2020 Abstracts

Thornton, Sherie; Cardona,Rebecca (Weber State University)
Faculty Advisor: Culumber, Michele (Weber State University, Microbiology); Oberg, Craig (Weber State University, Microbiology)

Lactobacillus wasatchensis was initially isolated from cheese produced at Utah State University and was found to be a Non-Starter Lactic Acid Bacteria (NSLAB) that causes late-gas production in cheese that can damage packaging and produce defects in the cheese. The goal of this project was to locate an environmental reservoir for Lactobacillus wasatchensis. Five samples of silage that were in different stages of fermentation and content and raw milk samples were obtained at the Utah State University dairy. Samples were serially diluted, plated on de Man, Rogosa and Sharpe agar supplemented with 1% D-Ribose (NRS-R) and incubated anaerobically for 5 days. Colonies that looked like potential Lb. wasatchensis were selected and regrown for isolation. All isolates were gram-positive rods. The isolates were further grown in broth for DNA extraction, sequencing, and analysis with API 50 carbohydrate panel (API 50CH). The API 50CH results were significantly different from Lb. wasatchensis, which only demonstrates use of ribose in this assay. Sequencing of the 16S rRNA gene, however, produced a match to three isolates from two different silage samples that had 99% sequence identity to Lb. wasatchensis. Further analysis of the isolates is being done to confirm this finding and describe the organism isolated from the soil. We hypothesize that these organisms are very closely related to Lb. wasatchensis and that silage could be an environmental source of contamination.

Verstegan, Richard; Foxe, John; Arblaster, Paul (Utah State University)
Faculty Advisor: Sand Alexa (Caine College of the Arts, General Studies (Arts)); Duncan, Jennifer

Horrifying and under scrutinized the Theatrum Crudelitatum Haereticorum Nostri Temporis (1587) depicts endless pages of sixteenth century torture methods inflicted upon Catholic martyrs, leaving sufficient room for questions waiting to be answered. The largest and most pressing question this book offers is why it was created. When exploring different conclusions to such a question it is important to understand the contextual history and nature of a rare object such as this. There is probable cause that this book was created in response to John Foxe's Book of Martyrs, which was published 24 years prior to the publication of the Theatrum Crudelitatum Haereticorum Nostri Temporis. It is also possible that the author of the Theatrum Crudelitatum, Richard Verstegan who was an Anglo-Dutch Catholic, was a propagandist supporting the idea that martyrdom was a form of resistance to oppression of religion at this point in history. There is sufficient evidence supporting this theory. In Foxe's Book of Martyrs there are limited illustrations of torture merthods where as in Verstegan's Theatrum Crudelitatum there is an overwhelming amount of vivid and equally horrifying engravings depitcing Catholics as weak. It is equally important to take a look into the author's personal history to gain more explanations for the creation of the Theatrum Crudelitatum Haereticorum Nostri Temporis. Richard Verstegan was an Anglo-Dutch Catholic that studied English at Oxford University without obtaining a degree. It is thought that he left due to his religious beliefs at the time. He was later a propagandist of Duke and Guise before settling in Antwerp, Belgium where he published and illustrated his work. This rare book even served as a precedent as to what happened to those not of the Protestant faith. The Theatrum Crudelitatum Haereticorum Nostri Temporis by Richard Verstegan was created for both personal reasons and propagandistic opportunity.

Call, Emily; Manesse, Alana (Utah State University)
Faculty Advisor: Colton, Jared (College of Humanities and Social Sciences, English Department)

What visual rhetoric was used in the women's suffrage movement and how was it effective? There were many forms of persuasion in the women's suffrage movement, some of the greatest examples being the visual rhetoric. Rhetoric in this analysis will be defined as linguistic and visual elements crafted with the purpose to persuade the viewer to believe in the presented truth and to conform with the represented female identity. For this presentation we will focus on two political cartoons from the suffrage movement: one pro-suffrage piece, Henry Mayer's "The Awakening" (1915), and one anti-suffrage piece, "Looking Backward" by Laura E. Foster (1912). While suffrage is often discussed through a feminist lense, we will apply a cultural rhetoric analysis as our primary research method with supporting analysis coming from a feminist perspective. Our analysis will use cultural frameworks with a focus on the cultural theorist Stuart Hall. Cultural rhetoric focuses our analysis on the values and practices of English and American culture in the 20th century rather than solely looking at the object of analysis. In that context, we will examine the rhetorical strategies the artists use to craft arguments to persuade the viewer to accept the "truth" they are presenting. Through the comparison of opposing pieces of propaganda, we will also show that truth is relative to the viewer. Through Hall's frameworks, we will analyze ideas about fixed meaning in the images, female representation in a culture, and the audience's response to rhetoric. As we examine these particular elements in conjunction with cultural rhetoric, the audience will gain insight into how pro-suffrage and anti-suffrage propaganda used similar rhetorical techniques to persuade its viewers.

