Architecture

Authors: Sarah Adams, Bailey Bennion, Sara S Rocks. Mentors: Sara S Rocks. Insitution: Utah Valley University. The abundance of microplastics within Earth’s environment is of great concern to public health. Heavy toxic metals like lead and mercury concentrate on the surfaces of microplastics, and can readily enter the biosphere. This poses a risk to humans and other forms of life because of the large amount of microplastics that have been found in even the most remote locations on Earth. Not much has been studied about the origins and presence of microplastics in Utah snow. It would be beneficial to study microplastics in snow because, once the snow melts in the spring, it is a substantial source of water for Utah and communities in the American West. Preliminary data indicates increased concentrations of microplastics in snow samples that originated from ski resorts compared to snow samples in areas with little recreation. The current project further investigates the origins and quantity of microplastics in the snow at the Sundance ski resort. Snow samples from the Sundance ski resort and from a remote location with little human activity are compared. Additionally, the number of microplastics at Sundance between natural and artificial snow samples are compared to see if the plastic components within snowmaking machines generate a greater amount of microplastics in the snow. To investigate if snowboards and skis can release plastics, microplastics in fresh snow are quantified before and after snowboard contact. Because microplastics can also be shed from clothing, researchers also examined samples for fibers generated from winter garments. This project aims to shed light on the sources and number of microplastics in Utah snow, especially the effects of skiing and snowboarding.

Authors: Matthew Drachman, Caden Weaver, Nicholas Martin. Mentors: Kal Munis. Insitution: Utah Valley University. There has been a lot of public debate in recent years when it comes to the topic of guns in the United States. Scholarship on the subject has shown varying opinions on how people believe on gun ownership, along with the subject of carrying firearms in public. A Pew Research study found 46% of people supported concealed carrying in places, while support among gun owners was 67% (Parker et al., 2017). While research has been conducted on concealed carry, research on open carry support and the factors that contribute to it are rather unknown. Within our study, we attempt to see how support for open carry, which is legal in most places in the U.S., is affected based on who is doing the open carrying of firearms. Particularly controversial political and racial groups. Within our model we randomly assign survey respondents to either our control question to gauge their support for open carry, or one of four vignettes we have designed to see if exposure to the treatment causes a change in how support for open carry will change. Our results have important implications pertaining to attitudinal (in)stability on core civil liberties within the United States.

Authors: David Schloesser. Mentors: B Kal Munis. Insitution: Utah Valley University. How do place-based identities influence American’s attitudes towards perpetrators of politically motivated violence? Through the implementation of a pre-registered conjoint experiment, I seek to answer this question. Ideally, and under normative circumstances, punishment of politically motivated violence should be applied to perpetrators with considerations only towards what/who was targeted in the act and the severity of the violence committed. In the U.S. however, evaluations of guilt are determined through the trial of peers, leaving room for the influence of in-group and out-group biases to appear in the judgement of perpetrators according to the suggestions of Social Identity Theory. As such, biases arise that cloud human judgement based on preconceived attitudes towards various group identities that influence our opinions when judging other individuals. One’s sense of place, or how strongly they identify themselves with their locality and its’ culture, is one of these powerful group social identities that establishes a salient “in-group” persona to other locals, while influencing attitudes towards various “out-groups” of people not from that place. Holding constant other considerations pertinent to acts of politically motivated violence, Americans more harshly evaluate perpetrators of violence from geographic communities other than their own. To judge the relative weight that these place-based identities hold towards the judgement of political violence perpetrators, evaluators were presented with perpetrators with randomly assigned personal characteristics (e.g., where they are from) and varied features of the violent act (e.g., how severe the act was, what/who was targeted), and asked to determine the level of punishment or mercy to be assigned to each perpetrator. I found that perpetrator place characteristics indeed influences the evaluator’s excusal of political violence, however when weighted against the features of the act, the nature of the act matters much more for evaluations on average. These findings can be interpreted as normatively negative, given that an evaluator’s place identity appears to reflect in-group out-group biases that affect the assignment of punishment to a crime. However, the disproportionate effect of the violent act’s target and severity are normatively encouraging.

