2013 Abstracts
Whatever Happened to Salt Lake City’s Chinatown?
Licia Kim, Utah Valley University Academic Affairs Utah is a state that was largely settled by immigrants, and among those immigrants were hundreds of Chinese people. For over 70 years, Salt Lake City was home to one of the most prominent Chinatowns in the Intermountain West. Today, Utah is home to over 10,000 Chinese people, but there is no Chinatown in Utah. If the average Utahan is asked “Whatever happened to Salt Lake’s Chinatown?” the answer will invariably be a variation of “Salt Lake had/has a Chinatown?” Yes, Salt Lake City had a Chinatown and this research project answers the question of what happened to it. By exploring existing scholarly works, oral interviews and newspaper articles from the years that Plum Alley, Salt Lake City’s Chinatown, existed, I examined the creation, heyday, decline and eventual demise of Utah’s largest Chinatown. According to my research, the Plum Alley Chinatown disappeared because of a combination of cultural/religious differences, economic pressures, racial issues, and political/legal restrictions. As the significance of China, both to Utah and the United States as a whole, increases, an opportunity is created to increase public awareness of the Chinese experience in Utah’s history. Once this history is known, Plum Alley’s location, in a familiar, near-by location, will provide students in Chinese immersion classes (and their parents) with an opportunity to form a more immediate connection with these distant people. The goal of this research is therefore twofold: 1) to create a research article suitable for publication in a scholarly journal, and 2) to create a PowerPoint presentation and/or traveling historical exhibit on Plum Alley suitable for display at schools, public libraries and other community centers (such as the new South Salt Lake Chinatown shopping center). Simple entrance and exit surveys will allow me to track the effectiveness of the presentation/exhibit to increase visitor awareness of this little known chapter in Utah history.
The Effectiveness of Relationship Education in a College Course
Andy Thompson, Utah Valley University Academic Affairs The effectiveness of relationship education, especially among predominately young college students, has been a topic of great interest. Given the diversity of family outcomes, more young individuals seek out help for their relationships. Past studies involving relationship education programs have showed that such programs are beneficial (e.g., Halford, et al., 2003). Improvement in couple communication skills was one effect of couples participating in these programs, and even increased relationship satisfaction in some instances (Halford, et al., 2003).
Detecting the Genetic Signatures of Breast Cancer with High-Frequency Ultrasound
Janeese Stiles, Utah Valley University Academic Affairs Previous studies have shown that high-frequency (HF) ultrasound is sensitive to cell properties such as stiffness and adhesion factors which are a function of protein expression. The goal of this project is to see if HF ultrasound is sensitive enough to detect and differentiate between the five molecular subtypes of breast cancer which are based on protein expression. Since genetic changes precede histological changes in the development of breast cancer, the ability to detect genetic changes (i.e., molecular subtypes) in breast tissue in real time and at the microscopic level will allow surgeons to remove all of the malignant and premalignant tissue during lumpectomies. HF ultrasound personalizes the treatment plan and will be used as a diagnostic technique for precise, image-guided breast cancer surgery. Four breast cancer cell lines with different molecular subtypes and a non-malignant breast cell line will be grown as monolayer cultures. At monolayer confluence, cell and nuclei morphologies of the cell cultures will be determined by phase-contrast microscopy. After microscopy, the monolayers will be ultrasonically tested using a HF ultrasonic test system with a single-element (50 MHz, 6.35-mm) ultrasonic immersion transducer. The resulting ultrasonic waveforms will be analyzed using computational models that simulate the ultrasonic scattering from cells and nuclei as a function of morphology, internal properties, and external properties. The protein expressions associated with the different subtypes will be researched to determine what effects each subtype will have on cell and tissue properties. This method will add a new dimension to pathology and permit more efficient surgical treatment of breast cancer.
Methods for Identifying Aerosols by Light Scattering Techniques
Laurel Thompson, Utah Valley University Academic Affairs Soldiers and non-combatants are at risk of exposure to dangerous aerosols (airborne particles or droplets) in the form of biological agents such as bacteria, toxins, or viruses. The current method for assessing health risk in the field is a moist swath which turns dark upon contact with a biological aerosol. Optical methods are more sensitive to the physical properties of aerosols, and many systems have been developed for optically measuring particle properties. However, they are generally limited to bio-aerosol detection at a single point in space where the system directly samples an aerosol from within the aerosol cloud. The desired solution is a system that can employ remote sensing to measure aerosol properties from a distance. Standoff detection methods allow a much larger area to be measured at once, providing a more general or big-picture view of the aerosols in a given area. There are several ways that standoff optical scattering data can be analyzed for determining aerosol properties. Light scattered by aerosols of known size and composition can be modeled exceptionally well with Mie scattering theory, but the reverse problem determining aerosol properties from the light signals using inverse Mie theory is difficult to solve because a unique set of aerosol properties must be found to correspond to the optical spectra. This is challenging since different combinations of aerosol properties can result in similar spectra. The size distribution, however, has a large effect on the optical signal and may therefore be used to differentiate aerosols. Specifically, biological aerosols have a more narrow size distribution than mineral-based dusts due to genetic limitations. Their refractive indices will also contribute to distinct optical spectra. The hypothesis for this project was that these factors would be sufficient to classify aerosols for risk assessment. Three analysis methods were used to test this hypothesis: Mie inversion with matrix solutions, empirical curve fitting with polynomial functions, and principal component analysis (PCA). Particles suspended in methanol were used as the model aerosol system. A range of particle sizes and compositions were illuminated by a balanced deuterium/halogen light source and spectral measurements from 200-1100 nm were taken. Optical data over a 200-1300 nm range were also collected from a variety of bio-aerosols using an open path remote sensing system at a 30-meter standoff distance.
Emerging Trends in Health Promotion: Are the needs of Health Education Specialists being met?
Chelsea Newsome, Utah Valley University Academic Affairs In the emerging profession of health education there has been a lack of attention on the needs of health education specialists. Recently at Utah Valley University, Dr. Mary V. Brown, and undergraduate students in the Public and Community Health Education department, conducted research focusing on if the needs of health education specialists were being met. Qualitative data collection methods were used to help in needs assessment, planning, goal setting, and quality improvement for both professional organizations and academic institutions. Student Researchers held five focus groups throughout the state of Utah to gather insights from health educators on what they believe are emerging trends, most useful coursework in the academic setting, and professional development needs. The questions used in the focus groups were developed from the assistance of two state professional organizations. The students involved were trained to be moderators and note takers by using guidelines from Krueger and Casey (2000). The data was analyzed using the long table approach outlined by Krueger and Casey (2000). The results showed that the health educators perceived the top emerging trends were social marketing, followed by social media, and a greater focus on primary prevention. As researchers we found the most helpful information for academic institutions would be what the health educators felt were lacking in their educational experiences. The health educators wished that grant writing, computer programs/technical skills/ designing brochures, real life experience/opportunities to apply their knowledge in community settings/practical application classes had been offered in their program of study. With regard to professional development, we found the majority of health educators were supported from upper management to receive continuing education depending on funds. The classes they wanted more of at these continuing education conferences are knowledge in politics, technology, and developing partnerships in the community. This information will be a benefit to both the professional organizations and academic institutions in the state of Utah to improve training of professionals and students alike.
Comparative Study of Three Invasive Thistle Species Seed Viability
Johonna Sheldon, Southern Utah University Agriculture Science Scotch Thistle (Onopordum acanthium), Musk Thistle (Carduuas nutans) and Bull Thistle (Cirsium vulgare) are invasive species that produce large quantities of seeds that remain viable for long periods of time. Collections were made of the species from various locations. Five distinct stages of growth were determined. Samples were collected and labeled with the maturity stage of each seed pod recorded. Tetrazolium, cut, and pop tests will be used to help indicate seed viability. I hypothesize that different stages of maturity will have varying degrees of viability as determined by tests. Furthermore I predict later stages will have the most overall viability and the pop test will positively correlate with the tetrazolium and cut tests. The results are in progress.
