be attributable to the Taxonomy of Educational Objectives, more commonly referred to as Bloom's Taxonomy. Another taxonomy similar to Bloom's, but much less well known, is the Structure of Observed Learning Outcome (SOLO) taxonomy. Bloom’s taxonomy organizes learning objectives into six categories: Remember, Understand, Apply, Analyze, Evaluate, and Create. The categories are structured as a cumulative hierarchy in which mastery of each level is assumed necessary for progressing to the next. The structure of Bloom’s is not derived from any specific learning theory, but a categorization of pre-existing learning objectives. Bloom’s therefore suffers from a lack of a strong theoretical foundation and may not accurately reflect how learning actually occurs. Additionally, although Bloom’s has been widely employed in the context of educational assessment, research has shown that it suffers from low interrater reliability and is inconsistent between educational contexts such as courses and topics. Unlike Bloom’s, SOLO is based upon Piaget’s Stage Development theory and categorizes learning into five distinct stages: Prestructural, Unistructural, Multistructrual, Relational, and Extended Abstract. SOLO does not suggest that learning is unidirectional or hierarchical. It also acknowledges internal cognitive processes rather than focusing solely on observable behaviors. Although very little research has investigated its reliability, it could be an attractive alternative to Bloom’s because it may more accurately reflect how learning occurs. The present work reviews the development and implementation of these two models and compares them.

Authors: Ezra Stein. Mentors: Nicole Dib. Insitution: Southern Utah University. In Simulacra and Simulation (1981), Jean Baudrillard lays out for stages through which a sign (an image or other representation of reality) becomes a simulacrum (a sign with no basis in reality). I argue that the followers of the Qanon conspiracy movement, as detailed in Mike Rothschild’s "The Storm is Upon Us" (2021), follow these four stages in their radicalization into the conspiracy. The first stage outlined by Baudrillard is when the image is a basic reflection of reality itself. We see this stage when Rothschild discusses how QAnon believers discuss the CIA. Adherents of QAnon will often point to the declassified MKULTRA documents released by the CIA for credibility, as this is something that was proven to have actually happened, the sign being a representation of actual reality. The second of Baudrillard’s stages is when a sign takes something from reality and twists and distorts it into something different than what it originally represented. An example of this in "The Storm is Upon Us" is seen in the way Q believers talk about adrenochrome, which is a real but harmless chemical compound, as an elixir of youth used by the elite. Stage three is when a sign claims to be based in reality but is actually based on nothing at all. The originator of QAnon, the man who goes by “Q,” fits within this stage. Rothschild lays out Q’s origins as an anonymous poster on the website 4chan who claimed to high-level military clearance. Q’s claims were supposedly based on real-life military intel, but were actually made up by an anonymous stranger on the internet. The fourth and final of Baudrillard’s stages is the simulacrum. This is when signs do not reflect anything in reality, but instead are based on other signs. The Storm is Upon Us shows that many QAnon beliefs, both major and minor, are based in pre-existing conspiracy theories like blood libel, flat earth, and global cabals that secretly control the world. Those who are completely indoctrinated into QAnon hold these beliefs that are based on other beliefs and are therefore existing in Baudrillard’s fourth stage of the precession of simulacra. My presentation will examine these four stages and demonstrate the overlap between this critical theory and the contemporary conspiracy movement that is taking up a considerable space in the American cultural imaginary.

Authors: Joseph Nielson, Ben Schooley. Mentors: Ben Schooley. Insitution: Brigham Young University. Chronic obstructive pulmonary disorder (COPD) affects an estimated 30 million Americans and is the third leading cause of death worldwide. A recent effort to curb deaths and hospitalizations involves remote patient monitoring (RPM). Of all possible monitoring parameters, spirometry presents itself as potentially accurate and helpful, but the exact effect and its size remain to be described. A systematic literature review is being performed to describe the current state and future opportunities in RPM systems that use remote spirometry for COPD patients. Only studies that specifically mention the use remote spirometry for monitoring COPD patients were included. The databases PubMed, Web of Science, Scopus, and EBSCO were searched in July and August 2023. Studies are being synthesized by tabulating the study sizes and designs, technological parameters, and interesting findings. Twenty-nine studies have been included spanning feasibility and pilot studies as well as one randomized clinical trial. Remote spirometry for COPD patients has generally been shown to be feasible. Many studies present systems in early stages of development that have yet to be tested thoroughly in large trials. It may be necessary to involve remote spirometry in more large-scale trials soon. Future studies could confirm the degree to which spirometry adds helpful information for monitoring COPD patients.

Authors: A. Kalani Brubaker. Mentors: Nathan Crane. Insitution: Brigham Young University. Binder Jetting is an exciting form of Additive Manufacturing in which a binding agent is selectively deposited on layers of powder to bind the powder together and create a 3D printed part. It is notable for its wide range of materials, energy efficiency, and built-in supports. Much research has been done on how to achieve desirable properties of binder-jetted parts and post-processing, but the fundamental principles of binder-powder interaction and the effects of changing printing parameters are still not fully understood. This research aims to increase that understanding. Each binder-jetted part begins by depositing individual binder droplets in a straight line, and the binder-powder droplets coalesce to form a single line. Adjacent lines are then printed to form a cross-section of the part, the next layer of powder is applied, and the process continues until a 3D part is printed. If the spacing between the droplets is too large or the inter-arrival time too short, the droplets form spheres on the surface of the powder (a phenomenon called balling), and a continuous line is not formed. This research determines how continuous line formation is affected by droplet spacing, inter-arrival time, and controlled misting of the powder bed prior to printing. To examine the effects of these parameters, lines were printed in dry and prewetted powder beds while varying the spacing and inter-arrival time between droplets. The beds were then examined using an optical microscope to determine whether continuous lines were formed. The results were recorded on a graph of inter-arrival time versus droplet spacing, and the “boundary line” for successful line formation was found for each bed. The results showed that the boundary on the prewetted data exhibited a different relationship than dry powder beds, suggesting that a different binder-powder principle may dominate after prewetting. The prewetting also shifted the boundary upwards, making it possible to form continuous lines at higher droplet spacings and shorter inter-arrival times. This increased ability to form continuous lines has the potential to significantly increase the throughput of binder-jetted parts.

Authors: Danyon Gedris, Paul Frandsen. Mentors: Paul Frandsen. Insitution: Brigham Young University. Whole genome assembly has rapidly improved as third-generation sequencing technology like PacBio HiFi and Oxford Nanopore (ONT) have bridged the gaps of complex genomes by providing high-accuracy, long read data. The improvements in these technologies have resulted in long average read lengths (>15 kbp) and sequence quality scores above 99% (>Q20). They are particularly well-suited to assembling long, repetitive regions of the genome. Current assembly techniques combine reads with identical sequences to form longer, continuous sections. In repetitive regions, this process tends to condense the repeated sequences into one shorter read, instead of preserving the continuous nature of the repeats. Long reads avoid this issue by sequencing repeats together in one continuous read. Heavy chain fibroin (h-fibroin), the gene that encodes for the primary silk protein in Trichoptera and Lepidoptera, is long (often >20 kbp) and repetitive. Recent work showed that PacBio HiFi sequencing provided higher quality assemblies of h-fibroin when compared to the last generation of ONT pores (R9.4.1) and chemistry despite having a shorter average read length. Recent advances in ONT chemistry and nanopores (R10.4.1) have led to higher quality scores, perhaps allowing successful assembly of this gene region. To better understand the advances in ONT sequencing and its ability to provide high-quality, continuous genome assemblies of complex organisms, we assess the quality of assemblies of the h-fibroin silk gene for the Trichoptera species, Arctopsyche grandis and Parapsyche elsis, using the newest ONT chemistry.

Authors: Kaerli Ringle. Mentors: Johnathan Chidester. Insitution: Southern Utah University. This presentation will discuss the way that music affects memory retention, memory recall, and how it can influence the memory of moments, moods, and emotions. The Public Broadcasting System produced an educational video about how listening to music boosts learning and memory. Research from Harvard noted that while neuro-imaging a patient, singing will help a patient during recovery with a brain injury or a stroke. This results because the left-brain region is majorly responsible for our speech function. Researchers also noted that because singing takes place in the right hemisphere, recovering stroke, Dementia and Alzheimer’s patients are able to regain their speech through singing and eventually being able to transition back into speaking. Having knowledge of how music affects the brain can help build great study habits and empower people to retain new information, recall old information, and have influence over their and other people’s emotions. This is important because music is around us all of the time, whether it is walking through the grocery store or playing through earbuds while studying, and if it has such power to influence these things, then why not use it to the fullest of its potential?