Authors: Grant Cole, Vitaliy Walker, Daniel Adams, Nathan Twitchell, Melissa Lee, Jennifer Mortensen, Brittney Stockholm, Tatiana LeRoy. Mentors: Claudia Jorgensen. Insitution: Utah Valley University. When exposed to stressful situations, the human nervous system reacts with the fight or flight response activation. Physiological indicators of the activation of the fight or flight response include a change in body temperature, increased heart rate, blood pressure, and sweating (Sriram et.al, 2012). There is mounting evidence implicating stress as physiologically and psychologically harmful. For example, recent research has found a correlation between chronic exposure to stress and the development of mental illnesses such as anxiety and depression. Furthermore, chronic stress has the potential to change the anatomy of the brain along the hypothalamic pituitary adrenal axis (HPA), which is an important part of the neuroendocrine system, plays a role in the release of stress hormones, and helps regulate moods, emotions, and sexual behaviorisms (Ramirez, et.al., 2013). As college can be quite stressful, developing healthy coping mechanisms to deal with stress might positively influence the students’ academic performance and it might improve and maintain their psychological and physiological well-being (Skowronek, 2014). Various research studies have been conducted to reveal the therapeutic qualities of music. Music can effectively relieve nervousness, promote mental health, and positively affect students' psychological state (Chi, 2020). Current research on stress-related outcomes shows that music interventions play a significant role in stress reduction on physiological levels (de Witte et. al, 2020).  Preliminary results indicated that physiological responses (including heart rate, electrodermal skin response, and body temperature) to a mathematical calculation task were not impacted by the type of background noise (control, calm music, or upbeat music). Therefore, the current study is while being exposed to various music genre types (Kirschbaum et al., 1993). We hypothesize that calmer music might reduce stress response, while faster and more upbeat music might increase the stress response. The study will employ a between-subject measures design. After obtaining the baseline physiological measures when no music is played, the participant will either be exposed to two segments of relaxation/mediation music or two segments of fast-beat music. While exposed to different music conditions, the physiological response will be used to compare the impact of music genre (calm meditation music versus techno music) as well as practice (music segment 1 versus music segment 2) on the human stress response. Findings on the effects of music manipulation on the human nervous system can be used as additional tools for the management of stress.

Authors: Minh Nguyen, Eugene Leung. Mentors: Ming Yu. Insitution: Utah Valley University. A rapid, non-invasive method of disease detection method is vital for diagnosis or prevention. Many recent studies suggest that biomarker proteins that are associated with certain diseases were often found in urine samples of the patient. Quantum dots (QDs), a type of nanomaterial, have been investigated in previous studies, revealing their fast response in fluorescent properties when interacting with biomarker proteins. In this study, we examined the impact of QDs’ fluorescent properties in complex solutions by utilizing various concentrations of artificial urine samples. The results of this study provide insight into the possibility of using QDs as an alternative method of disease detection.

Authors: Vince Rossi, Spencer Brown. Mentors: Vince Rossi. Insitution: Utah Valley University. Current systems of generating 3D fluorescent images of samples require scanning of a sample or multiple exposures to acquire a series of images. Collecting a 3D fluorescent image in these fashions is timely, ranging from minutes to tens of minutes. This is not ideal for imaging live, dynamic samples that change as time progresses. The use of holographic techniques allows for the collection of a 3D image on the scale of milliseconds, therefore negating motion artifacts. Combining the speed of digital holography with fluorescent imaging will allow for identification of labeled biological components within a sample as well as the ability to view the sample in a 3D space thus offering greater detail and information on the sample. This process is called Holographically Informed Fluorescence Imaging (HIFI). Simulations have been run using MatLab in which a sample is imaged using Phase Shifting Digital Holography. The 3D reconstruction of the image is propagated using Huygens Convolution Method, and then all of the pixels of the image are brought into focus numerically. This image is then overlayed with the fluorescence image obtained. Each pixel of the fluorescent image is propagated to the depth of focus for each pixel within the 3D holographic image and a 3D fluorescent image is obtained. Simulations of this process have proved successful. A proof of principle experiment is currently underway as to prove the effectiveness of this fluorescent imaging method.

