Engineering

Cameron Rogers, Brigham Young University Engineering The mechanical properties of materials are greatly influenced by their microstructure. Grain boundaries, part of the microstructure, effect mechanical properties and the manufacturing of crystalline solids. Grain boundaries in nickel have been shone to be more mobile at temperatures approaching the melting temperature (Olmsted, Holm, and Foiles, 2009). However, little is know about their behavior at low temperatures, and the notion that mobility decreases with decreasing temperature may be incomplete. Using the molecular dynamic simulator LAMMPS (Large-scale Atomic/Molecular Massively Parallel Simulator) we are simulating the mobility of 41 Σ3 boundaries in nickel, a face centered cubic (FCC) metal, at various cryogenic temperatures. We have begun to see that these boundaries defy the previously stated notion and move faster with decreasing temperature. Using these molecular simulations we are also investigating the underlying mechanisms for this phenomenon, which could lead to further investigation.

Neil Hinckley, Brigham Young University Engineering Due to the increasing interest in combining the physical and digital world devices which allow users to naturally interact with digital systems are becoming much more important and prevalent. In order to improve upon standard haptic controllers and interfaces we explored compliant haptic devices, which us a compliant member to provide tunable force feedback to users. We were able to produce a prototype device and demonstrate some of the capabilities and advantages of compliant haptic devices.

Ryan Putman, Utah State University Engineering Spider silk possesses superior mechanical properties to most other biological or man-made materials. In particular, research has demonstrated that spider silk is as strong as Kevlar, yet much more elastic. The unique feature of both strength and elasticity naturally piques interest in numerous scientific fields (e.g. medical sutures, automobile seat belts, or athletic performance wear). However, the limiting factors in using spider silk on a commercial scale are the production of sufficient protein product and the ability to do so in a cost-effective manner. An additional challenge is due to the territorial and cannibalistic nature of spiders, which makes harvesting their silk from large “spider farms” an infeasible task. To overcome these limitations, an approach using synthetic biological engineering principles has been employed. This emerging field of study provides a powerful tool, the use of standard biological parts called BioBricks. Using these standard DNA parts, a genetic circuit with the necessary regulatory components was engineered to recombinantly produce a synthetic spider silk protein in the microorganism Escherichia coli, which will provide a more consistent and sustainable source of spider silk than by harvesting directly from spiders. Expression of this recombinant protein has been verified through SDS-PAGE protein gels and subsequent protein immunoblot. The next step is to create a genetic circuit that will be used to secrete the spider silk protein outside of the E. coli bacterium. This could greatly reduce the downstream processing costs associated with protein purification as well as potentially increase overall yield. Therefore, a genetic tag that targets products for secretion has been fused to the spider silk coding regions. Further testing is required to determine the difference in overall protein yield from secreting as opposed to non-secreting strains of the engineered bacteria. Once these values are determined, the production can be optimized and scaled-up.

Brad Hancock, Brigham Young University Engineering Fischer-Tropsch (FT) synthesis is a reaction used to convert carbon monoxide and hydrogen into high-quality liquid fuels. The reaction takes place in the presence of a cobalt- or iron-based catalyst. An important factor in how well a catalyst works is how highly it is dispersed on a given support. Sugar can be used to increase the viscosity of the impregnation solution to change the dispersion of the cobalt on the alumina support. The present study will determine the effect of adding sugar to the impregnating solution and dispersion of cobalt on the alumina support.

Dayton Minore, Brigham Young University Engineering Microwave scatterometers, which use radar backscatter measurements from satellites to infer wind vectors near the ocean’s surface, have the ability to monitor global wind speeds at high resolutions. Such data is used for weather forecasting and climate research. However, scatterometer observations can be contaminated by land proximity. Consequently, current methods do not use measurements within 30 km of the coast (about 10.6 million square kilometers worldwide) in the data set. This unused data can be utilized by a recently developed algorithm that can measure winds as close as 5 km to the coast. The author proposes that areas near land can be systematically targeted for special processing, providing valuable near coastal wind data. To demonstrate the effectiveness of the targeting method on a global scale, a sample 4-day data set will be processed. The data is to be stored and published in compatible file formats to current wind data, so that it will be easily usable by wind-vector users.