Use of Anomalies in the Earth’s Total Magnetic Field to Locate Copper, Gold, and Silver Deposits in Fissure Veins, Tintic Mining District, Central Utah
Michael Alexander, Utah Valley University Academic Affairs The Tintic Mining District is located in central Utah on the eastern edge of the Basin and Range Province. This area experienced significant hydrothermal alteration associated with volcanism in the early Cenozoic Era. This hydrothermal alteration was productive of sulfide ore deposition along fissure veins, including ores of copper, gold and silver. Previous aeromagnetic surveys showed that porphyry copper assemblages are associated with mappable anomalies in the Earth’s total magnetic field. The magnetic anomalies were interpreted as resulting from buried intrusive igneous rocks associated with the porphyry copper assemblages. The objective of this research is to map buried fissure veins on property owned by NorthStar Mine using a ground-based survey of anomalies in the Earth’s total magnetic field. This study will be the first geophysical mapping of fissure veins in this area. Previous work by the author and other Utah Valley University students showed that total magnetic field anomalies could be used to map halloysite clay deposits, the copper sulfide deposits associated with buried basaltic dikes, and a wide variety of igneous rock bodies including buried bodies of quartz monzonite, rhyolite and tuff. Because the igneous rocks are considered to be the source of hydrothermal fluids, further mapping of the distribution of igneous rocks could give some insight into the migration of fluids that deposited ore in fissure veins. The ground-based magnetic survey will be carried out using the Geometrics G-856 Proton Precession Magnetometer. Magnetic susceptibilities of outcrops will be measured using the handheld SM-10 Magnetic Susceptibility Meter. Rock samples will also be collected for crushing and more precise measurement of magnetic susceptibility in the lab using the Bartington MS3 Magnetic Susceptibility Meter. Mapped magnetic anomalies will be compared with possible subsurface rock bodies using the IX2D Magnetic Interpretation Software. All necessary equipment is currently owned by the Department of Earth Science. This research is being carried out in cooperation with NorthStar Mine. Results will be reported at the meeting.
Actual Heart Rate vs Participant Predicted Heart Rate in College Sport Activity Classes
Alex Dutson, Utah Valley University Academic Affairs Introduction:
Expertise Development Opportunities for University Honors Students
John Maynes, Utah State University Elementary Education Research on gifted education has shown that expertise development requires learning very basic skills to a high level (Bloom 1986). As a university education is meant to provide students with opportunities to develop expertise in a given field, it is imperative that universities provide learners with opportunities to learn very basic skills to a high level. However, do students receive adequate opportunities to learn the skills necessary to develop expertise? Do some students receive more opportunities than others? This study seeks to answer these questions by surveying honors and non-honors students to determine their perceptions respectively of opportunities to develop expertise in their chosen field of study. By matching each honors student with a non-honors student in the same class, the survey aims to evaluate the difference between the perceptions of honors students and non-honors students in the same class. Scager, et al indicate that honors students are more likely to develop expertise than non-honors students at a university (2011). Thus the hypothesis of this study is honors students receive more opportunities for expertise development than non-honors students.
Riparian Vegetation Change Jordan River, Utah
Jonathan Hilbert, Utah Valley University Academic Affairs The Jordan River in Utah has been highly regulated for many years through intense irrigation, channelization, and managed releases from Utah Lake. As the only outlet of Utah Lake flowing north into the Great Salt Lake, it is important to the riparian ecosystem and the surrounding human population as well. The river has a long history of mixed-uses, but it is emerging as a popular recreation site for the numerous adjacent communities. Human interaction on the individual and commercial scale influences the river through development and urbanization. The physical characteristics of the river and its riparian zone can be monitored to understand how the landscape is changing over time. In this study we focus on land cover changes in the riparian zone of the upper one-third of the river flowing out of Utah Lake to what is called “the point of the mountain”. This is the portion of the river that flows in Utah County. From aerial imagery and field observations, we noted alterations in the vegetation within the riparian zone from channelization, riprap, invasive species, or removal for a variety of reasons. We use GIS and aerial imagery to evaluate the land cover change on the Jordan River in Utah County between 1992-2011. Using Anderson’s classification system of land cover, specifically Urban or Built-Up Land, Agriculture, Vegetation, and Barren Earth, we quantified the changes in vegetation within a 100 meter buffer of the river’s wetted channel. While there are areas where little change is observed, areas of greatest change occurred downstream of the outlet, where a number of new communities have been developed in the last decade. As a subset of Vegetation we mapped changed in invasive species, tamarisk and reed canary grass, along the same section of the river within the riparian zone. Urban planning and invasive species removal along the river needs to be further considered as potential future recreation and restoration efforts are advanced on the river.
A Study on Public and Private Education in Ecuador
Andres Chavez, Brigham Young University Education The purpose of this research is to explore the current status and condition of the public and private education k-12 in Ecuador and analyze the implications that recent policy changes made by the Ecuadorian Ministry of Education hold for the future role of K-12 schools in the country. In order to do this, I first met with the Minister of Education and received information regarding national test scores of schools in six provinces, the provinces being Guayas, Santa Elena, El Oro, Manabí, Azuay, and Pichincha. I then visited four different types of schools in each of those provinces, the types being public, municipal, fiscomisional, and private. In each of these schools, I held an interview with the principal as well as a few selected teachers, and observed the general condition of the school grounds and facilities. I found that the majority of the public schools are overpopulated, while the private schools for the poor are disappearing due to the diminishing number of students who are enrolling in their programs. I propose that the reforms that the Ministry of Education has put in place will be more effective in improving education if the number of students in each public classroom is reduced to a manageable amount. This can be achieved by the government providing subsidies and financial aid for more students to attend private schools.
Comprehensive Mentoring Program
Brandon Dollar, Utah Valley University College Success Studies Student retention and persistence to graduation are two issues that receive significant attention and allocation of financial and human resources in higher education. A recent study (Creighton, Creighton, & Parks, 2010) stated that 26% of new freshmen do not matriculate their sophomore year. Consequently, many institutions within higher education have placed emphasis on the first-year experience (FYE). In response to the issues that lead freshmen to dropout, FYE program outcomes generally include student self-awareness (Krause & Coates, 2008), learning academic skills and strategies (Crisp & Cruz, 2010; Schrader & Brown, 2008), connecting students to campus (Zhao & Kuh, 2004; Bell, 2012) and social support (Tinto, 2009; Wilcox, Winn & Fyvie-Gauld, 2005). However, research shows that most institutions approach FYE programming with a “piecemeal approach” (Krause, Hartly, James, & McInnis, 2005) and do not take a “whole-of-institution” approach (Kift, Nelson, & Clark, 2010) where student retention and persistence are part of the fabric of the entire institution. One of the aspects of FYE programming is peer mentoring. Peer mentoring emerged within the FYE programs to facilitate these program outcomes and ultimately, student retention. In fact, an early literature review (Jacobi, 1991) on mentoring revealed four identifiable outcomes of mentoring, three of which claimed to be applicable to peer mentoring and successful fulfillment of FYE outcomes. Some peer mentoring programs emerge from student affairs (Lopez, Johnson & Black, 2010) while other programs originate from academic affairs (Colvin & Ashman, 2010). Aside from the differing origins of peer mentoring programs, research identifies six types of mentoring programs, five of which claim to be applicable to peer mentoring (Karcher, Kuperminc, Portwood, Sipe, & Taylor, 2006). However, there still appears to be no universal approach to peer mentoring in higher education. Specifically, research has yet to reveal a peer mentoring program that comprehensively synthesizes the great foundational work of Jacobi (1991) and Karcher and his colleagues (2006) and maintains the necessary theoretical alignment proposed in recent literature (Hall & Jaugietis, 2011; Crisp & Cruz, 2010) within a holistic and integrated institutional approach to student success and retention.
Assessment of Energy Use and Renewable Energy Growth Potentials in Utah
Buchanan Kerswell, Utah Valley University Academic Affairs Increase in population and wealth in Utah will likely result in increased fossil fuel consumption. Consequently it will lead to more environmental problems, especially air quality and health issues. It is essential to further the research and assessment of renewable energy sources. The objective of this research is to compile, analyze, map and assess energy data from the Utah Geological Survey, Utah Office of Energy Development and Utah Department of Environmental Protection. Our project will give public a clear picture of air quality and energy use coupling with population and economic growth. ArcGIS maps and statistical analysis will be made using the available database. Furthermore, detailed assessments on the development potentials of different renewable energy sources (e.g. solar, wind, geothermal) in Utah will be conducted using ArcGIS. Our preliminary data analysis on fossil fuels indicates that consumption and expenditures have grown over time with population growth. A notable fact is that when expenditures have risen rapidly, consumption tends to decline. The most recent evident periods are during the early 1980s and in the early 2000s, when oil prices were rapidly increasing. Furthermore, the data show that air quality is closely correlated with the quantity of fossil fuel consumed, especially given Utah’s special topography (the valley effect). The data on renewable energy sources have revealed that all renewable energy sources together provide less than 1% of energy need in Utah. Although the growth and development have varied during the last half century, there is a steady growth in geothermal, solar and wind energy over the last decade. Results from ArcGIS mapping will provide useful insights on zoning and assessing potential renewable energy sources in Utah. Renewable energy is the key to our economic growth and clean air in Utah. It is essential that the transition from a primarily carbon-based energy portfolio be to one that includes a greater mix of renewable sources. Further results, analyses and maps will be presented during the meeting.
