2014 Abstracts

Teresa Wilson, Utah Valley University Health A critical issue in breast conservation surgery (lumpectomy) for breast cancer treatment is ensuring the tissue surrounding the excised tumor, the margins, are cancer-free. In collaboration with the Huntsman Cancer Institute at the University of Utah, students from Utah Valley University are using high-frequency (HF) ultrasound to test the pathology of lumpectomy surgical margins. This pre-clinical study is a blind study which will involve 80 patients, approximately 320 specimens, and use traditional pathology as the “gold standard” for measuring the HF ultrasound method’s accuracy. Ultrasonic waveforms of margins were acquired at the Huntsman Cancer Hospital in pitch-catch and pulse-echo modes using 50-MHz, 6.35-mm diameter transducers. The data were analyzed to obtain ultrasonic parameters such as wavespeed, attenuation, specimen thickness and spectral peak density (the number of peaks and valleys in a HF ultrasonic spectral band). The objective of this paper is to search for trends in the data acquired to date to provide an assessment of reliability, stability, and robustness of the study.

Ashley Ostler, Southern Utah University Health Research indicates that collegiate athletes may be at risk of developing disordered eating and other distorted behavioral patterns. This may be due, in part, to the aesthetic and performance demands of their sports. The purpose of this study is to discover if female athletes acquire increased negative views towards their body image and food due to the pressures of being a collegiate athlete at Southern Utah University (SUU). An anonymous survey was given to approximately 150 female athletes participating in a variety of sports inquiring about body perception and eating behaviors relative to their sport. The survey evaluates the athlete’s attitude toward food, their relationship with food, if there are outside influences that affect how they eat, and if they are satisfied with their weight. Athletic teams were chosen based on meeting two of the three following requirements: tryouts are held, scholarships are offered, and there are regular competitions or performances. Athletic teams involved in the survey include tennis, volleyball, track & field, cross country, softball, basketball, gymnastics, soccer, drill team, ballroom dance and cheer. The data will provide information regarding Southern Utah University female athlete’s body perceptions and eating behaviors. Results will be compared to a similar study conducted five years ago at SUU.

Taylor Colvin, Utah Valley University Health Introduction: There are millions of people in need of prosthetics worldwide and only a very small percentage currently have access to appropriate prosthetic care. As amputees are unable to afford a prosthetic, they are often incapable of maintaining many social interactions and provide for their basic needs. Utah Valley University’s Mobility Mission traveled to Guatemala to manufacture prosthetic limbs for those individuals in need. Our research can also encourage manufacturers to continue developing a better affordable prosthetic that can be donated to those amputees in need to restore quality of life to those individuals.

Josh Lee, Dixie State University Health According to the World Health Organization (2012), health care systems in Central America rank in the top 50% of the 190 ranked countries in the world, yet thousands of people are in need of simple medical attention and supplies every day in these areas. Countries such as Honduras lack virtually all access to health services, with 83% of the country’s population without health insurance, and 30% with no health care access at all (Pearson et al., 2012). To address this issue, a 2013 Dixie State University study conducted by the author, an Integrated Studies major with disciplinary emphases in Spanish and Biology, interviewed fifty adult Central American immigrants at a Southern Utah health care facility with the aim to providing student groups embarking on health service projects a researched list of vitally needed medical supplies. Participants in the study were asked to provide information regarding their health care experiences in their countries of origin and draw upon their comparative experiences with health care in the United States in order to determine areas of need. The forty individuals in this study represented a total of seven Central American countries and the interviews were conducted in Spanish and translated into English. The interviews were conducted over an 8-week period of time and results suggested that diabetes, cold, flu, antibiotics, and anti diarrhea medicines and supplies are a few of the needed health care items in these neighboring countries. Although statistical significance was not a feature of this study due to the number of participants, significant consistencies in responses by former residents of a variety of Central American countries suggested that some medical needs are regionally shared and that larger scale follow-up studies may reliably identify these as key resources for student medical delegations traveling to Central America.

Stephen Clarkson, Brigham Young University Health While there is an abundance of mobile health apps for weight management on the market today, almost all focus entirely on net caloric intake (exercise minus calories consumed). Recording daily caloric intake can be cumbersome, inefficient and inaccurate. One rising suggestion in the health field for reaching weight goals is objectively recording hand-to-mouth movements (HTMMs) during meals throughout the day. This method focuses entirely on portion control and if any improvements are to be made in this area, an effective method of activity recognition must be developed. In this paper, we report our efforts to classify HTMMs and non-HTMMs in an effort to automate counting the number of HTMMs during meals throughout the day. We also report on the performance of several base-level classifiers such as k-NN, Naive Bayes and Decision Trees as well as meta-level classifiers (Voting, Bagging and Boosting).

McKay Frandsen, Utah Valley University Health INTRODUCTION

Charity Anne Rowley, Brigham Young University Health In 2010, The Office of the Inspector General of the U.S. Department of Health and Human Services reported that adverse events during hospitalization contribute to the deaths of 180,000 patients each year! The purpose of this study is to improve patient safety in the hospital by understanding the role of the charge nurse(the 24/7 nursing supervisor in hospital units).