Jeremy Hunt, Brigham Young University Engineering Unnatural amino acid incorporation is a power tool in the synthetic biology toolbox that allows for unique residue chemistry to be incorporated into proteins. This technology has many promising applications in areas such as protein-protein interaction, biotherapeutics, biosensing, and biocatalysis. One challenge of uAA-incorporation is the current inability to properly predict the impact of the novel uAA-residue chemistry on proper protein folding and function. Therefore, a screening technology would be desirable to rapidly assess the viability of uAA-incorporation sites. Cell-free protein synthesis (CFPS) provides a promising basis for rapid screening technologies. The open environment of CFPS has a variety of advantages over conventional in vivo systems, such as direct monitoring, selective protein expression, and facile deployment of synthetic pathways. Another primary benefit CFPS has over in vivo expression is the ability to directly express proteins from PCR-generated products, known as linear expression templates (LETs). The use of LETs decreases the labor and time to expression of recombinant proteins. Thus, LET-based CFPS is a propitious system for rapid screening of uAA-incorporation. Here we demonstrate uAA-incorporation using LET-based CFPS and identify how this technology can significantly reduce time and labor to rapidly express proteins containing uAAs.

Christopher Hutchings, Brigham Young University Engineering The biocatalysis industry has been rapidly expanding due to the fact that there has been a greater demand for ecologically friendly manufacturing processes. The benefit of biocatalytic systems is that it enables stereo-, chemo-, and regio- specificity in chemical manufacturing. This in turn reduces wasteful byproducts from chemical manufacturing. This is especially valuable in industries where removal of chemically similar but physically harmful waste products is essential. The problem with the traditional biocatalytic processes is that they are hindered from limitations in areas such as enzyme stability, leaching, recoverability, and reusability. These limitations significantly impede the cost-effectiveness of biocatalysis for industrial applications. The processes of enzyme immobilization like adsorption, entrapment, and other such forms of immobilizations provide improvements such as stability, recoverability, and reusability. Though they provide improvements they also go through enzyme leaching, complicated or even toxic conjugation procedures and have a lack of specificity to attachment location from. This ends in being counterintuitive and defeats the purpose of enzyme immobilization. It is here we start to build upon the recent advancements in unnatural amino acid and incorporating them into enzymes to demonstrate a biocompatible and covalent enzyme immobilization process that improves protein stability and enables attachment orientation control. This system we refer to as the Protein Residue-Explicit Covalent Immobilization for Stability Enhancement or PRECISE system, and it permits the covalent attachment of enzymes at potentially any location on the enzyme onto a surface. Using this process, we create reusable enzymes that are more stable and more resistant to harsh conditions. We have also concluded from this process that there is no leaching and increased stability from immobilization with the enzyme with satisfactory results in enzyme activity.

Bryce Ostler, Utah Valley University Engineering Much of SQL’s power derives from SQL’s declarative rather than procedural nature: a programmer describes the result desired rather than how to produce the result. Systems using SQL must translate SQL’s declarative language into a procedural language in order to execute queries. Relational Algebra (RA) is a procedural language that SQL can be transformed into and executed on a computer using a RA engine. Optimizations are applied to RA code to improve the performance of a translated query. The author of this abstract will present a simple RA engine written in Python and how it has been used as part of a Database Theory course.

Sean Bedingfield, Utah State University Engineering The delivery of zinc ions using ZnO nanoparticles within the body has been shown to cause the destruction of tumor cells and may also treat neurodegenerative disorders. The silane capping of ZnO nanoparticles is employed as a post-synthesis method to protect them from dissolution in polar solvents. Preliminary research demonstrates standard methods of silane capping result in aggregation of nanoparticles. Aggregation produces particles significantly larger than the original diameter of the nanoparticles, making them too large for some medical applications.

Jacob Robinson, Brigham Young University Mechanical Engineering Figure skating is a competitive sport that requires athletes to practice up to 5 days a week year round performing 50 to 100 jumps per day. This results in high, repetitive impact forces on the skater’s body which may lead to overuse injuries. While the negative effects of figure skating are well documented, the cause of these injuries is still unclear because the complexity of artistic figure skating limits current instrumentation from accurately measuring impact forces. This project has sought to fill this void by developing a force measurement system that will allow the figure skater to perform their jumps without any hindrance while accurately measuring the magnitude of the impact forces in the vertical and horizontal directions. Using strain gauges attached to the stanchions of the ice skate combined with a data collection system that attaches to the bottom of the boot, we have developed a prototype that accurately measures the forces produced in the ice skate. This will lead to a fully developed ice skate measurement system which will be used by researchers to investigate the impact forces generated in figure skating jumps and landings.

