2020 Abstracts

Jensen, Mia (Utah State University)
Faculty Advisor: Kinkead, Joyce (College of Humanities and Social Sciences, English Department)

Undergraduate English majors are constantly bombarded with writing heavy assignments. Although these students are some of the most comprehensive writers in the university, several are still apprehensive about their writing assignments. Meditation (active mindful breathing), is increasingly growing traction in the mental health community as a means to lower stress. Could students utilize the calming effects of meditation to improve their apprehension towards writing? The study conducted aims to identify what relationship if any, meditation has on the journals of English majors at Utah State University. Eight English majors gathered together and wrote in a journal for ten minutes. Afterward, they listened to a ten minute guided meditation practice followed by another ten minutes of journaling. Finally, the students answered a Qualtrics survey about their experience. The research sought to answer questions like, "did their vocabulary change?", "did they feel more or less apprehensive writing the second journal?", and, "could they focus more or less on the second journal?". Could the results influence students and teachers alike to include meditation in their pre-writing rituals?

Johnson, Marian (Utah Valley University)
Faculty Advisor: Abunuwara, Kim (Utah Valley University, Integrated Studies); Dunn, Paul (Utah Valley University, Biology); Oliveira, Andre (Utah Valley University, Economics)

Current American perceptions of ecological systems are toxic to a relationship with the natural world. Human-led extinction rates are unsustainable and obliterating untold amounts of diversity which could potentially help humans. A lack of appreciation for the intrinsic value held by non-human species contributes largely to cultivating an ignorant relationship between humans and their surroundings. The value of non-human species is currently difficult to calculate. Many species are not noticed until they are threatened or extinct due to their invisibility in the context of America's heavy emphasis on monetary value. Expecting an emphasis to be placed on ecological diversity without proper education on the significant long-term ramifications will hold no weight for individuals, corporations, and policy-makers. A cultural shift must be initiated before concrete policies can be implemented. Several aspects of this cultural shift are exposing value in species who are annoying, ugly, or seemingly give nothing to humans. Invisible species are some of the quickest to be swept away in ecological ignorance, contributing heavily to loss of diversity. The focus of this thesis is to suggest potential cultural tools in making this shift. Indigenous cultures which possess a sustainable and mutualistic relationship with the natural world ought to be explored as a model for which the United States can take steps to adopt. Fragments of American culture such as social media, monetary gains, tax incentives, and new additions to the public school curriculum are potential tools in strengthening and informing a realistic ecological opinion.

Truman, Jorden (Dixie State University)
Faculty Advisor: Pilkington, Olga (Dixie State University, English)

This paper uses literary analysis and theoretical framework of Regionalism to examine Barbara Kingsolver's novel Flight Behavior. Flight Behavior features Dellarobia, an inhabitant of a poverty-stricken Appalachian town, who discovered that a hill in her backyard became home to millions of wintering Monarch Butterflies. This abnormal flight behavior of the Monarch Butterflies for many in Dellarobia's community is seen as a gift from God, but for the outsiders, it is an ominous sign of climate change. The results of my analysis show that Kingsolver, although praised for her other novels set in the Appalachias, fails to address what Regionalist novels are criticized for: preventing the reader from taking sides with the educated master narrator against the abnormal or aberrant natives. Kingsolver's pursuit of the moral imperative comes at the cost of disparaging and humiliating the communities that makeup Appalachia. The ramifications of Flight Behavior are troubling because this "if you are not with us, you are against us" style of narration is prominent in climate change activist's discourse: creating enemies instead of allies.