Authors: James Wade, Chris Paul. Mentors: Nathan Usevitch. Insitution: Brigham Young University. Soft Robotics offer significant advantages when completing certain tasks compared to other robots and methods. Compliance, adaptability, and energy efficiency are a few of the major advantages. On the moon, where safety, volume and versatility are of high importance, soft robotics may find an innovative niche. NASA’s BIG Idea Challenge for 2024 incentivizes creating new innovative soft robot designs. Our research group is building our facility mentor, Nathan Usevitch’s, work at Stanford University. Doctor Usevitch and his cohort developed an octahedral, inflated robot composed of multiple tubes folded into triangular shapes in a truss-like structure. Each triangle consisted of a long inflatable tube and 3 motorized modules at the nodes of the triangle. The motorized nodes (consisting of a DC motor and rollers) roll along the length of the tube, lengthening one edge of the triangle while shortening the other. By attaching multiple triangles to each other in geometric shapes, flexible structures are created that can adapt their shape to the desired need. Because this system has a constant inflated volume, no external air compressor is necessary, allowing for untethered movement. The bulk of the mechanical work is performed by electric motors, providing a higher energy efficiency than a system of repeated inflation. Furthermore, this design is compactly stored when deflated, optimal for transport into space.To build on this design and optimize it for use on the moon, our research group will make the following changes and improvements:- Developing the physical prototype. This will enable robust and dynamic movement and the support of large loads- Develop a simulation tool that allows us to explore different configurations of the robot for different tasks- Develop a joint system that allows an astronaut to quickly reconfigure the triangles into different structures based on the required task - Develop a control approach that accounts for the underdamped dynamics of large inflatable structuresThrough our research, we hope to expand the possibilities of soft robotics. We are synthesizing the advantages of traditional robotics (robustness, untethered motion) with the unique strengths of soft robotics (flexibility, adaptability, cost efficiency).

Authors: Sebastian Partida. Mentors: Jamie Spinney. Insitution: Southern Utah University. Lighting levels have an important role in influencing the ambiance and functionality of various land uses. To achieve specified goals while optimizing energy efficiency and sustainability, lighting intensity must be balanced. The purpose of this study was to compare light intensity for different land uses (e.g., residential, commercial, industrial, and recreational). This study measured light intensity (i.e., brightness) for a random sample of different land uses using a digital light meter within Cedar City, UT. GPS coordinates were also acquired in order to map the brightness data. As expected, the results revealed that residential land uses were the darkest, whereas commercial and industrial land uses were the brightest. The findings indicate communities have to maintain a balance between creating a warm and pleasant atmosphere and ensuring appropriate illumination for safety and security purposes. Energy-efficient LED fixtures and smart lighting systems, for example, can provide adjustable and programmable illumination settings that meet both aesthetic and safety requirements.

Authors: Erika Frandsen. Mentors: C. Riley Nelson. Insitution: Brigham Young University. The Timpanogos Cave System consists of three caves in American Fork, Utah. In 2003, the National Park Service (NPS) commissioned Dr. Riley Nelson and laboratory students to conduct a survey of the diversity and abundance of terrestrial arthropod species found within the caves. Over the two years they sampled in Timpanogos Caves, they identified 55 species of macroinvertebrates in an unpublished report.In the 20 years since the last sampling, many environmental factors could’ve changed in these caves, contributing to an overall decrease in both abundance and biodiversity of fauna. Therefore, we repeated the sampling done by the Nelson lab in 2003-2004 for the duration of May 2023-October 2023 to compare the two time periods’ macroinvertebrate communities.The six taxonomic families found most abundantly in 2003-2004 continue to be the most abundant families found in 2023. Additionally, a new taxon of cave beetle Rhadine sp. not found 20 years ago was discovered early in our sampling of 2023. Despite the increase in visitation over the past 20 years, at this stage in the analysis, the macroinvertebrate community within the caves seems to have almost no loss of diversity. Additionally, the appearance of new taxa indicates that there is still more life and diversity within the caves left to discover.

Authors: Kylee Fernandez. Mentors: Gennie L Parkman. Insitution: University of Utah. Evaluating the role of LRRC1 in melanoma growth and metastasisThe development of brain metastases is a critical problem in Utah, regardless of the novel therapies created for melanoma. The melanoma mortality rates in Utah are 31% higher than the national average and the melanoma incidences are 80% higher. To lower these incidences, our research is aimed at investigating targets in melanoma that play a crucial role in brain metastasis and can be identified as therapeutic interventions.LRRC1 is a gene known as Leucine Rich Repeat Containing 1. LRRC1 is involved in early-stage adipocytic differentiation. Altered gene and protein expression of LRRC1 is observed in melanoma potentially due to the vital role LRRC1 plays in adipose stem cells that differentiate by adopting the phenotypic, biochemical, and functional properties of more terminally differentiated cells. To evaluate the effect of LRRC1 on melanoma growth and metastasis, we are using an established autochthonous in vitro and in vivo model of melanoma based on the RCAS/TVA avian retroviral system. The results of this study will have tremendous potential to contribute to the understanding of the genetics of this heterogeneous disease.

Authors: Nathaniel Call. Mentors: Chelsea Romney. Insitution: Brigham Young University. New college students may experience stressors like difficult academic work, new social environments, and living on their own for the first time. Students of color may experience additional stressors due to minority stress, experiences of racism, and unfamiliar social contexts. We collected self-reported depression, anxiety, and academic self-efficacy measures from 742 first-year college students. We found that students of color reported higher levels of depression, F(1, 733) = 11.04, p < .001, and anxiety F(1, 734) = 14.91, p < .001, compared to white students. Further, we found that white students reported higher academic self-efficacy, F(1, 742) = 9.97, p = .002, compared to students of color. This suggests that lower confidence in academic work is related to higher depression and anxiety in nonwhite students, r(661) = -.32, p < .001. Our study builds on previous research by providing a possible pathway through which students of color develop more depression and anxiety through lower academic self-efficacy compared to white students.

Authors: Nicholas Poll. Mentors: Moriel Zelikowsky. Insitution: University of Utah. Social interaction is one of the chief drivers of evolution and natural selection. Animal interactions shape the ecological world and have shaped the world’s evolutionary timeline. Of the many types of animal interactions, one of the most influential to behavior and development is aggression. Many animals' aggressive behaviors and mechanisms are so distinct that their origins can be traced back to specific selective pressures. These pressures range from reproductive competition to predator-prey interactions and have apparent effects on many species’ developments. There are many stressors that can increase aggressive tendencies found in mammals, yet it’s clear that the neural bases that connect these stressors and their behavioral responses are understudied. The targeted brain region in this project is the bed nucleus of the stria terminalis (BNST). The BNST is a neurochemically diverse node of the extended amygdala that has been implicated in mediating stress and sensory information. It anatomically acts as a relay station between a number of different structures including the hypothalamus and amygdala. The BNST has been implicated to have a role in stressor-induced aggressive behaviors; however, the neural mechanisms that connect this region and these behaviors are relatively understudied. Corticotropin-releasing Hormone (CRH) in mammals displays upregulation following the introduction of a stressor and has been found to mediate aggression and anxiety. Similarly, tachykinin-expressing neurons have been shown to have a role in mediating aggression in mammals. While CRH and tachykinin’s link to anxiety and aggression is well supported, it is not clear to what extent it has on BNST. This project aims to provide data to support the role that CRH and Tachykinin 2 (Tac2) in the BNST have in mediating aggression in mice through optogenetic stimulation.

Authors: Jared Carter. Mentors: Jeff Tessem. Insitution: Brigham Young University. Type 2 diabetes mellitus (T2D) is a chronic condition affecting nearly half a billion people worldwide. Symptoms of T2D include impaired glucose tolerance, decreased insulin secretion and significant weight gain. While the symptoms of T2D are well-documented, the underlying pathology remains unclear. Recent research has indicated the critical role of the nuclear receptor Nr4a3 in the development of glucose intolerance and weight gain. In individuals with T2D, the Nr4a3 promoter is hypermethylated, leading to decreased Nr4a3 expression. Elucidating the role of Nr4a3 in mitochondrial respiration in adipose will help define the mechanism of T2D onset and treatment. I studied mice with full body knockout (KO) for Nr4a3. These mice exhibited T2D-like symptoms, including impaired glucose tolerance, reduced insulin secretion and increased adiposity. I measured mitochondrial respiration in muscle, liver, kidney, and adipose tissue, with impaired respiration only observed in adipose tissue. This impairment in adipose tissue respiration correlated with an increase in the size of all adipose deposits, larger adipocytes, and expanded lipid droplets. Intriguingly, the analysis of electron transport chain and tricarboxylic acid complex proteins revealed no significant differences compared to control samples. Instead, the change in respiration was attributed to a reduction in active DRP1 protein, responsible for mitochondrial fission and maintenance. These findings have important implications for our understanding of T2D and its potential treatment strategies, which will be discussed in more detail.