Jennifer Orton, Brigham Young University Health In contrast to the success of America’s vaccination rates among children, adult vaccination rates remain suboptimal. Some adults, such as school employees, are in a position where they can easily contract and spread vaccine-preventable communicable diseases, yet mandatory vaccinations are usually lacking in this population. Schools may easily become communicable disease outbreak centers, especially for highly contagious diseases such as measles and influenza. The purpose of this study was to evaluate why rural Utah school employees remain vaccinated for measles and influenza, as well as to discover the perceptions of these school employees regarding mandatory vaccination policies. Of the 835 respondents, 71.7% reported they received the measles vaccination as a child, although only 26.9% reported having a measles booster vaccine as an adult. When asked why they had not received the measles booster, 46% of participants stated they were unsure if they needed one. Other participants also reported lack of an adult measles booster vaccine because it was not required by the school district (5%) and that they did not perceive the need because they were healthy (5%). Even more concerning, over half of the school employees (51%) had not received their influenza vaccine for the current year. When questioned about their lack of influenza vaccination, 26.4% believed the vaccine was ineffective and 25.4% were noncompliant because they did not have the time to get the vaccination. When questioned about their perceptions regarding mandatory vaccine policies for school employees, four major themes emerged: 1) adults should retain agency; 2) vaccine effectiveness was not guaranteed; 3) adults do not need vaccinations; and 4) cost of vaccines. Further education is needed regarding the need for adult vaccinations, especially among school employees in rural Utah. To control the spread of communicable diseases, school districts may consider mandatory vaccination of school employees.

Emily Matson, Brigham Young University Health Cancer, despite its common association with old age, is a leading cause of death for children age 1-14, second only to unintentional injuries. Improvements in diagnosis and treatment are increasing childhood cancer survival, and more research is needed on the risk of subsequent cancer following an initial diagnosis, of either the same or another site. Research on associations between childhood and secondary cancers may improve patient follow-up by providing health professionals with information about which subsequent cancers are likely to occur, which screenings to conduct and what symptoms to look for.

Wesley Tietjen, Dixie State University Health High risk sexual behaviors, such as having unprotected sex, are a community health concern because of the heightened susceptibility to sexually transmitted diseases. An estimated 19.7 million people are newly infected with a sexually transmitted disease annually. Young people (ages 15-24) account for nearly half of all new incidences (CDC, 2008). Considering these statistics, the author, a Dixie State University Integrated Studies undergraduate student, conducted an interdisciplinary study to determine the correlation between the degree of self-reported religious devotion of Latter Day Saint (LDS) singles and their likelihood of purchasing or using protection during premarital intercourse. Seventy five students attending Dixie State University in Saint George, Utah participated in this study. Participants completed a 15-item survey consisting of questions inquiring how religious they consider themselves and their feelings towards premarital sex. Of the non-married participants who engage in sex, only 28% (n=4) of LDS respondents reported consistent use of birth control, compared to 84% (n=16) of non-LDS individuals. These results are suggestively significant and are consistent with findings from studies conducted by Douglas Kirby (2007) and others who have demonstrated that teaching abstinence, without providing education about birth control methods other than abstinence, leaves individuals unprepared to practice safe sex if the occasion arises. The relevance of the Dixie State University study to previous more broadly conceived research will be presented along with recommendations for future research.

Andrea Quiroz, Utah Valley University Health The stimulation and inhibition of tissue vascularization has important applications to tissue engineering and oncology. Approaches to quantitatively evaluate neovascularization in vivo in adult animals with differentiated tissue include both invasive methods that use an implanted or injury-induced matrix in the study organism, or noninvasive small animal imaging methods such as MRI, CT, and PET. The objective of this study was to determine if ultrasonic spectra in the 10-100 MHz range could be used as an in vivo neovascularization assay. Numerical simulations and phantoms were used as model systems to test the feasibility of the approach. The simulations modeled ultrasonic scattering from microscopic vascular networks using randomly oriented cylinders to represent blood vessels and cylindrical wave functions to represent ultrasonic waves. Phantoms were fabricated from a gelatin-soluble fiber mixture and agarose gel. The agarose gel was embedded in the gelatin-fiber in order to simulate vascular tissue. Ultrasonic tests were performed using two broadband ultrasonic transducers centered at 50 MHz. Pitch-catch data were collected and analyzed by calculating the peak density of the ultrasonic frequency spectrum, which is the number of peaks and valleys in the spectrum (a measure of the spectrum’s complexity). Control specimens were tested to determine the differences in the sound wave properties between the gelatin-fiber and the agarose gel. The controls demonstrated the instrument’s ability to differentiate between the two types of material. Measurements of agarose inclusions in the gelatin-fiber showed a significantly higher peak density than those for the control gelatin-fiber. These results indicate that the agarose gel structures are scattering sound to a greater extent than the gelatin-fiber alone. The results thus far have provided a strategy for proceeding with the project in the future. The phantoms will be refined and made more realistic to produce an adequate mimic for vascular tissue.

Elise Corbett, Brigham Young University Health Background

Tim Smith, Brigham Young University Health Previous research studies have confirmed that life-threatening illness adversely impacts psychosocial well-being, from feelings of isolation and embarrassment to the onset of psychotic episodes and suicidality. To help patients cope with chronic and/or terminal symptoms, many healthcare providers advocate the benefits of social support groups as a palliative measure, such as weekly meetings of cancer patients. The rapid proliferation of such programs, as well as associated “survivor groups,” has attracted research attention, and the number of research studies on the topic is now substantial. The field would currently benefit from a quantitative synthesis of the extant findings. To systematically quantify the effect of such intervention groups on mortality (i.e., death rates), we undertook a meta-analysis of the research literature, comparing patient survival rates as a function of social interventions. Across 27 studies examined thus far, we encountered a surprising variability of intervention success in prolonging life. Although most intervention provided demonstrated evidence of increased survival rates among participants, several studies did not. Our presentation will discuss the implications of our findings for healthcare providers and pose questions that will need to be addressed in future research.