Authors: Sadie Jensen, Nellie Bowman, Nick Jeffery, Heather Wilson Ashworth. Mentors: Heather Ashworth. Insitution: Utah Valley University. The STEM career field has been found to be particularly unwelcoming to women and minorities resulting in attrition of these demographics in STEM degrees and careers.2 To address this problem, the National Science Foundation awarded Utah Valley University a grant to fund undergraduate research opportunities with faculty. This preliminary research project explores the effect of mentored undergraduate research opportunities on student retention and success in the UVU Biology Department. Each year for the last 4 years, 10 students were accepted each year into a research cohort. The following factors are known indicators of student success and retention in the STEM fields: self-efficacy1, effort control4, and teacher immediacy3. These factors were qualitatively measured with reflection questions. Students were surveyed at the end of each academic semester. There are two completed cohorts and data is still being collected on the remaining two cohorts. A coding scheme was developed, and reflection questions were coded to consensus. The Pearson's coefficient was greater than 0.76. The preliminary coding results show themes of positive or very positive effects of the research experience on self-efficacy, effort control, and teacher immediacy. The greatest gains were observed in self-efficacy and teacher immediacy. This preliminary data supports the role of undergraduate research in retaining women and minorities in STEM majors. Sources: Kalender, Z. Y., Marshman, E., Schunn, C. D., Nokes-Malach, T. J., & Singh, C. (2020). Damage caused by women’s lower self-efficacy on physics learning. Physical Review Physics Education Research, 16(1). https://doi.org/10.1103/physrevphyseducres.16.010118 White, J. L., & Massiha, G. H. (2016). The retention of women in Science, Technology, engineering, and Mathematics: A framework for persistence. International Journal of Evaluation and Research in Education (IJERE), 5(1), 1. https://doi.org/10.11591/ijere.v5i1.4515 Micari, M., & Pazos, P. (2012). Connecting to the professor: Impact of the student–faculty relationship in a highly challenging course. College Teaching, 60(2), 41-47. Pintrich, P. R., & De Groot, E. V. (1990). Motivational and self-regulated learning components of classroom academic performance. Journal of educational psychology, 82(1), 33.

Authors: Adam Smith, adam@tallsmith.com. Mentors: Kal Munis. Insitution: Utah Valley University. The social desirability bias is a well-documented and well-studied phenomenon in the social sciences, but it has only been in relatively recent literature that experimentalists have begun to study the effects treatments have on the manner in which the social desirability bias is reflected in social behavior. The aim of this study is to test the effects of perceived privacy influences the way people answer this question: "What is the aim of politics?" This study seeks to replicate findings in other research that has delved into the relationship between perceived privacy and social desirability. Another significant area of interest is how neurodivergent identity, especially autism, fits into the relationship. With this study, we can better understand how social desirability affects political discourse as well as provide insights into the neurodivergent mind and how it relates to politics.

Authors: Bryan Jay Frandsen. Mentors: Joseph McMurray. Insitution: Brigham Young University. This paper expands on a citizen-candidate model of electoral competition under both plurality rule and ranked choice voting. The paper finds that ranked choice voting nominally avoids Duverger’s Law by accumulating many identical candidates but yields fewer viable equilibrium policy positions than plurality rule. Additionally, ranked choice voting favors moderate candidates and policies, increasing the probability of their implementation compared to plurality rule. This moderate bias leads to lower polarization in equilibrium than is possible under plurality rule.

Authors: Ethan Reese. Mentors: Russ Bailey. Insitution: Utah Valley University. Many individuals struggle with oppressively high standards for their character, behavior and environment, know commonly as perfectionism. Perfectionism also carries significant attention in the fields of psychology, at least partially because of its association with higher rates of OCD, depression and anxiety, but also higher performance and goal-achieving (Lunn et al., 2023). The presentation will be synthesized from available literature, and focus on how perfectionism can be positive or negative, and how to effectively adopt beneficial perfectionism. Special emphasis will be placed on the role of perfectionism in academic and workplace settings.There are moderating factors that distinguish adaptive perfectionism from maladaptive perfectionism and various sources of perfectionism, including self-prescribed, socially-prescribed, and other-oriented perfectionism (Benson, 2003). There are diverse explanations of the many ways individuals strive for perfection, or how they impose expectations of it on others, and many of those will be elaborated on. Therapists have both the ability and the professional responsibility to assist in those efforts. The therapeutic modalities specifically suited for working with perfectionism and the consequences of complex relationships between it and certain mental illnesses will be presented for analysis. This discussion will benefit individuals inside and outside of mental health professions alike through exploreing the process of turning perfectionism adaptive, as well as provide direction for future research in on the topic.References:Lunn, J., Greene, D., Callaghan, T., & Egan, S. J. (2023). Associations between perfectionism and symptoms of anxiety, obsessive-compulsive disorder and depression in young people: A meta-analysis. Cognitive Behaviour Therapy, 52(5), 460–487. https://doi.org/10.1080/16506073.2023.2211736Benson, E. S. (2003, November 1). The many faces of perfectionism. Monitor on Psychology, 34(10). https://www.apa.org/monitor/nov03/manyfaces

