Authors: Jaylee Montague, Skylar Belka, Gabe Espinoza, Geoff Zahn, Alma Laney
Mentors: Alma Laney
Insitution: Utah Valley University
In May of 2022, a several unidentified trees on Utah Valley University (UVU) campus property exhibited severe virus-like symptoms including: mosaic patterning; yellowing, curling, deformed leaves; and flaking bark. Due to the presence of unidentified aphids and after a literature search, we began investigating the sample for a +ssRNA virus. Extensive testing and RNA sequencing using the MinION v9.4.1 and the cDNA PCR Barcoding kit revealed that neither a ssRNA nor a dsRNA virus were present in the samples, thus, it was suspected that a DNA virus could be the cause. We utilized Rolling Circle Amplification (RCA) on extracted DNA. Following the RCA, gel electrophoresis revealed a strong amplicon larger than 10 kb, suggesting the presence of a DNA virus. Sequencing of the viral genome from the RCA product using the MinION vs10.4.1 chip showed that the mulberry did contain baculoviruses and large DNA viruses. Unfortunately, though, the MinION did not get results for DNA plant viruses, so we sent samples for RNAseq on the Illumina NovaSeq, with results still pending. This is significant since it is likely that the other symptomatic plants near these trees—including redbud and spirea—were infected with the same virus due to RCA amplification in those samples and the presence of the same potential aphid vector. Mechanical inoculations to several herbaceous hosts were done using infected spirea tissue. Furthermore, to our knowledge this is the first report of a DNA virus infecting spirea and redbud in the United States.