Rhett Thomson, Utah Valley University Health Previous studies have shown high-frequency (HF) ultrasound may be sufficiently sensitive to detect and differentiate between both the histopathology and molecular subtypes of breast cancer. The goal of this study was to test the boundaries of HF ultrasonic testing methods. The parameter of interest was the effect of formalin fixation on tissue samples. Formalin has been found to stiffen tissues. Since ultrasound is sensitive to histological properties such as stiffness and adhesion factors, stiffening caused by fixation may compromise the results of the HF ultrasound analysis. This study monitored potential changes to HF measurements caused by tissue fixation. The fixation solution used was 10% neutral buffered formalin. The same formalin solution is used by hospitals after breast conserving surgery (BCS) to prepare and preserve excised tissue specimens for pathology. If no change in HF ultrasound analysis is detected in tissue samples before or after fixation, prepared tissue specimens from BCS patients may be used as viable samples for HF ultrasonic testing. Twenty tissue samples were used in this study. All samples were excised from the cortex, medulla, or ureter of a fresh cow kidney. Samples were tested with HF ultrasound. Testing conditions and procedures were identical to previous breast cancer tests. HF ultrasonic testing was completed at the zero hour mark and then repeated at five successive time intervals during fixation. Data collected from each sample was compared across all time intervals to detect any changes caused by fixation. Analysis of the data showed no significant changes in HF readings before, during, or after fixation up to 17 hours. Any variations observed were within experimental error. These findings demonstrate that formalin fixation does not affect the accuracy of HF ultrasonic testing techniques on bovine tissue samples. To reinforce the findings, this study will be repeated using different types of tissues.

Jessica Russell, Dixie State University Fine Arts Early sources tell us women have traditionally played a background role in any event. It is only in recent decades that an interest in their historical role has taken place, and the field of musicology is no exception. Performers and composers that were well-known in their time have been forgotten as time moved forward. One such artist includes the Austrian composer and performer, Maria Theresia von Paradis. A contemporary of Mozart, Paradis was a traveling concert pianist and composer who is mostly remembered for being blind (Neuls-Bates 1982). Unfortunately, one of her most significant contributions to the field of music, namely her school of music for girls, has been forgotten and is left out of historical accounts almost completely. This school, which taught piano, voice, and music theory to girls, was innovative for its time (Fürst 2005). In this presentation, I will discuss the literature related to women in music in an effort to determine the extent to which these sources address Maria Theresia von Paradis and her contributions as a musician, composer, and pedagogue.

Natalie Jarvis, Brigham Young University Fine Arts My fascination with the process of distortion and my desire to bond with the transformative nature of the ceramic medium drives my exploration of its abilities and limitations. Making myself a part of the natural movement of the clay and helping each piece to find its abstracted balance is important during the creation process.

Rebekah Jackson, Brigham Young University Fine Arts This project has been an involved approach to studying the history of costume design, both through conventional research and the more tactile construction process. While Edith Head is a renowned costume designer with many books and articles on her life work, less has been done to understand how her designs functioned and evolved from concept to completion. But this process is essential to understanding her career’s success, as it opens valuable insights to how Head thought as a designer. It reveals what she was and wasn’t willing to compromise on, how she balanced aesthetics with practical considerations and how she worked with an actor or actress to achieve the proper look for their character. In Blake Edward’s production The Great Race, Edith Head’s designs showed this transformation of idea to reality by compromises between the original designs and finished garment. A simple red suit, worn in this production, exemplifies such compromise and was the construction portion of my research. Recreating this costume, in conjunction with conventional research on Head and her work, revealed important decisions Head made from materials to fit, how and why she changed her original design and the general approach she used in her work. These insights combine to form the basic pattern used in her creative process, applicable to both costume design and related fields as it unveils the thought process of one of Hollywood’s most successful career women.

Hannah Braegger McKeachnie, Utah Valley University Fine Arts Purpose

Corey Wolf, Westminster College Life Sciences The yeast species Saccharomyces cerevisiae is used in numerous industries including brewing, baking, and winemaking. In recent years an increased demand for low ethanol beers has pushed breweries to develop a beer that is both rich in flavor and low in alcohol content. The current strategies employed to decrease the ethanol concentration in beer are costly, time intensive, and result in less flavorful beers. In this study, a method for decreasing alcohol content in the brewing process through the use of metabolic inhibitors of Alcohol Dehydrogenase is tested. Alcohol Dehydrogenase is the enzyme responsible for the conversion of acetaldehyde, an intermediate in the metabolic pathway of yeast, into ethanol. Two competitive inhibitors of Alcohol Dehydrogenase, Pyrazole and Fomepizole, were applied to small scale fermentations in varying concentrations in an attempt to decrease the overall ethanol yield of the beer. The application of either inhibitor resulted in an initial decreased rate of fermentation overall, measured as a change of sugar and ethanol concentration. In the later stages of fermentation, the rate of ethanol production returned to normal, suggesting the inhibitors were either catalyzed or removed by the cell. While this method for manipulating the products of fermentation did not yield the desired effects, this study did highlight the importance of the fermentation pathway for maintaing healthy Saccharomyces cerevisiae cultures. This research may be helpful in understanding the complete metabolic pathway of brewer’s yeast, with further application to organisms with conserved pathways.