Andersen, Jacob (Brigham Young University)
Faculty Advisor: DeSchweinitz, Rebecca (Brigham Young University, History)

During the 1912 Lawrence, Massachusetts Textile Strike, Elizabeth Gurley Flynn, a speaker and organizer for the Industrial Workers of the World (IWW), planned to address local workers at a Greek Catholic Church. Before she stepped to the pulpit, a local priest forbid her from speaking because she was a woman. She only spoke to the audience that night after she convinced the priest that she "spoke as an organizer, not as a woman." This incident hints at the complex and often contradictory role of gender in the IWW. Indeed, many scholars have argued that while the IWW preached universal human equality, in practice they maintained a limited, domestic vision for women. Francis Shor, in particular, has argues that the IWW's approach to gender constituted a kind of "virile syndicalism," in which their aggressive tactics were a form of "masculine posturing" in defiance of industrial capitalism. While an important insight about the role of gender in the IWW, few scholars have used his theory to understand women's experiences in the IWW. This paper will examine the role of gender in the IWW through the lives and experiences of three women: Elizabeth Gurley Flynn, Matilda Robbins, and Jane Street. All three women came from different backgrounds and made unique contributions to the IWW, yet the IWW's virile syndicalism circumscribed their activities within the bounds of the IWW's narrow gender ideology. The lives of these women suggest that virile syndicalism in the IWW limited the role of Wobbly women and curbed the IWW's ability to effectively organize workers in the long term. Indeed, the IWW notoriously struggled to maintain an organized base of workers; their distinctly gendered tactics may help explain why.

Lengele, Alexius (Weber State University)
Faculty Advisor: Seaboch, Melissa (Salt Lake Community College, Anthropology)

Primate defecation behaviors contribute notably to forest growth and diversity because primates are important seed dispersers in their environment. Understanding these defecation patterns is important to understand how primate populations impact tropical dry forests. Preexisting research on Allouatta seniculous (red howler monkeys) avoided defecating near their foraging and resting areas, potentially to avoid parasitic infection from contaminated feces. The goal of my research is to test whether the same pattern is found in A. palliata, the mantled howler monkey. I predicted that A. palliata would not defecate near their food resources as a parasite-avoidance behavior. La Selva Biological Station, where this research was conducted, is located in Sarapiqui, in northeastern Costa Rica. Data were collected in May 2017 at the beginning of the rainy season. Using all-occurrence sampling, I recorded all defecation events for A. palliata and whether any group members were feeding. I recorded 15 instances of defecation. Howler monkeys defecated in the same area where they had been feeding 46.7% of the time (n=7) and they defecated in an area where feeding did not occur 53.3% of the time (n=8). My hypothesis, that A. palliata would not defecate near food resources, was not supported as the data showed no bias toward non-feeding areas versus feeding areas. These results differ from prior research of Alouatta in the Amazon Basin, Venezuela, and Brazil, which all reported evidence of parasite-avoidance in defecation behavior. My sample size is small, and my data were collected in a short time span, likely contributing to this discrepancy. Additionally, the parasites infecting those species in South America may not be present in this Central American location, suggesting a lack of the need for this adaptive behavior.

