2020 Abstracts

Sommers, Taryn; Veon, Raymond (Utah State University)
Faculty Advisor: Veon, Raymond (Caine College of the Arts, Art and Design Department)

A literature search of research and academic journals suggests that there are few critical examinations of LDS visual culture in visual culture research. The purpose of this exploratory study is to examine the types of gender roles portrayed in contemporary LDS visual culture, the visual codes used to represent these gender roles, and the degree to which the representations of gender used in LDS visual culture align with findings in related visual culture studies. The result of this study will be a description of the current visual strategies used by the LDS church to represent gender roles, a thematic analysis of these visual strategies, and suggestions for future research into the ways that the LDS church visually defines gender in relation to existing trends and themes in visual culture research. The scope will include: “Mormon Ads,” images used in the LDS magazine, “Ensign,” and conference memes used during the years 2016-2019. This will contribute to the field of visual culture and gender studies by examining the visual codes used by the LDS church to portray the role and status of males and females as represented in current LDS visual culture, determining the strategies used in these representations, and discussing the potential research implications of these findings in light of themes in visual culture gender studies. Some research questions are: What visual codes are used in the representation of males, females, and mixed-sex groups in current LDS visual culture? What types of roles and status do these visual codes convey pertaining to the representation of males, females, and mixed-sex groups in current LDS visual culture? What extent do the results of investigating the above research questions align with other research findings in gender and visual culture studies? The methods used in this research will be 1) an inventory of discrete visual codes (e.g. color use, affect/expression, clothing/dress, setting, etc.) used in the LDS visual culture samples, and 2) a separate thematic analysis of the sample will also be conducted. The results of both methods will be compared, analyzed, and discussed in light of current trends in visual culture gender research. While the results of this research are expected to demonstrate that LDS visual culture will emphasize and reinforce traditional gender roles of females as mothers and homemakers and males as leaders and providers, it is hoped that this study will reveal strategies related to gender role representation unique to LDS culture.

Marissa Devey (Utah State University)
Faculty Advisors: Vigneault, Marissa (Caine College of the Arts, Art and Design Department); Winward, Robert (Caine College of the Arts, Art and Design Department)

To those of us who are privileged enough to pad ourselves from the immediate consequences of global climate change, its reality remains an abstract and intangible problem. In the tropical cloud forests of Costa Rica, however, the effects of global warming are readily evident. My research aims to provide a narrative of climate change in the uniquely diverse and delicate cloud forest ecosystem, where I work in partnership with Ph.D. ecology student Jessica Murray. While Jessica uses sensors and datasets to quantify an intricate network of plants, animals, and microorganisms, my intent is to help non-scientists to visualize the implications of Jessica’s findings. My purpose is not to simplify or re-tell Jessica’s research, but to help the viewer connect with unfamiliar, abstract ideas in a visceral, internal way. I leverage paint, poetry, infographics, and abstraction to construct a metaphor for climate ecology: the human body. Working within this metaphor allows me to channel the viewer’s own physical, bodily experiences into an intimate portrait of ecological systems and their vulnerability to climate change. My goal is for viewers to become more conscious of their own ecology-- more aware of their dependence on the environment and more compassionate towards its components.

Holdaway, Bailey; Emily, Martin; Kettenring, Karin (Utah State University)
Faculty Advisor: Kettenring, Karin (S.J. & Jessie E. Quinney College of Natural Resources, Watershed Sciences Department);

Seeds are the primary revegetation method for Great Salt Lake wetlands, however, the density and the priority timing to sow seeds are not clear to wetland managers due to a lack of Great Salt Lake specific revegetation research. Having too low a native seed sowing density could allow unwanted species like the non-native invasive plant Phragmites to reinvade. Too high of a density and density-dependent mortality of sown native seeds could occur, resulting in wasted seeds and unneeded costs for resource-limited managers. In addition, the priority timing (i.e., the relative time and order that seeds are sown) of sowing is also vital for revegetation to favor natives over unwanted invasives. Therefore, our research goal was to determine the optimal seed sowing density and timing priority for reestablishing Great Salt Lake native wetland plant communities. We conducted an outdoor mesocosm experiment with two native sowing densities (3 and 5x the standard sowing density in the region) and three native seed mix sowing timings (4, 2, or 0 weeks prior to sowing Phragmites seeds). We determined the cover of the native plant community and Phragmites at the end of the growing season across the 6 treatment combinations. We found the greatest reduction in Phragmites cover when the native seed mix was sown 4 weeks prior to Phragmites, particularly at the higher native sowing density. A 2-week priority effect did not significantly benefit native species over Phragmites. These results suggest that native seed mixes in Great Salt Lake wetland restorations need to sown much earlier in the summer growing season than when Phragmites seeds germinate and at a very high density to reduce Phragmites cover overall. Though, managers may need to greatly reduce Phragmites seed densities in the seed bank and in the vicinity of restoration sites before revegetation efforts begin.