Authors: Dallin Oyler, Ana Alfaro, Kailee Myxter, Ben Brooks, Amanda Brooks. Mentors: Jessica Pullan. Insitution: Southern Utah University. Nausea and vomiting are common complications that occur in 70% of pregnancies. Hyperemesis Gravidarum (HG) is the most severe form of these symptoms and is estimated to be prevalent in 0.3-2% of pregnancies. Due to the fetal and maternal morbidity associated with HG, identifying the cause and treatment options for these women is a critical task in obstetrics. Research regarding the etiology of HG has been fairly recent and is still ongoing, however, evidence had directed to a positive correlation between increased levels of the serum protein GDF15 and HG symptoms. We hypothesize that polymorphisms in both maternal and fetal DNA plays a role in the upregulated GDF-15 seen in mothers experiencing symptoms of HG during pregnancy. The DNA of 2 mothers and their corresponding children were sequenced and analyzed. The DNA was obtained through buccal swabs from the epithelial cells of the inner cheek, and then purified and ran through PCR. We employed 3 distinct primers that correspond to mutations in the genome that account for the elevated levels of circulating GDF-15 in the mother. As of current, the mother-daughter DNA is still under analysis for single-nucleotide polymorphisms, however recently published literature has suggested results similar to our hypothesis. M. Fejzo et al. shows that upregulated serum GDF-15 is primarily of fetal origin, and that maternal sensitivity to GDF15 increases the risk for developing HG. Additionally, the DNA coding variant GDF15 C211G was shown to elevate the risk of HG

Authors: Auriana Dunn. Mentors: Kathryn Sokolowski. Insitution: University of Utah. Reconstructing the diet of extinct animals can help us understand how living species are suited to their environments, which can inform paleoecological and zooarchaeological research as well as modern species conservation. Seasonal variation in animals’ diets can further be used to reconstruct seasonality patterns in the past and understand how animals may vary their diet under future climate change. However, determining the diet of extinct species is challenging, and detecting seasonal changes in diet is even more so. Dental microwear texture analysis (DMTA) can be a tool to solve this problem. DMTA analyzes dental microwear by measuring the pits and scratches on teeth to determine what an animal consumed in the days and weeks before it died. This project examines the DMTA of modern African Bovidae species to determine if seasonal dietary changes can be observed. If a difference can be found in the dental microwear of specimens that died during different seasons, seasonal changes in diet can be inferred. Here, I gathered dental microwear data from 407 specimens across five modern studies and matched the data to the museum specimens’ date of death to determine whether it died in a wet or dry season. The analysis found that four of the Bovidae species examined in our study showed evidence of seasonal diet shifts in their DMTA data. Dental microwear can be useful in determining seasonal diets and season of death for specimens. This method can be applied to extinct Bovidae species to understand ancient ecosystems and the evolution of Bovidae dietary strategies.

Authors: Justin Webb. Mentors: Jeffrey Edwards. Insitution: Brigham Young University. Understanding the mechanisms underlying depotentiation in the context of previously induced long-term potentiation (LTP) is crucial for unraveling the processes of memory consolidation and forgetting. Our research is focused on synaptic plasticity in the hippocampus, the primary brain region responsible for mediating learning and memory. Using electrophysiology and neuropharmacological techniques, our goal is to elucidate the cellular mechanisms behind depotentiation, a reversal of the increase in postsynaptic response and overall synaptic strength characteristic of LTP. By doing this research, we hope to both fill existing gaps and resolve conflicting views about the processes and receptors involved in depotentiation. Experiments were performed on hippocampal brain slices of young mice. We induced LTP in hippocampal CA1 neurons using high-frequency electrical stimulation, and then elicited depotentiation in the same neurons via low-frequency electrical stimulation. This creates a cellular event analogous to learning and subsequently forgetting a new memory trace. Our preliminary findings show that depotentiation still occurs in the presence of MPEP, a metabotropic glutamate receptor (mGluR5) antagonist, suggesting that the depotentiation mechanism is independent of mGluR5. Additionally, because female rodent models have largely been ignored in prior electrophysiology experiments involving LTP and depotentiation, we investigated and observed gender-related differences, which show female mice exhibiting more pronounced depotentiation than their male counterparts. Building on these findings, our research will continue to identify gender-related differences in both juvenile and adolescent mice, as well as explore the role of NMDA receptors on depotentiation. We will also extend the temporal gap between LTP induction and depotentiation to explore the impact of early-phase versus late-phase LTP on subsequent depotentiation. This research aims to shed light on the intricate mechanisms of synaptic plasticity and its implications for learning, memory, and potential therapeutic modulation of these processes in the context of conditions like Alzheimer's disease, post-traumatic stress disorder (PTSD), and other disorders of learning and memory.

Authors: Zachary Flynn, Britton Davis, Josh Nicholls, Bryson Mumford. Mentors: Joseph Price. Insitution: Brigham Young University. The United States has massive amounts of public data and land ownership stretching back to the early homesteading period of the American West. This data has been of limited use for economic research because it only includes the name of the ownership, thus making it impossible to study racial and gender differences in land on ownership. We develop an innovative method for linking individual land records to US census records. Our key innovation is that we start by linking individuals with unique names that we can make from land record to census record based on name and town. We then use the geo-coordinates in the land records and the proximity of households on the census sheets to link other individuals that have more common names. This two-step process allows us to link 36% of a sample of people in the land records in Colorado to a census record between 1880 and 1940. This new linked data will be a valuable resource for individuals doing research on the development of the American economy, and also people doing genealogical research.

Authors: Cooper Bell-Hunley, Ming Yu, Emily Heider. Mentors: Emily Heider. Insitution: Utah Valley University. Green chemistry involves the redesign of chemical processes and products to reduce hazards to the environment and human health. Reconsidering the teaching curriculum with a view to embrace green chemistry can reduce costs in waste disposal and has the potential to improve accessibility. This research involves the redesign of qualitative analysis general chemistry experiment to eliminate the use of teratogenic mercury (Hg) and neurotoxic lead (Pb). Making this change creates a more inclusive laboratory since expectant mothers should not be exposed to mercury and therefore some students did not feel safe completing the previous version of the experiment. With any change in curriculum, it is important to consider the impact on learning outcomes that students can achieve. This research details the assessment of learning outcomes with a quasi-experimental design. Instructors in multiple general chemistry labs selected the qualitative analysis experiment (with or without Hg and Pb) and learning outcomes achieved were assessed following the experiment.

Authors: G. Colton Gardner, Lynasi Gapelu, Sohom Mookherjee, Sihem Boudina, J. David Symons. Mentors: J. David Symons. Insitution: University of Utah. With the prevalence of cardiovascular disease increasing world wide and its precise mechanisms remaining elusive, the need for novel treatments is urgent. Autophagy is a conserved process whereby dysregulated intracellular proteins are escorted to the lysosome for recycling. Emerging evidence indicates that autophagy is important in maintaining endothelial cell (EC) proteostasis and function. My laboratory showed that autophagy repression in adult mice ECs produces arterial dysfunction, and indexes of autophagy are repressed in ECs of older mice and humans which display concurrent EC dysfunction. Sequestosome 1 (p62) tethers proteins and enables their entry into the autophagic pathway. The contribution from EC p62 to cardiovascular and metabolic function is unknown and experiments are needed to investigate this. Thus, p62 will be depleted from ECs of adult male and female mice (p62iEC-/-) via tamoxifen regimen, and results will be compared to animals wherein EC p62 is intact (CON). The efficacy and specificity of EC p62 depletion will be evaluated in ECs and vascular smooth muscle by qPCR and immunoblotting. Three adjacent 1 mm thoracic aorta sections will be obtained 14 days after the tamoxifen regimen. The central aorta section will be used to measure intrinsic mechanical stiffness by wire myography, with the other two used to find lumen diameter and intimal medial thickness. These measurements will help create Stress vs. Strain curves and the Elastic Modulus, which will be compared between the groups to show the differences caused by p62 depletion. We expect p62 depletion from ECs to increase intrinsic aortic stiffness.

Authors: Seth Cannon. Mentors: Joe Price. Insitution: Brigham Young University. We use the occupation strings in the full-count US census files from 1880-1940 to create a new dataset of everyone during this time period that listed their occupation as a clergy (or related term). We use this data to identify the number of clergy located in each town in the United States in each census year. We find that towns with at least one person that identifies as clergy in the census record experience 5.4% greater population growth by the next census compared to towns without any clergy. This result is robust to various ways to control for the population and other characteristics of the town. We also focus on just the set of towns that had only one person in the census who identified as clergy. When we restrict the dataset to these towns, we find that the towns for whom that single clergy member died before the next census experienced an average population loss of 2.8%, while towns that didn’t have their clergy die experienced a population growth of 12.5%. These results highlight the ways in which access to local religious resources can help promote economic growth in small communities.

