Funk, Stephen; Wood, Jacob; Catmull, Ashley; Martin, Brett (Brigham Young University)
Faculty Advisor: McCleary, Bill (Brigham Young University College of Life Sciences, Microbiology & Molecular Biology)
The survival of the model microorganism E. coli depends largely on its ability to regulate the concentration of nutrients in its cell. This regulation often relies on complex systems of both cooperative and competitive enzymes. One of these enzymes, encoded by the YjbB gene, was known to play some role in phosphate regulation. However, its exact function had not yet been characterized. We attempted to deduce the function of the YjbB-encoded protein in the context of two other phosphate regulatory systems: the PitA/B phosphate transport system and the PpK/X polyphosphate storage system. Using comparative growth curves in both phosphate-rich and phosphate-deprived media, we found that the YjbB-encoded protein protects against phosphate poisoning in the absence of phosphate exporters, suggesting that the protein in question functions primarily as a phosphate exporter as well.