Jorgen Madsen, Utah State University
Health
The gold standard in purification of influenza virus is by means of ultracentrifugation. Although effective, this process is very expensive and thus impractical for developing countries. We hypothesize that column chromatography can be a cost efficient alternative that is as effective as ultracentrifugation. If correct, this method of purification could revolutionize vaccine production in third world countries. We tested the purification ability of column chromatography by comparing two different chromatography resins. The Capto Q resin separates proteins on the basis of protein charge. The Capto 700 resin separates proteins on the basis of both size and charge. Samples following chromatography were collected, and evaluated for virus specific proteins as well as total protein content. After chromatography selected samples were evaluated by electrophoresis to determine protein separation. Although it is evident that some protein separation occurred, the results are inconclusive and suggest more testing.