Witham, Arianna (Dixie State University)
Faculty Advisor: Pilkington, Olga (Dixie State University, Applied Sociology)

Two of Shakespeare's plays Much Ado About Nothing and Hamlet have plots that are largely dependent on the death of a female character. Looking at the deaths of Hero and Ophelia though the lens of feminist literary analysis shows that these deaths are transformative points. On the surface, these deaths are very different: Ophelia stays dead while Hero's death is only a ruse. However, the deaths of Hero and Ophelia transition both of them from persons to objects. In the case of Ophelia, after death all that is left is a corpse, and Hamlet and Laertes' altercation in Ophelia's grave on top of her body contradicts their sorrowful proclamations just before. After Hero re-enters society, admittedly as someone else, the only thing about her new identity that seems to matter is her physical similarity to the Hero most believe to still be dead. Considering genre prompts another comparison between these deaths. In Much Ado About Nothing, Hero's death prompts Beatrice and Benedict's confessions of love, and her return to society allows the weddings and celebration that finish the play and mark it as a comedy to happen. Tragedies are marked by the death and destruction of the characters, and Hamlet is no exception. Ophelia's death is a catalyst for events that lead to the deaths of the other characters. If she had been revived, then there would be no funeral, and the emotional stakes at the end of the play would not be as high. The deaths of both Ophelia and Hero serve to drive the plots of these plays, but at the cost of the personhood of each.

Barker, Avery (Utah State University)
Faculty Advisor: Kinkead, Joyce (College of Humanities and Social Sciences, English Department)

Clothing is a large part of a person's identity, it allows a consumer to express who they identify as outwardly. The demand for clothing that people identify has increased immensely over recent years. The industry is capable of producing mass amounts of clothing of all styles, yet we do not see a prominent amount of apparel that references books readily available to the consumer. That is why we will be looking at apparel that displays references to literature such as published works. By researching into this apparel, we will be able to determine what the literary apparel industry is, how it is growing, why we are seeing it now, how popular this apparel is, and how it relates to us. To answer these questions, we will be conducting surveys with English Majors here at Utah State University, interviewing professionals in industries that relate to literature apparel, and reviewing literature pertaining to apparel.

Bresee, Andrea; Abel, Emily; Adams, Roland; Ashby, Shelby; Barker, Avery; Borden, Eden; Christensen, Nikki; Eralie, Megan; Evensen, Cayenne; Haney, Cameron; Jensen, Mia; Jensen, Raychel; Julander, Alexis; Pulsipher, Chase; Roberts, Katie; Roundy, Talia; Schroeder, Janell; Wheeler, Shylee; Wood, McKenzie (Utah State University)
Faculty Advisor: Kinkead, Joyce (College of Humanities and Social Sciences, English Department)

Although university students who major in English studies write consistently, often the writing behaviors are taken for granted, particularly the long-term writing lives of these students. How did they develop as writers? What strategies have they developed to be successful writers? What are their preferred tools and technology, the material culture of writing? How has their various cultures influenced their writing? Autoethnography is a type of qualitative inquiry that can help construct and analyze identity through both process and product. The parts of the term indicate what it means: graphy, referring to writing, also means to graph, describe, and analyze systematically one's personal experience � the auto as in autobiography or autograph. Ethno refers to how a person is placed within a cultural experience. How is the personal experience a reflection of culture or subculture? By using an autoethnographic approach, including intensive analysis of a week's writing, surveys, and interviews, that describes and interrogates their processes and products, the researchers, who are also the subjects, develop a profile of the writing lives of upper-division English majors at a land-grant, research university.

Sorensen, Brianne (Utah State University)
Faculty Advisor: Edenfield, Avery (College of Humanities and Social Sciences, English Department)

I investigate how #burkaban is used by Twitter users to point out the compounded marginalization of Muslim women in modern Western law, particularly Denmark, through discriminatory practices in government.
Historically, Denmark has a fewer number of Muslim communities compared to other modern Western societies. I chose to research Denmark because Muslim communities are already severely marginalized socially, economically, and physically. Denmark also uses discriminatory speech in their lawmaking to intentionally discriminate against Muslim communities.
I unpack and use the framework of Crenshaw's ideas on intersectionality from "Demarginalizing the Intersection of Race and Sex," but instead of demarginalization of the intersection of sex and race, I examine how hashtag use points out the marginalization of the intersection of sex and religion. I also use Koerber's "Toward a Feminist Rhetoric of Technology" to bridge the gap between how technology research intersects with discriminatory political practices. I utilize Frost's Apparent Feminism methodology framework to analyze how hashtags are used to address political trends that are intentionally discriminatory, particularly towards Muslim women. I further utilize this methodology to look specifically at how this hashtag is used to pose an intervention of discriminatory politics.
My conclusions determine who is using the #burkaban on Twitter, whether it is Muslim women or allies of Muslim women. I argue different hashtag users deploy #burkaban towards different goals for political intervention in Denmark. I also conclude and argue that Twitter has an ethical responsibility to take action towards political intervention in Denmark.