Hansen, Wade; Stockard, Alyssa; Anderson, Elizabeth; Yorgason, Jordan; Sudweeks, Sterling; Wu, Jie; Steffensen, Scott (Brigham Young University)
Faculty Advisor: Steffensen, Scott (Family, Home, and Social Sciences; Psychology); Yorgason, Jordan (Life Sciences, Physiology & Developmental Biology); Sudweeks, Sterling (Life Sciences, Physiology & Developmental Biology)

The prevailing view is that enhancement of dopamine (DA) transmission in the mesolimbic underlies the rewarding properties of ethanol (EtOH) and nicotine (NIC). Although the dogma is that EtOH enhancement of DA neural activity contributes to enhancement of DA transmission, DA neurons are not sensitive to rewarding levels of EtOH. However, VTA GABA neurons are sensitive to low-dose EtOH. We have shown previously that EtOH modulation of DA release in the NAc is mediated by α6-containing nicotinic receptors (α6*-nAChRs), that α6*-nAChRs mediate low-dose EtOH effects on VTA GABA neurons and EtOH preference, and α6*-nAChRs may be a molecular target for low-dose EtOH. The aim of this study was to evaluate EtOH effects on VTA GABAergic input to CINs and DA release in the NAc. Using DIO channel rhodopsin-2 (ChR2) viral injections into the VTA of VGAT Cre mice, we found that VTA GABA neurons send an inhibitory projection to CINs, replicating what has been demonstrated by others. Low-dose EtOH (IC50 = 10 mM) decreased optically-evoked IPSCs (oIPSCs) on CINs and enhanced (EC50 = 10 mM) CIN-mediated spontaneous DA release. Surprisingly, oIPSCs on CINs were not blocked by typical GABAA receptor (GABAAR) antagonists, but by GABAR rho-1 antagonists, suggesting involvement of atypical GABARs on CINs that are postsynaptic to VTA GABAergic input. The α6-conotoxin MII blocked the effects of EtOH on spontaneous DA release and optically-evoked DA release in choline acetyltransferase (ChAT) ChR2 mice. Chronic administration of NIC enhanced EtOH consumption in the drink-in-the-dark procedure and EtOH preference in the CPP procedure and concomitantly enhanced expression of α6*-nAChRs in VTA GABA neurons, without affecting other nAChR subunits. Taken together, these findings suggest that VTA GABA neuron inhibitory input to CINs is modulated by α6*-nAChRs and sensitive to low-dose EtOH, which may underlie the rewarding properties of EtOH.

Allen, Sierra; Meyer-Ficca, Mirella; Wandersee, Miles (Utah State University)
Faculty Advisor: Meyer-Ficca, Mirella (College of Agriculture and Applied Sciences; Animal, Dairy, and Veterinary Sciences Department)

Niacin, a component of vitamin B3, is necessary for the synthesis of nicotinamide adenine dinucleotide (NAD+). NAD+ is an essential coenzyme in several metabolic processes, including those that regulate glucose and fat homeostasis in the body. Niacin is available to humans in its dietary form through foods such as meat, various legumes, nuts and vegetables. Niacin deficiency has been linked to health problems including age-related decline of cognitive abilities, as seen in Alzheimer's disease, and impaired dermal repair. In cases of extreme niacin deficiency, individuals may even develop pellagra, a disease characterized by dermatitis, diarrhea and dementia. Recent studies in niacin deficient mice indicate that niacin deficiency impairs proper glucose metabolism. Compared to mice with adequate niacin levels, niacin deficient mice experience a significant loss of total body weight and body fat and have impaired glucose regulation in response to insulin. We hypothesize that these metabolic defects result from inadequate levels of NAD+ preventing the conversion of lactate to pyruvate in the gluconeogenesis pathway, which in turn results in decreased blood glucose levels. Another potential explanation is that niacin deficiency results in decreased glycogen stores in the liver, also impeding glucose production. To test these hypotheses, we are studying the relationship between dietary niacin and glucose metabolism in a transgenic mouse model with acquired niacin dependency that accurately represents human niacin metabolism. Results of this study will contribute to better understanding of niacin's role in proper glucose metabolism, with important implications for diabetes and other glucose-related diseases, particularly in populations with limited access to quality food.

