2020 Abstracts

Leavitt, Amanda; Harris, Heather; Szanter, Kathryn; Stokes, Alexis (Weber State University)
Faculty Advisor: Dunn, Charles (Weber State University, Child and Family Studies); Hubler, Daniel (Weber State University, Child and Family Studies); Osai, Keith (Weber State University, Child and Family Studies)

How a child is parented can influence their success socially, mentally, physically, and emotionally. Agarwal (2017) states that "Parenting plays the most important part in any child's life, it can help a child by teaching them to deal with people, situations and adapt to a better living standard" (p.1335). Parents help form worldviews, shape a child's attitude towards personal achievement, teach how to approach adversity in life, and satisfy their needs whether it be psychological and/or physiological. Much is known about how the base of one's childhood is built upon one's primary caregivers' parenting style, however, less is known about the intergenerational transmission of parenting philosophies.

The current study was designed to assess how parenting styles affect child delinquency and bullying. Through social media invitations and snowball sampling, 200 people accepted invitations to participate in an online survey incorporating both open-ended and quantitative items. Participants were asked to reflect on how they were parented and then state what they have maintained or changed in their own parenting styles. Considering the rich nature of the responses to the qualitative items, a corroborative narrative came together through personal stories.

Through thematic analysis, several themes emerged. When participants reported being parented with: open communication, flexibility, love, trust, and higher expectations they wanted to keep those traits. When asked what participants carried on from their parents one participant stated, "Cherish the family and the moments we have together." Additionally, 28% (n = 113) of our participants shared the importance of spending quality time together. However, when participants' parents did not display those traits, most participants indicated that similar traits, namely communication, flexibility, love, quality time, trust, and higher expectations were desired in their homes. The findings from the current study can be used to inform future parenting research assessing influence on subsequent generations.

Miriam Reber; Ethan Del Toro; Holden Lyman; Weston Hargis; Travis Ficklin (Dixie State University)
Faculty Advisor: Ficklin, Travis (Dixie State University, Health and Human Performance)

In recent years sport climbing has increased in popularity, and with that have come questions regarding what factors improve performance. While multiple studies have examined the effect of anthropometric factors on injury in climbing, few have examined their relationship with climbing performance. Therefore, the purpose of this study is to compare the anthropometric measures of high-level to intermediate level climbers to determine if certain body types, or dimensions, lend themselves to better performance in climbing.

Twenty-one subjects were divided into an intermediate (INT) or high-level (HL) group based on highest graded climb achieved. Various measures including height, limb lengths, and experience. These were compared between the two groups using t-tests.

Significant differences were found in the number of days climbed per week (HL 2.9±1.1, INT 2.17±0.6, p = 0.03), and the ratios of upper arm to full arm length (HL 0.44±0.05, INT 0.41±0.02, p=0.03), and trunk to height (HL 0.26±0.03, INT 0.23±0.01, p=0.01). There were also statistical trends towards years of experience (HL 5.46±3.8, INT 3.39±1.8, p = 0.06), and trunk length (HL 44.96±7.1cm, INT 41.28±2.7cm, p=0.06).

The current data suggest that for the levels of climbing examined (5.9-5.13b on the Yosemite Decimal Scale) a greater trunk to height ratio, greater upper arm to arm length ratio, and increased frequency of practice (a variable within the climber's control) may positively impact climbing ability.

LeBaron, Josh; Obray, J Daniel; Steffensen, Scott (Brigham Young University)
Faculty Advisor: Steffensen, Scott (Family, Home, and Social Sciences, Psychology)