“Lloyd Alexander”: A Documentary Film
Jared Crossley, Brigham Young University Education This research project was to make a film about Lloyd Alexander, one of America’s most influential authors of fantasy for young readers. Best known for his Chronicles of Prydain, including the 1969 Newbery Award winning “The High King”, Alexander won numerous awards and was translated into over 20 languages. This study includes interviews with his editors, close friends, and professionals in the children’s literature community as well as archival photographs from his life. The research actually culminated in two documentary films exploring the life and writings of Lloyd Alexander, one ten minutes long and the other an hour in length. These films already have created much interest in Alexander and his works, reaching long-time Alexander fans, as well as helping to establish some new readers of his work.
A Ponzi in Sheep’s Clothing
Tyson Jones, Utah Valley University Academic Affairs In 2008 the people of the United States and the world were awakened from a dream that huge financial gains can be made at the expense of huge public losses while everything will stay just and fair. Unfortunately, the 2008 economic crash wasn’t the result of just one idea in one sector, but rather a culture within economies; a culture that has not just investment bankers but everyday American and foreign citizens pursuing the same path. This path, that many American and foreign citizens have been enticed by, is a business structure that leaves an individual and their chances of success equal to the chances of failure of those that follow suit, namely Ponzi Schemes. The many people who follow Ponzi Schemes are looking for financial security along with promises of high earnings and early retirement, but are often met with greater debt than they had before simply due to the structure of these Ponzi schemes. For the most part Ponzi schemes have been made illegal in the United States; however, due to certain laws that have been established, Ponzi schemes have been operating under the guise of established businesses known as Multi-level Markets (MLMs). The laws in question are the DSHEA, which has aided MLMs on a national level, and Utah S.B. 0182, which created a loophole for MLMs to operate in large numbers on a State level. Thus a culture of legal positivist views allows the illegal and immoral business practices of MLMs to encourage a culture of harming many to reward a few. This conclusion about MLMs comes from contrasting the structure of MLMs with the moral theory of Immanuel Kant’s Categorical Imperative which requires that all moral actions be held as a universal maxim, and that people are not treated as merely means to an end. The purpose of this research is to assess whether the previous conclusion is in fact true of MLMs in regards to moral business practices through the Categorical Imperative. If after examination the conclusion is found to be true, the DSHEA and Utah S.B. 0182 both need to be revoked in order to begin a change in culture; else large amounts of U.S. dollars, as well as other currencies, will continue to fund illegal Ponzi schemes acting as legitimate businesses and an opportunity to take steps to change U.S. culture on the nature of wealth. In addition, a new culture that allowed for the 2008 financial crisis of large rewards for the few at huge public expenses will continue to grow.
Human and Domestic Influences on Vegetative Communities Surrounding Capitol Reef Field Station
Megan Curtis, Utah Valley University Academic Affairs Capitol Reef Field Station (CRFS) is located in south-central Utah within Capitol Reef National Park (CARE). Due to its arid climate and diverse geology, many plant species found within CARE have unique adaptations that are sensitive to disturbance. Cryptobiotic crusts, which play a vital role to the health of vegetation by stabilizing soils, cycling nutrients, and reducing erosion, are extremely sensitive to disturbance and can take many years to recover after being damaged by footprints. In addition, the spread of invasive species can harm native vegetation by competition for resources. Since CRFS’s founding in 2008, it has been frequented by visitors who come to learn about CARE’s natural and cultural history. Consequently, various trails and dirt roads surrounding CRFS may be a source of human disturbance and spread invasive species. Another source of disturbance is cattle that graze in this area on their route through CARE twice each summer. The objective of this study is to characterize the vegetation surrounding CRFS and determine the present level of human and domestic impact on this vegetation. Our specific questions were; (1) Is the level of human disturbance associated with plant community structure and proximity to CRFS, (2) Which communities have the highest levels of cattle/human disturbance?, (3) How does community structure vary by vegetation type? To address these questions, two 100-m transects with differing proximities to CRFS were established in each of four vegetative communities’ Pinyon-Juniper, Big Sagebrush, Riparian, and Grassland. Species frequency, cover, and disturbance (density of tracks within a quadrat) were recorded within each transect using a nested plot frequency design -four nested subplots ranging from 0.25 4m2. To account for seasonal variance, data were collected in four sampling periods throughout growing season. To characterize community abiotic factors, slope, aspect, and soil attributes were measured for each transect. To analyze the data, we ran multivariate analysis, including Non-metric Multidimensional Scaling (NMS), and Multi-response Permutation Procedure (MRPP). The two-dimensional NMS solution explained 80.1% of variability in community structure.
An Assessment of the Amount of Physical Education Class Time in Utah Schools
Chase Hansen, Utah Valley University Academic Affairs Purpose
A Magnificent Endowment: America’s School Trust Lands
John Maynes, Utah State University The Center for the School of the Future In the early history of the United States, the Founders of our nation set aside vast amounts of land which were to be used to support public schools. Starting in 1785, lands were dedicated to support schools and thereby provide public education to new settlers in the “Northwest Territory.” Further legislation in 1787 reinforced this granting process with the language, “Religion, morality, and knowledge being necessary to good government and the happiness of mankind, schools and the means of education shall forever be encouraged.” These actions were championed by many of the same men who would be influential in writing the Constitution, most notably Nathan Dane of Massachusetts. However, after the first lands were granted, many new states were admitted to the United States without granting lands for schools. It seemed that the idea for granting school lands had been forgotten. However, in 1803 Ohio was admitted as a state, and the land grants for public schools were renewed. Since that time, every state admitted to the Union was granted school trust lands. The purpose of this study is to address the following questions: What resources were used to support public education? How did attitudes toward these resources evolve from the beginning of our country to now? Based on preliminary findings, we can see that attitudes towards school trust lands have varied throughout history and the policies and resources used to support them have been equally variable. By looking at school trust lands and school permanent fund values, I hope to evaluate the attitudes of decision makers toward education over the course of American history. Using historical records such as legislation, Congressional annuls, and personal correspondences to evaluate personal attitudes of policymakers, this study will identify determinants that supported the unique attitudes and policies surrounding school trust lands.
Gene Expression in Arabidopsis thaliana In Response to a Growth Enhancer
Keely Glade, Utah Valley University Academic Affairs Cytozyme Laboratories, Inc., Salt Lake City, UT is an agricultural company that provides a range of products for large food crop farming and animal production. In order to provide data to prospective buyers on their products’ effectiveness in improving crop yields and health, they chose to study the expression levels of several different genes using Arabidopsis thaliana as their model organism. These genes include: 1) At2g14610 encoding Pathogenesis Related gene 1 (PR1), which assists in plant pathogen defense, 2) At2g29350, Senescence-Associated Gene 13 (SAG13), and 3) At2g30770, Cytochrome p450, Family 71, Subfamily A, Polypeptide 13 (CYP71A13). A. thaliana leaves were treated with a proprietary substance implicated in plant health and potential crop yield. Treated and untreated leaf homogenates were used to create cDNA from purified RNA for the quantitative real-time PCR (qRT-PCR) assays. The qRT-PCR assays were performed on the cDNA generated from PR1, SAG13, CYP71A13, with the 40S ribosomal subunit as the internal control, on an ABI 7500 Fast Real Time System using gene-specific primers and SYBR green as our reporter molecule. The fold change, a value that is a relative comparison between the non-treated and treated samples, for each gene was calculated. The results showed clear up regulation of PR1, SAG13, and CYP71A13 compared to the 40S ribosomal subunit internal control. Future experiments include qRT-PCR and microarray analyses for 11 other proprietary treatments provided by Cytozyme.