Garg, Varun (University of Utah)
Faculty Advisor: Balagurunathan, Kuberan (University of Utah, College of Pharmacy; Medicinal Chemistry)

Heparin is a widely-used blood anticoagulant medicine that is used in surgeries and in treatment of circulatory system illnesses. Heparin is currently harvested from live pigs before they are slaughtered. There are many problems with the current production process of heparin. On a chemical level, the drug can be easily and accidentally altered due to its complex chemical nature. In addition, the environmental impact of the pigs that need to be raised on farms and fed is immense. These reasons and more are why bioengineering approaches towards the artificial synthesis of Heparin need to be researched. One of the major obstacles in the synthesis of heparin is the production of Low Molecular Weight (LMW) Heparosan, a chemical precursor. The research goal is to find methods to increase LMW Heparosan yields to a point where those same yields can be used to conduct further exploration into Heparosan oligosaccharide precursors. These oligosaccharides are useful in further understanding heparin structure-function relationships and will eventually help in the goal to find a commercially viable, cost and time effective method of heparin production. Using shake flask bacterial cultures, LMW Heparosan was produced while adjusting variables such as bacterial density at induction and incubation time to maximize yield. Once a sufficient yield was obtained, oligosaccharide structures spliced from the LMW Heparosan were classified for further research. The initial shake flask experiments showed increased yield and purity of Heparosan for each successive trial. Further work is required to scale up production of LMW Heparosan to a mass-market synthetic Heparin but the research conducted shows a promising method to synthesize and improve yields to make that possible.

Clarke, Eliza; Williams, Stephanie; Payne, Andrew; Obray, J Daniel; Yorgason, Jordan; Steffensen, Scott (Brigham Young University)
Faculty Advisor: Steffensen, Scott (Family, Home, and Social Sciences; Psychology)

Dopamine (DA) transmission is a key player in the rewarding aspects of ethanol as well as ethanol dependence. The current dogma is that DA transmission is increased during ethanol exposure via the inhibition of ventral tegmental area (VTA) GABA neurons and that excitation of VTA GABA neurons during withdrawal results in decreased DA transmission. Microglia, the major neuroimmune effector in the brain, may be a key mediator in this process by releasing cytokines following activation. It is also thought that BDNF may mediate this effect. We evaluated the effect of ethanol on cytokine concentrations in the VTA and nucleus accumbens (NAc), and found that low dose ethanol (1.0 g/kg) decreased interleukin (IL)-10 levels, but high dose ethanol (4.0 g/kg) increased IL-10 levels. We also used standard cell-attached mode electrophysiological techniques to evaluate the effects of select cytokines and BDNF on VTA neuron firing rate in vitro. We found no change in firing rate in response to IL-6 and BDNF, but an increase in firing rate in VTA DA neurons in response to IL-10. Consistent with the changes in firing rate, optically-evoked IPSCs were also found to be decreased in response to IL-10. Ex vivo voltammetry and in vivo microdialysis were done to determine whether IL-10 can directly result in an increase in DA release. Although ex vivo voltammetry showed no change in DA release, IL-10 increased DA release in vivo. These findings suggest that the rewarding and/or addictive effects of ethanol may be mediated by cytokines, specifically the anti-inflammatory cytokine IL-10.

Edvalson, Logan; Davis, Morgan; Busath, David (Brigham Young University)
Faculty Advisor: Busath, David (Life Sciences, Physiology & Developmental Biology)

A significant research focus in influenza pathogenesis has been directed towards growth factor receptor tyrosine kinases (RTKs) and their respective phosphorylation cascades. Several recent studies have implicated RTK signaling cascades, that are classically associated with cancer, with increased viral titer. A portion of these studies have focused on early segments of the signaling cascade while others' efforts focus in the late segments. Experiments performed in our lab have identified two receptor pathways—PDGF and VEGF—that, when the receptor inhibited, reduces the efficiency of the influenza virus. These data were achieved using compounds, and variants of compounds, already approved for human use in cancer. Although the drug oseltamivir is already approved for influenza treatment, there is concern for the development of viral drug resistance. The introduction of several types of infection blockers similar to the ones identified by our, and others, laboratories can mitigate viral resistance; like the introduction of several types of antibiotics has reduced bacterial resistance. We hypothesize that these pathways work in multiple parts of the infection cycle ranging from viral endocytosis to the budding off of new virions. Experiments are now under way to determine the specific interactions in these pathways that are important in the viral life cycle.