Figgins, Anna C.; Hunter, F. Kate; Kapheim, Karen M. (Utah State University)
Faculty Advisor: Kapheim, Karen (College of Science, Biology Department)

Certain species of ants, bees, and wasps have some of the most sophisticated forms of cooperative behavior known throughout the animal kingdom. These eusocial insects live in large family groups made up of castes (e.g., queens and workers) that specialize on different tasks within a colony. In many species, division of labor between queens and workers is associated with behavioral and physiological traits such as dominance interactions, ovary maturation, and lipid stores. Remarkably, these large phenotypic differences between castes emerge from a shared genome. This suggests caste differences stem from changes in how shared genes are regulated. We have been investigating the role of a small regulatory molecule (microRNA miR-13b) as a potential regulator of division of labor in bumble bees (Bombus impatiens). We tested the hypothesis that miR-13b regulates division of labor by inhibiting its function using small-interfering RNA (siRNA). We inhibited miR-13b function by injecting a synthetic antagonist of miR-13b (antagomir) into the abdomens of live bees. The average expression of miR-13b in the fat body of bees that received the antagomir injection was 0.52 relative to those that received the control injection (n = 7). This inhibition of miR-13b expression was accompanied by a significant decrease in fat body size. However, the influence of the antagomir only lasted 1 day. Future experiments will determine if the antagomir can influence gene expression longer than 1 day and assess physiological and behavioral changes in B. impatiens after miR-13b is inhibited. Knowledge gained from this study allows us to understand more about the mechanisms underlying social behavior in bees and helps us investigate how behavior is regulated by gene expression.

Stapleton, Michael; DeRose, Justin. (Utah State University)
Faculty Advisor: DeRose, Justin (S.J. & Jessie E. Quinney College of Natural Resources, Wildland Resources Department)

Ponderosa pine (Pinus ponderosa) is the most widespread coniferous tree in North America, occurring from Mexico to British Columbia and from California to Nebraska. Surprisingly, however, P. ponderosa is largely absent within the center of this range. Previous studies suggest that this absence may be linked to a range of climatic variables, but collectively fail to identify specific climate-growth responses. Using dendroclimatology, we will analyze how a disjunct population of P. ponderosa in northern Utah responds to local climate conditions. We seek to identify which of these variables the species is most sensitive to by correlating the population's average annual growth to a variety of climate composites. Similar tests will be conducted across three treatment blocks throughout the stand in order to distinguish if previous management altered the population's resilience to climate. Our results will help explain the current distribution of P. ponderosa and suggest how that distribution may respond to changing climate conditions.

Lahy, Neve (Westminster College)
Faculty Advisor: Kruback, Matt (Westminster College, Art)

Tree of Trees
Neve Lahy:
The Uinta-Wasatch-Cache National Forest is a group of national forests within our Wasatch Mountains. The forest is a host to many species of trees, both coniferous and flowering. I have chosen seven of each of the most prominent local cone bearing and flowering trees to focus on.
The trees are a crucial part of the ecosystem and participate in many symbiotic relationships. Without the trees the ecosystem wouldn't survive. It is important to acknowledge the vitality of our local forests not only for us, but for all other organisms dependent on the trees.
A phylogenetic tree is a branching diagram showing the evolutionary relationships among various biological species. Phylogeny is based upon similarities and differences in their physical or genetic characteristics. The branches indicate closeness in relation to one another. The species nearest to each other on the phylogeny are the most closely related organisms based on special derived traits.
Learning how to identify the trees that I am so often surrounded by has brought me a deeper appreciation and love for the organisms around me. Now that I can spend time in trees and know exactly what I am looking at has given me a broader understanding of how the trees not only interact with me but the other ecological factors present in the area. I feel more keenly aware of what it takes to protect these trees and enjoy them more respectfully.

Funk, Stephen; Wood, Jacob; Catmull, Ashley; Martin, Brett (Brigham Young University)
Faculty Advisor: McCleary, Bill (Brigham Young University College of Life Sciences, Microbiology & Molecular Biology)

The survival of the model microorganism E. coli depends largely on its ability to regulate the concentration of nutrients in its cell. This regulation often relies on complex systems of both cooperative and competitive enzymes. One of these enzymes, encoded by the YjbB gene, was known to play some role in phosphate regulation. However, its exact function had not yet been characterized. We attempted to deduce the function of the YjbB-encoded protein in the context of two other phosphate regulatory systems: the PitA/B phosphate transport system and the PpK/X polyphosphate storage system. Using comparative growth curves in both phosphate-rich and phosphate-deprived media, we found that the YjbB-encoded protein protects against phosphate poisoning in the absence of phosphate exporters, suggesting that the protein in question functions primarily as a phosphate exporter as well.