Dopamine neurons in the substancia nigra (SN) and ventral tegmental area (VTA) are inhibited by dopamine (DA) via dopamine 2 receptor (D2R) activation. D2R expression in the striatum is a well-known biomarker for brain DA levels, drug abuse, and dependence. Markers of D2R expression are not only detectable in the brain but are also expressed in peripheral tissues, including the blood, where DA appears to play a pivotal role in mediating communication between the nervous and immune systems. Alteration in lympocytic D2Rs are seen in chronic psychostimulant use (Ersche et al., 2011). For the last two decades it has been generally accepted that D2R expression in the striatum is reduced by chronic ethanol use. Additionally, research has suggested that these changes mirror changes in DA levels in the striatum and predict risk of relapse. Despite this, the timecourse over which these changes occur has not been demonstrated. Further, recent research has challenged both the reduction in D2R expression produced by chronic ethanol and the mechanism whereby it was believed to be produced (reductions in striatal DA levels). This research has suggested that alterations in D2R levels may be due to disruption of sleep in individuals with substance use disorders. Here we demonstrate that dopamine 2 receptor expression in the brain and the blood follows brain and blood dopamine levels on a timescale of minutes to hours following an acute dose of ethanol. This research provides evidence for transient changes in D2R expression following a single dose of ethanol.

Tonks, Adam; Domgaard, Hannah; Crowley, Valerie; Neumann, Gina; Keiser, Dylan; Metcalf, Josie; Guo, Hongjie; Zhou, Yi; Begemann, Mathew; Taylor, David; Jackson, Ryan (Utah State University)
Faculty Advisor: Jackson, Ryan (College of Science, Chemistry and Biochemistry)

Cms1 is a Type V endonuclease that contains a novel domain, shares little sequence homology with other Type V endonucleases, and in some organisms, is found near genes coding for other single-subunit nucleases. Studies in rice (Oryza sativa) have shown Cms1 capable of RNA-directed DNA editing. However, the mechanism of DNA cleavage remains unknown.
Here we present biochemical data that demonstrate Cms1 from Sulfuricurvum processes an RNA guide and binds/cleaves single- and double-stranded DNA through RuvC nuclease motifs. 2-D classification of structures obtained by negative staining electron microscopy show a major conformational change between SuCms1 bound and unbound to an RNA guide. The predicted global structure appears to be different than those reported for other Type V effectors. These data provide for a greater understanding of Type V endonucleases and may provide an alternative tool for genome editing applications.

Hutchison, Cortland; Preece, J. Caleb; Seibold, Tanner; Feland, J. Brent (Brigham Young University)
Faculty Advisor: Feland, Brent (Life Sciences, Exercise Science)

BACKGROUND: Muscle morphology changes with age and the fibrous/fatty infiltration should affect the overall stiffness of aging muscle. However, little info intrinsic stiffness as measured by SWE is available on aging muscle and the effect of gender using this measurement is not clear.
PURPOSE: This study aimed to determine how gender affects cross sectional area (CSA) and SWE of both the medial and lateral heads of the gastrocnemius muscle of the dominant leg in active older athletes participating in the HuntsmanWorld Senior Games.
METHODS: Data was collected from 116 volunteers (participants in the Huntsman World Senior Games) in St. George, Utah, 2019. Subjects (62 males: mean age = 68.9 ± 7.8 yrs, Ht = 177.4 ± 8.1 cm, Wt= 85.4±20 kg; 54 females: mean age = 66.9 ± 8.5 yrs, Ht =164.4 ± 7.2 cm, Wt= 69.8±18 kg) signed an approved consent form and then lay prone on a treatment table for ultrasonic measurement of both CSA and SWE of both heads of the gastrocnemius.
ANALYSIS: All data were analyzed a generalized linear model analysis using SPSS ver25 comparing CSA and SWE of both medial and lateral gastrocnemius heads by gender with age, height and weight as covariates.
RESULTS & CONCLUSION: We hypothesized that gender would affect both CSA and SWE with males exhibiting increases in both CSA and SWE values. We found a significant difference in CSA of both the medial and lateral gastroc (p=.000 for both) between genders, but no significant difference in lateral gastroc SWE (p=.337) or medial gastroc SWE (p=.320). Both age (p=.004) and weight (p=.000) were also found to significantly affect CSA and SWE between genders. The larger CSA values for men in this study may be more of a function of overall body size and weight since both populations are active athletes.