Beauty and the Advertising Beast: The Sales Implications of Representing Real Women in Advertising
Hallmat Ipaye, Westminster College Marketing Marketers and advertisers allocate a compelling amount of resources to deciphering their target market, however, currently many women express that advertisements targeted towards and portraying women do not represent real women. An increasing disconnect exists between what an average woman actually looks like, thinks, acts and does and how a woman is marketed to in advertisements, specifically in women’s fashion and beauty magazines. Advertisers and marketers make important decisions regarding advertising and marketing without first consulting consumers about finished advertisements. Studies have shown that women do not relate, and often have lowered self esteem after looking at modern fashion and beauty magazines. This research and presentation focuses on categorizing what is important to women over the age of 18 to gain insight on how advertisers and marketers can better represent women in the advertisements of popular fashion and beauty magazines. July 2012 issues of fashion and beauty magazines Vogue, Glamour and Cosmopolitan will be discussed in terms of presence of factors that are important for women to relate to the advertisements in these magazines. The conclusion of these findings will further demonstrate the sales implications of representing real women in advertising from a survey of over 200 women.
How Estonia Became an OECD Country
Colin Cox, Weber State University Economics Acceptance into the Organization for Economic Co-Operation and Development (OECD) can be interpreted as a sign that a country has achieved a level of high economic development. The pathways leading to becoming an OECD country are as diverse as the countries within this organization. The most recent country to join this elite organization is the small eastern European country, Estonia. Besides being the most recent country to be inducted into the OECD Estonia has another unique characteristic, it is the only OECD country to have belonged to the Soviet Union. Estonia’s relatively new independence gives researchers an extraordinary opportunity. We are able to track this country’s economic progress after its policies and institutions were essentially wiped clean upon gaining independence in 1991. In this study I will investigate what macroeconomic devices Estonia has used to progress further than other former Soviet States. In order to do this I will compare key economic indicators and policies for three former Soviet countries with similar economies; Estonia, Latvia, and Lithuania. I will draw connections between economic policies implemented and correlating empirical indicators. After analyzing the above mentioned parameters I will provide a summary of the successes and shortcomings experienced by Estonia’s economy and make relevant suggestions.
Collective Cost Economics through a Progressive Era Lens
Mimi Marstaller, University of Utah Economics During the Progressive Era that stretched roughly from 18771928, the United States faced the rising costs of industrialism. As corporate capitalism expanded and a national market replaced local economies, the role of the federal government changed to include mitigating collective costs by providing public goods. The Progressive Era represents the U.S.’ first national discussion on the role of the state in an industrialized nation. The nation, newly linked by economic ties, faced a collective action problem. I use this historical backdrop to examine economic theories on how societies manage the costs of capitalism. I look at scholarship on collective action, spontaneous order, self-regulation and enforcement within economic systems, and explore how the Progressive Era exemplify or refute the theories on social and economic behavior. I trace the emergence of a national market from pre-industrial, local economies, and examine how in the national context citizenship, the newly powerful nation state and standardized treatment of collective costs played an essential role in establishing the market as the central motivating factor of 20th century American culture. The institutions supporting citizenship and facilitating market participation bolstered capitalism but also required significant oversight by the regulatory bureaucracy established during the Progressive Era. While mainstream economic theory minimizes the role of class interests and power dynamics by assuming freedom of contract and costless enforcement of property rights, recent work in economics invokes sociology and history to understand the barriers to collective action. Today’s most illuminating theories revive the thought processes of the early, classical political economists, and I use the Progressive Era to explore the extent to which their theories on collective cost management describe the case of U.S. industrialization.
The Energy Policy Act of 2005: The Case of Solyndra
Megan Hansen, Utah State University Economics This research represents one chapter out of a larger book written with the help of fellow student researchers at Liberty Source under the direction of Dr. Randy Simmons. The book itself questions the notion common among environmentalists that a balance of nature exists and that governments should take steps to restore that balance when it is upset. This research in particular examines the effects of the Energy Policy Act of 2005, and the subsequent funding of alternative energy start-ups by the federal government in an effort to restore the balance of nature. It includes a detailed case study of Solyndra, a solar power manufacturing company that received a large federal loan from the Department of Energy under the Obama administration only to default a few years later. This case study questions the role of the government in “picking favorites” when it comes to alternative energy, and argues that failure is likely when this occurs due to imperfect information and the tendency for politics to play too large a role in decision-making.
Preparation, Acculturation, and Repatriation: A Mixed Methods Approach to Understanding the Mormon Missionary Experience
Joshua Blume, Utah State University Economics and Finance Studies of expatriates in a number of industries have suggested that acculturation plays a role in how individuals re-adapt to their home country after working abroad. This study applies acculturation and repatriation frameworks to returned Mormon Missionaries (who have also spent significant time away from home and family). Qualitative and quantitative results suggest that re-adaptation to home, family and school are affected in part by cultural components of the mission experience. Recommendations are made to assist “expatriate missionaries” in the re-acclimation process.
Hybrid Social Enterprise Businesses as a Rising Business and Marketing Strategy
Scott Hoopes, Dixie State University Business Hybrid Social Enterprise Businesses are companies that sell products and donate a portion of their proceeds to a social cause. I submit that these types of businesses will become the patterns of marketing and business organization of the future because they are able to fund their social undertakings from the revenue of the products they sell, and be sustained by sales rather than depending on donations to fund their endeavors. The reason this model is successful is because many people throughout the world want to make a difference but rarely take action to put that change into effect. Hybrid Social Enterprise Businesses are able to make a difference in the world through their donations yet they have their revenue from product sales to run the day-to-day business. These items such as TOMs shoes, People Water, and comparable companies are becoming status symbols for socially conscious people worldwide. Not only are these items “fashionable” or “stylish” they are giving consumers the feeling that they have made a difference in somebody else’s life by making a purchase. By comparing and analyzing data from a variety sources and primary research I will be able to support my hypothesis that Hybrid Social Enterprise Businesses are a growing trend of business organization and marketing and will continue to grow into the future.
The Influence of Culture on Freedom of Information in the United States and United Kingdom
Whitney Evans, Brigham Young University Communications The United States adopted a freedom of information law earlier than the United Kingdom, but the latter has surged ahead with its adoption of an Information Commissioner’s Office, well equipped to handle complaints and mediate in complex situations. This article delves into the attitudes of those who are intimately involved with freedom of information laws: journalists, lobbyists, campaigners, advocates and government officials. This qualitative research showed a similar attitude at a government level in both countries, namely, a hesitance of government officials to warm up to the law. The Information Commissioner in the United Kingdom and the Office of Government Information Services in the United States are essentially limited to recommendation and mediation services. The United States, initially progressive in its open government laws, has lagged behind many other nations in terms of disclosure. Because the law is nearly 50 years old, the Freedom of Information Act here is often taken for granted. Complacence, rather than outright defiance, obstructs successful implementation of this law. The United Kingdom has the lingering legacy of an Official Secrets Act obstructing what would otherwise be a clean slate on which to build their government disclosure laws. The key to successful utilization of freedom of information laws in both countries lies in a shift in each country’s culture, instigated by a marked change in the stories being told.
California: Balancing Energy Extraction with Natural Amenities
Kelsey White, Utah State University Economics and Finance California is endowed with some of the United States’ most beautiful natural landscapes. It also lies atop significant energy resources. While preserving natural amenities and developing energy are sometimes considered mutually exclusive endeavors, the reality is that most counties throughout California have developed both of these rich resources. While the ratios between amenities and energy differ, almost all counties with available opportunities have developed both to some extent. This paper compares and contrasts the balance between energy and amenities in three California counties. Monterey County is economically focused on agriculture and amenities, but has a strong potential for developing its shale resources, and some extraction has already begun. Ventura County also boasts plentiful natural amenities, but engages in significant oil production, particularly offshore production. Kern County is economically dependent upon oil extraction, but still maintains an active amenity sector. The fact that all of these counties have opted for a mixed economic portfolio balancing energy and recreation demonstrates that the two activities are not mutually exclusive, but rather that counties already opt for some mixture of the two. The three counties are compared on several key economic indicators such as per capita income and unemployment by using compiled US Census Bureau data. Counties with a mixed economic portfolio enjoy higher economic outcomes than those counties that focus more exclusively on either natural amenities or energy extraction.
