Authors: Dallin Zollinger, Lauren Brooks
Mentors: Lauren Brooks
Insitution: Utah Valley University
Antibiotics are one of the most important inventions in recent history, saving countless lives. However, their use has also caused antibiotic resistance to increase in frequency among bacterial populations at an astonishing rate. This increase in frequency will influence our treatment of infections worldwide since previous treatments will no longer be effective, causing many deaths that would once have been preventable. Streptogramins are a class of antibiotics used to treat infections which bacteria have evolved resistance to. This study was focused on the vatA gene, which encodes resistance to streptogramins in Staphylococcus aureus. Samples were collected from various areas on the Utah Valley University campus, the DNA was extracted, and polymerase chain reaction (PCR) specific to the vatA gene was performed. Gel electrophoresis was then performed to visualize the amplicons generated from the PCR to evaluate the presence or absence of the vatA gene. Through this work, we have identified locations where this gene is found.