Buehler, Nate; Hoehn, Nick; Stokes, Britt; Tyler, Areiann; Dr. Kopp, Olga (Utah Valley University)
Faculty Advisor: Kopp, Dr.Olga (Utah Valley University, Biology)

Bacterial infections are difficult to treat with antibiotics because of the protective nature of the biofilms produced by bacteria. Biofilms are a common cause of nosocomial and medical devices-related infections. The current treatments for biofilms include mechanically removing the biofilm itself or by treatments with antibiotics. Biofilms usually become resistant to drugs because of the higher frequency of mutation and horizontal gene transfer compared to planktonic cells. Liposomes are promising delivery systems because of their small size, surface characteristics and ability to encapsulate drugs and other molecules. Liposomal particles can slowly release the encapsulated drugs, increasing their distribution in targeted areas. Studies have shown that the fusion between liposomes and bacterial cells enhances the penetration of antibiotics. The purpose of this study is to form liposomes to encapsulate Gentamicin and characterize the formation and characteristics of these liposomes. Liposomes will be formed using the thin film hydration method and characterized using a scanning electron microscope. This project will present an analysis of the use of different ratios of phospholipids and cholesterol to evaluate the stability and ability to carry Gentamicin.

Call, Tanner; Bean, Joseph; Chaston, John (Brigham Young University)
Faculty Advisor: Chaston, John (Life Sciences, Plant and Wildlife Sciences)

The gut microbiome, or the microorganisms that colonize the GI tract of all macro-organisms, plays a significant role in host health and physiology. In a study last year, I found that the microbiome of D. melanogaster has a direct influence on dietary preference using a well-established, automated feeding assay. In this study, I extend these findings by performing a metagenome-wide association (MGWA) screen to predict bacterial genes responsible for the effect. Specifically, I measured dietary preferences in flies mono-associated with each of 40 different bacterial species. My mentor compared the dietary preference of these flies with the genomes of their associated bacteria using a MGWA. This analysis predicted 1932 bacterial genes that could be responsible for the feeding preference phenotype. I selected the top 22 genes, including all uncharacterized genes, for which we have knock-out mutants in a laboratory stock of bacterial mutants. I will test if these genes are necessary for inducing specific host feeding preferences by comparing feeding preferences of flies mono-associated with a bacterial mutant with controls, using a generalized mixed linear model. These results will help us understand how different members of the microbiota can influence animal feeding behaviors.

Perriton, Kiersten; Eppley, Sydnie; Young, Colleen (Utah Valley University)
Faculty Advisor: Jensen, Francine (Utah Valley University, Nursing)

Purpose: To determine the preparedness of new graduate nurses to practice after completion of nurse residency programs.

Background: New graduate nurses often feel anxious, concerned or ill prepared to practice as nurses on their own. New graduate nurses worry if the residency program has prepared them for their first nursing job sufficiently for them to gain enough confidence and skill to be on their own. Studies on nurse residency programs show that nurses who complete one-year nurse residency programs had remarkable increases in communicating with family, patients and team members, confidence in skills, and ability to prioritize and organize work. There is a need for continued education, such as nurse residency programs and an active interest in new graduate nurses, such as involved mentors or preceptors to help strengthen clinical reasoning and preparedness of new graduate nurses. Stress in new graduate nurses can impede patient safety and can result in the new nurse quitting the position. When new graduates have designated mentors, fit well with unit socialization, and are oriented to multiple areas, it has been identified that it can build their confidence levels and improve retention rates of new graduate nurses. Nurse residency programs need to provide high quality orientation to help them master their careers.

Significance: This research can give insight into whether the implementation of nurse residency programs helps new nurses feel ready to practice on their own. This information may help provide increased safety and quality of care to patients due to new graduate nurses feeling competent and confident to carry out the patient's health care plan. This can help healthcare companies determine if current programs are effective and what needs to be changed. New nurse graduates may gain confidence, practice with increased patient safety, and corporations may retain the new nurses they have just trained.