Measuring Cellular Ceramide Accrual using Immunofluorescence
Anindita Ravindran, University of Utah Exercise and Sport Science Obesity predisposes individuals with Type II Diabetes to cardiovascular complications such as impaired blood vessel function. Due to the elevation of free fatty acids (FFAs) in obese individuals, ceramide, a lipid metabolite, accumulates and might contribute to the inability of a blood vessel to constrict or relax appropriately. Vessel dysfunction is partly caused by the inability of the endothelium, the innermost protective lining of blood vessels, to synthesize and release nitric oxide (NO). Our data indicate that ceramide impairs endothelial NO synthase (eNOS), the enzyme that synthesizes NO. In order to study mechanisms by which ceramide might impair eNOS, it is important to measure cellular ceramide production in response to pharmacological and genetic manipulations. Previously we used P-32 radioactive assays to measure ceramide accumulation. However, the use of radioactivity is expensive, potentially hazardous, and waste disposal is an environmental concern. Therefore, I sought to import a less harmful, more cost effective, yet accurate technique of measuring ceramide production by immunofluorescence (IF). IF allows ceramide to be tagged with a primary antibody which can be detected by a secondary antibody conjugated with a fluorescent dye. I have observed that 250, 500, and 750 uM palmitate (pal) incubation for 3 h increases (p<0.05) endothelial cell ceramide accrual in a dose-dependent manner. Further, a FFA-independent method to alter ceramide accrual i.e., 3 h incubation of cells with N-oleoylethanolamine, also elevates (p<0.05) ceramide production. Importantly, I have shown that 500 uM palmitate-induced ceramide accrual can be prevented (p<0.05) by two structurally dissimilar inhibitors (10 uM myriocin, 1mM L-cycloserine) of the rate-limiting enzyme responsible for ceramide biosynthesis i.e., serine palmitoyl transferase (SPT). None of these inhibitors impairs cell viability. These data indicate that IF is an accurate and reproducible method whereby ceramide accrual can be quantified in endothelial cell systems.
The Effect of Non-tutoring Social Interaction on Learned Birdsong
Jason Chen, University of Utah Biology Both human language and birdsong are learned sensorimotor processes that require exposure to adult communication signals in order to be reproduced. The social aspect of the development of these vocal signals is distinctive because interaction is not required for the development of vocal signals in other organisms. Human infants isolated from interaction will not learn to speak normally. The need for interaction is also characteristic of the ontogeny of vocal signals in the zebra finch (Taeniopygia guttata), but only zebra finch males develop vocal signals, in the form of song. Juveniles raised in isolation will develop highly atypical song characterized by prolonged, monotonic syllables and irregular rhythms. When juveniles are tutored by these isolated birds in one-on-one conditions their song drifts towards normally raised birds. It is unclear how tutoring, as a social interaction, influences this observation. We hypothesized that different social experience influences birdsong. We predicted the song of juveniles raised in isolation with an experienced female to be closer to normal song than that of those raised in isolated sibling groups, or those raised in isolation. Current data suggest that the syntax and temporal features of song from juveniles raised in isolation with experienced females is indeed closer to normal song than that of isolated juveniles.
Dynamic Microtubules and Endomembrane Cycling Contribute to Polarity Establishment and Early Development of Ectocarpus Mitospores
Diego Cordero, University of Utah Biology Many zygotes and spores of brown algae are photosensitive and establish a developmental axis in accordance with directional light cues. Ectocarpus siliculosus is being advanced as a genetic and genomic model organism for investigating brown alga development, and this report investigates photopolarization of the growth axis of mitospores. When exposed to unidirectional light, mitospores photopolarized and established a growth axis such that germination was preferentially localized to the shaded hemisphere of the spore body. The roles of the microtubule cytoskeleton and endomembrane cycling in the photopolarization process were investigated using pharmacological agents. Disruption of microtubule dynamics progressively reduced the percentage of mitospores that photopolarized, while inhibition of vesicle secretion blocked photopolarization nearly completely. Chronic treatment with these pharmacological agents severely affected algal morphogenesis. Microtubules in mitospores and algal filaments were imaged by confocal microscopy. Mitospores contained a radial microtubule array emanating from a centrosome associated with the nuclear envelope. At germination, the radial array gradually transitioned into a longitudinal array with microtubules extending into the emerging apex. At mitosis, spindles were aligned with the growth axis of cylindrical cells in the filament, and the division plane bisected the spindle axis. These studies demonstrate that dynamic membrane cycling and microtubule assembly play fundamental roles in photopolarization, and provide a foundation for future genetic and genomic investigations of this important developmental process.
Strength in Numbers: Behavioral Response of Speckled Dace
Travis Wood, Southern Utah University Biology Speckled dace, Rhinichthys osculus, is a small minnow that occupies river systems in Utah, Arizona, and Nevada. These minnows prefer clean, faster-moving water with plenty of cover such as rocks or undercuts. The invasive crayfish Orconectes virilis inhabits areas where the Virgin spinedace is found and has a similar diet being an opportunistic feeder. I tested the hypothesis that the natural behavior of Virgin spinedace will be altered due to the presence of crayfish. I predicted that the spinedace would avoid the crayfish and stay out in the open exposing them to a higher risk of predation, since crayfish are a more aggressive organism and will try and eat virtually anything they come into contact with. The experimental trials were conducted (N=24) in Quail Creek, Utah. Large plastic bins with the front and back replaced with screen were used to allow water flow through our experimental chambers. Each bin was placed in the creek and had rocks, and an undercut bank to resemble the native habitat. Controls consisted of placing isolated Virgin spinedace in one of the bins to observe its behavior. In both controls and experimental trials, either 2 spinedace, or 5+ spinedace were added. In experimental trials, I introduced 2 crayfish into the experimental chambers with the Virgin spinedace and allowed them to acclimate for 10 minutes. After the 10 minute acclimation period, behavioral observations were made. Results indicated that when 2 spinedace were present along with the crayfish, they would stay out in the open and the crayfish would occupy the cover or undercut. When 5+ spinedace were present with crayfish, the spinedace displayed a mobbing or “strength in numbers” behavior where they would occupy the undercut and either clog it up with their presence so the crayfish would leave, or mob the crayfish to make them leave. This behavior was opposite of what I predicted would happen. Since this mobbing or “strength in numbers” behavior was not observed with the samples of just 2 spinedace, this may be a behavioral adaptation resulting from the presence crayfish. Further research can be done on the behavioral effects of various fish species in the presence of introduced or invasive species to determine if this behavior is seen in other fishes.
A New Approach to Creating Pradimicin-Type Antifungal/Antiviral Compounds
Thomas Anderson, Utah State University Biology Pradimicin, a small molecule produced by the soil bacterium Actinomadura hibisica, is a promising candidate as a combined antifungal/antiviral therapeutic. It is active against a broad-spectrum of opportunistic, pathogenic fungi, interferes with the replication of influenza virus, and inhibits the reproduction of HIV-1. Toxicity and solubility problems have hindered past efforts to develop pradimicin as a therapeutic. Our research focuses on elucidating the bio-synthetic pathway of pradimicin in order to design and chemoenzymatically create pradimicin structural analogs with improved solubility and activity, and less toxicity. Several enzymes in pradimicin biosynthesis have been identified. We intend to characterize one of the key enzymes, PdmS, a putative glycosyltransferase, and to manipulate its gene to create novel, more efficacious pradimicin analogs. This project is funded for three years by the NIH NIAID (3 years). Methods: A gene knockout experiment was used to determine the role of PdmS in pradimicin production. Bio-synthetic precursors of pradimicin were subjected to bio-transformations in E. coli with recombinant genes for PdmS and another glycosyltransferase, OleD, to generate analogs with new sugar attachments. Analogs of pradimicin will be screened for bio-activity using standard microbroth dilution assay techniques. Confirmed results: The enzyme PdmS was identified and characterized as a glycosyltransferase. Expected results: newly created analogs of pradimicin exhibited minimal inhibitory concentrations of 10μg/mL against Candida albicans. Conclusion: Knockout of pdmS yielded the pradimicin aglycon, which confirmed the function of this glycosyltransferase and provides a start molecule for further structural modification to yield new analogs for bioactivity studies.