Authors: Libby Brooks. Mentors: Natasha Pavlova. Insitution: University of Utah. About 90% of cancer deaths are from the development of secondary tumor growths from a process called metastasis. As cancer cells divide uncontrollably nutrients from nearby tissue – specifically the amino acid glutamine – are depleted. Glutamine is one of the most abundant amino acids in the bloodstream, and most cells cannot live without it. Tumors have poor vasculature which further contributes to the depletion of nutrients. Even in nutrient-depleted environments, tumors have found ways to grow.Tumors are comprised of cancer cells as well as non-cancerous stromal cells. Studies have shown stromal cells can synthesize glutamine which they release into the tumor microenvironment feeding cancer cells and allowing them to grow. However, the signaling pathway used between the cancer and stromal cells in this relationship remains unclear.To study the signaling pathway by which stromal cells are synthesizing glutamine I treated cells with a common anti-inflammatory drug, dexamethasone. Mice receiving dexamethasone over a long period of time had increased lung metastases when injected with cancer cells. Dexamethasone binds to the glucocorticoid receptor (GR) which activates the expression of glutamine synthetase (GLUL) enzyme mRNA. The expression of GLUL causes the cell to synthesize glutamine. However, the role of GR, in regulating GLUL expression in tumors, remains insufficiently studied.The experiments done for this project show that dexamethasone induces GLUL expression in stromal cells such that they start releasing glutamine. This may promote growth of cancer cells even when there is a deficit of nutrients around. It is hypothesized that due to this property, dexamethasone increases risk of metastasis. This project will aid in the development of cancer therapeutics to treat metastatic cancers.

Authors: Ryan Kunkler, Marcus Lawrence, Anna Edler, Casey Webb, Jacob Manning. Mentors: Marcus Lawrence. Insitution: Southern Utah University. Climbing is now an Olympic sport and thus the demand for understanding performance predictors to train with evidence has grown enormously. Previous climbing research has highlighted that finger strength and shoulder strength are important predictors of performance between lower level and higher level climbers. However, no study has examined the rate of force development in the upper body, and lower body strength also has not be assessed in climbing literature. PURPOSE: To determine if relationships exist between dominant and non-dominant finger RFD compared to dominant and non-dominant finger and shoulder strength as well as lower body strength. METHODS: Twenty subjects (n=8 female and n=12 male; age: 24.7±7.5 yrs; height: 177.6±7.8 cm; mass: 76.0±14.9 kg; IRCRA Sport Grade: 14.1±6.7; n=11 beginner/intermediate, n=9 advanced) completed this study. During a single session, following a standardized 3-5 min. warm-up all participants dominant and non-dominant finger strength and RFD (using a Tindeq dynamometer load cell attached via static rope to a 20mm edge) as well as shoulder strength (using the same Tindeq load cell with a static rope and olympic ring), and lower-body compound strength (isometric mid-thigh pull using G-strength dynamometer load cell attached to a straight bar with a static rope) were assessed. Three trials were done on each measurement with 1 min. between trials and 3-5 min. between tests. Pearson correlational analyses were done to determine correlation coefficients (r), with significance set at p<0.05. RESULTS: Both dominant and non-dominant RFD resulted in significant (p<0.05) large to very large positive relationships with dominant finger strength (r = 0.897 and 0.721, respectively), non-dominant finger strength (r = 0.913 and 0.757), dominant shoulder strength (r = 0.670 and 0.709), on-dominant shoulder strength (r = 0.724 and 0.744), and lower body compound strength (r = 0.645 and 0.653). CONCLUSION: Dominant and non-dominant RFD is positively related to upper and lower body strength in recreational to advanced climbers. Therefore, training finger RFD and lower body strength should be consider as important as developing finger and shoulder strength in recreational to advanced climbers.