Authors: Cate Bateman, Carson Veazie, . Sydney Sandall. Mentors: Matt Ogburn. Insitution: Southern Utah University. Western Utah is home to two distinct species of oak, Gambel oak (Quercus gambelii) and Dixie live oak (Quercus turbinella), which can hybridize to form an intermediate species that has been named Quercus undulata. In spite of previous research on these shrubs, we are still unsure of how the hybrid has survived in small isolated locations well outside of the range of one parent species (Dixie live oak, which is restricted in Utah to Washington, Kane, and San Juan Counties). Our primary objective was to perform an ecological niche modeling assessment of Q. undulata within the Three Peaks Recreation Area. Our approach included collecting plot-level data to determine abiotic and biotic niche factors correlating with the presence/absence of Q. undulata, including slope, aspect, soil type, soil chemistry, vegetation cover, and other associated woody plant species. Our results have shown that Q. undulata populations have higher concentrations in south-facing slopes, potentially reflecting the climatic preferences of its Dixie live oak parent. Results also showed that both focal species, Q. undulata and Q. gambelii, prefer areas with shallow soil and rocky outcrops. We plan to continue data collection and our analysis of soil type and chemistry. These results will help us to better understand the evolutionary history and ecological requirements of this fascinating native Utah plant.

Authors: Abigail Cheever, Chloe Kang, Hunter Lindsay, Mackenzie Hansen, Kim O'Neill, K Scott Weber. Mentors: K Scott Weber, Kim O'Neill. Insitution: Brigham Young University. Chimeric Antigen Receptor (CAR) T cell therapy is a modern technology that has become a widely accepted treatment for blood cancers such as lymphoma and leukemia. Recent studies have proven the successful application of CAR T cell therapies in autoimmune diseases as well. Graves’ Disease (GD) is an autoimmune disease that affects approximately 1 in 100 Americans and is the most common cause of hyperthyroidism. GD is mediated by anti-thyroid stimulating hormone receptor (TSHR) antibodies produced by autoreactive B cells. Our hypothesis is that a curative treatment for GD can be created by designing a CAR T cell that specifically targets the autoreactive B cells in GD, by using TSHR as a binding domain to act as bait for the anti-TSHR B cells. We selected TSHR epitopes for the binding domain of the CAAR T cell, and our anti-TSHR antibodies bound significantly to our engineered CAAR T cells. A target B cell line with anti-TSHR B cell receptors was engineered using the Nalm6 B cell line. Using primary human CAAR T cells, activation and cytotoxicity assays against anti-TSHR B cells shows that CAAR T cell therapy is an effective and promising method to treat antibody mediated autoimmune diseases like GD.

Authors: Jacob Armstrong, Tyler Standifird. Mentors: Tyler Standifird. Insitution: Utah Valley University. Lower Limb Asymmetries in Unilateral Jump Mechanics as Possible Risk Factor for Injury in Collegiate WrestlersJacob Armstrong and Tyler Standifird 1Utah Valley University, Orem, Ut. Injuries impose a multi-faceted burden on athletes, coaches, and sporting organizations, encompassing physical, financial, and psychological ramifications. Determining risk factors and identifying them in athletes has been a widespread goal amongst researchers and practitioners in attempts to reduce injuries. Investigating Limb asymmetries, or limb imbalances, has been a common approach in identifying such factors but has shown inconsistent correlations with injuries (Melony, 2018). Moreover, much of the current research investigates asymmetries of outward limb performance (i.e., Strength, flexibility, jump performance etc.) and not asymmetries of internal joint mechanics. Identifying relationships between internal joint asymmetries and lower limb injuries can further aid coaches, practitioners and sporting organizations in effectively identifying injury risks. Purpose: The purpose of this study was to investigate internal joint asymmetries of the lower limbs during unilateral jump testing and their association with injuries in collegiate wrestlers. Methods: 23 healthy Division 1 NCAA collegiate wrestlers performed single leg depth jumps from a 12-inch platform unto force plates (Bertec Inc 1000 hz). Additionally, markerless motion capture technology was used to collect video data using Qualisys Mocap cameras (Qualisys North America, Inc. Buffalo Grove, Ill). Imagery was then processed in Theia (Theia Markerless, Inc. Kingston, Ontario) to produce 3d joint locations for all the joints of the lower extremity. Each wrestler performed 3 jumps with the left leg and then 3 with the right leg for a total of 6 jump attempts. 7 total variables were calculated and recorded in Visual 3D software (Dolomite Enterprises, LLC. Tallahassee, FL) for each wrestler and leg: drop max vertical force, vertical loading rate on drop, peak knee adduction angle, peak knee abduction moment, peak knee adduction moment, peak knee flexion angle, peak knee extension moment. Averages, standard deviations and percent differences were also calculated and compared between limbs. Injury history data was also collected from each participant from the year 2021 - present day.Results: Averaging found asymmetries in peak knee adduction angle with the left knee being greater than the right by 4.9 degrees (p=0.001), peak knee abduction moment with the right knee being greater than the left by .95 newton meters (p=0.001), and peak knee adduction moment with the left knee being greater than the right by .44 newton meters (p=0.001). All significant asymmetries were found in the frontal plane. No significant asymmetries were found in the sagittal plane. Injury history report found a total of 24 seperate lower limb injuries within the described timeframe. 16 associated with the right limb and 8 associated with the left. A total of 5 ACL injuries, 8 MCL injuries, 5 LCL Injuries, 3 meniscus injuries, and 3 ankle injuries. Conclusion: Asymmetry trends in collegiate wrestlers potentially indicate that internal joint asymmetry is specific to sport or activity. Also, internal joint asymmetry could be a potential indicator for training protocols for overall better movement and health within the studied population. Asymmetrical values of the peak knee adduction angle, peak knee abduction moment, and peak knee adduction moment combined with the frequency of knee injuries of the participants indicate possible positive correlations between the magnitude of asymmetry discovered and injury risk.

Authors: Naavah Craze, crazen@byu.edu . Mentors: John Chaston. Insitution: Brigham Young University. Wolbachia are maternally inherited bacteria found in the reproductive organs of many arthropod species. These parasitic bacteria are influential in the reproductive fitness of the host and can affect a population’s male-to-female ratio. Wolbachia can only be cultured in living cells and current methods for inoculating hosts with this bacteria have low success rates. These factors make these bacteria difficult to study in lab environments. In this project, we test a pricking method for inoculating Drosophila melanogaster with Wolbachia. In this method, axenic, or bacteria-free, female flies will be pricked in the abdomen with a Wolbachia-coated needle. Eggs from these flies will be collected from 3 to 5 days after pricking and will be incubated. The offspring will be tested using gel electrophoresis to see if inoculation was successful. If successful, this method can be used as a simple and efficient way to introduce Wolbachia into Drosophila melanogaster. These inoculated flies can then be used in experiments to study the relationship between the bacteria and the host organism.

Authors: Lillian Davis, Paul B Frandsen. Mentors: Paul B. Frandsen. Insitution: Brigham Young University. Insects of the order Embioptera (webspinners) typically live in colonies that can cover the ground of entire fields with their silk-constructed galleries, providing them with camouflage from predators and cover from the rain. Uniquely amongst silk-producing insects, webspinners possess silk glands in their forelimbs and, like spiders, retain the ability to produce silk throughout their life history. They typically live together in semi-social colonies, feeding off of decaying plant matter. Previous studies have been limited to morphological descriptions of species, testing various qualities of their silk, and a few molecular studies based on short DNA sequences. Here we present the genome of Apostonia ceylonica, a webspinner species from India, providing the first embiopteran genome assembly, opening the door to a multitude of analyses. At a haploid size of approximately 3.1 gigabases, their genome is significantly larger than previous estimates, containing a significant number of repeating segments. This data provides valuable information for future studies into the genes that control silk production, information for identifying this species through meta-barcoding, and data that could improve phylogenetic trees. By evaluating the effect of repetitive elements on their oversized genomes, we gain insights into the evolutionary history of Apostonia ceylonica.

Authors: Lauren Heath, John Chaston. Mentors: John Chaston. Insitution: Brigham Young University. Mounting evidence shows that gut microbiota disruption is related to several physiological processes and disease development in mammals; however, the mechanisms by which these processes take place are not fully understood due to the complexity of the mammalian gut microbiome. Studying the fruit fly, Drosophila Melanogaster proves to be a valuable model for understanding the gut microbiome on a smaller scale. Previous research has identified microbiota disruption alters the fly’s ability to cope with thermal stress and causes slower chill coma recovery times. Chill coma recovery is an important survival trait and stress response in Drosophila flies. Microbiota may affect the ability of hosts to tolerate stressful situations. The purpose of this project is to determine the effect of different bacterial strains on the mechanisms of chill coma recovery through a metagenome analysis of different bacterial strains. I hypothesize that certain microbe genes will increase chill coma recovery times. To test this assumption, I will mono-associate by flooding different groups of eggs with a microbe wash and infusing their food with the same bacteria strain. After cultivation, a chill coma will be induced by placing mature adult flies in empty vials on ice for three hours, and then the time for each individual to stand up after their transfer to room temperature will be recorded. Subsequently, I will assess cold tolerance, and evaluate the rates of recovery. With this, we can further identify the mechanisms of the chill coma recovery process by pinpointing specific proteins necessary for this process through a metagenome-wide association approach (MGWA) and gene analysis. If I observe no effect of the bacterial strains on chill coma recovery times, I can test different mixes of microbes to determine if improved recovery rates are a side effect of global metabolic changes caused by decreased biodiversity in the gut of drosophila flies. Studying the metabolic processes of Drosophila allows us to unravel further the mechanisms by which gut microbes affect the fitness and health of their host. My findings may be extrapolated to further understand the human gut microbiome.

