Authors: Kayla Knaperek, Megan Frisby, Geoffery Zahn, Alma Laney
Mentors: Alma Laney
Insitution: Utah Valley University
In February 2021, mycelial growth was found on blueberries, leading to the obtention of a fungal isolate using potato dextrose agar (PDA) after surface sterilization of the blueberries. The rapid growth of white mycelium and sclerotia was observed on the medium. Inoculation of basil plants with spores from surface-sterilized sclerotia in potato-dextrose broth resulted in necrosis and spreading in terminal buds, ultimately leading to plant death. Further isolation of the identical fungus from the diseased basil tissue confirmed its pathogenicity. Analysis of the isolate's ITS region sequencing revealed a 90% nucleotide identity to the closest relative, Botrytis cinerea. A project was initiated to further characterize the fungus, pathogenicity, life cycle, and morphological traits. The growth and development of the fungus were observed on V8, cornmeal, blueberry, and potato dextrose agars to characterize its nutritional requirements and growth patterns. Host range testing involved the systematic inoculation of various plant species (which were used as growth media), followed by careful symptom monitoring and photographed documentation. Additionally, Koch’s Postulates will be conducted to establish the causal relationship between fungal infection and the observed disease symptoms in the host plants. Seven days after inoculation, necrosis, wilting, and leaf discoloration were observed on select leaves of Lavender var. Oregano, suggesting possible infection by the blueberry fungus. Considering potential host plant immaturity during the initial inoculation, a re-inoculation is planned to ensure successful infection and to obtain conclusive results. Further investigation is warranted to confirm the pathogenic impact of the fungus on host plants.