Wertz, Parker (Weber State University)
Faculty Advisor: Dorsey, Bryan (Weber State University, Geography)

Prevention and predicting spread is the best method of control against invasive species. Land managers require accurate and reliable methods for containment and eradication to prevent land cover change and loss of biodiversity. Ecological niche models exist and are used by ecologists to map habitat suitability, but many rely on presence-absence samples which are difficult to obtain. Maximum entropy species distribution modeling (Maxent) is a popular model that has been increasingly used since it can make valid predictions using presence-only data. Many studies have used Maxent to model species distributions, but few have done so with crowdsourced data since it is more likely to be bias and unreliable. The purpose of this study is to test the robustness of Maxent using crowdsourced presence-only data on Lyrthum salicaria, a perennial herb that invades wetlands and pushes out native flora. The study is set in northern and central Utah, and uses environmental variables in climate, landcover, and topography, with landcover being the most contributive factor to the model. Model performance was very good, even with species data being bias towards areas of higher population, proving Maxent as a worthy method to use in species distribution modeling with crowdsourced species presence data. This results of this study show promise for use in modeling other invasive plants in the future.

Greenhalgh, Mitchell; Merrill, Alex; Lopez, David; Lefevre, Sam; Williams, Greg (Brigham Young University)
Faculty Advisor: Abbott, Ben (Brigham Young University, Plant and Wildlife Sciences)

The presence of sand and gravel pits around Utah are usually accompanied by public complaints of increased negative health outcomes. The primary risk from these areas is crystalline silica (CS)—the molecule released into the air as a result of crushing rocks and sand. Literature has given mixed results of the potentially harmful effects of crystalline silica. To address the potential health risk of Utah residents from living near sand pits, we performed a meta-analysis on CS-related literature to estimate the true effects of CS on human health. We then used GIS software to estimate the total population in Utah that lives within a 5000-meter buffer of the sand pits in Utah. Using Utah cancer data, birth data, and hospital emergency department data, we created risk ratios for residents within the buffer. The meta-analysis concluded that CS is a weak lung carcinogen. Other research suggests that air pollution leads to low birth weight and preterm births. In our study, lung cancer rates were significantly lower in populations within the 5000-meter buffer. We found no evidence of significant adverse birth outcomes as a result of living in close proximity to sand and gravel pits. Non-malignant respiratory disease also had significantly lower rates within the buffer. These findings are important in determining the role of sand/gravel pit operations in disease incidence in surrounding communities. More research is needed to evaluate confounding factors such as smoking prevalence and socioeconomic status and to investigate crystalline silica in non-occupational settings.

Johnson, Erik; Johnson, Ellie; Johnson; Jerald (Brigham Young University)
Faculty Advisor: Johnson, Jerald (Brigham Young University, Biology)

It seems counterintuitive that organisms should evolve handedness (or what we might more broadly refer to as "lateralization"). Individuals who can forage equally well with both hands, who can kick equally well with both feet, who can detect stimuli and orient equally well in both directions, and so on—these individuals should be favored relative to those who are either right handed or left handed. Yet in humans, and in several other species, handedness is common, but we still no very little about why. Here we explore this question using a tropical freshwater fish species with an unusual anatomy. Males have a modified fin—the gonopodium—that they use to internally inseminate females. Interestingly, males are either right or left handed for this structure, which terminates with either a dextral or sinistral twist. In this study, we ask a simple question: is there a link between male gonopodium morphology and male behavioral lateralization. We use a detour test approach to determine how males approach different stimuli, turning either to the left or right to more clearly see each type of stimulus. We focus on how males approach potential mates, predators, and novel items. We predict that males with a dextral gonopodium will orient differently than those with a sinistral gonopodium, consistent with the idea that there is link between behavioral lateralization and morphological handedness. If true, it would suggest that reproductive morphology could be linked to brain and behavioral lateralization in vertebrates.