Boatright, Greggory; Medrano, Braxton; Goeckeritz, Joel (Brigham Young University)
Faculty Advisor: Roeder, Beverly (Brigham Young University, Life Sciences)

Schwann cells play a major role in helping heal injured nerves. They help clear debris, produce neurotrophins, upregulate neurotrophin receptors, and form bands of Büngner to guide the healing nerve. But nerves do not always produce enough neurotrophins and neurotrophin receptors to repair themselves. Nerve growth factor (NGF) is an important neurotrophin for promoting nerve healing and lysophosphatidylcholine (LPC) has been shown to stimulate NGF receptors (NGFR). This study tested the administration of a single intraneural injection of LPC (1 mg/mL for single LPC injection and 10 mg/mL for multiple LPC injections) at day 0 and one (day 7), two (days 5 and 7), or three (days 5, 7, and 9) injections of NGF (160 ng/mL for single injections and 80 ng/mL for multiple injections) to determine baseline effects on crushed sciatic nerves in rats. The rats were randomly divided into four groups: control, crush, crush-NGF, and crush-LPC-NGF. The healing of the nerves was measured weekly by monitoring gait; electrophysiological parameters: compound muscle action potential (CMAP) amplitudes; and morphological parameters: total fascicle areas, myelinated fiber counts, fiber densities, fiber packing, and mean g-ratio values at weeks 3 and 6. The crush, crush-NGF, and crush-LPC-NGF groups statistically differed from the control group for all six weeks for the electrophysiological parameters but only differed from the control group at week 3 for the morphological parameters. The crush, crush-NGF, and crush LPC-NGF groups did not differ from each other over the course of the study. Single injections of LPC and NGF one week apart or multiple treatments of NGF at 5, 7 and 9 days post-injury did not alter the healing rate of the sciatic nerves during weeks 1-6 of the study. These findings are important to define the baseline effects of NGF and LPC injections, as part of a larger effort to determine the minimal dose regimen of NGF to regenerate peripheral nerves.

Hyman, Rachel; Light, Tim; Montague, Angela; Lucero, Jessica (Utah State University)
Faculty Advisor: Lucero, Jessica (College of Humanities and Social Sciences; Sociology, Social Work and Anthropology Department)

Refugees are recognized as having unique and complex health needs which demand attention upon arrival and throughout the resettlement process (Agrawal & Venkatesh, 2016). Refugee populations are particularly vulnerable to health disparities due to push factors influencing forced migration (Tempany, 2009). These factors include exposure to political violence, natural disasters, and other forms of anthropogenic conflict (UNHCR, 2014). Refugees may arrive in the host country with undiagnosed and untreated health issues, such as infectious diseases, mental distress due to prior traumas, undiagnosed chronic illness, and nutritional deficiencies (Deen & Bridges, 2011). Refugee populations represent high rates of mental and physical health problems compared with the host population (Kirmayer, Gomez-Carrillo, & Veissi�re, 2017). Additional dynamics compound health disparities facing refugees. These include: language barriers, unfamiliarity with a complex health care system, limited health literacy, and inadequate alignment of medical treatments with religious and cultural beliefs (Bowen, 2001; Brown, Carroll, Fogarty, & Holt, 2010). Though there is robust scholarship in the literature and analysis of prevalent health disparities and needs of refugees post-resettlement, few studies address the specific health care concerns of refugees resettled in northern Utah. This study seeks to expand previous findings related to the most salient health care issues facing the refugee community and develop community response to address health disparity. A secondary aim of the research is to determine best practices for the provision of appropriate and effective health services.

Miles, Leslie; Valentine, Julie (Brigham Young University)
Faculty Advisor: Miles, Leslie (Brigham Young University, College of Nursing); Valentine, Julie (Brigham Young University, College of Nursing)

1 in 10 adolescents (13-18 years old) reported sexual violence in the dating environment with a prevalence rate of 14% among girls and 8% among boys (Wincentak et al., 2017). Additionally, Arliksson-Schmidt et al. (2010) found that adolescent girls with a physical disability or long-term health problem were more likely to experience sexual violence (p=.003). The current literature is limited on describing the characteristics and vulnerabilities of adolescent sexual assault patients. Researchers have found multiple negative sequelae in adolescents who have been sexually assaulted, which includes the following; major depressive disorder, suicide risk, agoraphobia, alcohol abuse & dependence, and substance abuse & dependence (Walt et al., 2014).

To better understand adolescent patients and to guide nursing care, a large (n=5,600) retrospective chart review of SAFEs documentation (2010-2018) examines adolescent descriptors (age 14-18) in a Western state in the United States. Variables include gender, race, age, relationship to suspect, online meeting, pre-existing medical conditions, pre-existing mental illness, previous history of sexual assault, number of non-anogenital injuries, number of anogenital injuries, alcohol and drug use, multiple suspects, and suspect actions (violent physical acts). Comparisons to patients over the age of 18 years will be provided.

