Fine Arts

Authors: Zach Fiore. Mentors: Jared Nielsen. Insitution: Brigham Young University. Gait apraxia is a type of apraxia that affects lower limb use in walking. It is characterized by difficulty initiating gait, freezing of gait, and other gait disturbances that cannot be attributed to complications affecting sensory, motor, or cerebellar function, psychiatric disease, nor ataxia. Symptoms often present following brain trauma. Previous research has indicated that gait apraxia may be linked to lesions in the frontal lobes, basal ganglia and supplementary motor area. However, the specific cerebral location has been debated with minimal research done on the symptom’s implicated neural circuits. The purpose of this study is to determine the networks in the brain that are involved in the pathophysiology of gait apraxia. To determine this, we used the lesion network mapping method. A systematic literature review was performed, with specific inclusion criteria, to find case studies of patients presenting with gait apraxia stemming from acquired brain injury (n=15). Lesion network mapping analysis (Fox et al., 2018) was performed on 15 cases with a large cohort of healthy control resting-state scans (n=1000). The analysis showed that lesions exhibited functional connectivity to the bilateral medial dorsal and pulvinar nuclei of the thalami (n=15), which supports previous associations of basal ganglia damage contributing to gait apraxia. A novel region, the cingulate cortex (n=15), was also found to be functionally connected to the lesion networks. This region is a part of the cingulo-opercular network, responsible for many functions, including action. This network has recently been found to display strong functional connectivity with the somato-cognitive action network, responsible for coordinating movements with cognitive processes. Further research is necessary to determine the mechanism of how these networks interact in contributing to gait apraxia.

Authors: Joshua Hutchins, Kevin Wong. Mentors: Dario Mizrachi. Insitution: Brigham Young University. Tight junctions (TJ’s) are composed of mainly three types of cell-adhesion molecules (CAMs) that regulate paracellular permeability in epithelial and endothelial cells. These are claudins (CLDNs), occludin (OCLN), and junctional adhesion molecules (JAMs).There are, however, several (27) isoforms of the claudin molecule, all of which are suspected to have different strengths and other properties in cell adhesion. Currently, the comparative strength of the interactions between different CAMs are unknown and no easily replicable model of a TJ has been created. To address this question we resourced to bacterial expression of these mammalian proteins. MG1655 E. coli cells (with flagellum) were transformed to express claudins 1, 2, 3, 5, and 10 as well as occludin and plated on 0.2% agar plates, allowing them to swim overnight. This allowed for a qualitative spectrum of strengths of the CAMs based on how far the cells were able to spread throughout the plate. Cells that interacted strongly swam less. This technique was applied to the case of the Atlantic Salmon. It swims both in fresh and ocean water. As it transitions, the TJs in its skin changes its composition of claudins. We were able to determine that the set of claudins employed during ocean water swimming are capable of stronger strength. This is consistent with the changes in osmolarity dictated by the amount of solute in the ocean water.

Authors: Jacob Armstrong, Tyler Standifird. Mentors: Tyler Standifird. Insitution: Utah Valley University. Lower Limb Asymmetries in Unilateral Jump Mechanics as Possible Risk Factor for Injury in Collegiate WrestlersJacob Armstrong and Tyler Standifird 1Utah Valley University, Orem, Ut. Injuries impose a multi-faceted burden on athletes, coaches, and sporting organizations, encompassing physical, financial, and psychological ramifications. Determining risk factors and identifying them in athletes has been a widespread goal amongst researchers and practitioners in attempts to reduce injuries. Investigating Limb asymmetries, or limb imbalances, has been a common approach in identifying such factors but has shown inconsistent correlations with injuries (Melony, 2018). Moreover, much of the current research investigates asymmetries of outward limb performance (i.e., Strength, flexibility, jump performance etc.) and not asymmetries of internal joint mechanics. Identifying relationships between internal joint asymmetries and lower limb injuries can further aid coaches, practitioners and sporting organizations in effectively identifying injury risks. Purpose: The purpose of this study was to investigate internal joint asymmetries of the lower limbs during unilateral jump testing and their association with injuries in collegiate wrestlers. Methods: 23 healthy Division 1 NCAA collegiate wrestlers performed single leg depth jumps from a 12-inch platform unto force plates (Bertec Inc 1000 hz). Additionally, markerless motion capture technology was used to collect video data using Qualisys Mocap cameras (Qualisys North America, Inc. Buffalo Grove, Ill). Imagery was then processed in Theia (Theia Markerless, Inc. Kingston, Ontario) to produce 3d joint locations for all the joints of the lower extremity. Each wrestler performed 3 jumps with the left leg and then 3 with the right leg for a total of 6 jump attempts. 7 total variables were calculated and recorded in Visual 3D software (Dolomite Enterprises, LLC. Tallahassee, FL) for each wrestler and leg: drop max vertical force, vertical loading rate on drop, peak knee adduction angle, peak knee abduction moment, peak knee adduction moment, peak knee flexion angle, peak knee extension moment. Averages, standard deviations and percent differences were also calculated and compared between limbs. Injury history data was also collected from each participant from the year 2021 - present day.Results: Averaging found asymmetries in peak knee adduction angle with the left knee being greater than the right by 4.9 degrees (p=0.001), peak knee abduction moment with the right knee being greater than the left by .95 newton meters (p=0.001), and peak knee adduction moment with the left knee being greater than the right by .44 newton meters (p=0.001). All significant asymmetries were found in the frontal plane. No significant asymmetries were found in the sagittal plane. Injury history report found a total of 24 seperate lower limb injuries within the described timeframe. 16 associated with the right limb and 8 associated with the left. A total of 5 ACL injuries, 8 MCL injuries, 5 LCL Injuries, 3 meniscus injuries, and 3 ankle injuries. Conclusion: Asymmetry trends in collegiate wrestlers potentially indicate that internal joint asymmetry is specific to sport or activity. Also, internal joint asymmetry could be a potential indicator for training protocols for overall better movement and health within the studied population. Asymmetrical values of the peak knee adduction angle, peak knee abduction moment, and peak knee adduction moment combined with the frequency of knee injuries of the participants indicate possible positive correlations between the magnitude of asymmetry discovered and injury risk.

Authors: Gabe Matthews, Easton Eddie. Mentors: Jennifer Meyer. Insitution: Utah Tech University. Diabetes is a prevalent chronic health condition associated with significant complications, including diabetic kidney disease. The accumulation of elevated glucose levels in cells triggers an upregulation of NADPH Oxidase (NOX) expression, contributing to diabetic kidney disease. NOX activation results in an increased production of reactive oxygen species (ROS), inducing oxidative stress and cellular proliferation. Pterostilbene, recognized for its natural antioxidant properties, has demonstrated efficacy in reducing oxidative stress across various cell types. This study focuses on elucidating the NOX pathways in endothelial cells exposed to hyperglycemic conditions and assessing the extent of oxidative stress reduction with the introduction of pterostilbene. To quantify cellular oxidative stress, we will employ an Amplex Red assay to measure superoxide and hydrogen peroxide levels within the cells. Quantitative polymerase chain reaction (qPCR) will also be utilized to assess NOX protein gene expression at the mRNA level. To complement these methods, a western blot analysis is conducted to quantify NOX protein concentrations under distinct environmental conditions. Our research aims to shed light on the potential of pterostilbene as a therapeutic agent in mitigating oxidative stress associated with hyperglycemic conditions in endothelial cells.

Authors: Lillian Davis, Paul B Frandsen. Mentors: Paul B. Frandsen. Insitution: Brigham Young University. Insects of the order Embioptera (webspinners) typically live in colonies that can cover the ground of entire fields with their silk-constructed galleries, providing them with camouflage from predators and cover from the rain. Uniquely amongst silk-producing insects, webspinners possess silk glands in their forelimbs and, like spiders, retain the ability to produce silk throughout their life history. They typically live together in semi-social colonies, feeding off of decaying plant matter. Previous studies have been limited to morphological descriptions of species, testing various qualities of their silk, and a few molecular studies based on short DNA sequences. Here we present the genome of Apostonia ceylonica, a webspinner species from India, providing the first embiopteran genome assembly, opening the door to a multitude of analyses. At a haploid size of approximately 3.1 gigabases, their genome is significantly larger than previous estimates, containing a significant number of repeating segments. This data provides valuable information for future studies into the genes that control silk production, information for identifying this species through meta-barcoding, and data that could improve phylogenetic trees. By evaluating the effect of repetitive elements on their oversized genomes, we gain insights into the evolutionary history of Apostonia ceylonica.