The Role of Aggression in the Evolution of the Human Hand
Joshua Horns, University of Utah Biology There are numerous arresting differences between the hands of humans and those of chimps, principally among them the shortening of metacarpals 2-5, the relative increase in length of the thumb, and the development of separate musculature controlling flexion of the thumb. In addition to improving manual dexterity, these changes allow humans, in contrast to chimps and other apes, to make a buttressed fist with the phalanges pressed against the central palm and the thumb adducted onto the dorsal surface of the phalanges. We believe this hand posture greatly reduces the strain experienced by a fully formed fist when striking with force, thereby rendering the human hand as a more effective weapon. We tested this idea by manipulating the tendons in a cadaver arm to induce the hand to form into buttressed and non-buttressed conformations, and then tested each conformation by having the hand strike a hanging instrumented mass to measure the force of each strike. Additionally, the 2nd metacarpal of the hand was fitted with a strain gauge so that strain in the bone could be compared to the force of the strikes. The results of these tests showed that there was a significant drop in strain in the buttressed fist (in comparison to the non-buttressed) for a given force.
Vascular Function Assessed During Handgrip Exercise Following Heart Transplantation
Alexa Provancha, University of Utah Exercise and Sport Science Impaired endothelium-dependent vasodilation has been associated with various cardiovascular diseases, including heart failure, is linked to oxidative stress, and persists despite heart transplantation (HTx). PURPOSE: This study aimed to determine if changes in vascular function following HTx can be assessed using incremental handgrip (HG) exercises to induce nitric oxide-dependent vasodilation of the brachial artery. Furthermore, the efficacy of an acute oral antioxidant cocktail (AOC) to reduce oxidative stress and improve vascular function in this population will be assessed. METHODS: 31 HTx recipients (14 years post-HTx) and 10 healthy age-matched controls were given either and AOC (Vitamin C, E, and alpha-lipoic acid) or placebo (PL; randomized for the subjects’ two visits. Measurements of the brachial arterial blood velocity and vessel diameter were performed during three absolute workloads (4, 8, and 12 kg) of HG exercise using ultrasound Doppler. RESULTS: Maximal vasodilation during PL HG exercise was not different between the controls (8.2 ± 1.5%), and recent (< 3 years post) HTx group (8.5 ± 1.2%), but the 5-10 years post-HTx groups had a tendency to be lower (6.5 ± 1.9%). The > 14 years post-HTx group recipients (5.2 ± 1.9%) were significantly attenuated compared to both the controls and the recent (< 3 years post) HTx recipients. CONCLUSION: The study supports the use of HG exercise as an assessment of vascular function in a patient population with known cardiovascular risk. Additionally, these results suggest that vascular function is similar between controls and early HTx recipients but declines as time passes following surgery despite normalized cardiac function.
Distinguishing Kentucky Bluegrass Varieties Using EST and Genomic Primers
Kat Combs, Utah State University Plants, Soils, and Climate Kentucky bluegrass (Poa pratensis L.) is a commonly used turfgrass species with many varieties being sold around the world. However, those varieties are very difficult to tell apart morphologically. Our objective was to use genetic markers (primers) to identify varieties, even if they are visually similar. This is valuable to the turfgrass industry for plant variety protection. We also wanted to use this data to explore the apomictic tendency (clonal reproduction) of the varieties. We collected leaf tissue from 24 Kentucky bluegrass varieties, extracted DNA, and sequenced portions using 29 EST and 21 genomic primers. This data was used to determine genetic relationships using a neighbor-joining dendrogram. Similarities of the genetic sequences from the varieties were estimated using the DICE coefficient. We found more polymorphisms in genomic primers than in EST primers with high variability between the varieties. Both types of primers were robust enough to distinguish varieties and that each variety was unique and genetically distinguishable. In addition, we discovered some varieties had large amounts of variation within a variety. This was unexpected due to the usual apomictic nature of the species. The markers resulting from our research will be available to the turfgrass industry.
Characterizing the Properties of Cell Volume Regulation in Retinal Neurons and Glia: A Role for the Mechanosensitive Cation Channel TRPV4
Andrew Jo, University of Utah Ophthalmology and Visual Sciences All cells, including retinal neurons and glia, must sense and adapt to physical changes in their local environment (e.g. changes in osmotic pressure). Osmotic water flux can cause aberrant cell volume changes, which can contribute to tissue damage, edema, and neuronal hyperexcitability and excitotoxicity. We hypothesized that force-sensitive proteins enable retinal cells to monitor their physical form (e.g. volume) and help maintain homeostasis by regulating cell volume. To test this, we first investigated the properties of cell morphology when cells were bathed in solutions with different tonicities. Under these conditions, we measured changes in cell volume due to osmosis in retinal ganglion cells (RGCs) and Müller glia. We found RGCs were unable to actively adjust their volume, whereas Müller glia reduced their swollen volume in the continued presence of hypotonicity. The regulation of cellular volume often involves calcium signals. We tested whether calcium plays a role in the regulation of retinal cell volume. Free calcium within the cells was sequestered using cytosolic BAPTA, which decreased the extent of hypotonic swelling. This demonstrates that calcium elevations increase the extent of cell swelling. Because cell volume was dependent on calcium, which was elevated by membrane stretch, we hypothesized that the osmosensitive cation channel TRPV4 would transduce osmotic pressure and contribute to cell volume regulation. In an experiment using a selective TRPV4 inhibitor, the extent of hypotonic-induced swelling was reduced. Thus, the opening of TRPV4 leads to a calcium influx that exacerbated cellular swelling. In addition, we tested the idea that TRPV1 cation channel is involved in responses to osmotic stimuli. In an experiment using a selective TRPV1 antagonist, preliminary results show that the extent of hypotonic-induced swelling decreased. This signals that TRPV1 may have a role in volume regulation in retinal neurons and glia. Thus, inhibition of these force-sensitive protein channels might alleviate the deleterious effects of volume changes in pathological contexts. Our findings therefore have implications for our understanding of retinal mechanotransduction and osmoregulation as well as provide a mechanistic framework for developing new therapeutic strategies aimed at blinding conditions that involve mechanical stress and cellular morphology.
Does Gamma-Tocopherol Lower Blood Pressure in Diabetic Mice?
Makenzie Hawkins, University of Utah Nutrition Cardiovascular disease (CVD) is the leading cause of death in the United States. Gamma-tocopherol (GTC) has been studied in its relation to hypertension and CVD. GTC has protective effects on the endothelium of arteries; the endothelium is in part responsible for regulating arterial relaxation and reducing blood pressure (BP). Diabetic mice (DB) were chosen for this study because of the associated co-morbidities of hypertension and excess body weight. Eight-week-old mice were divided into 3 separate groups: wild-type fed control (CON) diets (n=12), DB fed CON diets (n=12), and DB fed GTC supplemented diets (0.1%, n=12). BP was measured every week for 8 wk. Body weights were measured every wk and morphometric values were measured at the end of the 8-wk period. There was no statistically significant (P<0.05) difference in systolic and diastolic BP between DB-GTC and DB-CON. There was also no statistically significant (P<0.05) difference in heart rate between DB-GTC and DB-CON. After 8 wk, DB-GTC mice had lower (P<0.05) body weight versus the DB-CON group. In conclusion, a GTC supplemented diet did not result in a change in BP. However, GTC may have blunted weight gain and adipose tissue mass in DB-GTC compared to DB-CON. We speculate this may be due to a reduction of food consumption during the experimental period.
SNAP-25 Neurotransmission in C. elegans
Viktor Jiracek, University of Utah Biology The nervous system is responsible for cognition, memory, and motor function. Neurons communicate with each other at intercellular connections called synapses. It is at these locations that vesicles fuse with the plasma membrane and release their contents into the space between the neurons (synaptic cleft). SNARE proteins (synaptobrevin, syntaxin and SNAP-25) facilitate synaptic vesicle fusion by winding together in a four-helix bundle forcing the mixture of opposing membranes. After exocytosis, the cell regenerates vesicles by a process called endocytosis. This process involves the budding of membrane back into the cytoplasm re-forming a functional synaptic vesicle. This progression from vesicle fusion to internalization is termed the “synaptic vesicle cycle” and is coordinated by a long list of molecular players. The SNARE proteins are classically considered to be unique to exocytosis. However, preliminary results from Erik Jorgensen’s lab have implicated SNAP-25 in endocytosis. Although we have evidence that SNAP-25 is required for endocytosis, the molecular mechanism is completely unknown. In this proposal we use forward genetics in the model organism C. elegans to identify novel protein interactions required for SNAP-25 mediated endocytosis. We have designed three suppressor screens that are predicted to target the role of SNAP-25 in exocytosis, endocytosis and general function. The first two screens use crippled forms of SNAP-25 (hypomorphs) while the last screen uses a deletion allele of the SNAP-25 gene (null mutation). With these screens we hope to precisely identify the molecular players involved in SNAP-25 mediated endocytosis versus exocytosis.