Tessem, Jeffery; Lloyd, Trevor; Brown, Nathan (Brigham Young University)
Faculty Advisor: Tessem, Jeffery (Brigham Young University; Nutrition, Dietetics and Food Science)

Diabetes is a global epidemic affecting millions of people. The total estimated cost of diabetes in the U.S. during 2017 was 327 billion dollars [1]. Diabetes is characterized by the loss of pancreatic β-cell function which is caused by an autoimmune disorder in Type 1 diabetes or insulin resistance and β-cell exhaustion in Type 2 (T2D) diabetes. Lifestyle changes in diet are beneficial in treating T2D. Phytochemicals are commonly utilized in these diets, and recent studies show diets high in flavanols exert beneficial bioactivity for β-cells. While flavanols demonstrate beneficial effects on β-cells, these flavanols are rarely observed in circulation, suggesting a necessary intermediate step. Flavanols are metabolized by gut bacteria to smaller metabolites that are absorbable. We hypothesize that these gut bacteria derived flavanol metabolites cross the gut and affect β-cell function. We have fed rats catechin supplemented or unsupplemented diets and collected urine as a means to isolate all absorbable gut flavanol metabolites. Here we present the effects of these absorbed metabolites on β-cell proliferation. This study begins to explain the mechanism by which flavanols exert their beneficial effect on glucose metabolism through the β-cell.

Hirschi-Forster, Jeanallie; Mendoza, Matthew; Van Oene; Nicholas ; Payton, Jullian (Weber State University)
Faculty Advisor: Clark, Daniel (Science, Microbiology)

The purpose of this research is to obtain quantitative data about possible sources for Salmonella contamination including tributaries to the Great Salt Lake, namely, the Jordan River, Weber River, and Bear River in Utah. We will also analyze specific water and soil sources near poultry farms for possible contamination. In recent studies, there is a greater number of produce items that have been found to contribute to Salmonella outbreaks. Contaminated water used for irrigation of these crops has been implicated as the causative agent for food contamination.

Bacteria found in these waterways are enriched using selective and differential media. This means, the media provides Salmonella species with required nutrients to grow effectively while differential media inhibits the growth of non-Salmonella species. The enrichment media that is used during this process is 3 X Tryptic Soy Broth and Gram-Negative broth. Gram negative broth is used as an enrichment step, but also selective in that it inhibits growth of other organisms. The two types of differential media would be XLT4 and MSRV. Salmonella is a motile bacterium and thus branches out from its original location of inoculation. This creates a halo-like growth pattern that makes it possible to differentiate Salmonella on MSRV plates. Once Salmonella is confirmed through the MSRV and XLT4 media, sequencing of its two CRISPR loci is completed. These two chromosomal regions have been shown to be distinct in different serovars, and as such, they can be used to distinct what subspecies is present in the sample.

Beales, Joseph; Lloyd, Trevor; Krueger, Emily; Barlow, Andrew (Brigham Young University)
Faculty Advisor: Tessem, Jeffery (Life Sciences; Nutritional, Dietetics, and Food Science)

Worldwide, an estimated 415 million people suffer from diabetes.1 Diabetes is characterized by chronic dysfunction of the pancreatic ß-cell, which leads to unregulated insulin secretion and abnormal blood glucose levels. Therefore, methods which increase the number of ß-cells or improve their function have potential for complementary treatment of type 2 diabetes. Compounds such as antioxidants and their gut metabolites have received attention in literature as having potential ß-cell-regulating properties.2,3 Therefore, we hypothesize that supplementation of grape seed extract (GSE), which is rich in antioxidants, will enhance ß-cell proliferation and insulin secretion. Accordingly, we obtained metabolites, derived from rats on either a control or grape seed extract diet, to measure the metabolites' impact on ß-cell function through in vitro assays such as glucose stimulated insulin secretion (GSIS) and 3H-thymidine incorporation. Discoveries regarding GSE metabolites' effects on ß-cell function could be fundamental to understanding ß-cell regulation and potential pharmaceutical or dietary treatments for diabetes.

1 Ogurtsova, K., et al. "IDF Diabetes Atlas: Global Estimates for the Prevalence of Diabetes for 2015 and 2040." Diabetes Research and Clinical Practice, Elsevier, 31 Mar. 2017, www.sciencedirect.com/science/article/pii/S0168822717303753?via%3Dihub.

