Physical Sciences
Symmetric Electrochemical Cells in Nonaqueous Organic Redox Flow Batteries – Applications for Longevity Study
Presenter: Julia Case, College of Science, Chemistry
Pursuit of Methods for Antibiotic Synthesis: The Synthesis and Formal [2+2] Cycloaddition Reactions of 1-Phenylprop-2-en-1-one
Presenter: Madison Fisher, College of Science and Engineering, Physical Science
Bioconjugation of Rhodamine-B and C-Phycocyanin from Spirulina Sp.
Presenter: Tyler Holmes, College of Science, Chemistry
Engineering a microbial fuel cell to reduce methane production in waste water
Presenter: Jessica Hertig, College of Sciences, Physical Science
Elucidating the Molecular Mechanism of Action of the Microsclerodermins
Presenter: Billy Nguyen, College of Science, Chemistry
The Effects of Pterostilbene on the Oxidant Status of Red Blood Cells in Hyperglycemic Conditions
Presenter: Hana Hanks, College of Physical Sciences, Chemistry
Laser Mediated Photochemistry: Exploring the Isomerization and Decaying Behavior of Dipyrroles
Presenter: Taime Clark, College of Science and Engineering, Physical Science
Measuring Aluminum Fluoride Optical Constants in the EUV
Presenter: Devin Lewis, College of Physical and Mathematical Sciences, Physics and Astronomy
The Effect of Roughness on Blazed Gratings in the EUV
Presenter: Ethan Edwards, College of Physical and Mathematical Sciences, Physics and Astronomy
Phylogenetic Relationships of the Flat-headed Mayflies (Ephemeroptera)
Presenter: Taylor Murphy, College of Science, Biology
TELSAM-target protein fusions can form diffraction-quality crystals without direct inter-polymer contacts
Presenter: Braydan Bezzant, Brigham Young University, Chemistry and Biochemistry
Preparation of Monoalkyldiphenylphosphonate by use of Grignard Reagents and Lewis Acids from Triphenyl Phosphate.
Presenter: Sydney Rowley, College of Physical Science, Chemistry
Thermal Imaging of Groundwater Sources into Utah Lake, UT, USA
Presenter: Allison Smith, College of Science, Earth Science
Determination of the Relationship Between Hypertension and Endogenous Ouabain Placental Concentration in Preeclampsia
Presenters: Samantha Tilley, College of Science, Chemistry
Analyzing Fatal Bird-Window Collision Mitigation Occurring at the Classroom and Student Services Building, Brigham City, UT.
Presenters: Hunter Martin, College of Science, Ecology
Investigations of force dependence in an atmospheric negative corona discharge between two parallel wires on electrode asymmetry
The general purpose of this research is to investigate the force produced in a negative corona discharge between two parallel wires at atmospheric pressure. This phenomenon has been applied to air purification, cooling of electronics, and optimistically theorized as means for the primary mode of propulsion for small aerial drones. Previous work has elucidated a relationship between force and current, spacing of electrodes, and applied potential. This research specifically deals with quantifying the strength of the relationship between force and asymmetry due to differing electrode radii. The force measurement is obtained via a difference in weight of the hanging parallel wire assembly and many different radii of anode and cathode will be studied. Current results will be discussed.
Impact of Micro- and Nano-Structured Surfaces on Condensation Heat Transfer Effectiveness
Every year, millions of dollars are spent to cool systems that rely on evaporation and condensation of water, such as power generators and desalination plants. Historically, cooling methods have utilized heat exchangers to cool and condense water vapor to be used in a power cycle or for drinking water. This has been found to be somewhat ineffective due to the filmwise type of condensation that occurs, where the surface tension of water does not readily allow for new droplets to form on the heat exchanger surfaces. Superhydrophobic micro- and nano-structured surfaces have recently been discovered to promote high-mobility drops in dropwise condensation, which greatly increases a surface’s ability to cool and condense water vapor. Dropwise condensation is much more effective because droplets can more easily slide off to make room for new nucleation sites, which leads to increased rates of heat transfer. Some of these droplets combine as they slide to clear off even more surface space, and droplets can even spontaneously jump away from the surface as they coalesce due to imbalances with surface tension. Despite the significant academic attention these surfaces have received in recent years, relatively little research has been conducted on the overall effectiveness of these superhydrophobic surfaces in condensation heat transfer. Another important question that lacks addressing is how to improve their survivability, since the surfaces tend to be damaged and rendered unusable easily. The objective of this project is to explore the effect that superhydrophobic surfaces have on condensation heat transfer and to identify factors that lead to sturdier, more conductive surfaces.