Kaneshiro, Alma; Jordan, Adam; Crompton, Rhees; Brailsford, Samantha; Spencer, Jonathan (Weber State University)
Faculty Advisor: Clark, Daniel (Science, Microbiology Department and Neuroscience Center); Chaston, John (Life Sciences, Plant & Wildlife Sciences)

Antibiotic resistance is of great concern in the medical community, with bacterial resistance increasing proportional to their use. Staphylococcus aureus, such as methicillin resistant S. aureus (MRSA), can cause fatal infections. Problems due to this resistance are compounded when the infecting bacteria form a biofilm, thick sticky layers of bacterial secretions, which are difficult for antibiotics to penetrate. Biofilm formation is common in hospital settings on stents, catheters, and IV lines. Biofilms make antibiotic treatment risky due to incomplete killing—the most resistant survive exposure. There is evidence that bacteriophage can break up biofilms, possibly making them more susceptible to antibiotics. We induced a S. aureus biofilm formation using chemicals that mimic a skin wound. Using bacteriophage K, we inoculated the biofilm and observed clearance. Samples of cell pellets and liquid supernatant were collected, and DNA was extracted. Real-time PCR was used to quantify the levels of bacteriophage K replication, representing clearance of the bacteria. This research can be used to find efficient ways to treat an infection caused by a S. aureus biofilm. Bacteriophage used in combination with antibiotics may be able to better clear a biofilm infection and reduce antibiotic resistance risk due to more complete infection clearance.

Townsend, Jessica; Freitas, Claudia; Weber, Scott; Cardon, Dallin (Brigham Young University)
Faculty Advisor: Weber, Scott (Brigham Young University / Life Sciences, Microbiology and Molecular Biology)

The World Health Organization reported in 2016 that oral diseases affected half of the world's population. Oral diseases are due to poor oral hygiene and tobacco use which can develop into periodontal disease. Periodontal disease is caused by an immune response to microbial challenge, which initiates an invasion of lymphocytes and other single-nucleated cells to the site of inflammation in the mouth that can cause tooth loss and is a risk factor for heart and lung disease. Patients with severe periodontitis have increased auto-reactive B lymphocytes that express the CD5 co-receptor and these cells are influenced by T cells. We propose to investigate the relationship between oral inflammation, CD5, and the T helper immune response. This will be done by comparing oral inflammation in mice with and without CD5. CD5 is a T cell co-receptor that regulates T cell development and function and we hypothesize CD5 plays an important role in periodontal disease. We will test this hypothesis by co-culturing T cells expressing or lacking CD5 with oral mucosal or gingival epithelial cells that have been exposed to LPS (lipopolysaccharide, a major component of gram-negative bacteria's wall) and will exam differences in cell number, T cell subtype, and cell function.

Spruance, Lori; Augustine, Madi (Brigham Young University)
Faculty Advisor: Spruance, Lori (Life Sciences, Public Health)

Youth sport programs are an opportunity to increase physical activity, but the food environment may be detrimental to improving and maintaining health. From a previous study, parents indicated that they would like guidance and direction in a top-down approach from coaches and administrators; yet, understanding the administrator experience relative to the youth sports food environment remains unclear. The purpose of this study is to understand that experience. Semi-structured qualitative interviews will take place with administrators across the state of Utah. Interviews will be recorded and transcribed. Thematic analysis will be conducted to identify salient themes. A peer-reviewed publication and multiple presentations will result from the study conducted.

