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2020 Abstracts

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General Control Non repressible 4 (GCN4) improves salt tolerance in Arabidopsis transgenic plants

December 30, 0020 12:00 AM
Kaundal, Amita; Hansen, Nathaniel; Ganesh, Jyothsna (Utah State University)
Faculty Advisor: Kaundal, Amita (College of Agriculture and Applied Sciences; Plants, Soils, and Climate Department)

Plants are sessile and bound to their origin so they cannot move to defend themselves against adverse environmental conditions. Soil salinity is one of such environmental stress, which limits the growth and development of plants. Salt stress directly affects crop production. It has been predicted that by 2050, about 50% of arable land will be affected by salinity. About 23% of the worldwide farmland is affected by soil salinity and the crop losses due to salinity are estimated to be tens of billion dollars per year. On top of that, an increase in the world population, the demand for food production also increases. Thus, feeding the growing population under adverse conditions is a challenge. This challenge demands to create more resilient crops to adverse conditions and to feed the growing population. In this study we are investigating General Control Non-repressible 4 (GCN4) in Arabidopsis for salt stress tolerance. GCN4 is an AAA+-ATPase (ATPases associated with diverse cellular activities). AAA+ proteins have diverse functions, such as assembly or disassembly of protein complexes, protein folding or unfolding, protein transport or degradation. It has been shown previously that when AtGCN4 overexpressed in Arabidopsis, it plays a significant role in host-pathogen interaction and control stomatal movement upon pathogen infection by degrading RIN4 - 14-3-3 proteins in PM H+-ATPase complex. Besides this GCN4 when overexpressed exhibit drought tolerance. In this study, we have investigated the previously developed overexpressed and silenced GCN4 Arabidopsis transgenic lines along with wild type plants for salt tolerance at different NaCl concentrations of 90mM, 120mM, and 150mM. So far, the studies indicate that the overexpressor lines were able to tolerate up to 150mm of NaCl as compared to the WT and silenced lines. Further studies are in progress to confirm our hypothesis that GCN4 improves the salt tolerance of transgenic plants. The obtained knowledge will help to investigate the common link in the mechanisms involved in various abiotic and biotic stresses.
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The Effects of Housing on Student Persistence

December 30, 0020 12:00 AM
Colver, Mitchell; Hagman, Amanda (Utah State University)
Faculty Advisor: Colver, Mitchell; Hagman, Amanda

Making the choice of where to live while in college is frequently acknowledged as one of the most important decisions an undergraduate student makes. Housing decisions influence students' access to campus resources and social integration, elements thought to be key indicators of their progression towards graduation. Interestingly, however, the association between living on-campus and persistence has not been considered thoroughly in the literature. While many studies leverage survey data and retention rates to make direct comparisons between on-campus and off-campus groups, most are unable to account for self-selection bias, i.e. that students who live on-campus may be qualitatively different from students who chose to live off-campus. The present study overcame this challenge by utilizing a matching technique called Prediction-based Propensity Score Matching (PPSM). Using this theoretically-driven and methodologically robust technique, researchers were able to account for self-selection bias and estimate the impact of on-campus living on student persistence. After matching, researchers estimated that students living on-campus experience a 1.19% lift (CI: 0.55% to 1.83%) in persistence. In other words, the model suggests that 46 students (CI: 21 to 71) remain enrolled at the institution simply because they live on-campus. This conclusion indicates that living in university housing is not only important because it provides students with campus proximity and social activities, but ultimately because it helps them persist towards graduation.
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Aggies Go Green: Sustainable Transportation

December 30, 0020 12:00 AM
Kaytriauna Flint, Allison Fishler, Mosese Manu, and Justus Te'i (Utah State University)
Faculty Advisor: Soyer, Mehmet (College of Humanities and Social Sciences; Sociology, Social Work and Anthropology Department)

Here at Utah State, there are many resources available for students, staff, faculty, and administration to travel to and from campus in eco-friendly ways. While there are many resources readily available, they are not well known across campus. Our initiative is to bring awareness to these hard-working groups and generous programs while incentivizing those who participate in our social media campaign where they will submit creative photos of their sustainable transportation to and from campus. Not only will it be extremely beneficial for these groups and programs across campus to get well-deserved recognition through this initiative, but it will give Aggies the greater opportunity to Go Green and travel to and from campus in an eco-friendly way!
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Coding and Mathematics Skills: Case Studies