Rationally Modified Tumor Suppressor Protein p53: A Possible New Cancer Treatment
Thomas Wallace, University of Utah Pharmaceutics and Pharmaceutical Chemistry The tumor suppressor p53 is mutated in more than 50% of all cancers, while the majority of remaining cancers contain mislocalized p53(1). p53 is part of a network of cellular pathways that regulate growth, cell cycle arrest, and clearly delineated apoptotic pathways(2). Restoring the function of p53 can be seen as an ultimate cancer treatment. Restoring p53 would activate an already existing cell pathway that prevents cells from becoming cancerous and cause cancer cells to go through apoptosis. Playing a role in tumor suppression has made p53 an attractive target for gene therapy. However, despite the potential of p53 as a powerful treatment, it is limited by the dominant-negative effect of dysfunctional mutant p53. This effect imposes one of the greatest limits on the effectiveness of a p53 based treatment. This project is focused on bypassing the dominant-negative effect of dysfunctional p53 over exogenous functional p53. The attempted solution was substitution of the p53 binding domain with a different but structurally analogous coiled-coil, based on a modified Breakpoint cluster region (Bcr) protein. By doing this, the dominant-negative effect of mutant p53 may be bypassed. The purpose of this project has been to synthesis and test rationally modified forms of p53 with modified Bcr coil that are introduced into cells via a plasmid to restore cellular p53 activity. In vivo cell tests have already shown the effectiveness of these constructs at causing higher rates of cell death in cancer cells and constructs are currently being refined to carry forward to xenograft model animal trails. The ultimate goal is to develop a treatment for human cancer patients where modified p53 will selectively cause apoptosis in cancer cells.
Comparing Trophic Level Position of Invertebrates in Fish and Fishless Lakes in Arctic Alaska
Katie Fisher, Utah State University Watershed Science Arctic lakes are very sensitive to the effects of climate change. It is important to understand the current food web dynamic and energy flow within these lakes to better understand how they will change in the future due to the effects of a rapidly changing climate. In order to understand the current conditions in arctic lakes, this project consists of an analysis of stable isotopes of carbon (13C) and nitrogen (15N) from invertebrates among fish and fishless lakes in arctic Alaska to compare their trophic level positions and primary energetic sources. In order to carry out this analysis, I collected pelagic invertebrates were collected from 6 different lakes, 3 of which have resident fish populations and 3 of which are fishless. Samples collected in 2011 were analyzed for stable isotope composition by a mass spectrometer at University of California Davis. Results from samples collected in 2012 are pending at Washington State University. I will analyze and correlate the stable isotope results with isotopic data collected from other related projects. With this analysis, I will create food webs to 1) assign trophic positions to each species in each lake and compare those positions across lakes, 2) assess the potential effect fish predation has on pelagic invertebrate community structure. I hypothesize that fish predation will determine zooplankton community structure (e.g., dominant taxa) and alter trophic linkages (e.g., secondary trophic level predation rates).
Fluorene as a Model Compound to Investigate Fire Induced Soil Water Repellency: A qualitative approach
Vance Almquist, Utah State University Plants, Soils, and Climate Fire induced soil water repellency has been characterized across a variety of soils and landscapes as being a cause of watershed degradation and surface water pollution. The repellency is due to the condensation of volatile polyaromatic hydrocarbons onto soil particles. Although repellency is known to reverse, in some locations the reversal takes months; whereas, in other locations it may take years. Little is known about the reversal mechanisms and how they lead to such a large range of reversal times. Access to untreated fired-affected sites, can be unpredictable and samples vary greatly from site to site. Therefore, a model compound that mimics fire-induced soil hydrophobicity is needed to be able to systematically investigate soil hydrophobilicty reversal mechanisms. Fluorene is a relatively non-toxic, hydrophobic polyaromatic hydrocarbon. The behavior of fluorene coated sand grains was investigated under laboratory conditions using quartz sand. Moreover, its fluorescent properties could be used to non-destructively monitor its degradation over time. In this context fluorene was studied as a possible model compound for the study of mechanisms involved in the reversal of fire-induced soil hydrophobilicy. The compound was subjected to conditions known to degrade or reverse water repellency including temperature, UV-light, and moisture content. Changes in hydrophobicity were monitored using the ethanol drop test and fluorescent imaging. Digital image processing techniques with the public domain software, ImageJ, produced by the National Institute of Health, were used to analyze the images and generate spatial maps of treatment effects on fluorene degradation and hyrdophobicity reversal. Our results indicate that the hydrophobic fluorene-coated sand layers were degraded by treatments such UV light known to reverse hydrophobicity in fire-affected soils, thus suggesting that fluorene may serve as a suitable model compound for producing hydrophobic layers on course grained material.
Identification of Genes Regulating Sperm Motility in C. elegans
Kandrie MyIroie, University of Utah Human Genetics In C. elegans, sperm activation is the process through which sperm become motile. Genes that regulate sperm motility are being identified through a genetic screen. In this screen, swm-1 mutants that have sperm that activate too early are mutagenized, and genes regulating sperm motility can be found when the sperm of mutant worms no longer activated too early. Two genes have already been identified, try-5 and snf-10, and these can help identify new mutations. By selecting specific strains with sperm activation defects, the mutations causing the defect can be characterized. To sort through the many mutants, complementation tests to identify them as a new gene, or a new allele of try-5, snf-10 are being done. Selectively crossing an unknown mutant strain to a strain with a known mutation in try-5 or snf-10 will help identify the unknown mutation. If the progeny of the cross have activated sperm then the two strains complement each other. In this case the alleles must be in different genes and the unknown mutation from that cross has not been identified. If the progeny instead has non-activated sperm then the mutations failed to complement and the unknown mutation is allelic to the mutation it was crossed with. One new allele of try-5 and of snf-10 has been found. It is suspected that the other strains contain alleles of new genes not previously identified. Further complementation tests will be done to test this theory.
Toxin-Degrading Bacteria: Herbivorous Rodents May Provide a Source of Novel Microbes for Agricultural Herbivores
Ashley Stengel, University of Utah Biology Agricultural herbivores, such as cattle, often encounter plants containing toxins. One class of toxins, tannins, bind to proteins and inhibit digestive enzymes from acting to liberate nutrients. In this way, tannins prevent optimal absorption of nutrients. To overcome this challenge, some herbivores host bacteria with the ability to degrade tannins. Currently, there is a push to find novel microbes capable of aiding animals in detoxifying these compounds. Therefore, we aimed to isolate tannin-degrading bacteria from the desert woodrat (Neotoma lepida), a wild, herbivorous rodent that feeds largely on a tannin-rich shrub, creosote bush (Larrea tridentata). Woodrat feces were cultured on tannin-treated media, and bacteria capable of degrading tannins were further characterized with DNA sequencing. Results revealed that from 9 isolates, three species of tannin-degrading bacteria were present: Enterococcus faecalis, Bacillus subtilis, and Escherichia coli. Further characterization was performed through measurement of tannase activity. The activity of each isolate varied significantly between bacterial species as well as within a species. Ultimately, I hypothesize that these tannin-degrading bacteria facilitate the ingestion of tannin-rich plants by woodrats. Additionally, this research exemplifies how wild herbivores, such as the desert woodrat, provide an essential source of tannin-degrading microbes that could be introduced into domestic herbivores in order to improve agricultural practices.
