Authors: Reagan McKee, Vicente Fernández Lara, Jeremy Bakelar, Randy Klabacka, Dustin Kolste
Mentors: Randy Klabacka
Insitution: Utah Tech University
The capability to survive freezing temperatures is of crucial importance to ectothermic organisms inhabiting cold climates. The mechanisms that have evolved to minimalize the cellular damage incurred by freezing are of interest due to their applications in agriculture and medicine. fr10 is a gene identified in the wood frog (Rana sylvatica)that has been shown to reduce the cellular damage sustained in freezing temperatures. To date, fr10 has not been identified in any taxa beyond R. sylvatica and as such, the evolution of this novel gene remains ambiguous. Furthermore, the exact mechanism by which fr10 reduces freezing related damage has yet to be determined. We use bioinformatics scripts to scan 38 frog genomes to identify orthologs in divergent frog lineages across varying elevational and latitudinal gradients and examine the evolution of this novel gene. Understanding the evolutionary and ecological context of this gene can elucidate its function and may inform commercial and medical applications.