December 30, 0020 12:00 AM
Evans, Hannah; Peterson, Rebecca (Utah State University)
Faculty Advisor: Shumway, Jessica (Emma Eccles Jones College of Education & Human Services, School of Teacher Education and Leadership); Clarke-Midura, Jody (Emma Eccles Jones College of Education & Human Services, Instructional Teachnology and Learning Sciences Department); Lee, Victor (Emma Eccles Jones College of Education & Human Services, Instruction Technology and Learning Sciences Department); Silvis, Deborah (Emma Eccles Jones College of Education & Human Services, Instruction Technology and Learning Sciences Department)

The push for computer science integration in kindergarten classrooms is a surfacing topic in public education in the United States. One approach to this is to integrate computer science with mathematics. However, there is minimal research on computer science and computational thinking as it relates to mathematics in early childhood education. In order to address this need, we are part of a larger research project that is studying the integration of computer science and mathematics in kindergarten classrooms. Specifically, we are exploring computational thinking (CT) elements that we see students engaging in (e.g., algorithmic thinking, debugging, and decomposition) while at the same time exploring mathematics skills that emerge (e.g., measurement, spatial reasoning, and pattern recognition). We study these skills and elements as we teach formal coding instruction using play-based, screen free coding robots. In this presentation, we will present early results of this integration in local kindergarten classrooms. Our main research question is: What CT and mathematics skills and strategies emerge as a result of this formal coding instruction?

Our sample consists of 16 kindergarten students. Groups of 3-4 students participated in six 30-minute coding lessons, followed by an assessment interview. Our data set is made up of video data from both the coding instruction and the assessment interviews, as well as field notes and assessment score sheets. We will present descriptive statistics regarding these assessments, as well as in-depth case studies of 2 students (one student who scored high on their post-test, and one who scored low). We will present data from the videos of their individual experiences as they engaged in formal instruction, as well as data regarding their assessments, in order to answer our research question and analyze what CT and mathematics skills and strategies emerged as a result of their formal coding instruction.
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Examining the Influence of Science-Infused Literacy Instruction on Reading Motivation and Quality of Informational Text

December 30, 0020 12:00 AM
Judd, Emma J.; Clark, Sarah K. (Brigham Young University)
Faculty Advisor: Clark, Sarah (Education, Teacher Education)

The study examined the integration of science into literacy instruction for the early grades. The study specifically explored how science-infused literacy instruction influenced the quality of compare and contrast science informational text produced by second graders, compared to literacy instruction alone. Additionally, the motivation of the students to read was examined in connection to the quality of the informational text they produced. The participants were second-graders (N = 72) between the ages of 7 – 9 and were enrolled in the first quarter of the school year at a Title 1 school. Seventy-three percent of these students were White, 22% were Hispanic, 2% were Asian, and 1% were Black, with 63% of the students eligible for free or reduced lunch. The treatment group (N = 35) received science-infused literacy instruction and the control group (N = 37) received their regular literacy instruction. Two instruments for measurement were used: a motivation survey which measured the students’ motivation to read, and a weighted writing rubric with an emphasis on signal words, content-specific vocabulary words, and word count, which was used to score the informational text writing samples produced by the students. The students took the reading motivation survey and answered the same informational text writing prompt before and after the instruction. The instruction for compare and contrast writing took place over the course of a four-week unit, and consisted of three 30-minute lessons weekly. The research was designed as quasi-experimental using a paired samples t-test with follow-up effect size measures. The results demonstrate that students in the treatment group produced significantly higher reading motivation scores but wrote lower-quality text, while students in the control group produced lower motivation scores but wrote higher-quality informational text. Implications from this study will be provided for educators and literacy instructional practices, and for researchers and future studies.
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Construction of candidate vectors for correction of the intestinal CFTR gene expression in Cystic Fibrosis sheep fetal fibroblast cells

December 30, 0020 12:00 AM
Perisse, Iuri Viotti; Fan, Zhiqian; Wang, Zhongde; Harris, Ann; White, Kenneth L.; Polejaeve, Irina A. (Utah State University)
Faculty Advisor: PoleJaeva, Irina (College of Agriculture and Applied Sciences; Animal, Dairy, and Veterinary Sciences Department)

