Authors: Dante Celani, Laisha Noyes, Candice Johnson, Seun Onileowo, Randy Klabacka
Mentors: Randy Klabacka
Insitution: Utah Tech University
Increased accessibility to next generation sequencing technology (NGS) has facilitated genomic research in diverse biological disciplines studying non-model organisms (which constitute 99.99% of all organisms on Earth). However, most techniques in NGS require mapping of raw-sequencing reads to a refence genome, this presents a challenge for studies involving non-model organisms since no con-specific reference genome is available for the vast majority of these species. Researchers facing this challenge frequently use the reference genome of a related taxon. While it is understood that read mapping efficiency is inversely related to evolutionary distance between read and reference taxa, the exact nature of the relationship is unknown. To examen this we mapped whole genome sequence data from four Aspidoscelis lizard species (“whiptails”) to twenty-eight genomes of varying relatedness across the vertebrate tree of life. We examined the relationship of mapping success to phylogenetic distance and assessed the relationship between these variables.