2 Bajaj, Sarita, and Afreen Khan. "Antioxidants and diabetes." Indian journal of endocrinology and metabolism vol. 16,Suppl 2 (2012): S267-71. Doi:10.4103/2230-8210.104057

3 Tsuda, Takanori. "Recent Progress in Anti-Obesity and Anti-Diabetes Effect of Berries." MDPI, Multidisciplinary Digital Publishing Institute, 6 Apr. 2016, www.mdpi.com/2076-3921/5/2/13.

Walker, Carson; Burke, Tyler; Tanner, Call; Chaston, John (Brigham Young University)
Faculty Advisor: Chaston, John (Brigham Young University, Plant and Wildlife Sciences)

Host-microbe interactions can dramatically influence Drosophila melanogaster phenotypes, but few studies have explained how these microbes are recognized by the host. For example, fruit flies from one area, Maine, prefer to consume diets inoculated with Lactic Acid Bacteria (LABs) over diets containing Acetic acid bacteria (AABs); whereas flies from another area, Florida, show no preference for either LAB or AAB. However, the bacterial mechanisms responsible for this preference are unknown. My follow-up analyses further suggest the hypothesis that Maine flies avoid AAB, rather than are attracted to LAB. Therefore, I propose a forward genetic approach to define the AAB processes that shape this fly preference. I will do this by comparing the feeding preferences of Maine and Florida flies to diets incorporated with LAB versus specific bacterial molecules, such as acetic acid, peptidoglycan, and lipopolysaccharides. Alternatively, if these molecules do not mediate the effects I will perform a metagenome wide association assay (MGWA) to identify genes linked to this preference. Together, these approaches will help to reveal the bacterial factors that influence fly feeding preferences.

Stoneham, Lisa; Brasso, Dr. Rebecka (Weber State University)
Faculty Advisor: Brasso, Rebecka (Weber State University, Zoology)

Mercury is a toxic heavy metal that poses significant health threats to people and wildlife. The organic form of mercury, methylmercury, is converted from its inorganic form via microbial methylation. Methylmercury is dangerous because it attaches to proteins in the blood, muscle, and other tissues and can cross the blood-brain and placental barriers. Microbial methylation is enhanced in anoxic environments such as wetlands, which are increasingly being classified as mercury hotspots where animals accumulate elevated concentrations relative to those in terrestrial systems. This is concerning for the wetlands of the Great Salt Lake due to its history of anthropogenic inputs of pollutants and its importance as a breeding ground and rest stop for migrating avian species. Previous research has shown significant mercury methylation occurring within the Deep Brine Layer of the GSL. The purpose of this project was to investigate potential spatial variation in mercury concentration in different portions of the GSL. With a focus on invertebrates, we collected insects including brine flies, midges, damselflies, and crane flies from three sites of varying salinity around Antelope Island State Park: Farmington Bay, White Rock Bay, and the Antelope Island Marina. Mercury concentrations in insects were determined using a Nippon MA-3000 Direct Mercury Analyzer. Our results will provide a preliminary assessment of mercury concentrations in flying insects from different habitats around the island. This will help in determining differential risk to insectivorous songbirds, waterfowl, and shorebirds foraging on these common prey species in the GSL.

Jensen, Daelin; Baxter, Melanie (Brigham Young University)
Faculty Advisor: Tessem, Jeffery (Nutrition, Dietetics, and Food Science; Life Sciences)