Cystic Fibrosis (CF) is a recessive human genetic disease that is caused by mutations in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene. This gene is responsible to transport Cl- and HCO3- anions in epithelial cells. Previously, we generated CFTR-/- lambs using CRISPR/Cas9 and SCNT techniques. The CFTR-/- lambs display many features similar to human CF disease, including meconium ileus (MI), pancreatic fibrosis, portal fibrosis and biliary hyperplasia, small gallbladder, and absence of vas deferens. In CF patients, MI affects only 15-20% of human babies, whereas it was observed in 100% of newborn CFTR-/- lambs and was the primary cause of death. We here hypothesized that the transgenic expression of the ovine CFTR cDNA under regulation of an intestinal-specific expression promoter would promote the correction of MI in CFTR-/- sheep. In this study, we are constructing three potential vectors with different promoters to be evaluated prior to the generation of transgenic animals. Rat intestinal Fatty Acid Binding Protein (iFABP), rat liver Fatty Acid Binding Protein (LFABP), and Villin1 promoters have already been characterized and successfully used for intestinal-specific expression. After digestion and ligation cloning, the three constructs will be sequenced to confirm the presence of all segments (promoter, cDNA, and vector) in the correct orientation. Subsequently, we plan to evaluate the transient gene expression of the constructs in CaCo-2 cells to ensure they are fully functional. Therefore, we will construct the pcDNA3.1>promoter>CFTR expression vector in order to generate intestine-CFTR transgenic CFTR-/- sheep.
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Funding Community College Child Care Services: How the Child Care Access Means Parents in School (CCAMPIS) Funding Influences Institutional Policy and Practice

December 30, 0020 12:00 AM
Avondet, Callie (University of Utah)
Faculty Advisor: Taylor, Jason (College of Education; Education, Leadership & Policy)

A shocking 33% of student parents graduate from college (Institute for Women's Policy Research, 2014). Part of this low graduation rate has been attributed to scheduling difficulties: the hours classes are offered, planning group-project meetings, and out of class requirements making it difficult to coordinate childcare (Kensinger and Minnick, 2018). Child Care Access Means Parents in School (CCAMPIS) is a federal program that provides money for childcare services to qualifying students at institutions whose students receive a total of $350,000 or more in federal Pell grant funding (Baskerville, 2013) . In fiscal year (FY) 2018 the overall amount of funding allocated to CCAMPIS and the number of schools getting this government grant nearly doubled. This provided CCAMPIS funding to many colleges that did not previously have it (Department of Education, n.d.). This project interviews the child care director from one randomly selected community college from each of the 9 US Census districts (excluding DC and Puerto Rico) to understand how CCAMPIS funding for FY 2018 changed institutional policy, practice, and programs related to child care in community colleges that did not receive CCAMPIS funding in FY 2017. Although data is still being collected, preliminary data suggests that CCAMPIS allows institutions to mold their childcare programs to the needs of their students through various projects. It also frees up more funding to help students who would still struggle financially to get access to childcare but do not meet the formal requirements for CCAMPIS money.
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Determination of Diacetyl Concentration in Gouda Cheese

December 30, 0020 12:00 AM
Leatham, Dallin (Weber State University)
Faculty Advisor: Domek, Matthew (Weber State University, Microbiology); Oberg, Craig (Weber State University, Microbiology); Culumber, Michelle (Weber State University, Microbiology); Walker, Edward (Weber State University, Biochemistry)

Gouda cheese is known for its buttery flavor which is produced by the compound 2,3-butanedione or more commonly known as diacetyl. To measure the concentration of diacetyl in cheese a colorimetric test for vicinal diketones is used, known as the Voges and Proskauer (VP) method. This method involves using 5.0% a-naphthol and 0.4% creatine-KOH to develop a pink to red color. In the VP method the concentration of diacetyl is directly related to the absorbance. When this method was performed using known concentrations of Diacetyl in dH2O, a linear relationship was found to have a R2 value of .9571 confirming that the VP method is a viable method of testing for diacetyl concentration. It was proposed that by using a GC-MS to measure these concentrations a simpler test could be performed with similar or improved accuracy. Extracting of the diacetyl from Gouda cheese was done using a stomacher and a mixture of 50:50 acetone:dH2O. Before the concentration of cheese was measured dilutions of diacetyl concentrations were run in a 50:50 acetone:dH2O mixture at 0.1 uL/mL, 1 uL/mL, and 10 uL/mL. The concentrations of diacetyl that gouda cheese commonly contains are from 0.01 uL/mL to 0.1 uL/mL. It was found that the concentration of .1 uL/mL of diacetyl could not be distinguished from the background interference of the instrument. Currently it seems that using a GC-MS to determine diacetyl concentrations in gouda cheese might not be a practical method. Further research and a better understanding of the equipment could improve this method in order to obtain readable results.
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Amaebae in warming soils : The interactive effects of protozoan predation pressure and environmental factors on Nitrogen Cycling in soils under warming conditions

