2020 Abstracts

Rasmussen, Melissa; Lauritsen, Jake; Clarke-Midura, Jody; Lee, Victor; Recker, Mimi (Utah State University)
Faculty Advisor: Lee, Victor (Emma Eccles Jones College of Education & Human Services, Instructional Technology and Learning Sciences Department); Recker, Mimi (Emma Eccles Jones College of Education & Human Services, Instructional Technology and Learning Sciences Department)

With the rising perception of computer science as a universally useful skill in the twenty-first century, researchers and schools test ways to effectively interest and introduce young children to computer science. Our project develops a curriculum pairing an "unplugged" board game component with a "plugged" portion in MIT's Scratch, pairing short class lectures with hands-on student learning. In order to effectively work with our partner school district and implement a complete unit without taking too many class minutes, we integrate much of the hands-on portion of the curriculum into existing library time. We run several iterations of the curriculum for fifth graders, refining the 6-8 week unit as we go. Of particular concern in our design is the typical middle school teacher's unfamiliarity with coding and computer science concepts. We find that it may be possible to capitalize on this unfamiliarity in order to improve student outcomes. We look at student creativity and computational thinking during the unit, seeking an increase in ability and self-efficacy by closing the unit with student creation of their own game boards. Furthermore, we examine female engagement and interest in the programming portion, with the goal of pinpointing factors that could increase female participation in computer science or partially explain the current deficit. The results of the implementation of our curriculum, with particular focus on the successes and failures that can be generalized to other computer science curricula, will be discussed.

Perisse, Iuri Viotti; Fan, Zhiqian; Wang, Zhongde; Harris, Ann; White, Kenneth L.; Polejaeve, Irina A. (Utah State University)
Faculty Advisor: PoleJaeva, Irina (College of Agriculture and Applied Sciences; Animal, Dairy, and Veterinary Sciences Department)

Cystic Fibrosis (CF) is a recessive human genetic disease that is caused by mutations in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene. This gene is responsible to transport Cl- and HCO3- anions in epithelial cells. Previously, we generated CFTR-/- lambs using CRISPR/Cas9 and SCNT techniques. The CFTR-/- lambs display many features similar to human CF disease, including meconium ileus (MI), pancreatic fibrosis, portal fibrosis and biliary hyperplasia, small gallbladder, and absence of vas deferens. In CF patients, MI affects only 15-20% of human babies, whereas it was observed in 100% of newborn CFTR-/- lambs and was the primary cause of death. We here hypothesized that the transgenic expression of the ovine CFTR cDNA under regulation of an intestinal-specific expression promoter would promote the correction of MI in CFTR-/- sheep. In this study, we are constructing three potential vectors with different promoters to be evaluated prior to the generation of transgenic animals. Rat intestinal Fatty Acid Binding Protein (iFABP), rat liver Fatty Acid Binding Protein (LFABP), and Villin1 promoters have already been characterized and successfully used for intestinal-specific expression. After digestion and ligation cloning, the three constructs will be sequenced to confirm the presence of all segments (promoter, cDNA, and vector) in the correct orientation. Subsequently, we plan to evaluate the transient gene expression of the constructs in CaCo-2 cells to ensure they are fully functional. Therefore, we will construct the pcDNA3.1>promoter>CFTR expression vector in order to generate intestine-CFTR transgenic CFTR-/- sheep.

Colbert, Jeffrey (Weber State University)
Faculty Advisor: Schramm, Katherina (Weber State University, Botany); Hillbig, Bridgette (Weber State University, Botany); Walker, Edward (Weber State University, Chemistry)