Derek Chang, University of Utah Bioengineering Atrial fibrillation (AF), the most common cardiac arrhythmia, is a rapid, irregular heart beat arising from uncontrolled and asynchronized electrical activation in the atria. This disruption of the normal electrical signaling hinders the contraction of the heart, leading to decreased blood flow, possible clot (thrombus) formation, and an increased risk of stroke. The left atrial appendage (LAA) is a small muscular pouch of highly variable anatomy within the left atrium. The LAA plays a prominent role in thrombus formation in patients with AF because of decreased blood flow within this structure. Thus, we hypothesized that the shape of the left atrial appendage is different in AF patients with a documented history of stroke. We used statistical shape analysis to determine which LAA shape variations contribute to stroke based on a cohort of AF patients who had both MRI and CT scans and a documented history of stroke. We manually delineated the boundaries of the LAA from each patient’s CT and MRI scans to analyze the resulting LAA segmentations for shape variations across imaging modalities and history of stroke. The results showed that patients who have AF and a history of stroke have an LAA with a narrower insertion site into the left atrium and are larger in size. In contrast, patients who have AF, but no history of stroke, have an LAA with a wider insertion site, which are smaller in size. By isolating specific LAA shape variants indicative of an underlying risk of stroke, we can use this shape classification scheme to better tailor AF therapies to each individual patient.

Autumn Pando, Brigham Young University Mechanical Engineering The wrist is one of the most common sites for joint injury. Over two-thirds of 75,000 annual repetitive joint injuries occur at the wrist. Excessive or abnormal wrist forces are thought to be one of the main contributing factors, yet no characterization of normal wrist forces exists. The purpose of this research is to fill this lack by creating a systematic, quantitative characterization of wrist forces and torques experienced in daily life. This database will aid further research in developing better and more personalized treatments as well as improving design considerations for human-machine interfaces. Ten healthy subjects participated in the experiment. Subjects performed 25 activities representative of daily life (e.g. hygiene maintenance, food preparation, using technology). Electromyographic (EMG) sensors recorded wrist muscle activity and electromagnetic motion sensors recorded wrist kinematics. Each subject performed a calibration task prior to the experimental protocol to determine the proportionality constant between EMG activity and torque. Wrist force and torque were determined from EMG activity using a constant of proportionality (identified by calibration), muscle length, and muscle velocity. Co-contraction was computed from torque. Wrist muscle usage, forces, torque magnitudes, torque angles, and percentage of co-contraction at varying levels of contraction were characterized. The results indicate muscle use, forces, and torques are unevenly distributed.

Kay (Beau) Freckleton, University of Utah Mechanical Engineering Current quad-rotors provide excellent maneuverability and opportunity for data collection in large scale areas such as agriculture, but lack the capability to maintain flight for an extended period of time. This is due primarily to a lack of energy supply, requiring operators to replace the battery source before the quad-rotor completes its designated purpose [1]. In this paper, we introduce a mechanism to enable flying robotic rotorcraft, such as quadrotors and helicopters which utilize vertical takeoff and landing, to perch similar to a bird. The mechanism is passive, using only the weight of the rotorcraft for actuation. Such a mechanism will dispel the need for multiple batteries by allowing the quadrotor to perch amid its designated flight course, recharge using solar energy, and complete data collection over large scale areas in less time. In previous research to this same end, biomimetic approaches were pursued, resulting in designs that looked similar to the legs and feet of birds. The design in this paper utilizes a Sarrus mechanism to convert rotorcraft weight into perch grip. We began by designing the mechanism for a range of cylindrical perch sizes. Using MATLAB, we developed a program to optimize the various mechanism dimensions needed to perch within this range while also maximizing the mechanical advantage of grip force. Using SolidWorks, a 3-dimensional model was created and manufactured with these dimensions. In testing, the mechanism successfully allowed a small RC helicopter to perch on a 4cm diameter rod with some resistance to external forces. However, in crash tests, the mechanism was unable to withstand certain moment forces and developed large fractures. Further research will focus on decreasing these moment forces’ ability to weaken the mechanism while optimizing perching capability.