December 30, 0020 12:00 AM
Dodge, Reagan; Backman, Talia (Utah Valley University)
Faculty Advisor: Zahn, Geoffrey (Utah Valley University, Biology)

We assessed the ability of Pleurotus ostreatus, Oyster mushroom, to efficiently decompose waste products containing cellulose. As common pollutants are comprised of cellulose it is important to understand organisms that have cellulolytic enzymes such as P. ostreatus. The fungus P. ostreatus was introduced to the substrates of paper textiles, peanut shells, and livestock manure. After each treatment, the yield of mushroom production and remaining substrate was measured. As these substrates have been broken down during mushroom production they can be sold as inorganic fertilizer or compost. As well, Oyster mushrooms can be purchased and enjoyed in meals. Mushroom cultivation is a multi-billion dollar industry, and excessive wastes are a strain on the environment. By understanding what cellulase substrates produce the greatest yields we can eliminate waste all while increasing profits. Oh, and eating great mushrooms!
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Comparing Peach Rootstocks for Adaptability in Utah Soils

December 30, 0020 12:00 AM
Black, Brent; Peterson, Savannah (Utah State University)
Faculty Advisor: Black, Brent (College of Agriculture and Applied Science; Plants, Soils, and Climate Department)

Utah has 1,200 acres of commercial peaches (15th largest peach producing state) making it the second most important fruit crop in the state. However, Utah soils are alkaline, and many trees grown on alkaline soils struggle with iron chlorosis. Peaches and other fruit trees are a combination of the desired variety (scion) grafted onto a rootstock. Some peach rootstocks may be better adapted to Utah's alkaline soils. An orchard comparing 12 peach rootstocks was planted in Utah County in 2007 and evaluated in the 2019 season for survival, tree size and iron chlorosis (leaf chlorophyll content, an indicator of alkaline soil tolerance).The scion for all trees was O'Henry. Most rootstocks had good survival after 13 years (>80%). Empyrean 2 and Julior had the poorest survival (< 50%). The largest trees were on Monegro, Empyrean 1 and Nickels rootstocks, and the smallest were on Lovell, Julior and Krymsk 86. Leaf chlorophyll was lowest (most chlorotic) for Lovell, Julior and Empyrean 1. Selecting the best adapted rootstock is essential for an orchard to be successful and profitable. These results will be useful for Utah growers to select trees best adapted to their conditions.
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Far from Grub Street: Satire in George Knapton's Portraits of the Society of Dilettanti

December 30, 0020 12:00 AM
Noorda, Meredith (Brigham Young University)
Faculty Advisor: Belnap, Heather (Humanities, Comparative Arts and Letters); Swensen, James (Humanities, Comparative Arts and Letters)

In 1740 the Society of Dilettanti, an 18th century group of English gentlemen aiming to encourage an appreciation for the antiquities they had seen on their Grand Tours, decided to commission from George Knapton portraits of all their members. In the typical artistic vein of the early Dilettanti, the portraits, featuring many of the members in costume, exhibit a milieu where the erudite meets the comical to the outright lewd, a reflection of one of the Dilettanti's mottos of seria ludo, or "serious things done in a playful spirit." Within the wider context of London public life, these are also clear examples of the influence of the masquerade, and for a group closely aligned with the Italian Grand Tour, and thought to have been initially conceived in Venice, the appeal of those references is clear and the Dilettanti's use of them has been commented on in scholarship. However, the use of costume in George Knapton's portraits can reveal more than a simple love of Continental entertainments. Aligning oneself with the Continent and the East, as the sitters do in Knapton's works, was a subversive choice in relation to the normative British culture of the mid-18th century, in which the encroachment of Continental entertainments and fashion, among other things, was frequently feared. These portraits must be read with an acknowledgement of the ways in which the masquerade and the Italianate was seen in 18th century England, where it was not an accepted form of entertainment as on the Continent, but a controversial pastime. In turn the portraits can reveal where the Dilettanti situated themselves, and how they wanted to be represented�as internationally inclined participators in these foreign entertainments, boldly partaking in its vices, championing its creative possibilities in the self-fashioning of an individual.
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A Comparative Study of Detection Methods: Early Optical Telescopes and Gravitational Wave Detectors