Hemp is a multimillion-dollar industry; however, it is only grown in a few states in the US. In prehistoric time, there was a brackish lake that covered the valley of Utah�s Wasatch Front. Because of this lake and different drainage events, the soil chemistry and composition changed depending on the depth and salts concentration of the lake.
This study tested soils from three different locations along the Wasatch Front for their potential to support healthy growth of hemp plants. Mg, Ca, N, P, K concentrations were tested along with pH in soils from different geological strata. In an outdoor environment hemp was grown in five-gallon containers to determine if those soils will support hemp growth yielding high fiber content and quality. Potting soil was used as a control. The same soil characterization tests were conducted after the plants were harvested to show what amendments are needed for a successful crop in following seasons. Mature plants� inflorescences were tested for tetrahydrocannabinol (THC), and cannabidiol (CBD) levels by Utah Department of Agriculture and Food (UDAF). All plants contained less than 0.3% THC. Fiber quantity was measured using confocal microscopy techniques and compared against the different soil collection locations. Water retting was conducted to extract fiber from stalks. Thin Layer Chromatography (TLC) was conducted confirming UDAF�s findings of THC and CBD.

Mary Lay Schuster, Mary Daly (Utah State University)
Faculty Advisor: Edenfield, Avery (College of Humanities and Social Sciences, English Department)

There have been recent developments in reproductive technologies that are staking claims to better the lives of women. The invention and development of reproductive technologies alone is not enough to classify them as technologies aiding women, however. Ultrasound, IVF, and breast reduction technologies specifically can be as helpful to women as they were intended. Ultimately, the way they are put into practice, viewed by society and used by professionals can inadvertently make women feel more marginalized. A woman's agency is diminished by ultrasound technology as she is debating having an abortion; a woman's involvement in her own IVF-aided pregnancy can be positive or negative depending on the outcome; a woman's breasts can contribute to her femininity so long as they fit societal standards. As a technical communicator, I feel a responsibility to bridge this gap between technology and humanity because the two need to function together without one negatively impacting the other. Through my research I will prove this by drawing on the work of Mary Daly and her theory of a rhetorical foreground and background (spaces where women are oppressed and spaces where the oppression is removed, respectively) while analyzing the findings presented by Mary Lay Schuster in her article "The Rhetoric of Reproductive Technologies." Throughout this analysis, I would like to not only point out the problems women are currently encountering with different reproductive technologies, but also to suggest ways to amend the situation moving forward. Mary Lay Schuster points specifically in her article towards the practice of midwifery, and so one of my solutions would include utilizing modern medicine in combination with midwifery.

Webb, Kortney (Dixie State University)
Faculty Advisor: Jeffreys, Mark (Education, Interdisciplinary Arts and Sciences)

This paper applies Imagined Interaction Theory to the dramatic monologue. Imagined Interaction (II) refers to a cognitive state in which people daydream about social interactions.
These II's can be used as practice for an actual conversation with a person. There are six functions of II's self-understanding, compensation, catharsis, rehearsal, conflict-linkage, and relational maintenance. Each of these functions shows why an individual would participate in imagined interactions. There are also mental ways II's represent themselves including self-talk, daydreaming, focus on organization of thoughts, and II can also be used as a therapy tool. How does this theory relate to the dramatic monologue? II's aspects are visible in how a dramatic monologue is displayed. Although II happens inside the head, the actors have to bring their internal monologue into speech through dramatic monologue. Here, II theory applies when performing a dramatic monologue directly in II form. Despite the controversy around the definition of the dramatic monologue, II aspects can be seen through the criteria and how the dramatic monologue is displayed. For practical application this research will apply findings to the dramatic monologue by performing a monologue(s) in II form. This research is significant because this topic is not researched sufficiently and it shows how the performance of the dramatic monologue in theater can mimic real world communication in various situations.

Kaundal, Amita; Hansen, Nathaniel; Ganesh, Jyothsna (Utah State University)
Faculty Advisor: Kaundal, Amita (College of Agriculture and Applied Sciences; Plants, Soils, and Climate Department)

