Author(s): Jackson Thomas, Maxwell Richtsteig
Mentor(s): Jessica Pullan
Institution SUU
Hyperemesis Gravidarum (HG) is a severe form of nausea and vomiting experienced during pregnancy, affecting up to 2% of pregnancies. This condition’s symptoms are debilitating and can lead to hospitalization and, in extreme cases, miscarrige and death. HG has led to an estimated annual healthcare costs exceeding $1.7 billion in the U.S with no currently effective treatments. Recent research suggests the association between HG and the GDF15 protein, as specific mutations and genetic patterns within the GDF15 gene appear more frequently in HG patients compared to women without HG. Our study seeks to further investigate this potential genetic link by analyzing local demographics using polymerase chain reaction (PCR) and DNA sequencing techniques. To enhance our DNA purification process, we conducted testing on various elution buffer conditions to optimize yield and purity, ensuring high-quality DNA for downstream analysis. Additionally, we carefully selected and tested PCR primers specifically designed to amplify regions of interest within the GDF15 gene, particularly those associated with known HG-linked mutations. Our findings so far have narrowed the focus to the C211G mutation, as evidence from our research suggests that other mutations may not be associated with HG as previously thought. By understanding the connection between the C211G mutation and HG, we hope to support the development for more precise diagnostics and targeted treatments, potentially alleviating the burden of this condition for pregnant women. This study’s insights contribute to the growing body of HG research and may offer new avenues for effective management strategies in the future.