2024 Abstracts

Authors: Adriana Bibo, Nicole Losurdo, Nichole Link. Mentors: Nichole Link, Cindy Greaves. Insitution: University of Utah. Microcephaly is a neurodevelopmental disorder characterized by occipital frontal circumference (OFC) more than two standard deviations below the mean. It can be accompanied by comorbidities including intellectual disabilities, seizures, and other developmental phenotypes. Microcephaly is attributed to malnutrition and exposure to toxins or infection during pregnancy, but genetic mutations are also a leading cause. To investigate the genetic mechanisms behind microcephaly, our lab collaborates with a physician who has completed whole exome sequencing for two hundred microcephaly patients. He identified potentially pathogenic human variants, which we are studying using Drosophila melanogaster as our model organism. We screened for loss of function phenotypes through RNAi knockdown of fly orthologs to determine if these genes are necessary for brain development. In our primary screen, we found that RNAi knockdown of Bx42 in neural stem cells or post-mitotic neurons causes significantly reduced brain lobe volume. In our current study, we are assessing potential causes for reduced brain lobe volume. We seek to determine if RNAi knockdown of Bx42 in neural stem cells or neurons affects cell number, proliferation, or death in developing brains.

Authors: Kenneth Smith, Douglas Cook, Carter Noh, Kirsten Steele, Jordan Porter. Mentors: Douglas Cook. Insitution: Brigham Young University. Farmers, breeders, and researchers are working to understand how various mechanical and biological factors affect crop strength and resistance to late-season lodging (breaking of stalks before harvest). Stalk flexural stiffness has been shown to be an accurate predictor of stalk strength. Stiffness measurements are currently collected with a manually-operated device called the DARLING (Device for Assessing Resistance to Lodging IN Grains). This device can take one worker more than an hour to test 120 stalks and is not feasible for use on millions of acres of corn. The purpose of this research project is to develop a method for automating flexural stiffness measurements of maize stalks.We have developed a novel sensor, the “Flipper”, that is attached to a robot and will enable continuous measurement of flexural stiffness of each stalk in a field more quickly than current methods. Our talk will cover the operating principles behind this novel sensor as well as our work on calibration and validation of the sensor. The Flipper uses two sets of strain gauges arranged in a Wheatstone bridge configuration on a thin, aluminum bar. As various corn stalks brush along the bar, the pairs of gauges work together to measure both the force that each stalk exerts on the bar and the position at which the force is exerted. This method has been tested repeatedly on artificial stalks in both a static and dynamic environment. A calibration dataset was taken by clamping the flipper to a workbench and using various masses placed at varying distances to exert several different moments on the Flipper, which were measured by the strain gauges. The known forces and distances were used to calculate calibration constants that could be used to accurately predict the rest of the forces and distances in the dataset. The same calibration constants could then be used to validate the model in a dynamic situation where the Flipper is attached to a robot that is used in farming applications and driven along a row of artificial corn stalks. We hope that the Flipper has a future of furthering the agricultural industry across the country.