Plants are sessile and bound to their origin so they cannot move to defend themselves against adverse environmental conditions. Soil salinity is one of such environmental stress, which limits the growth and development of plants. Salt stress directly affects crop production. It has been predicted that by 2050, about 50% of arable land will be affected by salinity. About 23% of the worldwide farmland is affected by soil salinity and the crop losses due to salinity are estimated to be tens of billion dollars per year. On top of that, an increase in the world population, the demand for food production also increases. Thus, feeding the growing population under adverse conditions is a challenge. This challenge demands to create more resilient crops to adverse conditions and to feed the growing population. In this study we are investigating General Control Non-repressible 4 (GCN4) in Arabidopsis for salt stress tolerance. GCN4 is an AAA+-ATPase (ATPases associated with diverse cellular activities). AAA+ proteins have diverse functions, such as assembly or disassembly of protein complexes, protein folding or unfolding, protein transport or degradation. It has been shown previously that when AtGCN4 overexpressed in Arabidopsis, it plays a significant role in host-pathogen interaction and control stomatal movement upon pathogen infection by degrading RIN4 - 14-3-3 proteins in PM H+-ATPase complex. Besides this GCN4 when overexpressed exhibit drought tolerance. In this study, we have investigated the previously developed overexpressed and silenced GCN4 Arabidopsis transgenic lines along with wild type plants for salt tolerance at different NaCl concentrations of 90mM, 120mM, and 150mM. So far, the studies indicate that the overexpressor lines were able to tolerate up to 150mm of NaCl as compared to the WT and silenced lines. Further studies are in progress to confirm our hypothesis that GCN4 improves the salt tolerance of transgenic plants. The obtained knowledge will help to investigate the common link in the mechanisms involved in various abiotic and biotic stresses.

Dodge, Reagan; Backman, Talia (Utah Valley University)
Faculty Advisor: Zahn, Geoffrey (Utah Valley University, Biology)

We assessed the ability of Pleurotus ostreatus, Oyster mushroom, to efficiently decompose waste products containing cellulose. As common pollutants are comprised of cellulose it is important to understand organisms that have cellulolytic enzymes such as P. ostreatus. The fungus P. ostreatus was introduced to the substrates of paper textiles, peanut shells, and livestock manure. After each treatment, the yield of mushroom production and remaining substrate was measured. As these substrates have been broken down during mushroom production they can be sold as inorganic fertilizer or compost. As well, Oyster mushrooms can be purchased and enjoyed in meals. Mushroom cultivation is a multi-billion dollar industry, and excessive wastes are a strain on the environment. By understanding what cellulase substrates produce the greatest yields we can eliminate waste all while increasing profits. Oh, and eating great mushrooms!

Stevens, Shaylee (Weber State University)
Faculty Advisor: Bialowas, Anne (Weber State University, Communications)

Since they were originally published, the Harry Potter books have become a cultural phenomenon that connects people from all around the world through its relatable characters and real-world themes. This paper examines the Harry Potter books through the lens of Cultural Studies to explain the connections in the books to the real world and how the theory can be applied to real life experiences. Previous literature related to Cultural Studies and the Harry Potter books provided a foundation for the analysis. The books were analyzed by applying three key concepts in Cultural Studies to various situations and themes in the stories. The three concepts used in the analysis were ideology, economic determinism, and hegemony. The concepts were used to explore the beliefs and values of different characters within the books, as well as the power dynamics and social structures of the wizarding world. These key themes and concepts highlight the parallels that can be drawn between the world of Harry Potter and the real world, and they show the ways in which Cultural Studies can apply to our daily lives.

Leatham, Dallin (Weber State University)
Faculty Advisor: Domek, Matthew (Weber State University, Microbiology); Oberg, Craig (Weber State University, Microbiology); Culumber, Michelle (Weber State University, Microbiology); Walker, Edward (Weber State University, Biochemistry)