The purpose of this podium presentation is to review research findings concerning the demographics and characteristics of adolescents seen for sexual assault forensic examinations to better inform health care professionals. Discussion will include possible medical and nursing interventions to mitigate the potential harmful effects of adolescent sexual assault.

Greaves, Aspen ; Allison, Jim (Brigham Young University)
Faculty Advisor: Allison, Jim (Family, Home, and Social Sciences; Anthropology)

Portable x-ray fluorescence (PXRF) analysis is a quantitative measure of the presence of an element. Elements from painted red ware sherds from the San Juan region were measured on painted and non-painted portions of the sherds, demonstrating the presence of elements in the paint as opposed to the paste. In particular, manganese, lead, and copper, along with a more ubiquitous iron, were found in the paint. The presences of manganese indicates the intention for the paint to be black when fired, a useful tool in identifying sherds. At Site 13 (42sa13) for example, which was burned, the sherds can appear to be Abajo red-on-orange when they may better be described as Bluff black-on-red. Lead and copper are a bit less straight-forward. Lead appears rarely, and may have no purpose, or could be an intentional sourcing choice. Another question comes from the presence of copper, which may have created a turquoise paint pre- firing. Turquoise is an important color in Pueblo color theory, and so if copper does create a turquoise paint, the presence of copper may be deliberate. Variation in paint, particularly crossed with neutron activation data, leads to a firmer understanding of decisions inherent in the ceramic creation process.

Christensen, Kate L.; Hinkson Jr, Kent D.; Brooks Malisa M.; Bryan, Craig J.� (Utah Valley University)
Faculty Advisor: Hinkson Jr, Kent (Utah Valley University and University of Utah, Psychology); Brooks, Malisa (University of Utah, Psychology)

Among the staggering rates of sexual assault, little attention has been directed towards the specific risk factors involved with coercive sexual assault. The few studies that have been conducted focus solely on college campuses. Studies have found that the potential risk factors between forced sexual assault and coercive sexual assault are fundamentally different. While forced sexual assault is associated with environmental risk factors such as alcohol, coercive assault risk factors have a stronger relationship with the victim's level of depression and self-esteem. Though depression and low self-esteem can affect people for a variety of different reasons, age tends to be a reliable predictor of both of these risk factors. Depression and low self-esteem have some of the highest rates among teens and young adults. When focusing on the Military, we see depression rates higher than that of the general public, with similar patterns of teenagers and young adults (18 to 25) being the most affected. With the knowledge that depression and low self-esteem are risk factors in coercive sexual assault and knowing an age group that is more likely to be depressed and struggle with esteem, we should expect to see a relationship between age and being a victim of coercive sexual assault. In a survey consisting of veterans, age had a mild relationship with coercive rape, with younger people being more susceptible and their older counterparts holding less risk. When focusing on other distinctions of sexual crimes such as harassment, assault, and rape, age was not found to be a risk factor. Knowledge of the relationship between coercive sexual assault and age carries important implications, particularly in the form of education and support. Leaders armed with this data can inform young people of this potential risk, provide preventative strategies, and recommend therapy or support groups when necessary.

Oram, Kathryn; Griffiths, Alayna (Utah Valley University)
Faculty Advisor: Gazdik-Stofer, Michaela (Utah Valley University, Biology)

The World Health Organization currently estimates that 4,384 individuals die per day due to complications of Mycobacterium Tuberculosis and affects 1.8 million people worldwide as it infects individuals through air droplets from a cough or sneeze. Cyclic adenosine monophosphate (cAMP) is known to be a major component in TB because it acts as a macrophage inhibitor that is responsible for blocking the immune defense allowing the M. Tuberculosis to rapidly replicated within cells. The function of cAMP in TB patients is known but the focal point of our research is why and how the increased levels of cAMP effects patients that are infected with TB. Our team uses mutated M. Smegmatis bacteria due to the comparable levels of cAMP secretion and high pathogenicity of M. Tuberculosis. We are currently screening the cAMP secretion in 1,000 mutated M. Smegmatis colonies to identify secretion differences from the wild-type M. Smegmatis. The mutants samples with high variation from the wild-type will be sequenced to identify the genes and determine the proteins that are present. Finding the genes and proteins can help understand why and what causes the inflation on cAMP secretion in TB patients.