Kyle Hansen, University of Utah Life Sciences Our brain processes physical stimuli from the world around us to shape how we perceive our environment. In the olfactory system, this processing begins with olfactory sensory neurons that relay information about distinct odorants to a region of the brain called the olfactory bulb. Olfactory sensory neurons express the same odorant receptors converge onto structures within the olfactory bulb called glomeruli. Here, we map lateral interactions between glomeruli to better understand how olfactory information from the environment is processed within the olfactory bulb. Using GCaMP6, a fluorescent calcium indicator, in awake mice we can observe optogenetically-evoked patterns of lateral connectivity between glomeruli in the olfactory bulb. The findings from this study will help clarify potential interglomerular connection schemes and provide understanding of how local circuitry in the olfactory bulb transforms the representations of sensory information before it is relayed to higher brain areas.

Merrill Christensen, Brigham Young University Life Sciences Selenium (Se) has established chemopreventive efficacy against prostate cancer, the second leading cause of cancer death among men in the US. Currently, most studies only employ one single chemical form of Se, even though different forms of Se act through varied mechanisms to achieve their anticancer effects. In this study, we propose that systematically combining multiple forms of Se will produce an optimal combination of Se compounds to inhibit in vitro prostate cancer cell growth. As proof of principle, we (1) synthesized Se nanoparticles (nano Se), (2) determined the IC50s of methylseleninic acid (MSA), sodium selenite, and nano Se in PC-3 cells, (3) utilized mixture designs and response surface methodology to direct our combination experiments with these three compounds, (4) tested the fifteen combinations determined in the previous step, and (5) constructed a polynomial model to derive the optimum combination of MSA, sodium selenite, and nano Se to inhibit PC-3 cell growth. Our results showed that combining different forms of Se compounds enhanced its chemopreventive effect. Future studies will expand the use of three Se-containing compounds to four and demonstrate similar effects in xenograft mice.

Chelsea Moody, Dixie State University Life Sciences A relatively new pathogen, chytrid fungus, has been a major cause of decline in amphibians worldwide. This pathogen has been found on Canyon Tree Frogs in Zion National Park. Earlier data suggested that chytrid did not affect populations of Canyon Tree Frogs in Zion National Park. We predicted that since the populations weren’t impacted by the presence of the fungus, there must exist a mechanism by which the frogs rid themselves of the infective agent. To test this prediction, we captured and swabbed ten frogs in each of nine canyons in Zion National Park during the summer of 2013. Swabs were sent to the San Diego Zoo to be analyzed for the presence of chytrid DNA. Chytrid infection rates and population sizes were compared with data from previous years. As expected, the data showed that two of the three infected canyons experienced declines in the percentage of frogs testing positive for the fungus. Thus, these frogs must possess a mechanism to survive this pathogen, whereas most amphibians do not. Our results may prove helpful to other biologists seeking to understand how amphibians can survive this pathogen.

Eric Swenson, Dixie State University Life Sciences Underwater invasive species such as mussels and algae attach to submersible substrates that are advantageous for growth, and often include recreational boats that are frequently moved from one waterway to another. This can lead to rapid and uncontrolled spread of the invasive species. Through the use of newly developed ionic surfactants, growth and attachment of species on submersible surfaces can be inhibited. New surfactants have been incorporated into the final coating of typical product sealants to prevent the attachment of various marine organisms. By integrating the surfactant into the coating, an increase in the usefulness and longevity of effective inhibition is expected, as compared to commercially available alkali anti-fowling agents that are applied after the sealing process. It is hypothesized that the adherence properties possessed by marine organisms will be compromised by the introduction of ionic surfactants through changes in the chemical makeup of the exposed surfaces. The ultimate goal is to develop a substrate surface in which there is no affinity for attachment and/or causes fatality to the organism. If the desired outcome is reached, the potential benefits that ensue could create drastic improvements in areas of invasive species prevention and management in the desert southwest fresh-water lakes. Additionally, extension of the surfactant application to metal surfaces could have significant impacts on efficiency in water treatment systems, as well as cargo shipping in saltwater environments.

David Baird, Utah Sate University Life Sciences We obtained from the Bureau of Land Management (BLM) 30 years of monthly waterfowl population surveys completed at Pariette Wetlands in the Uintah Basin, Utah between 1980 and 2010. The Pariette Wetlands are the largest wetlands managed by the BLM within the lower-48 states and are comprised of 4,033 acres of land. Pariette Wetlands is surrounded by about 6,000 square miles of land where oil and gas production is the major activity. This waterfowl refuge is a significant location for migrating waterfowl species within the Pacific Flyway and provides important summer habitat for resident waterfowl. Our objectives were to determine what the trends were for waterfowl population abundance, occupancy, and species richness.

Sharisa Nay, Brigham Young University Life Sciences Gene therapy is a growing field of science with the potential to improve thousands of lives. With an eye toward improving the effectiveness and longevity of gene therapies, my project examines the preferential binding tendencies of the histone protein variants Htz1 and Htas1. Htz1 is the Ceanorhabditis elegans homolog of H2AZ, an important variant of the H2A histone. This protein has been shown as necessary for survival and as playing a role in the prevention of ectopic heterochromatin spread. Htas1 is another variant of H2A that plays a role in the increased transcription of sperm-producing genes. The preferred positioning of these variants on naked DNA is not yet known. Through a DNA extraction, nucleosome reconstitution, and DNA digest and sequencing, we will take these variants and examine where they are prone to localize within the N2 Bristol strain of c. elegans. This will allow us to include DNA sequences on our gene insertions possessing a high binding-affinity for these transcription-promoting histones. Thus, if we can identify the locations at which these variants will localize within DNA, we will be able to insert these preferred constructs into the genes used for gene therapy and thereby increase the effectiveness of gene therapies.