Approximately 1.25 million people are currently living with type 1 diabetes. By 2050, 5 million people are expected to be diagnosed with the disease1. The insulin secreting pancreatic beta cells are essential to control proper glucose absorption and storage in insulin sensitive peripheral tissue. Both type 1 and type 2 diabetes are characterized by decreased functional beta cell mass and, consequently, decreased insulin production. One potential intervention is the use of beta cell transplantation from cadaveric donors. A major impediment to greater application of this treatment is the scarcity of transplant ready beta cells. Increasing the quantity of functional beta cells for transplantation will lead to increased insulin production and better management of the disease. Various genes have been defined that can induce beta cell replication. A major caveat of these findings, however, is that these factors induce replication in young beta cells but not in aged beta cells. Age-dependent morphological changes in the beta cell are poorly understood, despite its relevance to type 1 diabetes: here, we show that insulin-positive tissue area changes with age. Given that the majority of beta cells that will be used for transplant will come from aged donors, it is imperative to understand why aged beta cells are refractory to the aforementioned proliferative mechanisms. The cell cycle is tightly regulated by cyclin-dependent kinases. Cyclin-dependent kinase inhibitors (CDKI's) bind to cyclin dependent kinases, inhibiting cell proliferation. We hypothesized that these CDKIs are responsible for the observed lack of proliferation in aged animals. We demonstrate the expression of the Ink4 and Cip/Kip family of CDKI's by mRNA, protein and histological expression in 5 week and 5 month old primary rat beta cells. In addition, we show how size-related expression differences of CDKIs relate to beta cell proliferation.

Hannah A. Veltkamp, Sydney Houghton, Michael T. Stevens (Utah Valley University)
Faculty Advisor: Stevens, Micheal (Utah Valley University, Biology)

Netleaf hackberry (Celtis reticulata) is a deciduous shrub native to the southwestern United States and northern Mexico. Individual shrubs can be long-lived, but newly established stands of hackberry are rare. The lack of juvenile hackberry in the wild could be due to low germination rates reported in both laboratory and field settings. The seeds of hackberry are embedded in drupes that are an important source of food for birds and small mammals. Animals likely play an important role in seed dispersal, and passing through a digestive tract could increase the germination rates of hackberry seeds. Passage through the digestive tract of a mammal can increase the germination rates for some plant species, but not for others. We hypothesized that passage through the digestive tract of a coyote would increase the germination rates of C. reticulata. To test this hypothesis, we collected 17 coyote scats containing visible hackberry fruits from along the Bonneville Shoreline Trail east of Provo, Utah, using latex gloves. Each scat location was recorded using a GPS unit. After collecting each scat, we found the closest hackberry shrub and picked a sample of fresh hackberry fruits from it. All samples were cleaned and cold stratified and then planted into cone-tainers containing a potting soil mix and placed in the Utah Valley University greenhouse. We sowed 20 seeds from each of the 17 coyote scats and

20 seeds from each of the neighboring hackberry bushes for a total of 680 seeds. The 680 cone-tainers were labeled with plastic stakes and randomly positioned into trays that were randomly distributed on a bench in the greenhouse. The seeds, and later seedlings, were watered as needed (typically three days/week). On watering days, we checked for newly-germinated hackberry seedlings and recorded their date of emergence. Near the end of the experiment, we measured the height of each seedling. The germination rate of hackberry seeds that had passed through the

digestive tract of a coyote did not differ from the germination rate of seeds from fresh-picked fruit (42.7% vs. 46.5%, respectively; _ 2 = 0.558, df = 1, p = 0.455). However, on average, the coyote-treatment seeds took just over half as many days to germinate as did the seeds from fresh-picked (undigested) fruit (35 days vs. 69 days, respectively; p < 0.001). The seedlings from coyote-treatment seeds were 9.5% taller than were the seedlings derived from seeds from undigested fruit (6.4 cm vs. 5.8 cm, respectively; p < 0.001). Our results show that consumption by coyotes can benefit hackberries by enabling their seeds to germinate earlier in the year when

conditions for establishment are good. The earlier start on germination that coyote-ingested hackberries get translates to increased height and likely a higher rate of survival in the field.