MultiCRAFTI: Overcoming the Kinetic Energy Limitation to Measure the Cross Section of High-Mass Ions
CRAFTI is an emerging method for measuring molecular collision cross sections using the ultra-high resolving FTICR-MS instrument. Collision cross sections are important measurements to understanding size, tertiary structure, and ligand binding. CRAFTI has been shown to give accurate collision cross sections comparable to those from drift ion mobility, the leading cross section measurement method. Because CRAFTI measurements are carried out under single-collision conditions, CRAFTI can obtain cross sections for non-covalently bound complexes with interactions that are far too weak to address using drift ion mobility, where multiple thermal collisions can disrupt the complexes. However, CRAFTI’s requirement of single-collision ion dephasing is a hindrance to the measurement of larger mass ions because for ions that are massive compared to the neutral collision partner it is difficult to reach sufficient center-of-mass kinetic energy to cause dephasing (which usually occurs via collision-induced dissociation). In this study, we characterize these limitations by measuring different species (tetraalkylammonium, cryptand-metal ion, and cucurbituril complexes) at a range of kinetic energies. For polyatomic ions, CRAFTI cross sections are observed to increase with increasing center-of-mass kinetic energy until they reach a limiting value that is generally similar to cross sections computed from the expected molecular structure. The CRAFTI measurement reaches the calculated cross-section at a specific center-of-mass kinetic energy related to the energy of the bond being broken. We also performed MultiCRAFTI, a new experiment where we overcome the kinetic energy limit through simultaneous measurements of multiple species for internal comparison. Two analytes in a MultiCRAFTI experiment, though both are beneath the minimum kinetic energy requirement, give correct relative cross sections. These MultiCRAFTI measurements represent a new step toward extending the FTICR-MS to vital new areas of measurement in macromolecules and organo-metal complexes.
Osseointegration on a Carbon-Infiltrated Carbon Nanotube Coated Titanium Surface
Osseointegration plays a vital role in orthopedic surgery, as bone cells should be able to adhere and form on the implant surface to enable strong mechanical connection. Titanium has long been used in orthopedic and prosthetic implants because of its known toughness, strength and low conductivity, and especially its biocompatibility. However, native bone growth onto titanium is not optimal. Previous work in our lab has developed a carbon-infiltrated carbon nanotube surface which exhibits structural antimicrobial properties. The purpose of the present work was to evaluate osteoblast growth on this surface to determine whether the coating was cytotoxic, neutral, or osteogenic. Vertically aligned carbon nanotubes were grown on top of commercially pure titanium samples using ethylene and hydrogen gas at 750C, followed by infiltration at 900C. Our control sample was an uncoated commercially pure titanium sample. Both the control and CI-CNT coated titanium samples were cell cultured with human osteoblasts kept at 39C for approximately 96 hours. The preliminary tests have shown that the CI-CNT coated titanium sample promotes enhanced osteoblast proliferation, as well as functionalization (e.g., they are laying down calcium as part of bone formation). These results may lead to an orthopedic implant coating which provides both structural antimicrobial behavior, as well as enhanced osseointegration.
Resistivity of Nickel Thin Films
As metal films get thinner, their resistivity increases. We study the phenomenon using nickel films. The nickel films are made in a vacuum system with pressures below 10^-7 Torr. A nickel filament is heated to 1200°C to sublimate the nickel onto our glass substrate. A four-point measuring probe was used to measure voltage drops across the film at known currents. This gave a sheet resistance. Thicknesses of the thin films was verified by images on a scanning electron microscope. Thicknesses varied from 40nm-255nm. Resistivity of films were calculated to be between 22-147Ωnm. A graph of resistivity vs thickness will be presented and discussed on this poster.
Uncertainty in Optical Particulate Counting Sensors
In order to mitigate the health problems and environmental damage caused by the burning of biomass in homes across the developing world, there is an international effort to design clean burning cookstoves with greater efficiency and fewer harmful emissions. An important measuring tool for gauging the effectiveness of these alternate stoves is the optical particulate counter. This type of sensor beams light through the contaminated air and measure how much of that light is refracted. Based on these measurements, the size and amount of particles in the air can be inferred. However, for the most part, these measurements are taken at face value, without an understanding of the amount of uncertainty involved in the measurement process. This project describes the development of an accurate mathematical model for a particular sensor, the OPC-N2 by Alphasense, and states how this model leads to increased understanding of the uncertainty involved in the operation of our sensor and therefore, sensors like it.
Jet Impingement on Superhydrophobic Surfaces: Effect of Post Microstructure on Heat Transfer
Liquid jet impingement is an excellent solution for many thermal management challenges (e.g. cooling of advanced microelectronic devices) because of its high heat transfer rates. Superhydrophobic surfaces, with micrometer scale posts, have desirable self-cleaning and anti-fouling properties and their use has been proposed in these applications; however, superhydrophobic surfaces also decrease heat transfer rates. The relationship between superhydrophobic surface post microstructure and heat transfer rate in liquid impinging jets has not yet been explored. To better understand this relationship, experiments are conducted with varying pitch, the distance between posts, and varying cavity fraction, the area not covered by the posts divided by the total area. This empirical data is then utilized to develop models, which will predict heat transfer based on pitch and cavity fraction. Designers of thermal management systems may then install post patterned superhydrophobic surfaces in thermal management devices to utilize their desirable properties and may maximize heat transfer rates by selecting the optimal pitch and cavity fraction.