Authors: Hazel Ticas, Daniel Scott. Mentors: Daniel Scott. Insitution: Utah Valley University. The COVID-19 pandemic’s results, though highly publicized throughout its early years, fell out of the media’s attention over time. Despite the lower volume of media coverage, the effects of the pandemic remain ongoing. In an effort to maintain accurate information about the most current impacts of COVID-19, this study will review relative COVID-19 case amounts compared to population density for four populations during the omicron peak between the years of 2021 and 2022.. The four populations are: counties in New York, United States; counties in California, United States; a collection of the largest counties from the other 48 United States, and the states in Sweden. The population density for each of the four populations was corrected using Google Maps in order to eliminate areas that didn’t have any residents, but were part of the county or state boundaries. Boundaries have been defined as anything thats stops the spread of COVID-19, said boundary can be geographical or self-isolation. We aim to analyze the data trends of how Omicron spreads in relation to population density and boundary effects by comparing the ratio of virus spreadability to boundaries on each population. The goal is to create a model that can be applied to COVID-19.

Authors: Tiba Hasan, Elise Larsen, McKell Gillins. Mentors: Jodie Lopez. Insitution: Utah Valley University. AbstractThis paper discusses studies that have researched the most effective forms of teeth whitening and the mechanism behind it. Studies discuss the oxidation of double bonds which in turn lighten the chromogens impacting the overall coloration of dentition. The three main forms of treatment that were considered in these studies include in-office treatment, at home treatment and OTC products. At home overnight bleaching proved to have the best results for both immediate and post 10 week results. At-home treatment under dentist’s care involves lower concentration of hydrogen peroxide or carbamide peroxide ranging from three to ten percent thus it takes longer time than in-office treatment (Leh, B. 2018). Factors such as food intake, beverages, smoking, and oral hygiene care can all impact the efficiency of bleaching methods. While some toothpaste brands, like charcoal toothpaste, claim to whiten teeth, research suggests that these abrasive toothpastes may lead to enamel wear. Furthermore, studies have compared the effects of chemical and herbal toothpaste and found that both can produce similar whitening effects. However, chemical products tend to be more harmful compared to herbal alternatives As dental esthetics have become increasingly important to western populations, more whitening products are readily available for consumer use. Studies warn about the adverse risks and effects of dental bleaching to be aware of for both the consumer and those applying or prescribing bleaching methods, therefore it is important for hygienists and others in the dental field to become educated and aware of the increasing bleaching product selection.

Authors: Michaela Lovejoy, Rylee Udom, Lindsey Fry, Hailey Roubidoux, Makayla Dunbar, Kali Hunt, Mandy Gibbs, Lisa Welch. Mentors: Lisa Welch. Insitution: Utah Tech University. Traditional dental radiography education necessitates the exposure of both clinician and patients to radiation to build clinician skill. Virtual reality (VR) may enable practitioners to learn valuable hands on skills without the use of expensive equipment and without patient or operator exposure to radiation. The purpose of the research is to determine if access to practice in virtual reality (VR) using a specifically designed application, builds skill competency in dental radiology compared with traditional practice methods. A convenience sample of 24 first year dental hygiene students at Utah Tech University were recruited and randomly assigned to the test and control groups. Test group participants were given access to a VR headset with a dental radiology skill practice application installed. Control group participants were given access to traditional dental radiography skill practice methods. To encourage participation, an incentive of a $10 Amazon gift card upon completion of the study was offered. Data will be collected via skill competency evaluation at two-months, mid-term grade scores and final grade scores from the participants’ dental radiology laboratory course. To increase internal validity and reliability, investigators responsible for skill competency evaluation will be blinded and undergo both intra and inter-rater reliability testing to 95% agreement. Upon completion of the Fall semester 2023, data will be analyzed using ANOVA repeated measures between factors accounting for the two independent variables of traditional practice and practice in VR and the dependent variables of competency assessment scores, mid-term and final clinical course grades. Alpha will be set at 0.05. Should it be determined that practice in VR contributes to student learning, VR may enable students to practice additional health science skills in a safe, low stakes environment; thus, increasing student experiential learning and contributing to increased student and patient safety.

Authors: Britt Wyatt, Alyssa Habel, Jess Cusick, Heath Ogden, Josh Premo. Mentors: Dr.Britt Wyatt. Insitution: Utah Valley University. Vaccines are crucial to maintaining healthy communities as they provide immunological protection against a variety of infections (Geoghegan, S. et al. 2020). By providing immunity, vaccines prevent serious illnesses, reduce disease severity, and protect communities. However, in 2019 the World Health Organization (WHO) declared vaccine hesitancy (resistance to getting vaccinated) as one of the top 10 dangers to global health (Dubé, Ève, et al. 2021). Previous studies have shown that certain communities are more vaccine hesitant than others. For example, individuals who are religious, conservative, Black, and women have been found to be more vaccine hesitant than other individuals (Savoia, E. et al., 2021) (Gowda C. & Dempsey, A., 2013). Therefore, we wanted to investigate vaccine hesitancy at an open enrollment institution that serves a unique student population where ~70% of students are members of The Church of Jesus Christ of Latter-day Saints and ~30% are non-traditional (over the age of 24) (UVU IR, 2023). Compared to the overall 1.6% of individuals in the United States who are members of The Church of Jesus Christ of Latter-day Saints (Pew Research Center, 2023), the identities of the students attending the open enrollment institution are vastly underrepresented in education research that has been conducted at other institutions. To investigate vaccine hesitancy, a survey was given to 1,444 students in the beginning of the Fall 2023 semester asking questions around vaccine safety and support. Preliminary results indicate that 70% of non-religious students will choose to be vaccinated against known and new diseases compared to 37% of religious students. 53% of non-religious students think that vaccine mandates are appropriate compared to 25% of religious students. These results indicate more vaccine hesitancy with religious students compared to non-religious students. As religious students make up most of the student population, results can help inform instructors and/or other stakeholders on scientific literacy topics to support in the academic setting. Lastly, further investigation on the intersectionality of identities will be explored such as gender and religion as women make roughly 80% of health-related decisions in their family unit. If religious women are more likely to be vaccine hesitant, development of strategies to support those students in making evidence-based decisions could be a future aim.

Authors: Coleman Judd, Alexander Beagley. Mentors: Michelle Culumber. Insitution: Weber State University. Due to the overuse of antibiotics in industrial, agricultural, and medical settings, antibiotic-resistant bacteria are becoming more prevalent in various unique settings. We set out with the goal of identifying antibiotic-resistant bacteria from the Great Salt Lake waterfront. After isolating colonies on agar containing the antibiotic Tetracycline, we performed 16S rRNA gene sequencing and identified three separate Enterobacteriaceae species all exhibiting varying levels of antibiotic resistance. We performed an array of biochemical and physiological tests to further distinguish the Enterobacteriaceae species. Full spectrum biochemical tests indicate two near identical species followed by a third, novel species as both a phenotypic and biochemical outlier. Antibiotic resistant bacteria within the Great Salt Lake region present an ever-growing dilemma of clinical and environmental danger that accompanies antibiotic resistant microbes.

Authors: Anthony Cornwall, Jake Rhea, Travis Ficklin. Mentors: Travis Ficklin. Insitution: Utah Tech University. The purpose of this study was to examine the correlation among various barbell tracking methods for both validity and between-method variability. Back squat and snatch lifts were simultaneously tracked using three systems: a corded linear transducer system (Tendo), a laser-based system (Flex), and a reflective marker multi-camera motion capture system (3D). The 3D system was considered a gold standard for validation. Nine repetitions each of barbell back squat and a snatch lifts were executed. Each system simultaneously tracked peak velocity, peak power, mean velocity, mean power, and displacement of the barbell for both lifts. All correlations were tested using Pearson Product Moment, with alpha set a priori at 0.05. Correlations among tracking methods were high for the back squat, and considerably lower for the snatch lift. Correlations for displacement were poor among all methods (-0.63 < r < 0.65). Correlations for velocities and powers were strong in the back squat (0.79 < r < 0.99), but weak-to-moderate for the snatch (-0.31 < r < 0.63). A discussion will be presented of tracking methods and lift mechanics, which can explain some, but not all, disparities among methods.