Haynie, Christopher; Freitas, Claudia M. Tellez; Whitley, Kiara V.; Weber, K. Scott (Brigham Young University)
Faculty Advisor: Weber, K. Scott (Life Sciences, Microbiology and Molecular Biology)

T cells play a critical role in the adaptive immune response and undergo significant metabolic changes upon activation. T cell co-receptors influence T cell activation and function, yet their influence on T cell metabolism remains unclear. CD5, an inhibitory co-receptor expressed on the surface of T cells, is known to regulate thymocyte selection and T cell receptor (TCR) signaling. We previously observed that CD5 plays a critical role in calcium signaling in naïve helper T cells. As calcium signaling influences metabolic changes in cells, our current work focuses on understanding the role of CD5 in T cell metabolism. To understand how CD5 regulates metabolism in T cells, we used CD5 deficient T cells and compared them to wildtype CD5 sufficient T cells. We have characterized their metabolic activity using glycolytic and mitochondrial respiration assays. Interestingly, CD5 deficient naïve T cells have increased glycolysis, mitochondrial respiration, and spare respiratory capacity in comparison to wildtype T cells. We hypothesize that this is due to CD5 altering mitochondrial membrane potential and mass, gene regulation, and the influence of different cellular fuels. Understanding how CD5 regulates T cell metabolism will provide critical insights for improved immunotherapeutic strategies.

Edwards, Jeffrey; Friend, Lindsey; Weed, Jared; Sandova, Philipl; Nufer, Teresa; Ostlund, Isaac Ostlund (Brigham Young University)
Faculty Advisor: Edwards, Jeffrey (Life Sciences, Physiology and Developmental Biology)

Substance abuse is a widespread problem in the United States. Although there are some existing treatments for addiction, the neural mechanisms of addiction are not deeply understood. This study quantifies the expression of GAD65 and GAD67 in GABAergic cells in the VTA of adolescent mice to shed light on the subtypes of cells involved in learning and addiction pathways.

The ventral tegmental area (VTA) of the brain, a critical part of the dopamine reward system, has many dopamine cells that are inhibited by nearby GABAergic neurons. Formation of memories and addiction involve long-term potentiation (LTP) and long-term depression (LTD) of these inhibitory GABA cells. We studied potential pathways of learning and addiction by measuring levels of expression of GAD 65/67 proteins and quantifying the cells that express one or both of these proteins.

Our results will provide insight about which GABAergic neurons are involved in the addiction pathway, furthering our understanding of the cellular mechanism of addiction. This will pave the way for more educated, effective treatment of drug addicts in clinical settings.

Pehkonen, Eliza (Salt Lake Community College)
Faculty Advisor: Seaboch, Melissa (Salt Lake Community College, Anthropology)

Precipitation-associated behaviors have been observed in several species of primate including bonobos (e.g., building leafy shelters), chimpanzees (e.g., drinking, rain dancing displays), and mantled howler monkeys (e.g., licking rain from the air, altering typical behavior based on weather and season). The purpose of this study is to determine if mantled howler monkeys (Alouatta palliata) exhibit precipitation-associated vocalizations. A. palliata is well known for its vocalizations, which are the loudest sound made by any terrestrial mammal and are used for a wide variety of communicative purposes, such as attracting mates, defending territory, and deterring predation. Given the purpose with which A. palliata vocalizes and the existence of precipitation-associated behaviors within primate species, including A. palliata, it was hypothesized that A. palliata would vocalize in association with climatic events (precipitation and thunder). To test this hypothesis, 41.75 hours of data were collected on A. palliata over a two-week time period during the rainy season at La Selva Biological Station in Costa Rica. All-occurrence sampling was used to record the timing and duration of all A. palliata vocalizations, precipitation, and thunder events. Events were considered accompanied if they occurred within five minutes of one another. Of the 59 observed vocalization events 53% were associated with climatic events. Of the 20 observed precipitation events 90% were accompanied by vocalizations and of the 37 observed thunder events 57% were accompanied by vocalization. Associated vocalizations occurred before, during and after climatic events, however, during or after were most common. The data indicate an association between A. palliata vocalization and precipitation, confirming the hypothesis. Further research is warranted to investigate a possible purpose of precipitation-associated vocalizations, an understanding of which could provide further insight into A. palliata's behavioral interaction with climatic events.