Public Understanding, Opinions, and Questions about Hot Topics in Science
Past research has shown that, though the U.S. public generally views science favorably, the benefit of scientific inquiry has decreased in value. According to the PEW Research Center (2015), only 79% of people thought science was generally positive and made life easier for most people. This paper seeks to explore why people may view science negatively by gauging participants’ responses to genetically modified organisms, a controversial topic in science. Participants were gathered from upper division writing courses at the University of Utah, and they were asked to complete a science knowledge quiz, several questionnaires, an interview, and a think aloud protocol. Their responses were analyzed to determine their science knowledge and what questions they had about GMOs and science. Preliminary findings show most questions were geared towards the methods behind GMOs. References: Funk, C.,Rainie, L., and Page, D. (2015). Public and scientists’ views on science and society. Pew Research Center. Retrieved from http://assets.pewresearch.org/wp-content/uploads/sites/14/2015/01/PI_ScienceandSociety_Report_012915.pdf
Habitability of Martian Recurring Slope Lineae: Building Capacity for Exploring Terrestrial Analogs for Astrobiology
Recurrent Slope Lineae (RSL) are dark, narrow streaks of flowing brine water on Martian hillsides. Perchlorate salts found in especially high abundances in RSL regions may be responsible for their formation. It is believed that through a process called deliquescence, the salts act as a type of “sponge,” absorbing atmospheric water and seasonally saturating the hillside. These saturated soils may act as a niche for life. In order to better understand the potential habitable space of RSL, we created RSL analogs with various salt and moisture concentrations. The analogs were then impregnated with extremophilic microbial communities derived from Beacon Valley, Antarctica. Metagenomic and metatranscriptomic results assess the potential habitable space of RSL on Mars.
Blu-ray Thermal Scanning Microscope
The purpose of this research is to develop a non-contact method for measuring the thermal properties of a material. The technology used is fluorescence thermometry, which involves reading the fluorescent signal emitted by a material and relating it to temperature. Although this is not a new technology, this application of the technology will allow us to measure properties at a microscopic level. To achieve this, we are using the PHR-803t laser optics found in the discontinued XBOX 360 HDDVD reader. This device was chosen due to its low cost, as well as the availability of an open-source project that provided a basis for controlling the laser optics. The temperature capture process is as follows: coat a sample with a photo-sensitive material; expose sample to blue laser (405 nm); heat the sample by modulated infrared laser; capture the emitted fluorescent signal with a photodiode; obtain the magnitude and phase shift of the fluorescent temperature compared to the heating laser through lock-in amplification; calculate temperature based on the magnitude and phase shift; fit theoretical curve to determine thermal property. My contributions to this research have been in the control of the lens actuator and laser diodes contained in the PHR-803t, lock-in amplifier circuitry, control of actuators for translation of the sample in 2D space, and system integration and design.
A fossil-pollen-based climate reconstruction of two lake records from the Uinta mountain range in northern Utah.
Climate change is an issue that has gained a significant amount of attention in recent years. Specifically, anthropogenic climate change, as we know the Earth's climate goes through cycles of warming and cooling. In recent years, there have been examples climate changing rapidly and creating problems for communities around the world. The goal of this study is to look at patterns of climate change through reconstructing a climate history by analyzing two frozen lake sediment cores northern Utah. The two study sites are from both Newt Lake outside of Vernal Utah and Canyon Lake located outside of Duchesne Utah. In this study we have counted the charcoal from the sediment cores, and we have analyzed the pollen grains in the core. As we count the charcoal from the frozen lake sediment core we are able to determine the study site's fire interval and look at charcoal peaks this can help establish a general idea of how often fires come through the two study sites. Once we gather this data we can examine it to determine if there has been any changes in the fire interval in recent years. Additionally, as we prep and analyze the pollen data in the sediment core we can look at the individual species of pollen to determine the climate envelope of each species to get some idea of what the climate would have looked like at a given time. This study will provide the reader with information about how climate has been changing in Utah and give insight as to what the study sites may have looked like at a given time compared to today.
Sources and Composition of Atmospheric Particulate Matter in the Salt Lake Valley
Air pollutants pose a significant health risk in urban environments. Particulate matter 2.5 microns or smaller (PM 2.5) is of special concern because it can penetrate deep into lungs. Salt Lake City (SLC) is well-known for its winter inversions with PM 2.5 levels frequently exceeding EPA standards, but air pollution during the summer months is of growing concern. While dust storms are known to occur each year in SLC, the predominant source of the atmospheric particles, their origin, chemical composition, and particle size distribution have yet to be identified due to lack of research. Newly exposed lake sediments are highly prone to dust generation. The recent drought in 2011-2016 caused a 30% decrease in the volume of the Great Salt Lake (GSL), and water levels in the GSL fell dramatically to record lows in 2016, exposing large tracts of dry lake bed. It is unclear to what extent these exposed GSL sediments produce dust, and if that dust affects air quality in the Salt Lake valley or represents an important source of atmospherically transported metals to downwind aquatic environments. Our study was designed to answer these research questions. Samples of total particulate matter and PM 2.5 were collected with atmospheric samplers at Westminster College and several other sites. Samples were then digested and analyzed for their trace element and heavy metal content by ICP-MS. Though this study is ongoing, preliminary results suggest a possible increase in particulate matter as the summer progresses, potentially associated with periods with elevated atmospheric particulate matter in the Salt Lake Valley, in part derived from wildfires in the wider region.