Gouda cheese is known for its buttery flavor which is produced by the compound 2,3-butanedione or more commonly known as diacetyl. To measure the concentration of diacetyl in cheese a colorimetric test for vicinal diketones is used, known as the Voges and Proskauer (VP) method. This method involves using 5.0% a-naphthol and 0.4% creatine-KOH to develop a pink to red color. In the VP method the concentration of diacetyl is directly related to the absorbance. When this method was performed using known concentrations of Diacetyl in dH2O, a linear relationship was found to have a R2 value of .9571 confirming that the VP method is a viable method of testing for diacetyl concentration. It was proposed that by using a GC-MS to measure these concentrations a simpler test could be performed with similar or improved accuracy. Extracting of the diacetyl from Gouda cheese was done using a stomacher and a mixture of 50:50 acetone:dH2O. Before the concentration of cheese was measured dilutions of diacetyl concentrations were run in a 50:50 acetone:dH2O mixture at 0.1 uL/mL, 1 uL/mL, and 10 uL/mL. The concentrations of diacetyl that gouda cheese commonly contains are from 0.01 uL/mL to 0.1 uL/mL. It was found that the concentration of .1 uL/mL of diacetyl could not be distinguished from the background interference of the instrument. Currently it seems that using a GC-MS to determine diacetyl concentrations in gouda cheese might not be a practical method. Further research and a better understanding of the equipment could improve this method in order to obtain readable results.

Tooley, Sarah (Utah State University)
Faculty Advisor: Powell, Jake (College of Agriculture and Applied Sciences, Landscape Architecture and Environment Planning Department)

The dry, arid climate of Utah makes water a limited and precious resource. However, Utahans are the second largest per capita water users in the nation, with over 60% of that water used on outdoor landscapes. High landscape water use in the face of limited availability is a complex socio-cultural phenomenon reaching back to when pioneers first settled Utah. Settlers were from places with cultural perceptions of landscapes and climates much different then what they found when they arrived. Their desire to create a place that felt like home established a cultural landscape vernacular where Utahan's prefer a landscape more lush and green than we have water to support. With the projected increase in Utah's population, the traditional Utah landscape is quickly becoming unsustainable. As an Extension service I designed 5 landscapes following established water wise design principles which are: planning and design, plant selection and hydro zones along with practical turf areas. The purpose of these designs was to demonstrate that water wise designs can both be water efficient as well as functional, sustainable and aesthetically pleasing. The results of these planning and design efforts are currently being installed into residences in two Utah communities. I will share the design and implementation methods and results of these designs and visitor feedback data from one of the sites. This design research showcases a potential method others in Utah can use to make their landscapes more water wise and begin to redefine the vernacular landscape in Utah and the Intermountain West.

Tyler Hacking, Jonathan Wasden and Dr. Michael Stevens (Utah Valley University)
Faculty Advisor: Stevens, Michael (Science, Biology)

Abstract:

Plant growth can be affected by both genes and the environment. To test genetic effects on growth we used two varieties of lettuce. To test environmental effects on growth, we used four different water treatments. We were interested in the main effects of genes and the environment and also in their interaction. In other words, is the effect of water treatment different across two lettuce varieties? Two varieties of Lactuca sativa were cultivated in a growth chamber in pots using vermiculite as the growth medium. The two varieties were treated with four types of water: tap, tap-aerated, reverse osmosis, and reverse-osmosis-aerated throughout the experiment. Aeration was accomplished using both surface aeration (waterfall and vortex) and sub-surface aeration using submerged waterstones. All plants were fertilized using 8-15-36 (N-P-K) water-soluble lettuce fertilizer powder plus trace minerals by Greenway Biotech, Inc. The plants were harvested and dried to a constant weight for analysis of biomass. We observed varietal differences in growth with the Buttercrunch variety producing 145% more biomass than the Butterhead variety (p < 0.001). The water treatments affected growth, with the plants watered with tap water producing 31% more biomass than the plants watered with reverse-osmosis water (p < 0.001). The other two water treatments (tap-aerated and reverse-osmosis-aerated) were intermediate between the tap-watered plants and the reverse-osmosis-watered plants in terms of biomass. Finally, we observed a variety-by-environment interaction such that the Buttercrunch showed a marked response to water treatment whereas the Butterhead was only minimally affected by water treatment (p < 0.001). The Buttercrunch produced more biomass than the Butterhead because of its shorter life cycle. By the end of the experiment, the Buttercrunch plants were already bolting and producing flowers. This could also explain why the Buttercrunch was more responsive to differences in water treatments.