Authors: Dylan Snook, Anthony Cornwall, Travis Ficklin. Mentors: Travis Ficklin. Insitution: Utah Tech University. The purposes of this study were to (a) experimentally measure the moment of inertia of a regulation baseball and (b) use the same method to measure the new moment of inertia of a modified baseball about orthogonal axes. The moment of inertia (I) of a rolling sphere follows the form I = k*m*r2, where m = ball mass, r = ball radius, and k represents a constant specific to the ball. Because baseballs are multi-layered of varying densities, they were not treated as a solid spheres. The balls were rolled multiple times down a plane inclined at 4.55 degrees and acceleration was derived by video tracking the ball using a camera with optical axis perpendicular to the plane of the ball’s motion. Position and velocity were derived during the rolling acceleration, and the slope of velocity was taken to be the rolling acceleration (acc). This was then used in the equation: k = (g sin(theta)/acc) – 1, where g is the acceleration due to gravity and theta is the angle of inclination of the plane. The variable k was calculated based upon average acceleration over many rolling trials and then used to finally calculate I for each ball. Results for the regulation ball and for the modified balls will be presented for multiple axes.

Authors: Lauryn Dupaix. Mentors: Riley Nelson. Insitution: Brigham Young University. Golger’s rule is an ecogeographic rule stating that animals in warm and humid climates are heavily pigmented, whereas animals in colder and drier climates are lightly pigmented. By using previously collected specimens of Proctacanthus sp. from across the U.S., we explore how this rule applies to several species of robber flies over time and geographic space. The average color of thorax was analyzed using a spectrophotometer which were used in correlation tests with climate measurements from where the specimen was collected. We found that this ecogeographic rule was not consistent in all four of the species of robber flies that were analyzed. Therefore, other studies of the applicability of ecogeographic rules are being assessed in this genus. This research helps define morphological variation in a widespread species and open avenues for more morphological analysis of insects in a changing climate.

Authors: Anthony Cornwall, Dylan Snook, Travis Ficklin. Mentors: Travis Ficklin. Insitution: Utah Tech University. The purpose of this study was to discover any relationships between individual finger force capabilities of collegiate pitchers and the spin rates and axes of their four-seam fastballs. NCAA Division-I pitchers participated in the study. Using a hand-held dynamometer, multiple trials of a squeezing task were performed by each pitcher. Five trials were made using both index and middle fingers at maximum isometric effort. Then, in counterbalanced order, pitchers also duplicated the isometric squeeze isolating the index and middle fingers in one-fingered squeezes. Pitchers were constrained to make the maximum effort squeezes in a time frame similar to that of delivering a pitch.Spin axis and rate for their four-seam fastballs were taken from previously collected ball flight tracking using a field Trackman system. Analysis was performed for any relationships among axis tilt and relative finger contribution to total force, as well as spin rates with total isometric force. Additional relationships based upon anthropometric measures of the hand and fingers were also explored and the results are to be presented.

Authors: Syrus Miner, Ryland Day, Justin LeClair, Adam Dimaio. Mentors: Randy Klabacka. Insitution: Utah Tech University. Accurate measurement of the knee joint angle is important for diagnosis, treatment, and rehabilitation. With the advancement of wearable technology the measurement of range of motion can now be measured dynamically during exercises. The primary purpose of this research project is to provide a mechanism by which physical therapy outcomes can be improved after knee replacement surgery. This will be accomplished by creating a knee brace attachment that will employ motion detecting sensors to chart the post-op and/or post-injury progression of the movement of the knee joint. It is estimated that approximately 5% of the population over the age of 50 in the United States are currently living with a knee replacement. It has been shown that knee pain has been coupled with depression, low self-esteem, eating disorders, and an overall lower satisfaction in life. Improved knee function has reduced the dissatisfaction experienced in life as daily activities are able to be retained. While physical therapy helps recovery of range of motion for patients, the incentive for patients to complete tasks at home and the lack of standardized data collection may impede patient recovery times. The desired outcome of this research is to create a device that reports the effectiveness of a patient's treatment by using off-the-shelf electronics to accurately measure knee joint range of motion and communicate it to physicians.

Authors: Caroline Nielson, Connor Baird. Mentors: Robert Kagabo. Insitution: Utah Tech University. Opioid Use Disorders Treatment Related Disparities in Patients with Psychiatric IllnessAuthors: Caroline Nielson, Connor Baird, Robert Kagabo, PhD, MSW, MPHAbstractBackgroundThis study is a review of peer-reviewed articles of Opioid Use Disorders (OUD) treatment among individuals with psychiatric illness. OUD is a subset of substance use disorders (SUD) that is chronic and a growing public health concern. There were 47,000 opioid-related deaths in 2018 in the US; OUD is responsible globally for 68% of the drug-related deaths. OUD can result from either prescribed or non-prescribed opioid use. Some populations such as individuals with psychiatric illness have high rates of prescription opioids yet face opioid use treatment and research-related disparities. This review study examines OUD treatment and research-related disparities among patients with psychiatric illness with the goal to improve treatment among this vulnerable population.MethodsUsing PubMed and PsycINFO databases, we performed a search of journal articles regarding OUD treatment which were published between 2010 and 2023. We then completed a review of the journal articles using narrative overview guidelines. All articles reviewed were from randomized controlled trial (RCT) studies. Examples of search terms used include OUD treatment and mental health; OUD and psychiatric illness RCT studies; and opioid use treatment and mental health. Studies included were those of OUD treatment among individuals with psychiatric illness and or with a DSM5 or DSM4 diagnosis. Any studies that did not include OUD treatment and, or a DSM diagnosis, or RCT design were excluded. To avoid duplication, only one journal article from a study was included. ResultsThere were 18 journal articles reviewed that met the inclusion criteria. Treatment periods ranged from 8-24 weeks. Generally, studies excluded individuals with diagnoses such as schizophrenia, schizoaffective disorders, or other serious mental health diagnoses. Several studies included participants with a DSM5 or DSM4 diagnosis of OUD, while excluding individuals with other psychiatric or substance use disorders. Most of the studies used pharmacological treatments and only a few studies had combined pharmacological and behavioral treatments. The common medicines in pharmacological treatments were methadone, buprenorphine, and naltrexone.ConclusionOUD treatment and research related disparities exist among individuals with psychiatric illness, yet this is the population who suffer most from OUD. Additional studies regarding OUD treatment among individuals with psychiatric illness are needed to improve OUD treatments and reduce OUD-related morbidity and mortality rates.

Authors: Hannah Smith. Mentors: Jeff Parker. Insitution: Brigham Young University. The Arabic language uses a rich system of patterns called verb forms to create verbs with related meanings from roots made of consonants. For example, the root k-t-b, in form I, kataba, means ‘to write.’ When it appears in form II as kattaba, it means ‘to make someone write.’ In form III, kaataba, it means ‘to exchange letters,’ ‘to correspond’ (Wehr, 2019). There are ten commonly used verb forms, although not every root is used in every form. As Arabic has been exposed to English and speakers borrow verbs from English into their Arabic, they make choices about how and whether to integrate these foreign words into the verb forms. For example, the verb ‘to block (on social media),’ with the derived root b-l-k, is used in form II, ballaka, ‘to block.’ Other borrowed verbs, however, don’t get integrated in this way. Instead, they are used as a noun with a native Arabic verb, usually ‘amila, ‘to do.’ For example, English ‘to delete’ becomes ‘amila daliit, literally ‘to do a delete.’ This study aims to understand what factors influence when and how completely borrowings from English like these are integrated into the verb forms in Jordanian Arabic (JA) by interviewing university students in Amman, Jordan. The interviews are primarily focused on four forms in which borrowings in JA frequently appear (Salem, 2015). Initial data from a group of eight participants shows a general acceptance for the borrowings in their base forms, with much more variation in speakers’ intuitions regarding their usage in other forms. For example, all eight participants perceive ‘to block,’ ballaka, as a completely acceptable word used by most people. However, only two participants felt the same way about its counterpart form, taballaka, ‘to be blocked,’ whereas two other participants perceived it as something no one would ever use. Furthermore, some participants perceive verbs that rely on ‘to do,’ such as ‘to delete,’ to be in the beginning stages of integration, with three of eight interviewees stating that some of these verbs actually are acceptable in verb form II. Thus far, the perceptions of all the borrowings seem to indicate that speakers believe that integration of borrowed verbs is a gradual process. Furthermore, in addition to other factors, the extent to which the verbs are integrated in JA seems to be affected in large part by cultural perceptions of how foreign the verbs are, a factor that has not been noted in previous studies on the subject.