Elison, Weston; Bauchle, Casey; Bunker, Libby; Stephens, Samuel; Tessem, Jeffery (Brigham Young University)
Faculty Advisor: Tessem, Jeffery (Brigham Young University; Nutrition, Dietetics and Food Science)

Type 2 Diabetes (T2D) effects hundreds of millions of people worldwide, with that number increasing rapidly. It is characterized by increased insulin resistance and dysfunctional insulin secretion. The beta cell of the pancreas is the primary insulin secreting tissue, found in the endocrine tissue of the pancreas called islets of Langerhans. In T2D beta cells become glucose intolerant and disease progression is characterized by loss of functional beta cell mass. Previous studies have shown that the transcription factor Nkx6.1 is vital for beta cell differentiation, identity, and insulin secretion. Research has indicated that Nkx6.1 expression and protein levels decrease in pancreatic islets from human donors with T2D. Our data indicates that glucolipotoxicity, a common model for obesity and diabetes in cell culture, leads to decreased Nkx6.1 mRNA expression, protein levels and nuclear localization in Ins-1 832/13 cells. Nkx6.1 regulates genes in the nucleus , and its loss inhibits proper insulin secretion. We propose that reactive oxygen species created by metabolism of excess fuel decreases Nkx6.1 expression and Nkx6.1 target gene expression, as measured by quantitative polymerase chain reaction (qPCR). Also, increased glucose concentrations causes increased Nkx6.1 protein degradation and translocation out of the nucleus. Protein levels will be measured by western blot and localization by confocal microscopy. In order to understand how these changes effect beta cell function, we will measure glucose stimulated insulin secretion by sandwich Enzyme Linked Immunosorbent Assay (ELISA). We further propose that Nkx6.1 overexpression will restore beta cell function. These results will assist in unraveling the cause of beta cell dysfunction in T2D.

Lidgard, Audrey; French, Susannah; Hudson, Spencer (Utah State University)
Faculty Advisor: Lidgard, Susannah (College of Science, Biology Department)

Ectothermic organisms, such as reptiles, rely on the external environment for regulating internal temperatures necessary for vital physiological processes. When faced with environmental challenges, temperature may differentially affect how allostatic mediators (e.g., glucocorticoid hormones) are released to mediate energy allocation for handling stressors. Subsequent differences in energy mobilization and circulating metabolites during a stress response may ultimately influence self-maintenance processes such as immunity. The aims of this research were to determine how stress sensitivity varies with diurnal temperatures in the Plateau Side-blotched Lizard (Uta stansburiana uniformis) and to assess the potential implications for immune function. Both baseline and stress-induced levels of glucocorticoids (corticosterone) and energy metabolites (glucose) were compared to body temperature and the thermal environment. Variation in innate immune function (bactericidal ability) was then compared to both temperature and physiological parameters at baseline and stress-induced levels. Stress reactivity via glucocorticoid release positively corresponded with body and environmental temperatures, although glucose release did not. Bactericidal ability subsequent to a stressor negatively corresponded with body temperature and glucocorticoid release. Such findings provide further insight on how stress sensitivity and self-maintenance can vary across the thermal environment, posing potential fitness consequences for an ectothermic organism.

Karin, Kettenring; Robison, Talin; Leonard, Emily (Utah State University)
Faculty Advisor: Kettering, Karin (S.J. & Jessie E. Quinney College of Natural Resource, Watershed Sciences Department)

The Great Salt Lake (GSL) and its wetlands are important habitat for migrating birds. The GSL wetlands provide crucial habitat for nesting, food, and areas to recover from migration. Common carp are a threat to GSL wetlands. Carp disturb sediments in the water, blocking some of the sunlight from entering the water, which is utilized by aquatic macrophytes and algae. Carp also may be affecting invertebrate populations, which are critical food resources for migrating birds, but these effects have not been well-documented. My research addressed the question: what are the effects of invasive common carp on invertebrate food sources for diving ducks in the Great Salt Lake wetlands? I answered my research question by addressing the following objectives: (1) to identify the benthic, epiphytic, and water-column dwelling invertebrates in Farmington Bay Waterfowl Management Area (WMA), and (2) to determine if common carp are having an impact on the overall density, diversity, and abundance of the invertebrate communities fed on by diving ducks. I compared invertebrate communities (diversity and abundance) between carp-excluded boxes and control boxes. I constructed my carp exclosures of wire mesh and t-posts to prohibit carp from entering while still allowing invertebrates and water to freely move in and out of the exclosure. The control boxes were constructed of t-posts and allowed carp to freely enter and exit the box. I used dipnet and substrate core samples to determine what invertebrates are living in the water column and substrates at Farmington Bay wetlands. Although sample processing is on-going, early results indicate that carp reduce water column invertebrate abundance while effects on invertebrate diversity are thus far inconclusive. Given the importance of GSL wetlands and their invertebrate food sources to migrating diving, my research findings underscore the importance of aggressive carp management.