Authors: Daniel Barrera. Mentors: Anna Beaudin. Insitution: University of Utah. Tissue-resident macrophages play critical roles in tissue homeostasis and immunity, and many of them have a distinct fetal origin and developmental trajectory as compared to their adult bone marrow-derived counterparts. However, the specific mechanisms underlying their developmental signaling pathways have not been as thoroughly examined as in the adult. Our lab recently demonstrated that fetal-derived macrophage development is regulated by expression of the lymphoid-associated interleukin-7 receptor (IL-7R) in mice, but the fetal source of the cognate cytokine ligand, interleukin-7 (IL-7), has yet to be determined. This project investigated fetal macrophage cells as a potential source of IL-7 production, with the aim of providing further insight into these signaling pathways during prenatal development. A transgenic mouse model was used that expresses IL-7 attached to green fluorescent protein (GFP) in order to measure GFP expression as a proxy for IL-7 expression in developing tissues. Embryonic tissues were extracted from mouse fetuses at 17.5 days post-conception and cells were isolated and stained with antibodies to identify blood, endothelial, and stromal cells as putative sources of IL-7 production. Samples were also intracellularly stained for GFP in order to quantify the IL-7 production across different cell types within different tissues in the embryo. The resulting data preliminarily identifies fetal macrophages as the primary producers of IL-7 across common tissues in the developing embryo. A better understanding of the developmental signaling pathways that regulate fetal immune development can expand comprehension of the origins of early immune dysfunction and help mitigate disease susceptibility from early life.

Authors: Amber Brooks. Mentors: Michelle Schober. Insitution: University of Utah. Traumatic brain injury (TBI) can cause death or result in long-term disabilities. TBIs are the leading cause of death and disability due to trauma and the leading cause of acquired neurological disability in children. At present, medical treatment using supportive and specific therapies to optimize recovery are lacking. Docosahexaenoic acid (DHA), a component of fish oil and a natural constituent of brain cell membranes, is a potential candidate therapy to improve neurologic recovery after severe TBI. The objective of our project is to test the hypothesis that DHA improves cognitive outcome and brain imaging in a male rat model of pediatric TBI, controlled cortical impact (CCI) in male rat pups. Seventeen-day-old male rat pups received CCI or a minimally invasive (SHAM) surgery. Half of the CCI and SHAM rats were fed a DHA diet for 60 days before experiments began. Learning was tested using a Morris Water Maze (MWM) experiment and memory was tested using a Novel Object Recognition (NOR) experiment. Brain injury assessment was accomplished using T2 and DTI magnetic resonance imaging (MRI). The T2 MRI scans were used to measure the volume of the hippocampus, the center for learning and memory, and to lesion volume, to assess loss of hemisphere volume. The DTI MRI scans were used to observe the injury to the white matter of the brain. Based on preliminary findings, we anticipate that the results of the study will not support our hypothesis and that DHA will have no beneficial effects on improving cognitive outcomes and brain imaging.

Authors: Addison Hedges, Kevin Sattler. Mentors: Moriel Zelikowsky. Insitution: University of Utah. Trauma has been reported to affect over 70% of all adults worldwide. This includes, but is not limited to, physical and sexual violence, injury, threat of death, and natural disasters. Experiencing one or more traumatic events can lead to post-traumatic stress disorder, depression, and anxiety. These symptoms and disorders can negatively affect interpersonal relationships, confidence, aggression, and other aspects of daily behavior. Exactly how these traumatic events are encoded in the brain is poorly understood. Extensive research has been done on the hippocampus with regard to its central role in learning and memory, but only recently has research been done to uncover its role in emotional responses. This research has found distinct functional differences between the dorsal and ventral subregions of the hippocampus. Recent studies suggest that the ventral hippocampus (VH) plays an important role in emotional response. The VH projects to other emotional processing areas of the brain that modulate fear, aggression, and social processing. This project aims to test the hypothesis that distinct populations of neurons in the VH encode individual behavioral effects of trauma as well as discover the extent of overlap between neuronal cells activated by trauma-induced aggression (TIA) vs. trauma-altered sociability (TAS). To test this hypothesis, both male and female mice were injected with an hM4D inhibitory DREADD virus to chemogenetically silence VH. Later the mice underwent either no trauma or a foot shock trauma consisting of 10 1mA foot-shocks randomly applied over 60 minutes. They were then tested for aggression and social response using two distinct behavioral assays known as Resident Intruder and 3-Chamber. Half the mice received DCZ, a ligand used to activate DREADDs, while the other half received a control vehicle prior to each behavioral assay. Our findings show that silencing of the VH led to attenuated TIA and TAS when compared to control groups. Next, using retrograde viral tracing, activity-dependent neuronal tagging, and immunohistochemistry, we examined the existence and extent of overlapping neuron populations in the VH. These findings suggest the VH plays an important role in encoding and responding to trauma. They also highlight the importance of continued research into how behavioral changes are manifested in the brain and how these experiences are studied.