Joe Passman, University of Utah Life Sciences Neurodegenerative disorders and amyloidosis are thought to be a consequence of the misfolding of intrinsically disordered proteins (IDPs). In non-pathogenic states, IDPs are thought to manipulate their configurational ensembles (CEs) – through partially folding and/or forming secondary structure – to create binding sites for the multiple interaction partners necessary for cell-signaling, recognition, and regulation. An obstacle to accurate in vivo understanding of non-pathogenic mechanisms is that little is known about the impact of the crowded cellular environment on the global (i.e. partial folding) and local structural characteristics (i.e. secondary structure) of IDPs. It is difficult to understand why an IDP may misfold if little atomistic structural understanding exists regarding IDP functional mechanisms in non-adherent physiological states.

Shwan Javdan, University of Utah Life Sciences Each year in the United States, over 22,000 new cases of ovarian carcinoma are diagnosed and 14,000 women die from its progressive cancer stages. Anthracyclines, a class of common chemotherapy drugs, have long been the primary treatment for this and many other cancers, but they often leave patients with cardiotoxicity, hepatotoxicity, and other adverse effects. Polymer-drug conjugates using poly[N-(2-hydroxypropyl)methacrylamide] (poly-HPMA) are nanosized, water-soluble constructs that accumulate passively in solid tumors by the enhanced permeability and retention effect as well as actively by cell surface targeting methods. As such, they have exhibited reduced toxicity in the body. The goal of this study was to develop a novel, targeted HPMA copolymer-drug conjugate for the treatment of ovarian carcinoma. To accomplish this, an antibody fragment called Fab’, targeted to ovarian carcinoma cell surface antigen OA3, was bound to an HPMA copolymer-gemcitabine conjugate. Gemcitabine is a nucleoside analog used in chemotherapy that has demonstrated considerable effectiveness in recent years. The copolymer-gemcitabine conjugate was successfully developed following the synthesis of all the requisite components, including Fab’, diblock chain-transfer agent (di-CTA), N-(2-(2-pyridyldithio)ethyl)methacrylamide (PDTEMA), and polymerizable backbone-degradable gemcitabine derivative. Syntheses of di-CTA and PDTEMA were optimized and then characterized by high-performance liquid chromatography, H-NMR, and mass spectrometry. Monomers were combined by reversible addition-fragmentation chain-transfer polymerization, and then the Fab’ fragment was bound to the polymer backbone via a disulfide exchange reaction with PDTEMA. The targeted copolymer-drug conjugate was then verified by fast-protein liquid chromatography as well as ultraviolet-visible spectroscopy. A number of in vitro tests have demonstrated the properties of an efficient drug delivery system. Flow cytometry displayed active accumulation of the copolymer-gemcitabine conjugate to OVCAR3 cell surfaces. Furthermore, IC50 measurement found promising drug efficacy comparable to modern anthracyclines. Future work will involve in vivo evaluation of the conjugate’s therapeutic efficacy in nude mice bearing OVCAR3-xenografts.

Chase Barker, Utah Valley University Life Sciences Central Research Question: Phylogenetic relationships of mayflies are still not very well known, however molecular and morphological data have begun to shed light on the relationships of these insects (Ogden et al. 2009). Our central question is to elucidate the phylogenetic relationships within the mayfly family Baetidae.

Julia Hull, Weber State University Life Sciences Endophytes are fungi that live within aerial portions of plants for most or all of their life cycle without causing visible signs of disease. Gall forming aphids, Pemphigus betae, are highly competitive over gall site selection (Moran 1993), forming galls on the leaves of narrowleaf cottonwood and their hybrids. The favored gall location overlaps with areas of highest endophyte probability. I hypothesized that a negative correlation would exist between endophyte infection and aphid galling on leaves of backcross hybrid cottonwood trees.

Brianne Palmer, Utah State University Life Sciences Inversions have plagued the valleys in Utah resulting in built up pollution carpeting the cities and spreading into surrounding ecosystems. The ecological impact of these inversions is unknown. Inversion-based pollution events deposit nutrients and pollutants in the ecosystems. The purpose of this study is to determine if lichens, due to their unique physiology, preserve a record of inversion-based nitrogen deposition in order to assess the ecological impact of the inversions. In June and July of 2013 we collected 111 samples of lichens from sites exposed to the inversions, along Red Butte Creek and on an elevation gradient on Grandeur Peak in Salt Lake City. The collection focused on two species of lichens (Xanthomendoza montana and Xanthomendoza fallax) because they are easily identifiable and live in all the sampling sites. The samples were ground, run through a stable-isotope mass spectrometer and analyzed for the %N and δ15N. If lichens hold a record of inversion-based depositions there should be more nitrogen in lichens from the sampling sites closer to the pollution source (Salt Lake City). Once the data was complied, the resulting graphs showed no correlation between %N and distance from Salt Lake City between δ15N and the pollutant source. However, the isotopic variability within the samples was greater than expected and requires further research.