Smith, June; Mishra, Niharika (Weber State University)
Faculty Advisor: Oberg, Craig (Weber State University, Microbiology); Culumber, Michele (Weber State University, Microbiology)

Non-dairy food options have become a growing cultural necessity, however, providing fermented or probiotic supplemented non-dairy alternatives is difficult. Little is known about the activity and survival of probiotic cultures in dairy alternatives. We evaluated the activities of several probiotics at various concentrations and in different combinations in oat, almond, and coconut beverages. Probiotic culture strains of Streptococcus thermophilus (YFLO1), Lactobacillus rhamnose (LGG), L. casei (Casei 431), and Bifidobacterium animalis subsp. lactis (BB12), and commercial probiotic mixtures, YFLO2, and Fresh Q, were inoculated in MRS broth, transferred to MRS agar plates, and incubated anaerobically for 24 hours at 37_. BB12 was grown anaerobically in MRS + cystine broth and agar. Isolated colonies were assayed on API 50 CH panels, and a carbohydrate use panel was developed for each organism. Oat, almond, and coconut beverages were inoculated in duplicate with the isolated strains and incubated in a water bath at 40_. The pH was recorded at regular intervals for up to 41 hours. The oat beverage had the most rapid and significant pH change, when incubated with either YFLO1, casei431, and LGG, dropping between 1.5 to 3 pH units over 3 hours depending on the culture. The almond and coconut beverages did not show rapid pH change with the organisms tested. Due to the quick decrease in pH change, further tests on the oat beverage. It was inoculated with Lactobacillus casei 431, LGG, and YFLO1. Organisms were tested at 0.5%, 1.0%, and 2.0% concentrations in oat beverage in triplicate. These inoculations were again incubated at 40°C and pH monitored after 5 hours, then plated on MRS agar plates after 24 hours. Final ranged between 1.0 x 109 - 1.8 x 109 for the 1% inoculum. It appears that these organisms survive, and may even grow in the oat beverage. This research demonstrates that probiotic cultures can grow in non-dairy beverages and can ferment the available carbohydrates and decrease pH. These results provide insights that can be used for beverages, yogurt, ice cream, and other fermented food production.

Armitstead, Annie; Grether, Lara; Creech, Kealani (Brigham Young University)
Faculty Advisor: Watt, Richard (Brigham Young University, Biochemistry)

Lateral Flow Immunoassays (LFI) are simple tests that detect specific levels of antigens or antibodies in patient samples. Requiring only a few minutes, small sample sizes and no read-out equipment, LFI�s are an invaluable and time efficient testing technique. Made up of multiple layers they facilitate the capillary flow of a sample through protein detection zones and can be developed to detect virtually any disease or condition.

Despite the attractive attributes of these tests, the assembly of an LFI strip requires expensive machines, trained personnel, and materials not easily accessible to low-resourced labs or clinics. Developing an innovative point-of-care platform designed to be streamlined, low-cost, and intelligible to the unskilled would open the door of medicine to even the most underprivileged clinics in the world.

We are currently developing a paper LFI that uses a single sheet of copy paper with the ability to filter whole blood as well as replacing high-priced machines with stencils and pens which can still deliver detection proteins to the designated test zones. This avenue of testing is supported by previous experiments we have done with protein pens and tagged antibodies. Using anti-mouse and anti-hCG antibodies as our control and test lines respectively, we spike our sample with hCG mouse antibodies tagged with nanoparticles, and we are able to see binding of both proteins with their respective antibodies. We have seen results in our new testing technique that is easily comparable with currently commercialized LFI's: visual results of binding within 1 min, successful transformation of printer paper into a functional binding platform, and consistent protein binding at a 1/10^5 concentration. Once this concept can be translated to different inks in order to diagnose a plethora of varying conditions, we'll be able to detect diseases and other important biomarkers no matter the limiting low-resource circumstances.