Backman, Natalia; Ogden, Heath (Utah Valley University)
Faculty Advisor: Ogden, Heath (Utah Valley University, Biology)

Siphlonuridae is a family of mayflies (order Ephemeroptera) that are nicknamed the "Primitive" Minnow Mayfly family. The family traditionally has consisted of four genera; Edmundsius, Parameletus, Siphlonisca, and Siphlonurus, representing twenty six described species. The family Dipteromimidae was described as a sister group to the family Siphlonuridae (Tojo & Matsukawa, 2003), however, past molecular evidence suggests that Dipteromimidae might nest within Siphlonuridae(T. H. Ogden et al., 2009; T. Heath Ogden & Whiting, 2005). This study aims to use more taxa and more molecular data in order to generate more robust phylogeny for these mayflies. Specifically our goals are to (i) test the monophyly of the family Siphlonuridae; and (ii) compare traditional Sanger sequencing loci to newly generated phylogenomic data from a targeted capture sequencing approach.

Faulkner, Megan; Stoneham, Lisa; Brasso, Rebecka (Weber State University)
Faculty Advisor: Brasso, Rebecka (College of Science, Zoology)

Mercury is a toxic heavy metal that poses significant health risks to humans and wildlife. The organic form of mercury, methylmercury (MeHg), is converted from its inorganic form via microbial methylation primarily in aquatic systems. Methylmercury is dangerous because it attaches to proteins in blood and muscle and biomagnifies in food webs. The goal of this project, is to determine mercury concentrations in western spotted orb weaver spiders (Neoscona oaxacensis) collected from two sites on Antelope Island State Park. Previous studies have shown orb weavers associated with the Great Salt Lake ecosystem to accumulate significant methylmercury, connecting the aquatic and terrestrial ecosystems through a shared food web. The Great Salt Lake surrounding Antelope Island has historically shown some of the highest levels of Hg in surface waters in the United States and has both an abundance of orb weaver spiders and their preferred prey—brine flies. We tested the hypothesis that mercury concentrations in orb weaver spiders would differ between two sites on the island based on differences in environmental conditions (salinity) in the water where brine flies develop. All spiders were sexed, weighed, and individually analyzed for total mercury concentration using a Nippon MA-3000 Direct Mercury Analyzer.

Wilson, Nancy; Johnson, Steven (Brigham Young University)
Faculty Advisor: Johnson, Steven (Life Sciences, Microbiology & Molecular Biology)

Diabetes is the seventh leading cause of death in the United States today. Between sixty and ninety percent of diabetics also have sleep apnea. Although both sleep apnea and diabetes engender weight gain, the comorbidity of the two conditions is higher than can be explained by obesity alone.
In this study we explore the advantages of and evidence for the coevolution of diabetes and sleep apnea.
There is a metabolic shift that takes place when the cells of the heart need repair. Normally, hypoxic events cause a shift in heart-cell metabolism toward a high-glucose energy use. This shift mechanism is still fully functional in a diabetic heart cell, but because the underlying diabetes shifts the cellular metabolism to a primarily fatty-acid-based energy use, even a normally functioning hypoxia-induced cascade does not lead to full glucose metabolism or normal cellular repair.
So sleep apnea might serve a useful function in instigating heart tissue repair in cells. This suggests that sleep apnea and diabetes are not just frequently found together, but one condition may be causing the other.
After discussing some of the possible evolutionary drivers for co-adaptation of sleep apnea and diabetes, we examine some of the epigenetic marks associated with the two conditions, laying the groundwork for a better understanding of the underlying etiology.

Caten, Reilly; Valentine, Julie; Miles, Leslie (Brigham Young University)
Faculty Advisor: Valentine, Julie (Brigham Young University, Nursing); Leslie, Miles (Brigham Young University, Nursing)

In the United States, there is a push to mandate submission and testing of all sexual assault kits. A typically-overlooked benefit of testing sexual assault kits is the exoneration of wrongfully accused suspects. Sexual assault kits include DNA samples from the victim of sexual assault, and occasionally are submitted with DNA samples collected from the identified suspect for comparison. Inclusion of suspect samples is beneficial because it can lead to a DNA match with samples collected from the victim or exclude the suspect as the source of DNA. For a sexual assault kit to meet the criteria for "excluded the suspect" a DNA profile had to be developed from analysis of the sexual assault kit evidence which excluded the named suspect with submitted DNA sample.