Authors: Hazel Ticas, Daniel Scott. Mentors: Daniel Scott. Insitution: Utah Valley University. The COVID-19 pandemic’s results, though highly publicized throughout its early years, fell out of the media’s attention over time. Despite the lower volume of media coverage, the effects of the pandemic remain ongoing. In an effort to maintain accurate information about the most current impacts of COVID-19, this study will review relative COVID-19 case amounts compared to population density for four populations during the omicron peak between the years of 2021 and 2022.. The four populations are: counties in New York, United States; counties in California, United States; a collection of the largest counties from the other 48 United States, and the states in Sweden. The population density for each of the four populations was corrected using Google Maps in order to eliminate areas that didn’t have any residents, but were part of the county or state boundaries. Boundaries have been defined as anything thats stops the spread of COVID-19, said boundary can be geographical or self-isolation. We aim to analyze the data trends of how Omicron spreads in relation to population density and boundary effects by comparing the ratio of virus spreadability to boundaries on each population. The goal is to create a model that can be applied to COVID-19.

Authors: Tiba Hasan, Elise Larsen, McKell Gillins. Mentors: Jodie Lopez. Insitution: Utah Valley University. AbstractThis paper discusses studies that have researched the most effective forms of teeth whitening and the mechanism behind it. Studies discuss the oxidation of double bonds which in turn lighten the chromogens impacting the overall coloration of dentition. The three main forms of treatment that were considered in these studies include in-office treatment, at home treatment and OTC products. At home overnight bleaching proved to have the best results for both immediate and post 10 week results. At-home treatment under dentist’s care involves lower concentration of hydrogen peroxide or carbamide peroxide ranging from three to ten percent thus it takes longer time than in-office treatment (Leh, B. 2018). Factors such as food intake, beverages, smoking, and oral hygiene care can all impact the efficiency of bleaching methods. While some toothpaste brands, like charcoal toothpaste, claim to whiten teeth, research suggests that these abrasive toothpastes may lead to enamel wear. Furthermore, studies have compared the effects of chemical and herbal toothpaste and found that both can produce similar whitening effects. However, chemical products tend to be more harmful compared to herbal alternatives As dental esthetics have become increasingly important to western populations, more whitening products are readily available for consumer use. Studies warn about the adverse risks and effects of dental bleaching to be aware of for both the consumer and those applying or prescribing bleaching methods, therefore it is important for hygienists and others in the dental field to become educated and aware of the increasing bleaching product selection.

Authors: Kirsten Sanders. Mentors: Ruth Kerry. Insitution: Brigham Young University. The Great Salt Lake (GSL) found in the Salt Lake Valley is a remnant of Lake Bonneville. In the 1980s the GSL reached record high water levels. But by 2022, the lake has been drying up and reached record-low water levels. Previous research using seven years of Purple Air Network data has shown that particulate air pollution levels, PM 2.5 and PM 10, are worse within 10km of the Great Salt Lake. Other research investigating the distribution of toxic heavy metals (Copper, Arsenic, Lead, and Zinc) in topsoil samples from throughout the Salt Lake Valley showed that some of the highest levels of Copper and Arsenic pollution were found near Saltair among dried sediments on the shoreline of the Great Salt Lake. This study involved collecting numerous samples from the dried shoreline of the Great Salt Lake and analyzing them for their heavy metal contents using Xray Fluorescence. The heavy metals were then mapped and linked to sediment particle sizes and pH to indicate where the most toxic sediments are located, and where the sediments that are most easily picked up by the wind are located. The maps of heavy metals will be used with our analysis of the Purple Air Network and wind rose data from our previous study to show where toxic dust clouds are most likely to be an issue to the population. Links with 2016 Asthma ER visits and Hospitalizations will also be investigated.

Authors: Eva Driggs, Megan Cahoon, Seth Bybee. Mentors: Seth Bybee. Insitution: Brigham Young University. Odonates (dragonflies and damselflies) exhibit diverse coloration. These vibrant colors are important for species recognition, camouflage, and mate recognition. Adult odonates emerge in late spring, mate and reproduce, and die by fall. As color and seasonality are both important aspects of the adult stage in odonates, we pose the question: Does the odonate community color composition change over the season?We hypothesize that different colors within the odonate community will peak in abundance at different times in the season. We conducted population estimates weekly at a local park in Provo and analyzed data from Utah, the Great Basin, and the West Coast (GBIF). From this data, we found that generally, blue odonates have the highest abundance throughout the season, but red odonate populations persist later in the season while blue populations decline which may be due to seasonal changes in heat and light.

Authors: Britt Wyatt, Alyssa Habel, Jess Cusick, Heath Ogden, Josh Premo. Mentors: Dr.Britt Wyatt. Insitution: Utah Valley University. Vaccines are crucial to maintaining healthy communities as they provide immunological protection against a variety of infections (Geoghegan, S. et al. 2020). By providing immunity, vaccines prevent serious illnesses, reduce disease severity, and protect communities. However, in 2019 the World Health Organization (WHO) declared vaccine hesitancy (resistance to getting vaccinated) as one of the top 10 dangers to global health (Dubé, Ève, et al. 2021). Previous studies have shown that certain communities are more vaccine hesitant than others. For example, individuals who are religious, conservative, Black, and women have been found to be more vaccine hesitant than other individuals (Savoia, E. et al., 2021) (Gowda C. & Dempsey, A., 2013). Therefore, we wanted to investigate vaccine hesitancy at an open enrollment institution that serves a unique student population where ~70% of students are members of The Church of Jesus Christ of Latter-day Saints and ~30% are non-traditional (over the age of 24) (UVU IR, 2023). Compared to the overall 1.6% of individuals in the United States who are members of The Church of Jesus Christ of Latter-day Saints (Pew Research Center, 2023), the identities of the students attending the open enrollment institution are vastly underrepresented in education research that has been conducted at other institutions. To investigate vaccine hesitancy, a survey was given to 1,444 students in the beginning of the Fall 2023 semester asking questions around vaccine safety and support. Preliminary results indicate that 70% of non-religious students will choose to be vaccinated against known and new diseases compared to 37% of religious students. 53% of non-religious students think that vaccine mandates are appropriate compared to 25% of religious students. These results indicate more vaccine hesitancy with religious students compared to non-religious students. As religious students make up most of the student population, results can help inform instructors and/or other stakeholders on scientific literacy topics to support in the academic setting. Lastly, further investigation on the intersectionality of identities will be explored such as gender and religion as women make roughly 80% of health-related decisions in their family unit. If religious women are more likely to be vaccine hesitant, development of strategies to support those students in making evidence-based decisions could be a future aim.

Authors: Coleman Judd, Alexander Beagley. Mentors: Michelle Culumber. Insitution: Weber State University. Due to the overuse of antibiotics in industrial, agricultural, and medical settings, antibiotic-resistant bacteria are becoming more prevalent in various unique settings. We set out with the goal of identifying antibiotic-resistant bacteria from the Great Salt Lake waterfront. After isolating colonies on agar containing the antibiotic Tetracycline, we performed 16S rRNA gene sequencing and identified three separate Enterobacteriaceae species all exhibiting varying levels of antibiotic resistance. We performed an array of biochemical and physiological tests to further distinguish the Enterobacteriaceae species. Full spectrum biochemical tests indicate two near identical species followed by a third, novel species as both a phenotypic and biochemical outlier. Antibiotic resistant bacteria within the Great Salt Lake region present an ever-growing dilemma of clinical and environmental danger that accompanies antibiotic resistant microbes.