West, Bethany (Weber State University)
Faculty Advisor: Van Gilder, Bobbi (Weber State University: Lindquist College of Arts and Humanities, Communications)

Gendered language, and the way it dictates societal norms, has been a topic of interest for feminist scholars since the 1970's. However, gendered language and its connection with rape culture has yet to be explored, presenting a substantial gap in research. I researched the ways that language may influence and perpetuate rape culture by analyzing #MeToo stories, which are victim's accounts of being sexually assaulted. I focused on the specific language victims used to describe their sexual assault, such as sexist or gendered language. By analyzing the language used in sexual assault victims' stories, I drew connections between rape culture and gendered language. Many female victims emphasized that power differentials between them and their male attacker, such as being viewed as subordinate in their relationship with their attacker, played a significant role in their abuse. Other victims emphasized that social discourse normalized rape for women, and placed blame on victims rather than attackers. Victims also indicated that social norms regarding sexual assault asserted that only heterosexual women were victimized, silencing marginalized victims such as men and members of the LGBTQ community. Socially implicated stereotypes regarding gender and rape were found to create situations in which assault victims were silenced and further abused. By understanding and challenging the discourse regarding language, gender norms, and the roles that they play in sexual assault, society may be able to combat rape culture and those who are at risk for victimization.

Dickamore, Erik (Utah State University)
Faculty Advisor: Colver, Mitchell

Becoming integrated into the social sphere of an institution is a vital step for new and continuing students in a university setting. There is a body of studies that have examined the impact of different aspects of the student experience on persistence. Many of them center on how becoming integrated into the social sphere of an institution is impactful on persistence. While social integration is considered essential for success, few studies have rigorously considered if social integration through student involvement and leadership is associated with persistence toward graduation. This research is intended to estimate causal links between treatments and expected student outcomes. More specifically, this research will highlight the estimated causal impacts on persistence to the next term for students who participate in student involvement. Historically, there has been a roadblock of understanding the effect that leadership and student involvement programs have on students due to self-selection bias. This research uses Prediction Based Propensity Score Matching (PPSM), a quasi-experimental method, to control for baseline variability and account for self-selection bias. Results highlight an estimated causal link to persistence and being involved in leadership positions at a 4-year institution in the mountain west of the United States.

Mendiola, Diego (Utah State University)
Faculty Advisor: Bullock, Cathy (College of Humanities and Social Sciences, Journalism and Communication Department)

To understand journalism and communication is to know who we are as human beings and what our journey through time means to us; through the systematically detailed logging of our daily experiences we witness the creation of our history; living out moral, ideological and cultural ideals through the word. It is to see ourselves reflected, to see our time, our values, our hypocrisy, our psychology reflected onto the depths of space and time.

For this reason, I believe it is imperative that we get a grip on the methods we use to communicate that historical dialogue we call journalism effectively, providing the least amount of trouble to the living generations of people that inhabit the earth. The promise of an educated, autonomous population is the one that keeps journalism alive and relevant. I have found a set of solutions that are to be discussed among professionals and academics alike, including the reforming of communicative structures and a more conversational relationship with the audience about the larger direction of media.

Black, Brent; Peterson, Savannah (Utah State University)
Faculty Advisor: Black, Brent (College of Agriculture and Applied Science; Plants, Soils, and Climate Department)

Utah has 1,200 acres of commercial peaches (15th largest peach producing state) making it the second most important fruit crop in the state. However, Utah soils are alkaline, and many trees grown on alkaline soils struggle with iron chlorosis. Peaches and other fruit trees are a combination of the desired variety (scion) grafted onto a rootstock. Some peach rootstocks may be better adapted to Utah's alkaline soils. An orchard comparing 12 peach rootstocks was planted in Utah County in 2007 and evaluated in the 2019 season for survival, tree size and iron chlorosis (leaf chlorophyll content, an indicator of alkaline soil tolerance).The scion for all trees was O'Henry. Most rootstocks had good survival after 13 years (>80%). Empyrean 2 and Julior had the poorest survival (< 50%). The largest trees were on Monegro, Empyrean 1 and Nickels rootstocks, and the smallest were on Lovell, Julior and Krymsk 86. Leaf chlorophyll was lowest (most chlorotic) for Lovell, Julior and Empyrean 1. Selecting the best adapted rootstock is essential for an orchard to be successful and profitable. These results will be useful for Utah growers to select trees best adapted to their conditions.