A large retrospective study of 2,727 sexual assault kits with completed DNA analysis found 66 cases in which DNA findings excluded the suspect. Findings will be presented on descriptive data on cases in which the named suspect was excluded: relationship between victim and suspect, victim loss of consciousness/awareness at time of assault, alcohol/drug use during the assault, suspected drug-facilitated sexual assault, and multiple perpetrator sexual assault. Each of these cases excluding a suspect represents an individual who was erroneously identified, but DNA analysis findings excluded the identified suspect.

Testing sexual assault kits uses science to aid in the establishment of justice. Sexual assault kit testing transcends accusations and labeling to provide factual evidence supporting the claims of the innocent, whether they are identified as victim or suspect. Additionally, the liberation of an unjustly accused suspect promotes the correct identification and conviction of the responsible perpetrator. Thus, mandated testing of sexual assault kit promotes justice for victims of sexual assault and innocent suspects alike.

Smith, Hayden; Leavitt, Steve (Brigham Young University)
Faculty Advisor: Leavitt, Steve (College of Life Sciences, Biology)

Brigham Young University's Lichen Air Quality Biomonitoring Program (Herbarium of Non-Vascular Cryptogams) represents one of the largest and longest-running lichen biomonitoring programs worldwide, with nearly 500 permanent reference plots distributed across the Intermountain West. At each reference site, sensitive indicator lichens are selected for elemental analyses of 25 potential pollutants, with the aim of subsequent resampling every five to 15 years for ongoing evaluations of ecological health. Using elemental analysis (EA) data from the past 30 years, a model for large-scale monitoring has been developed with the aim to (i) improve interpretation of air quality using lichen bioindicators, (ii) establish a framework to integrate future EA samples for comparison to historical data, and (iii) develop a platform in the future to more effectively share these data with land management agencies, research groups, and the broader public. The model will be field-tested with new EA samples collected along the Wasatch Front.

L'Ecuyer, Katia (Utah Valley University)
Faculty Advisor: Gazdik Stofer, Michaela (Utah Valley University, Microbiology)

Streptococcus agalactiae most commonly known as Group B streptococcus (GBS), are encapsulated gram-positive bacteria encountered in approximately 15-40% of pregnant women's urogenital and gastrointestinal tracts. While most women are asymptomatic, GBS colonization of newborns as they pass through the birth canal can lead to sepsis. GBS bloodstream infections are the leading cause of mortality and morbidity amongst infants in the United States. In recent years, several studies have examined the benefits of oral probiotics to promote a healthy vaginal flora and assessed the inhibitory activity of lactobacilli against urogenital pathogens, with mixed results. The purpose of our research is to examine the effect of Lactobacilli on the growth of different GBS serotypes in the vaginal environment using in vitro culture competition experiments. Previously published microbiome studies were used to determine the dominant species found in the vaginal microbiota. We are examining the growth rate of GBS when co-cultured with vaginal microflora species, both individually and as a mixed community. This will provide a baseline regarding what strains of GBS could easily colonize the vagina in high levels when in competition with different normal flora communities. Different species of probiotic Lactobacilli will then be added to the vaginal culture collection to examine if there is an effect on GBS growth. Our goal is to identify probiotic species that prevent or slow the growth of GBS in a vaginal community.

Small, Christina; Obray, J Daniel; Steffensen, Scott (Brigham Young University)
Faculty Advisor: Steffensen, Scott (Family, Home, and Social Sciences; Psychology)

Recent studies have shown that administration of dopamine in the periphery (outside of the brain) produces a robust enhancement of dopamine release in the nucleus accumbens and alleviates cognitive deficits associated with schizophrenia-like and attention deficit hyperactivity disorder (ADHD)-like phenotypes in rodent models. Despite this, the mechanism whereby peripheral administration of dopamine produces these effects is unknown as dopamine does not cross the blood brain barrier. Activation of dopamine 2 receptors on circulating leukocytes encourages extravasation and can trigger production and release of cytokines such as TNF-_ and IL-10 as well as IL-1_. IL-1_ and IL-10 are both known to enhance dopamine release. In this study we demonstrate that the effects of intravenous dopamine on dopamine release in the NAc are mediated by peripheral dopamine 2 receptors. Additionally, we show that intravenous dopamine is rewarding and that these rewarding effects can be blocked by antagonism of peripheral dopamine 2 receptors. As many drugs of abuse enhance plasma dopamine levels this research elucidates a secondary pathway which may play a role in the development of substance use disorders.