Authors: Cooper Johnson. Mentors: John Chaston. Insitution: Brigham Young University. The main goal of this project is to determine the relationships between microbes in the microbiota of Drosophila and the activity rate of Drosophila at low temperatures. Previous research has already determined that the microbiota leads to changes in fly activity levels at normal temperatures (Schretter, 2019). Studying the relationship at low temperatures with the method I will outline below will help us to have a more holistic view of how the bacteria affect fly locomotion and behavior. This holistic understanding can open doors for other projects that study if manipulation of the microbiota could have a specific desired effect on the fly’s behavior, and it can help us understand how the microbiota’s impacts are affected by drastic changes in temperature. In this experiment we seek to determine how each of 41 different strains of bacteria affects fly activity at temperatures of approximately 17 degrees celsius using several different metrics to measure both locomotion and social behaviors. These metrics would be collected simultaneously by placing flies in a chamber for Drosophila Video Tracking (DVT), which would use a camera and tracking software to record the above-mentioned metrics on fly activity. Using so many different metrics allows us to have a more holistic view of the flies’ activity and to be able to better determine exactly how the bacteria are affecting the flies. In order to connect the data with the microbiota, the flies would be separated into 41 different experimental groups and each group would be “mono-associated” with one of the 41 different above-mentioned bacterial strains. Mono-association means that each of the fly eggs would be bleached, and then divided in groups, with each group being grown in an environment containing a specific type of bacteria. This means that each fly experimental group would have one species of bacteria in their microbiota, and therefore we can associate changes in fly behavior to the differences in the Drosophila microbiota. Having determined which bacteria affect the fly activity at low temperatures, we would use Metagenome-wide association to determine which bacterial genes are causing this change. Metagenome-wide association involves comparing the genomes of bacteria shown to cause changes in Drosophila activity in order to identify the similar protein-coding sequences that may be the causes of these changes in activity. To confirm the findings at that stage, the genes are expressed in a bacteria that doesn’t express them normally, and flies are analyzed to see if the changes in activity persist. If they do, we can safely say that the changes are due to that specific gene sequence. We expect that the mono-associated flies will show differences in activity level, because it has already been established in a previous study that the microbiota in the flies has a statistically significant effect on the activity level of the flies, meaning that the presence of certain bacterial cultures in the flies lead to a difference in activity. Therefore, if there is no difference in activity level, it is most likely due to the low temperatures, and it may then be necessary to perform the experiment again at different temperatures to determine at what point the temperature negates the effect caused by the bacteria present in the fly microbiota. We aim to study the relationship between the microbes present in the fly microbiota and their activity level at low temperatures and identify the mechanisms by which the microbes affect the fly activity level.

Authors: Lauryn Dupaix. Mentors: Riley Nelson. Insitution: Brigham Young University. Golger’s rule is an ecogeographic rule stating that animals in warm and humid climates are heavily pigmented, whereas animals in colder and drier climates are lightly pigmented. By using previously collected specimens of Proctacanthus sp. from across the U.S., we explore how this rule applies to several species of robber flies over time and geographic space. The average color of thorax was analyzed using a spectrophotometer which were used in correlation tests with climate measurements from where the specimen was collected. We found that this ecogeographic rule was not consistent in all four of the species of robber flies that were analyzed. Therefore, other studies of the applicability of ecogeographic rules are being assessed in this genus. This research helps define morphological variation in a widespread species and open avenues for more morphological analysis of insects in a changing climate.

Authors: Levi Pouwer, Keaton Helquist. Mentors: Jared Nielsen. Insitution: Brigham Young University. OBJECTIVE: To identify brain regions and networks underlying acquired-brain-injury-induced insomnia. BACKGROUND: In patients of tertiary-level mental healthcare facilities, symptoms of chronic insomnia are prevalent in 78.2% of the population. Additionally, among those who suffer from acquired brain injury, few symptoms are as generally pervasive as that of chronic insomnia. Meta-analyses of insomnia patients with fMRI data have failed to identify consistently affected brain regions. Individual studies have suggested several brain regions are involved in insomnia, including the anterior cingulum, orbitofrontal cortex, the insula, caudate nucleus, and the anterior capsula interna. However, few regions have consistently been implicated in the pathophysiology of insomnia. Moreover, little is known regarding the collective brain networks involving insomnia. DESIGN/METHODS: A comprehensive systematic literature review was conducted to identify case studies of individuals afflicted with acquired brain injury-induced insomnia (N=53). The review process was facilitated by employing Cadima, specialized literature review software. To establish a reference baseline for comparison, a substantial cohort of resting-state functional MRI (fMRI) scans from healthy control subjects (N=1000) was meticulously acquired. Subsequently, lesion network mapping analysis, as outlined by Fox et al. in 2018, was applied to the lesions observed in the 53 patients. This method allowed us to assess the impact on functional connectivity and analyze its implications by comparing the patients' lesion networks with the baseline scans obtained from healthy controls. RESULTS: In the context of lesion network mapping analysis, our study revealed that 45 out of 53 patients diagnosed with acquired brain injury-induced insomnia exhibited brain lesions that were functionally connected to both the left amygdala and the left anterior insula. Moreover, in 39 out of 57 patients, the lesion networks also included bilateral anterior insula and the hypothalamus. Comparative analysis of these lesions with a normative database unveiled a substantial disparity in functional connectivity to the left amygdala and left anterior insula in patients suffering from acquired brain injury-induced insomnia. This finding underscores the relevance of tissue within these regions and tissues functionally connected to these areas in relation to the onset of lesion-induced insomnia. CONCLUSION: The observed functional network connectivity among the left amygdala and the left insular region in relation to insomnia introduces a potentially more intricate mechanism underlying the onset of insomnia, in addition to the previously proposed factors such as hyperarousal in cortical regions and subcortical neuropathologies. Our findings highlight the possibility of a deeper connection between the initiation of insomnia and limbic structures, specifically emphasizing their role in emotion and sleep regulation. This suggests that the neural mechanisms influencing anxiety may have interrelated effects on the mechanisms influencing insomnia. It is evident that further investigation, particularly among psychiatric patients afflicted with insomnia, is warranted to delve into the activities occurring in these regions and to elucidate the potential interplay between anxiety and insomnia. This research paves the way for a deeper understanding of the complex relationship between neural pathways and the development of insomnia, ultimately offering valuable insights for future clinical and therapeutic considerations.

Authors: Brynlyn Hastings, Dante Cervantes, Jaime Analuisa. Mentors: Lindsay Chaney. Insitution: Snow College. Ants are a diverse and ecologically significant group of insects, yet their species identification in the field can be challenging due to their small size and morphological variation. In this study conducted at Snow College, we employed DNA barcoding techniques to unravel the ant species diversity within the local ecosystem of Snow College's Campus. Our research aimed to shed light on the ant species biodiversity concerning areas that had more human interference compared to those with little human interference contributing to a better understanding of local biodiversity.

Authors: Joshua Thlaman, Brynlei Luke, Emily Pattee. Mentors: Lindsay Chaney. Insitution: Snow College. We tested if grasshopper species varied based on locations with constant human interaction vs. locations with little to no human interaction within Sanpete county. Due to how close, (a 6 mile radius), the collection locations are, it was logically reasoned that there will be no differences in grasshopper species between Ephraim and Manti Utah.​This study was performed with grasshoppers collected in Sanpete County: specifically, the town of Ephraim, Ephraim canyon, the town of Manti, and Manti canyon. To do this, we collected grasshoppers from each of the mentioned locations, extracted the DNA samples by humanely freezing the grasshoppers and collected small amounts of the grasshoppers, amplified the DNA samples using PCR, and sequenced the PCR products to help identify the grasshopper species collected using BLAST. ​Our experiment results showed that there was a large variation of species collected, as well as many similar species were present in all collection locations. Ephraim housing and Ephraim canyon only had one type of species present (Melanoplus sanguinipes), while Manti housing showed three other species (Melanoplus differentialis, Dissosteira carolina, Trimerotropis pallidipennis), and Manti canyon showed another species type (Trimerotropis cincta). This suggests that there is more variation within Manti housing and Manti canyon, no variation within Ephraim housing and Ephraim canyon, and only some variation between Manti and Ephraim.

Authors: Michal Hansen, Nathan Werner. Mentors: Nathan Werner. Insitution: Southern Utah University. The preparation of pharmaceuticals and fine chemicals requires chemical reactions that make carbon-carbon bonds. Carbon atoms of three different hybridazations are known: sp3, sp2 and sp. Most reactions are specific to the hybridization of the carbon atom. We will study a reaction to bond an sp2-hybridized carbon to an sp3-hybridized carbon. This reaction could then be used to make interesting molecules that contain similar bonds. Specifically, the synthesis of 1,3-diphenylpropene will begin with a hydroboration reaction of phenylacetylene with pinacolborane. These two starting materials are combined with a 9-BBN-catalyst and heated to reflux in tetrahydrofuran at 65 degrees Celsius for 1 hour. The hydroboration product is then purified by aqueous extraction and silica gel flash chromatography. The product from the hydroboration reaction contains an sp2-hydridized carbon-boron bond and is then combined with benzyl bromide that contains a sp3-carbon-bromine bond. This is the reaction that we are studying. They are heated in DMF solvent with a palladium catalyst, ligand and base at 90 degrees Celsius for 1.5 hours. Once the reaction is complete, the cross-coupling product is purified by aqueous extraction and silica gel flash chromatography.