Grain Boundaries and Diffusivity
Diffusion of atoms through materials is important for a number of material processes, such as air purification, fuel cells, and material behavior. The interfaces between crystals, also known as grain boundaries, are considered to be rapid diffusion pathways through a material. To better understand how these grain boundaries affect diffusion in a material, we simulate diffusion in a variety of atomic structures. The goal is to correlate the diffusivity with other properties of the grain boundaries, such as their energy.
Using Exoplanet Transits to Calibrate the Great Basin Observatory
Southern Utah University is one of four university partners who manage and operate the new Great Basin Observatory (GBO). As partners, SUU students have priority access to the remotely operated telescope for astronomical research. Since the GBO telescope is new, its capabilities are still unknown. In order to determine the telescope’s limits, we have started taking photometric measurements of exoplanet transit systems. We then verify that the photometric data is consistent with accepted values. So far we have successfully processed several transits. We plan to continue detecting exoplanet transit systems with lower signal to noise ratios until the transit dimming is too faint for our telescope to detect. Once our work is finished, we will have a better understanding of the GBO limits which will benefit future SUU students and other GBO university partners.
Design of Martian Recurrent Slope Lineae Analogs to Study a Potential Environmental Niche for Life
Until 2011, researchers knew of only two scenarios where life could exist on Mars: underwater aquifers that were remnants of ancient Martian seas and melting ice at the Martian poles. Yet, evidence now strongly suggests additionally the presence of liquid water on Mars through a phenomenon called reucurrent slope lineae (RSL). Through the action of deliquescence of hydroscopic salts such as magnesium perchlorate, water is likely collected from katabatic winds, forming salty brines on the surface of Mars. RSL sites are found predominantly in the Martian equatorial region. Because RSL provide a setting for liquid water to exist, they may serve as a habitable niche on Mars. To assess this scenario, we independently created RSL analogs and impregnated them with Antarctic microbial extremophiles. The design of our RSL analogs is based on a suite of water concentrations and magnesium perchlorate concentrations, allowing us to represent the variety of RSL geochemical compositions that could be possible on Mars. Since RSL analogs are based a Martian Mojave simulant that is both autoclaved and baked at 250 -, we minimized the possibility of bacterial or amino acid contamination. Current and future studies on RSL will be enhanced based on the foundation that our project provides.
Synthesis and Characterization of Low Toxicity Extraction Solvents
It cannot be refuted that water is a precious resource, and society faces a challenge as the demand for clean water sources increases. Presence of heavy metal contaminants and other toxic organic compounds render water useless for consumption without going through extensive processing. However, some of the metal contaminants have market value; therefore, efficient extraction methods which support recovery of these precious metals are essential. Hydrophobic materials that have high affinity for target metals can sever as effective solvents in the biphasic extraction process. Ionic liquids, which are organic salts that exist as liquids at room temperature, can be designed to possess hydrophobic properties and can function as eco-friendly means of separating rare earth heavy metals. Additionally, the ionic liquids can also be used to extract and recover organic compounds such as biofuels. The focus of this research is the synthesis and characterization of phosphonium based ionic liquids, designed to exhibit high extraction efficiency of metals and organic compounds while exhibiting low toxicity to organisms. The ionic liquids were tested for extraction of various organic compounds, including biofuels, as well as for metal complexes. This new approach to extraction would support an environmentally friendly method of purifying contaminated water systems and for the extraction and recovery of commodity chemicals.
Unexpected Copper Nanoparticle Synthesis from Bulk Copper Metal
Copper nanoparticles have applications in many fields including antibiotics and engineering nanofluids. The difficulty with producing copper nanoparticles, compared to synthesizing gold or silver nanoparticles, is that copper nanoparticles oxidize when exposed to oxygen. Oxidation causes nanoparticles to dissolve. We have discovered that hydrochloric acid dissolves copper metal when sonicated. This is a result not expected thermodynamically. Likewise, at low hydrochloric acid concentrations copper nanoparticles form when the solution is sonicated. Neither of these results are expected and neither have been used to explore methods for copper nanoparticle synthesis. Using these findings, we developed a new method to synthesize copper nanoparticles from bulk copper metal. We are testing how controlling the level of oxygen effects the nanoparticles produced. We are working to find optimal oxygen concentration as well as hydrochloric acid concentration to consistently synthesize nanoparticles. We will report on our results up to this point.