Authors: Allison Perkins, Aubrey Hawks, Talia Karasov. Mentors: Talia Karasov. Insitution: University of Utah. Over the past two decades, studies have documented a 20% decline in Quaking Aspen (Populus tremuloides) populations in western North America (Worrall et al., 2015; Stanke et al., 2021). This phenomenon has been fittingly characterized Sudden Aspen Decline (SAD), and is an increasingly pressing issue as the role of aspen as an ecologically irreplaceable keystone species impacts the health of the surrounding forests (Singer et al., 2019). SAD has been attributed to the interplay of climate change-driven drought and other biotic and abiotic factors that are less well characterized (Anderegg et al., 2013a). One potential contributor to SAD is biotic pests and pathogens (Marchetti et al., 2011; Anderegg et al., 2013a; Worrall et al., 2015). My study system includes both natural populations of aspen representing a precipitation gradient and a controlled garden experiment. The field experiments span five distinct sites across Utah & Colorado, selected and montintered by the Anderegg lab of the University of Utah. The experimental garden contains approximately 300 tree saplings subjected to various levels of drought stress, managed by the Anderegg lab on the University of Utah campus. Through the integration of both controlled and natural experiments, my research aims to comprehensively evaluate the impact of drought exposure on pathogen abundance and chemical defenses in aspen trees.Over the past two decades, studies have documented a 20% decline in Quaking Aspen (Populus tremuloides) populations in western North America (Worrall et al., 2015; Stanke et al., 2021). This phenomenon has been fittingly characterized Sudden Aspen Decline (SAD), and is an increasingly pressing issue as the role of aspen as an ecologically irreplaceable keystone species impacts the health of the surrounding forests (Singer et al., 2019). SAD has been attributed to the interplay of climate change-driven drought and other biotic and abiotic factors that are less well characterized (Anderegg et al., 2013a). One potential contributor to SAD is biotic pests and pathogens (Marchetti et al., 2011; Anderegg et al., 2013a; Worrall et al., 2015). Recent investigations have indicated a link between SAD and specific microbial diseases, suggesting that the increasing frequency and severity of droughts due to climate change might make aspen more vulnerable to certain pathogens, even though many of the most common pathogens of aspen in general require more abundant water (Aung et al., 2018). For example, the foliar Melamspora fungal pathogens require abundant water and are not frequently observed in drought stressed trees.On the other hand, Cytospora, which causes a devastating canker disease in aspen trunks, occurs at higher frequency in plots of trees suffering damage from drought (Guyon, 1996). Lin et al. (2023) shows changes to phyllospheric microbiome in aspen during drought, but far less is known about the leaves specifically. Could drought lead to an altered microbiome in aspen leaves? If different or possibly opportunistic pathogens are better able to colonize the leaf tissue under drought stress, this may be the case.It’s known that plants with a reduced diversity of microbiomes are more susceptible to pathogens (Zheng et al., 2020), but the there is little understanding how drought may reduce microbial diversity in aspen. Aspen have two main groups of chemical defenses (SPGs and CTs) that occur in relatively high levels in the leaf (Lindroth et al., 2023). It is well established that these defend against insect herbivores and that they come with a trade-off for plant growth (Marchetti et al, 2011). There is some observational evidence that these secondary compounds have an effect on pathogens (Jacoby et al., 2021), but there are few controlled studies on this idea. Additionally, Metlen et al. 2009 describes how trees in North America produce higher rates of these metabolites under wetter conditions, attributed to possible UV protection. However, the impact of additional environmental changes have of on the abundance of these compounds is understudied. Aspen are an ideal system to study forest disease and drought because the genus’ (Populus) genomes are easily sequenced and relatively tractable, aspen generally exhibit rapid vegetative growth, their defensive chemistry is relatively well known, and most importantly, they grow in cloned groves that reduce the genetic variation in experiments (Lindroth et al., 2023, Luquez et al., 2007).