Authors: Grant Cole, Vitaliy Walker, Daniel Adams, Nathan Twitchell, Melissa Lee, Jennifer Mortensen, Brittney Stockholm, Tatiana LeRoy. Mentors: Claudia Jorgensen. Insitution: Utah Valley University. When exposed to stressful situations, the human nervous system reacts with the fight or flight response activation. Physiological indicators of the activation of the fight or flight response include a change in body temperature, increased heart rate, blood pressure, and sweating (Sriram et.al, 2012). There is mounting evidence implicating stress as physiologically and psychologically harmful. For example, recent research has found a correlation between chronic exposure to stress and the development of mental illnesses such as anxiety and depression. Furthermore, chronic stress has the potential to change the anatomy of the brain along the hypothalamic pituitary adrenal axis (HPA), which is an important part of the neuroendocrine system, plays a role in the release of stress hormones, and helps regulate moods, emotions, and sexual behaviorisms (Ramirez, et.al., 2013). As college can be quite stressful, developing healthy coping mechanisms to deal with stress might positively influence the students’ academic performance and it might improve and maintain their psychological and physiological well-being (Skowronek, 2014). Various research studies have been conducted to reveal the therapeutic qualities of music. Music can effectively relieve nervousness, promote mental health, and positively affect students' psychological state (Chi, 2020). Current research on stress-related outcomes shows that music interventions play a significant role in stress reduction on physiological levels (de Witte et. al, 2020).  Preliminary results indicated that physiological responses (including heart rate, electrodermal skin response, and body temperature) to a mathematical calculation task were not impacted by the type of background noise (control, calm music, or upbeat music). Therefore, the current study is while being exposed to various music genre types (Kirschbaum et al., 1993). We hypothesize that calmer music might reduce stress response, while faster and more upbeat music might increase the stress response. The study will employ a between-subject measures design. After obtaining the baseline physiological measures when no music is played, the participant will either be exposed to two segments of relaxation/mediation music or two segments of fast-beat music. While exposed to different music conditions, the physiological response will be used to compare the impact of music genre (calm meditation music versus techno music) as well as practice (music segment 1 versus music segment 2) on the human stress response. Findings on the effects of music manipulation on the human nervous system can be used as additional tools for the management of stress.

Authors: Cole Kempton, Jim Sprinkle, Randy Larsen, Jameson Brennan, Dan Lauritzen, Sawyer Fonnesbeck, Justin Hatch, April Hulet. Mentors: April Hulet. Insitution: Brigham Young University. In the late fall, or dormant season for perennial herbaceous vegetation, cattle grazing may be used for vegetation management to reduce non-native annual grasses in sagebrush steppe ecosystems. However, little is known about cattle distribution and cattle dietary selection during the dormant season. We utilized data obtained from collars equipped with Global Positioning System (GPS) units to determine resource selection by domestic cattle on late fall sagebrush steppe habitat east of Malad, Idaho. GPS data were collected from late-October to mid-November in 2021 and 2022 and paired with slope, elevation, aspect, ruggedness, heat index, distance to water, vegetation, daily maximum temperature, and daily precipitation to determine what influences habitat selection of cattle. Our preliminary analyses showed that cattle selected for higher elevations in 2021 compared to 2022 (1713m and 1670m, respectively; p < 0.0001), spent less time on north slopes in 2021 versus 2022 (57% and 68% of time, respectively; p < 0.0001), and spent less time on slopes greater than 15% in 2021 versus 2022 (an average of 11.9hrs per day and 13.3hrs per day, respectively; p < 0.0001). Interestingly, our data suggests that cattle spent more time on north slopes but at lower elevations in 2022, compared to 2021 where they spent more time on south slopes but at higher elevations. 2021 was also warmer than 2022 on average (2021 average air temperature was 5.1°C, whereas 2022 average air temperature was -0.8°C). Additionally, total precipitation was 2-fold greater in 2021 (52 mm) than total precipitation in 2022 (25mm). Understanding how livestock utilize available resources in relation to environmental factors and climatic patterns can inform the development of more efficient grazing plans. This knowledge can also enhance the use of cattle grazing as a valuable tool in range management and improvement strategies.