Larson, Rebecca; Jimenez, Misty (Utah Valley University)
Faculty Advisor: Jensen, Francine (Utah Valley University, Nursing)

Stress is a known barrier to patient recovery. Patients experience increased emotions, such as stress, while hospitalized due to high stakes from risks to life, health and well-being. Patients' emotions can affect their perceptions, future intentions, and behaviors. In pediatrics, the way parents react to their child's illness may affect the children's compliance, emotional response to medical treatment, and even some development processes, demonstrating the premise that there are many possible stressors that can have significant impacts on patients. Hospitals have taken several measures to evaluate patient stress, such as encouraging hospital staff to discuss patient satisfaction surveys with their patient. However, not all patients recognize their own stressors, and some patients may not initially feel comfortable sharing them. For example, a study showed specific stressors that may experienced by patients of different demographics. These stressors may not always be apparent to nurses. Patients' stress can be reduced if the hospital environment fosters perceptions of control, social support and positive distraction. A change in patient environment can promote healing, as evidenced by a hospital, Navicent Health, that demonstrated in their neonatal intensive care unit that reducing stress and anxiety for both newborns and their parents facilitated healing growth and bonding. Nurses can improve the care they provide to patients by learning how to recognize and reduce stressors during the hospital stay.

Davis, Morgan; Edvalson, Logan; Busath, David (Brigham Young University)
Faculty Advisor: Busath, David (Life Science, Physiology and Developmental Biology)

Influenza infection, and subsequent pneumonias, are the cause of over fifty thousand deaths in the United States per year, and, according to the CDC, influenza is the 8th leading cause of death in this country. Research into the pathogenesis of influenza elucidates critical interactions that take place during different phases of infection which can be targeted by novel drug therapies. Our lab has focused on discovering the role of of PDGFR and VEGFR and other Receptor Tyrosine Kinases (RTKs) in aiding viral infection. RTK activation is reported to be important for successful viral infection, and our project has focused on three different RTKs: VEGFR, PDGFR, and endothelial growth factor receptor (EGFR). In these experiments, Madin Darby Canine Kidney (MDCK) cells were bathed in growth medium containing a specific RTK inhibitor, and then infected with the influenza virus. The vitality of the cells was measured using crystal violet staining and spectrophotometer results. The data showed that using a drug called imatinib—a potent PDGFR inhibitor—resulted in the highest cellular vitality while VEGFR inhibitors developed here at BYU also showed anti-influenza activity. This suggests that the influenza virus is at least partially dependent on PDGFR and VEGFR activation to enhance its life cycle. Future experimentation will study which of the many branches of these receptor's phosphorylation cascades are being utilized by the virus.

Edwards, Taylor; Ram, Akila; McCarty, Ashley; Bobeck, Erin N (Utah State University)
Faculty Advisor: Bobeck, Erin (College of Science, Biology Department)

First-line chemotherapies against solid tumors are highly efficacious in reducing the tumor burden, but have many adverse side-effects including nerve damage, leading to chronic pain. Non-addictive, efficacious pain relievers are an area of active interest, and we propose a novel target to address this pressing issue. GPR171 is a G-Protein Coupled Receptor that was recently deorphanized and was identified to be expressed in the brain in regions that regulate reward, anxiety, and pain. Within the pain circuit, it was shown previously that systemic administration of the GPR171 agonist enhances morphine antinociception in acute pain tests. Preliminary data from our lab has shown that GPR171 activation can also alleviate persistent inflammatory pain. However, the role of this receptor has not been investigated in other chronic pain models. Given these findings in acute and inflammatory pain, we hypothesize that GPR171 can reduce neuropathic pain. To test this hypothesis, we investigate the role of GPR171 in chronic neuropathic pain. We tested the efficacy of a GPR171 agonist in a chemotherapy-induced neuropathy mouse model. Neuropathic pain was induced by injecting paclitaxel (16 mg/kg) followed by assessment of the pain-relieving effects of activating GPR171 receptors. Mechanical pain thresholds were assessed using Von Frey filaments. We observed an increase in mechanical thresholds following GPR171 agonist treatment. Further, using immunofluorescence we observed that there is a decrease in GPR171 receptors in the periaqueductal gray (PAG) of these mice that have neuropathic pain, indicating that the agonist can bind to the available receptors to produce pain relief. Overall, this study proposes that GPR171 may be a novel target for the treatment of neuropathic pain.