Effects of fire on aquatic ecosystems
The Brian Head fire, which began due to human action in June 2017, quickly wreaked havoc in the western corner of the Dixie National forest. It covered roughly 72,000 acres of land1. The full impact of the fire has yet to be discovered, but there is an urgent necessity to evaluate the drastic changes to the ecosystem. In this study, we aim to look into variations in water chemistry of two streams crossing the Brian Head burn scar. We will conduct weekly monitoring of the pH, dissolved oxygen, water velocity, temperature, and turbidity. Our two sites are located on along the Second Left Hand Road, in Middle Creek and Parowan Creek. Our goal is to evaluate how the fire affects these streams not only through ash deposits but flooding and erosion as well. Water velocity and turbidity will be more reflective of punctual episodes of flash flooding that have occurred on multiple occasions in the Parowan area since the fire. We can see that the road and the stream banks have already been significantly altered, our study will allow us to have a deeper understanding of how the fire affected the area and its ecosystem. Future studies will include and biological assessment through the invertebrate community of the streams. 1 (2017, June 18). Brian Head Fire. InciWeb. Retrieved from www.inciweb.nwcg.gov
Nanoparticle Synthesis with Microfluidic Devices
Our research focuses on the synthesis of silver nanoparticles using microfluidic devices. These devices are of particular interest to us because of the controlled environment that they provide for reactions to take place. As the name suggests, the “micro” scale of this device gives us a high surface area to volume ratio while the “fluidic” aspect allows a controlled flow rate and pattern. This provides reproducible products with accuracy and precision as we control exactly when turbulent and laminar flow occurs. Our microfluidic device is created by using Polydimethylsiloxane (PDMS), a polymer that is similar to glass. Using a magnesium wire we can shape our microfluidic device to fit the needs of our experiment and then suspend it in PDMS. The magnesium wire can be dissolved once the PDMS solidifies, forming a hollow chamber in which we can perform our reaction and synthesize our silver nanoparticles. Silver nanoparticles have a wide range of applications but the focus of our research will be to learn more about what causes inconsistency in the size of the particles. Since one of the major challenges of nanoparticle synthesis is creating a product that is uniform in size, learning how to control the synthesis process to create highly uniform nanoparticles would be beneficial. To accomplish this, we plan to gather more information about the nanoparticles using Raman spectroscopy. We will also use a UV-Vis spectrometer and spectrofluorimeter to analyze the stages of nanoparticles synthesis.
Geophysical Survey of Gossans in the Eastern Uinta Mountains, Utah
When sulfide-bearing rocks are exposed to oxidizing conditions, they become destabilized, leaving behind a framework of leached, altered, and replaced host rock called a gossan. Many of these gossans form by the oxidation of ore minerals and have been known since antiquity to be associated with ore deposits. However, the extent and quality of ore mineralization beneath a given gossan cannot readily be determined through surface sampling of minerals. Work conducted by mineral exploration professionals (in Africa, Australia, India, and the Middle East) has indicated that geomagnetic and geoelectric surveys of a gossan can be useful for constraining the shape, size, and economic potential of an associated ore deposit. Although gossans are found in Utah, there have been no published studies of these rock units either in terms of their economic potential or geophysical signature. The objective of this study was to carry out geomagnetic and geoelectric surveys to determine the geophysical signature of gossans exposed about 10 miles northwest of Vernal, Utah, on the southeastern margin of the Uinta Mountains, for the purpose of estimating the grade and depth of possible sulfide mineralization. The gossans overlie a heavily brecciated wedge of Mississippian Madison Limestone and are structurally bounded by the South Flank Fault, which forms the boundary between the Permian Weber Sandstone to the south and the Neoproterozoic Uinta Mountain Group metaquartzites to the north. Geoelectric measurements with an Iris Instruments Syscal Junior Resistivity System and inversion of a portion of the resistivity and chargeability data with the Interpex IX1D Sounding Inversion software shows a resistivity low (~200 Ω·m) and a chargeability high (~7 ms) below 23 m depth. Measurements of total magnetic field using a Geometrics G-856 Proton Precession Magnetometer were modeled with Interpex IX2D-GM Magnetic Interpretation Software and constrained with magnetic susceptibilities of exposed rocks measured in the field using a SM-20 Magnetic Susceptibility Meter. Models based upon a portion of the data show anomalies of amplitude about 100 nT and wavelength about 50 m, suggesting isolated bodies of elevated magnetic susceptibility (~0.08 SI units) with upper surfaces 20-30 m below the surface. Since, based upon the topography, the depth to the water table is also 20-30 m, the geophysical measurements are consistent with the presence of reduced sulfide bodies below this depth. Further work will include interpretation of remaining data and possible drilling for improved calibration of geophysical models.