Authors: Levi Pouwer, Keaton Helquist. Mentors: Jared Nielsen. Insitution: Brigham Young University. OBJECTIVE: To identify brain regions and networks underlying acquired-brain-injury-induced insomnia. BACKGROUND: In patients of tertiary-level mental healthcare facilities, symptoms of chronic insomnia are prevalent in 78.2% of the population. Additionally, among those who suffer from acquired brain injury, few symptoms are as generally pervasive as that of chronic insomnia. Meta-analyses of insomnia patients with fMRI data have failed to identify consistently affected brain regions. Individual studies have suggested several brain regions are involved in insomnia, including the anterior cingulum, orbitofrontal cortex, the insula, caudate nucleus, and the anterior capsula interna. However, few regions have consistently been implicated in the pathophysiology of insomnia. Moreover, little is known regarding the collective brain networks involving insomnia. DESIGN/METHODS: A comprehensive systematic literature review was conducted to identify case studies of individuals afflicted with acquired brain injury-induced insomnia (N=53). The review process was facilitated by employing Cadima, specialized literature review software. To establish a reference baseline for comparison, a substantial cohort of resting-state functional MRI (fMRI) scans from healthy control subjects (N=1000) was meticulously acquired. Subsequently, lesion network mapping analysis, as outlined by Fox et al. in 2018, was applied to the lesions observed in the 53 patients. This method allowed us to assess the impact on functional connectivity and analyze its implications by comparing the patients' lesion networks with the baseline scans obtained from healthy controls. RESULTS: In the context of lesion network mapping analysis, our study revealed that 45 out of 53 patients diagnosed with acquired brain injury-induced insomnia exhibited brain lesions that were functionally connected to both the left amygdala and the left anterior insula. Moreover, in 39 out of 57 patients, the lesion networks also included bilateral anterior insula and the hypothalamus. Comparative analysis of these lesions with a normative database unveiled a substantial disparity in functional connectivity to the left amygdala and left anterior insula in patients suffering from acquired brain injury-induced insomnia. This finding underscores the relevance of tissue within these regions and tissues functionally connected to these areas in relation to the onset of lesion-induced insomnia. CONCLUSION: The observed functional network connectivity among the left amygdala and the left insular region in relation to insomnia introduces a potentially more intricate mechanism underlying the onset of insomnia, in addition to the previously proposed factors such as hyperarousal in cortical regions and subcortical neuropathologies. Our findings highlight the possibility of a deeper connection between the initiation of insomnia and limbic structures, specifically emphasizing their role in emotion and sleep regulation. This suggests that the neural mechanisms influencing anxiety may have interrelated effects on the mechanisms influencing insomnia. It is evident that further investigation, particularly among psychiatric patients afflicted with insomnia, is warranted to delve into the activities occurring in these regions and to elucidate the potential interplay between anxiety and insomnia. This research paves the way for a deeper understanding of the complex relationship between neural pathways and the development of insomnia, ultimately offering valuable insights for future clinical and therapeutic considerations.

Authors: Amanda Carlson. Mentors: Bradford Berges. Insitution: Brigham Young University. Human Immunodeficiency Virus (HIV) causes AIDS and is one of the most studied viruses in history. HIV is a retrovirus that has two copies of a single stranded RNA genome. While there is in-depth understanding of the virus and its pathogenesis, no completely effective treatment or vaccine exists. One potential target for therapeutic treatment of HIV is Viral Protein R (Vpr). Vpr is a multi-functional accessory protein encoded by the HIV genome. While HIV is a quickly mutating virus, the vpr gene remains relatively conserved. Mutations in this protein dramatically impact the rate of AIDS progression compared to the wild type (WT) version of Vpr. The Vpr polymorphism R77Q is associated with the Long Term Non Progressor (LTNP) phenotype. Regular AIDS onset is 5-7 years for WT virus and 10 or more years for R77Q. These differences in AIDS progression have been observed in vivo by following people with HIV over time. We have successfully shown that R77Q activates G2 cell cycle arrest more efficiently than WT followed by apoptosis, a death mechanism with less inflammation compared to necrosis. While the molecular mechanism of Vpr-induced apoptosis is known, it is not yet determined why point mutations in Vpr are changing levels of apoptosis. With further experimentation, we have shown that R77Q has decreased expression of pro-inflammatory cytokines compared to WT virus, which may explain why it is associated with the LTNP phenotype. The functions of Vpr come from binding and modifying cellular proteins and enzymes. The focus of our research is to determine what molecular interactions change between Vpr mutants to better understand the shifts in apoptotic levels. Vpr can be found intracellularly in the nucleus, cytoplasm, and mitochondria and extracellularly in secreted proteins and within virions. We will determine Vpr concentration in these various locations for both WT Vpr and the R77Q mutant, starting by measuring extracellular Vpr. To quantify virion-associated Vpr, we have designed a research plan. We will use WT-Vpr plasmids tagged by GFP to create GFP-tagged plasmids with either WT, R77Q or null mutations using site-directed mutagenesis. We will use Sanger sequencing for confirmation of the proper Vpr mutations tagged by GFP. We will then digest the plasmid DNA, leaving only the Vpr-GFP component and use PCR to amplify the sequences. We will transfect null virus plasmid (NL4-3) and Vpr-GFP plasmids into HEK cells to package the null virus and Vpr-GFP plasmids together to create active HIV particles. Using these virus particles, we will infect Hut-78 cells for a short time to allow the virion to enter the cells. We will then measure GFP fluorescence via flow cytometry, allowing us to quantify virion Vpr. This will be run alongside a mock infection as a control. We hypothesize that differences in virion Vpr concentrations exist among Vpr mutants. Through these experiments, we aim to discover more about the role Vpr plays in cell death by apoptosis and contribute to the existing literature exploring the importance of Vpr in HIV-1.