December 30, 0020 12:00 AM
Maria Stokes (University of Utah)
Faculty Advisor: Burnett, Brandon (Weber State University, Chemistry)

This paper considers the relationship between new technologies and the history of astronomy. Using a comparative framework, I show some of the ways in which new technological introductions alter scientific practice. I argue that this dynamic is a historical pattern. To make this case, I juxtapose two astrophysical developments: the invention and early uses of optical telescopes in the early seventeenth century, most famously by Galileo, and the introduction of gravitational wave detectors beginning with the Laser Interferometer Gravitational-Wave Observatory (LIGO). The former has been heavily examined by historians of science; the latter is almost exclusively of interest to astronomers and physicists. In constructing this comparison, I examine primary sources such as Galileo's Discourses and Mathematical Demonstrations Related to Two New Sciences and consult other commentaries on seventeenth-century astronomy, particularly remarking on the optics used in the Galilean telescope. I then provide a survey of gravitational wave astronomy. This comparative study evidences the importance of both empirical data and networks in the development of science. Such a conclusion is significant as it carries implications for the relationship between scientific and non-scientific communities.
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Folklore: As it is Perceived by USU English Majors in Comparison to USU Non-English Majors

December 30, 0020 12:00 AM
Christensen, Nikki (Utah State University)
Faculty Advisor: Kinkead, Joyce (College of Humanities and Social Sciences, English Department)

Folklore is one of the hardest subjects to define for students and professors alike. To this day, folklorists still dispute its accurate definition. The most popular contemporary definition is, "artistic communication in small groups"(Dan Ben-Amos). This research is to discover if English majors are more educated about folklore than non-English majors at Utah State University. Often, students will be immersed in folklore in their daily lives and never know it. This research is to answer how relevant folklore still is in today's society.
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Are You Smarter Than an English Major? How Grammar Checkers are Perceived by Upper Division English Majors

December 30, 0020 12:00 AM
Roberts, Katie (Utah State University)
Faculty Advisor: Kinkead, Joyce (College of Humanities and Social Sciences, English Department)

The internet has provided students with countless opportunities for success and learning, online grammar checkers and blogs playing a significant role for many in their pursuit of education. Grammar blogs offer tips, tricks, and examples to help people learn grammar, while grammar checker websites, such as Grammarly, use AI technology to automatically review pieces of writing, sometimes offering plagiarism checks and citation suggestions with a paid subscription. While these websites proclaim to make all the difference in students' writing, are English majors finding these helpful? This research seeks to understand what upper-division English majors think of online grammar checkers.
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Did She have to Die? An Examination of Hero and Ophelia in the Context of Shared Plot Points

December 30, 0020 12:00 AM
Witham, Arianna (Dixie State University)
Faculty Advisor: Pilkington, Olga (Dixie State University, Applied Sociology)

Two of Shakespeare's plays Much Ado About Nothing and Hamlet have plots that are largely dependent on the death of a female character. Looking at the deaths of Hero and Ophelia though the lens of feminist literary analysis shows that these deaths are transformative points. On the surface, these deaths are very different: Ophelia stays dead while Hero's death is only a ruse. However, the deaths of Hero and Ophelia transition both of them from persons to objects. In the case of Ophelia, after death all that is left is a corpse, and Hamlet and Laertes' altercation in Ophelia's grave on top of her body contradicts their sorrowful proclamations just before. After Hero re-enters society, admittedly as someone else, the only thing about her new identity that seems to matter is her physical similarity to the Hero most believe to still be dead. Considering genre prompts another comparison between these deaths. In Much Ado About Nothing, Hero's death prompts Beatrice and Benedict's confessions of love, and her return to society allows the weddings and celebration that finish the play and mark it as a comedy to happen. Tragedies are marked by the death and destruction of the characters, and Hamlet is no exception. Ophelia's death is a catalyst for events that lead to the deaths of the other characters. If she had been revived, then there would be no funeral, and the emotional stakes at the end of the play would not be as high. The deaths of both Ophelia and Hero serve to drive the plots of these plays, but at the cost of the personhood of each.
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Adapting Origami Principles to Improve Performance of Disposable Incontinence Products