Vasquez, Cesar; Dockstader, Patrick; Havertz, Brett; Phillips, Justin (Dixie State University)
Faculty Advisor: Chellamuthu, Vinodh (Dixie State University, Mathematics)

For any business, understanding the customer’s behavior is vital to maximizing income and minimizing costs. Our work aims to create an algorithm that analyzes the historical data from the customers and determines the target customers in an optimal way. We take on a data set from a transmission shop in California and seek out which factors produce higher potential for client value. We created a mathematical model that classifies the clients as low, medium, or high potential using this historical data. Furthermore, we demonstrated the model utility using the transmission shop’s data to compute the correlation of paying customers and customer history. The correlations are then used to create a conditional probability distribution which served to predict an expected rating score. Moreover, our results are validated by comparing the predicted ratings with the actual ratings in varying train and test cases from the data set. Our results show that the proposed algorithm is fast, simple, and intuitive, which could be utilized by the transmission shop in the future.

Waldron, Connor; Moley, Laura; Isom, S. Clay (Utah State University)
Faculty Advisor: Isom, S. Clay (College of Agriculture and Applied Sciences; Animal, Dairy, and Veterinary Sciences Department)

In vitro maturation (IVM) is a process that immature oocytes undergo before in vitro fertilization is performed. During IVM, immature oocytes are extracted through aspiration from follicles and receive the necessary hormones to resume meiosis in culture. Success rate of IVM is significantly lower compared to in vivo maturation of oocytes. Oocytes selected for IVM are extracted from small ovarian follicles (1-3mm), which are 4-6 weeks away from complete maturation and subsequent ovulation. During that period, the DNA of the oocyte is undergoing the process of DNA methylation, the addition of a methyl group to cytosines within a CpG context. DNA methylation is an epigenetic change that causes modification to gene expression where methylated gene promoters turn off gene expression. Proper oocyte gene expression is very important in the development of a healthy embryo. Incomplete methylation of aspirated oocytes may be another factor contributing to the low success rates of IVM. We hypothesize that there is a difference in the amount of methylation between oocytes from small ovarian follicles that are further from natural ovulation and oocytes from large ovarian follicles which are developmentally closer to ovulation, with higher levels of methylation in oocytes from large ovarian follicles. To test our hypothesis, large porcine ovarian follicles (7mm and larger) and small porcine ovarian follicles (1-3mm) will be aspirated for ovaries. The oocytes will be stained the two stains, one to visualize the nucleus of the oocyte indicating the presence of DNA and the other to visualize DNA methylation. Fluorescent images will be taken of the oocytes, and the small and large follicle oocyte groups will be compared for genome wide methylation levels.

Park, Yeram; Lin, Jade; Ross, Micah; Stark, Michael; (Brigham Young University)
Faculty Advisor: Stark, Michael (Life Sciences, Physiological and Developmental Biology); Hansen, Marc (Life Sciences, Physiological and Developmental Biology)

Neural tube defects (NTDs), which result from failure to close the neural tube during embryonic development, are one of the most widespread and common congenital malformations. Variance in these malformations can range from anencephaly (failure of the neural tube to close on the cranial end) to spina bifida (failure of closure on the posterior/dorsal end). Over the years, scientists have explored this field and have found different environmental factors that may attribute to the likelihood of NTDs. Some of these include fumonisin, valproic acid and more recently discovered, ceramide. To help counter NTDs, studies have shown that folic acid supplementation given to pregnant women has reduced the risk of NTDs and this has become a recommended suggestion by doctors. With its known preventative effects, this study aims to determine whether the preventative effects of folic acid can counter the harmful effects of fumonisin, valproic acid, or ceramide.