Authors: Braxton Bird. Mentors: Sarah Franklin. Insitution: University of Utah. Heart disease ends the lives of nearly 700,000 people each year and has been the leading cause of death in the United States since 1950. Around this time researchers discovered that some modifications involving our genetic code could be altered to affect gene expression but leaves the DNA intact, which was later termed epigenetics. Today we’ve discovered that these epigenetic modifications, including post translational modifications (PTMS), regulate genes linked to cardiovascular disease. We recently examined the histone lysine methyltransferase SETD7, which is most prominently known for its ability to methylate histone H3K4. SETD7’s expression is upregulated in multiple types of heart disease in both humans and mice and is essential for cardiomyocyte differentiation in embryonic development. In addition to its ability to methylated H3K4, SETD7 has been shown to methylate 8 other histone residues. To further characterize the histone residues methylated by SETD7, we carried out an unbiased analysis of lysine residues methylated by SETD7 using an in vitro methyltransferase assay coupled with tandem mass spectrometry. We hypothesized that SETD7 may modify additional sites than those that have previously been identified. Our analysis determined that SETD7 monomethylates two novel residues on histone H3: lysine 36 (K36) and lysine 122 (K122). These sites of modification were also confirmed by western blotting for site specific antibodies to these methylation marks. Although our understanding of both these residues is limited, we do know that K36 methylation is linked to DNA replication and genomic stability while K122 methylation is downregulated in drug-resistant MCF-7/ADR cancer cells. These two novel methylation sites suggest that this lysine methyltransferase plays a more complex role in regulating epigenetic modifications and gene expression than previously recognized. Although the identification of this new enzymatic activity for SETD7 is important for understanding the dynamic function of methyltransferases, additional studies will be necessary to fully elucidate the role of SETD7 in cardiac physiology and gene regulation.

Authors: America Cox, Kendra Autumn , Bryn Dentinger. Mentors: Bryn Dentinger. Insitution: University of Utah. Neotropical ants of the Attini tribe evolved the innate ability to farm fungi. Agaricomycetidae contains two clades that are cultivated by ants: the lepiotaceous and pterulaceous cultivars. However, there are free-living relatives phylogenetically distributed throughout each cultivar clade. Comparison between the free-living relatives and attine system cultivars may identify the evolutionary differences caused by, or initially enabling, agricultural symbiosis. Our research compares free-living relatives and cultivars through genomic and metabolic analysis. Attines undertake significant cultivar maintenance through the regulation of pathogenic contamination of their fungal “gardens” as well as the provision of specific growth substrates, including insect frass. Noting the apparently specialized substrates cultivars receive from the ants, we conducted a pilot test for a growth media preference between a lepiotaceous cultivar, a pterulaceous cultivar, and a free-living pterulaceous relative. We placed the fungi on regular PDY media and PDY media infused with caterpillar frass, and the cultivars either changed structure or had improved growth on the frass-infused media. Following the pilot test, we will run metabolic assays on the cultivars and free-living relatives on different media types. This may indicate a media preference which gives further insight to the attine-fungal symbiotic relationship opposed to the fungal free-living relatives revealing pieces of the fungi’s evolutionary history. We performed DNA extraction, PCR testing, Sanger sequencing of the ITS region, and then whole genome sequencing on the cultivars and their free-living relatives. Sanger sequencing allowed us to build phylogenetic trees to examine the relationship between the free-living fungi and cultivars. The whole genome sequencing allowed us to use antiSMASH software to generate predicted secondary metabolite clusters in a fungi species that “escaped” cultivation, a cultivar, and a free-living relative. This preliminary data suggests a diversification of fungal secondary metabolites occurs after attine domestication. By looking at fungal metabolic and genomic data, we hope to gain insight into the fungi’s evolutionary history and agricultural symbiosis.