Preston Manwill, Southern Utah University Life Sciences Plants produce compounds, secondary metabolites, which can be harnessed for medicinal uses. Caryophyllene, a secondary metabolite produced by a variety of higher plants, has shown promise as an analgesic and anti-inflammatory. Caryophyllene synthase is the final enzyme in the biosynthetic pathway that produces caryophyllene. The gene and enzyme responsible for caryophyllene production have been observed in crop plants and model organisms, but few studies have investigated caryophyllene production in indigenous plants. We investigated the production of caryophyllene in Ericameria linearifolia (Narrowleaf goldenbush) because it is a shrub native to southwest North America. The first portion of this research examined sequence similarities between caryophyllene synthase gene orthologs in E. linearifolia and other plant genera. Genomic DNA was isolated from E. linearifolia leaves collected from plants growing in southern Utah. Literature reviews and database inquiries have provided DNA sequences for similar synthases, from which primers will be designed to copy and sequence the E. linearifolia caryophyllene synthase gene, confirming the orthologs presence. Following confirmation of the gene, mRNA will be extracted from leaf material and oligo(dT) primers used to synthesize cDNA. Subsequent cDNA amplification and cloning of products into a vector will allow for transformation into a protein-producing bacterium. These final steps make the downstream procedures of protein production, structure determination, and functional characterization of the protein possible. Comparison with enzymes that produce similar organic compounds may result as well, and would provide insight that could improve the biosynthesis of medicinal compounds.

Preston Manwill, Sourthern Utah University Life Sciences Plants produce myriad secondary metabolites (essential oil) that play a role in ecological interactions. Species evolve unique mixtures of organic compounds due to strong selective pressures that act on metabolic pathways. Few studies have investigated the antimicrobial properties of secondary metabolites that evolved against soil microbes associated with native plants. The essential oil of Mojave goldenbush (Ericameria linearifolia), a shrub native to the Intermountain West, was characterized and its bacteriostatic abilities against root associated and non-root associated bacteria morphs were examined in this study. We hypothesized E. linearifolia oil would act as a greater growth inhibitor against native soil bacteria with which it has evolved than against bacteria from non-associated soils. Secondary metabolites were extracted using steam distillation and analyzed with gas chromatography mass spectroscopy. A Kirby-Bauer assay tested presence, size and retention of inhibition zones (IZ) produced against soil bacteria. The essential oil of E. linearifolia contained twenty secondary metabolites, two of which, limonene and sabinene, constituted greater than fifty percent of the oil. Presence and retention of IZ produced by E. linearifolia metabolites varied significantly by community association and bacteria morph. The hypothesis was supported by IZ that were largest against bacteria cultures isolated from soil that surrounded Mojave goldenbush roots and least effective against cultures isolated from non-root associated soil. Our findings suggest that secondary metabolites of E. linearifolia have evolved to specifically prevent negative interactions with bacteria most associated with the plant.

Cameron Sargent, Brigham Young University Life Sciences Although viruses present a potential solution for treating and studying many harmful diseases, they have yet to be utilized successfully, largely due to an overall lack of understanding of the interactions between viruses and their hosts. This study, however, demonstrates the efficacious usage of comparative genomics in further elucidating viral reproduction mechanisms, a step in overcoming this hurdle. Following the sequencing and annotation of genomes of the B4 subcluster of mycobacteriophage, a previously unstudied selection of viruses, comparative genomics analysis identified regions of proteins from these viruses that are highly conserved, or highly similar. One of these B4 phage proteins was then compared on the basis of its predicted folded structure and its amino acid composition to a protein with the same function from another phage, whose structure and DNA binding mechanism was determined via NMR spectrometry. Gene sequence alignments identified the region of highest conservation in the B4 phage. Protein folding then revealed that this region created a structure that was analogous to the functional region of the protein studied elsewhere, indicating that it too recognizes, binds to, and modifies DNA in the same manner. Furthermore, another DNA-binding motif with high conservation among B4 phage was observed in both the folded B4 proteins and the protein analyzed via NMR spectrometry. This comparison procedure not only further elucidated the function of an important gene in B4 phage but also displayed that comparative genomics, a resource-effective and computer-based approach, can successfully identify the critical functional regions of a protein. This study therefore provides a novel procedure for further understanding the mechanisms of virus-host interactions, which in turn facilitates the successful application of viruses in treating and studying diseases.

Eliza Warren, Brigham Young University Life Sciences We have previously demonstrated that ethanol inhibition of medium spiny neurons (MSNs) in the nucleus accumbens (NAc), and γ-aminobutyric acid (GABA) neurons in other ethanol-sensitive brain areas, is mediated by GABA(B) receptors. The aim of this study was to evaluate the involvement of GABA and glutamate (GLU), and in particular GABA(A), GABA(B) and NMDA receptors, in mediating ethanol inhibition of dopamine (DA) release in the NAc. Using fast scan cyclic voltammetry (FSCV), we evaluated the effects of ethanol on DA release in the NAc core of C57/BL6 and CD-1 mice. In the slice preparation, local stimulation evoked robust, frequency-dependent DA release in the NAc, with maximal release at 20 Hz. Ethanol decreased DA release with an IC50 of 60 mM in C57Bl6 mice. In anesthetized C57BL6 mice, ethanol decreased DA release with an IC50 of 2.0 g/kg. Superfusion of the GABA(B) receptor antagonist CGP55845 enhanced DA release 85%, while neither the GABA(A) receptor antagonist bicuculline, the GABA(A) receptor agonist muscimol, the GABA(B) receptor agonist baclofen, nor the NMDA GLU antagonist APV significantly affected DA release. CGP55845 blocked ethanol inhibition of DA release, while neither bicuculline nor APV altered ethanol’s effects. GABA inhibited DA release at 0.5-10 uM but not at 0.1-1 mM. Compared to ethanol effects on DA release in C57BL6 mice, CD-1 GAD GFP knock-in mice, which only express GAD at 50% levels, were significantly less sensitive to ethanol. As DA release was sensitive to low concentrations of GABA, CGP55845 may act as a GABA(A) rho receptor antagonist, and neither bicuculline nor baclofen had any effect on DA release, ethanol may be acting on extrasynaptic GABA(A) rho receptors on DA terminals to inhibit DA release in the NAc.

