Authors: Vy Dao, Devin Taylor, Danielle Taylor
Mentors: Devin Taylor, Danielle Taylor
Insitution: Utah Valley University
Epilepsy is one of the most common neurologic disorders affecting about 60 million people worldwide (Chen et al., Poduri and Lowenstein, 2011). Currently in the field, there are various methods being used for mechanical seizure induction using Drosophila melanogaster. This variability makes it difficult to compare findings from different research studies. We employed various mechanical seizure induction techniques currently used in Drosophila epilepsy research. We focused on both group testing as well as individual testing using different anesthesia parameters. For individual testing, epileptiform activity and locomotor activity were recorded in adult male Drosophila through mechanical seizure induction using a vortex assay at either 24-hours post- or 1-hour post-CO2 anesthesia. We also evaluated the time for 50% of adult drosophila recovery as indicated by a group climbing assay (10 flies per group) following mechanical seizure induction using a vortex assay at either 24-hours post- or 1-hour post-CO2 anesthesia. Finally, for each of the above testing parameters, we not only tested wild-type (WT) flies, but also multiple positive control seizure phenotype flies commonly used in mechanical seizure induction studies using Drosophila melanogaster. We found significant effects in both CO2 induction methods as well as in different genetically induced seizure phenotypes.