Influence of antibiotics on Gut Microbiota and Resultant Psychological Behavior
The normal flora of microbiota that resides in our gastrointestinal tract acts as a community and provides a number of functions such as assisting with the breakdown of waste, protecting our tissues and organs from invading species, and playing a role in the immune response. These microorganisms may also play a role in altering our brain chemistry and changing our psychology and behavior. This mechanism is considered to be due to their ability to produce neurochemicals that mimic those produced by our own bodies. Therefore, it is possible that antibiotics may have a detrimental effect on our gut flora, thereby inducing a host of undesired side effects. These described side effects may include changes in normal psychological behavior, such as the presentation of anxiety or depression. This hypothesis is increasingly relevant as antibiotic resistance is rising due to poor education regarding bacterial infections and as the appeasement of insistent patients continues. This research aims to illustrate the harm that over-medicating may have on our behavior as a result of afflicted gut microbiota. This research will use peer reviewed journal articles that include studies and experiments to determine the influence of gut microbiota on brain chemistry and therefore psychology and behavior. Specifically, the exact implications of consistent antibiotic use and the impact on gut microbiota will be examined and a correlation will be presented. Therefore, the purpose of this research is to illustrate this problem in regards to the healthcare industry and the over-medication of patient.
High-Frequency Ultrasound (20-80 MHz) for Analyzing Breast Cancer Surgical Margins: A 73-Patient Clinical Study
Results from a 2010 pilot study indicated that multiple parameters in high-frequency (HF) ultrasound spectra (20-80 MHz) correlate to a range of tissue pathologies in surgical margins from breast conservation surgery (BCS). One of these parameters, peak density, was particularly effective at discriminating between normal, atypical, and malignant patholUtah Conference on Undergraduate Research 2015 100 ogies. Subsequently, Utah Valley University and the Huntsman Cancer Institute initiated a follow-up study to further investigate this approach. Objectives: The purpose of this study was to determine the sensitivity and specificity of HF ultrasound for differentiating malignant tissue from normal tissue in BCS surgical margins. Methods: A 73-patient blind study was conducted with conventional pathology used as the gold standard for assessing the HF ultrasound method. Specimens were delivered by the surgeon’s team immediately following resection and ultrasonically tested outside the surgical suite. The margins were approximately 3x20x20 mm, and were oriented using a small staple inserted by the surgeon in one corner and a stitch on one side. The margin was tested at 2-5 locations on the specimens using our methodology and then sent to pathology for their analysis. 498 specimens were tested from 73 patients. That corresponded to 1112 positions collected from the margins. The data was then analyzed for malignancy using peak density, and then correlated with the traditional pathology. Results: Results from the current study indicate that peak density can differentiate between malignant and nonmalignant pathologies with an accuracy of 73.8%. The correlation between pathology and peak density has a high level of statistical significance compared to random chance, with p = 0.000078 (Fisher’s Exact test). The results also provide data for improving the technique. For example, approximately 3 times more false positives were observed than false negatives, indicating the peak density threshold used for identifying malignant pathology is most likely too low and should be adjusted to a higher value. Conclusions: Results from this study showed that HF ultrasound has the potential to provide rapid, intraoperative evaluation of surgical margins, thereby decreasing the number of additional surgeries for patients and thus increasing the quality and efficacy of surgical treatment for breast cancer.
Measuring the Effect of Breast Density on High Frequency Ultra sound using Tissue Phantoms
Breast density describes the proportion of connective tissue versus the fat tissue in the breast. Studies have shown that women with higher breast density are four to five times more likely to develop breast cancer than women with lower breast densities, (www.women.org/BreastCancer). Higher breast densities have proven to make current breast cancer imaging and detection more difficult. A pilot study done at the Huntsman Cancer institute showed that the ultrasonic parameter peak density, generated by high-frequency (HF) ultrasound (20-80 MHz), was sensitive to breast tissue pathology. The objective of this study was to determine the effect of breast density on ultrasound wave propagation from high frequency ultrasound using phantoms that mimic the histology of breast tissues. Phantoms were created from a mixture of distilled water, agarose powder, and 10X TBE stock solution. In order to simulate breast tissue histology and breast density, polyethylene microspheres were embedded into the phantoms in layers, totaling 4 layers per phantom. The polyethylene microsphere size (90-106 μm diameter) was kept constant within each phantom while the weight percent concentration of the microspheres varied (0.00g to 0.06g). Pitch-catch and pulse-echo measurements were acquired using 50-MHz transducers (Olympus NDT, V358-SU, 50 MHz, 0.635-cm diameter active element), a HF pulser-receiver (UTEX, UT340), and a 1-GHz digital oscilloscope (Agilent DSOX3104A). Glycerol (Genesis Scientific) was used as a coupling agent between the transducers and the phantoms. Spectra were derived from the data, giving peak density (the number of peaks and valleys in a specified spectral range), velocity, and attenuation values. The results showed that peak density did not start to show a trend until phantoms of 0.03g concentrations, where it increased from a value of 14.0 peaks (0.03g) to 18.7 peaks (0.06g). Velocity showed a statistically significant increase with greater polyethylene microsphere concentration, from 1508 m/s for 0.00g to 1536 m/s for 0.06g. No trends were observed for attenuation. These results indicate that higher levels of scattering centers in dense breast tissues will be detectable with high frequency ultrasound. This additionally shows that high frequency ultrasound may also be sensitive to greater amounts of connective tissue present in dense breast pathologies. High frequency ultrasound is sensitive to the weight percent of polyethylene microspheres. Future research is planned to further understand this relationship, including repeat studies and studies of phantoms containing chopped polyethylene fibers and triple the polyethylene microsphere concentrations to more closely simulate dense breast tissues.