Authors: Harlan Stevens, Arwen Oakley. Mentors: Stephen Piccolo. Insitution: Brigham Young University. The number of scientific journal articles published per year now exceeds one million. To help maximize the impact of these articles, researchers must ensure that images in the articles are accessible to people with color-vision deficiencies (CVD). Up to 8% of males and 0.5% of females experience at least one form of color-vision deficiency, thus making it difficult for these individuals to discern patterns in images that use particular color combinations. We sought to shed light on this problem by estimating how often published images use color combinations that are unfriendly to people with a CVD. Examining 6,000 images published in biology-oriented research articles published in the eLife journal between 2012 and 2022, we identified images with potentially problematic color combinations. Using quantitative metrics and manual review, we estimate that 13% of these articles would be difficult for people with moderate-to-severe deuteranopia to interpret. We used a convolutional neural network to automate the ability to label images as being problematic for people with moderate-to-severe deuteranopia. The machine learning model successfully classified images in a testing dataset with an auROC of 91.3%. Based on these results, we created a web application that allows users to upload images and view estimates about whether the images are CVD-friendly. Such efforts are critical to ensuring that papers published in the biology literature are interpretable to diverse audiences.

Authors: Adam Call, Parker Myers. Mentors: Mark Hughes. Insitution: Brigham Young University. Petal projections are defined as a special class of knot projection with a single multi-crossing, which causes the formation of loops entering and exiting the crossing. The relative height of the strands as they pass through the multi-crossing is always an odd-length permutation. Recent research has described the complete set of moves which is sufficient to relate any two permutations that describe the same knot. In this talk, we will discuss potential novel knot invariants arising from isotopy of knots and their petal projections.

Authors: Joshua Cook Wright. Mentors: Jordan Jones. Insitution: Brigham Young University. Fifty years ago, Brazilian scholar José de Nascimento Morais Filho rediscovered Maria Firmina dos Reis (1825–1917), Brazil’s first female—and first black female—novelist. Morais published a biography about Reis, including in it as many of her works as he could find. During her lifetime, Reis published the novel, Úrusula, musical compositions, short stories, and poems, though it was only through Morais’ biography that some of these works were reintroduced to the general public. Reis used writing to challenge the flaws she saw in society. The most glaring of these was slavery, with which she had first-hand experience, her Afro-Brazilian mother and African grandmother having been enslaved. Because of this, she has gained notoriety as not only the first female novelist in Brazil, but as an ardent abolitionist. However, the people living in her hometown of Guimarães, Maranhão, knew her—and continue to remember her—quite differently. To them, she was a teacher, whose life’s work was to educate and elevate as many lives as she could. She founded a school that taught both boys and girls and refused to use corporal punishment. Using information gathered on a summer research trip to Maranhão, Brazil, including a visit to the archive where Reis’ works were rediscovered and a visit to Guimarães, I will compare the prevailing understanding of Maria Firmina dos Reis as an abolitionist writer with the powerful and living legacy that I experienced first hand in Guimarães. I conclude by discussing the impact of viewing Maria Firmina dos Reis as both an abolitionist and a teacher, how these views complement each other, and how seeing both sides of her story can help us better understand how activism can manifest in many different ways.

Authors: Macaulay Gould, Chelsea Romney, Andrew Moss. Mentors: Chelsea Romney. Insitution: Brigham Young University. Attorneys play an important role in protecting the rights of the defendant and maintaining fair courtroom outcomes. This project compared self-reported fairness between court customers with and without an attorney representing their case. 372 Provo City Justice Court customers reported their perceived fairness in a 10-item survey, validated by national court assessment tools. Statistical analysis was performed, and a linear regression model revealed no association between legal representation and fairness. This indicates that both groups reported the same level of fairness, regardless of attorney representation. While evidence suggests that court customers with an attorney may receive better outcomes and less jail time, this was not reflected in court customers’ perceived fairness. We suggest a court navigator be available to court customers, so they can better understand how fairly they are being treated.

Authors: Dylan Skinner. Mentors: Mark Hughes. Insitution: Brigham Young University. Deep reinforcement learning (DRL) continues to demonstrate remarkable efficacy in pattern recognition and problem-solving, particularly in domains where human intuition falls short. In the realm of knot theory, an important challenge revolves around constructing minimal-genus slice surfaces for knots of varying complexity. In this presentation, I will outline a novel approach that leverages the power of DRL to tackle this difficult problem. Using braid representations of knots, we train reinforcement learning agents to construct minimal genus slice surfaces by finding sequences of braid transformations that are optimal with respect to a given objective function. This provides a template for attacking other computationally difficult problems in topology and pure mathematics using reinforcement learning.

Authors: Liberty Rausch. Mentors: Kenneth L Alford. Insitution: Brigham Young University. The Hyrum Smith Papers project, patterned after the Joseph Smith Papers, is a scholarly initiative at Brigham Young University aimed at collecting, transcribing, analyzing, and publishing selected extant documents associated with Hyrum Smith, the older brother of Joseph Smith, Jr.—key figures in the early history of The Church of Jesus Christ of Latter-day Saints. With a focus on illuminating the often underappreciated contributions of Hyrum Smith, this project examines a wide range of historical documents. These include, but are not limited to, personal correspondence, journals, sermons, certificates, legal documents, land records, ecclesiastical and administrative documents, and others. The purpose is to contribute to a deeper understanding of Hyrum’s role, leadership, and influence within the context of the American religious, social, and political climate of the early 19th century. The goal of this research is to publish a volume with transcriptions, narratives, photographs, and illustrations associated with Hyrum Smith’s life. This UCUR oral presentation will provide an overview of how relevant documents are identified and incorporated into our corpus.

Authors: Abraham Harris, Juliana Corbridge. Mentors: Mark Hughes. Insitution: Brigham Young University. Braid closures provide a way to study knots and links in 3-space via group-theoretic techniques. Several important open problems in low-dimensional topology can be reformulated as problems in combinatorial group theory via a particular braid invariant called the band rank. In this talk we will describe the band rank and techniques for computing this quantity which draw on tools from reinforcement learning.

Authors: Allen Otterstrom. Mentors: Joe Price. Insitution: Brigham Young University. Data quality is a key input in efforts to link individuals across census records. We examine the extreme version of low data quality by identifying census US enumerators in the US who fabricated entire families. We provide clear evidence of fake people included in the census in Homestead, Pennsylvania. We use the features of this case study to identify other places where there seem to be fake people. Our automated approach identifies census sheets that have much lower match rates to other census records that would be expected, given the characteristics of the people recorded on each sheet.

Authors: Hyrum Veach. Mentors: Amy Harris. Insitution: Brigham Young University. The agricultural laborers’ riots that occurred in England from 1830 to 1831, commonly called the Swing Riots, have been well-studied over the past fifty years. In recent years, historians have sought to better understand individual protests were rooted in local causes and social networks, however, there are still gaps in historians’ understanding of how these events related to urban protests going on around the same time. Through an in-depth exploration into the Norwich weavers’ riots in 1829 and the fires in Swanton Abbott, Norfolk in January 1831 this research attempts to show how the changing relationship between urban and rural communities could link urban and rural protest movements in multifaceted ways. Special attention is paid to the lives of some of these protests’ key participants (Richard Knockolds and the Davison brothers), including their backgrounds, motivations, and the ways their actions were memorialized both in the city and the country. When these two protests are situated in the context of urban-rural relations, it becomes clear that they were part of an active renegotiation of the relationship between city and country brought on because the pressures of industrialization were pushing the two together in ways that many found threatening. Each of the people involved had a different vision for what this relationship should look like, as well as a vested interest in ensuring this vision prevailed. By foregrounding how these participants approached the boundaries between urban and rural worlds, this research emphasizes the ways this boundary was a crucial point of contention in early industrial Britain.