Impairment of Withholding a “Pre-Potent” Response In Rats With METH-induced Neurotoxicity
Lee Leavitt, University of Utah Biology Chronic methamphetamine (METH) abuse leads to structural and functional damage in the brain, which likely contributes to cognitive and behavioral dysfunction. Recent data suggest an association between METH abuse and impaired inhibitory control over behavior; that is an impaired ability to inhibit inappropriate actions or thoughts. However, the extent to which METH-induced neurotoxicity is responsible for such impairment remains to be determined. Previously, we reported that rats with METH-induced partial dopamine (DA) and serotonin (5-HT) loss in striatum and prefrontal cortex (PFC) exhibited impaired response inhibition in the form of perseverative responding. Furthermore, levels of serotonin transporter (SERT) binding in PFC correlated with perseverative responding. Herein, we have examined another dimension of response inhibition impulsive action, which is an inability to withhold a “pre-potent” response in rats with METH-induced neurotoxicity. Rats were trained to perform a stop-signal task (SST). Once rats achieved stable responding (>80% correct response) on both “Go” and “Stop” trials, they were treated with saline (0.9%), a neurotoxic regimen of METH (4 x 10 mg/kg, 2-hr intervals, s.c.) under normal ambient temperature conditions (“neurotoxic” METH group) or the METH regimen under cooling conditions (“normothermic” METH group). One week after the treatment, rats were again tested on the SST. After behavioral tests were done, animals were sacrificed and brains removed for determination of monoamine loss. The results showed: 1) the “neurotoxic” METH-, but not the “normothermic” METH or saline-treated rats, showed a 40-60% loss of SERT and dopamine transporter (DAT) binding in PFC and striatum; 2) the “neurotoxic” METH group showed normal behavioral performance in “Go” trials of the SST relative to the “normothermic” METH group or saline controls; 3) the “neurotoxic” METH group exhibited impaired withholding of a “pre-potent” response, as reflected by increased numbers of errors on the “Stop” trials of the SST. Overall, these data suggest that impaired inhibitory control over behavior (i.e., increased impulsive action) can arise as a consequence of METH-induced neurotoxicity to central dopamine and serotonin systems. Supported by NIH grant DA 024036
Photobiology: Optimizing Light Quality to Maximize Plant Growth and Development
Kevin Cope, Utah State University Plants, Soils, and Climate Photosynthesis is driven primarily by radiation between 400 and 700 nm; however, not all wavelengths are equally efficient. Red light (600 to 700 nm) is 25 to 35% more efficient than blue light (400 to 500 nm) and 5 to 30% more efficient than green light (500 to 600 nm). Although blue light is less efficient than red light, it has been shown to be necessary for normal plant development in all tested crop species. The mixture of pigments in plant leaves allows them to absorb all colors of light. Both red and blue light are absorbed primarily in the upper leaf. Green light penetrates deep into the lower leaf and transmits to leaf layers below the upper leaf canopy. Accordingly, once the upper canopy is saturated with red and blue light, supplemental green light is beneficial in increasing whole plant photosynthesis. Although the effect of spectral quality on single leaves is well characterized, the effect on whole plant growth and development is poorly understood. The narrow spectral output of LEDs makes them particularly effective for photobiological studies. From our initial studies with radish, soybean, and wheat, we determined that blue light levels can be used to better predict plant development than red:far-red light ratios. We also found that plants require at least 80 μmol of blue photons m-2 s-1 in order to develop normally. Further studies are currently being conducted to determine the optimal ratio of red and green light for maximizing whole plant photosynthesis in lettuce and radish.
Identifying the Genes that Control Paraquat Resistance in the Roundworm C. elegans
Tyler Shimko, University of Utah Biology Differences in the genomes of organisms control an organism’s ability to deal with and adapt to environmental stresses. In this project, two strain isolates of the nematode Caenorhabditis elegans were analyzed using high-throughput assays measuring growth and offspring production to determine the genes that confer a greater resistance to the herbicide paraquat. Paraquat acts by interfering with electron transport mechanisms within the cells of living organisms. This mechanism not only allows it to act as an effective herbicide, but also causes it to pose a considerable risk to the health of animals, including livestock and humans. Using statistical genetics, regions of the genome were identified that are likely responsible for differences in growth rate and fecundity observed in the two strain isolates when grown in paraquat. Near-isogenic lines and extra-chromosomal arrays were then created to isolate these portions of the genome in a control genetic background. This approach allowed us to be able to attribute any differences in the two traits to the genes contained within the intervals. After analyzing the body size data, representing the growth of the animals over 72 hours, we were able to draw a preliminary conclusion that an interval on chromosome V may have a small but significant effect on growth determination. As a result of this project, a specific interval was identified that may be responsible for a greater growth rate, three near-isogenic lines were created, and 34 extra-chromosomal arrays were generated. This work will be used in the future to identify the gene(s) responsible for the greater growth rate and fecundity observed in some animals exposed to the herbicide paraquat. These results will allow us to draw conclusions about the roles that these genes, and others like them, play in an organism’s ability to cope with environmental stresses.
Experimental Evolution of Feather Louse (Columbicola Columbae) Parasite Body Size
Heidi Montgomery, University of Utah Biology Parasites are arguably the most diverse group of organisms on the planet. One key factor in determining patterns of parasite biodiversity is the number of host species a parasite can infect, or its host specificity. However, surprisingly very little is known about the evolution of host specificity. Here I use domesticated pigeons (Columba livia) and their ectoparasitic feather lice (Columbicola columbae) to demonstrate how parasite body size influences host use. The current study has two main goals: 1) to use different sized hosts to experimentally evolve louse body size, and 2) to determine how parasite body size effects host specificity. Four breeds of domesticated pigeons were infested with parasitic feather lice. After roughly six generations, mean louse body size changed with respect to host body size, with the largest pigeons having the largest lice. Interestingly, the smallest pigeons had larger lice as well. However, this increase in louse size appears to be the result of a change in microhabitat use, with lice shifting from predominantly the wings and tail to the head. This study is one of the first to experimentally demonstrate how changes in parasite morphology and behavior influence the evolution of host specificity.
“Killing Me Softly”: The Economic Toll of Forest Fire Repression
Adam Olson, Brigham Young University Plant and Wildlife Sciences Forests systems of the Central Rocky Mountains rely on an intricate balance of natural disturbance cycles in order to develop properly. Forest fires are one such disturbance, however, certain fire characteristics, particularly fire severity, can vary widely across forest landscapes. In our study, we examined the influence of fire severity on aspen regeneration as well as aspen defense against wildlife and livestock browsing. Our results indicate that high or moderate burn severity is more favorable to aspen regeneration and survival. These areas of severe burn result in a higher density of aspen suckers, more vertical and lateral growth, greater chemical defense concentrations, and less browse damage than the neighboring plots of low or no burn. This data suggests that fire severity should be taken into account when considering plant regeneration and susceptibility to browse damage in burned landscapes.
The Effect of Male Dominance on Female Mate Choice in Poecilia gillii
Michael McEntire, Brigham Young University Biology Female mate choice (intersexual selection) and male dominance interactions (intrasexual selection) can each play important roles in sexual selection. These two mechanisms tend to be discussed in isolation. The goal of this study is to explore the interaction between these two forms of sexual selection. To test this idea, we focused on the livebearing tropical fish system Poecilia gillii. We grouped males into similarly sized pairs and observed them for a week to determine which male was dominant. These pairs were then presented to females in mate choice trials to ascertain female preference. We also photo- graphed the males to determine coloration. We found that females were unable to detect dominant males without viewing the physical contest and that carotenoid coloration bore no effect on female preference. Females tended to choose the male to their left, suggesting the preferential use of their right eyes in making decisions on mate choice.
The Role of Timing of Dietary Selenium and Isoflavone Supplementation in the Reduction of Prostate Cancer Risk Factors in TRAMP Mice
Ji Su Park, Brigham Young University Nutrition, Dietetics, and Food Science Selenium (Se) and soy have each been shown to reduce risk for prostate cancer when consumed at high levels. The purpose of this project was to define the molecular mechanisms of prostate cancer chemoprevention by Se and soy, and to describe how timing of dietary treatment modifies those effects. [C57BL/6 X FVB] F1 TRAMP (TRansgenic Adenocarcinoma of Mouse Prostate) male mice were fed stock diets high or low in soy, with or without a supplement of Se (4.0 mg Se/kg BW as Se-meth- ylselenocysteine) by gavage 5 d/wk in a 2 X 2 factorial design. Mice were exposed to different diets starting from conception, 6 weeks, or 12 weeks of age and were sacrificed at 18 weeks. Three-way ANOVA showed that supplemental Se increased serum and liver Se, with significant interactions with both time and soy intake. Selenium dosing decreased BW independent of soy intake and time of dietary intervention. Both Se and soy decreased epididymal fat pad weights, with Se’s effects being more pronounced in mice exposed to diets from conception than from 6 wk. Urogenital tract weights, a measure of prostate proliferation and tumor volume, were significantly reduced by Se supplementation (P<0.001) and soy (p=0.044), independent of time of dietary intervention. Histological examination of mouse prostates is in progress to determine dietary effects on disease progression. These data suggest that, in this model, chemopreventive efficacy of Se and soy does not differ between prenatal and early post-natal introduction.