December 30, 0020 12:00 AM
Beatson, Bridget; Bolanos, Diana; Jackson, Corinne (Brigham Young University)
Faculty Advisor: Vargis, Elizabeth (College of Engineering, Biological Engineering Department)

Origami, traditionally known as the art of paper folding, is not limited solely to paper media. The concepts used in folding paper can also apply to more pliable materials such as fabric. As with paper, different fabric-based origami designs exhibit properties such as shape compliance, increased surface area per unit volume, and selective stiffness. This study explores selected fold patterns in various incontinence product fabrics, aiming to increase fluid wicking performance and thus reduce sag due to saturation. The most suitable materials from various adult incontinence product brands were tested then utilized to develop new concepts for integration into an innovative and revolutionizing product. For the liquid dispersion layer, the concept of pleated fabric was incorporated into the design, mimicking the origami characteristics of peaks and valleys. Tests were performed on suitable materials to measure the spread of fluid in the modified layer as would be actuated by human urination. Final results from this testing indicated significantly broader dispersion of the synthetic urine utilized for testing as compared to that of the unmodified materials. When incorporated into a final product, this would allow for larger distribution of the fluid, thus increasing the product's holding capacity and enabling a more even distribution of the weight of the fluid, helping to reduce sag. This result could greatly increase the comfort and functionality of adult incontinence products.
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Assessing the Validity of The Test of Early Written Language (3rd Edition)

December 30, 0020 12:00 AM
Anderson, Bethany; Ward, Hannah; Froerer, Cecily (Utah State University)
Faculty Advisor: Gillam, Sandra (Emma Eccles Jones College of Education & Human Services, Communicative Disorders and Deaf Education Department); Gillam, Ron (Emma Eccles Jones College of Education & Human Services, Communicative Disorders and Deaf Education Department)

Children with language disorders face difficulties in producing organized and complex narratives. Many different assessments have been designed to allow clinicians to evaluate a child's narrative ability. The Test of Early Written Language - 3 (TEWL) is a formal test to assess the early written narrative abilities of children. Valid and reliable tests of the written language skills of young elementary age children are needed for assessing the outcomes of narrative interventions for children who are at-risk for language and literacy impairments.

The purpose of this study is to determine if the TEWL is a valid measure of narrative language in written stories by children who are at-risk for language and literacy problems.

Narrative samples from 189 children ages 6-11 were gathered as part of a larger study. Narrative samples were transcribed and scored using the Monitoring Indicators of Scholarly Language (MISL) rubric that assesses language microstructure and macrostructure. Exploratory and confirmatory factor analyses were computed to determine the extent to which the total raw scores on the TEWL were related to MISL microstructure, macrostructure, and total scores. Item analyses were conducted to determine whether a subset of items on the TEWL differentiate between writing mechanics and written language. Results are critical for using the TEWL as a valid outcome measure in studies of interventions for oral and written language comprehension and production.
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Barbara Kingsolver's Flight Behavior: An Examination of Climate Change Discourse

December 30, 0020 12:00 AM
Truman, Jorden (Dixie State University)
Faculty Advisor: Pilkington, Olga (Dixie State University, English)

This paper uses literary analysis and theoretical framework of Regionalism to examine Barbara Kingsolver's novel Flight Behavior. Flight Behavior features Dellarobia, an inhabitant of a poverty-stricken Appalachian town, who discovered that a hill in her backyard became home to millions of wintering Monarch Butterflies. This abnormal flight behavior of the Monarch Butterflies for many in Dellarobia's community is seen as a gift from God, but for the outsiders, it is an ominous sign of climate change. The results of my analysis show that Kingsolver, although praised for her other novels set in the Appalachias, fails to address what Regionalist novels are criticized for: preventing the reader from taking sides with the educated master narrator against the abnormal or aberrant natives. Kingsolver's pursuit of the moral imperative comes at the cost of disparaging and humiliating the communities that makeup Appalachia. The ramifications of Flight Behavior are troubling because this "if you are not with us, you are against us" style of narration is prominent in climate change activist's discourse: creating enemies instead of allies.
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