Ryan Watkins, Univeristy of Utah Life Sciences The lymphatic system is responsible for controlling systemic fluid buildup. Lymphangiogenesis is a dynamic process involving sprouting and maintaining new lymphatic vasculature. Vascular endothelial growth factor C (VEGF-C) is known as a key growth factor through VEGF receptor 2 and 3 (VEGFR2 and VEGFR3). Interestingly, the cornea expresses VEGF-C but is alymphatic. We found that the soluble isoform of VEGFR3, which lacks tyrosine kinase domains, is responsible for the alymphatic nature of the cornea by sequestering endogenous VEGF-C. Although soluble VEGFR3 or soluble VEGFR2 can be useful for inhibition of VEGF-C derived lymphangiogenesis, they also bind VEGF-A. Inhibition of VEGF-A suppresses blood vessel formation, damaging tissue and creating additional side effects. The development of a new anti-lymphangiogenic drug, that only blocks VEGF-C, has many implications: preventing tumor metastasis and reducing rejection rates of tissue and organ transplants. VEGF-C mainly binds to VEGFR3 domain 2 and VEGFR2 domain 3. To develop an anti-lymphangiogenic drug that specifically binds VEGF-C the binding domains were inserted into a vector that produces a recombinant protein (VEGF-C trap) that sequesters VEGF-C, suppressing lymphangiogenesis. Mice in a cornea transplantation model were treated with VEGF-C trap. After 8 weeks, 60% of the treated cornea survived (no rejection) compared to 10% in the empty vector control group. A 60% transplant survival rate is one of the highest rates compared to other single treatment methods. Blood and lymph vessel area was calculated and showed a decrease in lymph vasculature but not blood vessel. Suggesting only VEGF-C activity was affected. In a tumor metastasis model, nude mice will be injected subcutaneously with cultured MCF-7 cells which have been transfected with VEGF-C trap or an empty vector. We expect to see a decrease of lymphatic vasculature in the mice injected with MCF-7 cells containing the VEGF-C trap and ultimately less metastasis.

Kevin Nay, Southern Utah University Life Sciences Leech taxonomy has traditionally been based on morphological characters, but with new developments in DNA technology many taxonomists are starting to use genetic information in descriptions of new species. Leeches in southern Utah are poorly inventoried with respect to many other aquatic animals. There have been few morphological inventories of leeches and even fewer descriptions of the genetic diversity within leeches. Landscape genetics is a powerful tool used to understand geographic patterns of genetic diversity. Southern Utah has many naturally isolated bodies of water due to the climate and the dramatic changes in elevation in this part of the country. The landscape genetic study of leeches in southern Utah will provide us with a better understanding of genetic differentiation within southern Utah leeches. The mitochondrial DNA (CO I region) will be used to estimate genetic diversity and examine the relationships among individuals in two populations of leeches. I hypothesize that leeches in southern Utah will have greater genetic diversity then historically recognized from morphological studies suggesting a new species of leech. The study will lead to better understanding of the taxonomy and identification of southern Utah leeches.

Preston Manwill, Southern Utah University Life Sciences Members of Anthoxanthum (sweetgrass) have a history of ceremonial and medicinal use. Coumarin, a secondary metabolite produced by the grass, is an anticoagulant and antimicrobial agent. Antimicrobial properties of commercially available European A. odoratum metabolites have been nominally investigated, but no North American species have been studied. Additionally, European sweetgrass is purported to be available in both diploid and polyploid strains. Our research objectives were to: 1) determine if ploidy level strains do exist, and 2) investigate secondary metabolite production and evolution in commercial strains and A. hirtum, a Great Basin native. Using epidermal casts and fuschin staining, differences in guard cells and nuclei were surveyed. Secondary metabolites from the A. odoratum strains and A. hirtum were obtained through steam distillation and a vacufugation protocol that concentrated hydrosols. GC/MS analyses characterized and quantified secondary metabolites. The secondary metabolites coumarin, dihydrobenzofuron, and dihydroactinidiolide were identified, with the first and latter greatest in A. hirtum. Significant guard cell differences between strains, as well as species, were observed. Two ploidy strains of A. odoratum were suggested. A Kirby-Bauer assay tested presence, size and retention of inhibition zones (IZ) produced against soil bacteria. Gram-staining initially characterized bacterial morphs. Secondary metabolites from the diploid strain were most effective against all bacterial morphs, but polyploid metabolites also generated and retained IZ against diploid root associated and non-root associated morphs. The native sweetgrass produced IZ only against root associated bacteria with which it had evolved. Future work will include similar studies of other North American Anthoxanthum species.

Justen Despain, Brigham Young University Life Sciences Mycobacterium ulcerans is an acid fast intracellular bacteria that is the causative agent of Buruli Ulcer, a disease endemic in tropical regions of Africa. The disease starts as a nodule that progresses into a necrotizing skin lesion. Treatment ranges from antibiotic therapy to surgical removal of surrounding skin to stop necrotization of the skin. Owing to the intracellular nature of M. ulcerans, antibiotic therapy can be an ineffective treatment option. It has been previously shown that mycobacteriophage can reduce extracellular bacterial load in mice infected with M. ulcerans. In this study, we propose a method of killing intracellular M. ulcerans by mycobacteriophage using avirulent Mycobacterium smegmaits as a vector.