Chernobyl and Fukushima
This study will compare and contrast the differences between nuclear accidents in Chernobyl and Fukushima. The environmental impacts of the “fall-out” across the two differing landscapes and the displacement of the populations due to radiation contamination, e.g., soil contamination, will be analyzed. Research on health issues, such as the increased numbers of thyroid cancer cases in Ukraine and Belarus in those who were children at the time of the disaster in 1986, will also be reviewed and compared to current health issues in Fukushima. Certain weather patterns distributed the radioactive materials over specific geographic areas that later came to be known as “hot-spots.” The study will evaluate the evacuations handled by the respective governments and the “exclusion zone” measures put in place by each. While the nuclear disasters at Chernobyl and Fukushima have been compared as similar in disaster level, research and data collection of the fallout zones, or hot spots, reveal that the two disasters are not of an equal level. Factors such as air temperature, political and social responses all contributed to the disparity in levels of the two disasters.
Spanish Advertising Use Relative to Median Household Income
According to the 2010 US Census, the Hispanic population in Ogden City, Utah has grown by over 36%. This influx of population of Hispanic descent has influenced local businesses to begin advertising in Spanish through multiple mediums. To determine what factors influence a business’s decision to advertise in Spanish, locations of all print advertising in Ogden were determined through personal investigation. Second, neighborhoods and areas were divided according to census divisions that appear on the official website and median household income as well as the population of Hispanic people was recorded to determine if socioeconomic bias was present in the selection of where to advertise in Spanish. A linear regression comparing the total number of Spanish advertisements found in each geographical area was compared first to income, and then to total Hispanic population. The results of the regression illustrate a potential bias based on financial circumstances rather than ethnicity. Further study is needed to determine if this use of Spanish-advertising in the low-income areas affects Spanish-speakers’ access to healthy foods, housing, or other critical aspects of quotidian life.
Analysis of Flavonoids, Catechins, and Proanthocy anidins in Cacao Chocolate
It is widely known that cacao beans are one of the most abundant sources of naturally-occurring flavonoids on earth. However, chocolate products contain only a small percentage of the original flavonoids present in cacao beans, indicating that up to 95% of these flavonoids are lost during the manufacturing of chocolate. However, no one has identified the specific events or steps in chocolate preparation that destroys these flavonoids. We have measured the concentrations of a variety of nutritionally beneficial flavonoids at each step of the chocolate manufacturing process to identify the related extent of flavonoid losses. Following multiple-step extractions and sample preparations, we utilized chemical techniques of TLC, UV/VIS Spectroscopy, HPLC, and organoleptic testing to measure the levels of catechins and proanthocyanidins at each step of the chocolate making process.
Validation of Metal Chelation by FTIR Spectroscopy
Nutritionally important minerals are more readily absorbed by living systems when they are combined with organic acids. These combined metal-organic acid complexes are called chelate metals or chelates. The synthetic processes utilized to prepare these mineral chelates adds significant cost to the final product. Occasionally, manufactures sell cheaper dry blends of unreacted minerals and organic acids to gain an unfair competitive advantage in the market place. There are few if any reliable methods for reliable measurement of the extent of chelation between metals and organic acids. We report our successful application of Fourier-transform Infrared Spectroscopy (FTIR) for the quantitative determination of chelation in solid samples of mineral chelates.
Effect of Roundup on Brine Shrimp (Artemia) Development
The herbicide Roundup and its active ingredient, Glyphosate, are widely used for weed control. These chemicals end up into streams and lakes, including the Great Salt Lake where it adversely affects wildlife. The goals of this project are a) to assess the mortality rate of Artemia larvae exposed to various concentrations of Roundup concentrate after a short exposure (48h) or a long-chronic exposure (7 days), b) to assess the effect of chronic on survival, maturation and fertility and c) to quantify the stress response of the shrimp on the heat-shock proteins 90 and 70. Materials and Methods: For the acute exposure, Artemia larval mortality was calculated in larvae exposed to Roundup concentrations ranging from 10-3 to 10-10 ml/l of Roundup concentrate for 48 h. For the chronic exposure, larvae were raised in the above Roundup concentrations. Mortality, maturation and fertility rates were calculated. The response to stress was assessed by quantifying the up-regulation of stress proteins hsp90 and 70 using western blots. Results: All larvae were killed after exposure at 10-4 g/l or greater of Roundup concentrate. Most larvae survived at Roundup concentrations of 10-6 ml/l or less. While chronic exposure to lower Roundup concentrations did not seem to affect survival or maturation rate, it did affect larval development. Larvae developing in 10-7 ml/l or more Roundup had about a 20% risk of not hatching or dying shortly after hatching. Hsp70 western blots showed an upregulation of this heat-shock protein at 10-5 ml/l or higher Roundup concentrations.