Kimberly Uchida, University of Utah Bioengineering and Oncological Sciences Programmed cell death 4 (PDCD4) loses its function as a tumor suppressor when co-expressed with a specific binding partner, protein arginine methyltransferase-5 (PRMT5). A better understanding of the regulation of PDCD4-PRMT5 interaction may lead to cancer therapies targeted at restoring the tumor suppressive function of PDCD4. Using Xenopus laevis (frog) eggs to generate extract trapped in either interphase or mitosis, we found that PDCD4-PRMT5 interaction is regulated by the cell cycle. Full-length PDCD4 bound PRMT5 more robustly in interphase than in mitosis. However, a truncated version of PDCD4, that retained the PRMT5 binding site, bound PRMT5 equally in interphase and mitosis. These results indicate that there may be a component of full-length PDCD4 that occludes the PRMT5 binding site in mitosis. Furthermore, we found that both full-length and the truncated PDCD4 are preferentially phosphorylated in mitosis, but preferentially methylated in interphase. From these results we speculate that phosphorylation of PDCD4 in mitosis allows PDCD4 to fold upon itself and effectively block the PRMT5 binding site. Additionally, phosphorylation may prevent methylation even when PRMT5 can bind PDCD4, explaining the lack of PDCD4 methylation in mitosis. Such an inhibitory mechanism may be useful in therapeutically restoring the tumor suppressive function of PDCD4. Future research will be aimed towards completing our understanding of PDCD4-PRMT5 interaction in the cell cycle, such as proving our model in human cells.

Connor George, Utah State University Electrical and Computer Engineering The SABER instrument, aboard the TIMED satellite, measures optical data regarding parameters of the Earth’s atmosphere with respect to altitude. Approximately once per minute, SABER performs a limb-scan measurement on the Earth’s atmosphere from which altitude emission profiles of key atmospheric gasses, including hydroxyl at wavelengths of 1.6 μm and 2.0 μm, are derived. Most hydroxyl profiles within the SABER dataset contain a single peak in the airglow altitude profile centered near an altitude of 87 km, but a significant portion of the profiles display two or more local maxima. MATLAB code was written to analyze the geophysical and temporal global distribution of the multiple-peak profiles. Graphs have been created which display relationships between the percentage of multiple-peak profiles and the local time, the cardinal orientation of the SABER device, and the latitude and longitude at which the atmospheric profile was measured. Patterns have been observed in multiple-peak profile distribution with respect to these variables. Possible causes of the multiple-peak occurrences in the hydroxyl altitude profiles include waves, geometrical effects of the SABER instrument, and/or chemistry of the atmosphere. In addition to graphing software, analysis software was written which counts the number of peaks present in any given altitude profile, and which ascertained the percentage of profiles displaying multiple-peak characteristics. A small (<1%) portion of hydroxyl altitude profiles were found to have abnormal distributions due to erroneous or noisy data collected by SABER. Software has also been written to remove such exceptions from the dataset. Additional investigation into the relationship between multiple-peak occurrences and cardinal direction orientation of the SABER device is required in order to further identify the causes for multiple peak profiles. An investigation into seasonal patterns for multiple-peak profiles is to be conducted. As the dataset grows, exception software will be updated to identify invalid altitude profiles. Also, ozone has been found to have multiple-peak altitude profiles similar to those of hydroxyl, and studies complementary to those performed on hydroxyl altitude profiles will be performed on ozone.

Mitchell Dabling, Utah State University Civil and Environmental Engineering Water management and flood control are essential elements of civilization. Linear weirs (e.g. ogee crest, sharp crested, and broad crested weirs) are often used in irrigation channels or reservoir spillways to regulate the discharge and upstream water level during flood flows. As hydrologic data sets increase in size and accuracy, the highest probable maximum flood (PMF) discharge is becoming increasingly more accurate, and in many cases much larger than previous estimates. Because of this, an older weir may need to be rehabilitated to ensure it can pass the updated PMF discharge safely without upstream flooding. A nonlinear weir (e.g. labyrinth or piano key weir) can replace a linear weir in a channel or spillway to pass significantly more discharge without requiring increased channel width. The Utah Water Research Laboratory at Utah State University, with the help of Utah Mineral Lease Funds, has developed and published design data for multiple configurations of nonlinear weirs. In 2012, the Utah Division of Water Resources used this data to design a labyrinth-style nonlinear weir that will replace the spillway currently in use at Millsite Reservoir in Emery County. This rehabilitation project will significantly decrease the flooding potential of the surrounding area.

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