Brad Bundy; Nelson, Andrew; Welton, Meagan (Brigham Young University)
Faculty Advisor: Bundy, Brad (Brigham Young University, Chemical Engineering)
Cell-free protein synthesis (CFPS) is the process of making proteins without the limiting environment of a cell. The CFPS system allows us to modify and engineer proteins in ways that have not been done before. This technology has the potential to significantly impact the fields of protein therapeutics, unnatural amino acid insertion, and biosensors. Our research looks into optimizing the process of CFPS. Specifically, we want to understand what the impact of adding bubbles to a cell-free reaction is on CFPS yields. We want to know if adding bubbles to the CFPS mixture prior to incubation will increase the oxygenation of the reaction and affect protein production rates. This knowledge will help us optimize the CFPS process for future applications.