Gottfredson, Sarah; Chaston, John (Brigham Young University)
Faculty Advisor: Chaston, John (Life Sciences, Plant and Wildlife Sciences)

The microbiome of Drosophila melanogaster can have significant effects on the host, and many of these have been studied. However, the reason why the bacterial species associate with and persist in D. melanogaster has not been studied in depth. Here we define persistence as how long a microbe associates with a host. The early assumption has been that the D. melanogaster gut microbiome is established solely through diet, but recent work suggests that other factors may be at play in the microbiome establishment. This experiment aims to study the correlation between bacterial genotype and persistence in the D. melanogaster microbiome. In this study, a metagenome wide association (MGWAS) was done using 40 different strains of bacteria to find distinct bacterial genes that are significantly correlated with persistence. To do this, each strain was mono-associated with twenty-four individual flies. The flies were reared for fourteen days, transferred onto new food three times a day for two days, homogenized, and plated. Using the significant genes found through the MGWAS, the same experiment protocol will be used to test mutants of these genes for their effect on persistence. These data will provide us with distinct genes that are necessary for effective bacterial persistence.

Not yet published (Brigham Young University)
Faculty Advisor: Chaston, John (Brigham young University, Life Sciences)

Within an organism's gut are many strains of bacteria that are constantly interacting with their host. Microbiota composition has been shown to impact many aspects of host health such as metabolism, fat-storage, starvation resistance, and reproduction. Certain behaviors and outcomes have been correlated with certain microbial taxa present in the host gut.

D. melanogaster serves as a useful tool for studying this relationship because its microbiota contains relatively few bacterial strains and is both widely studied and largely understood. Previous research within our lab involving D. melanogaster has found trends in many life-history strategies (ie. reproduction, fecundity, lifespan) that correlate with the presence of certain gut bacteria. While there are many aspects of health that microbiota composition affects, there are also a variety of factors that impact microbiota composition thus leading to these end results.

This experiment seeks to further understand the role that environment has in determining microbiota composition. By rearing gnotobiotic flies in environments that differ in temperature, we can then analyze microbiota content to see if any fluctuations occur due to environmental temperature. If temperature is found to have an effect on the taxa present in fully developed D. melanogaster, we can then seek to determine whether or not there are evolutions taking place in host genotype that yield differing microbiota phenotypically.

Thornton, Sherie; Cardona,Rebecca (Weber State University)
Faculty Advisor: Culumber, Michele (Weber State University, Microbiology); Oberg, Craig (Weber State University, Microbiology)

Lactobacillus wasatchensis was initially isolated from cheese produced at Utah State University and was found to be a Non-Starter Lactic Acid Bacteria (NSLAB) that causes late-gas production in cheese that can damage packaging and produce defects in the cheese. The goal of this project was to locate an environmental reservoir for Lactobacillus wasatchensis. Five samples of silage that were in different stages of fermentation and content and raw milk samples were obtained at the Utah State University dairy. Samples were serially diluted, plated on de Man, Rogosa and Sharpe agar supplemented with 1% D-Ribose (NRS-R) and incubated anaerobically for 5 days. Colonies that looked like potential Lb. wasatchensis were selected and regrown for isolation. All isolates were gram-positive rods. The isolates were further grown in broth for DNA extraction, sequencing, and analysis with API 50 carbohydrate panel (API 50CH). The API 50CH results were significantly different from Lb. wasatchensis, which only demonstrates use of ribose in this assay. Sequencing of the 16S rRNA gene, however, produced a match to three isolates from two different silage samples that had 99% sequence identity to Lb. wasatchensis. Further analysis of the isolates is being done to confirm this finding and describe the organism isolated from the soil. We hypothesize that these organisms are very closely related to Lb. wasatchensis and that silage could be an environmental source of contamination.