Authors: Cate Bateman, Jose Aurelio De La Rocha Tapia, Ethan Fulwood, Arianna Harrington. Mentors: Arianna Harrington. Insitution: Southern Utah University. The Eocene-Oligocene boundary in North America was marked by climatic cooling and drying. This transition is a key time in mammalian evolution, and studying fossils spanning this boundary could yield important information about how fauna responded to the climatic change. McCarty’s Mountain, a fossil rich geological feature located in Madison County, Montana, has long intrigued scientists due to the many insights it holds into the terrestrial fauna of the region. The sites from McCarty’s Mountain date from the Chadronian North American Land Mammal Age, which corresponds to the late Eocene epoch. The geological strata to produce these fossils were thought to have been deposited in a floodplain, yielding remarkably preserved fossils which have been collected and studied since the early 1900’s. However, the microfossils from this locality have been understudied despite the insights it could provide to the terrestrial Eocene-Oligocene transition in North America. In this study, we aim to close this gap by analyzing microfossils picked from matrix collected from McCarty’s Mountain localities in summer 2023. Preliminarily, we have processed about ⅛ of the collected sediments, and have found several identifiable elements including mammalian teeth, vertebrae, and long bones. We will continue this work by washing and soaking the remaining matrix and quantifying the microfossils retrieved. The fossils recovered may provide future insight to changes in microfauna across the Eocene-Oligocene boundary.

Authors: Ashley Hartwig, Dr. Weeg. Mentors: Matthew Weeg. Insitution: Southern Utah University. The physiological benefits of petting pet cats, dogs, and horses have been thoroughly investigated for decades. Studies suggest that the act of handling and being around pets can offer direct cardiovascular benefits, such as lower blood pressure and heart rate as well as indirect benefits such as lowering triglyceride and cholesterol levels. However, the ability of pet reptiles to offer similar benefits has not been explored. In this work, we investigated the effects of reptile handling on the handler, expressed by cardiovascular variables. An automated blood pressure device was used to obtain the blood pressure and heart rate of participants every five minutes over a 20-minute time period. Three different conditions were tested for each participant: one where they handled a snake (Python regius), one where they handled a lizard (Pogona vitticeps), and one where they handled a stuffed animal , which served as the control. Overall, we saw a significant decrease in the systolic blood pressure in subjects during both reptile conditions compared to the control , though there was no change in diastolic blood pressure, mean arterial pressure (MAP), or heart rate. A decrease in systolic blood pressure has also been documented in studies involving mammalian companion animals. Therefore, our results provide evidence that handling snakes and lizards might provide some of the same cardiovascular benefits as handling cats, dogs, or horses. If so, this lends support to the argument that lizards and snakes can also be considered companion animals. These conclusions can be used to advocate for proper reptile husbandry and conservation efforts to protect native species in the fight against stigmas surrounding reptiles.

Authors: Clista Galecki. Mentors: William Anderegg. Insitution: University of Utah. This project will determine whether exposure to wildfire impacts a plant's ability to acclimate to summer drought. This is important because with rising temperatures and droughts there is an increase of wildfires (Diffenbaugh et al., 2015). This question will be address by comparing drought physiological traits of a native tree species, Utah juniper, in burned versus unburned sites in central Utah. The goal of the project is to determine whether plants that survive wildfires are more or less drought resilient. This research may provide insight into whether various tree species are better adapted to survive droughts and wildfires which will help in future ecology predictions of the future landscapes.There are multiple factors in why forests respond differently to drought. The factors include elevation, prior exposure to fire, prior exposure to drought, clade, and species (Anderegg et al., 2020). Severe drought or heat from a fire can result in hydraulic failure (McDowell et al., 2008). This means that the plant has loses its ability to sufficiently move water from the roots to the leaves. Which could indicate that trees burned in wildfires will not be better adapted to summer drought. Whereas there is a possibility that if the tree has had sufficient amount of time to recover from a stressing event that it could be better adapted for future droughts (Anderegg et al, 2020). My project will focus specifically on the effects of prior exposure to fire on Utah juniper (Juniperus osteosperma) drought acclimation ability.

Authors: Logan Whitney, Jaren Wilson, Elley Colledge, Jamie Thygerson. Mentors: Jessica Pullan. Insitution: Southern Utah University. Exosomes are nanosized (40-150 nm) extracellular vesicles (EVs) secreted from cells that play a vital role in the human body's intracellular communication.1 The use of bovine-derived exosomes as a drug delivery vehicle provides an innovative approach to targeted drug delivery4 due to their stability, nontoxicity, non-immunogenic properties, and cost efficiency.5,6,7,8,9 The purpose of our research is to establish and refine a raw bovine milk exosome isolation protocol to determine the quality of exosomes. Current standards for small EV identification are currently being established and regulated by the International Society of Extracellular Vesicles (ISEV) but center around three main categories: proteins, nucleic acids, and lipids.2 To refer to EVs as exosomes, they must be of endosome origin, otherwise they are referred to as ectosomes, and must be appropriately referred to by their size.13 Upon isolation, we verified the morphology of the small EV’s using Scanning Electron Microscopy, allowing visualization of the size and shape of the small EVs. Our research methods for the quantification of surface proteins include Rose Bengal Assay12 and Bradford Assay. Examination using exosome-specific cell surface markers, such as CD63, demonstrates protein functionality of the small EVs. Quantification of lipids allows for comparison of lipid concentration to surface protein concentration for further evidence of exosome identification. Lastly, RNA quantification ensured the small EVs met the concentration threshold to be defined as exosomes. The combination of these methods will further the work in bovine milk extracellular vesicles by yielding a low-cost, high-yield protocol for the isolation and characterization of EVs that can be further used as biomarkers, drug carriers, and a variety of platforms of delivery to physiological targets. [1] Rayamajhi, S., Nguyen T., Marasini R., Aryal S. Macrophage-derived exosome-mimetic hybrid [2] Théry, C., Witwer, K.W., Aikawa, E., Alcaraz, M.J., Anderson, J.D., Andriantsitohaina, R., et al. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines.  Journal of Extracellular Vesicles 2018; 7:1. [3] Pullan, J. E., Confeld, M. I., Osborn, J. K., Kim, J., Sarkar, K., and Mallik, S. Exosomes as drug carriers for cancer therapy. Molecular Pharmaceutics 2019; 16:1789–1798. vesicles for tumor targeted drug delivery. Acta Biomaterialia 2019; 94:482-494.  [4] He J, Ren W, Wang W, et al. Exosomal targeting and its potential clinical application. Drug Deliv Transl Res 2022;12(10):2385-2402. [5] Munagala R, Aqil F, Jeyabalan J, Gupta RC. Bovine milk-derived exosomes for drug delivery. Cancer Lett. 2016; 371(1):48-61. [6] Rashidi M, Bijari S, Khazaei AH, Shojaei-Ghahrizjani F, Rezakhani L. The role of milk-derived exosomes in the treatment of diseases. Front Genet 2022;13:1009338.  [7]García-Martínez J, Pérez-Castillo ÍM, Salto R, López-Pedrosa JM, Rueda R, Girón MD. Beneficial Effects of Bovine Milk Exosomes in Metabolic Interorgan Cross-Talk. Nutrients 2022;14(7):1442.  [8] Mashouri, L., Yousefi, H., Aref, A.R. et al. Exosomes: composition, biogenesis, and mechanisms in cancer metastasis and drug resistance. Mol Cancer 2019; 18:75.  [9] Radha Munagala, Hina Kausar, Charu Munjal, Ramesh C. Gupta, Withaferin A induces p53-dependent apoptosis by repression of HPV oncogenes and upregulation of tumor suppressor proteins in human cervical cancer cells, Carcinogenesis 2011; 11:1697–1705. [10] Wijenayake S., Eisha S., Tawhidi Z., Pitino M.A., Steele M.A., Fleming A.S., et al. Comparison of methods for pre-processing, exosome isolation, and RNA extraction in unpasteurized bovine and human milk. PLoS ONE 2021;16:e0257633. [11] Osteikoetxea X., Balogh A., Szabó-Taylor K., Németh A., Szabó T.G., Pálóczi K., et al. Improved Characterization of EV Preparations Based on Protein to Lipid Ratio and Lipid Properties. PLOS ONE 2015;10:e0121184. [12] Kishore, S.; Maruthamuthu, M. Binding of Rose Bengal onto Bovine Serum Albumin. Proceedings / Indian Academy of Sciences 1993, 105 (4–5), 279–285. DOI:10.1007/bf02866917. [13] Théry, C., Witwer, K. W., Aikawa, E. et al. (2018). Minimal information for studies of extracellular vesicles 2018 (MISEV2018): A position statement of the International Society for extracellular vesicles and update of the MISEV2014 guidelines. Journal of Extracellular Vesicles, 7(1). https://doi.org/10.1080/20013078.2018.1535750z

Authors: Mackay B Steffensen, AustinRyan A Kirkby, Kieran L Richards. Mentors: Mackay B Steffensen. Insitution: Southern Utah University. Cyanuric chloride, a 1,3,5-trichlorotriazine, serves as a valuable platform for constructing innovative molecules. This project explores the unique reactivity of triazines to synthesize triazine crown ethers—a class of cyclic polyethers with a central cavity capable of selectively binding metal ions and polar molecules. Temperature-controlled nucleophilic aromatic substitution is employed to selectively substitute the triazine ring, providing precise control over the reaction. The first substitution with a primary amine nucleophile occurs rapidly at 0 °C, while subsequent substitutions at room temperature or 70 °C necessitate longer reaction times. This controlled approach enables the incorporation of three different amines.The project aims to synthesize triazine crown ethers through temperature-dependent substitution using two different methods. The first approach employs a diamine with an ether linkage in the presence of a base, facilitating consecutive substitutions to produce the target molecule. Intramolecular reactions are favored, but intermolecular reactions may lead to unexpected yet valuable products. The second method utilizes templated synthesis, taking advantage of the affinity of crown ethers for metal cations to promote cyclization reactions. A traditional route involving post-synthetic cyclization with ethylene glycol is also considered.The successful synthesis of these molecules opens up opportunities for further research, including characterizing their affinity for metals and exploring other unique properties. The acquired knowledge can be applied to construct more complex molecular structures, making this work valuable in materials science, analytical chemistry, and pharmaceuticals.