Authors: Samantha Richardson, Sam Simmons. Mentors: Mark Hughes. Insitution: Brigham Young University. During cellular mitosis long DNA molecules are first split and then separated with one copy being transferred to each of the daughter cells. A class of enzymes known as topoisomerases assist in the separation of the two strands of DNA, though the precise mechanism by which they work is unknown. In this talk we will discuss attempts to model topoisomerase action using techniques from reinforcement learning.

Authors: Elisabeth Morris. Mentors: Desirée Oliveira. Insitution: Brigham Young University. The purpose of this study is to better understand what it means to be an American by examining perceptions of national identity through the lens of Brazilian Americans living in Northern Utah. With this purpose in mind, we hope to add to the discussion of national identity during a time when the United States' title as a “melting pot of nations'' is called into question. The study focuses on the question: How do Brazilian Americans living in Northern Utah perceive their own national identity? Additionally, it seeks to answer the following subquestions: Does a pluralistic national identity exist among Brazilian Americans in Northern Utah?; If a pluralistic national identity exists among Brazilian Americans in Northern Utah, do the two identities coexist, or is there a synthesis of the identities and what does that look like?; How do they perceive American national identity?; How is American National identity influenced by Brazilian Americans in the United States? Working with local Brazilian businesses, cultural groups, families, and individuals, we collected data through a variety of ethnographic methods including participant observation, semi-structured interviews, mapping, sonic recordings, oral histories, photo, video, and media usage. We then used thematic qualitative data analysis methods, finding that Brazilian Americans in Northern Utah see their identities as pluralistic as well as that Brazilian and American identities coexist, operating in different spaces. The results show how American national identity is fluid and complex, existing as a companion to other identities, demonstrating how “Brazilianess” contributes to “Americanness” by creating more empathy, understanding, and union in the United States.

Authors: Sven Wilson, Joseph Price, Hannah Hammond, Katharine Wilson. Mentors: Joe Price. Insitution: Brigham Young University. Research suggests that environmental and behavioral risk factors often shared by spouses will result in similar spousal lifespans. This is seen most powerfully in the well-documented tendency for the hazard of mortality to rise immediately after spousal loss, thereby causing a greater concordance in spousal lifespans. This is sometimes referred to as "broken heart syndrome," and a widowhood effect on mortality has been observed in multiple large population studies in many countries. We employ a new dataset from the United States containing over 2.8 million couples drawn from the 1940 census who were born between 1880 and 1920 who are linked to US Census Schedules through 1940 and to the wiki-like genealogical data source Family Search. To account for the presence of assortative mating, we employ a strategy where the lifespan of the spouse's siblings is used as an instrumental variable for spousal lifespan. We find only a small amount of assortative mating on the basis of lifespan, but considerable sorting based on education and income. Our simple IV model finds a statistically significant causal relationship between the death of both husbands and wives on the lifespan of their respective spouses, and those effects are roughly twice as big as the OLS estimates. An additional year of life for the spouse adds .11 years to the husband’s lifespan; and .157 years to the wife. The larger effect on women’s lifespan is in opposition to most of the literature on the widowhood effect.

Authors: Spencer Paulsen. Mentors: Jeffery Tessem. Insitution: Brigham Young University. Today approximately 537 million adults are living with diabetes. This figure is expected to rise to about 640 million by the year 2030. Metabolic stress is known to drive the development of the disease. The pancreatic β-cell which is responsible for producing and secreting insulin in response to elevated blood glucose concentrations is particularly sensitive to metabolic stressors. Therefore, understanding the molecular character of β-cells is important to curing diabetes. Nkx6.1 is an important β-cell transcription factor critical for proper β-cell development and function. Our lab has shown a potential interaction between Nkx6.1 and PACS1 in β-cells. PACS1 is associated with the Trans-Golgi network and is involved in protein maturation by sending newly synthesized proteins to their intended target inside or outside the cell. We know that insulin is synthesized in the ER and sent to the Golgi for vesicle packaging. We hypothesized that PACS1 helps sort insulin in the Trans-Golgi network, and that interaction with the β-cell transcription factor Nkx6.1 is essential for this process. Therefore, the study of the interaction between Nkx6.1 and PACS1 may provide a greater understanding of diabetes and insulin secretion in β-cells. Here, we present the results of our investigation of the link between Nkx6.1, PACS1, and the Golgi apparatus. We present data validating the Nkx6.1-PACS1 interaction, the cellular location of the interaction, and the effect of changing glucose concentration on this interaction. These results will help deepen our understanding of how the Nkx6.1-PACS1 interaction works to ensure proper β-cell function.

Authors: Peyton Jackson. Mentors: John Chaston. Insitution: Brigham Young University. This study explores the relationship between seasonal variations and the development rates of Drosophila Melanogaster. Flies are closely monitored in a controlled laboratory setting after being transferred from outdoor cages across different seasons. In the outdoor cages, the introduction of the bacterial strain A. orientalis DmW_045, commonly referred to as '45,' enhances development rates and ensures consistent data collection. Axenic flies, created by thoroughly sterilizing fly eggs with bleach to eliminate external bacteria, are employed to isolate genetic influences on the relationship between season and development rates. The core methodology involves two groups: axenic flies treated with '45' and a control group unexposed to '45.' This approach guarantees data accuracy and assesses development rates based purely on genetics. Development rates are measured by marking eclosed (hatched) pupae on vials at specific intervals. The research seeks to unveil the correlation between seasonal variations and fly development rates, shedding light on the role of seasonal factors in shaping development. This study explores the interplay between seasonal variations and Drosophila fly development, emphasizing genetics and environmental factors. Its potential significance lies in providing insights beneficial for ecological and genetic studies, potentially offering a clear genetic link between seasons and development rates that can enhance our understanding of the natural world.

Authors: Abigail Cheever, Chloe Kang, Hunter Lindsay, Mackenzie Hansen, Kim O'Neill, K Scott Weber. Mentors: K Scott Weber, Kim O'Neill. Insitution: Brigham Young University. Chimeric Antigen Receptor (CAR) T cell therapy is a modern technology that has become a widely accepted treatment for blood cancers such as lymphoma and leukemia. Recent studies have proven the successful application of CAR T cell therapies in autoimmune diseases as well. Graves’ Disease (GD) is an autoimmune disease that affects approximately 1 in 100 Americans and is the most common cause of hyperthyroidism. GD is mediated by anti-thyroid stimulating hormone receptor (TSHR) antibodies produced by autoreactive B cells. Our hypothesis is that a curative treatment for GD can be created by designing a CAR T cell that specifically targets the autoreactive B cells in GD, by using TSHR as a binding domain to act as bait for the anti-TSHR B cells. We selected TSHR epitopes for the binding domain of the CAAR T cell, and our anti-TSHR antibodies bound significantly to our engineered CAAR T cells. A target B cell line with anti-TSHR B cell receptors was engineered using the Nalm6 B cell line. Using primary human CAAR T cells, activation and cytotoxicity assays against anti-TSHR B cells shows that CAAR T cell therapy is an effective and promising method to treat antibody mediated autoimmune diseases like GD.

Authors: Ellie Martin. Mentors: Cynthia Finlayson. Insitution: Brigham Young University. Pottery reconstruction allows archaeologists to better understand their artifacts and gain a more complete understanding of ancient ceramic techniques. Reconstruction techniques have evolved over time that strive for both better conservation practices and long-lasting results, as well as the use of bonding materials that are reversible rather than permanent. This poster examines the process of utilizing an acetone-based glue consisting of dissolved polymer beads of differing strength solutions. The entire process with be illustrated by step-by-step photos and directions as well as the quality of the final results. Two layers of different solution consistencies are applied to the pottery in different stages before the pieces are joined together. The first layer of glue is a thin coat made of 5% polymer and 95% acetone, and it is applied to any edges that will be refit. This layer fortifies the sherd and ensures that the glue applied is not stronger than the sherd itself. After the first layer has dried, the second coat of glue, made of 12% polymer and 88% acetone, is added to each piece. The sherds are then joined and placed in a secure location to dry, such as a bin of silica beads. Acetone-based polymer glue is strong and flexible, yet also dissolvable. Any mistakes can be unmade with the careful application of small amounts of acetone, and no restoration action is utilized on the pottery fragments that cannot be undone. This method of reconstruction is safe, secure, long-lasting, and meets modern conservation standards.

