Marston, Clarissa; Garrett, Patrick I.; Barraza, Allyson; Allen, Nicole ; Hillhouse, Todd M.; Honeycutt, Sarah (Weber State University)
Faculty Advisor: Hillhouse, Todd (Weber State University, Psychology)
Over the last decade, electronic-cigarettes use has substantial increased. E-cigarette usage in high school aged Americans has increased 78% from the year 2017 to 2018, with a total of 20.8% of all teenagers reporting regular usage as of 2018. The adolescent population is particularly attracted to the nicotine-free e-liquids that are currently on the market. While current research has focused on examining the behavioural implications of nicotine vapour inhalation but little has been devoted to evaluating the effects of nicotine-free vapour administration. The present sought to examine how nicotine-free vapour inhalation would impact locomotor behaviour. To accomplish this, mice were placed in a vape apparatus and administered the selected puff (1, 3, 6, or 10 seconds) of unflavoured 50/50 oil blend of propylene glycol and vegetable glycerine of vaporised e-liquid every 2 minutes during a 10 or 30-minute session. Following administration, mice were placed in open field arenas to measure locomotor activity as well as record time spent in thigmotaxis, a known measure of anxiety. Vapour administration produced significant sex differences on locomotor activity and thigmotaxis time. Specifically, all puff durations significantly decreased locomotor activity and increased thigmotaxis time in female mice. For male mice, the 6 and 10 sec puff decrease locomotor activity and increase thigmotaxis time. Results of this study indicated that there are dissociable behavioural effects following administration of nicotine-free vapour inhalation in mice that are dependent on sex, puff duration, and administration session length.