Authors: Jaycob M Russell, Sujeong Hwang, Jake A Harward, Emily R Morden, Danielle T Taylor, Devin H Taylor
Mentors: Danielle Taylor, Devin Taylor
Insitution: Utah Valley University
Fetal alcohol spectrum disorder (FASD) is estimated to cost four billion dollars per year in the United States that has a wide range of symptoms including low birth weight, microcephaly, and neurobehavioral problems (Belhorma et al., 2021). Drosophila melanogaster is a prime model organism to study FASD as they have variation in sensitivity to alcohol, metabolic and functional tolerance, and withdrawal to ethanol. We collected wild-type (WT) fly embryos and raised them in a regular diet or an antioxidant supplemented diet (acai or curcumin) both with or without ethanol exposure during development. Adult 4-8 day old fruit flies were starved for 24 hours before the conditioning paradigm. The different conditions included exposure to different odors in either an agar environment (CS-) or agar with sucrose (CS+) environment. After conditioning flies were starved again for 24 hours before testing. Y-mazes were used to test their learning and memory abilities. We hypothesized that flies reared on ethanol with the supplemented diet of antioxidants will show an increase in learning and memory performance compared to the FASD condition of flies raised in normal food without antioxidant supplementation, as flies raised in antioxidant supplemented diets will have less oxidative stress. Performance indices for Y-maze tests were calculated based on published protocols: [# No. of flies choosing (CS+) - # No. of flies choosing (CS-) ]/ Total # of flies that made a choice (Mohandasan et al., 2020).