Authors: Lant Jenkins, Dalyn Davis, Caleb Kratt. Mentors: Jeffery Barrow. Insitution: Brigham Young University. Currently, nematodes, fruit flies, and zebrafish are effective models for gene manipulation due to their rapid embryonic development and capacity for producing a large number of offspring. Avians such as the chicken have also served as embryonic models and have proven to be powerful tools for exploring developmental processes such as neural tube or limb development. Because of high chick fecundity, a relatively short period of development, and the accessibility of the chick embryo, the chicken would make an excellent model for gene manipulation that would significantly enhance our study of the relationship between genes and phenotypes in vertebrates. The aim of our research is to develop a line of genetically modified chicken germ cells into which new DNA vectors can be seamlessly introduced via cassette exchange. The design of the germ cells is such that the DNA vectors will be incorporated into the chicken genome at specified safe harbor locations, areas of euchromatin where the introduced DNA will not interfere with other genes. These modified germ cells can then be injected into chicken embryos and contribute to the chick’s germline. We anticipate that this model for modifying the chicken’s genome will significantly enhance our ability to introduce new DNA vectors, making the chicken a more desirable model for studying embryonic development via genetic manipulation.

Authors: Dustin Edmonds, Devin Needs, Riley Fisher. Mentors: Brent Feland. Insitution: Brigham Young University. Research questionHow does localized vibration increase blood flow? We know that localized vibrations increase blood flow. We wanted to know if this is done through the release of hormones, specifically histamines. Relevant research contextResearch on blood flow has been done using whole-body vibration but research on the effects of localized vibration on arterial blood flow is scant. To date, there are only a couple of research articles on massage guns despite their prolific use in the sports industry. No research to date has attempted to assess the mechanism behind the increased blood flow that results from localized vibration.MethodsTwenty-one participants completed this study, 11 males and 10 females, mean age of 22.1 +/- 2.0 years. The mean male height 181.3 +/- 9.6 cm and weight 80.7 +/- 19.0 kg. The mean female height is 169.2 +/- 7.5 cm, and the mean weight is 64.5 +/- 9.3 kg. All subjects were “recreationally active” and exercised at least three times a week for thirty minutes a day. Excessive activity or high-level athletes were not allowed to participate. Each subject received localized vibration to the gastrocnemius (vibration of 47 Hz for 10 minutes) with and without an antihistamine drug by reporting to the lab on 2 separate days. The non-antihistamine trial was performed first, followed at least 24 hours later by ingesting an antihistamine (180 mg of fexofenadine) 1 hour before the trial. Blood flow in the popliteal artery was measured using an ultrasound. Measurements (mean and peak blood velocity, volume flow, popliteal diameter, and heart rate) were taken before localized vibration treatment and then taken at intervals for 19 minutes after the treatment.AnalysisWe used a cell-means mixed model to statistically compare the effect of vibration on blood flow with and without antihistamines. This was done by evaluating the blood flow response immediately post vibration and comparing that to baseline values for both control and antihistamine conditions. Results and ConclusionThere was a significant increase in blood flow without antihistamine while administration of antihistamine blunted the blood flow response and resulted in an insignificant increase in blood flow. In the tests with the antihistamine, we saw an insignificant change in blood flow immediately post vibration, resulting from the localized vibration. From this experiment, we hypothesize that increased blood flow from localized vibration is due to activation of mast cells which release histamines and that this cellular activation is force dependent.

Authors: Eric Morris. Mentors: Jerry Johnson. Insitution: Brigham Young University. Black Spot Disease (BSD) is a parasitic infection that occurs when trematode parasites burrow into the skin of a fish and form a cyst. The infected fish releases black pigment around the cyst creating a visible black spot. Fishes can have different levels of infection ranging from a few cysts located on their skin to an entire body speckled with these markings. Parasitic diseases typically come with a cost to the host species. Little research has been conducted examining the cost of such diseases in the placental live-bearing fish Brachyrhaphis rhabdophora. My study examines the influence of BSD on female association preferences by providing female Brachyrhaphis rhabdophora dichotomous choices between males who appear to be healthy, slightly infected, moderately infected, and highly infected. The different levels of infection in the males are simulated by ink tattooing.

Authors: Naavah Craze, crazen@byu.edu . Mentors: John Chaston. Insitution: Brigham Young University. Wolbachia are maternally inherited bacteria found in the reproductive organs of many arthropod species. These parasitic bacteria are influential in the reproductive fitness of the host and can affect a population’s male-to-female ratio. Wolbachia can only be cultured in living cells and current methods for inoculating hosts with this bacteria have low success rates. These factors make these bacteria difficult to study in lab environments. In this project, we test a pricking method for inoculating Drosophila melanogaster with Wolbachia. In this method, axenic, or bacteria-free, female flies will be pricked in the abdomen with a Wolbachia-coated needle. Eggs from these flies will be collected from 3 to 5 days after pricking and will be incubated. The offspring will be tested using gel electrophoresis to see if inoculation was successful. If successful, this method can be used as a simple and efficient way to introduce Wolbachia into Drosophila melanogaster. These inoculated flies can then be used in experiments to study the relationship between the bacteria and the host organism.

Authors: Lauren Heath, John Chaston. Mentors: John Chaston. Insitution: Brigham Young University. Mounting evidence shows that gut microbiota disruption is related to several physiological processes and disease development in mammals; however, the mechanisms by which these processes take place are not fully understood due to the complexity of the mammalian gut microbiome. Studying the fruit fly, Drosophila Melanogaster proves to be a valuable model for understanding the gut microbiome on a smaller scale. Previous research has identified microbiota disruption alters the fly’s ability to cope with thermal stress and causes slower chill coma recovery times. Chill coma recovery is an important survival trait and stress response in Drosophila flies. Microbiota may affect the ability of hosts to tolerate stressful situations. The purpose of this project is to determine the effect of different bacterial strains on the mechanisms of chill coma recovery through a metagenome analysis of different bacterial strains. I hypothesize that certain microbe genes will increase chill coma recovery times. To test this assumption, I will mono-associate by flooding different groups of eggs with a microbe wash and infusing their food with the same bacteria strain. After cultivation, a chill coma will be induced by placing mature adult flies in empty vials on ice for three hours, and then the time for each individual to stand up after their transfer to room temperature will be recorded. Subsequently, I will assess cold tolerance, and evaluate the rates of recovery. With this, we can further identify the mechanisms of the chill coma recovery process by pinpointing specific proteins necessary for this process through a metagenome-wide association approach (MGWA) and gene analysis. If I observe no effect of the bacterial strains on chill coma recovery times, I can test different mixes of microbes to determine if improved recovery rates are a side effect of global metabolic changes caused by decreased biodiversity in the gut of drosophila flies. Studying the metabolic processes of Drosophila allows us to unravel further the mechanisms by which gut microbes affect the fitness and health of their host. My findings may be extrapolated to further understand the human gut microbiome.

Authors: Jonathan Dickerson, Christian Ahrens, Zach Valentine, Sara Linderman, Hillary Wadsworth, Lauren Ford, Jordan Yorgason. Mentors: Jordan Yorgason. Insitution: Brigham Young University. The nucleus accumbens (NAc) is a major component of the reward pathway in the brain. The role of ATP as a neurotransmitter within the NAc has largely remained unstudied. Multiple experiments have hinted at larger roles ATP may have in other areas of the brain, but not yet in the NAc to our knowledge. Our goal was to characterize ATP release in the NAc, in an attempt to pave the way to finding novel methods for treating/preventing addiction. Fast-scan cyclic voltammetry (FSCV) was used to track ATP and dopamine release in the NAc of mouse brains, evoked with electrical stimulations before and after application of various drugs. Blocking nAChR’s and NaV channels reduced both ATP and dopamine release. Blocking DAT’s enhanced release, but blocked clearance of both ATP and dopamine, and activating the immune system with LPS increased release of both ATP and dopamine. Changes in dopamine and ATP release after drug application were definitely correlated, but not proportionally so. While in some instances, the same mechanisms previously thought to work just with one neurotransmitter or the other had effects on both, changes in ATP signaling cannot be fully explained by dopaminergic machinery in the NAc.

