Authors: Melissa Rangel, Avery Larsen, Heath Ogden
Mentors: Heath Ogden
Insitution: Utah Valley University
Mayflies, also known as ephemeroptera, are members of the first group of insects that evolved the ability to fly(Gillott, 2005). Distinct developmental characteristics of the life stages of the mayfly are the reason that Ephemeroptera are of particular interest.The aquatic nymph stages do not have wings, but they do possess abdominal leaf-shaped gills, and in some cases gills associated to the legs. The abdominal gills can sometimes be used to help propel the insect through its environment (Eastham, 1936) in addition to performing gas exchange. Centroptilum triangulifer (family Baetidae), will be used for RNA extractions to study the developmental genetic profiles for characteristics such as wings and gills. Objectives for this research are 1). Acquire samples from different instars, subimago, and imago. 2) Perform 10 RNA extractions. 3) Use Qubit technology to ensure ≥ 20 ng/μL RNA concentration per ≥ 10 μL. 4) samples will be packaged in thermo-stable shipping boxes and sent to Novogene where samples are tested once more for quality control. 5) Once quality is confirmed, samples will be sent and processed by Novogene. 6). Using an in-house bioinformatics workflow, RNA data will be checked for quality, trimmed, aligned, and assembled. The assembled contains will be used to search for genes of interest. 7) the resulting RNA expression profiles will be compared across the installs, sub imago, and imago and to other expression profiles from other related insects.