A Plan for Complete Recycling of Stormwater on the Utah Valley University Main Campus, Orem, Utah
Evaluation of Utah Valley University’s stormwater plan reveals a simple system meant to collect stormwater into the city storm drains as quickly as possible. It is, however, vastly underdeveloped and many unspectacular summer and springtime storms have resulted in property damage, including those of nearby residents. The stormwater runoff has also collected concentrated amounts of hydrocarbons, nitrogen and heavy metals which are being fed directly into Utah Lake, acting as a significant source of pollution for the lake environment. The intentions of this project are to design a stormwater management plan that can withstand a 100 year, 24 hour event and prevent pollutants from entering the Utah Lake system. Mapping and modeling of the University’s storm drains will be accomplished using GIS as well as modeling for efficient retention sites on campus. Captured stormwater will then be used for a variety of functions here on campus and runoff into the adjacent lake will be reduced to insignificant values. Decisions regarding the ultimate implementation of this project will work in concordance with the University’s master plan of future development in order to realistically secure a reliable, low-maintenance system.
Mobility and Distribution of Trace Element Pollution in Sediments of the Utah Lake Outlet
The Jordan River is the only outlet of Utah Lake. Historically, this area has been impacted by urbanization, long term mining operations, industrial and agricultural activities resulting in potentially high levels of trace metal pollutants at the headwaters of the Jordan River. Since trace metals are known to be toxic at elevated levels, it is important to evaluate their concentration, distribution and mobility in this sensitive area in order to determine risk to wildlife, humans and downstream users of the Jordan River. Three core samples from 0 cm to 95 cm in depth were collected at the east (industrialized area), west (newly developed area) and north (an island barrier) sides of the outlet of Utah Lake. Each sample was digested in triplicates in the Microwave Accelerated Reaction System (MARS) using US Environmental Protection Agency Method 3052 and analyzed in the Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) for As, Cd, Cr, Mn, Pb, Cu, Zn, Co, and Ni. A multivariate analysis of variance (MANOVA) was used to analyze the data, with a boneferroni adjustment made for multiple comparisons. The results indicated that the East and North sediments of the Jordan River were significantly (P < 0.5) more heavily impacted by trace metal pollutants than the West side, with the North area accumulating higher levels of the majority of the trace metals or metalloid evaluated. Enrichment of Co, Cr, Pb and Zn was observed on the East sediment at concentrations reaching 10,821, 4.07, 13.7, 12.7, 119.4 mg kg-1, respectively. The most substantial elevation in the concentration of trace metals occurred with Cu in the North section of the lake, increasing from 349 mg kg-1 at 0-15 cm to 1383 mg kg-1 cm depth, showing high mobility, followed by Zn which increased from 46.7 mg kg-1 at 0-15 cm to 592 mg kg-1 30-45 cm depths. The enrichment of these trace metals in the East and North sediments at the outlet of Utah Lake pose a health risk to animals and humans who use these areas for recreational or agricultural purposes.
High-Frequency Ultrasonic Measurement of Angiogenesis in Mice with Breast Tumors and Ligated Femoral Arteries
Breast cancer is the most common cancer among women in the United States. Tumor angiogenesis and its inhibition is an important aspect of oncology and the treatment of cancer. High-frequency ultrasound (10-100 MHz) is particularly sensitive to small vascular structures that are close in size to the ultrasound wavelength (15-150 _m). The ability to rapidly determine the degree of vascularization in small animals in vivo would provide a useful characterization tool for breast cancer studies. The objective of this study was to determine if direct ultrasonic measurements in the 10-100 MHz range could be used as a vascularization assay for breast tumors and other tissues. To accomplish this, six mice from the Huntsman Cancer Institute (Salt Lake City, Utah) with grafted breast cancer tumors (three control and three treated with an angiogenesis inhibitor called Avastin) were tested in vivo using through-transmission ultrasonic measurements. A second study was also performed at the Ludwig Boltzmann Institute for Experimental and Clinical Traumatology (Vienna, Austria), where the femoral artery in one hind leg of each of sixteen mice was ligated and tested over the time period of eight days. Eight of the ligated limbs were treated with vascular endothelial growth factor (VEGF) while the remaining eight ligated limbs were allowed to grow ischemic. The unligated limbs were controls. Results from the Huntsman Cancer Institute study indicated that breast tumors in Avastin-treated mice showed higher ultrasound velocities than control tumors. This can be ascribed to the vasculature in the nontreated tumors creating greater wave scattering in the tissue, thus decreasing the velocity. Results from the Boltzmann Institute study indicated that in mice with ligated femoral arteries, ultrasonic signals from ischemic limbs displayed a decrease in wave velocity over the test period as compared to the VEGF-treated limbs. However, both the ischemic and VEGF-treated limbs showed decreases in ultrasonic attenuation during the entire test period. Results from Avastin-treated mouse tumors and mouse limbs with ligated femoral arteries revealed that high-frequency ultrasound holds potential for measuring angiogenesis in vivo.