Authors: Byron Adams, Elias Johnson, D Alejandro Espinosa-Lucas, R Darío Perez-García, Israel Solano-Zavaleta, J Jaime Zúñiga-Vega, Gonzalo A Ramirez-Cruz. Mentors: Byron Adams. Insitution: Brigham Young University. Urbanization has forced animals to respond to novel environments. One of the challenges of urban ecology is that it is unknown if the ecological processes that take place in wild conditions also occur in urban environments. In natural ecosystems, key predators can limit prey abundance and have a widespread effect on trophic levels. Here, we examine a predator-prey interaction in Mexico City, an urban ecosystem where prey can be evaluated in the presence or absence of apex predators. Nearly all original ecosystems in Mexico City have been converted into man-made structures. Many of the green spaces in the city contain populations of the Mexican gray squirrel. These green spaces are also inhabited by different species of raptors, the most frequent being Harris’s hawk, Cooper’s hawk, and sharp-shinned hawk. Little is known about interactions between raptors and squirrels in terms of demographic parameters. We do know, however, that raptors prey on squirrels. We predicted that in parks where raptors were present, there would be fewer squirrels than in parks where there were no raptors. We studied the relationship between raptors and squirrels using occupancy models to determine the environmental factors that affect the presence of both squirrels and raptors. We then tested if the presence of raptors influenced the occupancy probability of squirrels. Lastly, we estimated the abundance of squirrels in parks, both where raptors are present and where they are absent. Contrary to our predictions, we found no evidence that predators predict squirrel abundance. However, we did find evidence that the presence of raptors can be predicted by the abundance of squirrels. These results suggest that this predator-prey interaction in Mexico City parks could be relatively recent and still not in equilibrium, or that other factors may be affecting the dynamics between these two urban populations.

Authors: Kirsten Sanders. Mentors: Ruth Kerry. Insitution: Brigham Young University. The Great Salt Lake (GSL) found in the Salt Lake Valley is a remnant of Lake Bonneville. In the 1980s the GSL reached record high water levels. But by 2022, the lake has been drying up and reached record-low water levels. Previous research using seven years of Purple Air Network data has shown that particulate air pollution levels, PM 2.5 and PM 10, are worse within 10km of the Great Salt Lake. Other research investigating the distribution of toxic heavy metals (Copper, Arsenic, Lead, and Zinc) in topsoil samples from throughout the Salt Lake Valley showed that some of the highest levels of Copper and Arsenic pollution were found near Saltair among dried sediments on the shoreline of the Great Salt Lake. This study involved collecting numerous samples from the dried shoreline of the Great Salt Lake and analyzing them for their heavy metal contents using Xray Fluorescence. The heavy metals were then mapped and linked to sediment particle sizes and pH to indicate where the most toxic sediments are located, and where the sediments that are most easily picked up by the wind are located. The maps of heavy metals will be used with our analysis of the Purple Air Network and wind rose data from our previous study to show where toxic dust clouds are most likely to be an issue to the population. Links with 2016 Asthma ER visits and Hospitalizations will also be investigated.

Authors: Eva Driggs, Megan Cahoon, Seth Bybee. Mentors: Seth Bybee. Insitution: Brigham Young University. Odonates (dragonflies and damselflies) exhibit diverse coloration. These vibrant colors are important for species recognition, camouflage, and mate recognition. Adult odonates emerge in late spring, mate and reproduce, and die by fall. As color and seasonality are both important aspects of the adult stage in odonates, we pose the question: Does the odonate community color composition change over the season?We hypothesize that different colors within the odonate community will peak in abundance at different times in the season. We conducted population estimates weekly at a local park in Provo and analyzed data from Utah, the Great Basin, and the West Coast (GBIF). From this data, we found that generally, blue odonates have the highest abundance throughout the season, but red odonate populations persist later in the season while blue populations decline which may be due to seasonal changes in heat and light.

Authors: Cooper Johnson. Mentors: John Chaston. Insitution: Brigham Young University. The main goal of this project is to determine the relationships between microbes in the microbiota of Drosophila and the activity rate of Drosophila at low temperatures. Previous research has already determined that the microbiota leads to changes in fly activity levels at normal temperatures (Schretter, 2019). Studying the relationship at low temperatures with the method I will outline below will help us to have a more holistic view of how the bacteria affect fly locomotion and behavior. This holistic understanding can open doors for other projects that study if manipulation of the microbiota could have a specific desired effect on the fly’s behavior, and it can help us understand how the microbiota’s impacts are affected by drastic changes in temperature. In this experiment we seek to determine how each of 41 different strains of bacteria affects fly activity at temperatures of approximately 17 degrees celsius using several different metrics to measure both locomotion and social behaviors. These metrics would be collected simultaneously by placing flies in a chamber for Drosophila Video Tracking (DVT), which would use a camera and tracking software to record the above-mentioned metrics on fly activity. Using so many different metrics allows us to have a more holistic view of the flies’ activity and to be able to better determine exactly how the bacteria are affecting the flies. In order to connect the data with the microbiota, the flies would be separated into 41 different experimental groups and each group would be “mono-associated” with one of the 41 different above-mentioned bacterial strains. Mono-association means that each of the fly eggs would be bleached, and then divided in groups, with each group being grown in an environment containing a specific type of bacteria. This means that each fly experimental group would have one species of bacteria in their microbiota, and therefore we can associate changes in fly behavior to the differences in the Drosophila microbiota. Having determined which bacteria affect the fly activity at low temperatures, we would use Metagenome-wide association to determine which bacterial genes are causing this change. Metagenome-wide association involves comparing the genomes of bacteria shown to cause changes in Drosophila activity in order to identify the similar protein-coding sequences that may be the causes of these changes in activity. To confirm the findings at that stage, the genes are expressed in a bacteria that doesn’t express them normally, and flies are analyzed to see if the changes in activity persist. If they do, we can safely say that the changes are due to that specific gene sequence. We expect that the mono-associated flies will show differences in activity level, because it has already been established in a previous study that the microbiota in the flies has a statistically significant effect on the activity level of the flies, meaning that the presence of certain bacterial cultures in the flies lead to a difference in activity. Therefore, if there is no difference in activity level, it is most likely due to the low temperatures, and it may then be necessary to perform the experiment again at different temperatures to determine at what point the temperature negates the effect caused by the bacteria present in the fly microbiota. We aim to study the relationship between the microbes present in the fly microbiota and their activity level at low temperatures and identify the mechanisms by which the microbes affect the fly activity level.

Authors: Nolan Rodeback, Jerald Johnson. Mentors: Jerald Johnson. Insitution: Brigham Young University. The environment plays a crucial role in the development and evolution of organisms. In some cases, the environmental pressures on a mother may result in changes of offspring phenotypes. This phenomenon, called maternal effects, might occur in the tropic fish species Brachyrhaphis rhabdophora. I will conduct an experiment to test if maternal effects can be induced by the presence of a predator. To measure this we will look at maturity age and growth rates in the offspring where mothers are reared in the presence or absence of predators. If predators have an effect, offspring should mature at earlier ages and have faster growth rates than individuals with mothers reared in the absence of predators.

Authors: Brooklyn Jones. Mentors: Bradford Berges. Insitution: Brigham Young University. Staphylococcus aureus (SA) forms biofilms that cause considerable morbidity and mortality in patients who receive implanted devices such as prosthetics or fixator pins. An ideal surface for such medical devices would inhibit biofilm growth. Recently, it was reported that surface modification of stainless-steel materials with carbon-infiltrated carbon nanotubes (CICNT) inhibits the growth of SA biofilms. Through previous research, we investigated this antimicrobial effect on titanium (Ti) materials with CICNT coated surfaces in a variety of surface morphologies and across a broader spectrum of SA isolates. We have in vitro evidence that carbon nanotube-treated materials are effective at preventing SA biofilm formation on Ti, the most common material used in human implanted devices. We now wish to study the effects of carbon nanotube treatments on prevention of SA biofilm formation in vivo which has potential value to improving human health .

Authors: Wesley Hendricks. Mentors: Steve Johnson. Insitution: Brigham Young University. Pioglitazone Hydrochloride (PGZ) is a well-accepted treatment of type-II diabetes and has been shown in previous studies to increase lifespan of C. elegans. The agonist has been shown to affect the insulin, IGF-1 signaling pathway (IIS), dietary restriction (DR) and germline signaling pathways. Previous studies have not been able to identify epigenetic marks that are a result of the pathway effects of PGZ (Jia Wenguan et. al). This study seeks to understand how subsequent generations are affected by antiaging compounds and what epigenetic marks are transmitted through the germline of C. elegans from these three pathways. After exposure, through lifespan tracking, we hope to see a change in transgenerational longevity. Using RNAi, we then plan to knockout known genes in each pathway. We hope to see that intestine-germline pathway and maintenance genes that are known to function in each pathway are required for the longevity effect.