Brady Evans, Brigham Young University Life Sciences Nutrient-sensing kinases, such as AMPK and mTOR, play a key role in regulating cellular metabolism. They recognize nutrient levels within the cell and distribute nutrients accordingly. The failure to coordinate metabolic processes can lead to diseases such as diabetes, obesity and cancer. PAS kinase is another member of the nutrient-sensing kinase group that regulates glucose distribution in the cell. Despite its clear importance, little is known about the mechanisms regarding PAS kinase activation and its function. Studies have shown that mice without the PAS kinase gene are resistant to weight gain and maintain insulin sensitivity when placed on a high fat diet. Weight gain and insulin resistance are associated with increased rates of diabetes, cancer and other metabolic diseases. AMPK is currently the key target for the treatment of diabetes and has been found to be a cancer target as well. The yeast homologue, Snf1, is necessary for activation of yeast PAS kinase. Further studies have shown that PAS kinase is activated under the same respiratory conditions as Snf1. The aims of this project is to determine if Snf1 directly phosphorylates PAS kinase and to determine the effects of this phosphorylation. We have found that PAS kinase is activated quickly when cells are placed under Snf1-activating conditions. In addition, Snf1 is necessary and sufficient for this activation. Snf1 also copurifies with PAS kinase suggesting this activation is direct. PAS kinase purified from wild type verses Snf1-deficient cells shows phosphorylation on two key residues, S1020 and S1035. In addition, Snf1 directly phosphorylates PAS kinase in vitro. Together these findings suggest that Snf1 directly phosphorylates PAS kinase in order to regulate cellular glucose homeostasis

Bruce Chiarelli, Brigham Young University Mathematical Sciences A Gallai coloring of the complete graph on n vertices is an edge coloring such that no triangle has edges of three different colors.

Samantha Smiley, Brigham Young University Mathematical Sciences Dementia is a clinical syndrome characterized by an overall loss of cognitive ability. There are multiple forms of dementia with various causes and various impacts on the suffering individuals. Accurate diagnosis is essential to effective intervention and treatment. Currently, clinicians lack a biological marker that definitively distinguishes the different forms of dementia. Hence, they rely on physical exams, neuropsychological tests, and patient report to provide a diagnosis. Recent advances in brain imaging make it possible to obtain detailed maps of brain activity, which in turn may offer insight into many conditions such as dementia. Developing a predictive model from patient data, including brain scans, would greatly enhance the ability of clinicians to provide accurate diagnosis, and hence appropriate treatment, to their patients. Doing so, however, is not trivial as patient data is heterogeneously and non-uniformly distributed across sites, where some sites have far more data than others and calibration varies among scanners used. We report on the development of novel predictive models based on distributed learning for the effective diagnosis of dementia.

Steffan Larsen, Brigham Young University Mathematical Sciences The popularity of metamaterials has exploded with in the last decade. Metamaterials are materials that exhibit interesting properties not found in nature; one of the most widely known features being a negative refractive index. Metamaterials are composites different types of materials that give them their interesting properties. In addition to being composed of several element types, metamaterials also contain certain inclusions that influence their electromagnetic properties. Among these are space filling curves. Space filling curves are curves that are entirely contained within a specific area and yet can become infinitely long. In my research I investigated the properties of space filling curves and their application/benefit to the research surrounding metamaterials, specifically metamaterial antennas.

Brennon Bauer, Southern Utah University Mathematical Sciences We study the Lotka-Volterra competition model. This model can be applied to Math Biology. We propose a Semi-Implicit numerical scheme, which guarantees that the populations are always positive. Also we prove that the numerical scheme is uniquely solvable, and is unconditionally stable.

Ariel Herbert-Voss, University of Utah Mathematical Sciences A typical problem in numerical analysis is finding a smooth interpolation of a given data set such that information at extended positions can be evaluated. When extended to higher dimensions, there are few such algorithms available for practical use. Drawing from techniques used in geometric modeling we developed a practical algorithm with improved complexity by implementing the techniques in a query model as part of a MATLAB software package. From initial input data the algorithm builds a d-dimensional cell complex using Delaunay triangulation. Each cell has an associated interpolation function that satisfies Lipschitz continuity for each new point. During query time the user specifies a query point and the algorithm returns the interpolated function value. To reduce complexity related to point location within the cell complex, we implemented a binary tree search based on hyperplane decision criteria. Efficiency analysis completed using benchmark data sets indicated that the decision tree algorithm improved the efficiency from O(N) to O(N log N). This algorithm is the first of its kind that can be used on actual data sets and is the first implemented as a MATLAB package.

Nirdosh Chapagain, Brigham Young University Mathematical Sciences Rainbow is an optical phenomenon created by reflection and refraction of light at the boundaries of water droplets. Descartes was the first to provide a geometric explanation for the optics of the rainbow. We use Descartes’ method to examine if rainbow effect is possible with metamaterial droplets. Metamaterials are artificial materials whose permittivity and permeability can be simultaneously negative hence, giving them negative index of refraction for certain frequencies. The recent extraordinary level of output in the field of metamaterials has resulted in examinations of applications of these substances to a variety of fields, including the arrow of time and cloaking. Many applications of metamaterials can create physical effects that were previously assumed impossible. In this study we have considered that our hypothetical droplet has negative refractive index for visible band of the electromagnetic spectrum. We also examine the effect of using composite metamaterial droplets.

Ammon Washburn, Brigham Young University Mathematical Sciences In many problems with seepage flow, there are non-linear problems that don’t have an easy analytical solution. There is already good research on what can be done in certain situations with these problems. I will present on numerical methods that have been proven to solve certain conditions and then present other solutions for similar problems where the numerical method isn’t so readily available in past research. I will implement the algorithms and compare results.