Authors: Kate Coneys, Nick Top, Russ Bailey, Logan Walker, Julia Toomey. Mentors: Russ Bailey. Insitution: Utah Valley University. Background: Past literature has emphasized the importance of practicing culturally competent care and has shown that higher clinician cultural humility, as well as a lower frequency of microaggressions occurring in therapy, have been associated with more desirable therapeutic outcomes in retrospective, cross-sectional studies. However, research has also shown that patient outcomes appear to be unrelated to their therapist's experience level (Erekson et al., 2017), suggesting that more experience may not lead to being a more effective therapist, including more culturally humble. Despite the breadth of research on the significance of cultural competence, less is known about how a therapist's cultural humility changes across treatment with a particular client, or how a therapist’s cultural humility changes over an extended period. The aim of the current study is to examine this concept by analyzing both the relationship between the number of sessions spent working with a given therapist and a client’s rating of the clinician’s cultural humility, as well as the relationship between the average cultural humility rating of the therapist and time. We will also examine how the impact of microaggressions occurring in therapy changes during the course of therapy. Hypotheses: It is hypothesized that the results of this study will show a positive association between the number of sessions with the same therapist and their cultural humility, as rated by the client, as well as a negative association between the number of sessions and the impact of microaggressions reported by the client. Additionally, it is hypothesized that the therapist’s cultural humility rating over the entire course of data collection will not change to a statistically significant degree. Methods: We used archival data collected by Utah Valley University’s Student Health Services Counseling Center from August 2021 – September 2023. This archival dataset consists of 564 clients with at least one cultural humility and microaggression data point. Beginning at the third session, and occurring again every four sessions, clients were asked to rate their clinician’s cultural humility, using The Cultural Humility Scale (Hook et al., 2013), and report the frequency and impact of microaggressions that had occurred during the last four sessions. We will analyze the data using multi-level latent growth modelling. Results: Results will be presented as part of the poster presentation. Discussion: If the results of this study agree with the hypothesis, the implications may be that cultural humility improves over time with specific clients, potentially explained by growth of therapeutic alliance over time. However, it may also imply that a therapist’s cultural humility does not improve over extended time periods, showing that cultural humility appears to be static rather than dynamic. If the results do not agree with the hypothesis, this may imply that a client’s perception of their therapist’s cultural humility is static, but that over extended periods of time a therapist’s cultural humility can improve, implying that it is trainable. Sources: Erekson, D.M., Janis, R., Bailey, R.J., Cattani, K., Pedersen, T.R. (2017). A longitudinal investigation of the impact of psychotherapist training: Does training improve client outcomes? Journal of Counseling Psychology, 64(5), 514-524. https://doi.org/10.1037/cou0000252 Hook, J.N., Davis, D.E., Owen, J., Worthington, E.L., Utsey, S.O. (2013). Cultural humility: measuring openness to culturally diverse clients. Journal of Counseling Psychology, 60(3), 353-366. https://doi.org/10.1037/a0032595