Authors: Julia Gilpin, Lucy Bowden, Sela Harris, Elisa McRae, Jenny Bean, Brooklyn Jones. Mentors: Bradford Berges. Insitution: Brigham Young University. Staphylococcus aureus (SA) is a significant human pathogen that has acquired antibiotic resistance, posing a global health threat. This bacterium is prevalent in livestock, raw meat, and healthcare settings, leading to infections ranging from mild to life-threatening. The emergence of multi-drug resistant strains, such as Methicillin-Resistant Staphylococcus aureus (MRSA), further complicates treatment. The mechanisms of antibiotic resistance transfer, especially within biofilms, remain poorly understood.This research aims to investigate horizontal gene transfer within biofilms of SA isolated from humans. Leveraging the work of the Berges laboratory and techniques used by other researchers. The experimental design involves the use of heat-killed cells as donors to prevent conjugation, shedding light on how biofilm structure influences transformation efficiency.To address the hypothesis that biofilms serve as hotspots for horizontal gene transfer, the research employs a two-component system for upregulating natural competence genes in SA under biofilm growth conditions. This approach will allow us to detect natural transformation, advancing our understanding of SA infections closely associated with biofilm formation. If successful, this study will provide insights into how SA acquires antibiotic-resistant genes and may lead to the development of preventive measures and treatment strategies to combat drug-resistant strains.

Authors: Levi Pouwer, Keaton Helquist. Mentors: Jared Nielsen. Insitution: Brigham Young University. OBJECTIVE: To identify brain regions and networks underlying acquired-brain-injury-induced insomnia. BACKGROUND: In patients of tertiary-level mental healthcare facilities, symptoms of chronic insomnia are prevalent in 78.2% of the population. Additionally, among those who suffer from acquired brain injury, few symptoms are as generally pervasive as that of chronic insomnia. Meta-analyses of insomnia patients with fMRI data have failed to identify consistently affected brain regions. Individual studies have suggested several brain regions are involved in insomnia, including the anterior cingulum, orbitofrontal cortex, the insula, caudate nucleus, and the anterior capsula interna. However, few regions have consistently been implicated in the pathophysiology of insomnia. Moreover, little is known regarding the collective brain networks involving insomnia. DESIGN/METHODS: A comprehensive systematic literature review was conducted to identify case studies of individuals afflicted with acquired brain injury-induced insomnia (N=53). The review process was facilitated by employing Cadima, specialized literature review software. To establish a reference baseline for comparison, a substantial cohort of resting-state functional MRI (fMRI) scans from healthy control subjects (N=1000) was meticulously acquired. Subsequently, lesion network mapping analysis, as outlined by Fox et al. in 2018, was applied to the lesions observed in the 53 patients. This method allowed us to assess the impact on functional connectivity and analyze its implications by comparing the patients' lesion networks with the baseline scans obtained from healthy controls. RESULTS: In the context of lesion network mapping analysis, our study revealed that 45 out of 53 patients diagnosed with acquired brain injury-induced insomnia exhibited brain lesions that were functionally connected to both the left amygdala and the left anterior insula. Moreover, in 39 out of 57 patients, the lesion networks also included bilateral anterior insula and the hypothalamus. Comparative analysis of these lesions with a normative database unveiled a substantial disparity in functional connectivity to the left amygdala and left anterior insula in patients suffering from acquired brain injury-induced insomnia. This finding underscores the relevance of tissue within these regions and tissues functionally connected to these areas in relation to the onset of lesion-induced insomnia. CONCLUSION: The observed functional network connectivity among the left amygdala and the left insular region in relation to insomnia introduces a potentially more intricate mechanism underlying the onset of insomnia, in addition to the previously proposed factors such as hyperarousal in cortical regions and subcortical neuropathologies. Our findings highlight the possibility of a deeper connection between the initiation of insomnia and limbic structures, specifically emphasizing their role in emotion and sleep regulation. This suggests that the neural mechanisms influencing anxiety may have interrelated effects on the mechanisms influencing insomnia. It is evident that further investigation, particularly among psychiatric patients afflicted with insomnia, is warranted to delve into the activities occurring in these regions and to elucidate the potential interplay between anxiety and insomnia. This research paves the way for a deeper understanding of the complex relationship between neural pathways and the development of insomnia, ultimately offering valuable insights for future clinical and therapeutic considerations.

Authors: Connor Hough. Mentors: Johnny Chaston. Insitution: Brigham Young University. Many variables can have an effect on the microbiota (microbial community associated with an organism) which can, in turn, affect the health and lifestyle of the microbiota's host organism. One such variable is geographic latitude (distance away from the earth's equator) which was the subject of a portion of a study performed by Walters et al. in 2020 and a similar study performed by Henry et al. in 2022. Walters et al. determined that latitude had a significant effect on the composition of the gut microbiota in Drosophila melanogaster (fruit flies) while Henry et al. determined that latitude did not have a significant effect. To better understand what variables may have caused a difference in the results of these two studies I plan to perform data analysis on the data provided from both Walters et al. and Henry et al. Particularly, Henry et al. provided data about the microbiota of other sample types associated with D. melanogaster across the latitudinal cline such as the fruit fly excrement, the leaves in their environment, and their diet which consisted of apples and grapes. While this data was mentioned in Henry et al.’s paper I feel that a more in depth analysis of these sample types could bring insight about how these microbiota affect one another and how latitude affects each of them separately. I will use QIIME (a bioinformatics data analysis software) data analysis methods, such as alpha and beta diversity metrics, and R to analyze the data provided and create graphs. Because each sample type in the data was recorded with unequal sample sizes I will also need to make a new taxonomic graph of the sample types when measured proportionally to each other. To do this I will use QIIME to group the original feature table metadata from Henry et al. by sample type and return a new table with an equal number of samples for each sample type. With this done I will then be able to make a new bar plot using QIIME. I will then use this new set of adjusted data to analyze the relationships between latitude and each sample type as well as the relationship between each sample type to one another. I will also perform these data analytics methods on the data provided on fruit fly gut microbiota by Walters et al. and compare the results with the fruit fly gut microbiota provided by Henry et al.. I hypothesize that further analyzing the data from these sample types will reveal new correlations between the microbiota of these sample types and with latitude. If no new information is learned through this analysis then it will solidify Henry et al.’s claim that latitude does not significantly affect the microbiota. Alternative outcomes may show that there is not a correlation between the microbiota of sample types which would indicate that the microbiota exhibits a neutral behavior and is not selective. However, if my hypothesis is correct then these correlations would show that the microbiota of separate sample types are related to the microbiota of other sample types and that latitude does have a significant effect on the microbiota of each sample type. The results of this analysis will open up more pathways for study about the reason behind these correlations or the lack thereof.

Authors: Becca fabis. Mentors: Alexandra Giannell. Insitution: Utah Valley University. On December 2nd my friend was killed by a hit and run driver while going on a run at night. It was right before finals. I was involved with the police and helped her family pack up all of my beloved friends belongings and watched as her daddy cried in her room. Words can’t express the agony I saw. Two months later I learned that another roomate (in a different apartment) her cousin had committed suicide. And we were the place everyone gathered. I held her cousin’s (who committed suicide) sister in my arms as she cried. Words cannot express the agony I felt for them.Then on the first day of school this semester (a month ago) my father suddenly passed away from a brain aneurysm. He was at the peak of health. My mom puked from sickness and shock, later got sick with other illnesses, (she’s doing a little better now) then my sister got sick and I was left to care for them on my own. There’s no words to express my exhaustion. The late nights of wandering my house making sure we were safe. Words have no ability to express my agony I’m in. After my roomate passed I created a painting. It’s called my grieving process because it’s of a house, the house represents me. In it through color you can see the process that grief can have on a person. I’m only 25, people say I’m too young to lose this many people. Perhaps they’re right. It’s an eerie painting and pretty odd when you look at it. I have had now three losses that have taught me that sometimes words aren’t enough. That it’s through art I can scream and it is documented. I would love to present on how creating art can be the breathe of fresh air one desperately needs in order to continue on in this fight we call life.