Author(s): Isaac Christensen
Mentor(s): Kiara Whitley
Institution UVU
This study aims to quantify the effects of Lactobacillus rhamnosus GG on the composition of the human gut microbiome. Historically, the role of bacteria in human health has been misunderstood, often viewed solely as pathogenic. However, recent research has illuminated the importance of beneficial bacteria, known as probiotics, in maintaining health. Probiotics play a vital role in various physiological processes, and their influence on the gut microbiome is gaining scientific attention. This study focuses on L. rhamnosus GG, a widely discussed probiotic, and its potential to alter the gut microbiome's composition and diversity. To investigate this, we will conduct a comprehensive bacterial DNA analysis of fecal samples from a single participant before and after a 21-day period of probiotic supplementation with L. rhamnosus GG. Samples will be collected twice: once prior to supplementation and again after the probiotic has been taken. The participant will be instructed to maintain their usual diet and lifestyle to minimize confounding variables. We will perform a DNA extraction to eliminate impurities and use PCR to amplify the 16S rRNA gene, allowing us to identify bacterial presence. Using the MinION nanopore sequencer and Epi2Me software, we will analyze the bacterial composition and quantify the changes in proportions of various genera. By comparing pre- and post-supplementation samples, this research aims to show the effects of L. rhamnosus GG on the gut microbiome, contributing to the growing body of knowledge surrounding probiotics and their health implications. Understanding these changes will provide valuable insights into the potential applications of probiotics in healthcare, paving the way for further research into their role in promoting gut health and overall well-being.