Authors: Sydney Pruett. Mentors: Roger Gold. Insitution: Southern Utah University. Microbiologically Induced Calcite Precipitation (MICP) presents an innovative avenue for sustainable construction and environmental remediation. This biogenic process leverages the enzymatic hydrolysis of urea by microorganisms to generate calcium carbonate crystals, offering a green alternative with carbon sequestration potential, enhanced structural durability, and self-healing capabilities. Sporosarcina pasteurii is currently the predominant microorganism employed in biobrick production due to its notable urease activity. This study endeavors to assess the MICP potential of less explored bacterial strains with comparable urease activity levels. The strains under investigation include Sporosarcina ureae, Bacillus sphaericus, Bacillus thuringiensis, Bacillus cereus and Bacillus stearothermophilus. Additionally, we aim to shed light on the viability of these alternative microorganisms for MICP applications, contributing to a broader understanding of this promising technology's adaptability and potential implications for sustainable construction practices.

Authors: Ann Day, Shawn Coon. Mentors: Shawn Coon. Insitution: Westminster College. The decades long movement to discredit, defund, and de-professionalize public education has taken many forms. Since the COVID-19 pandemic, policymakers have increasingly attempted to undermine public education through state specific programs (Bacher-Hicks et tal., 2023; Peele & Riser-Kositsky, 2020; Friedman & Young, 2022; Perera, 2023; Vasallo, 2023; Walker, 2022). These policies have taken many forms and attempted to address multiple perceived problems. However, the one commonality between all of these policies is that they perpetuate a trend of educational policymaking that tacitly or actively attempts to de-professionalize teachers and teaching (Apple, 2004; Milner, 2013). The state of Utah, and it’s 2023 legislative session, offers a compelling case study into why this legislation continues to emerge, the rationale behind this movement, and the impact these policies have on educators.

Authors: Isaac Wilson, Bree Wilson. Mentors: Arianna Harrington. Insitution: Southern Utah University. Understanding the body size of an organism is crucial for discussing its ecological role, potential trophic impact on its environment, and life history. The sauropod dinosaurs represent the largest land animals known, reaching masses many times that of modern elephants and rivaling the most massive cetaceans in some cases. This study attempts to quantify the body size variation between average and maximum sauropod dinosaurs by using Camarasaurus as a focal genus and comparing appendicular elements from the same quarries. Intraquarry rather than intraspecific variation is chosen due to the difficulty of diagnosing limb elements to the species level, and limb elements are known to scale isometrically in Camarasaurus. In addition to Camarasaurus-centric measurements, extant and extinct ecological analogues with recorded body size ranges are also used to estimate body size variation. While their life histories are not well-known, histological analysis indicates that sauropods were likely tachymetabolic endotherms, and their growth curves were most similar to birds and large herbivorous mammals in terms of extant taxa. This is in contrast to basal sauropodomorphs like Plateosaurus, which is demonstrated to exhibit high developmental plasticity. Minimum intraspecific size variation, collected from the literature and based on birds, nonavian dinosaurs, and large herbivorous mammals suggest that the largest representatives of any given sauropod species could have been between 139% and well over 200% larger than the average. Applying preliminary ratios from the data collected so far to the largest well-preserved sauropod, Argentinosaurus huinculensis, results in a plausible maximal mass of 137-177 tonnes for the genus.

Authors: Seunghwan Shin, Sydney Coates. Mentors: Guizella Rocabado. Insitution: Southern Utah University. Organic chemistry is one of the most feared and failed courses in the undergraduate curriculum. Consequently, studying what makes this course “too difficult” as perceived by students is worthwhile because these perceptions result in many students not considering STEM majors because they require chemistry courses. Our research group has investigated perceived costs in general chemistry, and this study expands our understanding of these constructs in organic chemistry. Students’ perceived costs of a chemistry class can be many, such as task effort, loss of valued alternatives, emotional, and others. These costs might be overcome by students’ interests and goals, yet the level of perceived costs might have a lasting impact on the students’ overall perception of chemistry and their desire to pursue chemistry and other STEM careers in the future. In this study we investigated the mentioned subclasses of perceived costs, other salient perceived costs, and mastery or performance goal orientations and the impact these constructs may have on students’ experiences in organic chemistry classrooms. Expanding on a previous study in general chemistry, we present the results of interviews we conducted with students in this class. Our results reveal that students struggle with the content, pace, and overall difficulty of the material. These results are not surprising; however, we also uncovered the students’ deep desire to learn and understand the material, the motivating effect of having a good relationship with their professor, and many other themes that emerged from our conversations.

Authors: Gabriella Proctor, Luke Alder. Mentors: Marcos Corchando Sonera. Insitution: Southern Utah University. The chytrid fungus Batrachochytrium dendrobatidis (Bd) is known to cause the disease chytridiomycosis and is notorious for its role in decimating anuran populations worldwide. A highly prevalent invasive species, the American bullfrog (Lithobates catesbeianus), is understood to play an important role in the transmission of this disease in invaded regions. Notably, American bullfrogs are often largely immune to the effects of Bd, making them important reservoirs of the pathogen. Within southern Utah, this species has successfully invaded the Virgin River watershed, yet this population has not been the subject of prior study regarding its potential as a reservoir for Bd. We hypothesize that the Utah population has also been infected with Bd, potentially endangering native amphibian species. We sampled 46 bullfrog specimens from eight distinct aquatic habitats within the Virgin River basin in southwest Utah. Skin swabs will undergo purification and quantification, followed by PCR and gel electrophoresis to visualize and confirm results. We hope that our findings will be valuable in comprehending the impact of invasive American bullfrogs on amphibians within the Colorado River basin, particularly in relation to the spread and effects of chytridiomycosis.

Authors: Braden Nelson. Mentors: Nathan S Werner. Insitution: Southern Utah University. The stereocontrolled palladium-catalyzed Suzuki-Miyaura cross-coupling reaction of (E)-2-phenylethenylboronic acid pinacol ester with aryl chlorides to produce (E)-stilbene derivatives was studied. (E)-2-Phenylethenylboronic acid pinacol ester was synthesized by 9-borobicyclo[3.3.1]nonane (9-BBN) catalyzed hydroboration reaction of phenylacetylene with pinacolborane. The reaction conditions of the palladium-catalyzed cross-coupling reaction of this reagent with aryl chlorides were then evaluated to maximize the yield of the cross-coupled product. The maximum yield of (E)-stilbene, 74%, was obtained when the amount of (E)-2-phenylethenylboronic acid pinacol ester was increased from 1.2 to 1.5 equivalents, RuPhos was used as the ligand, K2CO3 was used as the base, and DMF was used as the reaction solvent at 90 °C. Four other aryl chlorides bearing electron-withdrawing and electron-donating substituents in the ortho- and para-positions were found to undergo facile coupling in 91-100% isolated yield.

Authors: Derek Baker, Lauren Jensen, Dagney Goodfellow, Seunghwan Shin. Mentors: Guizella Rocabado. Insitution: Southern Utah University. Differences in sexes have long been speculated, yet their application in chemistry learning environments has not been well researched. Literature shows that males have larger cerebral volumes for visual capacities more often associated with hands-on projects and courses than female brains. And female brains have larger cerebral volumes for areas associated with memorization and socialization which more highly correlate with lecture-based learning. Given the high attrition of women in chemistry courses, we investigated this phenomenon in organic chemistry topics that are highly visual (e.g. chirality, Newman projections, chair conformations, bond rotations, etc.) and topics that require more memorization (e.g., functional groups, energy values associated with sterics, nomenclature, definitions, etc.). We conducted an anonymous survey with students who are currently enrolled in organic chemistry or have taken organic chemistry in the past. The questions varied in difficulty as well as levels of visualization or memorization required. The results show correlation with the literature that males score better in the visual-based questions, and females score better in the memorization-based questions. In addition, we conducted interviews with male and female students in organic chemistry, and found that the female students self-generated various techniques to visualize 3D rotations (e.g., use their hands, draw arrows, etc). While most male students didn’t adopt these techniques because they were able to do the 3D rotations in their head. With these results, we plan to design course interventions to bridge the gaps between the extremes of visualization vs. memorization-based concepts for the students.